AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459270 MtrunA17_Chr3g0107481 90.179 112 3 1 1 104 1 112 3.56e-70 206
Msa0459270 MtrunA17_Chr4g0013261 30.909 110 68 1 3 104 5 114 1.33e-14 65.5
Msa0459270 MtrunA17_Chr4g0013251 32.727 110 66 1 3 104 5 114 3.77e-14 64.3
Msa0459270 MtrunA17_Chr4g0013001 34.259 108 62 2 3 102 5 111 1.01e-12 60.5
Msa0459270 MtrunA17_Chr4g0012961 33.333 108 63 2 3 102 5 111 1.94e-12 59.7
Msa0459270 MtrunA17_Chr4g0012991 34.259 108 62 2 3 102 60 166 4.38e-12 60.1
Msa0459270 MtrunA17_Chr4g0012951 33.945 109 61 3 3 102 5 111 1.20e-11 57.8
Msa0459270 MtrunA17_Chr7g0244831 29.907 107 66 1 7 104 11 117 2.40e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTTGTTTGCTCACATGTT+AGG 0.273137 3_3:-55761972 None:intergenic
AGTGCTGGAAGATTATTCTA+TGG 0.437007 3_3:+55762851 Msa0459270:CDS
TGCATACCCTATGGAATGTT+GGG 0.441117 3_3:+55762009 Msa0459270:CDS
AGTTGTTTGCTCACATGTTA+GGG 0.442335 3_3:-55761971 None:intergenic
TTCGAGTATAATCAATCAAA+GGG 0.448333 3_3:-55762146 None:intergenic
TTGTTACCAGTAGCTAGTGC+TGG 0.483688 3_3:+55762836 Msa0459270:CDS
ATGCATACCCTATGGAATGT+TGG 0.496045 3_3:+55762008 Msa0459270:CDS
ATGCCATTGCATTCAAGATA+CGG 0.502113 3_3:+55762113 Msa0459270:CDS
ATAAATGCTGCATATAAGAA+TGG 0.504762 3_3:+55762072 Msa0459270:CDS
CTAACTCCATGCATACCCTA+TGG 0.507572 3_3:+55762000 Msa0459270:CDS
TACTTATTGCAATCAGTGGA+GGG 0.513169 3_3:-55762219 None:intergenic
AGTGGAGGGGTAAACTTTGA+AGG 0.513675 3_3:-55762205 None:intergenic
ATTCGAGTATAATCAATCAA+AGG 0.524459 3_3:-55762147 None:intergenic
AATCTTCCAGCACTAGCTAC+TGG 0.526580 3_3:-55762842 None:intergenic
AACAAATAGTCTTGCTCTCG+AGG 0.528711 3_3:-55762818 None:intergenic
AACATTCCATAGGGTATGCA+TGG 0.530105 3_3:-55762006 None:intergenic
GGCTTACTTATTGCAATCAG+TGG 0.540723 3_3:-55762223 None:intergenic
CAGCCGTATCTTGAATGCAA+TGG 0.548360 3_3:-55762116 None:intergenic
TATACTCGAATTAATCAACT+TGG 0.560190 3_3:+55762159 Msa0459270:CDS
TTACTTATTGCAATCAGTGG+AGG 0.569382 3_3:-55762220 None:intergenic
ACTCCCTCCCAACATTCCAT+AGG 0.570029 3_3:-55762016 None:intergenic
CTCCCTCCCAACATTCCATA+GGG 0.578201 3_3:-55762015 None:intergenic
CAGGGTGAAGTAAGCAGCAA+AGG 0.583626 3_3:+55762783 Msa0459270:intron
TACCCTATGGAATGTTGGGA+GGG 0.587169 3_3:+55762013 Msa0459270:CDS
AATCAACTTGGTGACCTATG+TGG 0.587360 3_3:+55762171 Msa0459270:CDS
AGGGACATTTAGATCCACAT+AGG 0.605617 3_3:-55762185 None:intergenic
ACTTATTGCAATCAGTGGAG+GGG 0.616402 3_3:-55762218 None:intergenic
GTGGAGGGGTAAACTTTGAA+GGG 0.636043 3_3:-55762204 None:intergenic
ATACCCTATGGAATGTTGGG+AGG 0.662835 3_3:+55762012 Msa0459270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTACTTGGTTTGTTTTT+AGG + chr3_3:55762285-55762304 Msa0459270:intron 20.0%
!! ATGATTTATTTGATTCATGT+TGG + chr3_3:55762596-55762615 Msa0459270:intron 20.0%
!! CTATAATTGAAGAGAAAATA+TGG - chr3_3:55762670-55762689 None:intergenic 20.0%
!! TTACATCAAAATCCATAATT+AGG - chr3_3:55762758-55762777 None:intergenic 20.0%
!!! TATTTACTTGGTTTGTTTTT+AGG + chr3_3:55762285-55762304 Msa0459270:intron 20.0%
!! ATGATTTATTTGATTCATGT+TGG + chr3_3:55762596-55762615 Msa0459270:intron 20.0%
!! CTATAATTGAAGAGAAAATA+TGG - chr3_3:55762670-55762689 None:intergenic 20.0%
!! TTACATCAAAATCCATAATT+AGG - chr3_3:55762758-55762777 None:intergenic 20.0%
! ATAAATGCTGCATATAAGAA+TGG + chr3_3:55762072-55762091 Msa0459270:CDS 25.0%
! TTCGAGTATAATCAATCAAA+GGG - chr3_3:55762149-55762168 None:intergenic 25.0%
! ATTCGAGTATAATCAATCAA+AGG - chr3_3:55762150-55762169 None:intergenic 25.0%
! TATACTCGAATTAATCAACT+TGG + chr3_3:55762159-55762178 Msa0459270:CDS 25.0%
! ACATGAATATACTAGATACA+AGG - chr3_3:55762255-55762274 None:intergenic 25.0%
! AACCAAGTAAATAGAAAAGT+TGG - chr3_3:55762278-55762297 None:intergenic 25.0%
!!! TTGGTTTGTTTTTAGGTTAA+GGG + chr3_3:55762292-55762311 Msa0459270:intron 25.0%
!! TTTAGACTAATCTTTGAGTT+AGG - chr3_3:55762535-55762554 None:intergenic 25.0%
! TACCAAACATATCCTAATTA+TGG + chr3_3:55762743-55762762 Msa0459270:intron 25.0%
! ATCCATAATTAGGATATGTT+TGG - chr3_3:55762748-55762767 None:intergenic 25.0%
! ATAAATGCTGCATATAAGAA+TGG + chr3_3:55762072-55762091 Msa0459270:CDS 25.0%
! TTCGAGTATAATCAATCAAA+GGG - chr3_3:55762149-55762168 None:intergenic 25.0%
! ATTCGAGTATAATCAATCAA+AGG - chr3_3:55762150-55762169 None:intergenic 25.0%
! TATACTCGAATTAATCAACT+TGG + chr3_3:55762159-55762178 Msa0459270:CDS 25.0%
! ACATGAATATACTAGATACA+AGG - chr3_3:55762255-55762274 None:intergenic 25.0%
! AACCAAGTAAATAGAAAAGT+TGG - chr3_3:55762278-55762297 None:intergenic 25.0%
!!! TTGGTTTGTTTTTAGGTTAA+GGG + chr3_3:55762292-55762311 Msa0459270:intron 25.0%
!! TTTAGACTAATCTTTGAGTT+AGG - chr3_3:55762535-55762554 None:intergenic 25.0%
! TACCAAACATATCCTAATTA+TGG + chr3_3:55762743-55762762 Msa0459270:intron 25.0%
! ATCCATAATTAGGATATGTT+TGG - chr3_3:55762748-55762767 None:intergenic 25.0%
!!! GGTTGCATTATTTGTTTTGA+TGG + chr3_3:55761912-55761931 Msa0459270:CDS 30.0%
CATTTATAGAGTGAATACCT+TGG - chr3_3:55762059-55762078 None:intergenic 30.0%
! GTCCAACTTTTCTATTTACT+TGG + chr3_3:55762273-55762292 Msa0459270:intron 30.0%
!!! CTTGGTTTGTTTTTAGGTTA+AGG + chr3_3:55762291-55762310 Msa0459270:intron 30.0%
! TAATTGGCACAAAACTTTTG+AGG + chr3_3:55762480-55762499 Msa0459270:intron 30.0%
TACAAAACTCATTGAATGAG+TGG - chr3_3:55762710-55762729 None:intergenic 30.0%
!!! GGTTGCATTATTTGTTTTGA+TGG + chr3_3:55761912-55761931 Msa0459270:CDS 30.0%
CATTTATAGAGTGAATACCT+TGG - chr3_3:55762059-55762078 None:intergenic 30.0%
! GTCCAACTTTTCTATTTACT+TGG + chr3_3:55762273-55762292 Msa0459270:intron 30.0%
!!! CTTGGTTTGTTTTTAGGTTA+AGG + chr3_3:55762291-55762310 Msa0459270:intron 30.0%
! TAATTGGCACAAAACTTTTG+AGG + chr3_3:55762480-55762499 Msa0459270:intron 30.0%
TACAAAACTCATTGAATGAG+TGG - chr3_3:55762710-55762729 None:intergenic 30.0%
AGTTGTTTGCTCACATGTTA+GGG - chr3_3:55761974-55761993 None:intergenic 35.0%
TAGTTGTTTGCTCACATGTT+AGG - chr3_3:55761975-55761994 None:intergenic 35.0%
ATGCCATTGCATTCAAGATA+CGG + chr3_3:55762113-55762132 Msa0459270:CDS 35.0%
TACTTATTGCAATCAGTGGA+GGG - chr3_3:55762222-55762241 None:intergenic 35.0%
TTACTTATTGCAATCAGTGG+AGG - chr3_3:55762223-55762242 None:intergenic 35.0%
ATACTAGATACAAGGAGAGA+AGG - chr3_3:55762247-55762266 None:intergenic 35.0%
TTTCTTATCTTGTGCCCTTA+GGG - chr3_3:55762333-55762352 None:intergenic 35.0%
ATTTCTTATCTTGTGCCCTT+AGG - chr3_3:55762334-55762353 None:intergenic 35.0%
TTAGTCTAAACAGTTGCATG+TGG + chr3_3:55762545-55762564 Msa0459270:intron 35.0%
!! GATTTTGATGTAATTGCGCA+GGG + chr3_3:55762765-55762784 Msa0459270:intron 35.0%
! AGTGCTGGAAGATTATTCTA+TGG + chr3_3:55762851-55762870 Msa0459270:CDS 35.0%
AGTTGTTTGCTCACATGTTA+GGG - chr3_3:55761974-55761993 None:intergenic 35.0%
TAGTTGTTTGCTCACATGTT+AGG - chr3_3:55761975-55761994 None:intergenic 35.0%
ATGCCATTGCATTCAAGATA+CGG + chr3_3:55762113-55762132 Msa0459270:CDS 35.0%
TACTTATTGCAATCAGTGGA+GGG - chr3_3:55762222-55762241 None:intergenic 35.0%
TTACTTATTGCAATCAGTGG+AGG - chr3_3:55762223-55762242 None:intergenic 35.0%
ATACTAGATACAAGGAGAGA+AGG - chr3_3:55762247-55762266 None:intergenic 35.0%
TTTCTTATCTTGTGCCCTTA+GGG - chr3_3:55762333-55762352 None:intergenic 35.0%
ATTTCTTATCTTGTGCCCTT+AGG - chr3_3:55762334-55762353 None:intergenic 35.0%
TTAGTCTAAACAGTTGCATG+TGG + chr3_3:55762545-55762564 Msa0459270:intron 35.0%
!! GATTTTGATGTAATTGCGCA+GGG + chr3_3:55762765-55762784 Msa0459270:intron 35.0%
! AGTGCTGGAAGATTATTCTA+TGG + chr3_3:55762851-55762870 Msa0459270:CDS 35.0%
AACATTCCATAGGGTATGCA+TGG - chr3_3:55762009-55762028 None:intergenic 40.0%
ATGCATACCCTATGGAATGT+TGG + chr3_3:55762008-55762027 Msa0459270:CDS 40.0%
TGCATACCCTATGGAATGTT+GGG + chr3_3:55762009-55762028 Msa0459270:CDS 40.0%
TGAATACCTTGGCAACACAA+GGG - chr3_3:55762048-55762067 None:intergenic 40.0%
! AATCAACTTGGTGACCTATG+TGG + chr3_3:55762171-55762190 Msa0459270:CDS 40.0%
AGGGACATTTAGATCCACAT+AGG - chr3_3:55762188-55762207 None:intergenic 40.0%
ACTTATTGCAATCAGTGGAG+GGG - chr3_3:55762221-55762240 None:intergenic 40.0%
GGCTTACTTATTGCAATCAG+TGG - chr3_3:55762226-55762245 None:intergenic 40.0%
ACTTCTTAACTCGTGCCTTA+AGG + chr3_3:55762432-55762451 Msa0459270:intron 40.0%
! CATGCTAACTTGTGTCCTTA+AGG - chr3_3:55762450-55762469 None:intergenic 40.0%
AGCATGACGCTTAGGTTAAT+TGG + chr3_3:55762464-55762483 Msa0459270:intron 40.0%
AACAGTTGCATGTGGATACT+CGG + chr3_3:55762553-55762572 Msa0459270:intron 40.0%
!! GGATTTTGATGTAATTGCGC+AGG + chr3_3:55762764-55762783 Msa0459270:intron 40.0%
! AACAAATAGTCTTGCTCTCG+AGG - chr3_3:55762821-55762840 None:intergenic 40.0%
AACATTCCATAGGGTATGCA+TGG - chr3_3:55762009-55762028 None:intergenic 40.0%
ATGCATACCCTATGGAATGT+TGG + chr3_3:55762008-55762027 Msa0459270:CDS 40.0%
TGCATACCCTATGGAATGTT+GGG + chr3_3:55762009-55762028 Msa0459270:CDS 40.0%
TGAATACCTTGGCAACACAA+GGG - chr3_3:55762048-55762067 None:intergenic 40.0%
! AATCAACTTGGTGACCTATG+TGG + chr3_3:55762171-55762190 Msa0459270:CDS 40.0%
AGGGACATTTAGATCCACAT+AGG - chr3_3:55762188-55762207 None:intergenic 40.0%
ACTTATTGCAATCAGTGGAG+GGG - chr3_3:55762221-55762240 None:intergenic 40.0%
GGCTTACTTATTGCAATCAG+TGG - chr3_3:55762226-55762245 None:intergenic 40.0%
ACTTCTTAACTCGTGCCTTA+AGG + chr3_3:55762432-55762451 Msa0459270:intron 40.0%
! CATGCTAACTTGTGTCCTTA+AGG - chr3_3:55762450-55762469 None:intergenic 40.0%
AGCATGACGCTTAGGTTAAT+TGG + chr3_3:55762464-55762483 Msa0459270:intron 40.0%
AACAGTTGCATGTGGATACT+CGG + chr3_3:55762553-55762572 Msa0459270:intron 40.0%
!! GGATTTTGATGTAATTGCGC+AGG + chr3_3:55762764-55762783 Msa0459270:intron 40.0%
! AACAAATAGTCTTGCTCTCG+AGG - chr3_3:55762821-55762840 None:intergenic 40.0%
CTAACTCCATGCATACCCTA+TGG + chr3_3:55762000-55762019 Msa0459270:CDS 45.0%
ATACCCTATGGAATGTTGGG+AGG + chr3_3:55762012-55762031 Msa0459270:CDS 45.0%
TACCCTATGGAATGTTGGGA+GGG + chr3_3:55762013-55762032 Msa0459270:CDS 45.0%
GTGAATACCTTGGCAACACA+AGG - chr3_3:55762049-55762068 None:intergenic 45.0%
CAGCCGTATCTTGAATGCAA+TGG - chr3_3:55762119-55762138 None:intergenic 45.0%
GTGGAGGGGTAAACTTTGAA+GGG - chr3_3:55762207-55762226 None:intergenic 45.0%
AGTGGAGGGGTAAACTTTGA+AGG - chr3_3:55762208-55762227 None:intergenic 45.0%
AAATGCTACCTTGTGCCCTA+AGG + chr3_3:55762315-55762334 Msa0459270:intron 45.0%
AATGCTACCTTGTGCCCTAA+GGG + chr3_3:55762316-55762335 Msa0459270:intron 45.0%
CACAAGTTAGCATGACGCTT+AGG + chr3_3:55762456-55762475 Msa0459270:intron 45.0%
GAGTTAGGATATGACCACGA+TGG - chr3_3:55762520-55762539 None:intergenic 45.0%
TTGTTACCAGTAGCTAGTGC+TGG + chr3_3:55762836-55762855 Msa0459270:CDS 45.0%
AATCTTCCAGCACTAGCTAC+TGG - chr3_3:55762845-55762864 None:intergenic 45.0%
CTAACTCCATGCATACCCTA+TGG + chr3_3:55762000-55762019 Msa0459270:CDS 45.0%
ATACCCTATGGAATGTTGGG+AGG + chr3_3:55762012-55762031 Msa0459270:CDS 45.0%
TACCCTATGGAATGTTGGGA+GGG + chr3_3:55762013-55762032 Msa0459270:CDS 45.0%
GTGAATACCTTGGCAACACA+AGG - chr3_3:55762049-55762068 None:intergenic 45.0%
CAGCCGTATCTTGAATGCAA+TGG - chr3_3:55762119-55762138 None:intergenic 45.0%
GTGGAGGGGTAAACTTTGAA+GGG - chr3_3:55762207-55762226 None:intergenic 45.0%
AGTGGAGGGGTAAACTTTGA+AGG - chr3_3:55762208-55762227 None:intergenic 45.0%
AAATGCTACCTTGTGCCCTA+AGG + chr3_3:55762315-55762334 Msa0459270:intron 45.0%
AATGCTACCTTGTGCCCTAA+GGG + chr3_3:55762316-55762335 Msa0459270:intron 45.0%
CACAAGTTAGCATGACGCTT+AGG + chr3_3:55762456-55762475 Msa0459270:intron 45.0%
GAGTTAGGATATGACCACGA+TGG - chr3_3:55762520-55762539 None:intergenic 45.0%
TTGTTACCAGTAGCTAGTGC+TGG + chr3_3:55762836-55762855 Msa0459270:CDS 45.0%
AATCTTCCAGCACTAGCTAC+TGG - chr3_3:55762845-55762864 None:intergenic 45.0%
CTCCCTCCCAACATTCCATA+GGG - chr3_3:55762018-55762037 None:intergenic 50.0%
ACTCCCTCCCAACATTCCAT+AGG - chr3_3:55762019-55762038 None:intergenic 50.0%
GTATCACCCTTGTGTTGCCA+AGG + chr3_3:55762039-55762058 Msa0459270:intron 50.0%
AGATCTTTACCGCACCATCG+TGG + chr3_3:55762503-55762522 Msa0459270:intron 50.0%
AGGATATGACCACGATGGTG+CGG - chr3_3:55762515-55762534 None:intergenic 50.0%
CAGGGTGAAGTAAGCAGCAA+AGG + chr3_3:55762783-55762802 Msa0459270:intron 50.0%
CTCCCTCCCAACATTCCATA+GGG - chr3_3:55762018-55762037 None:intergenic 50.0%
ACTCCCTCCCAACATTCCAT+AGG - chr3_3:55762019-55762038 None:intergenic 50.0%
GTATCACCCTTGTGTTGCCA+AGG + chr3_3:55762039-55762058 Msa0459270:intron 50.0%
AGATCTTTACCGCACCATCG+TGG + chr3_3:55762503-55762522 Msa0459270:intron 50.0%
AGGATATGACCACGATGGTG+CGG - chr3_3:55762515-55762534 None:intergenic 50.0%
CAGGGTGAAGTAAGCAGCAA+AGG + chr3_3:55762783-55762802 Msa0459270:intron 50.0%
TCTTGTGCCCTTAGGGCACA+AGG - chr3_3:55762326-55762345 None:intergenic 55.0%
TCTTGTGCCCTTAGGGCACA+AGG - chr3_3:55762326-55762345 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 55761904 55762880 55761904 ID=Msa0459270;Name=Msa0459270
chr3_3 mRNA 55761904 55762880 55761904 ID=Msa0459270-mRNA-1;Parent=Msa0459270;Name=Msa0459270-mRNA-1;_AED=0.34;_eAED=0.49;_QI=0|0|0|1|0|0|3|0|132
chr3_3 exon 55761904 55762036 55761904 ID=Msa0459270-mRNA-1:exon:11072;Parent=Msa0459270-mRNA-1
chr3_3 exon 55762061 55762238 55762061 ID=Msa0459270-mRNA-1:exon:11073;Parent=Msa0459270-mRNA-1
chr3_3 exon 55762793 55762880 55762793 ID=Msa0459270-mRNA-1:exon:11074;Parent=Msa0459270-mRNA-1
chr3_3 CDS 55761904 55762036 55761904 ID=Msa0459270-mRNA-1:cds;Parent=Msa0459270-mRNA-1
chr3_3 CDS 55762061 55762238 55762061 ID=Msa0459270-mRNA-1:cds;Parent=Msa0459270-mRNA-1
chr3_3 CDS 55762793 55762880 55762793 ID=Msa0459270-mRNA-1:cds;Parent=Msa0459270-mRNA-1
Gene Sequence

>Msa0459270

ATGGGTAAGGTTGCATTATTTGTTTTGATGGCTTGCATGATGATAGCAAGTTCTCATGCAAAAGCAACCCTAACATGTGAGCAAACAACTATATATCTAACTCCATGCATACCCTATGGAATGTTGGGAGGGAGTATTCACTCTATAAATGCTGCATATAAGAATGGTGATGATAGAAGAGTTGCATGCCATTGCATTCAAGATACGGCTGCAAAAATCCCTTTGATTGATTATACTCGAATTAATCAACTTGGTGACCTATGTGGATCTAAATGTCCCTTCAAAGTTTACCCCTCCACTGATTGCAATAATAAGCAGCAAAGGTTGCAACATATACCTCGAGAGCAAGACTATTTGTTACCAGTAGCTAGTGCTGGAAGATTATTCTATGGAGAATAA

Protein sequence

>Msa0459270

MGKVALFVLMACMMIASSHAKATLTCEQTTIYLTPCIPYGMLGGSIHSINAAYKNGDDRRVACHCIQDTAAKIPLIDYTRINQLGDLCGSKCPFKVYPSTDCNNKQQRLQHIPREQDYLLPVASAGRLFYGE*