AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0525200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0525200 MtrunA17_Chr3g0137781 96.178 157 6 0 12 168 21 177 2.15e-112 317
Msa0525200 MtrunA17_Chr4g0003351 50.943 159 72 4 11 168 6 159 7.67e-52 162
Msa0525200 MtrunA17_Chr4g0063831 50.943 159 69 5 13 167 18 171 5.74e-45 145
Msa0525200 MtrunA17_Chr1g0149341 55.714 140 60 2 30 168 3 141 1.55e-34 118
Msa0525200 MtrunA17_Chr1g0199151 51.515 66 31 1 104 168 21 86 1.19e-19 78.6
Msa0525200 MtrunA17_Chr7g0264721 37.500 80 46 2 91 168 28 105 1.73e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0525200 AT5G25360.3 55.128 156 64 3 13 168 67 216 8.24e-53 167
Msa0525200 AT5G25360.2 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa0525200 AT5G25360.1 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa0525200 AT4G32342.1 49.091 165 66 5 7 167 10 160 5.10e-45 145
Msa0525200 AT4G32342.2 49.091 165 66 5 7 167 48 198 1.59e-44 145
Msa0525200 AT1G15350.1 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa0525200 AT1G15350.2 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa0525200 AT3G15770.2 53.719 121 51 3 48 167 44 160 2.52e-36 124
Msa0525200 AT3G15770.1 53.719 121 51 3 48 167 45 161 2.77e-36 124
Msa0525200 AT1G15350.6 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0525200 AT1G15350.3 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0525200 AT1G15350.5 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0525200 AT1G15350.4 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa0525200 AT3G54880.1 41.228 114 62 2 58 168 1 112 9.60e-24 90.1
Msa0525200 AT5G03440.2 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa0525200 AT5G03440.3 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa0525200 AT5G03440.1 42.857 84 48 0 85 168 15 98 7.61e-20 79.7

Find 89 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTTATTTACCTTATTT+TGG 0.062991 3_4:+78735557 None:intergenic
TTCATCCTCTTAATGTTTAT+TGG 0.159120 3_4:-78734290 Msa0525200:three_prime_UTR
TTGCCATGATTGACAAATTC+TGG 0.203050 3_4:+78735934 None:intergenic
TTGACTTTCTTGTTGATATA+TGG 0.216717 3_4:-78734395 Msa0525200:CDS
CAACCCATTGTTGCCTTGTT+TGG 0.223693 3_4:+78735619 None:intergenic
GACACTTGTATTTATGATCT+TGG 0.275845 3_4:-78733973 Msa0525200:three_prime_UTR
GGGCGAGGCCGATGCATCTA+AGG 0.287521 3_4:-78736136 Msa0525200:CDS
AAATGGATGACATGCTTCTC+TGG 0.301324 3_4:+78736013 None:intergenic
TGTTGTCGTTGTTCATTGTT+TGG 0.311681 3_4:+78736097 None:intergenic
AACAATGGGTTGGAAATAAA+AGG 0.312465 3_4:-78735608 Msa0525200:CDS
AGTGAAAACATAACACCTAT+TGG 0.318943 3_4:+78734001 None:intergenic
GGTTGTGTTAATCATGCTTC+TGG 0.347483 3_4:-78736226 None:intergenic
GAGCAGCAGTGCTATTGAAT+TGG 0.355690 3_4:-78736055 Msa0525200:CDS
TTGGGTTCCCCAACTTGTTT+CGG 0.356307 3_4:+78735576 None:intergenic
TATGATTGAACTGGCAAAAC+AGG 0.376802 3_4:-78734357 Msa0525200:exon
AACCAACTTAATAGAAAATC+AGG 0.378292 3_4:+78734168 None:intergenic
TGACTTTCTTGTTGATATAT+GGG 0.383956 3_4:-78734394 Msa0525200:CDS
AAGTTGGGGAACCCAAAATA+AGG 0.384880 3_4:-78735569 Msa0525200:intron
TGTTGATATATGGGAATTAG+AGG 0.391346 3_4:-78734385 Msa0525200:CDS
ACTATCCAATAAACATTAAG+AGG 0.394734 3_4:+78734285 None:intergenic
CCCAACTTGTTTCGGACGCC+CGG 0.401219 3_4:+78735584 None:intergenic
CCTCTGCTCTGTTGCAAAGC+AGG 0.407496 3_4:+78736198 None:intergenic
TCTCCAGAATTTGTCAATCA+TGG 0.416899 3_4:-78735937 Msa0525200:intron
CCGGGCGTCCGAAACAAGTT+GGG 0.420971 3_4:-78735584 Msa0525200:CDS
CAACAGAGCAGAGGTTGTCT+TGG 0.421310 3_4:-78736189 Msa0525200:CDS
CTGGAAGGAAATGTTAATCT+TGG 0.422072 3_4:+78733940 None:intergenic
GCCACCTACGAGAGTCTACT+TGG 0.426114 3_4:-78734556 Msa0525200:CDS
AAAAGACTTAGTACTTACTT+TGG 0.426312 3_4:+78734126 None:intergenic
GTGAAAACATAACACCTATT+GGG 0.432741 3_4:+78734002 None:intergenic
GTTGTCGTTGTTCATTGTTT+GGG 0.444475 3_4:+78736098 None:intergenic
TGGGTTGGAAATAAAAGGTC+CGG 0.452762 3_4:-78735603 Msa0525200:CDS
GGTGATCTCCAAGTTGGAAT+GGG 0.452928 3_4:-78733627 Msa0525200:three_prime_UTR
ATGAAAGAACTTACTCTAAG+AGG 0.460791 3_4:+78734505 None:intergenic
AGGATGGTTTGACACGGAAA+TGG 0.461819 3_4:+78735996 None:intergenic
AGAGGAATGCGCTCAGGAAA+AGG 0.472233 3_4:+78734523 None:intergenic
GATCAAATAGTCTGCAATCC+AGG 0.473644 3_4:-78733686 Msa0525200:three_prime_UTR
TGAATGCCATCACTACTTTG+AGG 0.479975 3_4:+78735967 None:intergenic
GAGGGCATGTATGATTGAAC+TGG 0.485460 3_4:-78734366 Msa0525200:exon
GATGATTCCTACGGCAGCAC+TGG 0.489025 3_4:+78733921 None:intergenic
TCCGGGCGTCCGAAACAAGT+TGG 0.490712 3_4:-78735585 Msa0525200:CDS
TTATTAATAACTGACCCAAT+AGG 0.491022 3_4:-78734016 Msa0525200:three_prime_UTR
ATCATCTCATACTGTGACAT+TGG 0.493232 3_4:-78733904 Msa0525200:three_prime_UTR
TGGTGATCTCCAAGTTGGAA+TGG 0.502647 3_4:-78733628 Msa0525200:three_prime_UTR
GAACCAAACAAGGCAACAAT+GGG 0.503294 3_4:-78735622 Msa0525200:CDS
CTTAGTGGTGATCTCCAAGT+TGG 0.503397 3_4:-78733633 Msa0525200:three_prime_UTR
TTCTTCTGTGGAACCAAACA+AGG 0.508337 3_4:-78735632 Msa0525200:CDS
AGTACTTACTTTGGTGCAGA+TGG 0.516361 3_4:+78734135 None:intergenic
TCTGCAATCCAGGACTATAG+AGG 0.524169 3_4:-78733676 Msa0525200:three_prime_UTR
CATTGGTGAGGCTTTGATGA+AGG 0.524390 3_4:-78733887 Msa0525200:three_prime_UTR
ACTCTAAGAGGAATGCGCTC+AGG 0.532544 3_4:+78734517 None:intergenic
TTCCACAACTAAGGCAGTTG+CGG 0.533016 3_4:-78733803 Msa0525200:three_prime_UTR
GAATTGATCCTCTATAGTCC+TGG 0.533065 3_4:+78733668 None:intergenic
TACTTTGAGGATCAGAAGGA+TGG 0.543246 3_4:+78735980 None:intergenic
GCTCCCGCCCCATTCCAACT+TGG 0.549677 3_4:+78733619 None:intergenic
GGAACCAAACAAGGCAACAA+TGG 0.556597 3_4:-78735623 Msa0525200:CDS
AATGGATGACATGCTTCTCT+GGG 0.557186 3_4:+78736014 None:intergenic
ATCCGCAACTGCCTTAGTTG+TGG 0.558439 3_4:+78733801 None:intergenic
GGCGAGGCCGATGCATCTAA+GGG 0.563203 3_4:-78736135 Msa0525200:CDS
TCTCCAAGTTGGAATGGGGC+GGG 0.563451 3_4:-78733622 Msa0525200:three_prime_UTR
TGTATGCAGGTCTTCTTCTG+TGG 0.571627 3_4:-78735644 Msa0525200:intron
TCCTCTCCTGCAAAATCTAG+AGG 0.574443 3_4:+78734324 None:intergenic
CCTCTCCTGCAAAATCTAGA+GGG 0.578403 3_4:+78734325 None:intergenic
TCTGATCCTCAAAGTAGTGA+TGG 0.580218 3_4:-78735973 Msa0525200:CDS
ATTCCTACGGCAGCACTGGA+AGG 0.586917 3_4:+78733925 None:intergenic
CAAACAAGGCAACAATGGGT+TGG 0.588867 3_4:-78735618 Msa0525200:CDS
GGGTTGGAAATAAAAGGTCC+GGG 0.591087 3_4:-78735602 Msa0525200:CDS
ATCTCCAAGTTGGAATGGGG+CGG 0.592965 3_4:-78733623 Msa0525200:three_prime_UTR
GTTGATATATGGGAATTAGA+GGG 0.593196 3_4:-78734384 Msa0525200:CDS
TCGCCCATTAATAAAACAAG+AGG 0.595108 3_4:+78736153 None:intergenic
TCACTACTTTGAGGATCAGA+AGG 0.595919 3_4:+78735976 None:intergenic
CAACTTGGAGATCACCACTA+AGG 0.598538 3_4:+78733634 None:intergenic
TTTGCAGGAGAGGAAAGGTG+TGG 0.612135 3_4:-78734315 Msa0525200:three_prime_UTR
ACTATTTGATCAAAATAAGA+CGG 0.613083 3_4:+78733698 None:intergenic
TTTCCTTCCAGTGCTGCCGT+AGG 0.617308 3_4:-78733928 Msa0525200:three_prime_UTR
AGTGCTATTGAATTGGATCA+TGG 0.626780 3_4:-78736048 Msa0525200:CDS
ACAGTATGAGATGATTCCTA+CGG 0.628284 3_4:+78733912 None:intergenic
CTCATACTGTGACATTGGTG+AGG 0.628731 3_4:-78733899 Msa0525200:three_prime_UTR
CCTGCTTTGCAACAGAGCAG+AGG 0.631103 3_4:-78736198 Msa0525200:CDS
AGTGAAGCTTGACTAAGCTG+AGG 0.634103 3_4:+78734067 None:intergenic
GTGATCTCCAAGTTGGAATG+GGG 0.637300 3_4:-78733626 Msa0525200:three_prime_UTR
GTGAAGCTTGACTAAGCTGA+GGG 0.640473 3_4:+78734068 None:intergenic
CTCTCCTGCAAAATCTAGAG+GGG 0.644947 3_4:+78734326 None:intergenic
CCCAAGTAGACTCTCGTAGG+TGG 0.647046 3_4:+78734555 None:intergenic
ATCAGAAGGATGGTTTGACA+CGG 0.658576 3_4:+78735990 None:intergenic
CGGGCGTCCGAAACAAGTTG+GGG 0.664193 3_4:-78735583 Msa0525200:CDS
AGTCCCAAGTAGACTCTCGT+AGG 0.676556 3_4:+78734552 None:intergenic
CCACCTACGAGAGTCTACTT+GGG 0.677939 3_4:-78734555 Msa0525200:CDS
TGAGGGATTAGTCTCCGCAG+AGG 0.727780 3_4:+78734085 None:intergenic
TGTAAATCGGATATCCTCTG+CGG 0.750038 3_4:-78734099 Msa0525200:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATTTACCTTATTTT+GGG + chr3_4:78734280-78734299 None:intergenic 10.0%
!!! TATATTTATTTACCTTATTT+TGG + chr3_4:78734281-78734300 None:intergenic 10.0%
!! AAATAAGGTAAATAAATATA+AGG - chr3_4:78734281-78734300 Msa0525200:three_prime_UTR 10.0%
!!! TTTTTTTTTCTGATTATAAT+TGG - chr3_4:78735186-78735205 Msa0525200:intron 10.0%
!!! TTTTTTTTCTGATTATAATT+GGG - chr3_4:78735187-78735206 Msa0525200:intron 10.0%
!! AAAGAAAATATACAAATAGA+GGG + chr3_4:78733961-78733980 None:intergenic 15.0%
!! AAAAGAAAATATACAAATAG+AGG + chr3_4:78733962-78733981 None:intergenic 15.0%
!! CAAAATATAATAAAAAGACA+AGG + chr3_4:78734130-78734149 None:intergenic 15.0%
!!! AAAACCTCTTGTTTTATTAA+TGG - chr3_4:78733678-78733697 Msa0525200:three_prime_UTR 20.0%
!!! AAACCTCTTGTTTTATTAAT+GGG - chr3_4:78733679-78733698 Msa0525200:three_prime_UTR 20.0%
!! AAGAAAATATACAAATAGAG+GGG + chr3_4:78733960-78733979 None:intergenic 20.0%
!!! TTTTGTTTCTTTTATCTTGA+TGG - chr3_4:78733985-78734004 Msa0525200:three_prime_UTR 20.0%
!! ATTATTACAAGCAATTGATA+AGG + chr3_4:78734159-78734178 None:intergenic 20.0%
!! TTATCAATTGCTTGTAATAA+TGG - chr3_4:78734158-78734177 Msa0525200:three_prime_UTR 20.0%
!! TAAAAAATGTATGAGTGATA+AGG - chr3_4:78734955-78734974 Msa0525200:intron 20.0%
!!! TTTTGTCAAGTTCTAATTTT+CGG - chr3_4:78735235-78735254 Msa0525200:intron 20.0%
!!! TTGTTTTTATTTTTTCTGTG+TGG - chr3_4:78735597-78735616 Msa0525200:CDS 20.0%
!! ACTATTTGATCAAAATAAGA+CGG + chr3_4:78736140-78736159 None:intergenic 20.0%
!!! ACAGATCTGAAGATTTTTTT+TGG - chr3_4:78733758-78733777 Msa0525200:three_prime_UTR 25.0%
!! TTAACTTTAAGAACCATGAA+CGG + chr3_4:78734039-78734058 None:intergenic 25.0%
!!! CTTTCTTCTATCGATTTTAT+AGG - chr3_4:78734100-78734119 Msa0525200:three_prime_UTR 25.0%
! CTTTGTATTACAACATCTAT+CGG + chr3_4:78734607-78734626 None:intergenic 25.0%
! AATACAAAGAGTAGATAAGA+TGG - chr3_4:78734618-78734637 Msa0525200:intron 25.0%
! AATCTTTCCTAATAGTTTCT+AGG + chr3_4:78734676-78734695 None:intergenic 25.0%
! AACTATTAGGAAAGATTTAG+AGG - chr3_4:78734679-78734698 Msa0525200:intron 25.0%
! GGAAAAAAATGTACAAACAA+GGG + chr3_4:78734817-78734836 None:intergenic 25.0%
! AGGAAAAAAATGTACAAACA+AGG + chr3_4:78734818-78734837 None:intergenic 25.0%
! TGGTCTAAACAATGAATAAA+AGG + chr3_4:78734914-78734933 None:intergenic 25.0%
!! CTTGAAGCAAAAGATTTTTA+TGG + chr3_4:78734934-78734953 None:intergenic 25.0%
! AAGTAGAGAAATTAATTGAG+AGG - chr3_4:78734998-78735017 Msa0525200:intron 25.0%
!! TTTTCCAAAGATAGAATCAT+GGG + chr3_4:78735122-78735141 None:intergenic 25.0%
!! ATTTTCCAAAGATAGAATCA+TGG + chr3_4:78735123-78735142 None:intergenic 25.0%
!! TTCTTTATCTACTCATTTTC+TGG - chr3_4:78735363-78735382 Msa0525200:intron 25.0%
! AATGATATTACCATCAGTTA+CGG + chr3_4:78735405-78735424 None:intergenic 25.0%
! TTGACTTTCTTGTTGATATA+TGG - chr3_4:78735440-78735459 Msa0525200:intron 25.0%
! TGACTTTCTTGTTGATATAT+GGG - chr3_4:78735441-78735460 Msa0525200:intron 25.0%
! TTCATCCTCTTAATGTTTAT+TGG - chr3_4:78735545-78735564 Msa0525200:intron 25.0%
! ACTATCCAATAAACATTAAG+AGG + chr3_4:78735553-78735572 None:intergenic 25.0%
!!! TTTTTATTTTTTCTGTGTGG+AGG - chr3_4:78735600-78735619 Msa0525200:CDS 25.0%
!! TTCCTGATTTTCTATTAAGT+TGG - chr3_4:78735665-78735684 Msa0525200:intron 25.0%
! AACCAACTTAATAGAAAATC+AGG + chr3_4:78735670-78735689 None:intergenic 25.0%
! AAAAGACTTAGTACTTACTT+TGG + chr3_4:78735712-78735731 None:intergenic 25.0%
!! AAGTCTTTTTGTGTGTAAAT+CGG - chr3_4:78735723-78735742 Msa0525200:intron 25.0%
! TTATTAATAACTGACCCAAT+AGG - chr3_4:78735819-78735838 Msa0525200:intron 25.0%
! GGAATCGCTTTAAATATATG+TGG - chr3_4:78734006-78734025 Msa0525200:three_prime_UTR 30.0%
TTACAAGCAATTGATAAGGA+TGG + chr3_4:78734155-78734174 None:intergenic 30.0%
!! AACAATGGGTTGGAAATAAA+AGG - chr3_4:78734227-78734246 Msa0525200:three_prime_UTR 30.0%
TAGAAAGGATGGTAGAAAAT+AGG - chr3_4:78734551-78734570 Msa0525200:CDS 30.0%
AGTAAGACCTAGAAACTATT+AGG - chr3_4:78734666-78734685 Msa0525200:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - chr3_4:78734691-78734710 Msa0525200:intron 30.0%
GATTTAGAGGTTAATGAGTT+GGG - chr3_4:78734692-78734711 Msa0525200:intron 30.0%
GTTATATCCGAAACCATATT+TGG + chr3_4:78734719-78734738 None:intergenic 30.0%
TGGTTTCGGATATAACATTA+TGG - chr3_4:78734723-78734742 Msa0525200:intron 30.0%
TAGAGAAATTAATTGAGAGG+TGG - chr3_4:78735001-78735020 Msa0525200:intron 30.0%
GAAATTAGGAGACGTTAATT+AGG + chr3_4:78735100-78735119 None:intergenic 30.0%
AGATAGAATCATGGGAAATT+AGG + chr3_4:78735114-78735133 None:intergenic 30.0%
ATTTCCCATGATTCTATCTT+TGG - chr3_4:78735115-78735134 Msa0525200:intron 30.0%
ATGAAAGAACTTACTCTAAG+AGG + chr3_4:78735333-78735352 None:intergenic 30.0%
TGTTGATATATGGGAATTAG+AGG - chr3_4:78735450-78735469 Msa0525200:intron 30.0%
GTTGATATATGGGAATTAGA+GGG - chr3_4:78735451-78735470 Msa0525200:intron 30.0%
!!! TTTTTTAAATAGCAGCGCTT+AGG + chr3_4:78735794-78735813 None:intergenic 30.0%
GTGAAAACATAACACCTATT+GGG + chr3_4:78735836-78735855 None:intergenic 30.0%
AGTGAAAACATAACACCTAT+TGG + chr3_4:78735837-78735856 None:intergenic 30.0%
!! AATAGGTGTTATGTTTTCAC+TGG - chr3_4:78735836-78735855 Msa0525200:intron 30.0%
GACACTTGTATTTATGATCT+TGG - chr3_4:78735862-78735881 Msa0525200:intron 30.0%
!!! GTGTTTTTTTTCCACAACTA+AGG - chr3_4:78736023-78736042 Msa0525200:CDS 30.0%
!!! TTCTTTGTTTTCTACCTTAG+TGG - chr3_4:78736187-78736206 Msa0525200:CDS 30.0%
TCGCCCATTAATAAAACAAG+AGG + chr3_4:78733685-78733704 None:intergenic 35.0%
!!! TCTTGTTTTATTAATGGGCG+AGG - chr3_4:78733684-78733703 Msa0525200:three_prime_UTR 35.0%
! TTTTAGTCCCTTAGATGCAT+CGG + chr3_4:78733710-78733729 None:intergenic 35.0%
GTTGTCGTTGTTCATTGTTT+GGG + chr3_4:78733740-78733759 None:intergenic 35.0%
TGTTGTCGTTGTTCATTGTT+TGG + chr3_4:78733741-78733760 None:intergenic 35.0%
! AGTGCTATTGAATTGGATCA+TGG - chr3_4:78733787-78733806 Msa0525200:three_prime_UTR 35.0%
TCTCCAGAATTTGTCAATCA+TGG - chr3_4:78733898-78733917 Msa0525200:three_prime_UTR 35.0%
TTGCCATGATTGACAAATTC+TGG + chr3_4:78733904-78733923 None:intergenic 35.0%
AGGGGTGATAATAGATAAAC+TGG + chr3_4:78733942-78733961 None:intergenic 35.0%
!! TGGTAAGTTTTGATGTATGC+AGG - chr3_4:78734178-78734197 Msa0525200:three_prime_UTR 35.0%
TATAAGGTTTCTGCATGAAG+AGG - chr3_4:78734297-78734316 Msa0525200:three_prime_UTR 35.0%
CAAGGGCAAAAATGATCAAA+TGG + chr3_4:78734373-78734392 None:intergenic 35.0%
TTGGTAAGACTAGACTAGAT+AGG - chr3_4:78734482-78734501 Msa0525200:intron 35.0%
AGACTAGACTAGATAGGATT+AGG - chr3_4:78734488-78734507 Msa0525200:intron 35.0%
ACACAATTAGAGAGAGAGTT+GGG - chr3_4:78734515-78734534 Msa0525200:intron 35.0%
TCTACCATCCTTTCTACTAT+AGG + chr3_4:78734547-78734566 None:intergenic 35.0%
AAAATAGGCTTAGGTGGTTT+GGG - chr3_4:78734566-78734585 Msa0525200:CDS 35.0%
ACAAAGAGTAGATAAGATGG+AGG - chr3_4:78734621-78734640 Msa0525200:intron 35.0%
! TAATGAGTTGGGTCCAAATA+TGG - chr3_4:78734703-78734722 Msa0525200:intron 35.0%
TAGTCAAAGAGAATAAGCAC+GGG + chr3_4:78734846-78734865 None:intergenic 35.0%
CTAGTCAAAGAGAATAAGCA+CGG + chr3_4:78734847-78734866 None:intergenic 35.0%
AGAAATTAATTGAGAGGTGG+AGG - chr3_4:78735004-78735023 Msa0525200:intron 35.0%
GAAATTAATTGAGAGGTGGA+GGG - chr3_4:78735005-78735024 Msa0525200:intron 35.0%
TAGGAGACGTTAATTAGGAT+AGG + chr3_4:78735095-78735114 None:intergenic 35.0%
TGATTATAATTGGGACCAGA+AGG - chr3_4:78735196-78735215 Msa0525200:intron 35.0%
! ATCTTAAAAAGTACGCCTTC+TGG + chr3_4:78735214-78735233 None:intergenic 35.0%
TTCGGATTTATGTTCCTAGT+TGG - chr3_4:78735253-78735272 Msa0525200:intron 35.0%
ATTACCATCAGTTACGGTTA+GGG + chr3_4:78735399-78735418 None:intergenic 35.0%
TATTACCATCAGTTACGGTT+AGG + chr3_4:78735400-78735419 None:intergenic 35.0%
ATGGTAATATCATTCGTGAC+AGG - chr3_4:78735411-78735430 Msa0525200:intron 35.0%
TATGATTGAACTGGCAAAAC+AGG - chr3_4:78735478-78735497 Msa0525200:intron 35.0%
CTGGAAGGAAATGTTAATCT+TGG + chr3_4:78735898-78735917 None:intergenic 35.0%
ACAGTATGAGATGATTCCTA+CGG + chr3_4:78735926-78735945 None:intergenic 35.0%
ATCATCTCATACTGTGACAT+TGG - chr3_4:78735931-78735950 Msa0525200:intron 35.0%
! CACAAAGTTGTTCTGTTTTC+AGG - chr3_4:78735976-78735995 Msa0525200:CDS 35.0%
AATGGATGACATGCTTCTCT+GGG + chr3_4:78733824-78733843 None:intergenic 40.0%
AAATGGATGACATGCTTCTC+TGG + chr3_4:78733825-78733844 None:intergenic 40.0%
ATCAGAAGGATGGTTTGACA+CGG + chr3_4:78733848-78733867 None:intergenic 40.0%
! TACTTTGAGGATCAGAAGGA+TGG + chr3_4:78733858-78733877 None:intergenic 40.0%
! TCACTACTTTGAGGATCAGA+AGG + chr3_4:78733862-78733881 None:intergenic 40.0%
TCTGATCCTCAAAGTAGTGA+TGG - chr3_4:78733862-78733881 Msa0525200:three_prime_UTR 40.0%
TGAATGCCATCACTACTTTG+AGG + chr3_4:78733871-78733890 None:intergenic 40.0%
TTCTTCTGTGGAACCAAACA+AGG - chr3_4:78734203-78734222 Msa0525200:three_prime_UTR 40.0%
GAACCAAACAAGGCAACAAT+GGG - chr3_4:78734213-78734232 Msa0525200:three_prime_UTR 40.0%
! TGGGTTGGAAATAAAAGGTC+CGG - chr3_4:78734232-78734251 Msa0525200:three_prime_UTR 40.0%
AAGTTGGGGAACCCAAAATA+AGG - chr3_4:78734266-78734285 Msa0525200:three_prime_UTR 40.0%
! TTTTGCCCTTGCATGTTGTA+TGG - chr3_4:78734382-78734401 Msa0525200:CDS 40.0%
TGGGCGGTTAAAAATCAACA+CGG - chr3_4:78734415-78734434 Msa0525200:intron 40.0%
CGGAAATCAAGTAAGTGTAG+CGG - chr3_4:78734435-78734454 Msa0525200:intron 40.0%
GACACAATTAGAGAGAGAGT+TGG - chr3_4:78734514-78734533 Msa0525200:intron 40.0%
CACAATTAGAGAGAGAGTTG+GGG - chr3_4:78734516-78734535 Msa0525200:intron 40.0%
AGCACCTATAGTAGAAAGGA+TGG - chr3_4:78734540-78734559 Msa0525200:CDS 40.0%
GGATGGTAGAAAATAGGCTT+AGG - chr3_4:78734557-78734576 Msa0525200:CDS 40.0%
TGGTAGAAAATAGGCTTAGG+TGG - chr3_4:78734560-78734579 Msa0525200:CDS 40.0%
GAAAATAGGCTTAGGTGGTT+TGG - chr3_4:78734565-78734584 Msa0525200:CDS 40.0%
GTTGGGTCCAAATATGGTTT+CGG - chr3_4:78734709-78734728 Msa0525200:intron 40.0%
CGTGCTTATTCTCTTTGACT+AGG - chr3_4:78734845-78734864 Msa0525200:intron 40.0%
GTTAATTAGGATAGGAGGCA+GGG + chr3_4:78735087-78735106 None:intergenic 40.0%
GAGACGTTAATTAGGATAGG+AGG + chr3_4:78735092-78735111 None:intergenic 40.0%
! TATTCCCCTCTAGATTTTGC+AGG - chr3_4:78735505-78735524 Msa0525200:intron 40.0%
! TAGATTTTGCAGGAGAGGAA+AGG - chr3_4:78735515-78735534 Msa0525200:intron 40.0%
! AGTACTTACTTTGGTGCAGA+TGG + chr3_4:78735703-78735722 None:intergenic 40.0%
TGTAAATCGGATATCCTCTG+CGG - chr3_4:78735736-78735755 Msa0525200:intron 40.0%
!! GGTGTTATGTTTTCACTGGA+TGG - chr3_4:78735840-78735859 Msa0525200:intron 40.0%
GATCAAATAGTCTGCAATCC+AGG - chr3_4:78736149-78736168 Msa0525200:CDS 40.0%
GAATTGATCCTCTATAGTCC+TGG + chr3_4:78736170-78736189 None:intergenic 40.0%
! GAGCAGCAGTGCTATTGAAT+TGG - chr3_4:78733780-78733799 Msa0525200:three_prime_UTR 45.0%
AGGATGGTTTGACACGGAAA+TGG + chr3_4:78733842-78733861 None:intergenic 45.0%
! ATGTGGTTGCTTTCCGTTCA+TGG - chr3_4:78734023-78734042 Msa0525200:three_prime_UTR 45.0%
TGTATGCAGGTCTTCTTCTG+TGG - chr3_4:78734191-78734210 Msa0525200:three_prime_UTR 45.0%
GGAACCAAACAAGGCAACAA+TGG - chr3_4:78734212-78734231 Msa0525200:three_prime_UTR 45.0%
! CAACCCATTGTTGCCTTGTT+TGG + chr3_4:78734219-78734238 None:intergenic 45.0%
CAAACAAGGCAACAATGGGT+TGG - chr3_4:78734217-78734236 Msa0525200:three_prime_UTR 45.0%
GGGTTGGAAATAAAAGGTCC+GGG - chr3_4:78734233-78734252 Msa0525200:three_prime_UTR 45.0%
! TTGGGTTCCCCAACTTGTTT+CGG + chr3_4:78734262-78734281 None:intergenic 45.0%
GTTTCTGCATGAAGAGGCTT+AGG - chr3_4:78734303-78734322 Msa0525200:three_prime_UTR 45.0%
ATGAAGAGGCTTAGGTGGTT+TGG - chr3_4:78734311-78734330 Msa0525200:three_prime_UTR 45.0%
!! TAGGTGGTTTGGTCATTACC+AGG - chr3_4:78734322-78734341 Msa0525200:three_prime_UTR 45.0%
!! AGGTGGTTTGGTCATTACCA+GGG - chr3_4:78734323-78734342 Msa0525200:three_prime_UTR 45.0%
ATGCAATACTGTACTTGCCC+TGG + chr3_4:78734343-78734362 None:intergenic 45.0%
CCGTACCATACAACATGCAA+GGG + chr3_4:78734390-78734409 None:intergenic 45.0%
CCCTTGCATGTTGTATGGTA+CGG - chr3_4:78734387-78734406 Msa0525200:CDS 45.0%
TCCGTACCATACAACATGCA+AGG + chr3_4:78734391-78734410 None:intergenic 45.0%
TGTTGTATGGTACGGAGTGT+TGG - chr3_4:78734395-78734414 Msa0525200:CDS 45.0%
! GTTGTATGGTACGGAGTGTT+GGG - chr3_4:78734396-78734415 Msa0525200:CDS 45.0%
! AAGTAAGTGTAGCGGAGATG+AGG - chr3_4:78734443-78734462 Msa0525200:intron 45.0%
AGGATGTTGCGTTGGATGAT+TGG - chr3_4:78734463-78734482 Msa0525200:intron 45.0%
GGGTAGCACCTATAGTAGAA+AGG - chr3_4:78734536-78734555 Msa0525200:CDS 45.0%
GAGGAGAGTCTGATATGAAG+AGG - chr3_4:78734640-78734659 Msa0525200:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + chr3_4:78734772-78734791 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + chr3_4:78734773-78734792 None:intergenic 45.0%
CGTTAATTAGGATAGGAGGC+AGG + chr3_4:78735088-78735107 None:intergenic 45.0%
! GAGGGCATGTATGATTGAAC+TGG - chr3_4:78735469-78735488 Msa0525200:intron 45.0%
CTCTCCTGCAAAATCTAGAG+GGG + chr3_4:78735512-78735531 None:intergenic 45.0%
CCTCTCCTGCAAAATCTAGA+GGG + chr3_4:78735513-78735532 None:intergenic 45.0%
TCCTCTCCTGCAAAATCTAG+AGG + chr3_4:78735514-78735533 None:intergenic 45.0%
GTGAAGCTTGACTAAGCTGA+GGG + chr3_4:78735770-78735789 None:intergenic 45.0%
AGTGAAGCTTGACTAAGCTG+AGG + chr3_4:78735771-78735790 None:intergenic 45.0%
CTCATACTGTGACATTGGTG+AGG - chr3_4:78735936-78735955 Msa0525200:intron 45.0%
! CATTGGTGAGGCTTTGATGA+AGG - chr3_4:78735948-78735967 Msa0525200:CDS 45.0%
!!! GTTGTTCTGTTTTCAGGTGC+AGG - chr3_4:78735982-78736001 Msa0525200:CDS 45.0%
TTCCACAACTAAGGCAGTTG+CGG - chr3_4:78736032-78736051 Msa0525200:CDS 45.0%
TCTGCAATCCAGGACTATAG+AGG - chr3_4:78736159-78736178 Msa0525200:CDS 45.0%
CAACTTGGAGATCACCACTA+AGG + chr3_4:78736204-78736223 None:intergenic 45.0%
CTTAGTGGTGATCTCCAAGT+TGG - chr3_4:78736202-78736221 Msa0525200:CDS 45.0%
! TGGTGATCTCCAAGTTGGAA+TGG - chr3_4:78736207-78736226 Msa0525200:CDS 45.0%
! GGTGATCTCCAAGTTGGAAT+GGG - chr3_4:78736208-78736227 Msa0525200:CDS 45.0%
! GTGATCTCCAAGTTGGAATG+GGG - chr3_4:78736209-78736228 Msa0525200:CDS 45.0%
CAACAGAGCAGAGGTTGTCT+TGG - chr3_4:78733646-78733665 Msa0525200:three_prime_UTR 50.0%
TCTGCATGAAGAGGCTTAGG+TGG - chr3_4:78734306-78734325 Msa0525200:three_prime_UTR 50.0%
CTTATCCCACTAAGTGGGGT+CGG + chr3_4:78734768-78734787 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + chr3_4:78734774-78734793 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - chr3_4:78734771-78734790 Msa0525200:intron 50.0%
GAGAATAAGCACGGGCAGTA+AGG + chr3_4:78734838-78734857 None:intergenic 50.0%
GAGGTGGAGGGAGTTAAACT+TGG - chr3_4:78735017-78735036 Msa0525200:intron 50.0%
CCACCTACGAGAGTCTACTT+GGG - chr3_4:78735280-78735299 Msa0525200:intron 50.0%
AGTCCCAAGTAGACTCTCGT+AGG + chr3_4:78735286-78735305 None:intergenic 50.0%
AGAGGAATGCGCTCAGGAAA+AGG + chr3_4:78735315-78735334 None:intergenic 50.0%
! ACTCTAAGAGGAATGCGCTC+AGG + chr3_4:78735321-78735340 None:intergenic 50.0%
TGCTCCCTAACCGTAACTGA+TGG - chr3_4:78735392-78735411 Msa0525200:intron 50.0%
! CCCTCTAGATTTTGCAGGAG+AGG - chr3_4:78735510-78735529 Msa0525200:intron 50.0%
TTTGCAGGAGAGGAAAGGTG+TGG - chr3_4:78735520-78735539 Msa0525200:intron 50.0%
ATCCGCAACTGCCTTAGTTG+TGG + chr3_4:78736037-78736056 None:intergenic 50.0%
! ATCTCCAAGTTGGAATGGGG+CGG - chr3_4:78736212-78736231 Msa0525200:CDS 50.0%
CCTCTGCTCTGTTGCAAAGC+AGG + chr3_4:78733640-78733659 None:intergenic 55.0%
CCTGCTTTGCAACAGAGCAG+AGG - chr3_4:78733637-78733656 Msa0525200:three_prime_UTR 55.0%
!! GTATGGTACGGAGTGTTGGG+CGG - chr3_4:78734399-78734418 Msa0525200:CDS 55.0%
CGGAGATGAGGATGTTGCGT+TGG - chr3_4:78734455-78734474 Msa0525200:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr3_4:78734760-78734779 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr3_4:78734759-78734778 Msa0525200:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr3_4:78734760-78734779 Msa0525200:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG - chr3_4:78734766-78734785 Msa0525200:intron 55.0%
TCGTAGGTGGCGTTCCAACT+AGG + chr3_4:78735270-78735289 None:intergenic 55.0%
GCCACCTACGAGAGTCTACT+TGG - chr3_4:78735279-78735298 Msa0525200:intron 55.0%
CCCAAGTAGACTCTCGTAGG+TGG + chr3_4:78735283-78735302 None:intergenic 55.0%
!! TGAGGGATTAGTCTCCGCAG+AGG + chr3_4:78735753-78735772 None:intergenic 55.0%
TTTCCTTCCAGTGCTGCCGT+AGG - chr3_4:78735907-78735926 Msa0525200:intron 55.0%
! ATTCCTACGGCAGCACTGGA+AGG + chr3_4:78735913-78735932 None:intergenic 55.0%
GATGATTCCTACGGCAGCAC+TGG + chr3_4:78735917-78735936 None:intergenic 55.0%
GGCGAGGCCGATGCATCTAA+GGG - chr3_4:78733700-78733719 Msa0525200:three_prime_UTR 60.0%
TCCGGGCGTCCGAAACAAGT+TGG - chr3_4:78734250-78734269 Msa0525200:three_prime_UTR 60.0%
! CCCAACTTGTTTCGGACGCC+CGG + chr3_4:78734254-78734273 None:intergenic 60.0%
CCGGGCGTCCGAAACAAGTT+GGG - chr3_4:78734251-78734270 Msa0525200:three_prime_UTR 60.0%
CGGGCGTCCGAAACAAGTTG+GGG - chr3_4:78734252-78734271 Msa0525200:three_prime_UTR 60.0%
GGGCGAGGCCGATGCATCTA+AGG - chr3_4:78733699-78733718 Msa0525200:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 78733622 78736235 78733622 ID=Msa0525200;Name=Msa0525200
chr3_4 mRNA 78733622 78736235 78733622 ID=Msa0525200-mRNA-1;Parent=Msa0525200;Name=Msa0525200-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|1|0.75|1|1|1|4|749|168
chr3_4 exon 78733622 78734424 78733622 ID=Msa0525200-mRNA-1:exon:23445;Parent=Msa0525200-mRNA-1
chr3_4 exon 78734519 78734585 78734519 ID=Msa0525200-mRNA-1:exon:23444;Parent=Msa0525200-mRNA-1
chr3_4 exon 78735570 78735657 78735570 ID=Msa0525200-mRNA-1:exon:23443;Parent=Msa0525200-mRNA-1
chr3_4 exon 78735938 78736235 78735938 ID=Msa0525200-mRNA-1:exon:23442;Parent=Msa0525200-mRNA-1
chr3_4 CDS 78735938 78736235 78735938 ID=Msa0525200-mRNA-1:cds;Parent=Msa0525200-mRNA-1
chr3_4 CDS 78735570 78735657 78735570 ID=Msa0525200-mRNA-1:cds;Parent=Msa0525200-mRNA-1
chr3_4 CDS 78734519 78734585 78734519 ID=Msa0525200-mRNA-1:cds;Parent=Msa0525200-mRNA-1
chr3_4 CDS 78734371 78734424 78734371 ID=Msa0525200-mRNA-1:cds;Parent=Msa0525200-mRNA-1
chr3_4 three_prime_UTR 78733622 78734370 78733622 ID=Msa0525200-mRNA-1:three_prime_utr;Parent=Msa0525200-mRNA-1
Gene Sequence

>Msa0525200

ATGCTTCTGGTTAAACCTGCTTTGCAACAGAGCAGAGGTTGTCTTGGATGTTTCTCAAAACCTCTTGTTTTATTAATGGGCGAGGCCGATGCATCTAAGGGACTAAAAACTCAAGCCCAAACAATGAACAACGACAACAGATCTGAAGATTTTTTTTGGAGCAGCAGTGCTATTGAATTGGATCATGGTGCTATTCAGTCCCAGAGAAGCATGTCATCCATTTCCGTGTCAAACCATCCTTCTGATCCTCAAAGTAGTGATGGCATTCAGACTGATTCTCCAGAATTTGTCAATCATGGTCTTCTTCTGTGGAACCAAACAAGGCAACAATGGGTTGGAAATAAAAGGTCCGGGCGTCCGAAACAAGTTGGGGAACCCAAAATAAGTTGGAACGCCACCTACGAGAGTCTACTTGGGACTAACAAGCCTTTTCCTGAGCGCATTCCTCTTAGAGAAATGATTGACTTTCTTGTTGATATATGGGAATTAGAGGGCATGTATGATTGA
Protein sequence

>Msa0525200

MLLVKPALQQSRGCLGCFSKPLVLLMGEADASKGLKTQAQTMNNDNRSEDFFWSSSAIELDHGAIQSQRSMSSISVSNHPSDPQSSDGIQTDSPEFVNHGLLLWNQTRQQWVGNKRSGRPKQVGEPKISWNATYESLLGTNKPFPERIPLREMIDFLVDIWELEGMYD*