AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0382520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0382520 MtrunA17_Chr3g0137781 96.178 157 6 0 12 168 21 177 2.15e-112 317
Msa0382520 MtrunA17_Chr4g0003351 50.943 159 72 4 11 168 6 159 7.67e-52 162
Msa0382520 MtrunA17_Chr4g0063831 50.943 159 69 5 13 167 18 171 5.74e-45 145
Msa0382520 MtrunA17_Chr1g0149341 55.714 140 60 2 30 168 3 141 1.55e-34 118
Msa0382520 MtrunA17_Chr1g0199151 51.515 66 31 1 104 168 21 86 1.19e-19 78.6
Msa0382520 MtrunA17_Chr7g0264721 37.500 80 46 2 91 168 28 105 1.73e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0382520 AT5G25360.3 55.128 156 64 3 13 168 67 216 8.24e-53 167
Msa0382520 AT5G25360.2 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa0382520 AT5G25360.1 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa0382520 AT4G32342.1 49.091 165 66 5 7 167 10 160 5.10e-45 145
Msa0382520 AT4G32342.2 49.091 165 66 5 7 167 48 198 1.59e-44 145
Msa0382520 AT1G15350.1 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa0382520 AT1G15350.2 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa0382520 AT3G15770.2 53.719 121 51 3 48 167 44 160 2.52e-36 124
Msa0382520 AT3G15770.1 53.719 121 51 3 48 167 45 161 2.77e-36 124
Msa0382520 AT1G15350.6 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0382520 AT1G15350.3 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0382520 AT1G15350.5 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0382520 AT1G15350.4 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0382520 AT3G54880.1 41.228 114 62 2 58 168 1 112 9.60e-24 90.1
Msa0382520 AT5G03440.2 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa0382520 AT5G03440.3 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa0382520 AT5G03440.1 42.857 84 48 0 85 168 15 98 7.61e-20 79.7

Find 89 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTTATTTACCTTATTT+TGG 0.062991 3_1:+85159115 None:intergenic
TTCATCCTCTTAATGTTTAT+TGG 0.159120 3_1:-85157847 Msa0382520:three_prime_UTR
TTGCCATGATTGACAAATTC+TGG 0.203050 3_1:+85159490 None:intergenic
TTGACTTTCTTGTTGATATA+TGG 0.216717 3_1:-85157952 Msa0382520:CDS
CAACCCATTGTTGCCTTGTT+TGG 0.223693 3_1:+85159177 None:intergenic
GACACTTGTATTTATGATCT+TGG 0.275845 3_1:-85157530 Msa0382520:three_prime_UTR
GGGCGAGGCCGATGCATCTA+AGG 0.287521 3_1:-85159692 Msa0382520:CDS
AAATGGATGACATGCTTCTC+TGG 0.301324 3_1:+85159569 None:intergenic
TGTTGTCGTTGTTCATTGTT+TGG 0.311681 3_1:+85159653 None:intergenic
AACAATGGGTTGGAAATAAA+AGG 0.312465 3_1:-85159166 Msa0382520:CDS
AGTGAAAACATAACACCTAT+TGG 0.318943 3_1:+85157558 None:intergenic
GGTTGTGTTAATCATGCTTC+TGG 0.347483 3_1:-85159782 None:intergenic
GAGCAGCAGTGCTATTGAAT+TGG 0.355690 3_1:-85159611 Msa0382520:CDS
TTGGGTTCCCCAACTTGTTT+CGG 0.356307 3_1:+85159134 None:intergenic
TATGATTGAACTGGCAAAAC+AGG 0.376802 3_1:-85157914 Msa0382520:exon
AACCAACTTAATAGAAAATC+AGG 0.378292 3_1:+85157725 None:intergenic
TGACTTTCTTGTTGATATAT+GGG 0.383956 3_1:-85157951 Msa0382520:CDS
AAGTTGGGGAACCCAAAATA+AGG 0.384880 3_1:-85159127 Msa0382520:intron
TGTTGATATATGGGAATTAG+AGG 0.391346 3_1:-85157942 Msa0382520:CDS
ACTATCCAATAAACATTAAG+AGG 0.394734 3_1:+85157842 None:intergenic
CCCAACTTGTTTCGGACGCC+CGG 0.401219 3_1:+85159142 None:intergenic
CCTCTGCTCTGTTGCAAAGC+AGG 0.407496 3_1:+85159754 None:intergenic
TCTCCAGAATTTGTCAATCA+TGG 0.416899 3_1:-85159493 Msa0382520:intron
CCGGGCGTCCGAAACAAGTT+GGG 0.420971 3_1:-85159142 Msa0382520:CDS
CAACAGAGCAGAGGTTGTCT+TGG 0.421310 3_1:-85159745 Msa0382520:CDS
CTGGAAGGAAATGTTAATCT+TGG 0.422072 3_1:+85157497 None:intergenic
GCCACCTACGAGAGTCTACT+TGG 0.426114 3_1:-85158113 Msa0382520:CDS
AAAAGACTTAGTACTTACTT+TGG 0.426312 3_1:+85157683 None:intergenic
GTGAAAACATAACACCTATT+GGG 0.432741 3_1:+85157559 None:intergenic
GTTGTCGTTGTTCATTGTTT+GGG 0.444475 3_1:+85159654 None:intergenic
TGGGTTGGAAATAAAAGGTC+CGG 0.452762 3_1:-85159161 Msa0382520:CDS
GGTGATCTCCAAGTTGGAAT+GGG 0.452928 3_1:-85157184 Msa0382520:three_prime_UTR
ATGAAAGAACTTACTCTAAG+AGG 0.460791 3_1:+85158062 None:intergenic
AGGATGGTTTGACACGGAAA+TGG 0.461819 3_1:+85159552 None:intergenic
AGAGGAATGCGCTCAGGAAA+AGG 0.472233 3_1:+85158080 None:intergenic
GATCAAATAGTCTGCAATCC+AGG 0.473644 3_1:-85157243 Msa0382520:three_prime_UTR
TGAATGCCATCACTACTTTG+AGG 0.479975 3_1:+85159523 None:intergenic
GAGGGCATGTATGATTGAAC+TGG 0.485460 3_1:-85157923 Msa0382520:exon
GATGATTCCTACGGCAGCAC+TGG 0.489025 3_1:+85157478 None:intergenic
TCCGGGCGTCCGAAACAAGT+TGG 0.490712 3_1:-85159143 Msa0382520:CDS
TTATTAATAACTGACCCAAT+AGG 0.491022 3_1:-85157573 Msa0382520:three_prime_UTR
ATCATCTCATACTGTGACAT+TGG 0.493232 3_1:-85157461 Msa0382520:three_prime_UTR
TGGTGATCTCCAAGTTGGAA+TGG 0.502647 3_1:-85157185 Msa0382520:three_prime_UTR
GAACCAAACAAGGCAACAAT+GGG 0.503294 3_1:-85159180 Msa0382520:CDS
CTTAGTGGTGATCTCCAAGT+TGG 0.503397 3_1:-85157190 Msa0382520:three_prime_UTR
TTCTTCTGTGGAACCAAACA+AGG 0.508337 3_1:-85159190 Msa0382520:CDS
AGTACTTACTTTGGTGCAGA+TGG 0.516361 3_1:+85157692 None:intergenic
TCTGCAATCCAGGACTATAG+AGG 0.524169 3_1:-85157233 Msa0382520:three_prime_UTR
CATTGGTGAGGCTTTGATGA+AGG 0.524390 3_1:-85157444 Msa0382520:three_prime_UTR
ACTCTAAGAGGAATGCGCTC+AGG 0.532544 3_1:+85158074 None:intergenic
TTCCACAACTAAGGCAGTTG+CGG 0.533016 3_1:-85157360 Msa0382520:three_prime_UTR
GAATTGATCCTCTATAGTCC+TGG 0.533065 3_1:+85157225 None:intergenic
TACTTTGAGGATCAGAAGGA+TGG 0.543246 3_1:+85159536 None:intergenic
GCTCCCGCCCCATTCCAACT+TGG 0.549677 3_1:+85157176 None:intergenic
GGAACCAAACAAGGCAACAA+TGG 0.556597 3_1:-85159181 Msa0382520:CDS
AATGGATGACATGCTTCTCT+GGG 0.557186 3_1:+85159570 None:intergenic
ATCCGCAACTGCCTTAGTTG+TGG 0.558439 3_1:+85157358 None:intergenic
GGCGAGGCCGATGCATCTAA+GGG 0.563203 3_1:-85159691 Msa0382520:CDS
TCTCCAAGTTGGAATGGGGC+GGG 0.563451 3_1:-85157179 Msa0382520:three_prime_UTR
TGTATGCAGGTCTTCTTCTG+TGG 0.571627 3_1:-85159202 Msa0382520:intron
TCCTCTCCTGCAAAATCTAG+AGG 0.574443 3_1:+85157881 None:intergenic
CCTCTCCTGCAAAATCTAGA+GGG 0.578403 3_1:+85157882 None:intergenic
TCTGATCCTCAAAGTAGTGA+TGG 0.580218 3_1:-85159529 Msa0382520:CDS
ATTCCTACGGCAGCACTGGA+AGG 0.586917 3_1:+85157482 None:intergenic
CAAACAAGGCAACAATGGGT+TGG 0.588867 3_1:-85159176 Msa0382520:CDS
GGGTTGGAAATAAAAGGTCC+GGG 0.591087 3_1:-85159160 Msa0382520:CDS
ATCTCCAAGTTGGAATGGGG+CGG 0.592965 3_1:-85157180 Msa0382520:three_prime_UTR
GTTGATATATGGGAATTAGA+GGG 0.593196 3_1:-85157941 Msa0382520:CDS
TCGCCCATTAATAAAACAAG+AGG 0.595108 3_1:+85159709 None:intergenic
TCACTACTTTGAGGATCAGA+AGG 0.595919 3_1:+85159532 None:intergenic
CAACTTGGAGATCACCACTA+AGG 0.598538 3_1:+85157191 None:intergenic
TTTGCAGGAGAGGAAAGGTG+TGG 0.612135 3_1:-85157872 Msa0382520:three_prime_UTR
ACTATTTGATCAAAATAAGA+CGG 0.613083 3_1:+85157255 None:intergenic
TTTCCTTCCAGTGCTGCCGT+AGG 0.617308 3_1:-85157485 Msa0382520:three_prime_UTR
AGTGCTATTGAATTGGATCA+TGG 0.626780 3_1:-85159604 Msa0382520:CDS
ACAGTATGAGATGATTCCTA+CGG 0.628284 3_1:+85157469 None:intergenic
CTCATACTGTGACATTGGTG+AGG 0.628731 3_1:-85157456 Msa0382520:three_prime_UTR
CCTGCTTTGCAACAGAGCAG+AGG 0.631103 3_1:-85159754 Msa0382520:CDS
AGTGAAGCTTGACTAAGCTG+AGG 0.634103 3_1:+85157624 None:intergenic
GTGATCTCCAAGTTGGAATG+GGG 0.637300 3_1:-85157183 Msa0382520:three_prime_UTR
GTGAAGCTTGACTAAGCTGA+GGG 0.640473 3_1:+85157625 None:intergenic
CTCTCCTGCAAAATCTAGAG+GGG 0.644947 3_1:+85157883 None:intergenic
CCCAAGTAGACTCTCGTAGG+TGG 0.647046 3_1:+85158112 None:intergenic
ATCAGAAGGATGGTTTGACA+CGG 0.658576 3_1:+85159546 None:intergenic
CGGGCGTCCGAAACAAGTTG+GGG 0.664193 3_1:-85159141 Msa0382520:CDS
AGTCCCAAGTAGACTCTCGT+AGG 0.676556 3_1:+85158109 None:intergenic
CCACCTACGAGAGTCTACTT+GGG 0.677939 3_1:-85158112 Msa0382520:CDS
TGAGGGATTAGTCTCCGCAG+AGG 0.727780 3_1:+85157642 None:intergenic
TGTAAATCGGATATCCTCTG+CGG 0.750038 3_1:-85157656 Msa0382520:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATTTACCTTATTTT+GGG + chr3_1:85157835-85157854 None:intergenic 10.0%
!!! TATATTTATTTACCTTATTT+TGG + chr3_1:85157836-85157855 None:intergenic 10.0%
!! AAATAAGGTAAATAAATATA+AGG - chr3_1:85157836-85157855 Msa0382520:three_prime_UTR 10.0%
!!! TTTTTTTTTCTGATTATAAT+TGG - chr3_1:85158742-85158761 Msa0382520:intron 10.0%
!!! TTTTTTTTCTGATTATAATT+GGG - chr3_1:85158743-85158762 Msa0382520:intron 10.0%
!! AAAGAAAATATACAAATAGA+GGG + chr3_1:85157518-85157537 None:intergenic 15.0%
!! AAAAGAAAATATACAAATAG+AGG + chr3_1:85157519-85157538 None:intergenic 15.0%
!!! ATTTTTTTCTTTTATCTTGA+TGG - chr3_1:85157541-85157560 Msa0382520:three_prime_UTR 15.0%
!! CAAAATATAATAAAAAGACA+AGG + chr3_1:85157685-85157704 None:intergenic 15.0%
!!! AAAACCTCTTGTTTTATTAA+TGG - chr3_1:85157235-85157254 Msa0382520:three_prime_UTR 20.0%
!!! AAACCTCTTGTTTTATTAAT+GGG - chr3_1:85157236-85157255 Msa0382520:three_prime_UTR 20.0%
!! AAGAAAATATACAAATAGAG+GGG + chr3_1:85157517-85157536 None:intergenic 20.0%
!! ATTATTACAAGCAATTGATA+AGG + chr3_1:85157714-85157733 None:intergenic 20.0%
!! TTATCAATTGCTTGTAATAA+TGG - chr3_1:85157713-85157732 Msa0382520:three_prime_UTR 20.0%
!! TAAAAAATGTATGAGTGATA+AGG - chr3_1:85158510-85158529 Msa0382520:intron 20.0%
!!! TTTTGTCAAGTTCTAATTTT+CGG - chr3_1:85158791-85158810 Msa0382520:intron 20.0%
!!! TTGTTTTTATTTTTTCTGTG+TGG - chr3_1:85159153-85159172 Msa0382520:CDS 20.0%
!! ACTATTTGATCAAAATAAGA+CGG + chr3_1:85159696-85159715 None:intergenic 20.0%
!!! ACAGATCTGAAGATTTTTTT+TGG - chr3_1:85157315-85157334 Msa0382520:three_prime_UTR 25.0%
!! TTAACTTTAAGAACCATGAA+CGG + chr3_1:85157595-85157614 None:intergenic 25.0%
!!! CTTTCTTCTATCGATTTTAT+AGG - chr3_1:85157655-85157674 Msa0382520:three_prime_UTR 25.0%
! CTTTGTATTACAACATCTAT+CGG + chr3_1:85158162-85158181 None:intergenic 25.0%
! AATACAAAGAGTAGATAAGA+TGG - chr3_1:85158173-85158192 Msa0382520:intron 25.0%
! AATCTTTCCTAATAGTTTCT+AGG + chr3_1:85158231-85158250 None:intergenic 25.0%
! AACTATTAGGAAAGATTTAG+AGG - chr3_1:85158234-85158253 Msa0382520:intron 25.0%
! GGAAAAAAATGTACAAACAA+GGG + chr3_1:85158372-85158391 None:intergenic 25.0%
! AGGAAAAAAATGTACAAACA+AGG + chr3_1:85158373-85158392 None:intergenic 25.0%
! TGGTCTAAACAATGAATAAA+AGG + chr3_1:85158469-85158488 None:intergenic 25.0%
!! CTTGAAGCAAAAGATTTTTA+TGG + chr3_1:85158489-85158508 None:intergenic 25.0%
! AAGTAGAGAAATTAATTGAG+AGG - chr3_1:85158553-85158572 Msa0382520:intron 25.0%
!! TTTTCCAAAGATAGAATCAT+GGG + chr3_1:85158677-85158696 None:intergenic 25.0%
!! ATTTTCCAAAGATAGAATCA+TGG + chr3_1:85158678-85158697 None:intergenic 25.0%
!! TTCTTTATCTACTCATTTTC+TGG - chr3_1:85158919-85158938 Msa0382520:intron 25.0%
! AATGATATTACCATCAGTTA+CGG + chr3_1:85158961-85158980 None:intergenic 25.0%
! TTGACTTTCTTGTTGATATA+TGG - chr3_1:85158996-85159015 Msa0382520:intron 25.0%
! TGACTTTCTTGTTGATATAT+GGG - chr3_1:85158997-85159016 Msa0382520:intron 25.0%
! TTCATCCTCTTAATGTTTAT+TGG - chr3_1:85159101-85159120 Msa0382520:intron 25.0%
! ACTATCCAATAAACATTAAG+AGG + chr3_1:85159109-85159128 None:intergenic 25.0%
!!! TTTTTATTTTTTCTGTGTGG+AGG - chr3_1:85159156-85159175 Msa0382520:CDS 25.0%
!! TTCCTGATTTTCTATTAAGT+TGG - chr3_1:85159221-85159240 Msa0382520:intron 25.0%
! AACCAACTTAATAGAAAATC+AGG + chr3_1:85159226-85159245 None:intergenic 25.0%
! AAAAGACTTAGTACTTACTT+TGG + chr3_1:85159268-85159287 None:intergenic 25.0%
!! AAGTCTTTTTGTGTGTAAAT+CGG - chr3_1:85159279-85159298 Msa0382520:intron 25.0%
! TTATTAATAACTGACCCAAT+AGG - chr3_1:85159375-85159394 Msa0382520:intron 25.0%
! GGAATCGCTTTAAATATATG+TGG - chr3_1:85157562-85157581 Msa0382520:three_prime_UTR 30.0%
TTACAAGCAATTGATAAGGA+TGG + chr3_1:85157710-85157729 None:intergenic 30.0%
!! AACAATGGGTTGGAAATAAA+AGG - chr3_1:85157782-85157801 Msa0382520:three_prime_UTR 30.0%
TAGAAAGGATGGTAGAAAAT+AGG - chr3_1:85158106-85158125 Msa0382520:CDS 30.0%
AGTAAGACCTAGAAACTATT+AGG - chr3_1:85158221-85158240 Msa0382520:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - chr3_1:85158246-85158265 Msa0382520:intron 30.0%
GATTTAGAGGTTAATGAGTT+GGG - chr3_1:85158247-85158266 Msa0382520:intron 30.0%
GTTATATCCGAAACCATATT+TGG + chr3_1:85158274-85158293 None:intergenic 30.0%
TGGTTTCGGATATAACATTA+TGG - chr3_1:85158278-85158297 Msa0382520:intron 30.0%
TAGAGAAATTAATTGAGAGG+TGG - chr3_1:85158556-85158575 Msa0382520:intron 30.0%
GAAATTAGGAGACGTTAATT+AGG + chr3_1:85158655-85158674 None:intergenic 30.0%
AGATAGAATCATGGGAAATT+AGG + chr3_1:85158669-85158688 None:intergenic 30.0%
ATTTCCCATGATTCTATCTT+TGG - chr3_1:85158670-85158689 Msa0382520:intron 30.0%
ATGAAAGAACTTACTCTAAG+AGG + chr3_1:85158889-85158908 None:intergenic 30.0%
TGTTGATATATGGGAATTAG+AGG - chr3_1:85159006-85159025 Msa0382520:intron 30.0%
GTTGATATATGGGAATTAGA+GGG - chr3_1:85159007-85159026 Msa0382520:intron 30.0%
!!! TTTTTTAAATAGCAGCGCTT+AGG + chr3_1:85159350-85159369 None:intergenic 30.0%
GTGAAAACATAACACCTATT+GGG + chr3_1:85159392-85159411 None:intergenic 30.0%
AGTGAAAACATAACACCTAT+TGG + chr3_1:85159393-85159412 None:intergenic 30.0%
!! AATAGGTGTTATGTTTTCAC+TGG - chr3_1:85159392-85159411 Msa0382520:intron 30.0%
GACACTTGTATTTATGATCT+TGG - chr3_1:85159418-85159437 Msa0382520:intron 30.0%
!!! GTGTTTTTTTTCCACAACTA+AGG - chr3_1:85159579-85159598 Msa0382520:CDS 30.0%
!!! TTCTTTGTTTTCTACCTTAG+TGG - chr3_1:85159743-85159762 Msa0382520:CDS 30.0%
TCGCCCATTAATAAAACAAG+AGG + chr3_1:85157242-85157261 None:intergenic 35.0%
!!! TCTTGTTTTATTAATGGGCG+AGG - chr3_1:85157241-85157260 Msa0382520:three_prime_UTR 35.0%
! TTTTAGTCCCTTAGATGCAT+CGG + chr3_1:85157267-85157286 None:intergenic 35.0%
GTTGTCGTTGTTCATTGTTT+GGG + chr3_1:85157297-85157316 None:intergenic 35.0%
TGTTGTCGTTGTTCATTGTT+TGG + chr3_1:85157298-85157317 None:intergenic 35.0%
! AGTGCTATTGAATTGGATCA+TGG - chr3_1:85157344-85157363 Msa0382520:three_prime_UTR 35.0%
TCTCCAGAATTTGTCAATCA+TGG - chr3_1:85157455-85157474 Msa0382520:three_prime_UTR 35.0%
TTGCCATGATTGACAAATTC+TGG + chr3_1:85157461-85157480 None:intergenic 35.0%
AGGGGTGATAATAGATAAAC+TGG + chr3_1:85157499-85157518 None:intergenic 35.0%
!! TGGTAAGTTTTGATGTATGC+AGG - chr3_1:85157733-85157752 Msa0382520:three_prime_UTR 35.0%
TATAAGGTTTCTGCATGAAG+AGG - chr3_1:85157852-85157871 Msa0382520:three_prime_UTR 35.0%
CAAGGGCAAAAATGATCAAA+TGG + chr3_1:85157928-85157947 None:intergenic 35.0%
TTGGTAAGACTAGACTAGAT+AGG - chr3_1:85158037-85158056 Msa0382520:intron 35.0%
AGACTAGACTAGATAGGATT+AGG - chr3_1:85158043-85158062 Msa0382520:intron 35.0%
ACACAATTAGAGAGAGAGTT+GGG - chr3_1:85158070-85158089 Msa0382520:intron 35.0%
TCTACCATCCTTTCTACTAT+AGG + chr3_1:85158102-85158121 None:intergenic 35.0%
AAAATAGGCTTAGGTGGTTT+GGG - chr3_1:85158121-85158140 Msa0382520:CDS 35.0%
ACAAAGAGTAGATAAGATGG+AGG - chr3_1:85158176-85158195 Msa0382520:intron 35.0%
! TAATGAGTTGGGTCCAAATA+TGG - chr3_1:85158258-85158277 Msa0382520:intron 35.0%
TAGTCAAAGAGAATAAGCAC+GGG + chr3_1:85158401-85158420 None:intergenic 35.0%
CTAGTCAAAGAGAATAAGCA+CGG + chr3_1:85158402-85158421 None:intergenic 35.0%
AGAAATTAATTGAGAGGTGG+AGG - chr3_1:85158559-85158578 Msa0382520:intron 35.0%
GAAATTAATTGAGAGGTGGA+GGG - chr3_1:85158560-85158579 Msa0382520:intron 35.0%
TAGGAGACGTTAATTAGGAT+AGG + chr3_1:85158650-85158669 None:intergenic 35.0%
TGATTATAATTGGGACCAGA+AGG - chr3_1:85158752-85158771 Msa0382520:intron 35.0%
! ATCTTAAAAAGTACGCCTTC+TGG + chr3_1:85158770-85158789 None:intergenic 35.0%
TTCGGATTTATGTTCCTAGT+TGG - chr3_1:85158809-85158828 Msa0382520:intron 35.0%
ATTACCATCAGTTACGGTTA+GGG + chr3_1:85158955-85158974 None:intergenic 35.0%
TATTACCATCAGTTACGGTT+AGG + chr3_1:85158956-85158975 None:intergenic 35.0%
ATGGTAATATCATTCGTGAC+AGG - chr3_1:85158967-85158986 Msa0382520:intron 35.0%
TATGATTGAACTGGCAAAAC+AGG - chr3_1:85159034-85159053 Msa0382520:intron 35.0%
CTGGAAGGAAATGTTAATCT+TGG + chr3_1:85159454-85159473 None:intergenic 35.0%
ACAGTATGAGATGATTCCTA+CGG + chr3_1:85159482-85159501 None:intergenic 35.0%
ATCATCTCATACTGTGACAT+TGG - chr3_1:85159487-85159506 Msa0382520:intron 35.0%
! CACAAAGTTGTTCTGTTTTC+AGG - chr3_1:85159532-85159551 Msa0382520:CDS 35.0%
AATGGATGACATGCTTCTCT+GGG + chr3_1:85157381-85157400 None:intergenic 40.0%
AAATGGATGACATGCTTCTC+TGG + chr3_1:85157382-85157401 None:intergenic 40.0%
ATCAGAAGGATGGTTTGACA+CGG + chr3_1:85157405-85157424 None:intergenic 40.0%
! TACTTTGAGGATCAGAAGGA+TGG + chr3_1:85157415-85157434 None:intergenic 40.0%
! TCACTACTTTGAGGATCAGA+AGG + chr3_1:85157419-85157438 None:intergenic 40.0%
TCTGATCCTCAAAGTAGTGA+TGG - chr3_1:85157419-85157438 Msa0382520:three_prime_UTR 40.0%
TGAATGCCATCACTACTTTG+AGG + chr3_1:85157428-85157447 None:intergenic 40.0%
TTCTTCTGTGGAACCAAACA+AGG - chr3_1:85157758-85157777 Msa0382520:three_prime_UTR 40.0%
GAACCAAACAAGGCAACAAT+GGG - chr3_1:85157768-85157787 Msa0382520:three_prime_UTR 40.0%
! TGGGTTGGAAATAAAAGGTC+CGG - chr3_1:85157787-85157806 Msa0382520:three_prime_UTR 40.0%
AAGTTGGGGAACCCAAAATA+AGG - chr3_1:85157821-85157840 Msa0382520:three_prime_UTR 40.0%
! TTTTGCCCTTGCATGTTGTA+TGG - chr3_1:85157937-85157956 Msa0382520:CDS 40.0%
TGGGCGGTTAAAAATCAACA+CGG - chr3_1:85157970-85157989 Msa0382520:intron 40.0%
CGGAAATCAAGTAAGTGTAG+CGG - chr3_1:85157990-85158009 Msa0382520:intron 40.0%
GACACAATTAGAGAGAGAGT+TGG - chr3_1:85158069-85158088 Msa0382520:intron 40.0%
CACAATTAGAGAGAGAGTTG+GGG - chr3_1:85158071-85158090 Msa0382520:intron 40.0%
AGCACCTATAGTAGAAAGGA+TGG - chr3_1:85158095-85158114 Msa0382520:CDS 40.0%
GGATGGTAGAAAATAGGCTT+AGG - chr3_1:85158112-85158131 Msa0382520:CDS 40.0%
TGGTAGAAAATAGGCTTAGG+TGG - chr3_1:85158115-85158134 Msa0382520:CDS 40.0%
GAAAATAGGCTTAGGTGGTT+TGG - chr3_1:85158120-85158139 Msa0382520:CDS 40.0%
GTTGGGTCCAAATATGGTTT+CGG - chr3_1:85158264-85158283 Msa0382520:intron 40.0%
CGTGCTTATTCTCTTTGACT+AGG - chr3_1:85158400-85158419 Msa0382520:intron 40.0%
GTTAATTAGGATAGGAGGCA+GGG + chr3_1:85158642-85158661 None:intergenic 40.0%
GAGACGTTAATTAGGATAGG+AGG + chr3_1:85158647-85158666 None:intergenic 40.0%
! TATTCCCCTCTAGATTTTGC+AGG - chr3_1:85159061-85159080 Msa0382520:intron 40.0%
! TAGATTTTGCAGGAGAGGAA+AGG - chr3_1:85159071-85159090 Msa0382520:intron 40.0%
! AGTACTTACTTTGGTGCAGA+TGG + chr3_1:85159259-85159278 None:intergenic 40.0%
TGTAAATCGGATATCCTCTG+CGG - chr3_1:85159292-85159311 Msa0382520:intron 40.0%
!! GGTGTTATGTTTTCACTGGA+TGG - chr3_1:85159396-85159415 Msa0382520:intron 40.0%
GATCAAATAGTCTGCAATCC+AGG - chr3_1:85159705-85159724 Msa0382520:CDS 40.0%
GAATTGATCCTCTATAGTCC+TGG + chr3_1:85159726-85159745 None:intergenic 40.0%
! GAGCAGCAGTGCTATTGAAT+TGG - chr3_1:85157337-85157356 Msa0382520:three_prime_UTR 45.0%
AGGATGGTTTGACACGGAAA+TGG + chr3_1:85157399-85157418 None:intergenic 45.0%
! ATGTGGTTGCTTTCCGTTCA+TGG - chr3_1:85157579-85157598 Msa0382520:three_prime_UTR 45.0%
TGTATGCAGGTCTTCTTCTG+TGG - chr3_1:85157746-85157765 Msa0382520:three_prime_UTR 45.0%
GGAACCAAACAAGGCAACAA+TGG - chr3_1:85157767-85157786 Msa0382520:three_prime_UTR 45.0%
! CAACCCATTGTTGCCTTGTT+TGG + chr3_1:85157774-85157793 None:intergenic 45.0%
CAAACAAGGCAACAATGGGT+TGG - chr3_1:85157772-85157791 Msa0382520:three_prime_UTR 45.0%
GGGTTGGAAATAAAAGGTCC+GGG - chr3_1:85157788-85157807 Msa0382520:three_prime_UTR 45.0%
! TTGGGTTCCCCAACTTGTTT+CGG + chr3_1:85157817-85157836 None:intergenic 45.0%
GTTTCTGCATGAAGAGGCTT+AGG - chr3_1:85157858-85157877 Msa0382520:three_prime_UTR 45.0%
ATGAAGAGGCTTAGGTGGTT+TGG - chr3_1:85157866-85157885 Msa0382520:three_prime_UTR 45.0%
!! TAGGTGGTTTGGTCATTACC+AGG - chr3_1:85157877-85157896 Msa0382520:three_prime_UTR 45.0%
!! AGGTGGTTTGGTCATTACCA+GGG - chr3_1:85157878-85157897 Msa0382520:three_prime_UTR 45.0%
ATGCAATACTGTACTTGCCC+TGG + chr3_1:85157898-85157917 None:intergenic 45.0%
CCGTACCATACAACATGCAA+GGG + chr3_1:85157945-85157964 None:intergenic 45.0%
CCCTTGCATGTTGTATGGTA+CGG - chr3_1:85157942-85157961 Msa0382520:CDS 45.0%
TCCGTACCATACAACATGCA+AGG + chr3_1:85157946-85157965 None:intergenic 45.0%
TGTTGTATGGTACGGAGTGT+TGG - chr3_1:85157950-85157969 Msa0382520:CDS 45.0%
! GTTGTATGGTACGGAGTGTT+GGG - chr3_1:85157951-85157970 Msa0382520:CDS 45.0%
! AAGTAAGTGTAGCGGAGATG+AGG - chr3_1:85157998-85158017 Msa0382520:intron 45.0%
AGGATGTTGCGTTGGATGAT+TGG - chr3_1:85158018-85158037 Msa0382520:intron 45.0%
GGGTAGCACCTATAGTAGAA+AGG - chr3_1:85158091-85158110 Msa0382520:CDS 45.0%
GAGGAGAGTCTGATATGAAG+AGG - chr3_1:85158195-85158214 Msa0382520:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + chr3_1:85158327-85158346 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + chr3_1:85158328-85158347 None:intergenic 45.0%
CGTTAATTAGGATAGGAGGC+AGG + chr3_1:85158643-85158662 None:intergenic 45.0%
! GAGGGCATGTATGATTGAAC+TGG - chr3_1:85159025-85159044 Msa0382520:intron 45.0%
CTCTCCTGCAAAATCTAGAG+GGG + chr3_1:85159068-85159087 None:intergenic 45.0%
CCTCTCCTGCAAAATCTAGA+GGG + chr3_1:85159069-85159088 None:intergenic 45.0%
TCCTCTCCTGCAAAATCTAG+AGG + chr3_1:85159070-85159089 None:intergenic 45.0%
GTGAAGCTTGACTAAGCTGA+GGG + chr3_1:85159326-85159345 None:intergenic 45.0%
AGTGAAGCTTGACTAAGCTG+AGG + chr3_1:85159327-85159346 None:intergenic 45.0%
CTCATACTGTGACATTGGTG+AGG - chr3_1:85159492-85159511 Msa0382520:intron 45.0%
! CATTGGTGAGGCTTTGATGA+AGG - chr3_1:85159504-85159523 Msa0382520:CDS 45.0%
!!! GTTGTTCTGTTTTCAGGTGC+AGG - chr3_1:85159538-85159557 Msa0382520:CDS 45.0%
TTCCACAACTAAGGCAGTTG+CGG - chr3_1:85159588-85159607 Msa0382520:CDS 45.0%
TCTGCAATCCAGGACTATAG+AGG - chr3_1:85159715-85159734 Msa0382520:CDS 45.0%
CAACTTGGAGATCACCACTA+AGG + chr3_1:85159760-85159779 None:intergenic 45.0%
CTTAGTGGTGATCTCCAAGT+TGG - chr3_1:85159758-85159777 Msa0382520:CDS 45.0%
! TGGTGATCTCCAAGTTGGAA+TGG - chr3_1:85159763-85159782 Msa0382520:CDS 45.0%
! GGTGATCTCCAAGTTGGAAT+GGG - chr3_1:85159764-85159783 Msa0382520:CDS 45.0%
! GTGATCTCCAAGTTGGAATG+GGG - chr3_1:85159765-85159784 Msa0382520:CDS 45.0%
CAACAGAGCAGAGGTTGTCT+TGG - chr3_1:85157203-85157222 Msa0382520:three_prime_UTR 50.0%
TCTGCATGAAGAGGCTTAGG+TGG - chr3_1:85157861-85157880 Msa0382520:three_prime_UTR 50.0%
CTTATCCCACTAAGTGGGGT+CGG + chr3_1:85158323-85158342 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + chr3_1:85158329-85158348 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - chr3_1:85158326-85158345 Msa0382520:intron 50.0%
GAGAATAAGCACGGGCAGTA+AGG + chr3_1:85158393-85158412 None:intergenic 50.0%
GAGGTGGAGGGAGTTAAACT+TGG - chr3_1:85158572-85158591 Msa0382520:intron 50.0%
CCACCTACGAGAGTCTACTT+GGG - chr3_1:85158836-85158855 Msa0382520:intron 50.0%
AGTCCCAAGTAGACTCTCGT+AGG + chr3_1:85158842-85158861 None:intergenic 50.0%
AGAGGAATGCGCTCAGGAAA+AGG + chr3_1:85158871-85158890 None:intergenic 50.0%
! ACTCTAAGAGGAATGCGCTC+AGG + chr3_1:85158877-85158896 None:intergenic 50.0%
TGCTCCCTAACCGTAACTGA+TGG - chr3_1:85158948-85158967 Msa0382520:intron 50.0%
! CCCTCTAGATTTTGCAGGAG+AGG - chr3_1:85159066-85159085 Msa0382520:intron 50.0%
TTTGCAGGAGAGGAAAGGTG+TGG - chr3_1:85159076-85159095 Msa0382520:intron 50.0%
ATCCGCAACTGCCTTAGTTG+TGG + chr3_1:85159593-85159612 None:intergenic 50.0%
! ATCTCCAAGTTGGAATGGGG+CGG - chr3_1:85159768-85159787 Msa0382520:CDS 50.0%
CCTCTGCTCTGTTGCAAAGC+AGG + chr3_1:85157197-85157216 None:intergenic 55.0%
CCTGCTTTGCAACAGAGCAG+AGG - chr3_1:85157194-85157213 Msa0382520:three_prime_UTR 55.0%
!! GTATGGTACGGAGTGTTGGG+CGG - chr3_1:85157954-85157973 Msa0382520:CDS 55.0%
CGGAGATGAGGATGTTGCGT+TGG - chr3_1:85158010-85158029 Msa0382520:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr3_1:85158315-85158334 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr3_1:85158314-85158333 Msa0382520:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr3_1:85158315-85158334 Msa0382520:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG - chr3_1:85158321-85158340 Msa0382520:intron 55.0%
TCGTAGGTGGCGTTCCAACT+AGG + chr3_1:85158826-85158845 None:intergenic 55.0%
GCCACCTACGAGAGTCTACT+TGG - chr3_1:85158835-85158854 Msa0382520:intron 55.0%
CCCAAGTAGACTCTCGTAGG+TGG + chr3_1:85158839-85158858 None:intergenic 55.0%
!! TGAGGGATTAGTCTCCGCAG+AGG + chr3_1:85159309-85159328 None:intergenic 55.0%
TTTCCTTCCAGTGCTGCCGT+AGG - chr3_1:85159463-85159482 Msa0382520:intron 55.0%
! ATTCCTACGGCAGCACTGGA+AGG + chr3_1:85159469-85159488 None:intergenic 55.0%
GATGATTCCTACGGCAGCAC+TGG + chr3_1:85159473-85159492 None:intergenic 55.0%
GGCGAGGCCGATGCATCTAA+GGG - chr3_1:85157257-85157276 Msa0382520:three_prime_UTR 60.0%
TCCGGGCGTCCGAAACAAGT+TGG - chr3_1:85157805-85157824 Msa0382520:three_prime_UTR 60.0%
! CCCAACTTGTTTCGGACGCC+CGG + chr3_1:85157809-85157828 None:intergenic 60.0%
CCGGGCGTCCGAAACAAGTT+GGG - chr3_1:85157806-85157825 Msa0382520:three_prime_UTR 60.0%
CGGGCGTCCGAAACAAGTTG+GGG - chr3_1:85157807-85157826 Msa0382520:three_prime_UTR 60.0%
GGGCGAGGCCGATGCATCTA+AGG - chr3_1:85157256-85157275 Msa0382520:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 85157179 85159791 85157179 ID=Msa0382520;Name=Msa0382520
chr3_1 mRNA 85157179 85159791 85157179 ID=Msa0382520-mRNA-1;Parent=Msa0382520;Name=Msa0382520-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|1|0.75|1|1|1|4|749|168
chr3_1 exon 85157179 85157981 85157179 ID=Msa0382520-mRNA-1:exon:22301;Parent=Msa0382520-mRNA-1
chr3_1 exon 85158076 85158142 85158076 ID=Msa0382520-mRNA-1:exon:22300;Parent=Msa0382520-mRNA-1
chr3_1 exon 85159128 85159215 85159128 ID=Msa0382520-mRNA-1:exon:22299;Parent=Msa0382520-mRNA-1
chr3_1 exon 85159494 85159791 85159494 ID=Msa0382520-mRNA-1:exon:22298;Parent=Msa0382520-mRNA-1
chr3_1 CDS 85159494 85159791 85159494 ID=Msa0382520-mRNA-1:cds;Parent=Msa0382520-mRNA-1
chr3_1 CDS 85159128 85159215 85159128 ID=Msa0382520-mRNA-1:cds;Parent=Msa0382520-mRNA-1
chr3_1 CDS 85158076 85158142 85158076 ID=Msa0382520-mRNA-1:cds;Parent=Msa0382520-mRNA-1
chr3_1 CDS 85157928 85157981 85157928 ID=Msa0382520-mRNA-1:cds;Parent=Msa0382520-mRNA-1
chr3_1 three_prime_UTR 85157179 85157927 85157179 ID=Msa0382520-mRNA-1:three_prime_utr;Parent=Msa0382520-mRNA-1
Gene Sequence

>Msa0382520

ATGCTTCTGGTTAAACCTGCTTTGCAACAGAGCAGAGGTTGTCTTGGATGTTTCTCAAAACCTCTTGTTTTATTAATGGGCGAGGCCGATGCATCTAAGGGACTAAAAACTCAAGCCCAAACAATGAACAACGACAACAGATCTGAAGATTTTTTTTGGAGCAGCAGTGCTATTGAATTGGATCATGGTGCTATTCAGTCCCAGAGAAGCATGTCATCCATTTCCGTGTCAAACCATCCTTCTGATCCTCAAAGTAGTGATGGCATTCAGACTGATTCTCCAGAATTTGTCAATCATGGTCTTCTTCTGTGGAACCAAACAAGGCAACAATGGGTTGGAAATAAAAGGTCCGGGCGTCCGAAACAAGTTGGGGAACCCAAAATAAGTTGGAACGCCACCTACGAGAGTCTACTTGGGACTAACAAGCCTTTTCCTGAGCGCATTCCTCTTAGAGAAATGATTGACTTTCTTGTTGATATATGGGAATTAGAGGGCATGTATGATTGA
Protein sequence

>Msa0382520

MLLVKPALQQSRGCLGCFSKPLVLLMGEADASKGLKTQAQTMNNDNRSEDFFWSSSAIELDHGAIQSQRSMSSISVSNHPSDPQSSDGIQTDSPEFVNHGLLLWNQTRQQWVGNKRSGRPKQVGEPKISWNATYESLLGTNKPFPERIPLREMIDFLVDIWELEGMYD*