AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376540 MtrunA17_Chr3g0145951 99.119 227 2 0 1 227 1 227 1.98e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376540 AT1G16210.1 73.009 226 58 1 1 223 1 226 1.16e-106 307

Find 97 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAGTGTATTTAAAATT+TGG 0.164169 tig0019825:+60207 None:intergenic
CGCCTCTTCCTCCCTCTTCT+TGG 0.214378 tig0019825:+61179 None:intergenic
AAGACAAGTTCAAAGATTAT+TGG 0.240090 tig0019825:+60258 None:intergenic
CGTTTCCGCGGCGCTCTTGC+GGG 0.269884 tig0019825:+61278 None:intergenic
CGCCGCGGAAACGGAGAAAA+AGG 0.310222 tig0019825:-61268 Msa1376540:CDS
AGGACATGATTTGGAAGCTT+TGG 0.331210 tig0019825:-60627 Msa1376540:CDS
GCATCTTTAATTCACCTAGT+AGG 0.334052 tig0019825:+61343 None:intergenic
ACCCTGTTTGCCTTCTTGTC+AGG 0.352573 tig0019825:+61066 None:intergenic
GGTTTCTCTTGCTTCAACTT+AGG 0.359376 tig0019825:+60673 None:intergenic
CACAGTACAAGGACATGATT+TGG 0.361884 tig0019825:-60636 Msa1376540:CDS
CGCCTCTCTGGATGCCTGTC+GGG 0.367827 tig0019825:+60817 None:intergenic
CAAAGATTATTGGTTGTAGT+AGG 0.367989 tig0019825:+60268 None:intergenic
CGCCAAGAAGAGGGAGGAAG+AGG 0.386559 tig0019825:-61181 Msa1376540:CDS
CCGCAAGAGCGCCGCGGAAA+CGG 0.388342 tig0019825:-61277 Msa1376540:CDS
GAAGCCTTGAGGCGCCTCTC+TGG 0.388885 tig0019825:+60805 None:intergenic
GAGTGAGTGACTTGATTAAG+AGG 0.390157 tig0019825:+60586 None:intergenic
CCTAGTAGGGTTTCGCTCGC+TGG 0.400470 tig0019825:+61357 None:intergenic
GCCTCCGTCACCTTCGGTAC+AGG 0.404250 tig0019825:+61036 None:intergenic
GGTGCGTCGATTGGCGGAGC+TGG 0.407431 tig0019825:-61121 Msa1376540:CDS
CCGTTTCCGCGGCGCTCTTG+CGG 0.414275 tig0019825:+61277 None:intergenic
CATCTTTAATTCACCTAGTA+GGG 0.414363 tig0019825:+61344 None:intergenic
AGCCTGACAAGAAGGCAAAC+AGG 0.415814 tig0019825:-61068 Msa1376540:CDS
AAAGATGGGTGTGAATTCTA+AGG 0.416000 tig0019825:-61316 Msa1376540:CDS
GGAGGAAGAGGCGGATAAGA+AGG 0.418038 tig0019825:-61169 Msa1376540:CDS
CTTGCGGGCCCTCGCCGCCT+CGG 0.418040 tig0019825:+61293 None:intergenic
GGATAAGAAGGCGGAGGCGG+CGG 0.424210 tig0019825:-61157 Msa1376540:CDS
GAGAGAAGGAGGAGCAGTAC+TGG 0.439791 tig0019825:-61233 Msa1376540:CDS
ACTTGATTAAGAGGATTGTC+AGG 0.442090 tig0019825:+60595 None:intergenic
GCAAGCTATGGCACAGAAGA+AGG 0.443447 tig0019825:-60986 Msa1376540:CDS
GGCGGATAAGAAGGCGGAGG+CGG 0.454208 tig0019825:-61160 Msa1376540:CDS
GAAGAGGACTGCTGGGGAGG+AGG 0.460637 tig0019825:-60950 Msa1376540:CDS
TTCTAAGGCCGAGGCGGCGA+GGG 0.465736 tig0019825:-61301 Msa1376540:CDS
GAAGCGAAGAAGAGGACTGC+TGG 0.465817 tig0019825:-60958 Msa1376540:CDS
GCGCCTCAAGGCTTCCTTCA+AGG 0.472754 tig0019825:-60797 Msa1376540:intron
AGCGGAGGTGCGTCGATTGG+CGG 0.475781 tig0019825:-61127 Msa1376540:CDS
AGCTCCGCCTCCGTCACCTT+CGG 0.476514 tig0019825:+61030 None:intergenic
AAAACAGATACATACCTTGA+AGG 0.481347 tig0019825:+60783 None:intergenic
GCGCCTCTCTGGATGCCTGT+CGG 0.482921 tig0019825:+60816 None:intergenic
TACTGTGTGTATGTAAGCCC+TGG 0.485764 tig0019825:+60652 None:intergenic
GGAGGCGGCGGCACGACGAG+CGG 0.491424 tig0019825:-61145 Msa1376540:CDS
AAGCGAAGAAGAGGACTGCT+GGG 0.493135 tig0019825:-60957 Msa1376540:CDS
GATGGCTTCGTCAAGAGTGC+TGG 0.493876 tig0019825:+60870 None:intergenic
CGGAGGCGGAGCTGAGGAAG+CGG 0.494922 tig0019825:-61020 Msa1376540:CDS
CCAGCGAGCGAAACCCTACT+AGG 0.505208 tig0019825:-61357 Msa1376540:five_prime_UTR
GCTGAGGAAGCGGCGAGAGG+AGG 0.505549 tig0019825:-61010 Msa1376540:CDS
TCCCTCTTCTTGGCGGCGCG+TGG 0.507668 tig0019825:+61189 None:intergenic
CTCTTCCTCCCTCTTCTTGG+CGG 0.507806 tig0019825:+61182 None:intergenic
AGATTATTGGTTGTAGTAGG+AGG 0.510390 tig0019825:+60271 None:intergenic
GCCACGCGCCGCCAAGAAGA+GGG 0.513629 tig0019825:-61190 Msa1376540:CDS
AGCCACGCGCCGCCAAGAAG+AGG 0.516989 tig0019825:-61191 Msa1376540:CDS
GCGAGAGGAGGAGCAAGCTA+TGG 0.521774 tig0019825:-60998 Msa1376540:CDS
ACGAGCGGAGGTGCGTCGAT+TGG 0.522062 tig0019825:-61130 Msa1376540:CDS
GGCGGCGGCACGACGAGCGG+AGG 0.533711 tig0019825:-61142 Msa1376540:CDS
ACTTAATCATGAATTTGAGA+TGG 0.534693 tig0019825:+60420 None:intergenic
CCTCCGTCACCTTCGGTACA+GGG 0.541244 tig0019825:+61037 None:intergenic
GGAGCTGAGGAAGCGGCGAG+AGG 0.552884 tig0019825:-61013 Msa1376540:CDS
GCCTGACAAGAAGGCAAACA+GGG 0.555319 tig0019825:-61067 Msa1376540:CDS
GCTTACATACACACAGTACA+AGG 0.555636 tig0019825:-60647 Msa1376540:CDS
CAAGAAGAGGGAGGAAGAGG+CGG 0.555830 tig0019825:-61178 Msa1376540:CDS
GGGTGTGAATTCTAAGGCCG+AGG 0.555835 tig0019825:-61310 Msa1376540:CDS
GAAGAAGAGGACTGCTGGGG+AGG 0.559520 tig0019825:-60953 Msa1376540:CDS
AGGTGACGGAGGCGGAGCTG+AGG 0.562961 tig0019825:-61026 Msa1376540:CDS
AGAGGCGGATAAGAAGGCGG+AGG 0.563473 tig0019825:-61163 Msa1376540:CDS
TGTACCGAAGGTGACGGAGG+CGG 0.565468 tig0019825:-61034 Msa1376540:CDS
AGGCGGAGGAAGCGAAGAAG+AGG 0.566562 tig0019825:-60966 Msa1376540:CDS
GATTCAATAAAGTCATGGCT+TGG 0.566810 tig0019825:-60465 Msa1376540:three_prime_UTR
CTCTCTGGATGCCTGTCGGG+AGG 0.568049 tig0019825:+60820 None:intergenic
GCGTCGATTGGCGGAGCTGG+AGG 0.569855 tig0019825:-61118 Msa1376540:CDS
GCATCCAGAGAGGCGCCTCA+AGG 0.574178 tig0019825:-60809 Msa1376540:CDS
ATCGATACTCATCTGAGCGA+TGG 0.576376 tig0019825:+60852 None:intergenic
AGCAGCAACTGCTGAAGCAG+AGG 0.579802 tig0019825:-61397 Msa1376540:five_prime_UTR
ATTCTAAGGCCGAGGCGGCG+AGG 0.588501 tig0019825:-61302 Msa1376540:CDS
TCGGGAGACTCGAGAGAAGG+AGG 0.591271 tig0019825:-61244 Msa1376540:CDS
TCAATAAAGTCATGGCTTGG+CGG 0.593024 tig0019825:-60462 Msa1376540:three_prime_UTR
ATAACAACTTACCTCCCGAC+AGG 0.598312 tig0019825:-60831 Msa1376540:CDS
ACGCGCCGCCAAGAAGAGGG+AGG 0.598349 tig0019825:-61187 Msa1376540:CDS
GAGGGCCCGCAAGAGCGCCG+CGG 0.599591 tig0019825:-61283 Msa1376540:CDS
GGAGCTGGAGGAGAAAGAGC+TGG 0.599937 tig0019825:-61106 Msa1376540:CDS
AAGTTGAAGCAAGAGAAACC+AGG 0.604189 tig0019825:-60670 Msa1376540:CDS
GGATCGGGAGACTCGAGAGA+AGG 0.606508 tig0019825:-61247 Msa1376540:CDS
CCCTGTACCGAAGGTGACGG+AGG 0.608676 tig0019825:-61037 Msa1376540:CDS
TATGGCACAGAAGAAGGCGG+AGG 0.610578 tig0019825:-60980 Msa1376540:CDS
ATGATGATTCAATAAAGTCA+TGG 0.612785 tig0019825:-60470 Msa1376540:three_prime_UTR
GGAGGAGGAGTACGAGAGAG+TGG 0.614209 tig0019825:-60935 Msa1376540:CDS
GGTGTCTATCCCTGTACCGA+AGG 0.615935 tig0019825:-61046 Msa1376540:CDS
TTATTGAATCATCATTCACT+CGG 0.617967 tig0019825:+60478 None:intergenic
GGAAGAGGCGGATAAGAAGG+CGG 0.620199 tig0019825:-61166 Msa1376540:CDS
TATCCCTGTACCGAAGGTGA+CGG 0.630098 tig0019825:-61040 Msa1376540:CDS
CTCCCGACAGGCATCCAGAG+AGG 0.637354 tig0019825:-60819 Msa1376540:CDS
GGAGGAGCAGTACTGGCGAG+AGG 0.649799 tig0019825:-61226 Msa1376540:CDS
TCATATACTACTGCTTACAG+AGG 0.659085 tig0019825:+60363 None:intergenic
TGTGAATTCTAAGGCCGAGG+CGG 0.660597 tig0019825:-61307 Msa1376540:CDS
AGTTGAAGCAAGAGAAACCA+GGG 0.661223 tig0019825:-60669 Msa1376540:CDS
CATATACTACTGCTTACAGA+GGG 0.665172 tig0019825:+60364 None:intergenic
AGCGAAGAAGAGGACTGCTG+GGG 0.681467 tig0019825:-60956 Msa1376540:CDS
ATACCTTGAAGGAAGCCTTG+AGG 0.684866 tig0019825:+60794 None:intergenic
AGCTATGGCACAGAAGAAGG+CGG 0.695332 tig0019825:-60983 Msa1376540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAACAAAATAAAAAGAT+AGG + tig0019825:61066-61085 None:intergenic 15.0%
!! AGATAACAAAATAAAAAGAT+AGG + tig0019825:61066-61085 None:intergenic 15.0%
!!! TATCGAGTGTATTTAAAATT+TGG + tig0019825:61394-61413 None:intergenic 20.0%
!!! TATCGAGTGTATTTAAAATT+TGG + tig0019825:61394-61413 None:intergenic 20.0%
! ATAAAAAGATAGGCTAAAAG+TGG + tig0019825:61056-61075 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG + tig0019825:61123-61142 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG - tig0019825:61128-61147 Msa1376540:CDS 25.0%
! ACTTAATCATGAATTTGAGA+TGG + tig0019825:61181-61200 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG - tig0019825:61287-61306 Msa1376540:CDS 25.0%
! AAGACAAGTTCAAAGATTAT+TGG + tig0019825:61343-61362 None:intergenic 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG + tig0019825:61056-61075 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG + tig0019825:61123-61142 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG - tig0019825:61128-61147 Msa1376540:CDS 25.0%
! ACTTAATCATGAATTTGAGA+TGG + tig0019825:61181-61200 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG - tig0019825:61287-61306 Msa1376540:CDS 25.0%
! AAGACAAGTTCAAAGATTAT+TGG + tig0019825:61343-61362 None:intergenic 25.0%
CATCTTTAATTCACCTAGTA+GGG + tig0019825:60257-60276 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG - tig0019825:60267-60286 Msa1376540:three_prime_UTR 30.0%
TTAAAGATGCCCAAAAAGAT+GGG - tig0019825:60268-60287 Msa1376540:three_prime_UTR 30.0%
AAAACAGATACATACCTTGA+AGG + tig0019825:60818-60837 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG - tig0019825:60882-60901 Msa1376540:CDS 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG + tig0019825:61043-61062 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG + tig0019825:61046-61065 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG + tig0019825:61333-61352 None:intergenic 30.0%
CATCTTTAATTCACCTAGTA+GGG + tig0019825:60257-60276 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG - tig0019825:60267-60286 Msa1376540:three_prime_UTR 30.0%
TTAAAGATGCCCAAAAAGAT+GGG - tig0019825:60268-60287 Msa1376540:three_prime_UTR 30.0%
AAAACAGATACATACCTTGA+AGG + tig0019825:60818-60837 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG - tig0019825:60882-60901 Msa1376540:CDS 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG + tig0019825:61043-61062 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG + tig0019825:61046-61065 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG + tig0019825:61333-61352 None:intergenic 30.0%
GCATCTTTAATTCACCTAGT+AGG + tig0019825:60258-60277 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG + tig0019825:60280-60299 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG + tig0019825:60281-60300 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG - tig0019825:60282-60301 Msa1376540:three_prime_UTR 35.0%
GATTAAAAAGCCTGACAAGA+AGG - tig0019825:60522-60541 Msa1376540:intron 35.0%
ACTTGATTAAGAGGATTGTC+AGG + tig0019825:61006-61025 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG - tig0019825:61133-61152 Msa1376540:CDS 35.0%
CATATACTACTGCTTACAGA+GGG + tig0019825:61237-61256 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG + tig0019825:61238-61257 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG + tig0019825:61330-61349 None:intergenic 35.0%
GCATCTTTAATTCACCTAGT+AGG + tig0019825:60258-60277 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG + tig0019825:60280-60299 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG + tig0019825:60281-60300 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG - tig0019825:60282-60301 Msa1376540:three_prime_UTR 35.0%
GATTAAAAAGCCTGACAAGA+AGG - tig0019825:60522-60541 Msa1376540:intron 35.0%
ACTTGATTAAGAGGATTGTC+AGG + tig0019825:61006-61025 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG - tig0019825:61133-61152 Msa1376540:CDS 35.0%
CATATACTACTGCTTACAGA+GGG + tig0019825:61237-61256 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG + tig0019825:61238-61257 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG + tig0019825:61330-61349 None:intergenic 35.0%
GGTTTCTCTTGCTTCAACTT+AGG + tig0019825:60928-60947 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG - tig0019825:60928-60947 Msa1376540:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG - tig0019825:60929-60948 Msa1376540:CDS 40.0%
GCTTACATACACACAGTACA+AGG - tig0019825:60951-60970 Msa1376540:CDS 40.0%
CACAGTACAAGGACATGATT+TGG - tig0019825:60962-60981 Msa1376540:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG - tig0019825:60971-60990 Msa1376540:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG + tig0019825:61015-61034 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG - tig0019825:61136-61155 Msa1376540:CDS 40.0%
GGTTTCTCTTGCTTCAACTT+AGG + tig0019825:60928-60947 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG - tig0019825:60928-60947 Msa1376540:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG - tig0019825:60929-60948 Msa1376540:CDS 40.0%
GCTTACATACACACAGTACA+AGG - tig0019825:60951-60970 Msa1376540:CDS 40.0%
CACAGTACAAGGACATGATT+TGG - tig0019825:60962-60981 Msa1376540:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG - tig0019825:60971-60990 Msa1376540:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG + tig0019825:61015-61034 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG - tig0019825:61136-61155 Msa1376540:CDS 40.0%
! ATCCTTTTTCTCCGTTTCCG+CGG + tig0019825:60335-60354 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG - tig0019825:60335-60354 Msa1376540:three_prime_UTR 45.0%
GGAAACGGAGAAAAAGGATC+GGG - tig0019825:60336-60355 Msa1376540:three_prime_UTR 45.0%
ATCGATACTCATCTGAGCGA+TGG + tig0019825:60749-60768 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG - tig0019825:60767-60786 Msa1376540:intron 45.0%
ATACCTTGAAGGAAGCCTTG+AGG + tig0019825:60807-60826 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG + tig0019825:60949-60968 None:intergenic 45.0%
! ATCCTTTTTCTCCGTTTCCG+CGG + tig0019825:60335-60354 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG - tig0019825:60335-60354 Msa1376540:three_prime_UTR 45.0%
GGAAACGGAGAAAAAGGATC+GGG - tig0019825:60336-60355 Msa1376540:three_prime_UTR 45.0%
ATCGATACTCATCTGAGCGA+TGG + tig0019825:60749-60768 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG - tig0019825:60767-60786 Msa1376540:intron 45.0%
ATACCTTGAAGGAAGCCTTG+AGG + tig0019825:60807-60826 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG + tig0019825:60949-60968 None:intergenic 45.0%
!! TGTGAATTCTAAGGCCGAGG+CGG - tig0019825:60291-60310 Msa1376540:three_prime_UTR 50.0%
AGCCTGACAAGAAGGCAAAC+AGG - tig0019825:60530-60549 Msa1376540:intron 50.0%
GCCTGACAAGAAGGCAAACA+GGG - tig0019825:60531-60550 Msa1376540:intron 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG + tig0019825:60535-60554 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG - tig0019825:60558-60577 Msa1376540:intron 50.0%
GCAAGCTATGGCACAGAAGA+AGG - tig0019825:60612-60631 Msa1376540:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG - tig0019825:60615-60634 Msa1376540:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG - tig0019825:60641-60660 Msa1376540:CDS 50.0%
!! TGTGAATTCTAAGGCCGAGG+CGG - tig0019825:60291-60310 Msa1376540:three_prime_UTR 50.0%
AGCCTGACAAGAAGGCAAAC+AGG - tig0019825:60530-60549 Msa1376540:intron 50.0%
GCCTGACAAGAAGGCAAACA+GGG - tig0019825:60531-60550 Msa1376540:intron 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG + tig0019825:60535-60554 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG - tig0019825:60558-60577 Msa1376540:intron 50.0%
GCAAGCTATGGCACAGAAGA+AGG - tig0019825:60612-60631 Msa1376540:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG - tig0019825:60615-60634 Msa1376540:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG - tig0019825:60641-60660 Msa1376540:CDS 50.0%
AGCAGCAACTGCTGAAGCAG+AGG - tig0019825:60201-60220 Msa1376540:three_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG - tig0019825:60288-60307 Msa1376540:three_prime_UTR 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG - tig0019825:60365-60384 Msa1376540:three_prime_UTR 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG + tig0019825:60419-60438 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG - tig0019825:60420-60439 Msa1376540:three_prime_UTR 55.0%
GGAGGAAGAGGCGGATAAGA+AGG - tig0019825:60429-60448 Msa1376540:three_prime_UTR 55.0%
GGAAGAGGCGGATAAGAAGG+CGG - tig0019825:60432-60451 Msa1376540:three_prime_UTR 55.0%
GGTGTCTATCCCTGTACCGA+AGG - tig0019825:60552-60571 Msa1376540:intron 55.0%
TATGGCACAGAAGAAGGCGG+AGG - tig0019825:60618-60637 Msa1376540:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG - tig0019825:60640-60659 Msa1376540:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG - tig0019825:60642-60661 Msa1376540:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG + tig0019825:60731-60750 None:intergenic 55.0%
AGCAGCAACTGCTGAAGCAG+AGG - tig0019825:60201-60220 Msa1376540:three_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG - tig0019825:60288-60307 Msa1376540:three_prime_UTR 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG - tig0019825:60365-60384 Msa1376540:three_prime_UTR 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG + tig0019825:60419-60438 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG - tig0019825:60420-60439 Msa1376540:three_prime_UTR 55.0%
GGAGGAAGAGGCGGATAAGA+AGG - tig0019825:60429-60448 Msa1376540:three_prime_UTR 55.0%
GGAAGAGGCGGATAAGAAGG+CGG - tig0019825:60432-60451 Msa1376540:three_prime_UTR 55.0%
GGTGTCTATCCCTGTACCGA+AGG - tig0019825:60552-60571 Msa1376540:intron 55.0%
TATGGCACAGAAGAAGGCGG+AGG - tig0019825:60618-60637 Msa1376540:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG - tig0019825:60640-60659 Msa1376540:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG - tig0019825:60642-60661 Msa1376540:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG + tig0019825:60731-60750 None:intergenic 55.0%
CCTAGTAGGGTTTCGCTCGC+TGG + tig0019825:60244-60263 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG - tig0019825:60241-60260 Msa1376540:three_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG - tig0019825:60330-60349 Msa1376540:three_prime_UTR 60.0%
GGATCGGGAGACTCGAGAGA+AGG - tig0019825:60351-60370 Msa1376540:three_prime_UTR 60.0%
TCGGGAGACTCGAGAGAAGG+AGG - tig0019825:60354-60373 Msa1376540:three_prime_UTR 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG - tig0019825:60417-60436 Msa1376540:three_prime_UTR 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG + tig0019825:60422-60441 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG - tig0019825:60435-60454 Msa1376540:three_prime_UTR 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG - tig0019825:60492-60511 Msa1376540:intron 60.0%
CCTCCGTCACCTTCGGTACA+GGG + tig0019825:60564-60583 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG - tig0019825:60564-60583 Msa1376540:intron 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG - tig0019825:60600-60619 Msa1376540:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG - tig0019825:60632-60651 Msa1376540:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG - tig0019825:60645-60664 Msa1376540:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG - tig0019825:60663-60682 Msa1376540:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG - tig0019825:60801-60820 Msa1376540:CDS 60.0%
CCTAGTAGGGTTTCGCTCGC+TGG + tig0019825:60244-60263 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG - tig0019825:60241-60260 Msa1376540:three_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG - tig0019825:60330-60349 Msa1376540:three_prime_UTR 60.0%
GGATCGGGAGACTCGAGAGA+AGG - tig0019825:60351-60370 Msa1376540:three_prime_UTR 60.0%
TCGGGAGACTCGAGAGAAGG+AGG - tig0019825:60354-60373 Msa1376540:three_prime_UTR 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG - tig0019825:60417-60436 Msa1376540:three_prime_UTR 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG + tig0019825:60422-60441 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG - tig0019825:60435-60454 Msa1376540:three_prime_UTR 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG - tig0019825:60492-60511 Msa1376540:intron 60.0%
CCTCCGTCACCTTCGGTACA+GGG + tig0019825:60564-60583 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG - tig0019825:60564-60583 Msa1376540:intron 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG - tig0019825:60600-60619 Msa1376540:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG - tig0019825:60632-60651 Msa1376540:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG - tig0019825:60645-60664 Msa1376540:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG - tig0019825:60663-60682 Msa1376540:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG - tig0019825:60801-60820 Msa1376540:CDS 60.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG - tig0019825:60296-60315 Msa1376540:three_prime_UTR 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG - tig0019825:60297-60316 Msa1376540:three_prime_UTR 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG - tig0019825:60372-60391 Msa1376540:three_prime_UTR 65.0%
GGCGGATAAGAAGGCGGAGG+CGG - tig0019825:60438-60457 Msa1376540:three_prime_UTR 65.0%
GGATAAGAAGGCGGAGGCGG+CGG - tig0019825:60441-60460 Msa1376540:three_prime_UTR 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG - tig0019825:60468-60487 Msa1376540:three_prime_UTR 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG - tig0019825:60471-60490 Msa1376540:three_prime_UTR 65.0%
! CCCTGTACCGAAGGTGACGG+AGG - tig0019825:60561-60580 Msa1376540:intron 65.0%
GCCTCCGTCACCTTCGGTAC+AGG + tig0019825:60565-60584 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG + tig0019825:60571-60590 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG - tig0019825:60648-60667 Msa1376540:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG + tig0019825:60781-60800 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG - tig0019825:60779-60798 Msa1376540:intron 65.0%
CGCCTCTCTGGATGCCTGTC+GGG + tig0019825:60784-60803 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG + tig0019825:60785-60804 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG - tig0019825:60789-60808 Msa1376540:intron 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG + tig0019825:60796-60815 None:intergenic 65.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG - tig0019825:60296-60315 Msa1376540:three_prime_UTR 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG - tig0019825:60297-60316 Msa1376540:three_prime_UTR 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG - tig0019825:60372-60391 Msa1376540:three_prime_UTR 65.0%
GGCGGATAAGAAGGCGGAGG+CGG - tig0019825:60438-60457 Msa1376540:three_prime_UTR 65.0%
GGATAAGAAGGCGGAGGCGG+CGG - tig0019825:60441-60460 Msa1376540:three_prime_UTR 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG - tig0019825:60468-60487 Msa1376540:three_prime_UTR 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG - tig0019825:60471-60490 Msa1376540:three_prime_UTR 65.0%
! CCCTGTACCGAAGGTGACGG+AGG - tig0019825:60561-60580 Msa1376540:intron 65.0%
GCCTCCGTCACCTTCGGTAC+AGG + tig0019825:60565-60584 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG + tig0019825:60571-60590 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG - tig0019825:60648-60667 Msa1376540:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG + tig0019825:60781-60800 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG - tig0019825:60779-60798 Msa1376540:intron 65.0%
CGCCTCTCTGGATGCCTGTC+GGG + tig0019825:60784-60803 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG + tig0019825:60785-60804 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG - tig0019825:60789-60808 Msa1376540:intron 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG + tig0019825:60796-60815 None:intergenic 65.0%
CGTTTCCGCGGCGCTCTTGC+GGG + tig0019825:60323-60342 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG + tig0019825:60324-60343 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG - tig0019825:60321-60340 Msa1376540:three_prime_UTR 70.0%
AGCCACGCGCCGCCAAGAAG+AGG - tig0019825:60407-60426 Msa1376540:three_prime_UTR 70.0%
GCCACGCGCCGCCAAGAAGA+GGG - tig0019825:60408-60427 Msa1376540:three_prime_UTR 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG + tig0019825:60412-60431 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG - tig0019825:60411-60430 Msa1376540:three_prime_UTR 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG - tig0019825:60477-60496 Msa1376540:exon 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG - tig0019825:60480-60499 Msa1376540:exon 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG - tig0019825:60572-60591 Msa1376540:intron 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG - tig0019825:60578-60597 Msa1376540:intron 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG - tig0019825:60585-60604 Msa1376540:intron 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG - tig0019825:60588-60607 Msa1376540:intron 70.0%
CGTTTCCGCGGCGCTCTTGC+GGG + tig0019825:60323-60342 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG + tig0019825:60324-60343 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG - tig0019825:60321-60340 Msa1376540:three_prime_UTR 70.0%
AGCCACGCGCCGCCAAGAAG+AGG - tig0019825:60407-60426 Msa1376540:three_prime_UTR 70.0%
GCCACGCGCCGCCAAGAAGA+GGG - tig0019825:60408-60427 Msa1376540:three_prime_UTR 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG + tig0019825:60412-60431 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG - tig0019825:60411-60430 Msa1376540:three_prime_UTR 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG - tig0019825:60477-60496 Msa1376540:exon 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG - tig0019825:60480-60499 Msa1376540:exon 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG - tig0019825:60572-60591 Msa1376540:intron 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG - tig0019825:60578-60597 Msa1376540:intron 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG - tig0019825:60585-60604 Msa1376540:intron 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG - tig0019825:60588-60607 Msa1376540:intron 70.0%
! CTTGCGGGCCCTCGCCGCCT+CGG + tig0019825:60308-60327 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG - tig0019825:60315-60334 Msa1376540:three_prime_UTR 80.0%
! GGAGGCGGCGGCACGACGAG+CGG - tig0019825:60453-60472 Msa1376540:three_prime_UTR 80.0%
! CTTGCGGGCCCTCGCCGCCT+CGG + tig0019825:60308-60327 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG - tig0019825:60315-60334 Msa1376540:three_prime_UTR 80.0%
! GGAGGCGGCGGCACGACGAG+CGG - tig0019825:60453-60472 Msa1376540:three_prime_UTR 80.0%
! GGCGGCGGCACGACGAGCGG+AGG - tig0019825:60456-60475 Msa1376540:three_prime_UTR 85.0%
! GGCGGCGGCACGACGAGCGG+AGG - tig0019825:60456-60475 Msa1376540:three_prime_UTR 85.0%
Chromosome Type Strat End Strand Name
tig0019825 gene 60182 61438 60182 ID=Msa1376540;Name=Msa1376540
tig0019825 mRNA 60182 61438 60182 ID=Msa1376540-mRNA-1;Parent=Msa1376540;Name=Msa1376540-mRNA-1;_AED=0.05;_eAED=0.05;_QI=92|1|1|1|1|1|3|311|227
tig0019825 exon 60182 60507 60182 ID=Msa1376540-mRNA-1:exon:5226;Parent=Msa1376540-mRNA-1
tig0019825 exon 60597 60716 60597 ID=Msa1376540-mRNA-1:exon:5225;Parent=Msa1376540-mRNA-1
tig0019825 exon 60798 61438 60798 ID=Msa1376540-mRNA-1:exon:5224;Parent=Msa1376540-mRNA-1
tig0019825 five_prime_UTR 61347 61438 61347 ID=Msa1376540-mRNA-1:five_prime_utr;Parent=Msa1376540-mRNA-1
tig0019825 CDS 60798 61346 60798 ID=Msa1376540-mRNA-1:cds;Parent=Msa1376540-mRNA-1
tig0019825 CDS 60597 60716 60597 ID=Msa1376540-mRNA-1:cds;Parent=Msa1376540-mRNA-1
tig0019825 CDS 60493 60507 60493 ID=Msa1376540-mRNA-1:cds;Parent=Msa1376540-mRNA-1
tig0019825 three_prime_UTR 60182 60492 60182 ID=Msa1376540-mRNA-1:three_prime_utr;Parent=Msa1376540-mRNA-1
Gene Sequence

>Msa1376540

ATGCCCAAAAAGATGGGTGTGAATTCTAAGGCCGAGGCGGCGAGGGCCCGCAAGAGCGCCGCGGAAACGGAGAAAAAGGATCGGGAGACTCGAGAGAAGGAGGAGCAGTACTGGCGAGAGGCAGAGAGTTCAAAGCCACGCGCCGCCAAGAAGAGGGAGGAAGAGGCGGATAAGAAGGCGGAGGCGGCGGCACGACGAGCGGAGGTGCGTCGATTGGCGGAGCTGGAGGAGAAAGAGCTGGAGAAGATGATTAAAAAGCCTGACAAGAAGGCAAACAGGGTGTCTATCCCTGTACCGAAGGTGACGGAGGCGGAGCTGAGGAAGCGGCGAGAGGAGGAGCAAGCTATGGCACAGAAGAAGGCGGAGGAAGCGAAGAAGAGGACTGCTGGGGAGGAGGAGTACGAGAGAGTGGTGCTTGTGTCGAATACGAATCGTGATGATTCAATTATCGAAGCCAGCACTCTTGACGAAGCCATCGCTCAGATGAGTATCGATAACAACTTACCTCCCGACAGGCATCCAGAGAGGCGCCTCAAGGCTTCCTTCAAGGCATTTGAAGAAGCTGAACTACCTAAGTTGAAGCAAGAGAAACCAGGGCTTACATACACACAGTACAAGGACATGATTTGGAAGCTTTGGAAGAAATCTCCTGACAATCCTCTTAATCAAATTGCTGCCGAGTGA
Protein sequence

>Msa1376540

MPKKMGVNSKAEAARARKSAAETEKKDRETREKEEQYWREAESSKPRAAKKREEEADKKAEAAARRAEVRRLAELEEKELEKMIKKPDKKANRVSIPVPKVTEAELRKRREEEQAMAQKKAEEAKKRTAGEEEYERVVLVSNTNRDDSIIEASTLDEAIAQMSIDNNLPPDRHPERRLKASFKAFEEAELPKLKQEKPGLTYTQYKDMIWKLWKKSPDNPLNQIAAE*