AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1435620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435620 MtrunA17_Chr3g0137781 96.178 157 6 0 12 168 21 177 2.15e-112 317
Msa1435620 MtrunA17_Chr4g0003351 50.943 159 72 4 11 168 6 159 7.67e-52 162
Msa1435620 MtrunA17_Chr4g0063831 50.943 159 69 5 13 167 18 171 5.74e-45 145
Msa1435620 MtrunA17_Chr1g0149341 55.714 140 60 2 30 168 3 141 1.55e-34 118
Msa1435620 MtrunA17_Chr1g0199151 51.515 66 31 1 104 168 21 86 1.19e-19 78.6
Msa1435620 MtrunA17_Chr7g0264721 37.500 80 46 2 91 168 28 105 1.73e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435620 AT5G25360.3 55.128 156 64 3 13 168 67 216 8.24e-53 167
Msa1435620 AT5G25360.2 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa1435620 AT5G25360.1 55.405 148 59 3 21 168 29 169 1.33e-48 155
Msa1435620 AT4G32342.1 49.091 165 66 5 7 167 10 160 5.10e-45 145
Msa1435620 AT4G32342.2 49.091 165 66 5 7 167 48 198 1.59e-44 145
Msa1435620 AT1G15350.1 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa1435620 AT1G15350.2 45.570 158 78 5 13 168 3 154 5.59e-37 125
Msa1435620 AT3G15770.2 53.719 121 51 3 48 167 44 160 2.52e-36 124
Msa1435620 AT3G15770.1 53.719 121 51 3 48 167 45 161 2.77e-36 124
Msa1435620 AT1G15350.6 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa1435620 AT1G15350.3 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa1435620 AT1G15350.5 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa1435620 AT1G15350.4 55.446 101 43 2 70 168 8 108 3.78e-31 108
Msa1435620 AT3G54880.1 41.228 114 62 2 58 168 1 112 9.60e-24 90.1
Msa1435620 AT5G03440.2 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa1435620 AT5G03440.3 42.857 84 48 0 85 168 15 98 7.61e-20 79.7
Msa1435620 AT5G03440.1 42.857 84 48 0 85 168 15 98 7.61e-20 79.7

Find 89 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTTATTTACCTTATTT+TGG 0.062991 tig0027765:+8322 None:intergenic
TTCATCCTCTTAATGTTTAT+TGG 0.159120 tig0027765:-7055 Msa1435620:three_prime_UTR
TTGCCATGATTGACAAATTC+TGG 0.203050 tig0027765:+8697 None:intergenic
TTGACTTTCTTGTTGATATA+TGG 0.216717 tig0027765:-7160 Msa1435620:CDS
CAACCCATTGTTGCCTTGTT+TGG 0.223693 tig0027765:+8384 None:intergenic
GACACTTGTATTTATGATCT+TGG 0.275845 tig0027765:-6738 Msa1435620:three_prime_UTR
GGGCGAGGCCGATGCATCTA+AGG 0.287521 tig0027765:-8899 Msa1435620:CDS
AAATGGATGACATGCTTCTC+TGG 0.301324 tig0027765:+8776 None:intergenic
TGTTGTCGTTGTTCATTGTT+TGG 0.311681 tig0027765:+8860 None:intergenic
AACAATGGGTTGGAAATAAA+AGG 0.312465 tig0027765:-8373 Msa1435620:CDS
AGTGAAAACATAACACCTAT+TGG 0.318943 tig0027765:+6766 None:intergenic
GGTTGTGTTAATCATGCTTC+TGG 0.347483 tig0027765:-8989 None:intergenic
GAGCAGCAGTGCTATTGAAT+TGG 0.355690 tig0027765:-8818 Msa1435620:CDS
TTGGGTTCCCCAACTTGTTT+CGG 0.356307 tig0027765:+8341 None:intergenic
TATGATTGAACTGGCAAAAC+AGG 0.376802 tig0027765:-7122 Msa1435620:exon
AACCAACTTAATAGAAAATC+AGG 0.378292 tig0027765:+6933 None:intergenic
TGACTTTCTTGTTGATATAT+GGG 0.383956 tig0027765:-7159 Msa1435620:CDS
AAGTTGGGGAACCCAAAATA+AGG 0.384880 tig0027765:-8334 Msa1435620:intron
TGTTGATATATGGGAATTAG+AGG 0.391346 tig0027765:-7150 Msa1435620:CDS
ACTATCCAATAAACATTAAG+AGG 0.394734 tig0027765:+7050 None:intergenic
CCCAACTTGTTTCGGACGCC+CGG 0.401219 tig0027765:+8349 None:intergenic
CCTCTGCTCTGTTGCAAAGC+AGG 0.407496 tig0027765:+8961 None:intergenic
TCTCCAGAATTTGTCAATCA+TGG 0.416899 tig0027765:-8700 Msa1435620:intron
CCGGGCGTCCGAAACAAGTT+GGG 0.420971 tig0027765:-8349 Msa1435620:CDS
CAACAGAGCAGAGGTTGTCT+TGG 0.421310 tig0027765:-8952 Msa1435620:CDS
CTGGAAGGAAATGTTAATCT+TGG 0.422072 tig0027765:+6705 None:intergenic
GCCACCTACGAGAGTCTACT+TGG 0.426114 tig0027765:-7321 Msa1435620:CDS
AAAAGACTTAGTACTTACTT+TGG 0.426312 tig0027765:+6891 None:intergenic
GTGAAAACATAACACCTATT+GGG 0.432741 tig0027765:+6767 None:intergenic
GTTGTCGTTGTTCATTGTTT+GGG 0.444475 tig0027765:+8861 None:intergenic
TGGGTTGGAAATAAAAGGTC+CGG 0.452762 tig0027765:-8368 Msa1435620:CDS
GGTGATCTCCAAGTTGGAAT+GGG 0.452928 tig0027765:-6392 Msa1435620:three_prime_UTR
ATGAAAGAACTTACTCTAAG+AGG 0.460791 tig0027765:+7270 None:intergenic
AGGATGGTTTGACACGGAAA+TGG 0.461819 tig0027765:+8759 None:intergenic
AGAGGAATGCGCTCAGGAAA+AGG 0.472233 tig0027765:+7288 None:intergenic
GATCAAATAGTCTGCAATCC+AGG 0.473644 tig0027765:-6451 Msa1435620:three_prime_UTR
TGAATGCCATCACTACTTTG+AGG 0.479975 tig0027765:+8730 None:intergenic
GAGGGCATGTATGATTGAAC+TGG 0.485460 tig0027765:-7131 Msa1435620:exon
GATGATTCCTACGGCAGCAC+TGG 0.489025 tig0027765:+6686 None:intergenic
TCCGGGCGTCCGAAACAAGT+TGG 0.490712 tig0027765:-8350 Msa1435620:CDS
TTATTAATAACTGACCCAAT+AGG 0.491022 tig0027765:-6781 Msa1435620:three_prime_UTR
ATCATCTCATACTGTGACAT+TGG 0.493232 tig0027765:-6669 Msa1435620:three_prime_UTR
TGGTGATCTCCAAGTTGGAA+TGG 0.502647 tig0027765:-6393 Msa1435620:three_prime_UTR
GAACCAAACAAGGCAACAAT+GGG 0.503294 tig0027765:-8387 Msa1435620:CDS
CTTAGTGGTGATCTCCAAGT+TGG 0.503397 tig0027765:-6398 Msa1435620:three_prime_UTR
TTCTTCTGTGGAACCAAACA+AGG 0.508337 tig0027765:-8397 Msa1435620:CDS
AGTACTTACTTTGGTGCAGA+TGG 0.516361 tig0027765:+6900 None:intergenic
TCTGCAATCCAGGACTATAG+AGG 0.524169 tig0027765:-6441 Msa1435620:three_prime_UTR
CATTGGTGAGGCTTTGATGA+AGG 0.524390 tig0027765:-6652 Msa1435620:three_prime_UTR
ACTCTAAGAGGAATGCGCTC+AGG 0.532544 tig0027765:+7282 None:intergenic
TTCCACAACTAAGGCAGTTG+CGG 0.533016 tig0027765:-6568 Msa1435620:three_prime_UTR
GAATTGATCCTCTATAGTCC+TGG 0.533065 tig0027765:+6433 None:intergenic
TACTTTGAGGATCAGAAGGA+TGG 0.543246 tig0027765:+8743 None:intergenic
GCTCCCGCCCCATTCCAACT+TGG 0.549677 tig0027765:+6384 None:intergenic
GGAACCAAACAAGGCAACAA+TGG 0.556597 tig0027765:-8388 Msa1435620:CDS
AATGGATGACATGCTTCTCT+GGG 0.557186 tig0027765:+8777 None:intergenic
ATCCGCAACTGCCTTAGTTG+TGG 0.558439 tig0027765:+6566 None:intergenic
GGCGAGGCCGATGCATCTAA+GGG 0.563203 tig0027765:-8898 Msa1435620:CDS
TCTCCAAGTTGGAATGGGGC+GGG 0.563451 tig0027765:-6387 Msa1435620:three_prime_UTR
TGTATGCAGGTCTTCTTCTG+TGG 0.571627 tig0027765:-8409 Msa1435620:intron
TCCTCTCCTGCAAAATCTAG+AGG 0.574443 tig0027765:+7089 None:intergenic
CCTCTCCTGCAAAATCTAGA+GGG 0.578403 tig0027765:+7090 None:intergenic
TCTGATCCTCAAAGTAGTGA+TGG 0.580218 tig0027765:-8736 Msa1435620:CDS
ATTCCTACGGCAGCACTGGA+AGG 0.586917 tig0027765:+6690 None:intergenic
CAAACAAGGCAACAATGGGT+TGG 0.588867 tig0027765:-8383 Msa1435620:CDS
GGGTTGGAAATAAAAGGTCC+GGG 0.591087 tig0027765:-8367 Msa1435620:CDS
ATCTCCAAGTTGGAATGGGG+CGG 0.592965 tig0027765:-6388 Msa1435620:three_prime_UTR
GTTGATATATGGGAATTAGA+GGG 0.593196 tig0027765:-7149 Msa1435620:CDS
TCGCCCATTAATAAAACAAG+AGG 0.595108 tig0027765:+8916 None:intergenic
TCACTACTTTGAGGATCAGA+AGG 0.595919 tig0027765:+8739 None:intergenic
CAACTTGGAGATCACCACTA+AGG 0.598538 tig0027765:+6399 None:intergenic
TTTGCAGGAGAGGAAAGGTG+TGG 0.612135 tig0027765:-7080 Msa1435620:three_prime_UTR
ACTATTTGATCAAAATAAGA+CGG 0.613083 tig0027765:+6463 None:intergenic
TTTCCTTCCAGTGCTGCCGT+AGG 0.617308 tig0027765:-6693 Msa1435620:three_prime_UTR
AGTGCTATTGAATTGGATCA+TGG 0.626780 tig0027765:-8811 Msa1435620:CDS
ACAGTATGAGATGATTCCTA+CGG 0.628284 tig0027765:+6677 None:intergenic
CTCATACTGTGACATTGGTG+AGG 0.628731 tig0027765:-6664 Msa1435620:three_prime_UTR
CCTGCTTTGCAACAGAGCAG+AGG 0.631103 tig0027765:-8961 Msa1435620:CDS
AGTGAAGCTTGACTAAGCTG+AGG 0.634103 tig0027765:+6832 None:intergenic
GTGATCTCCAAGTTGGAATG+GGG 0.637300 tig0027765:-6391 Msa1435620:three_prime_UTR
GTGAAGCTTGACTAAGCTGA+GGG 0.640473 tig0027765:+6833 None:intergenic
CTCTCCTGCAAAATCTAGAG+GGG 0.644947 tig0027765:+7091 None:intergenic
CCCAAGTAGACTCTCGTAGG+TGG 0.647046 tig0027765:+7320 None:intergenic
ATCAGAAGGATGGTTTGACA+CGG 0.658576 tig0027765:+8753 None:intergenic
CGGGCGTCCGAAACAAGTTG+GGG 0.664193 tig0027765:-8348 Msa1435620:CDS
AGTCCCAAGTAGACTCTCGT+AGG 0.676556 tig0027765:+7317 None:intergenic
CCACCTACGAGAGTCTACTT+GGG 0.677939 tig0027765:-7320 Msa1435620:CDS
TGAGGGATTAGTCTCCGCAG+AGG 0.727780 tig0027765:+6850 None:intergenic
TGTAAATCGGATATCCTCTG+CGG 0.750038 tig0027765:-6864 Msa1435620:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATTTACCTTATTTT+GGG + tig0027765:7043-7062 None:intergenic 10.0%
!!! TATATTTATTTACCTTATTT+TGG + tig0027765:7044-7063 None:intergenic 10.0%
!! AAATAAGGTAAATAAATATA+AGG - tig0027765:7044-7063 Msa1435620:three_prime_UTR 10.0%
!!! TTTTTTTTTCTGATTATAAT+TGG - tig0027765:7949-7968 Msa1435620:intron 10.0%
!!! TTTTTTTTCTGATTATAATT+GGG - tig0027765:7950-7969 Msa1435620:intron 10.0%
!! AAAGAAAATATACAAATAGA+GGG + tig0027765:6726-6745 None:intergenic 15.0%
!! AAAAGAAAATATACAAATAG+AGG + tig0027765:6727-6746 None:intergenic 15.0%
!!! ATTTTTTTCTTTTATCTTGA+TGG - tig0027765:6749-6768 Msa1435620:three_prime_UTR 15.0%
!! CAAAATATAATAAAAAGACA+AGG + tig0027765:6893-6912 None:intergenic 15.0%
!!! AAAACCTCTTGTTTTATTAA+TGG - tig0027765:6443-6462 Msa1435620:three_prime_UTR 20.0%
!!! AAACCTCTTGTTTTATTAAT+GGG - tig0027765:6444-6463 Msa1435620:three_prime_UTR 20.0%
!! AAGAAAATATACAAATAGAG+GGG + tig0027765:6725-6744 None:intergenic 20.0%
!! ATTATTACAAGCAATTGATA+AGG + tig0027765:6922-6941 None:intergenic 20.0%
!! TTATCAATTGCTTGTAATAA+TGG - tig0027765:6921-6940 Msa1435620:three_prime_UTR 20.0%
!! TAAAAAATGTATGAGTGATA+AGG - tig0027765:7718-7737 Msa1435620:intron 20.0%
!!! TTTTGTCAAGTTCTAATTTT+CGG - tig0027765:7998-8017 Msa1435620:intron 20.0%
!!! TTGTTTTTATTTTTTCTGTG+TGG - tig0027765:8360-8379 Msa1435620:CDS 20.0%
!! ACTATTTGATCAAAATAAGA+CGG + tig0027765:8903-8922 None:intergenic 20.0%
!!! ACAGATCTGAAGATTTTTTT+TGG - tig0027765:6523-6542 Msa1435620:three_prime_UTR 25.0%
!! TTAACTTTAAGAACCATGAA+CGG + tig0027765:6803-6822 None:intergenic 25.0%
!!! CTTTCTTCTATCGATTTTAT+AGG - tig0027765:6863-6882 Msa1435620:three_prime_UTR 25.0%
! CTTTGTATTACAACATCTAT+CGG + tig0027765:7370-7389 None:intergenic 25.0%
! AATACAAAGAGTAGATAAGA+TGG - tig0027765:7381-7400 Msa1435620:intron 25.0%
! AATCTTTCCTAATAGTTTCT+AGG + tig0027765:7439-7458 None:intergenic 25.0%
! AACTATTAGGAAAGATTTAG+AGG - tig0027765:7442-7461 Msa1435620:intron 25.0%
! GGAAAAAAATGTACAAACAA+GGG + tig0027765:7580-7599 None:intergenic 25.0%
! AGGAAAAAAATGTACAAACA+AGG + tig0027765:7581-7600 None:intergenic 25.0%
! TGGTCTAAACAATGAATAAA+AGG + tig0027765:7677-7696 None:intergenic 25.0%
!! CTTGAAGCAAAAGATTTTTA+TGG + tig0027765:7697-7716 None:intergenic 25.0%
! AAGTAGAGAAATTAATTGAG+AGG - tig0027765:7761-7780 Msa1435620:intron 25.0%
!! TTTTCCAAAGATAGAATCAT+GGG + tig0027765:7885-7904 None:intergenic 25.0%
!! ATTTTCCAAAGATAGAATCA+TGG + tig0027765:7886-7905 None:intergenic 25.0%
!! TTCTTTATCTACTCATTTTC+TGG - tig0027765:8126-8145 Msa1435620:intron 25.0%
! AATGATATTACCATCAGTTA+CGG + tig0027765:8168-8187 None:intergenic 25.0%
! TTGACTTTCTTGTTGATATA+TGG - tig0027765:8203-8222 Msa1435620:intron 25.0%
! TGACTTTCTTGTTGATATAT+GGG - tig0027765:8204-8223 Msa1435620:intron 25.0%
! TTCATCCTCTTAATGTTTAT+TGG - tig0027765:8308-8327 Msa1435620:intron 25.0%
! ACTATCCAATAAACATTAAG+AGG + tig0027765:8316-8335 None:intergenic 25.0%
!!! TTTTTATTTTTTCTGTGTGG+AGG - tig0027765:8363-8382 Msa1435620:CDS 25.0%
!! TTCCTGATTTTCTATTAAGT+TGG - tig0027765:8428-8447 Msa1435620:intron 25.0%
! AACCAACTTAATAGAAAATC+AGG + tig0027765:8433-8452 None:intergenic 25.0%
! AAAAGACTTAGTACTTACTT+TGG + tig0027765:8475-8494 None:intergenic 25.0%
!! AAGTCTTTTTGTGTGTAAAT+CGG - tig0027765:8486-8505 Msa1435620:intron 25.0%
! TTATTAATAACTGACCCAAT+AGG - tig0027765:8582-8601 Msa1435620:intron 25.0%
! GGAATCGCTTTAAATATATG+TGG - tig0027765:6770-6789 Msa1435620:three_prime_UTR 30.0%
TTACAAGCAATTGATAAGGA+TGG + tig0027765:6918-6937 None:intergenic 30.0%
!! AACAATGGGTTGGAAATAAA+AGG - tig0027765:6990-7009 Msa1435620:three_prime_UTR 30.0%
TAGAAAGGATGGTAGAAAAT+AGG - tig0027765:7314-7333 Msa1435620:CDS 30.0%
AGTAAGACCTAGAAACTATT+AGG - tig0027765:7429-7448 Msa1435620:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - tig0027765:7454-7473 Msa1435620:intron 30.0%
GATTTAGAGGTTAATGAGTT+GGG - tig0027765:7455-7474 Msa1435620:intron 30.0%
GTTATATCCGAAACCATATT+TGG + tig0027765:7482-7501 None:intergenic 30.0%
TGGTTTCGGATATAACATTA+TGG - tig0027765:7486-7505 Msa1435620:intron 30.0%
TAGAGAAATTAATTGAGAGG+TGG - tig0027765:7764-7783 Msa1435620:intron 30.0%
GAAATTAGGAGACGTTAATT+AGG + tig0027765:7863-7882 None:intergenic 30.0%
AGATAGAATCATGGGAAATT+AGG + tig0027765:7877-7896 None:intergenic 30.0%
ATTTCCCATGATTCTATCTT+TGG - tig0027765:7878-7897 Msa1435620:intron 30.0%
ATGAAAGAACTTACTCTAAG+AGG + tig0027765:8096-8115 None:intergenic 30.0%
TGTTGATATATGGGAATTAG+AGG - tig0027765:8213-8232 Msa1435620:intron 30.0%
GTTGATATATGGGAATTAGA+GGG - tig0027765:8214-8233 Msa1435620:intron 30.0%
!!! TTTTTTAAATAGCAGCGCTT+AGG + tig0027765:8557-8576 None:intergenic 30.0%
GTGAAAACATAACACCTATT+GGG + tig0027765:8599-8618 None:intergenic 30.0%
AGTGAAAACATAACACCTAT+TGG + tig0027765:8600-8619 None:intergenic 30.0%
!! AATAGGTGTTATGTTTTCAC+TGG - tig0027765:8599-8618 Msa1435620:intron 30.0%
GACACTTGTATTTATGATCT+TGG - tig0027765:8625-8644 Msa1435620:intron 30.0%
!!! GTGTTTTTTTTCCACAACTA+AGG - tig0027765:8786-8805 Msa1435620:CDS 30.0%
!!! TTCTTTGTTTTCTACCTTAG+TGG - tig0027765:8950-8969 Msa1435620:CDS 30.0%
TCGCCCATTAATAAAACAAG+AGG + tig0027765:6450-6469 None:intergenic 35.0%
!!! TCTTGTTTTATTAATGGGCG+AGG - tig0027765:6449-6468 Msa1435620:three_prime_UTR 35.0%
! TTTTAGTCCCTTAGATGCAT+CGG + tig0027765:6475-6494 None:intergenic 35.0%
GTTGTCGTTGTTCATTGTTT+GGG + tig0027765:6505-6524 None:intergenic 35.0%
TGTTGTCGTTGTTCATTGTT+TGG + tig0027765:6506-6525 None:intergenic 35.0%
! AGTGCTATTGAATTGGATCA+TGG - tig0027765:6552-6571 Msa1435620:three_prime_UTR 35.0%
TCTCCAGAATTTGTCAATCA+TGG - tig0027765:6663-6682 Msa1435620:three_prime_UTR 35.0%
TTGCCATGATTGACAAATTC+TGG + tig0027765:6669-6688 None:intergenic 35.0%
AGGGGTGATAATAGATAAAC+TGG + tig0027765:6707-6726 None:intergenic 35.0%
!! TGGTAAGTTTTGATGTATGC+AGG - tig0027765:6941-6960 Msa1435620:three_prime_UTR 35.0%
TATAAGGTTTCTGCATGAAG+AGG - tig0027765:7060-7079 Msa1435620:three_prime_UTR 35.0%
CAAGGGCAAAAATGATCAAA+TGG + tig0027765:7136-7155 None:intergenic 35.0%
TTGGTAAGACTAGACTAGAT+AGG - tig0027765:7245-7264 Msa1435620:intron 35.0%
AGACTAGACTAGATAGGATT+AGG - tig0027765:7251-7270 Msa1435620:intron 35.0%
ACACAATTAGAGAGAGAGTT+GGG - tig0027765:7278-7297 Msa1435620:intron 35.0%
TCTACCATCCTTTCTACTAT+AGG + tig0027765:7310-7329 None:intergenic 35.0%
AAAATAGGCTTAGGTGGTTT+GGG - tig0027765:7329-7348 Msa1435620:CDS 35.0%
ACAAAGAGTAGATAAGATGG+AGG - tig0027765:7384-7403 Msa1435620:intron 35.0%
! TAATGAGTTGGGTCCAAATA+TGG - tig0027765:7466-7485 Msa1435620:intron 35.0%
TAGTCAAAGAGAATAAGCAC+GGG + tig0027765:7609-7628 None:intergenic 35.0%
CTAGTCAAAGAGAATAAGCA+CGG + tig0027765:7610-7629 None:intergenic 35.0%
AGAAATTAATTGAGAGGTGG+AGG - tig0027765:7767-7786 Msa1435620:intron 35.0%
GAAATTAATTGAGAGGTGGA+GGG - tig0027765:7768-7787 Msa1435620:intron 35.0%
TAGGAGACGTTAATTAGGAT+AGG + tig0027765:7858-7877 None:intergenic 35.0%
TGATTATAATTGGGACCAGA+AGG - tig0027765:7959-7978 Msa1435620:intron 35.0%
! ATCTTAAAAAGTACGCCTTC+TGG + tig0027765:7977-7996 None:intergenic 35.0%
TTCGGATTTATGTTCCTAGT+TGG - tig0027765:8016-8035 Msa1435620:intron 35.0%
ATTACCATCAGTTACGGTTA+GGG + tig0027765:8162-8181 None:intergenic 35.0%
TATTACCATCAGTTACGGTT+AGG + tig0027765:8163-8182 None:intergenic 35.0%
ATGGTAATATCATTCGTGAC+AGG - tig0027765:8174-8193 Msa1435620:intron 35.0%
TATGATTGAACTGGCAAAAC+AGG - tig0027765:8241-8260 Msa1435620:intron 35.0%
CTGGAAGGAAATGTTAATCT+TGG + tig0027765:8661-8680 None:intergenic 35.0%
ACAGTATGAGATGATTCCTA+CGG + tig0027765:8689-8708 None:intergenic 35.0%
ATCATCTCATACTGTGACAT+TGG - tig0027765:8694-8713 Msa1435620:intron 35.0%
! CACAAAGTTGTTCTGTTTTC+AGG - tig0027765:8739-8758 Msa1435620:CDS 35.0%
AATGGATGACATGCTTCTCT+GGG + tig0027765:6589-6608 None:intergenic 40.0%
AAATGGATGACATGCTTCTC+TGG + tig0027765:6590-6609 None:intergenic 40.0%
ATCAGAAGGATGGTTTGACA+CGG + tig0027765:6613-6632 None:intergenic 40.0%
! TACTTTGAGGATCAGAAGGA+TGG + tig0027765:6623-6642 None:intergenic 40.0%
! TCACTACTTTGAGGATCAGA+AGG + tig0027765:6627-6646 None:intergenic 40.0%
TCTGATCCTCAAAGTAGTGA+TGG - tig0027765:6627-6646 Msa1435620:three_prime_UTR 40.0%
TGAATGCCATCACTACTTTG+AGG + tig0027765:6636-6655 None:intergenic 40.0%
TTCTTCTGTGGAACCAAACA+AGG - tig0027765:6966-6985 Msa1435620:three_prime_UTR 40.0%
GAACCAAACAAGGCAACAAT+GGG - tig0027765:6976-6995 Msa1435620:three_prime_UTR 40.0%
! TGGGTTGGAAATAAAAGGTC+CGG - tig0027765:6995-7014 Msa1435620:three_prime_UTR 40.0%
AAGTTGGGGAACCCAAAATA+AGG - tig0027765:7029-7048 Msa1435620:three_prime_UTR 40.0%
! TTTTGCCCTTGCATGTTGTA+TGG - tig0027765:7145-7164 Msa1435620:CDS 40.0%
TGGGCGGTTAAAAATCAACA+CGG - tig0027765:7178-7197 Msa1435620:intron 40.0%
CGGAAATCAAGTAAGTGTAG+CGG - tig0027765:7198-7217 Msa1435620:intron 40.0%
GACACAATTAGAGAGAGAGT+TGG - tig0027765:7277-7296 Msa1435620:intron 40.0%
CACAATTAGAGAGAGAGTTG+GGG - tig0027765:7279-7298 Msa1435620:intron 40.0%
AGCACCTATAGTAGAAAGGA+TGG - tig0027765:7303-7322 Msa1435620:CDS 40.0%
GGATGGTAGAAAATAGGCTT+AGG - tig0027765:7320-7339 Msa1435620:CDS 40.0%
TGGTAGAAAATAGGCTTAGG+TGG - tig0027765:7323-7342 Msa1435620:CDS 40.0%
GAAAATAGGCTTAGGTGGTT+TGG - tig0027765:7328-7347 Msa1435620:CDS 40.0%
GTTGGGTCCAAATATGGTTT+CGG - tig0027765:7472-7491 Msa1435620:intron 40.0%
CGTGCTTATTCTCTTTGACT+AGG - tig0027765:7608-7627 Msa1435620:intron 40.0%
GTTAATTAGGATAGGAGGCA+GGG + tig0027765:7850-7869 None:intergenic 40.0%
GAGACGTTAATTAGGATAGG+AGG + tig0027765:7855-7874 None:intergenic 40.0%
! TATTCCCCTCTAGATTTTGC+AGG - tig0027765:8268-8287 Msa1435620:intron 40.0%
! TAGATTTTGCAGGAGAGGAA+AGG - tig0027765:8278-8297 Msa1435620:intron 40.0%
! AGTACTTACTTTGGTGCAGA+TGG + tig0027765:8466-8485 None:intergenic 40.0%
TGTAAATCGGATATCCTCTG+CGG - tig0027765:8499-8518 Msa1435620:intron 40.0%
!! GGTGTTATGTTTTCACTGGA+TGG - tig0027765:8603-8622 Msa1435620:intron 40.0%
GATCAAATAGTCTGCAATCC+AGG - tig0027765:8912-8931 Msa1435620:CDS 40.0%
GAATTGATCCTCTATAGTCC+TGG + tig0027765:8933-8952 None:intergenic 40.0%
! GAGCAGCAGTGCTATTGAAT+TGG - tig0027765:6545-6564 Msa1435620:three_prime_UTR 45.0%
AGGATGGTTTGACACGGAAA+TGG + tig0027765:6607-6626 None:intergenic 45.0%
! ATGTGGTTGCTTTCCGTTCA+TGG - tig0027765:6787-6806 Msa1435620:three_prime_UTR 45.0%
TGTATGCAGGTCTTCTTCTG+TGG - tig0027765:6954-6973 Msa1435620:three_prime_UTR 45.0%
GGAACCAAACAAGGCAACAA+TGG - tig0027765:6975-6994 Msa1435620:three_prime_UTR 45.0%
! CAACCCATTGTTGCCTTGTT+TGG + tig0027765:6982-7001 None:intergenic 45.0%
CAAACAAGGCAACAATGGGT+TGG - tig0027765:6980-6999 Msa1435620:three_prime_UTR 45.0%
GGGTTGGAAATAAAAGGTCC+GGG - tig0027765:6996-7015 Msa1435620:three_prime_UTR 45.0%
! TTGGGTTCCCCAACTTGTTT+CGG + tig0027765:7025-7044 None:intergenic 45.0%
GTTTCTGCATGAAGAGGCTT+AGG - tig0027765:7066-7085 Msa1435620:three_prime_UTR 45.0%
ATGAAGAGGCTTAGGTGGTT+TGG - tig0027765:7074-7093 Msa1435620:three_prime_UTR 45.0%
!! TAGGTGGTTTGGTCATTACC+AGG - tig0027765:7085-7104 Msa1435620:three_prime_UTR 45.0%
!! AGGTGGTTTGGTCATTACCA+GGG - tig0027765:7086-7105 Msa1435620:three_prime_UTR 45.0%
ATGCAATACTGTACTTGCCC+TGG + tig0027765:7106-7125 None:intergenic 45.0%
CCGTACCATACAACATGCAA+GGG + tig0027765:7153-7172 None:intergenic 45.0%
CCCTTGCATGTTGTATGGTA+CGG - tig0027765:7150-7169 Msa1435620:CDS 45.0%
TCCGTACCATACAACATGCA+AGG + tig0027765:7154-7173 None:intergenic 45.0%
TGTTGTATGGTACGGAGTGT+TGG - tig0027765:7158-7177 Msa1435620:CDS 45.0%
! GTTGTATGGTACGGAGTGTT+GGG - tig0027765:7159-7178 Msa1435620:CDS 45.0%
! AAGTAAGTGTAGCGGAGATG+AGG - tig0027765:7206-7225 Msa1435620:intron 45.0%
AGGATGTTGCGTTGGATGAT+TGG - tig0027765:7226-7245 Msa1435620:intron 45.0%
GGGTAGCACCTATAGTAGAA+AGG - tig0027765:7299-7318 Msa1435620:CDS 45.0%
GAGGAGAGTCTGATATGAAG+AGG - tig0027765:7403-7422 Msa1435620:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + tig0027765:7535-7554 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + tig0027765:7536-7555 None:intergenic 45.0%
CGTTAATTAGGATAGGAGGC+AGG + tig0027765:7851-7870 None:intergenic 45.0%
! GAGGGCATGTATGATTGAAC+TGG - tig0027765:8232-8251 Msa1435620:intron 45.0%
CTCTCCTGCAAAATCTAGAG+GGG + tig0027765:8275-8294 None:intergenic 45.0%
CCTCTCCTGCAAAATCTAGA+GGG + tig0027765:8276-8295 None:intergenic 45.0%
TCCTCTCCTGCAAAATCTAG+AGG + tig0027765:8277-8296 None:intergenic 45.0%
GTGAAGCTTGACTAAGCTGA+GGG + tig0027765:8533-8552 None:intergenic 45.0%
AGTGAAGCTTGACTAAGCTG+AGG + tig0027765:8534-8553 None:intergenic 45.0%
CTCATACTGTGACATTGGTG+AGG - tig0027765:8699-8718 Msa1435620:intron 45.0%
! CATTGGTGAGGCTTTGATGA+AGG - tig0027765:8711-8730 Msa1435620:CDS 45.0%
!!! GTTGTTCTGTTTTCAGGTGC+AGG - tig0027765:8745-8764 Msa1435620:CDS 45.0%
TTCCACAACTAAGGCAGTTG+CGG - tig0027765:8795-8814 Msa1435620:CDS 45.0%
TCTGCAATCCAGGACTATAG+AGG - tig0027765:8922-8941 Msa1435620:CDS 45.0%
CAACTTGGAGATCACCACTA+AGG + tig0027765:8967-8986 None:intergenic 45.0%
CTTAGTGGTGATCTCCAAGT+TGG - tig0027765:8965-8984 Msa1435620:CDS 45.0%
! TGGTGATCTCCAAGTTGGAA+TGG - tig0027765:8970-8989 Msa1435620:CDS 45.0%
! GGTGATCTCCAAGTTGGAAT+GGG - tig0027765:8971-8990 Msa1435620:CDS 45.0%
! GTGATCTCCAAGTTGGAATG+GGG - tig0027765:8972-8991 Msa1435620:CDS 45.0%
CAACAGAGCAGAGGTTGTCT+TGG - tig0027765:6411-6430 Msa1435620:three_prime_UTR 50.0%
TCTGCATGAAGAGGCTTAGG+TGG - tig0027765:7069-7088 Msa1435620:three_prime_UTR 50.0%
CTTATCCCACTAAGTGGGGT+CGG + tig0027765:7531-7550 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + tig0027765:7537-7556 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG - tig0027765:7534-7553 Msa1435620:intron 50.0%
GAGAATAAGCACGGGCAGTA+AGG + tig0027765:7601-7620 None:intergenic 50.0%
GAGGTGGAGGGAGTTAAACT+TGG - tig0027765:7780-7799 Msa1435620:intron 50.0%
CCACCTACGAGAGTCTACTT+GGG - tig0027765:8043-8062 Msa1435620:intron 50.0%
AGTCCCAAGTAGACTCTCGT+AGG + tig0027765:8049-8068 None:intergenic 50.0%
AGAGGAATGCGCTCAGGAAA+AGG + tig0027765:8078-8097 None:intergenic 50.0%
! ACTCTAAGAGGAATGCGCTC+AGG + tig0027765:8084-8103 None:intergenic 50.0%
TGCTCCCTAACCGTAACTGA+TGG - tig0027765:8155-8174 Msa1435620:intron 50.0%
! CCCTCTAGATTTTGCAGGAG+AGG - tig0027765:8273-8292 Msa1435620:intron 50.0%
TTTGCAGGAGAGGAAAGGTG+TGG - tig0027765:8283-8302 Msa1435620:intron 50.0%
ATCCGCAACTGCCTTAGTTG+TGG + tig0027765:8800-8819 None:intergenic 50.0%
! ATCTCCAAGTTGGAATGGGG+CGG - tig0027765:8975-8994 Msa1435620:CDS 50.0%
CCTCTGCTCTGTTGCAAAGC+AGG + tig0027765:6405-6424 None:intergenic 55.0%
CCTGCTTTGCAACAGAGCAG+AGG - tig0027765:6402-6421 Msa1435620:three_prime_UTR 55.0%
!! GTATGGTACGGAGTGTTGGG+CGG - tig0027765:7162-7181 Msa1435620:CDS 55.0%
CGGAGATGAGGATGTTGCGT+TGG - tig0027765:7218-7237 Msa1435620:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + tig0027765:7523-7542 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - tig0027765:7522-7541 Msa1435620:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - tig0027765:7523-7542 Msa1435620:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG - tig0027765:7529-7548 Msa1435620:intron 55.0%
TCGTAGGTGGCGTTCCAACT+AGG + tig0027765:8033-8052 None:intergenic 55.0%
GCCACCTACGAGAGTCTACT+TGG - tig0027765:8042-8061 Msa1435620:intron 55.0%
CCCAAGTAGACTCTCGTAGG+TGG + tig0027765:8046-8065 None:intergenic 55.0%
!! TGAGGGATTAGTCTCCGCAG+AGG + tig0027765:8516-8535 None:intergenic 55.0%
TTTCCTTCCAGTGCTGCCGT+AGG - tig0027765:8670-8689 Msa1435620:intron 55.0%
! ATTCCTACGGCAGCACTGGA+AGG + tig0027765:8676-8695 None:intergenic 55.0%
GATGATTCCTACGGCAGCAC+TGG + tig0027765:8680-8699 None:intergenic 55.0%
GGCGAGGCCGATGCATCTAA+GGG - tig0027765:6465-6484 Msa1435620:three_prime_UTR 60.0%
TCCGGGCGTCCGAAACAAGT+TGG - tig0027765:7013-7032 Msa1435620:three_prime_UTR 60.0%
! CCCAACTTGTTTCGGACGCC+CGG + tig0027765:7017-7036 None:intergenic 60.0%
CCGGGCGTCCGAAACAAGTT+GGG - tig0027765:7014-7033 Msa1435620:three_prime_UTR 60.0%
CGGGCGTCCGAAACAAGTTG+GGG - tig0027765:7015-7034 Msa1435620:three_prime_UTR 60.0%
GGGCGAGGCCGATGCATCTA+AGG - tig0027765:6464-6483 Msa1435620:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0027765 gene 6387 8998 6387 ID=Msa1435620;Name=Msa1435620
tig0027765 mRNA 6387 8998 6387 ID=Msa1435620-mRNA-1;Parent=Msa1435620;Name=Msa1435620-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|1|0.75|1|1|1|4|749|168
tig0027765 exon 6387 7189 6387 ID=Msa1435620-mRNA-1:exon:9816;Parent=Msa1435620-mRNA-1
tig0027765 exon 7284 7350 7284 ID=Msa1435620-mRNA-1:exon:9815;Parent=Msa1435620-mRNA-1
tig0027765 exon 8335 8422 8335 ID=Msa1435620-mRNA-1:exon:9814;Parent=Msa1435620-mRNA-1
tig0027765 exon 8701 8998 8701 ID=Msa1435620-mRNA-1:exon:9813;Parent=Msa1435620-mRNA-1
tig0027765 CDS 8701 8998 8701 ID=Msa1435620-mRNA-1:cds;Parent=Msa1435620-mRNA-1
tig0027765 CDS 8335 8422 8335 ID=Msa1435620-mRNA-1:cds;Parent=Msa1435620-mRNA-1
tig0027765 CDS 7284 7350 7284 ID=Msa1435620-mRNA-1:cds;Parent=Msa1435620-mRNA-1
tig0027765 CDS 7136 7189 7136 ID=Msa1435620-mRNA-1:cds;Parent=Msa1435620-mRNA-1
tig0027765 three_prime_UTR 6387 7135 6387 ID=Msa1435620-mRNA-1:three_prime_utr;Parent=Msa1435620-mRNA-1
Gene Sequence

>Msa1435620

ATGCTTCTGGTTAAACCTGCTTTGCAACAGAGCAGAGGTTGTCTTGGATGTTTCTCAAAACCTCTTGTTTTATTAATGGGCGAGGCCGATGCATCTAAGGGACTAAAAACTCAAGCCCAAACAATGAACAACGACAACAGATCTGAAGATTTTTTTTGGAGCAGCAGTGCTATTGAATTGGATCATGGTGCTATTCAGTCCCAGAGAAGCATGTCATCCATTTCCGTGTCAAACCATCCTTCTGATCCTCAAAGTAGTGATGGCATTCAGACTGATTCTCCAGAATTTGTCAATCATGGTCTTCTTCTGTGGAACCAAACAAGGCAACAATGGGTTGGAAATAAAAGGTCCGGGCGTCCGAAACAAGTTGGGGAACCCAAAATAAGTTGGAACGCCACCTACGAGAGTCTACTTGGGACTAACAAGCCTTTTCCTGAGCGCATTCCTCTTAGAGAAATGATTGACTTTCTTGTTGATATATGGGAATTAGAGGGCATGTATGATTGA
Protein sequence

>Msa1435620

MLLVKPALQQSRGCLGCFSKPLVLLMGEADASKGLKTQAQTMNNDNRSEDFFWSSSAIELDHGAIQSQRSMSSISVSNHPSDPQSSDGIQTDSPEFVNHGLLLWNQTRQQWVGNKRSGRPKQVGEPKISWNATYESLLGTNKPFPERIPLREMIDFLVDIWELEGMYD*