AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414450 MtrunA17_Chr3g0107481 90.351 114 3 1 1 106 1 114 2.70e-72 210
Msa0414450 MtrunA17_Chr4g0013261 30.357 112 70 1 3 106 5 116 1.56e-15 66.6
Msa0414450 MtrunA17_Chr4g0013251 31.250 112 69 1 3 106 5 116 5.59e-15 65.5
Msa0414450 MtrunA17_Chr4g0013001 32.143 112 67 2 3 106 5 115 2.63e-13 60.8
Msa0414450 MtrunA17_Chr4g0012961 31.250 112 68 2 3 106 5 115 5.78e-13 60.1
Msa0414450 MtrunA17_Chr7g0244831 30.275 109 67 1 7 106 11 119 7.67e-13 60.1
Msa0414450 MtrunA17_Chr4g0012951 32.743 113 65 3 3 106 5 115 1.62e-12 58.9
Msa0414450 MtrunA17_Chr4g0012991 32.143 112 67 2 3 106 60 170 3.97e-12 59.3
Msa0414450 MtrunA17_Chr4g0012901 28.319 113 72 1 3 106 9 121 6.31e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACGATCTGGTGAATTTC+TGG 0.174462 3_2:+59983130 Msa0414450:three_prime_UTR
TAGTTGTTTGCTCACATGTT+AGG 0.273137 3_2:-59982196 None:intergenic
CTGGAAGATTATTCATTCTA+TGG 0.285035 3_2:+59983039 Msa0414450:three_prime_UTR
AGTTGCATGCCATTGCATTC+AGG 0.362920 3_2:+59982331 Msa0414450:CDS
TGCATACCCTATGGAATGTT+GGG 0.441117 3_2:+59982233 Msa0414450:CDS
AGTTGTTTGCTCACATGTTA+GGG 0.442335 3_2:-59982195 None:intergenic
TTCGAGTATAATCAATCAAA+GGG 0.448333 3_2:-59982370 None:intergenic
ATGCCATTGCATTCAGGATA+CGG 0.461881 3_2:+59982337 Msa0414450:CDS
ATCAATTATCAAATAGTGAA+AGG 0.476965 3_2:-59983188 None:intergenic
TGATCGTATATGTAACGATC+TGG 0.493714 3_2:+59983119 Msa0414450:three_prime_UTR
ATGCATACCCTATGGAATGT+TGG 0.496045 3_2:+59982232 Msa0414450:CDS
TTGTTACTAGTAGCTAGTGC+TGG 0.502388 3_2:+59983020 Msa0414450:three_prime_UTR
ATAAATGCTGCATACAAGAA+TGG 0.504762 3_2:+59982296 Msa0414450:CDS
CTAACTCCATGCATACCCTA+TGG 0.507572 3_2:+59982224 Msa0414450:CDS
TACTTATTGCAATCAGTGGA+GGG 0.513169 3_2:-59982443 None:intergenic
AGTGGAGGGGTAAACTTTGA+AGG 0.513675 3_2:-59982429 None:intergenic
ATTCGAGTATAATCAATCAA+AGG 0.524459 3_2:-59982371 None:intergenic
TCAATTATCAAATAGTGAAA+GGG 0.528436 3_2:-59983187 None:intergenic
AACAAATAGTCTTGCTCTCG+AGG 0.528711 3_2:-59983002 None:intergenic
AACATTCCATAGGGTATGCA+TGG 0.530105 3_2:-59982230 None:intergenic
GGCTTACTTATTGCAATCAG+TGG 0.540723 3_2:-59982447 None:intergenic
ACTTCCTCCCAACATTCCAT+AGG 0.556549 3_2:-59982240 None:intergenic
TATACTCGAATTAATCAACT+TGG 0.560190 3_2:+59982383 Msa0414450:CDS
TTACTTATTGCAATCAGTGG+AGG 0.569382 3_2:-59982444 None:intergenic
CAGGGTGAAGTAAGCAGCAA+AGG 0.583626 3_2:+59982967 Msa0414450:intron
AATCAACTTGGTGACCTATG+TGG 0.587360 3_2:+59982395 Msa0414450:CDS
CTTCCTCCCAACATTCCATA+GGG 0.593020 3_2:-59982239 None:intergenic
AGGGACATTTAGATCCACAT+AGG 0.605617 3_2:-59982409 None:intergenic
CAGCCGTATCCTGAATGCAA+TGG 0.607586 3_2:-59982340 None:intergenic
ACTTATTGCAATCAGTGGAG+GGG 0.616402 3_2:-59982442 None:intergenic
GTGGAGGGGTAAACTTTGAA+GGG 0.636043 3_2:-59982428 None:intergenic
ATACCCTATGGAATGTTGGG+AGG 0.689160 3_2:+59982236 Msa0414450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTACTTGGTTTGTTTTT+AGG + chr3_2:59982511-59982530 Msa0414450:intron 20.0%
!!! AATTTTAACTTCTTAACTTG+TGG + chr3_2:59982633-59982652 Msa0414450:intron 20.0%
!! ATGATTTATTTGATTCATCT+TGG + chr3_2:59982781-59982800 Msa0414450:intron 20.0%
!! TTACATCAAAATCCATAATT+AGG - chr3_2:59982942-59982961 None:intergenic 20.0%
!!! TATTTACTTGGTTTGTTTTT+AGG + chr3_2:59982511-59982530 Msa0414450:intron 20.0%
!!! AATTTTAACTTCTTAACTTG+TGG + chr3_2:59982633-59982652 Msa0414450:intron 20.0%
!! ATGATTTATTTGATTCATCT+TGG + chr3_2:59982781-59982800 Msa0414450:intron 20.0%
!! TTACATCAAAATCCATAATT+AGG - chr3_2:59982942-59982961 None:intergenic 20.0%
!!! GGTTGCATTATTTATTTTGA+TGG + chr3_2:59982136-59982155 Msa0414450:CDS 25.0%
! TTCGAGTATAATCAATCAAA+GGG - chr3_2:59982373-59982392 None:intergenic 25.0%
! ATTCGAGTATAATCAATCAA+AGG - chr3_2:59982374-59982393 None:intergenic 25.0%
! TATACTCGAATTAATCAACT+TGG + chr3_2:59982383-59982402 Msa0414450:CDS 25.0%
! AACCAAGTAAATAGAAAAGT+TGG - chr3_2:59982504-59982523 None:intergenic 25.0%
!! TTTTGTGCTAATTAACCTAA+AGG - chr3_2:59982682-59982701 None:intergenic 25.0%
! TACAAATCGAAGAGAAAATA+TGG - chr3_2:59982855-59982874 None:intergenic 25.0%
! TAGCAAACATATCCTAATTA+TGG + chr3_2:59982927-59982946 Msa0414450:intron 25.0%
!!! GGTTGCATTATTTATTTTGA+TGG + chr3_2:59982136-59982155 Msa0414450:CDS 25.0%
! TTCGAGTATAATCAATCAAA+GGG - chr3_2:59982373-59982392 None:intergenic 25.0%
! ATTCGAGTATAATCAATCAA+AGG - chr3_2:59982374-59982393 None:intergenic 25.0%
! TATACTCGAATTAATCAACT+TGG + chr3_2:59982383-59982402 Msa0414450:CDS 25.0%
! AACCAAGTAAATAGAAAAGT+TGG - chr3_2:59982504-59982523 None:intergenic 25.0%
!! TTTTGTGCTAATTAACCTAA+AGG - chr3_2:59982682-59982701 None:intergenic 25.0%
! TACAAATCGAAGAGAAAATA+TGG - chr3_2:59982855-59982874 None:intergenic 25.0%
! TAGCAAACATATCCTAATTA+TGG + chr3_2:59982927-59982946 Msa0414450:intron 25.0%
CATTTATAGAGTGAATACCT+TGG - chr3_2:59982283-59982302 None:intergenic 30.0%
ATAAATGCTGCATACAAGAA+TGG + chr3_2:59982296-59982315 Msa0414450:CDS 30.0%
! GTCCAACTTTTCTATTTACT+TGG + chr3_2:59982499-59982518 Msa0414450:intron 30.0%
!!! TTGGTTTGTTTTTAGGTCAA+GGG + chr3_2:59982518-59982537 Msa0414450:intron 30.0%
! TCATGCTAACTTTTGTCTTT+GGG - chr3_2:59982659-59982678 None:intergenic 30.0%
! TCGATTTGTAAAGCTTATAG+AGG + chr3_2:59982865-59982884 Msa0414450:intron 30.0%
CTGGAAGATTATTCATTCTA+TGG + chr3_2:59983039-59983058 Msa0414450:three_prime_UTR 30.0%
CATTTATAGAGTGAATACCT+TGG - chr3_2:59982283-59982302 None:intergenic 30.0%
ATAAATGCTGCATACAAGAA+TGG + chr3_2:59982296-59982315 Msa0414450:CDS 30.0%
! GTCCAACTTTTCTATTTACT+TGG + chr3_2:59982499-59982518 Msa0414450:intron 30.0%
!!! TTGGTTTGTTTTTAGGTCAA+GGG + chr3_2:59982518-59982537 Msa0414450:intron 30.0%
! TCATGCTAACTTTTGTCTTT+GGG - chr3_2:59982659-59982678 None:intergenic 30.0%
! TCGATTTGTAAAGCTTATAG+AGG + chr3_2:59982865-59982884 Msa0414450:intron 30.0%
CTGGAAGATTATTCATTCTA+TGG + chr3_2:59983039-59983058 Msa0414450:three_prime_UTR 30.0%
AGTTGTTTGCTCACATGTTA+GGG - chr3_2:59982198-59982217 None:intergenic 35.0%
TAGTTGTTTGCTCACATGTT+AGG - chr3_2:59982199-59982218 None:intergenic 35.0%
TACTTATTGCAATCAGTGGA+GGG - chr3_2:59982446-59982465 None:intergenic 35.0%
TTACTTATTGCAATCAGTGG+AGG - chr3_2:59982447-59982466 None:intergenic 35.0%
ACTAGTGTTACAAAGAGAGA+AGG - chr3_2:59982471-59982490 None:intergenic 35.0%
!!! CTTGGTTTGTTTTTAGGTCA+AGG + chr3_2:59982517-59982536 Msa0414450:intron 35.0%
ATTTCTTATCTTGTGCCCTA+GGG - chr3_2:59982559-59982578 None:intergenic 35.0%
TATTTCTTATCTTGTGCCCT+AGG - chr3_2:59982560-59982579 None:intergenic 35.0%
! GTCATGCTAACTTTTGTCTT+TGG - chr3_2:59982660-59982679 None:intergenic 35.0%
CAAAAGTTAGCATGACCTTT+AGG + chr3_2:59982664-59982683 Msa0414450:intron 35.0%
TGCAACTGTTTAGACCATAA+TGG - chr3_2:59982728-59982747 None:intergenic 35.0%
!! GATTTTGATGTAATTGCGCA+GGG + chr3_2:59982949-59982968 Msa0414450:intron 35.0%
TGATCGTATATGTAACGATC+TGG + chr3_2:59983119-59983138 Msa0414450:three_prime_UTR 35.0%
AGTTGTTTGCTCACATGTTA+GGG - chr3_2:59982198-59982217 None:intergenic 35.0%
TAGTTGTTTGCTCACATGTT+AGG - chr3_2:59982199-59982218 None:intergenic 35.0%
TACTTATTGCAATCAGTGGA+GGG - chr3_2:59982446-59982465 None:intergenic 35.0%
TTACTTATTGCAATCAGTGG+AGG - chr3_2:59982447-59982466 None:intergenic 35.0%
ACTAGTGTTACAAAGAGAGA+AGG - chr3_2:59982471-59982490 None:intergenic 35.0%
!!! CTTGGTTTGTTTTTAGGTCA+AGG + chr3_2:59982517-59982536 Msa0414450:intron 35.0%
ATTTCTTATCTTGTGCCCTA+GGG - chr3_2:59982559-59982578 None:intergenic 35.0%
TATTTCTTATCTTGTGCCCT+AGG - chr3_2:59982560-59982579 None:intergenic 35.0%
! GTCATGCTAACTTTTGTCTT+TGG - chr3_2:59982660-59982679 None:intergenic 35.0%
CAAAAGTTAGCATGACCTTT+AGG + chr3_2:59982664-59982683 Msa0414450:intron 35.0%
TGCAACTGTTTAGACCATAA+TGG - chr3_2:59982728-59982747 None:intergenic 35.0%
!! GATTTTGATGTAATTGCGCA+GGG + chr3_2:59982949-59982968 Msa0414450:intron 35.0%
TGATCGTATATGTAACGATC+TGG + chr3_2:59983119-59983138 Msa0414450:three_prime_UTR 35.0%
AACATTCCATAGGGTATGCA+TGG - chr3_2:59982233-59982252 None:intergenic 40.0%
ATGCATACCCTATGGAATGT+TGG + chr3_2:59982232-59982251 Msa0414450:CDS 40.0%
TGCATACCCTATGGAATGTT+GGG + chr3_2:59982233-59982252 Msa0414450:CDS 40.0%
TGAATACCTTGGCAACACAA+GGG - chr3_2:59982272-59982291 None:intergenic 40.0%
ATGCCATTGCATTCAGGATA+CGG + chr3_2:59982337-59982356 Msa0414450:CDS 40.0%
! AATCAACTTGGTGACCTATG+TGG + chr3_2:59982395-59982414 Msa0414450:CDS 40.0%
AGGGACATTTAGATCCACAT+AGG - chr3_2:59982412-59982431 None:intergenic 40.0%
ACTTATTGCAATCAGTGGAG+GGG - chr3_2:59982445-59982464 None:intergenic 40.0%
GGCTTACTTATTGCAATCAG+TGG - chr3_2:59982450-59982469 None:intergenic 40.0%
AGATCTTTACCGCACCATTA+TGG + chr3_2:59982711-59982730 Msa0414450:intron 40.0%
CTGTTTAGACCATAATGGTG+CGG - chr3_2:59982723-59982742 None:intergenic 40.0%
ATGGTCTAAACAGTTGCATG+TGG + chr3_2:59982730-59982749 Msa0414450:intron 40.0%
AACAGTTGCATGTGGATACT+CGG + chr3_2:59982738-59982757 Msa0414450:intron 40.0%
!! GGATTTTGATGTAATTGCGC+AGG + chr3_2:59982948-59982967 Msa0414450:intron 40.0%
! AACAAATAGTCTTGCTCTCG+AGG - chr3_2:59983005-59983024 None:intergenic 40.0%
TTGTTACTAGTAGCTAGTGC+TGG + chr3_2:59983020-59983039 Msa0414450:three_prime_UTR 40.0%
GTAACGATCTGGTGAATTTC+TGG + chr3_2:59983130-59983149 Msa0414450:three_prime_UTR 40.0%
AACATTCCATAGGGTATGCA+TGG - chr3_2:59982233-59982252 None:intergenic 40.0%
ATGCATACCCTATGGAATGT+TGG + chr3_2:59982232-59982251 Msa0414450:CDS 40.0%
TGCATACCCTATGGAATGTT+GGG + chr3_2:59982233-59982252 Msa0414450:CDS 40.0%
TGAATACCTTGGCAACACAA+GGG - chr3_2:59982272-59982291 None:intergenic 40.0%
ATGCCATTGCATTCAGGATA+CGG + chr3_2:59982337-59982356 Msa0414450:CDS 40.0%
! AATCAACTTGGTGACCTATG+TGG + chr3_2:59982395-59982414 Msa0414450:CDS 40.0%
AGGGACATTTAGATCCACAT+AGG - chr3_2:59982412-59982431 None:intergenic 40.0%
ACTTATTGCAATCAGTGGAG+GGG - chr3_2:59982445-59982464 None:intergenic 40.0%
GGCTTACTTATTGCAATCAG+TGG - chr3_2:59982450-59982469 None:intergenic 40.0%
AGATCTTTACCGCACCATTA+TGG + chr3_2:59982711-59982730 Msa0414450:intron 40.0%
CTGTTTAGACCATAATGGTG+CGG - chr3_2:59982723-59982742 None:intergenic 40.0%
ATGGTCTAAACAGTTGCATG+TGG + chr3_2:59982730-59982749 Msa0414450:intron 40.0%
AACAGTTGCATGTGGATACT+CGG + chr3_2:59982738-59982757 Msa0414450:intron 40.0%
!! GGATTTTGATGTAATTGCGC+AGG + chr3_2:59982948-59982967 Msa0414450:intron 40.0%
! AACAAATAGTCTTGCTCTCG+AGG - chr3_2:59983005-59983024 None:intergenic 40.0%
TTGTTACTAGTAGCTAGTGC+TGG + chr3_2:59983020-59983039 Msa0414450:three_prime_UTR 40.0%
GTAACGATCTGGTGAATTTC+TGG + chr3_2:59983130-59983149 Msa0414450:three_prime_UTR 40.0%
CTAACTCCATGCATACCCTA+TGG + chr3_2:59982224-59982243 Msa0414450:CDS 45.0%
ATACCCTATGGAATGTTGGG+AGG + chr3_2:59982236-59982255 Msa0414450:CDS 45.0%
CTTCCTCCCAACATTCCATA+GGG - chr3_2:59982242-59982261 None:intergenic 45.0%
ACTTCCTCCCAACATTCCAT+AGG - chr3_2:59982243-59982262 None:intergenic 45.0%
GTGAATACCTTGGCAACACA+AGG - chr3_2:59982273-59982292 None:intergenic 45.0%
AGTTGCATGCCATTGCATTC+AGG + chr3_2:59982331-59982350 Msa0414450:CDS 45.0%
GTGGAGGGGTAAACTTTGAA+GGG - chr3_2:59982431-59982450 None:intergenic 45.0%
AGTGGAGGGGTAAACTTTGA+AGG - chr3_2:59982432-59982451 None:intergenic 45.0%
AAATGCTACCTTGTGCCCTA+GGG + chr3_2:59982541-59982560 Msa0414450:intron 45.0%
CTAACTCCATGCATACCCTA+TGG + chr3_2:59982224-59982243 Msa0414450:CDS 45.0%
ATACCCTATGGAATGTTGGG+AGG + chr3_2:59982236-59982255 Msa0414450:CDS 45.0%
CTTCCTCCCAACATTCCATA+GGG - chr3_2:59982242-59982261 None:intergenic 45.0%
ACTTCCTCCCAACATTCCAT+AGG - chr3_2:59982243-59982262 None:intergenic 45.0%
GTGAATACCTTGGCAACACA+AGG - chr3_2:59982273-59982292 None:intergenic 45.0%
AGTTGCATGCCATTGCATTC+AGG + chr3_2:59982331-59982350 Msa0414450:CDS 45.0%
GTGGAGGGGTAAACTTTGAA+GGG - chr3_2:59982431-59982450 None:intergenic 45.0%
AGTGGAGGGGTAAACTTTGA+AGG - chr3_2:59982432-59982451 None:intergenic 45.0%
AAATGCTACCTTGTGCCCTA+GGG + chr3_2:59982541-59982560 Msa0414450:intron 45.0%
!!! TTTATTTTATTTTTTCTATT+TGG - chr3_2:59983093-59983112 None:intergenic 5.0%
!!! TTTATTTTATTTTTTCTATT+TGG - chr3_2:59983093-59983112 None:intergenic 5.0%
GTATCACCCTTGTGTTGCCA+AGG + chr3_2:59982263-59982282 Msa0414450:intron 50.0%
CAGCCGTATCCTGAATGCAA+TGG - chr3_2:59982343-59982362 None:intergenic 50.0%
GAAATGCTACCTTGTGCCCT+AGG + chr3_2:59982540-59982559 Msa0414450:intron 50.0%
CAGGGTGAAGTAAGCAGCAA+AGG + chr3_2:59982967-59982986 Msa0414450:intron 50.0%
GTATCACCCTTGTGTTGCCA+AGG + chr3_2:59982263-59982282 Msa0414450:intron 50.0%
CAGCCGTATCCTGAATGCAA+TGG - chr3_2:59982343-59982362 None:intergenic 50.0%
GAAATGCTACCTTGTGCCCT+AGG + chr3_2:59982540-59982559 Msa0414450:intron 50.0%
CAGGGTGAAGTAAGCAGCAA+AGG + chr3_2:59982967-59982986 Msa0414450:intron 50.0%
ATCTTGTGCCCTAGGGCACA+AGG - chr3_2:59982552-59982571 None:intergenic 55.0%
ATCTTGTGCCCTAGGGCACA+AGG - chr3_2:59982552-59982571 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59982128 59983201 59982128 ID=Msa0414450;Name=Msa0414450
chr3_2 mRNA 59982128 59983201 59982128 ID=Msa0414450-mRNA-1;Parent=Msa0414450;Name=Msa0414450-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.5|0.33|1|0|0|3|222|106
chr3_2 exon 59982128 59982260 59982128 ID=Msa0414450-mRNA-1:exon:12465;Parent=Msa0414450-mRNA-1
chr3_2 exon 59982285 59982462 59982285 ID=Msa0414450-mRNA-1:exon:12466;Parent=Msa0414450-mRNA-1
chr3_2 exon 59982970 59983201 59982970 ID=Msa0414450-mRNA-1:exon:12467;Parent=Msa0414450-mRNA-1
chr3_2 CDS 59982128 59982260 59982128 ID=Msa0414450-mRNA-1:cds;Parent=Msa0414450-mRNA-1
chr3_2 CDS 59982285 59982462 59982285 ID=Msa0414450-mRNA-1:cds;Parent=Msa0414450-mRNA-1
chr3_2 CDS 59982970 59982979 59982970 ID=Msa0414450-mRNA-1:cds;Parent=Msa0414450-mRNA-1
chr3_2 three_prime_UTR 59982980 59983201 59982980 ID=Msa0414450-mRNA-1:three_prime_utr;Parent=Msa0414450-mRNA-1
Gene Sequence

>Msa0414450

ATGGGTAAGGTTGCATTATTTATTTTGATGGCTTGCATGATGATAGCAAGTTCTCATGCTAAAGCAACCCTAACATGTGAGCAAACAACTATATATCTAACTCCATGCATACCCTATGGAATGTTGGGAGGAAGTATTCACTCTATAAATGCTGCATACAAGAATGGTGATGATAGAAGAGTTGCATGCCATTGCATTCAGGATACGGCTGCTAAAATCCCTTTGATTGATTATACTCGAATTAATCAACTTGGTGACCTATGTGGATCTAAATGTCCCTTCAAAGTTTACCCCTCCACTGATTGCAATAAGGTGAAGTAA

Protein sequence

>Msa0414450

MGKVALFILMACMMIASSHAKATLTCEQTTIYLTPCIPYGMLGGSIHSINAAYKNGDDRRVACHCIQDTAAKIPLIDYTRINQLGDLCGSKCPFKVYPSTDCNKVK*