AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1375620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375620 MtrunA17_Chr3g0145951 99.119 227 2 0 1 227 1 227 1.98e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1375620 AT1G16210.1 73.009 226 58 1 1 223 1 226 1.16e-106 307

Find 97 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAGTGTATTTAAAATT+TGG 0.164169 tig0019730:-51715 None:intergenic
CGCCTCTTCCTCCCTCTTCT+TGG 0.214378 tig0019730:-50736 None:intergenic
AAGACAAGTTCAAAGATTAT+TGG 0.240090 tig0019730:-51664 None:intergenic
CGTTTCCGCGGCGCTCTTGC+GGG 0.269884 tig0019730:-50637 None:intergenic
CGCCGCGGAAACGGAGAAAA+AGG 0.310222 tig0019730:+50647 Msa1375620:CDS
AGGACATGATTTGGAAGCTT+TGG 0.331210 tig0019730:+51294 Msa1375620:CDS
GCATCTTTAATTCACCTAGT+AGG 0.334052 tig0019730:-50572 None:intergenic
ACCCTGTTTGCCTTCTTGTC+AGG 0.352573 tig0019730:-50849 None:intergenic
GGTTTCTCTTGCTTCAACTT+AGG 0.359376 tig0019730:-51248 None:intergenic
CACAGTACAAGGACATGATT+TGG 0.361884 tig0019730:+51285 Msa1375620:CDS
CGCCTCTCTGGATGCCTGTC+GGG 0.367827 tig0019730:-51098 None:intergenic
CAAAGATTATTGGTTGTAGT+AGG 0.367989 tig0019730:-51654 None:intergenic
CGCCAAGAAGAGGGAGGAAG+AGG 0.386559 tig0019730:+50734 Msa1375620:CDS
CCGCAAGAGCGCCGCGGAAA+CGG 0.388342 tig0019730:+50638 Msa1375620:CDS
GAAGCCTTGAGGCGCCTCTC+TGG 0.388885 tig0019730:-51110 None:intergenic
GAGTGAGTGACTTGATTAAG+AGG 0.390157 tig0019730:-51335 None:intergenic
CCTAGTAGGGTTTCGCTCGC+TGG 0.400470 tig0019730:-50558 None:intergenic
GCCTCCGTCACCTTCGGTAC+AGG 0.404250 tig0019730:-50879 None:intergenic
GGTGCGTCGATTGGCGGAGC+TGG 0.407431 tig0019730:+50794 Msa1375620:CDS
CCGTTTCCGCGGCGCTCTTG+CGG 0.414275 tig0019730:-50638 None:intergenic
CATCTTTAATTCACCTAGTA+GGG 0.414363 tig0019730:-50571 None:intergenic
AGCCTGACAAGAAGGCAAAC+AGG 0.415814 tig0019730:+50847 Msa1375620:CDS
AAAGATGGGTGTGAATTCTA+AGG 0.416000 tig0019730:+50599 Msa1375620:CDS
GGAGGAAGAGGCGGATAAGA+AGG 0.418038 tig0019730:+50746 Msa1375620:CDS
CTTGCGGGCCCTCGCCGCCT+CGG 0.418040 tig0019730:-50622 None:intergenic
GGATAAGAAGGCGGAGGCGG+CGG 0.424210 tig0019730:+50758 Msa1375620:CDS
GAGAGAAGGAGGAGCAGTAC+TGG 0.439791 tig0019730:+50682 Msa1375620:CDS
ACTTGATTAAGAGGATTGTC+AGG 0.442090 tig0019730:-51326 None:intergenic
GCAAGCTATGGCACAGAAGA+AGG 0.443447 tig0019730:+50929 Msa1375620:CDS
GGCGGATAAGAAGGCGGAGG+CGG 0.454208 tig0019730:+50755 Msa1375620:CDS
GAAGAGGACTGCTGGGGAGG+AGG 0.460637 tig0019730:+50965 Msa1375620:CDS
TTCTAAGGCCGAGGCGGCGA+GGG 0.465736 tig0019730:+50614 Msa1375620:CDS
GAAGCGAAGAAGAGGACTGC+TGG 0.465817 tig0019730:+50957 Msa1375620:CDS
GCGCCTCAAGGCTTCCTTCA+AGG 0.472754 tig0019730:+51118 Msa1375620:CDS
AGCGGAGGTGCGTCGATTGG+CGG 0.475781 tig0019730:+50788 Msa1375620:CDS
AGCTCCGCCTCCGTCACCTT+CGG 0.476514 tig0019730:-50885 None:intergenic
AAAACAGATACATACCTTGA+AGG 0.481347 tig0019730:-51132 None:intergenic
GCGCCTCTCTGGATGCCTGT+CGG 0.482921 tig0019730:-51099 None:intergenic
TACTGTGTGTATGTAAGCCC+TGG 0.485764 tig0019730:-51269 None:intergenic
GGAGGCGGCGGCACGACGAG+CGG 0.491424 tig0019730:+50770 Msa1375620:CDS
AAGCGAAGAAGAGGACTGCT+GGG 0.493135 tig0019730:+50958 Msa1375620:CDS
GATGGCTTCGTCAAGAGTGC+TGG 0.493876 tig0019730:-51045 None:intergenic
CGGAGGCGGAGCTGAGGAAG+CGG 0.494922 tig0019730:+50895 Msa1375620:CDS
CCAGCGAGCGAAACCCTACT+AGG 0.505208 tig0019730:+50558 Msa1375620:five_prime_UTR
GCTGAGGAAGCGGCGAGAGG+AGG 0.505549 tig0019730:+50905 Msa1375620:CDS
TCCCTCTTCTTGGCGGCGCG+TGG 0.507668 tig0019730:-50726 None:intergenic
CTCTTCCTCCCTCTTCTTGG+CGG 0.507806 tig0019730:-50733 None:intergenic
AGATTATTGGTTGTAGTAGG+AGG 0.510390 tig0019730:-51651 None:intergenic
GCCACGCGCCGCCAAGAAGA+GGG 0.513629 tig0019730:+50725 Msa1375620:CDS
AGCCACGCGCCGCCAAGAAG+AGG 0.516989 tig0019730:+50724 Msa1375620:CDS
GCGAGAGGAGGAGCAAGCTA+TGG 0.521774 tig0019730:+50917 Msa1375620:CDS
ACGAGCGGAGGTGCGTCGAT+TGG 0.522062 tig0019730:+50785 Msa1375620:CDS
GGCGGCGGCACGACGAGCGG+AGG 0.533711 tig0019730:+50773 Msa1375620:CDS
ACTTAATCATGAATTTGAGA+TGG 0.534693 tig0019730:-51502 None:intergenic
CCTCCGTCACCTTCGGTACA+GGG 0.541244 tig0019730:-50878 None:intergenic
GGAGCTGAGGAAGCGGCGAG+AGG 0.552884 tig0019730:+50902 Msa1375620:CDS
GCCTGACAAGAAGGCAAACA+GGG 0.555319 tig0019730:+50848 Msa1375620:CDS
GCTTACATACACACAGTACA+AGG 0.555636 tig0019730:+51274 Msa1375620:CDS
CAAGAAGAGGGAGGAAGAGG+CGG 0.555830 tig0019730:+50737 Msa1375620:CDS
GGGTGTGAATTCTAAGGCCG+AGG 0.555835 tig0019730:+50605 Msa1375620:CDS
GAAGAAGAGGACTGCTGGGG+AGG 0.559520 tig0019730:+50962 Msa1375620:CDS
AGGTGACGGAGGCGGAGCTG+AGG 0.562961 tig0019730:+50889 Msa1375620:CDS
AGAGGCGGATAAGAAGGCGG+AGG 0.563473 tig0019730:+50752 Msa1375620:CDS
TGTACCGAAGGTGACGGAGG+CGG 0.565468 tig0019730:+50881 Msa1375620:CDS
AGGCGGAGGAAGCGAAGAAG+AGG 0.566562 tig0019730:+50949 Msa1375620:CDS
GATTCAATAAAGTCATGGCT+TGG 0.566810 tig0019730:+51457 Msa1375620:three_prime_UTR
CTCTCTGGATGCCTGTCGGG+AGG 0.568049 tig0019730:-51095 None:intergenic
GCGTCGATTGGCGGAGCTGG+AGG 0.569855 tig0019730:+50797 Msa1375620:CDS
GCATCCAGAGAGGCGCCTCA+AGG 0.574178 tig0019730:+51106 Msa1375620:CDS
ATCGATACTCATCTGAGCGA+TGG 0.576376 tig0019730:-51063 None:intergenic
AGCAGCAACTGCTGAAGCAG+AGG 0.579802 tig0019730:+50518 Msa1375620:five_prime_UTR
ATTCTAAGGCCGAGGCGGCG+AGG 0.588501 tig0019730:+50613 Msa1375620:CDS
TCGGGAGACTCGAGAGAAGG+AGG 0.591271 tig0019730:+50671 Msa1375620:CDS
TCAATAAAGTCATGGCTTGG+CGG 0.593024 tig0019730:+51460 Msa1375620:three_prime_UTR
ATAACAACTTACCTCCCGAC+AGG 0.598312 tig0019730:+51084 Msa1375620:CDS
ACGCGCCGCCAAGAAGAGGG+AGG 0.598349 tig0019730:+50728 Msa1375620:CDS
GAGGGCCCGCAAGAGCGCCG+CGG 0.599591 tig0019730:+50632 Msa1375620:CDS
GGAGCTGGAGGAGAAAGAGC+TGG 0.599937 tig0019730:+50809 Msa1375620:CDS
AAGTTGAAGCAAGAGAAACC+AGG 0.604189 tig0019730:+51251 Msa1375620:CDS
GGATCGGGAGACTCGAGAGA+AGG 0.606508 tig0019730:+50668 Msa1375620:CDS
CCCTGTACCGAAGGTGACGG+AGG 0.608676 tig0019730:+50878 Msa1375620:CDS
TATGGCACAGAAGAAGGCGG+AGG 0.610578 tig0019730:+50935 Msa1375620:CDS
ATGATGATTCAATAAAGTCA+TGG 0.612785 tig0019730:+51452 Msa1375620:three_prime_UTR
GGAGGAGGAGTACGAGAGAG+TGG 0.614209 tig0019730:+50980 Msa1375620:CDS
GGTGTCTATCCCTGTACCGA+AGG 0.615935 tig0019730:+50869 Msa1375620:CDS
TTATTGAATCATCATTCACT+CGG 0.617967 tig0019730:-51444 None:intergenic
GGAAGAGGCGGATAAGAAGG+CGG 0.620199 tig0019730:+50749 Msa1375620:CDS
TATCCCTGTACCGAAGGTGA+CGG 0.630098 tig0019730:+50875 Msa1375620:CDS
CTCCCGACAGGCATCCAGAG+AGG 0.637354 tig0019730:+51096 Msa1375620:CDS
GGAGGAGCAGTACTGGCGAG+AGG 0.649799 tig0019730:+50689 Msa1375620:CDS
TCATATACTACTGCTTACAG+AGG 0.659085 tig0019730:-51559 None:intergenic
TGTGAATTCTAAGGCCGAGG+CGG 0.660597 tig0019730:+50608 Msa1375620:CDS
AGTTGAAGCAAGAGAAACCA+GGG 0.661223 tig0019730:+51252 Msa1375620:CDS
CATATACTACTGCTTACAGA+GGG 0.665172 tig0019730:-51558 None:intergenic
AGCGAAGAAGAGGACTGCTG+GGG 0.681467 tig0019730:+50959 Msa1375620:CDS
ATACCTTGAAGGAAGCCTTG+AGG 0.684866 tig0019730:-51121 None:intergenic
AGCTATGGCACAGAAGAAGG+CGG 0.695332 tig0019730:+50932 Msa1375620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAACAAAAATAAAAAGAT+AGG - tig0019730:51389-51408 None:intergenic 15.0%
!! GATAACAAAAATAAAAAGAT+AGG - tig0019730:51389-51408 None:intergenic 15.0%
!!! TATCGAGTGTATTTAAAATT+TGG - tig0019730:51718-51737 None:intergenic 20.0%
!!! TATCGAGTGTATTTAAAATT+TGG - tig0019730:51718-51737 None:intergenic 20.0%
! AATTCATTCATTCATTCATC+AGG + tig0019730:51205-51224 Msa1375620:intron 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG - tig0019730:51379-51398 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG - tig0019730:51447-51466 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG + tig0019730:51452-51471 Msa1375620:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - tig0019730:51505-51524 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + tig0019730:51611-51630 Msa1375620:three_prime_UTR 25.0%
! AAGACAAGTTCAAAGATTAT+TGG - tig0019730:51667-51686 None:intergenic 25.0%
! AATTCATTCATTCATTCATC+AGG + tig0019730:51205-51224 Msa1375620:intron 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG - tig0019730:51379-51398 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG - tig0019730:51447-51466 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG + tig0019730:51452-51471 Msa1375620:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - tig0019730:51505-51524 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + tig0019730:51611-51630 Msa1375620:three_prime_UTR 25.0%
! AAGACAAGTTCAAAGATTAT+TGG - tig0019730:51667-51686 None:intergenic 25.0%
CATCTTTAATTCACCTAGTA+GGG - tig0019730:50574-50593 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + tig0019730:50584-50603 Msa1375620:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + tig0019730:50585-50604 Msa1375620:exon 30.0%
AAAACAGATACATACCTTGA+AGG - tig0019730:51135-51154 None:intergenic 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - tig0019730:51366-51385 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - tig0019730:51369-51388 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - tig0019730:51657-51676 None:intergenic 30.0%
CATCTTTAATTCACCTAGTA+GGG - tig0019730:50574-50593 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + tig0019730:50584-50603 Msa1375620:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + tig0019730:50585-50604 Msa1375620:exon 30.0%
AAAACAGATACATACCTTGA+AGG - tig0019730:51135-51154 None:intergenic 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - tig0019730:51366-51385 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - tig0019730:51369-51388 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - tig0019730:51657-51676 None:intergenic 30.0%
GCATCTTTAATTCACCTAGT+AGG - tig0019730:50575-50594 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - tig0019730:50597-50616 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - tig0019730:50598-50617 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + tig0019730:50599-50618 Msa1375620:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + tig0019730:50839-50858 Msa1375620:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - tig0019730:51329-51348 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG + tig0019730:51457-51476 Msa1375620:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - tig0019730:51561-51580 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - tig0019730:51562-51581 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - tig0019730:51654-51673 None:intergenic 35.0%
GCATCTTTAATTCACCTAGT+AGG - tig0019730:50575-50594 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - tig0019730:50597-50616 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - tig0019730:50598-50617 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + tig0019730:50599-50618 Msa1375620:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + tig0019730:50839-50858 Msa1375620:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - tig0019730:51329-51348 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG + tig0019730:51457-51476 Msa1375620:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - tig0019730:51561-51580 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - tig0019730:51562-51581 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - tig0019730:51654-51673 None:intergenic 35.0%
GGTTTCTCTTGCTTCAACTT+AGG - tig0019730:51251-51270 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + tig0019730:51251-51270 Msa1375620:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + tig0019730:51252-51271 Msa1375620:CDS 40.0%
GCTTACATACACACAGTACA+AGG + tig0019730:51274-51293 Msa1375620:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + tig0019730:51285-51304 Msa1375620:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + tig0019730:51294-51313 Msa1375620:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - tig0019730:51338-51357 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + tig0019730:51460-51479 Msa1375620:three_prime_UTR 40.0%
GGTTTCTCTTGCTTCAACTT+AGG - tig0019730:51251-51270 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + tig0019730:51251-51270 Msa1375620:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + tig0019730:51252-51271 Msa1375620:CDS 40.0%
GCTTACATACACACAGTACA+AGG + tig0019730:51274-51293 Msa1375620:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + tig0019730:51285-51304 Msa1375620:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + tig0019730:51294-51313 Msa1375620:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - tig0019730:51338-51357 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + tig0019730:51460-51479 Msa1375620:three_prime_UTR 40.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - tig0019730:50652-50671 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + tig0019730:50652-50671 Msa1375620:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + tig0019730:50653-50672 Msa1375620:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - tig0019730:51066-51085 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + tig0019730:51084-51103 Msa1375620:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - tig0019730:51124-51143 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - tig0019730:51272-51291 None:intergenic 45.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - tig0019730:50652-50671 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + tig0019730:50652-50671 Msa1375620:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + tig0019730:50653-50672 Msa1375620:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - tig0019730:51066-51085 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + tig0019730:51084-51103 Msa1375620:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - tig0019730:51124-51143 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - tig0019730:51272-51291 None:intergenic 45.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + tig0019730:50608-50627 Msa1375620:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + tig0019730:50847-50866 Msa1375620:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + tig0019730:50848-50867 Msa1375620:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - tig0019730:50852-50871 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + tig0019730:50875-50894 Msa1375620:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + tig0019730:50929-50948 Msa1375620:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + tig0019730:50932-50951 Msa1375620:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + tig0019730:50958-50977 Msa1375620:CDS 50.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + tig0019730:50608-50627 Msa1375620:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + tig0019730:50847-50866 Msa1375620:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + tig0019730:50848-50867 Msa1375620:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - tig0019730:50852-50871 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + tig0019730:50875-50894 Msa1375620:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + tig0019730:50929-50948 Msa1375620:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + tig0019730:50932-50951 Msa1375620:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + tig0019730:50958-50977 Msa1375620:CDS 50.0%
AGCAGCAACTGCTGAAGCAG+AGG + tig0019730:50518-50537 Msa1375620:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + tig0019730:50605-50624 Msa1375620:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + tig0019730:50682-50701 Msa1375620:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - tig0019730:50736-50755 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + tig0019730:50737-50756 Msa1375620:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + tig0019730:50746-50765 Msa1375620:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + tig0019730:50749-50768 Msa1375620:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + tig0019730:50869-50888 Msa1375620:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + tig0019730:50935-50954 Msa1375620:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + tig0019730:50957-50976 Msa1375620:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + tig0019730:50959-50978 Msa1375620:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - tig0019730:51048-51067 None:intergenic 55.0%
AGCAGCAACTGCTGAAGCAG+AGG + tig0019730:50518-50537 Msa1375620:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + tig0019730:50605-50624 Msa1375620:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + tig0019730:50682-50701 Msa1375620:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - tig0019730:50736-50755 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + tig0019730:50737-50756 Msa1375620:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + tig0019730:50746-50765 Msa1375620:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + tig0019730:50749-50768 Msa1375620:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + tig0019730:50869-50888 Msa1375620:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + tig0019730:50935-50954 Msa1375620:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + tig0019730:50957-50976 Msa1375620:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + tig0019730:50959-50978 Msa1375620:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - tig0019730:51048-51067 None:intergenic 55.0%
CCTAGTAGGGTTTCGCTCGC+TGG - tig0019730:50561-50580 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + tig0019730:50558-50577 Msa1375620:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + tig0019730:50647-50666 Msa1375620:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + tig0019730:50668-50687 Msa1375620:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + tig0019730:50671-50690 Msa1375620:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + tig0019730:50734-50753 Msa1375620:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - tig0019730:50739-50758 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + tig0019730:50752-50771 Msa1375620:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + tig0019730:50809-50828 Msa1375620:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - tig0019730:50881-50900 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + tig0019730:50881-50900 Msa1375620:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + tig0019730:50917-50936 Msa1375620:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + tig0019730:50949-50968 Msa1375620:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + tig0019730:50962-50981 Msa1375620:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + tig0019730:50980-50999 Msa1375620:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + tig0019730:51118-51137 Msa1375620:CDS 60.0%
CCTAGTAGGGTTTCGCTCGC+TGG - tig0019730:50561-50580 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + tig0019730:50558-50577 Msa1375620:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + tig0019730:50647-50666 Msa1375620:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + tig0019730:50668-50687 Msa1375620:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + tig0019730:50671-50690 Msa1375620:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + tig0019730:50734-50753 Msa1375620:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - tig0019730:50739-50758 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + tig0019730:50752-50771 Msa1375620:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + tig0019730:50809-50828 Msa1375620:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - tig0019730:50881-50900 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + tig0019730:50881-50900 Msa1375620:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + tig0019730:50917-50936 Msa1375620:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + tig0019730:50949-50968 Msa1375620:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + tig0019730:50962-50981 Msa1375620:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + tig0019730:50980-50999 Msa1375620:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + tig0019730:51118-51137 Msa1375620:CDS 60.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + tig0019730:50613-50632 Msa1375620:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + tig0019730:50614-50633 Msa1375620:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + tig0019730:50689-50708 Msa1375620:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + tig0019730:50755-50774 Msa1375620:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + tig0019730:50758-50777 Msa1375620:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + tig0019730:50785-50804 Msa1375620:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + tig0019730:50788-50807 Msa1375620:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + tig0019730:50878-50897 Msa1375620:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - tig0019730:50882-50901 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - tig0019730:50888-50907 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + tig0019730:50965-50984 Msa1375620:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - tig0019730:51098-51117 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + tig0019730:51096-51115 Msa1375620:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - tig0019730:51101-51120 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - tig0019730:51102-51121 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + tig0019730:51106-51125 Msa1375620:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - tig0019730:51113-51132 None:intergenic 65.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + tig0019730:50613-50632 Msa1375620:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + tig0019730:50614-50633 Msa1375620:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + tig0019730:50689-50708 Msa1375620:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + tig0019730:50755-50774 Msa1375620:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + tig0019730:50758-50777 Msa1375620:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + tig0019730:50785-50804 Msa1375620:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + tig0019730:50788-50807 Msa1375620:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + tig0019730:50878-50897 Msa1375620:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - tig0019730:50882-50901 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - tig0019730:50888-50907 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + tig0019730:50965-50984 Msa1375620:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - tig0019730:51098-51117 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + tig0019730:51096-51115 Msa1375620:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - tig0019730:51101-51120 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - tig0019730:51102-51121 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + tig0019730:51106-51125 Msa1375620:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - tig0019730:51113-51132 None:intergenic 65.0%
CGTTTCCGCGGCGCTCTTGC+GGG - tig0019730:50640-50659 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - tig0019730:50641-50660 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + tig0019730:50638-50657 Msa1375620:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + tig0019730:50724-50743 Msa1375620:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + tig0019730:50725-50744 Msa1375620:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - tig0019730:50729-50748 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + tig0019730:50728-50747 Msa1375620:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + tig0019730:50794-50813 Msa1375620:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + tig0019730:50797-50816 Msa1375620:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + tig0019730:50889-50908 Msa1375620:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + tig0019730:50895-50914 Msa1375620:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + tig0019730:50902-50921 Msa1375620:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + tig0019730:50905-50924 Msa1375620:CDS 70.0%
CGTTTCCGCGGCGCTCTTGC+GGG - tig0019730:50640-50659 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - tig0019730:50641-50660 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + tig0019730:50638-50657 Msa1375620:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + tig0019730:50724-50743 Msa1375620:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + tig0019730:50725-50744 Msa1375620:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - tig0019730:50729-50748 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + tig0019730:50728-50747 Msa1375620:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + tig0019730:50794-50813 Msa1375620:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + tig0019730:50797-50816 Msa1375620:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + tig0019730:50889-50908 Msa1375620:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + tig0019730:50895-50914 Msa1375620:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + tig0019730:50902-50921 Msa1375620:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + tig0019730:50905-50924 Msa1375620:CDS 70.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - tig0019730:50625-50644 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + tig0019730:50632-50651 Msa1375620:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + tig0019730:50770-50789 Msa1375620:CDS 80.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - tig0019730:50625-50644 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + tig0019730:50632-50651 Msa1375620:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + tig0019730:50770-50789 Msa1375620:CDS 80.0%
! GGCGGCGGCACGACGAGCGG+AGG + tig0019730:50773-50792 Msa1375620:CDS 85.0%
! GGCGGCGGCACGACGAGCGG+AGG + tig0019730:50773-50792 Msa1375620:CDS 85.0%
Chromosome Type Strat End Strand Name
tig0019730 gene 50499 51762 50499 ID=Msa1375620;Name=Msa1375620
tig0019730 mRNA 50499 51762 50499 ID=Msa1375620-mRNA-1;Parent=Msa1375620;Name=Msa1375620-mRNA-1;_AED=0.05;_eAED=0.05;_QI=92|1|1|1|1|1|3|311|227
tig0019730 exon 50499 51139 50499 ID=Msa1375620-mRNA-1:exon:4596;Parent=Msa1375620-mRNA-1
tig0019730 exon 51227 51346 51227 ID=Msa1375620-mRNA-1:exon:4597;Parent=Msa1375620-mRNA-1
tig0019730 exon 51437 51762 51437 ID=Msa1375620-mRNA-1:exon:4598;Parent=Msa1375620-mRNA-1
tig0019730 five_prime_UTR 50499 50590 50499 ID=Msa1375620-mRNA-1:five_prime_utr;Parent=Msa1375620-mRNA-1
tig0019730 CDS 50591 51139 50591 ID=Msa1375620-mRNA-1:cds;Parent=Msa1375620-mRNA-1
tig0019730 CDS 51227 51346 51227 ID=Msa1375620-mRNA-1:cds;Parent=Msa1375620-mRNA-1
tig0019730 CDS 51437 51451 51437 ID=Msa1375620-mRNA-1:cds;Parent=Msa1375620-mRNA-1
tig0019730 three_prime_UTR 51452 51762 51452 ID=Msa1375620-mRNA-1:three_prime_utr;Parent=Msa1375620-mRNA-1
Gene Sequence

>Msa1375620

ATGCCCAAAAAGATGGGTGTGAATTCTAAGGCCGAGGCGGCGAGGGCCCGCAAGAGCGCCGCGGAAACGGAGAAAAAGGATCGGGAGACTCGAGAGAAGGAGGAGCAGTACTGGCGAGAGGCAGAGAGTTCAAAGCCACGCGCCGCCAAGAAGAGGGAGGAAGAGGCGGATAAGAAGGCGGAGGCGGCGGCACGACGAGCGGAGGTGCGTCGATTGGCGGAGCTGGAGGAGAAAGAGCTGGAGAAGATGATTAAAAAGCCTGACAAGAAGGCAAACAGGGTGTCTATCCCTGTACCGAAGGTGACGGAGGCGGAGCTGAGGAAGCGGCGAGAGGAGGAGCAAGCTATGGCACAGAAGAAGGCGGAGGAAGCGAAGAAGAGGACTGCTGGGGAGGAGGAGTACGAGAGAGTGGTGCTTGTGTCGAATACGAATCGTGATGATTCAATTATCGAAGCCAGCACTCTTGACGAAGCCATCGCTCAGATGAGTATCGATAACAACTTACCTCCCGACAGGCATCCAGAGAGGCGCCTCAAGGCTTCCTTCAAGGCATTTGAAGAAGCTGAACTACCTAAGTTGAAGCAAGAGAAACCAGGGCTTACATACACACAGTACAAGGACATGATTTGGAAGCTTTGGAAGAAATCTCCTGACAATCCTCTTAATCAAATTGCTGCCGAGTGA
Protein sequence

>Msa1375620

MPKKMGVNSKAEAARARKSAAETEKKDRETREKEEQYWREAESSKPRAAKKREEEADKKAEAAARRAEVRRLAELEEKELEKMIKKPDKKANRVSIPVPKVTEAELRKRREEEQAMAQKKAEEAKKRTAGEEEYERVVLVSNTNRDDSIIEASTLDEAIAQMSIDNNLPPDRHPERRLKASFKAFEEAELPKLKQEKPGLTYTQYKDMIWKLWKKSPDNPLNQIAAE*