AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0417960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0417960 MtrunA17_Chr3g0115551 98.718 156 2 0 1 156 1 156 1.69e-115 323
Msa0417960 MtrunA17_Chr3g0124361 37.791 172 73 7 1 138 1 172 4.24e-25 95.5
Msa0417960 MtrunA17_Chr2g0295651 45.588 68 35 1 65 132 41 106 1.37e-15 69.3
Msa0417960 MtrunA17_Chr8g0336591 45.312 64 35 0 81 144 29 92 3.41e-15 67.4
Msa0417960 MtrunA17_Chr5g0400051 59.184 49 19 1 86 134 81 128 6.34e-15 68.2
Msa0417960 MtrunA17_Chr4g0067541 58.140 43 18 0 90 132 62 104 1.92e-14 65.5
Msa0417960 MtrunA17_Chr2g0301141 59.574 47 18 1 89 135 185 230 2.21e-13 65.5
Msa0417960 MtrunA17_Chr4g0055191 53.061 49 23 0 86 134 69 117 7.26e-13 62.4
Msa0417960 MtrunA17_Chr4g0070221 44.828 58 31 1 90 147 178 234 4.00e-12 62.0
Msa0417960 MtrunA17_Chr8g0344991 52.273 44 21 0 90 133 39 82 1.97e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0417960 AT1G78020.1 52.817 142 50 6 1 134 1 133 1.81e-41 136
Msa0417960 AT1G22160.1 50.000 150 61 5 1 149 1 137 1.36e-40 134
Msa0417960 AT4G39795.1 38.298 141 64 2 1 138 1 121 6.92e-25 93.2
Msa0417960 AT5G65040.1 37.324 142 56 4 1 142 1 109 1.34e-21 84.3
Msa0417960 AT5G49120.1 50.704 71 27 2 76 142 58 124 2.14e-17 74.7
Msa0417960 AT4G17670.1 60.417 48 19 0 87 134 73 120 8.84e-16 70.5
Msa0417960 AT5G47060.2 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0417960 AT5G47060.1 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0417960 AT2G44670.1 53.191 47 22 0 88 134 14 60 1.91e-13 62.8
Msa0417960 AT1G19200.2 44.444 81 38 3 73 153 149 222 2.82e-13 65.1
Msa0417960 AT1G19200.1 44.444 81 38 3 73 153 136 209 3.24e-13 65.1
Msa0417960 AT1G74940.1 47.143 70 31 3 89 153 149 217 3.63e-13 64.7
Msa0417960 AT5G20700.1 50.000 46 22 1 90 135 182 226 1.14e-11 61.2
Msa0417960 AT1G53885.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0
Msa0417960 AT1G53903.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0

Find 55 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTCTTTGATTCTTAAT+AGG 0.201917 3_2:-65433985 Msa0417960:three_prime_UTR
CCAGGAGAAGTTGGTGGATA+TGG 0.255956 3_2:+65434799 None:intergenic
GAATTTCTTCTAAGGTTTCT+AGG 0.264307 3_2:+65434733 None:intergenic
AACAGTTGCTTCAAAGTTAC+TGG 0.297254 3_2:+65433962 None:intergenic
GCCATCAATTTATCCTTGTT+TGG 0.304676 3_2:+65434173 None:intergenic
TATCAGCTGAATTTCTTCTA+AGG 0.311688 3_2:+65434725 None:intergenic
AAGAAGAAGAATGATGAAAA+AGG 0.318738 3_2:+65435035 None:intergenic
AAGCAAGAGCGTAAGAAATC+AGG 0.327281 3_2:+65434691 None:intergenic
ATTAAAGATGGTGATGGGTT+TGG 0.327934 3_2:-65433817 Msa0417960:three_prime_UTR
AGAAGTAAGAAGATGATGTT+AGG 0.329332 3_2:-65434964 Msa0417960:exon
TTAAAGATGGTGATGGGTTT+GGG 0.347252 3_2:-65433816 Msa0417960:three_prime_UTR
GAAGAAAAGAGTGAAGCTTA+AGG 0.359309 3_2:+65435085 None:intergenic
GGGTTTGGGAAGTCTTTATA+TGG 0.364716 3_2:-65433802 None:intergenic
TGATCTGAACCGTTGATTCC+AGG 0.372333 3_2:+65434781 None:intergenic
CTTTCTCTGTAAACGCCGTT+TGG 0.383139 3_2:-65434671 Msa0417960:CDS
AAGAAAAGAGTGAAGCTTAA+GGG 0.390991 3_2:+65435086 None:intergenic
TGTAAACGCCGTTTGGTACC+TGG 0.425032 3_2:-65434664 Msa0417960:CDS
TCCAAACAAGGATAAATTGA+TGG 0.440186 3_2:-65434174 Msa0417960:three_prime_UTR
TTTCATATGCTACAAATTTG+TGG 0.444162 3_2:+65434199 None:intergenic
ATAATTTACCCAGGGACATA+TGG 0.444772 3_2:+65434134 None:intergenic
AAACAAGTTGTGATTGCAGC+TGG 0.446504 3_2:-65434236 Msa0417960:CDS
CCATATCCACCAACTTCTCC+TGG 0.454006 3_2:-65434799 Msa0417960:CDS
GAAGTAAGAAGATGATGTTA+GGG 0.462099 3_2:-65434963 Msa0417960:exon
CAGGTCCATGACGGTTGAAA+AGG 0.471195 3_2:+65434839 None:intergenic
ATTAATTAGCCATATGTCCC+TGG 0.478134 3_2:-65434143 Msa0417960:three_prime_UTR
AACCGTCATGGACCTGGTGT+TGG 0.498303 3_2:-65434832 Msa0417960:CDS
AGCAAGAGCGTAAGAAATCA+GGG 0.500012 3_2:+65434692 None:intergenic
AGAAAAGAGTGAAGCTTAAG+GGG 0.501406 3_2:+65435087 None:intergenic
GGACCTGGTGTTGGTCCGTA+CGG 0.509388 3_2:-65434823 Msa0417960:CDS
TCAACGGTTCAGATCAGAGC+AGG 0.509698 3_2:-65434774 Msa0417960:CDS
CCGTTGATTCCAGGAGAAGT+TGG 0.513302 3_2:+65434790 None:intergenic
TGTTACTGTGTTCAGATCGA+AGG 0.548881 3_2:+65434891 None:intergenic
TAAATAATAATAATTTACCC+AGG 0.552107 3_2:+65434125 None:intergenic
GTGGAAATTAAAGATGGTGA+TGG 0.555896 3_2:-65433823 Msa0417960:three_prime_UTR
ATTACAAAGCCAGAACCAAA+AGG 0.558823 3_2:+65434032 None:intergenic
TTGATTCCAGGAGAAGTTGG+TGG 0.560507 3_2:+65434793 None:intergenic
AAATAATAATAATTTACCCA+GGG 0.565763 3_2:+65434126 None:intergenic
CCTATTAAGAATCAAAGAAG+TGG 0.567121 3_2:+65433985 None:intergenic
TATGATGACTCAAAATCCAA+TGG 0.572170 3_2:+65433932 None:intergenic
TCAGAGCAGGGTTATGTCCA+TGG 0.575433 3_2:-65434761 Msa0417960:CDS
AACAAGTTGTGATTGCAGCT+GGG 0.591489 3_2:-65434235 Msa0417960:CDS
GATTGCAGCTGGGTCTAAAG+TGG 0.592297 3_2:-65434225 Msa0417960:CDS
TTAATTAGCCATATGTCCCT+GGG 0.593209 3_2:-65434142 Msa0417960:three_prime_UTR
GTTGGTGGATATGGACCGTA+CGG 0.599015 3_2:+65434808 None:intergenic
GGACCGTACGGACCAACACC+AGG 0.604586 3_2:+65434820 None:intergenic
CCAACTTCTCCTGGAATCAA+CGG 0.605664 3_2:-65434790 Msa0417960:CDS
TGTCACGACCAGGTACCAAA+CGG 0.608597 3_2:+65434656 None:intergenic
TGGAAATTAAAGATGGTGAT+GGG 0.610558 3_2:-65433822 Msa0417960:three_prime_UTR
AAGGTTTCTAGGTGATACCA+TGG 0.614252 3_2:+65434744 None:intergenic
AAAACAATAACCTAAAACTG+AGG 0.624254 3_2:+65435115 None:intergenic
GAAGAAAGATAAGTGTTCTG+TGG 0.638421 3_2:-65434267 Msa0417960:CDS
GACCAACACCAGGTCCATGA+CGG 0.641553 3_2:+65434830 None:intergenic
TACATGTAGATGTCACGACC+AGG 0.649454 3_2:+65434646 None:intergenic
GCATATGAAATATCCAAACA+AGG 0.656325 3_2:-65434186 Msa0417960:three_prime_UTR
CAACGGTTCAGATCAGAGCA+GGG 0.716813 3_2:-65434773 Msa0417960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATTATTAATTTTGTG+TGG - chr3_2:65434535-65434554 Msa0417960:intron 10.0%
!!! TTATTATTATTAATTTTGTG+TGG - chr3_2:65434535-65434554 Msa0417960:intron 10.0%
!! AAATAATAATAATTTACCCA+GGG + chr3_2:65434814-65434833 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG + chr3_2:65434815-65434834 None:intergenic 15.0%
!! AAATAATAATAATTTACCCA+GGG + chr3_2:65434814-65434833 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG + chr3_2:65434815-65434834 None:intergenic 15.0%
!!! GATTAATTGTATATTTTGTG+TGG - chr3_2:65435095-65435114 Msa0417960:five_prime_UTR 20.0%
!!! GATTAATTGTATATTTTGTG+TGG - chr3_2:65435095-65435114 Msa0417960:five_prime_UTR 20.0%
! AAAACAATAACCTAAAACTG+AGG + chr3_2:65433825-65433844 None:intergenic 25.0%
! AAGAAGAAGAATGATGAAAA+AGG + chr3_2:65433905-65433924 None:intergenic 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG - chr3_2:65434362-65434381 Msa0417960:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG - chr3_2:65434545-65434564 Msa0417960:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG - chr3_2:65434546-65434565 Msa0417960:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG - chr3_2:65434593-65434612 Msa0417960:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG + chr3_2:65434741-65434760 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG + chr3_2:65434872-65434891 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG + chr3_2:65434873-65434892 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG - chr3_2:65434872-65434891 Msa0417960:CDS 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG - chr3_2:65434916-65434935 Msa0417960:CDS 25.0%
!! TTTTGTGTGGAAATTAAAGA+TGG - chr3_2:65435108-65435127 Msa0417960:five_prime_UTR 25.0%
! AAAACAATAACCTAAAACTG+AGG + chr3_2:65433825-65433844 None:intergenic 25.0%
! AAGAAGAAGAATGATGAAAA+AGG + chr3_2:65433905-65433924 None:intergenic 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG - chr3_2:65434362-65434381 Msa0417960:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG - chr3_2:65434545-65434564 Msa0417960:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG - chr3_2:65434546-65434565 Msa0417960:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG - chr3_2:65434593-65434612 Msa0417960:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG + chr3_2:65434741-65434760 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG + chr3_2:65434872-65434891 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG + chr3_2:65434873-65434892 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG - chr3_2:65434872-65434891 Msa0417960:CDS 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG - chr3_2:65434916-65434935 Msa0417960:CDS 25.0%
!! TTTTGTGTGGAAATTAAAGA+TGG - chr3_2:65435108-65435127 Msa0417960:five_prime_UTR 25.0%
AAGAAAAGAGTGAAGCTTAA+GGG + chr3_2:65433854-65433873 None:intergenic 30.0%
AGAAGTAAGAAGATGATGTT+AGG - chr3_2:65433973-65433992 Msa0417960:three_prime_UTR 30.0%
GAAGTAAGAAGATGATGTTA+GGG - chr3_2:65433974-65433993 Msa0417960:three_prime_UTR 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG + chr3_2:65434015-65434034 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG + chr3_2:65434084-65434103 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG + chr3_2:65434207-65434226 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG + chr3_2:65434215-65434234 None:intergenic 30.0%
GCATATGAAATATCCAAACA+AGG - chr3_2:65434751-65434770 Msa0417960:CDS 30.0%
TCCAAACAAGGATAAATTGA+TGG - chr3_2:65434763-65434782 Msa0417960:CDS 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG - chr3_2:65434890-65434909 Msa0417960:CDS 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG - chr3_2:65434896-65434915 Msa0417960:CDS 30.0%
! AATTTTTATCTTGGCTAGAG+TGG - chr3_2:65434925-65434944 Msa0417960:CDS 30.0%
CCTATTAAGAATCAAAGAAG+TGG + chr3_2:65434955-65434974 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG - chr3_2:65434952-65434971 Msa0417960:CDS 30.0%
TATGATGACTCAAAATCCAA+TGG + chr3_2:65435008-65435027 None:intergenic 30.0%
TGGAAATTAAAGATGGTGAT+GGG - chr3_2:65435115-65435134 Msa0417960:five_prime_UTR 30.0%
AAGAAAAGAGTGAAGCTTAA+GGG + chr3_2:65433854-65433873 None:intergenic 30.0%
AGAAGTAAGAAGATGATGTT+AGG - chr3_2:65433973-65433992 Msa0417960:three_prime_UTR 30.0%
GAAGTAAGAAGATGATGTTA+GGG - chr3_2:65433974-65433993 Msa0417960:three_prime_UTR 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG + chr3_2:65434015-65434034 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG + chr3_2:65434084-65434103 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG + chr3_2:65434207-65434226 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG + chr3_2:65434215-65434234 None:intergenic 30.0%
GCATATGAAATATCCAAACA+AGG - chr3_2:65434751-65434770 Msa0417960:CDS 30.0%
TCCAAACAAGGATAAATTGA+TGG - chr3_2:65434763-65434782 Msa0417960:CDS 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG - chr3_2:65434890-65434909 Msa0417960:CDS 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG - chr3_2:65434896-65434915 Msa0417960:CDS 30.0%
! AATTTTTATCTTGGCTAGAG+TGG - chr3_2:65434925-65434944 Msa0417960:CDS 30.0%
CCTATTAAGAATCAAAGAAG+TGG + chr3_2:65434955-65434974 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG - chr3_2:65434952-65434971 Msa0417960:CDS 30.0%
TATGATGACTCAAAATCCAA+TGG + chr3_2:65435008-65435027 None:intergenic 30.0%
TGGAAATTAAAGATGGTGAT+GGG - chr3_2:65435115-65435134 Msa0417960:five_prime_UTR 30.0%
! CAGATTCATTCCTCAGTTTT+AGG - chr3_2:65433812-65433831 Msa0417960:three_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG + chr3_2:65433853-65433872 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG + chr3_2:65433855-65433874 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + chr3_2:65433928-65433947 None:intergenic 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG + chr3_2:65434012-65434031 None:intergenic 35.0%
ACTAAGTTTGATACCAGATG+CGG - chr3_2:65434400-65434419 Msa0417960:intron 35.0%
CAGAGACATTTGATACGTTA+GGG + chr3_2:65434449-65434468 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG + chr3_2:65434481-65434500 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG - chr3_2:65434560-65434579 Msa0417960:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG - chr3_2:65434670-65434689 Msa0417960:CDS 35.0%
GCCATCAATTTATCCTTGTT+TGG + chr3_2:65434767-65434786 None:intergenic 35.0%
ATTAATTAGCCATATGTCCC+TGG - chr3_2:65434794-65434813 Msa0417960:CDS 35.0%
TTAATTAGCCATATGTCCCT+GGG - chr3_2:65434795-65434814 Msa0417960:CDS 35.0%
ATAATTTACCCAGGGACATA+TGG + chr3_2:65434806-65434825 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG + chr3_2:65434865-65434884 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG + chr3_2:65434908-65434927 None:intergenic 35.0%
AACAGTTGCTTCAAAGTTAC+TGG + chr3_2:65434978-65434997 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG - chr3_2:65434989-65435008 Msa0417960:five_prime_UTR 35.0%
! CTCATCTCAGCAGTTTTTAA+TGG - chr3_2:65435070-65435089 Msa0417960:five_prime_UTR 35.0%
! ATCTCAGCAGTTTTTAATGG+TGG - chr3_2:65435073-65435092 Msa0417960:five_prime_UTR 35.0%
GTGGAAATTAAAGATGGTGA+TGG - chr3_2:65435114-65435133 Msa0417960:five_prime_UTR 35.0%
!! ATTAAAGATGGTGATGGGTT+TGG - chr3_2:65435120-65435139 Msa0417960:five_prime_UTR 35.0%
!! TTAAAGATGGTGATGGGTTT+GGG - chr3_2:65435121-65435140 Msa0417960:five_prime_UTR 35.0%
! CAGATTCATTCCTCAGTTTT+AGG - chr3_2:65433812-65433831 Msa0417960:three_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG + chr3_2:65433853-65433872 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG + chr3_2:65433855-65433874 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + chr3_2:65433928-65433947 None:intergenic 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG + chr3_2:65434012-65434031 None:intergenic 35.0%
ACTAAGTTTGATACCAGATG+CGG - chr3_2:65434400-65434419 Msa0417960:intron 35.0%
CAGAGACATTTGATACGTTA+GGG + chr3_2:65434449-65434468 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG + chr3_2:65434481-65434500 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG - chr3_2:65434560-65434579 Msa0417960:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG - chr3_2:65434670-65434689 Msa0417960:CDS 35.0%
GCCATCAATTTATCCTTGTT+TGG + chr3_2:65434767-65434786 None:intergenic 35.0%
ATTAATTAGCCATATGTCCC+TGG - chr3_2:65434794-65434813 Msa0417960:CDS 35.0%
TTAATTAGCCATATGTCCCT+GGG - chr3_2:65434795-65434814 Msa0417960:CDS 35.0%
ATAATTTACCCAGGGACATA+TGG + chr3_2:65434806-65434825 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG + chr3_2:65434865-65434884 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG + chr3_2:65434908-65434927 None:intergenic 35.0%
AACAGTTGCTTCAAAGTTAC+TGG + chr3_2:65434978-65434997 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG - chr3_2:65434989-65435008 Msa0417960:five_prime_UTR 35.0%
! CTCATCTCAGCAGTTTTTAA+TGG - chr3_2:65435070-65435089 Msa0417960:five_prime_UTR 35.0%
! ATCTCAGCAGTTTTTAATGG+TGG - chr3_2:65435073-65435092 Msa0417960:five_prime_UTR 35.0%
GTGGAAATTAAAGATGGTGA+TGG - chr3_2:65435114-65435133 Msa0417960:five_prime_UTR 35.0%
!! ATTAAAGATGGTGATGGGTT+TGG - chr3_2:65435120-65435139 Msa0417960:five_prime_UTR 35.0%
!! TTAAAGATGGTGATGGGTTT+GGG - chr3_2:65435121-65435140 Msa0417960:five_prime_UTR 35.0%
TGTTACTGTGTTCAGATCGA+AGG + chr3_2:65434049-65434068 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG - chr3_2:65434093-65434112 Msa0417960:three_prime_UTR 40.0%
! AAGGTTTCTAGGTGATACCA+TGG + chr3_2:65434196-65434215 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG + chr3_2:65434248-65434267 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG + chr3_2:65434249-65434268 None:intergenic 40.0%
GCAGAGACATTTGATACGTT+AGG + chr3_2:65434450-65434469 None:intergenic 40.0%
AAACAAGTTGTGATTGCAGC+TGG - chr3_2:65434701-65434720 Msa0417960:CDS 40.0%
AACAAGTTGTGATTGCAGCT+GGG - chr3_2:65434702-65434721 Msa0417960:CDS 40.0%
TGTTACTGTGTTCAGATCGA+AGG + chr3_2:65434049-65434068 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG - chr3_2:65434093-65434112 Msa0417960:three_prime_UTR 40.0%
! AAGGTTTCTAGGTGATACCA+TGG + chr3_2:65434196-65434215 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG + chr3_2:65434248-65434267 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG + chr3_2:65434249-65434268 None:intergenic 40.0%
GCAGAGACATTTGATACGTT+AGG + chr3_2:65434450-65434469 None:intergenic 40.0%
AAACAAGTTGTGATTGCAGC+TGG - chr3_2:65434701-65434720 Msa0417960:CDS 40.0%
AACAAGTTGTGATTGCAGCT+GGG - chr3_2:65434702-65434721 Msa0417960:CDS 40.0%
! GTTCTTTTCATTGGAGGACG+AGG + chr3_2:65434006-65434025 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG + chr3_2:65434147-65434166 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG - chr3_2:65434147-65434166 Msa0417960:three_prime_UTR 45.0%
!! TGATCTGAACCGTTGATTCC+AGG + chr3_2:65434159-65434178 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG - chr3_2:65434266-65434285 Msa0417960:CDS 45.0%
TACATGTAGATGTCACGACC+AGG + chr3_2:65434294-65434313 None:intergenic 45.0%
! GTTCTTTTCATTGGAGGACG+AGG + chr3_2:65434006-65434025 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG + chr3_2:65434147-65434166 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG - chr3_2:65434147-65434166 Msa0417960:three_prime_UTR 45.0%
!! TGATCTGAACCGTTGATTCC+AGG + chr3_2:65434159-65434178 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG - chr3_2:65434266-65434285 Msa0417960:CDS 45.0%
TACATGTAGATGTCACGACC+AGG + chr3_2:65434294-65434313 None:intergenic 45.0%
CAGGTCCATGACGGTTGAAA+AGG + chr3_2:65434101-65434120 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG - chr3_2:65434099-65434118 Msa0417960:three_prime_UTR 50.0%
! GTTGGTGGATATGGACCGTA+CGG + chr3_2:65434132-65434151 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG + chr3_2:65434141-65434160 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG - chr3_2:65434138-65434157 Msa0417960:three_prime_UTR 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG + chr3_2:65434150-65434169 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG - chr3_2:65434163-65434182 Msa0417960:three_prime_UTR 50.0%
CAACGGTTCAGATCAGAGCA+GGG - chr3_2:65434164-65434183 Msa0417960:three_prime_UTR 50.0%
TCAGAGCAGGGTTATGTCCA+TGG - chr3_2:65434176-65434195 Msa0417960:three_prime_UTR 50.0%
TGTAAACGCCGTTTGGTACC+TGG - chr3_2:65434273-65434292 Msa0417960:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG + chr3_2:65434284-65434303 None:intergenic 50.0%
TACCAGATGCGGAGAATTGC+AGG - chr3_2:65434411-65434430 Msa0417960:intron 50.0%
AACCTGCAATTCTCCGCATC+TGG + chr3_2:65434416-65434435 None:intergenic 50.0%
GATTGCAGCTGGGTCTAAAG+TGG - chr3_2:65434712-65434731 Msa0417960:CDS 50.0%
CAGGTCCATGACGGTTGAAA+AGG + chr3_2:65434101-65434120 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG - chr3_2:65434099-65434118 Msa0417960:three_prime_UTR 50.0%
! GTTGGTGGATATGGACCGTA+CGG + chr3_2:65434132-65434151 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG + chr3_2:65434141-65434160 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG - chr3_2:65434138-65434157 Msa0417960:three_prime_UTR 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG + chr3_2:65434150-65434169 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG - chr3_2:65434163-65434182 Msa0417960:three_prime_UTR 50.0%
CAACGGTTCAGATCAGAGCA+GGG - chr3_2:65434164-65434183 Msa0417960:three_prime_UTR 50.0%
TCAGAGCAGGGTTATGTCCA+TGG - chr3_2:65434176-65434195 Msa0417960:three_prime_UTR 50.0%
TGTAAACGCCGTTTGGTACC+TGG - chr3_2:65434273-65434292 Msa0417960:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG + chr3_2:65434284-65434303 None:intergenic 50.0%
TACCAGATGCGGAGAATTGC+AGG - chr3_2:65434411-65434430 Msa0417960:intron 50.0%
AACCTGCAATTCTCCGCATC+TGG + chr3_2:65434416-65434435 None:intergenic 50.0%
GATTGCAGCTGGGTCTAAAG+TGG - chr3_2:65434712-65434731 Msa0417960:CDS 50.0%
AACCGTCATGGACCTGGTGT+TGG - chr3_2:65434105-65434124 Msa0417960:three_prime_UTR 55.0%
GACCAACACCAGGTCCATGA+CGG + chr3_2:65434110-65434129 None:intergenic 55.0%
AACCGTCATGGACCTGGTGT+TGG - chr3_2:65434105-65434124 Msa0417960:three_prime_UTR 55.0%
GACCAACACCAGGTCCATGA+CGG + chr3_2:65434110-65434129 None:intergenic 55.0%
!! GGACCTGGTGTTGGTCCGTA+CGG - chr3_2:65434114-65434133 Msa0417960:three_prime_UTR 60.0%
!! GGACCTGGTGTTGGTCCGTA+CGG - chr3_2:65434114-65434133 Msa0417960:three_prime_UTR 60.0%
GGACCGTACGGACCAACACC+AGG + chr3_2:65434120-65434139 None:intergenic 65.0%
GGACCGTACGGACCAACACC+AGG + chr3_2:65434120-65434139 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 65433804 65435155 65433804 ID=Msa0417960;Name=Msa0417960
chr3_2 mRNA 65433804 65435155 65433804 ID=Msa0417960-mRNA-1;Parent=Msa0417960;Name=Msa0417960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=181|1|1|1|1|1|2|404|156
chr3_2 exon 65433804 65434346 65433804 ID=Msa0417960-mRNA-1:exon:14747;Parent=Msa0417960-mRNA-1
chr3_2 exon 65434643 65435155 65434643 ID=Msa0417960-mRNA-1:exon:14746;Parent=Msa0417960-mRNA-1
chr3_2 five_prime_UTR 65434975 65435155 65434975 ID=Msa0417960-mRNA-1:five_prime_utr;Parent=Msa0417960-mRNA-1
chr3_2 CDS 65434643 65434974 65434643 ID=Msa0417960-mRNA-1:cds;Parent=Msa0417960-mRNA-1
chr3_2 CDS 65434208 65434346 65434208 ID=Msa0417960-mRNA-1:cds;Parent=Msa0417960-mRNA-1
chr3_2 three_prime_UTR 65433804 65434207 65433804 ID=Msa0417960-mRNA-1:three_prime_utr;Parent=Msa0417960-mRNA-1
Gene Sequence

>Msa0417960

ATGATGTTAGGGAAGAGACCTCGTCCTCCAATGAAAAGAACAACAAGCATGTCAGAGATAACCTTCGATCTGAACACAGTAACAACAGAAGAAGATCCTAACAACAACAACAACCTTTTCAACCGTCATGGACCTGGTGTTGGTCCGTACGGTCCATATCCACCAACTTCTCCTGGAATCAACGGTTCAGATCAGAGCAGGGTTATGTCCATGGTATCACCTAGAAACCTTAGAAGAAATTCAGCTGATATGACTCACAACCCTGATTTCTTACGCTCTTGCTTTCTCTGTAAACGCCGTTTGGTACCTGGTCGTGACATCTACATGTACAAAGGAGATAGTGCTTTCTGCAGTCTAGAGTGTCGTCAACAGCAGATGAATCAAGATGAGAAGAAAGATAAGTGTTCTGTGGTATCAAAGAAACAAGTTGTGATTGCAGCTGGGTCTAAAGTGGCTGCCACAAATTTGTAG
Protein sequence

>Msa0417960

MMLGKRPRPPMKRTTSMSEITFDLNTVTTEEDPNNNNNLFNRHGPGVGPYGPYPPTSPGINGSDQSRVMSMVSPRNLRRNSADMTHNPDFLRSCFLCKRRLVPGRDIYMYKGDSAFCSLECRQQQMNQDEKKDKCSVVSKKQVVIAAGSKVAATNL*