AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0307510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0307510 MtrunA17_Chr3g0115551 99.359 156 1 0 1 156 1 156 1.31e-115 323
Msa0307510 MtrunA17_Chr3g0124361 37.791 172 73 7 1 138 1 172 4.52e-25 95.1
Msa0307510 MtrunA17_Chr2g0295651 45.588 68 35 1 65 132 41 106 1.35e-15 69.3
Msa0307510 MtrunA17_Chr8g0336591 45.312 64 35 0 81 144 29 92 3.23e-15 67.8
Msa0307510 MtrunA17_Chr5g0400051 59.184 49 19 1 86 134 81 128 6.08e-15 68.2
Msa0307510 MtrunA17_Chr4g0067541 58.140 43 18 0 90 132 62 104 1.92e-14 65.5
Msa0307510 MtrunA17_Chr2g0301141 59.574 47 18 1 89 135 185 230 2.08e-13 65.5
Msa0307510 MtrunA17_Chr4g0055191 53.061 49 23 0 86 134 69 117 7.18e-13 62.4
Msa0307510 MtrunA17_Chr4g0070221 44.828 58 31 1 90 147 178 234 5.15e-12 61.6
Msa0307510 MtrunA17_Chr8g0344991 52.273 44 21 0 90 133 39 82 1.93e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0307510 AT1G78020.1 49.375 160 63 7 1 151 1 151 1.60e-41 136
Msa0307510 AT1G22160.1 50.667 150 60 5 1 149 1 137 8.77e-41 134
Msa0307510 AT4G39795.1 38.298 141 64 2 1 138 1 121 7.00e-25 93.2
Msa0307510 AT5G65040.1 37.324 142 56 4 1 142 1 109 1.25e-21 84.3
Msa0307510 AT5G49120.1 50.704 71 27 2 76 142 58 124 2.12e-17 74.7
Msa0307510 AT4G17670.1 60.417 48 19 0 87 134 73 120 8.84e-16 70.5
Msa0307510 AT5G47060.2 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0307510 AT5G47060.1 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0307510 AT1G19200.2 45.679 81 37 3 73 153 149 222 1.71e-13 65.9
Msa0307510 AT2G44670.1 53.191 47 22 0 88 134 14 60 1.87e-13 62.8
Msa0307510 AT1G19200.1 45.679 81 37 3 73 153 136 209 1.89e-13 65.5
Msa0307510 AT1G74940.1 47.143 70 31 3 89 153 149 217 4.89e-13 64.7
Msa0307510 AT5G20700.1 50.000 46 22 1 90 135 182 226 1.09e-11 61.2
Msa0307510 AT1G53885.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0
Msa0307510 AT1G53903.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0

Find 55 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTCTTTGATTCTTAAT+AGG 0.201917 2_4:+422190 Msa0307510:three_prime_UTR
CCAGGAGAAGTTGGTGGATA+TGG 0.255956 2_4:-421371 None:intergenic
GAATTTCTTCTAAGGTTTCT+AGG 0.264307 2_4:-421437 None:intergenic
AACAGTTGCTTCAAAATTAC+TGG 0.268167 2_4:-422213 None:intergenic
ATTAAAATGGTGATGGGTTT+GGG 0.311519 2_4:+422359 Msa0307510:three_prime_UTR
TATCAGCTGAATTTCTTCTA+AGG 0.311688 2_4:-421445 None:intergenic
AGGAAGAAGAATGATGAAAA+AGG 0.318738 2_4:-421132 None:intergenic
AAGCAAGAGCGTAAGAAATC+AGG 0.327281 2_4:-421479 None:intergenic
AATTAAAATGGTGATGGGTT+TGG 0.333198 2_4:+422358 Msa0307510:three_prime_UTR
AGAAGTAGGAAGATGATGTT+AGG 0.345010 2_4:+421206 Msa0307510:exon
GAAGAAAAGAGTGAAGCTTA+AGG 0.359309 2_4:-421080 None:intergenic
GGGTTTGGGAAGTCTTTATA+TGG 0.364716 2_4:+422373 Msa0307510:three_prime_UTR
TGATCTGAACCGTTGATTCC+AGG 0.372333 2_4:-421389 None:intergenic
CTTTCTCTGTAAACGCCGTT+TGG 0.383139 2_4:+421499 Msa0307510:CDS
AAGAAAAGAGTGAAGCTTAA+GGG 0.390991 2_4:-421079 None:intergenic
TGTAAACGCCGTTTGGTACC+TGG 0.425032 2_4:+421506 Msa0307510:CDS
TTTCATATGCTACAAATTTG+TGG 0.444162 2_4:-421976 None:intergenic
ATAATTTACCCAGGGACATA+TGG 0.444772 2_4:-422041 None:intergenic
CCATATCCACCAACTTCTCC+TGG 0.454006 2_4:+421371 Msa0307510:CDS
TCTAAGCAAGGATAAATTGA+TGG 0.454999 2_4:+422001 Msa0307510:three_prime_UTR
AAACAAGTTGTAGTTGCAGC+TGG 0.457073 2_4:+421939 Msa0307510:CDS
CAGGTCCATGACGGTTGAAA+AGG 0.471195 2_4:-421331 None:intergenic
ATTAATTAGCCATATGTCCC+TGG 0.478134 2_4:+422032 Msa0307510:three_prime_UTR
GAAGTAGGAAGATGATGTTA+GGG 0.497228 2_4:+421207 Msa0307510:exon
AACCGTCATGGACCTGGTGT+TGG 0.498303 2_4:+421338 Msa0307510:CDS
AGCAAGAGCGTAAGAAATCA+GGG 0.500012 2_4:-421478 None:intergenic
AGAAAAGAGTGAAGCTTAAG+GGG 0.501406 2_4:-421078 None:intergenic
GGACCTGGTGTTGGTCCGTA+CGG 0.509388 2_4:+421347 Msa0307510:CDS
TCAACGGTTCAGATCAGAGC+AGG 0.509698 2_4:+421396 Msa0307510:CDS
TGTGGAAATTAAAATGGTGA+TGG 0.512294 2_4:+422352 Msa0307510:three_prime_UTR
CCGTTGATTCCAGGAGAAGT+TGG 0.513302 2_4:-421380 None:intergenic
TTTAGTAACACAAAAGAAGT+AGG 0.540706 2_4:+421192 Msa0307510:five_prime_UTR
TGTTACTGTGTTCAGATCGA+AGG 0.548881 2_4:-421279 None:intergenic
TAAATAATAATAATTTACCC+AGG 0.552107 2_4:-422050 None:intergenic
GTGGAAATTAAAATGGTGAT+GGG 0.556951 2_4:+422353 Msa0307510:three_prime_UTR
ATTACAAAGCCAGAACCAAA+AGG 0.558823 2_4:-422143 None:intergenic
TTGATTCCAGGAGAAGTTGG+TGG 0.560507 2_4:-421377 None:intergenic
AAATAATAATAATTTACCCA+GGG 0.565763 2_4:-422049 None:intergenic
CCTATTAAGAATCAAAGAAG+TGG 0.567121 2_4:-422190 None:intergenic
TATGATGACTCAAAATCCAA+TGG 0.572170 2_4:-422243 None:intergenic
AACAAGTTGTAGTTGCAGCT+GGG 0.574251 2_4:+421940 Msa0307510:CDS
TCAGAGCAGGGTTATGTCCA+TGG 0.575433 2_4:+421409 Msa0307510:CDS
AGTTGCAGCTGGGTCTAAAG+TGG 0.592297 2_4:+421950 Msa0307510:CDS
TTAATTAGCCATATGTCCCT+GGG 0.593209 2_4:+422033 Msa0307510:three_prime_UTR
GTTGGTGGATATGGACCGTA+CGG 0.599015 2_4:-421362 None:intergenic
GGACCGTACGGACCAACACC+AGG 0.604586 2_4:-421350 None:intergenic
CCAACTTCTCCTGGAATCAA+CGG 0.605664 2_4:+421380 Msa0307510:CDS
TGTCACGACCAGGTACCAAA+CGG 0.608597 2_4:-421514 None:intergenic
AAGGTTTCTAGGTGATACCA+TGG 0.614252 2_4:-421426 None:intergenic
AAAACAATAACCTAAAACTG+AGG 0.624254 2_4:-421050 None:intergenic
GCATATGAAATATCTAAGCA+AGG 0.626986 2_4:+421989 Msa0307510:exon
GAAGAAAGATAAGTGTTCTG+TGG 0.638421 2_4:+421908 Msa0307510:CDS
GACCAACACCAGGTCCATGA+CGG 0.641553 2_4:-421340 None:intergenic
TACATGTAGATGTCACGACC+AGG 0.649454 2_4:-421524 None:intergenic
CAACGGTTCAGATCAGAGCA+GGG 0.716813 2_4:+421397 Msa0307510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATTATTAATTTTGTG+TGG + chr2_4:421773-421792 Msa0307510:intron 10.0%
!!! TTATTATTATTAATTTTGTG+TGG + chr2_4:421773-421792 Msa0307510:intron 10.0%
!! AAATAATAATAATTTACCCA+GGG - chr2_4:422052-422071 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG - chr2_4:422053-422072 None:intergenic 15.0%
!! AAATAATAATAATTTACCCA+GGG - chr2_4:422052-422071 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG - chr2_4:422053-422072 None:intergenic 15.0%
!!! GATTAATTGTATATTTTGTG+TGG + chr2_4:422334-422353 Msa0307510:three_prime_UTR 20.0%
!!! ATTTTGTGTGGAAATTAAAA+TGG + chr2_4:422346-422365 Msa0307510:three_prime_UTR 20.0%
!!! GATTAATTGTATATTTTGTG+TGG + chr2_4:422334-422353 Msa0307510:three_prime_UTR 20.0%
!!! ATTTTGTGTGGAAATTAAAA+TGG + chr2_4:422346-422365 Msa0307510:three_prime_UTR 20.0%
! AAAACAATAACCTAAAACTG+AGG - chr2_4:421053-421072 None:intergenic 25.0%
! TTTAGTAACACAAAAGAAGT+AGG + chr2_4:421192-421211 Msa0307510:five_prime_UTR 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG + chr2_4:421599-421618 Msa0307510:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG + chr2_4:421783-421802 Msa0307510:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG + chr2_4:421784-421803 Msa0307510:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG + chr2_4:421831-421850 Msa0307510:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG - chr2_4:421979-421998 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG - chr2_4:422110-422129 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG - chr2_4:422111-422130 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG + chr2_4:422110-422129 Msa0307510:three_prime_UTR 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG + chr2_4:422154-422173 Msa0307510:three_prime_UTR 25.0%
! AAAACAATAACCTAAAACTG+AGG - chr2_4:421053-421072 None:intergenic 25.0%
! TTTAGTAACACAAAAGAAGT+AGG + chr2_4:421192-421211 Msa0307510:five_prime_UTR 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG + chr2_4:421599-421618 Msa0307510:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG + chr2_4:421783-421802 Msa0307510:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG + chr2_4:421784-421803 Msa0307510:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG + chr2_4:421831-421850 Msa0307510:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG - chr2_4:421979-421998 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG - chr2_4:422110-422129 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG - chr2_4:422111-422130 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG + chr2_4:422110-422129 Msa0307510:three_prime_UTR 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG + chr2_4:422154-422173 Msa0307510:three_prime_UTR 25.0%
AAGAAAAGAGTGAAGCTTAA+GGG - chr2_4:421082-421101 None:intergenic 30.0%
AGGAAGAAGAATGATGAAAA+AGG - chr2_4:421135-421154 None:intergenic 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG - chr2_4:421248-421267 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG - chr2_4:421317-421336 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG - chr2_4:421440-421459 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG - chr2_4:421448-421467 None:intergenic 30.0%
CAGAAACATTTGATACGTTA+GGG - chr2_4:421686-421705 None:intergenic 30.0%
GCATATGAAATATCTAAGCA+AGG + chr2_4:421989-422008 Msa0307510:exon 30.0%
TCTAAGCAAGGATAAATTGA+TGG + chr2_4:422001-422020 Msa0307510:three_prime_UTR 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG + chr2_4:422128-422147 Msa0307510:three_prime_UTR 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG + chr2_4:422134-422153 Msa0307510:three_prime_UTR 30.0%
! AATTTTTATCTTGGCTAGAG+TGG + chr2_4:422163-422182 Msa0307510:three_prime_UTR 30.0%
CCTATTAAGAATCAAAGAAG+TGG - chr2_4:422193-422212 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG + chr2_4:422190-422209 Msa0307510:three_prime_UTR 30.0%
AACAGTTGCTTCAAAATTAC+TGG - chr2_4:422216-422235 None:intergenic 30.0%
TATGATGACTCAAAATCCAA+TGG - chr2_4:422246-422265 None:intergenic 30.0%
! CTCATCTTAGCAATTTTCAA+TGG + chr2_4:422309-422328 Msa0307510:three_prime_UTR 30.0%
! ATCTTAGCAATTTTCAATGG+TGG + chr2_4:422312-422331 Msa0307510:three_prime_UTR 30.0%
TGTGGAAATTAAAATGGTGA+TGG + chr2_4:422352-422371 Msa0307510:three_prime_UTR 30.0%
GTGGAAATTAAAATGGTGAT+GGG + chr2_4:422353-422372 Msa0307510:three_prime_UTR 30.0%
!! AATTAAAATGGTGATGGGTT+TGG + chr2_4:422358-422377 Msa0307510:three_prime_UTR 30.0%
!! ATTAAAATGGTGATGGGTTT+GGG + chr2_4:422359-422378 Msa0307510:three_prime_UTR 30.0%
AAGAAAAGAGTGAAGCTTAA+GGG - chr2_4:421082-421101 None:intergenic 30.0%
AGGAAGAAGAATGATGAAAA+AGG - chr2_4:421135-421154 None:intergenic 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG - chr2_4:421248-421267 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG - chr2_4:421317-421336 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG - chr2_4:421440-421459 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG - chr2_4:421448-421467 None:intergenic 30.0%
CAGAAACATTTGATACGTTA+GGG - chr2_4:421686-421705 None:intergenic 30.0%
GCATATGAAATATCTAAGCA+AGG + chr2_4:421989-422008 Msa0307510:exon 30.0%
TCTAAGCAAGGATAAATTGA+TGG + chr2_4:422001-422020 Msa0307510:three_prime_UTR 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG + chr2_4:422128-422147 Msa0307510:three_prime_UTR 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG + chr2_4:422134-422153 Msa0307510:three_prime_UTR 30.0%
! AATTTTTATCTTGGCTAGAG+TGG + chr2_4:422163-422182 Msa0307510:three_prime_UTR 30.0%
CCTATTAAGAATCAAAGAAG+TGG - chr2_4:422193-422212 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG + chr2_4:422190-422209 Msa0307510:three_prime_UTR 30.0%
AACAGTTGCTTCAAAATTAC+TGG - chr2_4:422216-422235 None:intergenic 30.0%
TATGATGACTCAAAATCCAA+TGG - chr2_4:422246-422265 None:intergenic 30.0%
! CTCATCTTAGCAATTTTCAA+TGG + chr2_4:422309-422328 Msa0307510:three_prime_UTR 30.0%
! ATCTTAGCAATTTTCAATGG+TGG + chr2_4:422312-422331 Msa0307510:three_prime_UTR 30.0%
TGTGGAAATTAAAATGGTGA+TGG + chr2_4:422352-422371 Msa0307510:three_prime_UTR 30.0%
GTGGAAATTAAAATGGTGAT+GGG + chr2_4:422353-422372 Msa0307510:three_prime_UTR 30.0%
!! AATTAAAATGGTGATGGGTT+TGG + chr2_4:422358-422377 Msa0307510:three_prime_UTR 30.0%
!! ATTAAAATGGTGATGGGTTT+GGG + chr2_4:422359-422378 Msa0307510:three_prime_UTR 30.0%
! CAGATTCATTCCTCAGTTTT+AGG + chr2_4:421040-421059 Msa0307510:five_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG - chr2_4:421081-421100 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG - chr2_4:421083-421102 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG - chr2_4:421158-421177 None:intergenic 35.0%
AGAAGTAGGAAGATGATGTT+AGG + chr2_4:421206-421225 Msa0307510:exon 35.0%
GAAGTAGGAAGATGATGTTA+GGG + chr2_4:421207-421226 Msa0307510:exon 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG - chr2_4:421245-421264 None:intergenic 35.0%
GCAGAAACATTTGATACGTT+AGG - chr2_4:421687-421706 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG - chr2_4:421718-421737 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG + chr2_4:421798-421817 Msa0307510:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG + chr2_4:421908-421927 Msa0307510:CDS 35.0%
ATTAATTAGCCATATGTCCC+TGG + chr2_4:422032-422051 Msa0307510:three_prime_UTR 35.0%
TTAATTAGCCATATGTCCCT+GGG + chr2_4:422033-422052 Msa0307510:three_prime_UTR 35.0%
ATAATTTACCCAGGGACATA+TGG - chr2_4:422044-422063 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG - chr2_4:422103-422122 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG - chr2_4:422146-422165 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG + chr2_4:422227-422246 Msa0307510:three_prime_UTR 35.0%
! CAGATTCATTCCTCAGTTTT+AGG + chr2_4:421040-421059 Msa0307510:five_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG - chr2_4:421081-421100 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG - chr2_4:421083-421102 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG - chr2_4:421158-421177 None:intergenic 35.0%
AGAAGTAGGAAGATGATGTT+AGG + chr2_4:421206-421225 Msa0307510:exon 35.0%
GAAGTAGGAAGATGATGTTA+GGG + chr2_4:421207-421226 Msa0307510:exon 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG - chr2_4:421245-421264 None:intergenic 35.0%
GCAGAAACATTTGATACGTT+AGG - chr2_4:421687-421706 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG - chr2_4:421718-421737 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG + chr2_4:421798-421817 Msa0307510:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG + chr2_4:421908-421927 Msa0307510:CDS 35.0%
ATTAATTAGCCATATGTCCC+TGG + chr2_4:422032-422051 Msa0307510:three_prime_UTR 35.0%
TTAATTAGCCATATGTCCCT+GGG + chr2_4:422033-422052 Msa0307510:three_prime_UTR 35.0%
ATAATTTACCCAGGGACATA+TGG - chr2_4:422044-422063 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG - chr2_4:422103-422122 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG - chr2_4:422146-422165 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG + chr2_4:422227-422246 Msa0307510:three_prime_UTR 35.0%
GAAGAAGAAGAAGAAGAAGG+AGG - chr2_4:421155-421174 None:intergenic 40.0%
TGTTACTGTGTTCAGATCGA+AGG - chr2_4:421282-421301 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG + chr2_4:421326-421345 Msa0307510:CDS 40.0%
! AAGGTTTCTAGGTGATACCA+TGG - chr2_4:421429-421448 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG - chr2_4:421481-421500 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG - chr2_4:421482-421501 None:intergenic 40.0%
ACTAAGTTTGATACCAGACG+CGG + chr2_4:421637-421656 Msa0307510:intron 40.0%
AAACAAGTTGTAGTTGCAGC+TGG + chr2_4:421939-421958 Msa0307510:CDS 40.0%
AACAAGTTGTAGTTGCAGCT+GGG + chr2_4:421940-421959 Msa0307510:CDS 40.0%
GAAGAAGAAGAAGAAGAAGG+AGG - chr2_4:421155-421174 None:intergenic 40.0%
TGTTACTGTGTTCAGATCGA+AGG - chr2_4:421282-421301 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG + chr2_4:421326-421345 Msa0307510:CDS 40.0%
! AAGGTTTCTAGGTGATACCA+TGG - chr2_4:421429-421448 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG - chr2_4:421481-421500 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG - chr2_4:421482-421501 None:intergenic 40.0%
ACTAAGTTTGATACCAGACG+CGG + chr2_4:421637-421656 Msa0307510:intron 40.0%
AAACAAGTTGTAGTTGCAGC+TGG + chr2_4:421939-421958 Msa0307510:CDS 40.0%
AACAAGTTGTAGTTGCAGCT+GGG + chr2_4:421940-421959 Msa0307510:CDS 40.0%
! GTTCTTTTCATTGGAGGACG+AGG - chr2_4:421239-421258 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG - chr2_4:421380-421399 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG + chr2_4:421380-421399 Msa0307510:CDS 45.0%
!! TGATCTGAACCGTTGATTCC+AGG - chr2_4:421392-421411 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG + chr2_4:421499-421518 Msa0307510:CDS 45.0%
TACATGTAGATGTCACGACC+AGG - chr2_4:421527-421546 None:intergenic 45.0%
! GTTCTTTTCATTGGAGGACG+AGG - chr2_4:421239-421258 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG - chr2_4:421380-421399 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG + chr2_4:421380-421399 Msa0307510:CDS 45.0%
!! TGATCTGAACCGTTGATTCC+AGG - chr2_4:421392-421411 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG + chr2_4:421499-421518 Msa0307510:CDS 45.0%
TACATGTAGATGTCACGACC+AGG - chr2_4:421527-421546 None:intergenic 45.0%
CAGGTCCATGACGGTTGAAA+AGG - chr2_4:421334-421353 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG + chr2_4:421332-421351 Msa0307510:CDS 50.0%
! GTTGGTGGATATGGACCGTA+CGG - chr2_4:421365-421384 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG - chr2_4:421374-421393 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG + chr2_4:421371-421390 Msa0307510:CDS 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG - chr2_4:421383-421402 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG + chr2_4:421396-421415 Msa0307510:CDS 50.0%
CAACGGTTCAGATCAGAGCA+GGG + chr2_4:421397-421416 Msa0307510:CDS 50.0%
TCAGAGCAGGGTTATGTCCA+TGG + chr2_4:421409-421428 Msa0307510:CDS 50.0%
TGTAAACGCCGTTTGGTACC+TGG + chr2_4:421506-421525 Msa0307510:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG - chr2_4:421517-421536 None:intergenic 50.0%
AGTTGCAGCTGGGTCTAAAG+TGG + chr2_4:421950-421969 Msa0307510:CDS 50.0%
CAGGTCCATGACGGTTGAAA+AGG - chr2_4:421334-421353 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG + chr2_4:421332-421351 Msa0307510:CDS 50.0%
! GTTGGTGGATATGGACCGTA+CGG - chr2_4:421365-421384 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG - chr2_4:421374-421393 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG + chr2_4:421371-421390 Msa0307510:CDS 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG - chr2_4:421383-421402 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG + chr2_4:421396-421415 Msa0307510:CDS 50.0%
CAACGGTTCAGATCAGAGCA+GGG + chr2_4:421397-421416 Msa0307510:CDS 50.0%
TCAGAGCAGGGTTATGTCCA+TGG + chr2_4:421409-421428 Msa0307510:CDS 50.0%
TGTAAACGCCGTTTGGTACC+TGG + chr2_4:421506-421525 Msa0307510:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG - chr2_4:421517-421536 None:intergenic 50.0%
AGTTGCAGCTGGGTCTAAAG+TGG + chr2_4:421950-421969 Msa0307510:CDS 50.0%
AACCGTCATGGACCTGGTGT+TGG + chr2_4:421338-421357 Msa0307510:CDS 55.0%
GACCAACACCAGGTCCATGA+CGG - chr2_4:421343-421362 None:intergenic 55.0%
TACCAGACGCGGAGAATTGC+AGG + chr2_4:421648-421667 Msa0307510:intron 55.0%
AACCTGCAATTCTCCGCGTC+TGG - chr2_4:421653-421672 None:intergenic 55.0%
AACCGTCATGGACCTGGTGT+TGG + chr2_4:421338-421357 Msa0307510:CDS 55.0%
GACCAACACCAGGTCCATGA+CGG - chr2_4:421343-421362 None:intergenic 55.0%
TACCAGACGCGGAGAATTGC+AGG + chr2_4:421648-421667 Msa0307510:intron 55.0%
AACCTGCAATTCTCCGCGTC+TGG - chr2_4:421653-421672 None:intergenic 55.0%
!! GGACCTGGTGTTGGTCCGTA+CGG + chr2_4:421347-421366 Msa0307510:CDS 60.0%
!! GGACCTGGTGTTGGTCCGTA+CGG + chr2_4:421347-421366 Msa0307510:CDS 60.0%
GGACCGTACGGACCAACACC+AGG - chr2_4:421353-421372 None:intergenic 65.0%
GGACCGTACGGACCAACACC+AGG - chr2_4:421353-421372 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 421032 422393 421032 ID=Msa0307510;Name=Msa0307510
chr2_4 mRNA 421032 422393 421032 ID=Msa0307510-mRNA-1;Parent=Msa0307510;Name=Msa0307510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=186|1|1|1|1|1|2|404|156
chr2_4 exon 421032 421549 421032 ID=Msa0307510-mRNA-1:exon:156;Parent=Msa0307510-mRNA-1
chr2_4 exon 421851 422393 421851 ID=Msa0307510-mRNA-1:exon:157;Parent=Msa0307510-mRNA-1
chr2_4 five_prime_UTR 421032 421217 421032 ID=Msa0307510-mRNA-1:five_prime_utr;Parent=Msa0307510-mRNA-1
chr2_4 CDS 421218 421549 421218 ID=Msa0307510-mRNA-1:cds;Parent=Msa0307510-mRNA-1
chr2_4 CDS 421851 421989 421851 ID=Msa0307510-mRNA-1:cds;Parent=Msa0307510-mRNA-1
chr2_4 three_prime_UTR 421990 422393 421990 ID=Msa0307510-mRNA-1:three_prime_utr;Parent=Msa0307510-mRNA-1
Gene Sequence

>Msa0307510

ATGATGTTAGGGAAGAGACCTCGTCCTCCAATGAAAAGAACAACAAGCATGTCAGAGATAACCTTCGATCTGAACACAGTAACAACAGAAGAAGATCCTAACAACAACAACAACCTTTTCAACCGTCATGGACCTGGTGTTGGTCCGTACGGTCCATATCCACCAACTTCTCCTGGAATCAACGGTTCAGATCAGAGCAGGGTTATGTCCATGGTATCACCTAGAAACCTTAGAAGAAATTCAGCTGATATGACTCACAACCCTGATTTCTTACGCTCTTGCTTTCTCTGTAAACGCCGTTTGGTACCTGGTCGTGACATCTACATGTACAAAGGAGATAGTGCTTTCTGCAGTCTAGAGTGTCGTCAACAGCAGATGAATCAAGATGAGAAGAAAGATAAGTGTTCTGTGGTATCAAAGAAACAAGTTGTAGTTGCAGCTGGGTCTAAAGTGGCTGCCACAAATTTGTAG
Protein sequence

>Msa0307510

MMLGKRPRPPMKRTTSMSEITFDLNTVTTEEDPNNNNNLFNRHGPGVGPYGPYPPTSPGINGSDQSRVMSMVSPRNLRRNSADMTHNPDFLRSCFLCKRRLVPGRDIYMYKGDSAFCSLECRQQQMNQDEKKDKCSVVSKKQVVVAAGSKVAATNL*