AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0432330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0432330 MtrunA17_Chr3g0137781 95.506 89 4 0 12 100 21 109 8.72e-49 153
Msa0432330 MtrunA17_Chr4g0063831 46.154 91 41 4 13 100 18 103 8.94e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0432330 AT5G25360.3 45.455 88 42 3 13 100 67 148 6.21e-16 70.9
Msa0432330 AT4G32342.1 41.053 95 45 3 7 100 10 94 3.84e-13 62.4
Msa0432330 AT4G32342.2 41.053 95 45 3 7 100 48 132 9.12e-13 62.0
Msa0432330 AT5G25360.2 45.000 80 37 3 21 100 29 101 4.30e-12 59.7
Msa0432330 AT5G25360.1 45.000 80 37 3 21 100 29 101 4.30e-12 59.7

Find 90 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATTTATTTACCTTATTT+TGG 0.062991 3_2:-84494846 None:intergenic
TTCATCCTCTTAATGTTTAT+TGG 0.159120 3_2:+84496115 Msa0432330:three_prime_UTR
TTGCCATGATTGACAAATTC+TGG 0.203050 3_2:-84494472 None:intergenic
TTGACTTTCTTGTTGATATA+TGG 0.216717 3_2:+84496010 Msa0432330:three_prime_UTR
CAACCCATTGTTGCCTTGTT+TGG 0.223693 3_2:-84494784 None:intergenic
GACACTTGTATTTATGATCT+TGG 0.235527 3_2:+84496432 Msa0432330:three_prime_UTR
GGGCGAGGCCGATGCATCTA+AGG 0.287521 3_2:+84494270 Msa0432330:CDS
AAATGGATGACATGCTTCTC+TGG 0.301324 3_2:-84494393 None:intergenic
TGTTGTCGTTGTTCATTGTT+TGG 0.311681 3_2:-84494309 None:intergenic
AACAATGGGTTGGAAATAAA+AGG 0.312465 3_2:+84494795 Msa0432330:exon
AGTGAAAACATAACACCTAT+TGG 0.318943 3_2:-84496404 None:intergenic
GGTTGTGTTAATCATGCTTC+TGG 0.347483 3_2:+84494180 None:intergenic
GAGCAGCAGTGCTATTGAAT+TGG 0.355690 3_2:+84494351 Msa0432330:CDS
TTGGGTTCCCCAACTTGTTT+CGG 0.356307 3_2:-84494827 None:intergenic
TATGATTGAACTGGCAAAAC+AGG 0.376802 3_2:+84496048 Msa0432330:three_prime_UTR
AACCAACTTAATAGAAAATC+AGG 0.378292 3_2:-84496237 None:intergenic
TGACTTTCTTGTTGATATAT+GGG 0.383956 3_2:+84496011 Msa0432330:three_prime_UTR
AAGTTGGGGAACCCAAAATA+AGG 0.384880 3_2:+84494834 Msa0432330:three_prime_UTR
TGTTGATATATGGGAATTAG+AGG 0.391346 3_2:+84496020 Msa0432330:three_prime_UTR
ACTATCCAATAAACATTAAG+AGG 0.394734 3_2:-84496120 None:intergenic
CCCAACTTGTTTCGGACGCC+CGG 0.401219 3_2:-84494819 None:intergenic
CCTCTGCTCTGTTGCAAAGC+AGG 0.407496 3_2:-84494208 None:intergenic
TCTCCAGAATTTGTCAATCA+TGG 0.416899 3_2:+84494469 Msa0432330:CDS
CCGGGCGTCCGAAACAAGTT+GGG 0.420971 3_2:+84494819 Msa0432330:three_prime_UTR
CAACAGAGCAGAGGTTGTCT+TGG 0.421310 3_2:+84494217 Msa0432330:CDS
GCCACCTACGAGAGTCTACT+TGG 0.426114 3_2:+84495849 Msa0432330:three_prime_UTR
AAAAGACTTAGTACTTACTT+TGG 0.426312 3_2:-84496279 None:intergenic
GTGAAAACATAACACCTATT+GGG 0.432741 3_2:-84496403 None:intergenic
CTGGAAGGAAATGTTAATCT+TGG 0.439465 3_2:-84496465 None:intergenic
GTTGTCGTTGTTCATTGTTT+GGG 0.444475 3_2:-84494308 None:intergenic
TGGGTTGGAAATAAAAGGTC+CGG 0.452762 3_2:+84494800 Msa0432330:exon
GGTGATCTCCAAGTTGGAAT+GGG 0.452928 3_2:+84496778 Msa0432330:three_prime_UTR
ATGAAAGAACTTACTCTAAG+AGG 0.460791 3_2:-84495900 None:intergenic
AGGATGGTTTGACACGGAAA+TGG 0.461819 3_2:-84494410 None:intergenic
AGAGGAATGCGCTCAGGAAA+AGG 0.472233 3_2:-84495882 None:intergenic
GATCAAATAGTCTGCAATCC+AGG 0.473644 3_2:+84496719 Msa0432330:three_prime_UTR
TGAATGCCATCACTACTTTG+AGG 0.479975 3_2:-84494439 None:intergenic
GAGGGCATGTATGATTGAAC+TGG 0.485460 3_2:+84496039 Msa0432330:three_prime_UTR
GATGATTCCTACGGCAGCAC+TGG 0.489025 3_2:-84496484 None:intergenic
TCCGGGCGTCCGAAACAAGT+TGG 0.490712 3_2:+84494818 Msa0432330:three_prime_UTR
ATCATCTCATACTGTGACAT+TGG 0.493232 3_2:+84496501 Msa0432330:three_prime_UTR
TTATTAATAACTGACCCAAT+AGG 0.501130 3_2:+84496389 Msa0432330:three_prime_UTR
TGGTGATCTCCAAGTTGGAA+TGG 0.502647 3_2:+84496777 Msa0432330:three_prime_UTR
GAACCAAACAAGGCAACAAT+GGG 0.503294 3_2:+84494781 Msa0432330:CDS
CTTAGTGGTGATCTCCAAGT+TGG 0.503397 3_2:+84496772 Msa0432330:three_prime_UTR
TTCTTCTGTGGAACCAAACA+AGG 0.508337 3_2:+84494771 Msa0432330:CDS
GAAGGAAATGTTAATCTTGG+AGG 0.512478 3_2:-84496462 None:intergenic
AGTACTTACTTTGGTGCAGA+TGG 0.516361 3_2:-84496270 None:intergenic
TCTGCAATCCAGGACTATAG+AGG 0.524169 3_2:+84496729 Msa0432330:three_prime_UTR
CATTGGTGAGGCTTTGATGA+AGG 0.524390 3_2:+84496518 Msa0432330:three_prime_UTR
ACTCTAAGAGGAATGCGCTC+AGG 0.532544 3_2:-84495888 None:intergenic
TTCCACAACTAAGGCAGTTG+CGG 0.533016 3_2:+84496602 Msa0432330:three_prime_UTR
GAATTGATCCTCTATAGTCC+TGG 0.533065 3_2:-84496737 None:intergenic
TACTTTGAGGATCAGAAGGA+TGG 0.543246 3_2:-84494426 None:intergenic
GCTCCCGCCCCATTCCAACT+TGG 0.549677 3_2:-84496786 None:intergenic
GGAACCAAACAAGGCAACAA+TGG 0.556597 3_2:+84494780 Msa0432330:CDS
AATGGATGACATGCTTCTCT+GGG 0.557186 3_2:-84494392 None:intergenic
ATCCGCAACTGCCTTAGTTG+TGG 0.558439 3_2:-84496604 None:intergenic
GGCGAGGCCGATGCATCTAA+GGG 0.563203 3_2:+84494271 Msa0432330:CDS
TCTCCAAGTTGGAATGGGGC+GGG 0.563451 3_2:+84496783 Msa0432330:three_prime_UTR
TGTATGCAGGTCTTCTTCTG+TGG 0.571627 3_2:+84494759 Msa0432330:intron
TCCTCTCCTGCAAAATCTAG+AGG 0.574443 3_2:-84496081 None:intergenic
CCTCTCCTGCAAAATCTAGA+GGG 0.578403 3_2:-84496080 None:intergenic
TCTGATCCTCAAAGTAGTGA+TGG 0.580218 3_2:+84494433 Msa0432330:CDS
ATTCCTACGGCAGCACTGGA+AGG 0.586917 3_2:-84496480 None:intergenic
CAAACAAGGCAACAATGGGT+TGG 0.588867 3_2:+84494785 Msa0432330:CDS
GGGTTGGAAATAAAAGGTCC+GGG 0.591087 3_2:+84494801 Msa0432330:exon
ATCTCCAAGTTGGAATGGGG+CGG 0.592965 3_2:+84496782 Msa0432330:three_prime_UTR
GTTGATATATGGGAATTAGA+GGG 0.593196 3_2:+84496021 Msa0432330:three_prime_UTR
TCGCCCATTAATAAAACAAG+AGG 0.595108 3_2:-84494253 None:intergenic
TCACTACTTTGAGGATCAGA+AGG 0.595919 3_2:-84494430 None:intergenic
CAACTTGGAGATCACCACTA+AGG 0.598538 3_2:-84496771 None:intergenic
TTTGCAGGAGAGGAAAGGTG+TGG 0.612135 3_2:+84496090 Msa0432330:three_prime_UTR
ACTATTTGATCAAAATAAGA+CGG 0.613083 3_2:-84496707 None:intergenic
TTTCCTTCCAGTGCTGCCGT+AGG 0.617308 3_2:+84496477 Msa0432330:three_prime_UTR
AGTGCTATTGAATTGGATCA+TGG 0.626780 3_2:+84494358 Msa0432330:CDS
ACAGTATGAGATGATTCCTA+CGG 0.628284 3_2:-84496493 None:intergenic
CTCATACTGTGACATTGGTG+AGG 0.628731 3_2:+84496506 Msa0432330:three_prime_UTR
CCTGCTTTGCAACAGAGCAG+AGG 0.631103 3_2:+84494208 Msa0432330:CDS
AGTGAAGCTTGACTAAGCTG+AGG 0.634103 3_2:-84496338 None:intergenic
GTGATCTCCAAGTTGGAATG+GGG 0.637300 3_2:+84496779 Msa0432330:three_prime_UTR
GTGAAGCTTGACTAAGCTGA+GGG 0.640473 3_2:-84496337 None:intergenic
CTCTCCTGCAAAATCTAGAG+GGG 0.644947 3_2:-84496079 None:intergenic
CCCAAGTAGACTCTCGTAGG+TGG 0.647046 3_2:-84495850 None:intergenic
ATCAGAAGGATGGTTTGACA+CGG 0.658576 3_2:-84494416 None:intergenic
CGGGCGTCCGAAACAAGTTG+GGG 0.664193 3_2:+84494820 Msa0432330:three_prime_UTR
AGTCCCAAGTAGACTCTCGT+AGG 0.676556 3_2:-84495853 None:intergenic
CCACCTACGAGAGTCTACTT+GGG 0.677939 3_2:+84495850 Msa0432330:three_prime_UTR
TGAGGGATTAGTCTCCGCAG+AGG 0.727780 3_2:-84496320 None:intergenic
TGTAAATCGGATATCCTCTG+CGG 0.750038 3_2:+84496306 Msa0432330:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTATTTACCTTATTTT+GGG - chr3_2:84494848-84494867 None:intergenic 10.0%
!!! TATATTTATTTACCTTATTT+TGG - chr3_2:84494849-84494868 None:intergenic 10.0%
!! AAATAAGGTAAATAAATATA+AGG + chr3_2:84494849-84494868 Msa0432330:intron 10.0%
!!! TTTTTTTTTCTGATTATAAT+TGG + chr3_2:84495756-84495775 Msa0432330:intron 10.0%
!!! TTTTTTTTCTGATTATAATT+GGG + chr3_2:84495757-84495776 Msa0432330:intron 10.0%
!! AAAGAAAATATACAAATAGA+GGG - chr3_2:84494532-84494551 None:intergenic 15.0%
!! AAAAGAAAATATACAAATAG+AGG - chr3_2:84494533-84494552 None:intergenic 15.0%
!! CAAAATATAATAAAAAGACA+AGG - chr3_2:84494698-84494717 None:intergenic 15.0%
!!! AAAACCTCTTGTTTTATTAA+TGG + chr3_2:84494249-84494268 Msa0432330:CDS 20.0%
!!! AAACCTCTTGTTTTATTAAT+GGG + chr3_2:84494250-84494269 Msa0432330:CDS 20.0%
!! AAGAAAATATACAAATAGAG+GGG - chr3_2:84494531-84494550 None:intergenic 20.0%
!!! TTTTGTTTCTTTTATCTTGA+TGG + chr3_2:84494556-84494575 Msa0432330:intron 20.0%
!! ATTATTACAAGCAATTGATA+AGG - chr3_2:84494727-84494746 None:intergenic 20.0%
!! TTATCAATTGCTTGTAATAA+TGG + chr3_2:84494726-84494745 Msa0432330:intron 20.0%
!! TAAAAAATGTATGAGTGATA+AGG + chr3_2:84495525-84495544 Msa0432330:intron 20.0%
!!! TTTTGTCAAGTTCTAATTTT+CGG + chr3_2:84495805-84495824 Msa0432330:intron 20.0%
!!! TTGTTTTTATTTTTTCTGTG+TGG + chr3_2:84496167-84496186 Msa0432330:three_prime_UTR 20.0%
!! ACTATTTGATCAAAATAAGA+CGG - chr3_2:84496710-84496729 None:intergenic 20.0%
!!! ACAGATCTGAAGATTTTTTT+TGG + chr3_2:84494329-84494348 Msa0432330:CDS 25.0%
!! TTAACTTTAAGAACCATGAA+CGG - chr3_2:84494610-84494629 None:intergenic 25.0%
!!! CTTTCTTCTATCGATTTTAT+AGG + chr3_2:84494668-84494687 Msa0432330:intron 25.0%
! CTTTGTATTACAACATCTAT+CGG - chr3_2:84495177-84495196 None:intergenic 25.0%
! AATACAAAGAGTAGATAAGA+TGG + chr3_2:84495188-84495207 Msa0432330:intron 25.0%
! AATCTTTCCTAATAGTTTCT+AGG - chr3_2:84495246-84495265 None:intergenic 25.0%
! AACTATTAGGAAAGATTTAG+AGG + chr3_2:84495249-84495268 Msa0432330:intron 25.0%
! GGAAAAAAATGTACAAACAA+GGG - chr3_2:84495387-84495406 None:intergenic 25.0%
! AGGAAAAAAATGTACAAACA+AGG - chr3_2:84495388-84495407 None:intergenic 25.0%
! TGGTCTAAACAATGAATAAA+AGG - chr3_2:84495484-84495503 None:intergenic 25.0%
!! CTTGAAGCAAAAGATTTTTA+TGG - chr3_2:84495504-84495523 None:intergenic 25.0%
! AAGTAGAGAAATTAATTGAG+AGG + chr3_2:84495568-84495587 Msa0432330:intron 25.0%
!! TTTTCCAAAGATAGAATCAT+GGG - chr3_2:84495692-84495711 None:intergenic 25.0%
!! ATTTTCCAAAGATAGAATCA+TGG - chr3_2:84495693-84495712 None:intergenic 25.0%
!! TTCTTTATCTACTCATTTTC+TGG + chr3_2:84495933-84495952 Msa0432330:intron 25.0%
! AATGATATTACCATCAGTTA+CGG - chr3_2:84495975-84495994 None:intergenic 25.0%
! TTGACTTTCTTGTTGATATA+TGG + chr3_2:84496010-84496029 Msa0432330:three_prime_UTR 25.0%
! TGACTTTCTTGTTGATATAT+GGG + chr3_2:84496011-84496030 Msa0432330:three_prime_UTR 25.0%
! TTCATCCTCTTAATGTTTAT+TGG + chr3_2:84496115-84496134 Msa0432330:three_prime_UTR 25.0%
! ACTATCCAATAAACATTAAG+AGG - chr3_2:84496123-84496142 None:intergenic 25.0%
!!! TTTTTATTTTTTCTGTGTGG+AGG + chr3_2:84496170-84496189 Msa0432330:three_prime_UTR 25.0%
!! TTCCTGATTTTCTATTAAGT+TGG + chr3_2:84496235-84496254 Msa0432330:three_prime_UTR 25.0%
! AACCAACTTAATAGAAAATC+AGG - chr3_2:84496240-84496259 None:intergenic 25.0%
! AAAAGACTTAGTACTTACTT+TGG - chr3_2:84496282-84496301 None:intergenic 25.0%
!! AAGTCTTTTTGTGTGTAAAT+CGG + chr3_2:84496293-84496312 Msa0432330:three_prime_UTR 25.0%
! TTATTAATAACTGACCCAAT+AGG + chr3_2:84496389-84496408 Msa0432330:three_prime_UTR 25.0%
!!! TTCTTTATTTTCTACCTTAG+TGG + chr3_2:84496757-84496776 Msa0432330:three_prime_UTR 25.0%
! GGAATCGCTTTAAATATATG+TGG + chr3_2:84494577-84494596 Msa0432330:intron 30.0%
TTACAAGCAATTGATAAGGA+TGG - chr3_2:84494723-84494742 None:intergenic 30.0%
!! AACAATGGGTTGGAAATAAA+AGG + chr3_2:84494795-84494814 Msa0432330:exon 30.0%
TAGAAAGGATGGTAGAAAAT+AGG + chr3_2:84495121-84495140 Msa0432330:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG + chr3_2:84495261-84495280 Msa0432330:intron 30.0%
GATTTAGAGGTTAATGAGTT+GGG + chr3_2:84495262-84495281 Msa0432330:intron 30.0%
GTTATATCCGAAACCATATT+TGG - chr3_2:84495289-84495308 None:intergenic 30.0%
TGGTTTCGGATATAACATTA+TGG + chr3_2:84495293-84495312 Msa0432330:intron 30.0%
TAGAGAAATTAATTGAGAGG+TGG + chr3_2:84495571-84495590 Msa0432330:intron 30.0%
GAAATTAGGAGACGTTAATT+AGG - chr3_2:84495670-84495689 None:intergenic 30.0%
AGATAGAATCATGGGAAATT+AGG - chr3_2:84495684-84495703 None:intergenic 30.0%
ATTTCCCATGATTCTATCTT+TGG + chr3_2:84495685-84495704 Msa0432330:intron 30.0%
ATGAAAGAACTTACTCTAAG+AGG - chr3_2:84495903-84495922 None:intergenic 30.0%
TGTTGATATATGGGAATTAG+AGG + chr3_2:84496020-84496039 Msa0432330:three_prime_UTR 30.0%
GTTGATATATGGGAATTAGA+GGG + chr3_2:84496021-84496040 Msa0432330:three_prime_UTR 30.0%
!!! TTTTTTAAATAGCAGCGCTT+AGG - chr3_2:84496364-84496383 None:intergenic 30.0%
GTGAAAACATAACACCTATT+GGG - chr3_2:84496406-84496425 None:intergenic 30.0%
AGTGAAAACATAACACCTAT+TGG - chr3_2:84496407-84496426 None:intergenic 30.0%
!! AATAGGTGTTATGTTTTCAC+TGG + chr3_2:84496406-84496425 Msa0432330:three_prime_UTR 30.0%
GACACTTGTATTTATGATCT+TGG + chr3_2:84496432-84496451 Msa0432330:three_prime_UTR 30.0%
!!! GTGTTTTTTTTCCACAACTA+AGG + chr3_2:84496593-84496612 Msa0432330:three_prime_UTR 30.0%
TCGCCCATTAATAAAACAAG+AGG - chr3_2:84494256-84494275 None:intergenic 35.0%
!!! TCTTGTTTTATTAATGGGCG+AGG + chr3_2:84494255-84494274 Msa0432330:CDS 35.0%
! TTTTAGTCCCTTAGATGCAT+CGG - chr3_2:84494281-84494300 None:intergenic 35.0%
GTTGTCGTTGTTCATTGTTT+GGG - chr3_2:84494311-84494330 None:intergenic 35.0%
TGTTGTCGTTGTTCATTGTT+TGG - chr3_2:84494312-84494331 None:intergenic 35.0%
! AGTGCTATTGAATTGGATCA+TGG + chr3_2:84494358-84494377 Msa0432330:CDS 35.0%
TCTCCAGAATTTGTCAATCA+TGG + chr3_2:84494469-84494488 Msa0432330:CDS 35.0%
TTGCCATGATTGACAAATTC+TGG - chr3_2:84494475-84494494 None:intergenic 35.0%
AGGGGTGATAATAGATAAAC+TGG - chr3_2:84494513-84494532 None:intergenic 35.0%
!! TGGTAAGTTTTGATGTATGC+AGG + chr3_2:84494746-84494765 Msa0432330:intron 35.0%
TATAAGGTTTCTGCATGAAG+AGG + chr3_2:84494865-84494884 Msa0432330:intron 35.0%
CAAGGGCAAAAATGATCAAA+TGG - chr3_2:84494941-84494960 None:intergenic 35.0%
TTGGTAAGACTAGACTAGAT+AGG + chr3_2:84495050-84495069 Msa0432330:intron 35.0%
AGACTAGACTAGATAGGATT+AGG + chr3_2:84495056-84495075 Msa0432330:intron 35.0%
ACAATTAGAGAGAGAGAGTT+GGG + chr3_2:84495085-84495104 Msa0432330:intron 35.0%
TCTACCATCCTTTCTACTAT+AGG - chr3_2:84495117-84495136 None:intergenic 35.0%
AAAATAGGCTTAGGTGGTTT+GGG + chr3_2:84495136-84495155 Msa0432330:intron 35.0%
ACAAAGAGTAGATAAGATGG+AGG + chr3_2:84495191-84495210 Msa0432330:intron 35.0%
AGGAAGACCTAGAAACTATT+AGG + chr3_2:84495236-84495255 Msa0432330:intron 35.0%
! TAATGAGTTGGGTCCAAATA+TGG + chr3_2:84495273-84495292 Msa0432330:intron 35.0%
TAGTCAAAGAGAATAAGCAC+GGG - chr3_2:84495416-84495435 None:intergenic 35.0%
CTAGTCAAAGAGAATAAGCA+CGG - chr3_2:84495417-84495436 None:intergenic 35.0%
AGAAATTAATTGAGAGGTGG+AGG + chr3_2:84495574-84495593 Msa0432330:intron 35.0%
GAAATTAATTGAGAGGTGGA+GGG + chr3_2:84495575-84495594 Msa0432330:intron 35.0%
TAGGAGACGTTAATTAGGAT+AGG - chr3_2:84495665-84495684 None:intergenic 35.0%
TGATTATAATTGGGACCAGA+AGG + chr3_2:84495766-84495785 Msa0432330:intron 35.0%
! ATCTTAAAAAGTACGCCTTC+TGG - chr3_2:84495784-84495803 None:intergenic 35.0%
TTCGGATTTATGTTCCTAGT+TGG + chr3_2:84495823-84495842 Msa0432330:intron 35.0%
ATTACCATCAGTTACGGTTA+GGG - chr3_2:84495969-84495988 None:intergenic 35.0%
TATTACCATCAGTTACGGTT+AGG - chr3_2:84495970-84495989 None:intergenic 35.0%
ATGGTAATATCATTCGTGAC+AGG + chr3_2:84495981-84496000 Msa0432330:intron 35.0%
TATGATTGAACTGGCAAAAC+AGG + chr3_2:84496048-84496067 Msa0432330:three_prime_UTR 35.0%
GAAGGAAATGTTAATCTTGG+AGG - chr3_2:84496465-84496484 None:intergenic 35.0%
CTGGAAGGAAATGTTAATCT+TGG - chr3_2:84496468-84496487 None:intergenic 35.0%
ACAGTATGAGATGATTCCTA+CGG - chr3_2:84496496-84496515 None:intergenic 35.0%
ATCATCTCATACTGTGACAT+TGG + chr3_2:84496501-84496520 Msa0432330:three_prime_UTR 35.0%
! CACAAAGTTGTTCTGTTTTC+AGG + chr3_2:84496546-84496565 Msa0432330:three_prime_UTR 35.0%
AATGGATGACATGCTTCTCT+GGG - chr3_2:84494395-84494414 None:intergenic 40.0%
AAATGGATGACATGCTTCTC+TGG - chr3_2:84494396-84494415 None:intergenic 40.0%
ATCAGAAGGATGGTTTGACA+CGG - chr3_2:84494419-84494438 None:intergenic 40.0%
! TACTTTGAGGATCAGAAGGA+TGG - chr3_2:84494429-84494448 None:intergenic 40.0%
! TCACTACTTTGAGGATCAGA+AGG - chr3_2:84494433-84494452 None:intergenic 40.0%
TCTGATCCTCAAAGTAGTGA+TGG + chr3_2:84494433-84494452 Msa0432330:CDS 40.0%
TGAATGCCATCACTACTTTG+AGG - chr3_2:84494442-84494461 None:intergenic 40.0%
TTCTTCTGTGGAACCAAACA+AGG + chr3_2:84494771-84494790 Msa0432330:CDS 40.0%
GAACCAAACAAGGCAACAAT+GGG + chr3_2:84494781-84494800 Msa0432330:CDS 40.0%
! TGGGTTGGAAATAAAAGGTC+CGG + chr3_2:84494800-84494819 Msa0432330:exon 40.0%
AAGTTGGGGAACCCAAAATA+AGG + chr3_2:84494834-84494853 Msa0432330:three_prime_UTR 40.0%
! TTTTGCCCTTGCATGTTGTA+TGG + chr3_2:84494950-84494969 Msa0432330:intron 40.0%
TGGGCGGTTAAAAATCAACA+CGG + chr3_2:84494983-84495002 Msa0432330:intron 40.0%
CGGAAATCAAGTAAGTGTAG+CGG + chr3_2:84495003-84495022 Msa0432330:intron 40.0%
CACAATTAGAGAGAGAGAGT+TGG + chr3_2:84495084-84495103 Msa0432330:intron 40.0%
CAATTAGAGAGAGAGAGTTG+GGG + chr3_2:84495086-84495105 Msa0432330:intron 40.0%
AGCACCTATAGTAGAAAGGA+TGG + chr3_2:84495110-84495129 Msa0432330:intron 40.0%
GGATGGTAGAAAATAGGCTT+AGG + chr3_2:84495127-84495146 Msa0432330:intron 40.0%
TGGTAGAAAATAGGCTTAGG+TGG + chr3_2:84495130-84495149 Msa0432330:intron 40.0%
GAAAATAGGCTTAGGTGGTT+TGG + chr3_2:84495135-84495154 Msa0432330:intron 40.0%
AGTCTGATATGAAGAGGTAG+AGG + chr3_2:84495216-84495235 Msa0432330:intron 40.0%
GTTGGGTCCAAATATGGTTT+CGG + chr3_2:84495279-84495298 Msa0432330:intron 40.0%
CGTGCTTATTCTCTTTGACT+AGG + chr3_2:84495415-84495434 Msa0432330:intron 40.0%
GTTAATTAGGATAGGAGGCA+GGG - chr3_2:84495657-84495676 None:intergenic 40.0%
GAGACGTTAATTAGGATAGG+AGG - chr3_2:84495662-84495681 None:intergenic 40.0%
! TATTCCCCTCTAGATTTTGC+AGG + chr3_2:84496075-84496094 Msa0432330:three_prime_UTR 40.0%
! TAGATTTTGCAGGAGAGGAA+AGG + chr3_2:84496085-84496104 Msa0432330:three_prime_UTR 40.0%
! AGTACTTACTTTGGTGCAGA+TGG - chr3_2:84496273-84496292 None:intergenic 40.0%
TGTAAATCGGATATCCTCTG+CGG + chr3_2:84496306-84496325 Msa0432330:three_prime_UTR 40.0%
GATCAAATAGTCTGCAATCC+AGG + chr3_2:84496719-84496738 Msa0432330:three_prime_UTR 40.0%
GAATTGATCCTCTATAGTCC+TGG - chr3_2:84496740-84496759 None:intergenic 40.0%
! GAGCAGCAGTGCTATTGAAT+TGG + chr3_2:84494351-84494370 Msa0432330:CDS 45.0%
AGGATGGTTTGACACGGAAA+TGG - chr3_2:84494413-84494432 None:intergenic 45.0%
! ATGTGGTTGCTTTCCGTTCA+TGG + chr3_2:84494594-84494613 Msa0432330:intron 45.0%
TGTATGCAGGTCTTCTTCTG+TGG + chr3_2:84494759-84494778 Msa0432330:intron 45.0%
GGAACCAAACAAGGCAACAA+TGG + chr3_2:84494780-84494799 Msa0432330:CDS 45.0%
! CAACCCATTGTTGCCTTGTT+TGG - chr3_2:84494787-84494806 None:intergenic 45.0%
CAAACAAGGCAACAATGGGT+TGG + chr3_2:84494785-84494804 Msa0432330:CDS 45.0%
GGGTTGGAAATAAAAGGTCC+GGG + chr3_2:84494801-84494820 Msa0432330:exon 45.0%
! TTGGGTTCCCCAACTTGTTT+CGG - chr3_2:84494830-84494849 None:intergenic 45.0%
GTTTCTGCATGAAGAGGCTT+AGG + chr3_2:84494871-84494890 Msa0432330:intron 45.0%
ATGAAGAGGCTTAGGTGGTT+TGG + chr3_2:84494879-84494898 Msa0432330:intron 45.0%
!! TAGGTGGTTTGGTCATTACC+AGG + chr3_2:84494890-84494909 Msa0432330:intron 45.0%
!! AGGTGGTTTGGTCATTACCA+GGG + chr3_2:84494891-84494910 Msa0432330:intron 45.0%
ATGCAATACTGTACTTGCCC+TGG - chr3_2:84494911-84494930 None:intergenic 45.0%
CCGTACCATACAACATGCAA+GGG - chr3_2:84494958-84494977 None:intergenic 45.0%
CCCTTGCATGTTGTATGGTA+CGG + chr3_2:84494955-84494974 Msa0432330:intron 45.0%
TCCGTACCATACAACATGCA+AGG - chr3_2:84494959-84494978 None:intergenic 45.0%
TGTTGTATGGTACGGAGTGT+TGG + chr3_2:84494963-84494982 Msa0432330:intron 45.0%
! GTTGTATGGTACGGAGTGTT+GGG + chr3_2:84494964-84494983 Msa0432330:intron 45.0%
! AAGTAAGTGTAGCGGAGATG+AGG + chr3_2:84495011-84495030 Msa0432330:intron 45.0%
AGGATGTTGCGTTGGATGAT+TGG + chr3_2:84495031-84495050 Msa0432330:intron 45.0%
GGGTAGCACCTATAGTAGAA+AGG + chr3_2:84495106-84495125 Msa0432330:intron 45.0%
GAGGAGAGTCTGATATGAAG+AGG + chr3_2:84495210-84495229 Msa0432330:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr3_2:84495342-84495361 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr3_2:84495343-84495362 None:intergenic 45.0%
CGTTAATTAGGATAGGAGGC+AGG - chr3_2:84495658-84495677 None:intergenic 45.0%
! GAGGGCATGTATGATTGAAC+TGG + chr3_2:84496039-84496058 Msa0432330:three_prime_UTR 45.0%
CTCTCCTGCAAAATCTAGAG+GGG - chr3_2:84496082-84496101 None:intergenic 45.0%
CCTCTCCTGCAAAATCTAGA+GGG - chr3_2:84496083-84496102 None:intergenic 45.0%
TCCTCTCCTGCAAAATCTAG+AGG - chr3_2:84496084-84496103 None:intergenic 45.0%
GTGAAGCTTGACTAAGCTGA+GGG - chr3_2:84496340-84496359 None:intergenic 45.0%
AGTGAAGCTTGACTAAGCTG+AGG - chr3_2:84496341-84496360 None:intergenic 45.0%
CTCATACTGTGACATTGGTG+AGG + chr3_2:84496506-84496525 Msa0432330:three_prime_UTR 45.0%
! CATTGGTGAGGCTTTGATGA+AGG + chr3_2:84496518-84496537 Msa0432330:three_prime_UTR 45.0%
!!! GTTGTTCTGTTTTCAGGTGC+AGG + chr3_2:84496552-84496571 Msa0432330:three_prime_UTR 45.0%
TTCCACAACTAAGGCAGTTG+CGG + chr3_2:84496602-84496621 Msa0432330:three_prime_UTR 45.0%
TCTGCAATCCAGGACTATAG+AGG + chr3_2:84496729-84496748 Msa0432330:three_prime_UTR 45.0%
CAACTTGGAGATCACCACTA+AGG - chr3_2:84496774-84496793 None:intergenic 45.0%
CTTAGTGGTGATCTCCAAGT+TGG + chr3_2:84496772-84496791 Msa0432330:three_prime_UTR 45.0%
! TGGTGATCTCCAAGTTGGAA+TGG + chr3_2:84496777-84496796 Msa0432330:three_prime_UTR 45.0%
! GGTGATCTCCAAGTTGGAAT+GGG + chr3_2:84496778-84496797 Msa0432330:three_prime_UTR 45.0%
! GTGATCTCCAAGTTGGAATG+GGG + chr3_2:84496779-84496798 Msa0432330:three_prime_UTR 45.0%
CAACAGAGCAGAGGTTGTCT+TGG + chr3_2:84494217-84494236 Msa0432330:CDS 50.0%
TCTGCATGAAGAGGCTTAGG+TGG + chr3_2:84494874-84494893 Msa0432330:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - chr3_2:84495338-84495357 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr3_2:84495344-84495363 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + chr3_2:84495341-84495360 Msa0432330:intron 50.0%
GAGAATAAGCACGGGCAGTA+AGG - chr3_2:84495408-84495427 None:intergenic 50.0%
GAGGTGGAGGGAGTTAAACT+TGG + chr3_2:84495587-84495606 Msa0432330:intron 50.0%
CCACCTACGAGAGTCTACTT+GGG + chr3_2:84495850-84495869 Msa0432330:three_prime_UTR 50.0%
AGTCCCAAGTAGACTCTCGT+AGG - chr3_2:84495856-84495875 None:intergenic 50.0%
AGAGGAATGCGCTCAGGAAA+AGG - chr3_2:84495885-84495904 None:intergenic 50.0%
! ACTCTAAGAGGAATGCGCTC+AGG - chr3_2:84495891-84495910 None:intergenic 50.0%
TGCTCCCTAACCGTAACTGA+TGG + chr3_2:84495962-84495981 Msa0432330:intron 50.0%
! CCCTCTAGATTTTGCAGGAG+AGG + chr3_2:84496080-84496099 Msa0432330:three_prime_UTR 50.0%
TTTGCAGGAGAGGAAAGGTG+TGG + chr3_2:84496090-84496109 Msa0432330:three_prime_UTR 50.0%
ATCCGCAACTGCCTTAGTTG+TGG - chr3_2:84496607-84496626 None:intergenic 50.0%
! ATCTCCAAGTTGGAATGGGG+CGG + chr3_2:84496782-84496801 Msa0432330:three_prime_UTR 50.0%
CCTCTGCTCTGTTGCAAAGC+AGG - chr3_2:84494211-84494230 None:intergenic 55.0%
CCTGCTTTGCAACAGAGCAG+AGG + chr3_2:84494208-84494227 Msa0432330:CDS 55.0%
!! GTATGGTACGGAGTGTTGGG+CGG + chr3_2:84494967-84494986 Msa0432330:intron 55.0%
CGGAGATGAGGATGTTGCGT+TGG + chr3_2:84495023-84495042 Msa0432330:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr3_2:84495330-84495349 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG + chr3_2:84495329-84495348 Msa0432330:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG + chr3_2:84495330-84495349 Msa0432330:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG + chr3_2:84495336-84495355 Msa0432330:intron 55.0%
TCGTAGGTGGCGTTCCAACT+AGG - chr3_2:84495840-84495859 None:intergenic 55.0%
GCCACCTACGAGAGTCTACT+TGG + chr3_2:84495849-84495868 Msa0432330:three_prime_UTR 55.0%
CCCAAGTAGACTCTCGTAGG+TGG - chr3_2:84495853-84495872 None:intergenic 55.0%
!! TGAGGGATTAGTCTCCGCAG+AGG - chr3_2:84496323-84496342 None:intergenic 55.0%
TTTCCTTCCAGTGCTGCCGT+AGG + chr3_2:84496477-84496496 Msa0432330:three_prime_UTR 55.0%
! ATTCCTACGGCAGCACTGGA+AGG - chr3_2:84496483-84496502 None:intergenic 55.0%
GATGATTCCTACGGCAGCAC+TGG - chr3_2:84496487-84496506 None:intergenic 55.0%
GGCGAGGCCGATGCATCTAA+GGG + chr3_2:84494271-84494290 Msa0432330:CDS 60.0%
TCCGGGCGTCCGAAACAAGT+TGG + chr3_2:84494818-84494837 Msa0432330:three_prime_UTR 60.0%
! CCCAACTTGTTTCGGACGCC+CGG - chr3_2:84494822-84494841 None:intergenic 60.0%
CCGGGCGTCCGAAACAAGTT+GGG + chr3_2:84494819-84494838 Msa0432330:three_prime_UTR 60.0%
CGGGCGTCCGAAACAAGTTG+GGG + chr3_2:84494820-84494839 Msa0432330:three_prime_UTR 60.0%
GGGCGAGGCCGATGCATCTA+AGG + chr3_2:84494270-84494289 Msa0432330:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 84494193 84496805 84494193 ID=Msa0432330;Name=Msa0432330
chr3_2 mRNA 84494193 84496805 84494193 ID=Msa0432330-mRNA-1;Parent=Msa0432330;Name=Msa0432330-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.66|0.75|1|0.66|0.75|4|912|115
chr3_2 exon 84494193 84494494 84494193 ID=Msa0432330-mRNA-1:exon:23903;Parent=Msa0432330-mRNA-1
chr3_2 exon 84494768 84494855 84494768 ID=Msa0432330-mRNA-1:exon:23904;Parent=Msa0432330-mRNA-1
chr3_2 exon 84495842 84495908 84495842 ID=Msa0432330-mRNA-1:exon:23905;Parent=Msa0432330-mRNA-1
chr3_2 exon 84496003 84496805 84496003 ID=Msa0432330-mRNA-1:exon:23906;Parent=Msa0432330-mRNA-1
chr3_2 CDS 84494193 84494494 84494193 ID=Msa0432330-mRNA-1:cds;Parent=Msa0432330-mRNA-1
chr3_2 CDS 84494768 84494813 84494768 ID=Msa0432330-mRNA-1:cds;Parent=Msa0432330-mRNA-1
chr3_2 three_prime_UTR 84494814 84494855 84494814 ID=Msa0432330-mRNA-1:three_prime_utr;Parent=Msa0432330-mRNA-1
chr3_2 three_prime_UTR 84495842 84495908 84495842 ID=Msa0432330-mRNA-1:three_prime_utr;Parent=Msa0432330-mRNA-1
chr3_2 three_prime_UTR 84496003 84496805 84496003 ID=Msa0432330-mRNA-1:three_prime_utr;Parent=Msa0432330-mRNA-1
Gene Sequence

>Msa0432330

ATGCTTCTGGTTAAACCTGCTTTGCAACAGAGCAGAGGTTGTCTTGGATGTTTCTCAAAACCTCTTGTTTTATTAATGGGCGAGGCCGATGCATCTAAGGGACTAAAAACTCAAGCCCAAACAATGAACAACGACAACAGATCTGAAGATTTTTTTTGGAGCAGCAGTGCTATTGAATTGGATCATGGTGCTATTCAGTCCCAGAGAAGCATGTCATCCATTTCCGTGTCAAACCATCCTTCTGATCCTCAAAGTAGTGATGGCATTCAGACTGATTCTCCAGAATTTGTCAATCATGGCAAGTCTTCTTCTGTGGAACCAAACAAGGCAACAATGGGTTGGAAATAA
Protein sequence

>Msa0432330

MLLVKPALQQSRGCLGCFSKPLVLLMGEADASKGLKTQAQTMNNDNRSEDFFWSSSAIELDHGAIQSQRSMSSISVSNHPSDPQSSDGIQTDSPEFVNHGKSSSVEPNKATMGWK*