AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0370480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0370480 MtrunA17_Chr3g0115551 99.359 156 1 0 1 156 1 156 1.31e-115 323
Msa0370480 MtrunA17_Chr3g0124361 37.791 172 73 7 1 138 1 172 4.52e-25 95.1
Msa0370480 MtrunA17_Chr2g0295651 45.588 68 35 1 65 132 41 106 1.35e-15 69.3
Msa0370480 MtrunA17_Chr8g0336591 45.312 64 35 0 81 144 29 92 3.23e-15 67.8
Msa0370480 MtrunA17_Chr5g0400051 59.184 49 19 1 86 134 81 128 6.08e-15 68.2
Msa0370480 MtrunA17_Chr4g0067541 58.140 43 18 0 90 132 62 104 1.92e-14 65.5
Msa0370480 MtrunA17_Chr2g0301141 59.574 47 18 1 89 135 185 230 2.08e-13 65.5
Msa0370480 MtrunA17_Chr4g0055191 53.061 49 23 0 86 134 69 117 7.18e-13 62.4
Msa0370480 MtrunA17_Chr4g0070221 44.828 58 31 1 90 147 178 234 5.15e-12 61.6
Msa0370480 MtrunA17_Chr8g0344991 52.273 44 21 0 90 133 39 82 1.93e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0370480 AT1G78020.1 49.375 160 63 7 1 151 1 151 1.60e-41 136
Msa0370480 AT1G22160.1 50.667 150 60 5 1 149 1 137 8.77e-41 134
Msa0370480 AT4G39795.1 38.298 141 64 2 1 138 1 121 7.00e-25 93.2
Msa0370480 AT5G65040.1 37.324 142 56 4 1 142 1 109 1.25e-21 84.3
Msa0370480 AT5G49120.1 50.704 71 27 2 76 142 58 124 2.12e-17 74.7
Msa0370480 AT4G17670.1 60.417 48 19 0 87 134 73 120 8.84e-16 70.5
Msa0370480 AT5G47060.2 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0370480 AT5G47060.1 63.830 47 17 0 88 134 95 141 2.09e-15 70.1
Msa0370480 AT1G19200.2 45.679 81 37 3 73 153 149 222 1.71e-13 65.9
Msa0370480 AT2G44670.1 53.191 47 22 0 88 134 14 60 1.87e-13 62.8
Msa0370480 AT1G19200.1 45.679 81 37 3 73 153 136 209 1.89e-13 65.5
Msa0370480 AT1G74940.1 47.143 70 31 3 89 153 149 217 4.89e-13 64.7
Msa0370480 AT5G20700.1 50.000 46 22 1 90 135 182 226 1.09e-11 61.2
Msa0370480 AT1G53885.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0
Msa0370480 AT1G53903.1 50.000 44 22 0 90 133 42 85 7.63e-11 57.0

Find 56 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTCTTTGATTCTTAAT+AGG 0.201917 3_1:-68279147 Msa0370480:three_prime_UTR
CCAGGAGAAGTTGGTGGATA+TGG 0.255956 3_1:+68280294 None:intergenic
GAATTTCTTCTAAGGTTTCT+AGG 0.264307 3_1:+68280228 None:intergenic
AACAGTTGCTTCAAAATTAC+TGG 0.268167 3_1:+68279124 None:intergenic
ATTAAAATGGTGATGGGTTT+GGG 0.311519 3_1:-68278980 Msa0370480:three_prime_UTR
TATCAGCTGAATTTCTTCTA+AGG 0.311688 3_1:+68280220 None:intergenic
AGGAAGAAGAATGATGAAAA+AGG 0.318738 3_1:+68280521 None:intergenic
AAGCAAGAGCGTAAGAAATC+AGG 0.327281 3_1:+68280186 None:intergenic
AGAAGTAAGAAGATGATGTT+AGG 0.329332 3_1:-68280459 Msa0370480:exon
AATTAAAATGGTGATGGGTT+TGG 0.333198 3_1:-68278981 Msa0370480:three_prime_UTR
GAAGAAAAGAGTGAAGCTTA+AGG 0.359309 3_1:+68280573 None:intergenic
GGGTTTGGGAAGTCTTTATA+TGG 0.364716 3_1:-68278966 None:intergenic
TGATCTGAACCGTTGATTCC+AGG 0.372333 3_1:+68280276 None:intergenic
CTTTCTCTGTAAACGCCGTT+TGG 0.383139 3_1:-68280166 Msa0370480:CDS
AAGAAAAGAGTGAAGCTTAA+GGG 0.390991 3_1:+68280574 None:intergenic
TGTAAACGCCGTTTGGTACC+TGG 0.425032 3_1:-68280159 Msa0370480:CDS
TTTCATATGCTACAAATTTG+TGG 0.444162 3_1:+68279361 None:intergenic
ATAATTTACCCAGGGACATA+TGG 0.444772 3_1:+68279296 None:intergenic
CCATATCCACCAACTTCTCC+TGG 0.454006 3_1:-68280294 Msa0370480:CDS
TCTAAGCAAGGATAAATTGA+TGG 0.454999 3_1:-68279336 Msa0370480:three_prime_UTR
AAACAAGTTGTAGTTGCAGC+TGG 0.457073 3_1:-68279398 Msa0370480:CDS
GAAGTAAGAAGATGATGTTA+GGG 0.462099 3_1:-68280458 Msa0370480:exon
CAGGTCCATGACGGTTGAAA+AGG 0.471195 3_1:+68280334 None:intergenic
ATTAATTAGCCATATGTCCC+TGG 0.478134 3_1:-68279305 Msa0370480:three_prime_UTR
AACCGTCATGGACCTGGTGT+TGG 0.498303 3_1:-68280327 Msa0370480:CDS
AGCAAGAGCGTAAGAAATCA+GGG 0.500012 3_1:+68280187 None:intergenic
AGAAAAGAGTGAAGCTTAAG+GGG 0.501406 3_1:+68280575 None:intergenic
GGACCTGGTGTTGGTCCGTA+CGG 0.509388 3_1:-68280318 Msa0370480:CDS
TCAACGGTTCAGATCAGAGC+AGG 0.509698 3_1:-68280269 Msa0370480:CDS
TGTGGAAATTAAAATGGTGA+TGG 0.512294 3_1:-68278987 Msa0370480:three_prime_UTR
CCGTTGATTCCAGGAGAAGT+TGG 0.513302 3_1:+68280285 None:intergenic
TGTTACTGTGTTCAGATCGA+AGG 0.548881 3_1:+68280386 None:intergenic
TAAATAATAATAATTTACCC+AGG 0.552107 3_1:+68279287 None:intergenic
GTGGAAATTAAAATGGTGAT+GGG 0.556951 3_1:-68278986 Msa0370480:three_prime_UTR
ATTACAAAGCCAGAACCAAA+AGG 0.558823 3_1:+68279194 None:intergenic
TTGATTCCAGGAGAAGTTGG+TGG 0.560507 3_1:+68280288 None:intergenic
AAATAATAATAATTTACCCA+GGG 0.565763 3_1:+68279288 None:intergenic
CCTATTAAGAATCAAAGAAG+TGG 0.567121 3_1:+68279147 None:intergenic
TATGATGACTCAAAATCCAA+TGG 0.572170 3_1:+68279094 None:intergenic
AACAAGTTGTAGTTGCAGCT+GGG 0.574251 3_1:-68279397 Msa0370480:CDS
TCAGAGCAGGGTTATGTCCA+TGG 0.575433 3_1:-68280256 Msa0370480:CDS
AGTTGCAGCTGGGTCTAAAG+TGG 0.592297 3_1:-68279387 Msa0370480:CDS
TTAATTAGCCATATGTCCCT+GGG 0.593209 3_1:-68279304 Msa0370480:three_prime_UTR
GTTGGTGGATATGGACCGTA+CGG 0.599015 3_1:+68280303 None:intergenic
GGACCGTACGGACCAACACC+AGG 0.604586 3_1:+68280315 None:intergenic
CCAACTTCTCCTGGAATCAA+CGG 0.605664 3_1:-68280285 Msa0370480:CDS
TGTCACGACCAGGTACCAAA+CGG 0.608597 3_1:+68280151 None:intergenic
AAGGTTTCTAGGTGATACCA+TGG 0.614252 3_1:+68280239 None:intergenic
AAAACAATAACCTAAAACTG+AGG 0.624254 3_1:+68280603 None:intergenic
GCATATGAAATATCTAAGCA+AGG 0.626986 3_1:-68279348 Msa0370480:three_prime_UTR
GAAGAAAGATAAGTGTTCTG+TGG 0.638421 3_1:-68279429 Msa0370480:CDS
GACCAACACCAGGTCCATGA+CGG 0.641553 3_1:+68280325 None:intergenic
TACATGTAGATGTCACGACC+AGG 0.649454 3_1:+68280141 None:intergenic
CACTTGCTTGAAGAAGAAGA+AGG 0.650384 3_1:+68280498 None:intergenic
TTGCTTGAAGAAGAAGAAGG+AGG 0.682502 3_1:+68280501 None:intergenic
CAACGGTTCAGATCAGAGCA+GGG 0.716813 3_1:-68280268 Msa0370480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG - chr3_1:68279502-68279521 Msa0370480:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr3_1:68279503-68279522 Msa0370480:intron 0.0%
!! TATATATATATATATATATA+TGG - chr3_1:68279502-68279521 Msa0370480:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr3_1:68279503-68279522 Msa0370480:intron 0.0%
!! TAGAGATATATATATATATA+GGG + chr3_1:68279829-68279848 None:intergenic 10.0%
!!! TTATTATTATTAATTTTGTG+TGG - chr3_1:68280025-68280044 Msa0370480:intron 10.0%
!! TAGAGATATATATATATATA+GGG + chr3_1:68279829-68279848 None:intergenic 10.0%
!!! TTATTATTATTAATTTTGTG+TGG - chr3_1:68280025-68280044 Msa0370480:intron 10.0%
!! AGAGATATATATATATATAG+GGG + chr3_1:68279828-68279847 None:intergenic 15.0%
!! GTAGAGATATATATATATAT+AGG + chr3_1:68279830-68279849 None:intergenic 15.0%
!! AAATAATAATAATTTACCCA+GGG + chr3_1:68280304-68280323 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG + chr3_1:68280305-68280324 None:intergenic 15.0%
!! AGAGATATATATATATATAG+GGG + chr3_1:68279828-68279847 None:intergenic 15.0%
!! GTAGAGATATATATATATAT+AGG + chr3_1:68279830-68279849 None:intergenic 15.0%
!! AAATAATAATAATTTACCCA+GGG + chr3_1:68280304-68280323 None:intergenic 15.0%
!! TAAATAATAATAATTTACCC+AGG + chr3_1:68280305-68280324 None:intergenic 15.0%
!!! GATTAATTGTATATTTTGTG+TGG - chr3_1:68280584-68280603 Msa0370480:five_prime_UTR 20.0%
!!! ATTTTGTGTGGAAATTAAAA+TGG - chr3_1:68280596-68280615 Msa0370480:five_prime_UTR 20.0%
!!! GATTAATTGTATATTTTGTG+TGG - chr3_1:68280584-68280603 Msa0370480:five_prime_UTR 20.0%
!!! ATTTTGTGTGGAAATTAAAA+TGG - chr3_1:68280596-68280615 Msa0370480:five_prime_UTR 20.0%
! AAAACAATAACCTAAAACTG+AGG + chr3_1:68278989-68279008 None:intergenic 25.0%
! AAGTAGTTAAATACACCTTA+AGG + chr3_1:68279599-68279618 None:intergenic 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG - chr3_1:68279641-68279660 Msa0370480:intron 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG + chr3_1:68279641-68279660 None:intergenic 25.0%
! AAGTTGTTAAATACACCTTA+AGG + chr3_1:68279745-68279764 None:intergenic 25.0%
! ACACCTTAATAAAATCTAGT+GGG - chr3_1:68279788-68279807 Msa0370480:intron 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG - chr3_1:68279852-68279871 Msa0370480:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG - chr3_1:68280035-68280054 Msa0370480:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG - chr3_1:68280036-68280055 Msa0370480:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG - chr3_1:68280083-68280102 Msa0370480:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG + chr3_1:68280231-68280250 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG + chr3_1:68280362-68280381 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG + chr3_1:68280363-68280382 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG - chr3_1:68280362-68280381 Msa0370480:CDS 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG - chr3_1:68280406-68280425 Msa0370480:CDS 25.0%
! AAAACAATAACCTAAAACTG+AGG + chr3_1:68278989-68279008 None:intergenic 25.0%
! AAGTAGTTAAATACACCTTA+AGG + chr3_1:68279599-68279618 None:intergenic 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG - chr3_1:68279641-68279660 Msa0370480:intron 25.0%
!! ACACCTTCTAAAAAATAAGT+GGG + chr3_1:68279641-68279660 None:intergenic 25.0%
! AAGTTGTTAAATACACCTTA+AGG + chr3_1:68279745-68279764 None:intergenic 25.0%
! ACACCTTAATAAAATCTAGT+GGG - chr3_1:68279788-68279807 Msa0370480:intron 25.0%
!!! TTTTTTGCATCTAAAAGTCT+TGG - chr3_1:68279852-68279871 Msa0370480:intron 25.0%
!! TAATTTTGTGTGGATATAGA+AGG - chr3_1:68280035-68280054 Msa0370480:intron 25.0%
!! AATTTTGTGTGGATATAGAA+GGG - chr3_1:68280036-68280055 Msa0370480:intron 25.0%
! GTGAAAAATTAAATTTGCAG+AGG - chr3_1:68280083-68280102 Msa0370480:intron 25.0%
! TTTCATATGCTACAAATTTG+TGG + chr3_1:68280231-68280250 None:intergenic 25.0%
! AGAGTTCATAAAGAAAAAGA+GGG + chr3_1:68280362-68280381 None:intergenic 25.0%
! AAGAGTTCATAAAGAAAAAG+AGG + chr3_1:68280363-68280382 None:intergenic 25.0%
!! TCTTTTTCTTTATGAACTCT+TGG - chr3_1:68280362-68280381 Msa0370480:CDS 25.0%
!!! TGGCTTTGTAATTTTTATCT+TGG - chr3_1:68280406-68280425 Msa0370480:CDS 25.0%
AAGAAAAGAGTGAAGCTTAA+GGG + chr3_1:68279018-68279037 None:intergenic 30.0%
AGGAAGAAGAATGATGAAAA+AGG + chr3_1:68279071-68279090 None:intergenic 30.0%
AGAAGTAAGAAGATGATGTT+AGG - chr3_1:68279130-68279149 Msa0370480:three_prime_UTR 30.0%
GAAGTAAGAAGATGATGTTA+GGG - chr3_1:68279131-68279150 Msa0370480:three_prime_UTR 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG + chr3_1:68279172-68279191 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG + chr3_1:68279241-68279260 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG + chr3_1:68279364-68279383 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG + chr3_1:68279372-68279391 None:intergenic 30.0%
! TTTTAGCAAGTTACACCTTA+AGG - chr3_1:68279581-68279600 Msa0370480:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG - chr3_1:68279640-68279659 Msa0370480:intron 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG + chr3_1:68279700-68279719 None:intergenic 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG - chr3_1:68279700-68279719 Msa0370480:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG + chr3_1:68279640-68279659 None:intergenic 30.0%
! TTTTAGCAACATTCACCTTA+AGG - chr3_1:68279727-68279746 Msa0370480:intron 30.0%
CACACCTTAATAAAATCTAG+TGG - chr3_1:68279787-68279806 Msa0370480:intron 30.0%
! ACACCCACTAGATTTTATTA+AGG + chr3_1:68279794-68279813 None:intergenic 30.0%
! TATATATATAGGGGTTTGCT+AGG + chr3_1:68279819-68279838 None:intergenic 30.0%
GCATATGAAATATCTAAGCA+AGG - chr3_1:68280241-68280260 Msa0370480:CDS 30.0%
TCTAAGCAAGGATAAATTGA+TGG - chr3_1:68280253-68280272 Msa0370480:CDS 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG - chr3_1:68280380-68280399 Msa0370480:CDS 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG - chr3_1:68280386-68280405 Msa0370480:CDS 30.0%
! AATTTTTATCTTGGCTAGAG+TGG - chr3_1:68280415-68280434 Msa0370480:CDS 30.0%
CCTATTAAGAATCAAAGAAG+TGG + chr3_1:68280445-68280464 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG - chr3_1:68280442-68280461 Msa0370480:CDS 30.0%
AACAGTTGCTTCAAAATTAC+TGG + chr3_1:68280468-68280487 None:intergenic 30.0%
TATGATGACTCAAAATCCAA+TGG + chr3_1:68280498-68280517 None:intergenic 30.0%
! CTCATCTTAGCAATTTTCAA+TGG - chr3_1:68280559-68280578 Msa0370480:five_prime_UTR 30.0%
! ATCTTAGCAATTTTCAATGG+TGG - chr3_1:68280562-68280581 Msa0370480:five_prime_UTR 30.0%
TGTGGAAATTAAAATGGTGA+TGG - chr3_1:68280602-68280621 Msa0370480:five_prime_UTR 30.0%
GTGGAAATTAAAATGGTGAT+GGG - chr3_1:68280603-68280622 Msa0370480:five_prime_UTR 30.0%
!! AATTAAAATGGTGATGGGTT+TGG - chr3_1:68280608-68280627 Msa0370480:five_prime_UTR 30.0%
!! ATTAAAATGGTGATGGGTTT+GGG - chr3_1:68280609-68280628 Msa0370480:five_prime_UTR 30.0%
AAGAAAAGAGTGAAGCTTAA+GGG + chr3_1:68279018-68279037 None:intergenic 30.0%
AGGAAGAAGAATGATGAAAA+AGG + chr3_1:68279071-68279090 None:intergenic 30.0%
AGAAGTAAGAAGATGATGTT+AGG - chr3_1:68279130-68279149 Msa0370480:three_prime_UTR 30.0%
GAAGTAAGAAGATGATGTTA+GGG - chr3_1:68279131-68279150 Msa0370480:three_prime_UTR 30.0%
!!! ATGCTTGTTGTTCTTTTCAT+TGG + chr3_1:68279172-68279191 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG + chr3_1:68279241-68279260 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG + chr3_1:68279364-68279383 None:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG + chr3_1:68279372-68279391 None:intergenic 30.0%
! TTTTAGCAAGTTACACCTTA+AGG - chr3_1:68279581-68279600 Msa0370480:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG - chr3_1:68279640-68279659 Msa0370480:intron 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG + chr3_1:68279700-68279719 None:intergenic 30.0%
!!! ACACCCACTTATTTTTTAGA+AGG - chr3_1:68279700-68279719 Msa0370480:intron 30.0%
! CACACCTTCTAAAAAATAAG+TGG + chr3_1:68279640-68279659 None:intergenic 30.0%
! TTTTAGCAACATTCACCTTA+AGG - chr3_1:68279727-68279746 Msa0370480:intron 30.0%
CACACCTTAATAAAATCTAG+TGG - chr3_1:68279787-68279806 Msa0370480:intron 30.0%
! ACACCCACTAGATTTTATTA+AGG + chr3_1:68279794-68279813 None:intergenic 30.0%
! TATATATATAGGGGTTTGCT+AGG + chr3_1:68279819-68279838 None:intergenic 30.0%
GCATATGAAATATCTAAGCA+AGG - chr3_1:68280241-68280260 Msa0370480:CDS 30.0%
TCTAAGCAAGGATAAATTGA+TGG - chr3_1:68280253-68280272 Msa0370480:CDS 30.0%
!!! CTTGGATTTTTTTCTCCTTT+TGG - chr3_1:68280380-68280399 Msa0370480:CDS 30.0%
!!! TTTTTTTCTCCTTTTGGTTC+TGG - chr3_1:68280386-68280405 Msa0370480:CDS 30.0%
! AATTTTTATCTTGGCTAGAG+TGG - chr3_1:68280415-68280434 Msa0370480:CDS 30.0%
CCTATTAAGAATCAAAGAAG+TGG + chr3_1:68280445-68280464 None:intergenic 30.0%
CCACTTCTTTGATTCTTAAT+AGG - chr3_1:68280442-68280461 Msa0370480:CDS 30.0%
AACAGTTGCTTCAAAATTAC+TGG + chr3_1:68280468-68280487 None:intergenic 30.0%
TATGATGACTCAAAATCCAA+TGG + chr3_1:68280498-68280517 None:intergenic 30.0%
! CTCATCTTAGCAATTTTCAA+TGG - chr3_1:68280559-68280578 Msa0370480:five_prime_UTR 30.0%
! ATCTTAGCAATTTTCAATGG+TGG - chr3_1:68280562-68280581 Msa0370480:five_prime_UTR 30.0%
TGTGGAAATTAAAATGGTGA+TGG - chr3_1:68280602-68280621 Msa0370480:five_prime_UTR 30.0%
GTGGAAATTAAAATGGTGAT+GGG - chr3_1:68280603-68280622 Msa0370480:five_prime_UTR 30.0%
!! AATTAAAATGGTGATGGGTT+TGG - chr3_1:68280608-68280627 Msa0370480:five_prime_UTR 30.0%
!! ATTAAAATGGTGATGGGTTT+GGG - chr3_1:68280609-68280628 Msa0370480:five_prime_UTR 30.0%
! CAGATTCATTCCTCAGTTTT+AGG - chr3_1:68278976-68278995 Msa0370480:three_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG + chr3_1:68279017-68279036 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG + chr3_1:68279019-68279038 None:intergenic 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG + chr3_1:68279169-68279188 None:intergenic 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG - chr3_1:68279554-68279573 Msa0370480:intron 35.0%
ACTCCCACATAAAAACTAGT+GGG + chr3_1:68279560-68279579 None:intergenic 35.0%
GTATCCTAGCAAATGTCTAT+AGG - chr3_1:68279665-68279684 Msa0370480:intron 35.0%
AACTCCTATAGACATTTGCT+AGG + chr3_1:68279672-68279691 None:intergenic 35.0%
CAGAGACATTTGATACGTTA+GGG + chr3_1:68279939-68279958 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG + chr3_1:68279971-68279990 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG - chr3_1:68280050-68280069 Msa0370480:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG - chr3_1:68280160-68280179 Msa0370480:CDS 35.0%
ATTAATTAGCCATATGTCCC+TGG - chr3_1:68280284-68280303 Msa0370480:CDS 35.0%
TTAATTAGCCATATGTCCCT+GGG - chr3_1:68280285-68280304 Msa0370480:CDS 35.0%
ATAATTTACCCAGGGACATA+TGG + chr3_1:68280296-68280315 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG + chr3_1:68280355-68280374 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG + chr3_1:68280398-68280417 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG - chr3_1:68280479-68280498 Msa0370480:five_prime_UTR 35.0%
! CAGATTCATTCCTCAGTTTT+AGG - chr3_1:68278976-68278995 Msa0370480:three_prime_UTR 35.0%
AGAAAAGAGTGAAGCTTAAG+GGG + chr3_1:68279017-68279036 None:intergenic 35.0%
GAAGAAAAGAGTGAAGCTTA+AGG + chr3_1:68279019-68279038 None:intergenic 35.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG + chr3_1:68279169-68279188 None:intergenic 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG - chr3_1:68279554-68279573 Msa0370480:intron 35.0%
ACTCCCACATAAAAACTAGT+GGG + chr3_1:68279560-68279579 None:intergenic 35.0%
GTATCCTAGCAAATGTCTAT+AGG - chr3_1:68279665-68279684 Msa0370480:intron 35.0%
AACTCCTATAGACATTTGCT+AGG + chr3_1:68279672-68279691 None:intergenic 35.0%
CAGAGACATTTGATACGTTA+GGG + chr3_1:68279939-68279958 None:intergenic 35.0%
CTTGTGAACACATCTCATAT+CGG + chr3_1:68279971-68279990 None:intergenic 35.0%
! ATAGAAGGGTGTGTGATAAT+TGG - chr3_1:68280050-68280069 Msa0370480:intron 35.0%
GAAGAAAGATAAGTGTTCTG+TGG - chr3_1:68280160-68280179 Msa0370480:CDS 35.0%
ATTAATTAGCCATATGTCCC+TGG - chr3_1:68280284-68280303 Msa0370480:CDS 35.0%
TTAATTAGCCATATGTCCCT+GGG - chr3_1:68280285-68280304 Msa0370480:CDS 35.0%
ATAATTTACCCAGGGACATA+TGG + chr3_1:68280296-68280315 None:intergenic 35.0%
ATAAAGAAAAAGAGGGAGCT+AGG + chr3_1:68280355-68280374 None:intergenic 35.0%
ATTACAAAGCCAGAACCAAA+AGG + chr3_1:68280398-68280417 None:intergenic 35.0%
! GCAACTGTTGTATTTTCCAT+TGG - chr3_1:68280479-68280498 Msa0370480:five_prime_UTR 35.0%
TTGCTTGAAGAAGAAGAAGG+AGG + chr3_1:68279091-68279110 None:intergenic 40.0%
CACTTGCTTGAAGAAGAAGA+AGG + chr3_1:68279094-68279113 None:intergenic 40.0%
TGTTACTGTGTTCAGATCGA+AGG + chr3_1:68279206-68279225 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG - chr3_1:68279250-68279269 Msa0370480:three_prime_UTR 40.0%
! AAGGTTTCTAGGTGATACCA+TGG + chr3_1:68279353-68279372 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG + chr3_1:68279405-68279424 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG + chr3_1:68279406-68279425 None:intergenic 40.0%
!!! CACACCCACTAGTTTTTATG+TGG - chr3_1:68279553-68279572 Msa0370480:intron 40.0%
CACTCCCACATAAAAACTAG+TGG + chr3_1:68279561-68279580 None:intergenic 40.0%
ACTAAGTTTGATACGAGACG+CGG - chr3_1:68279890-68279909 Msa0370480:intron 40.0%
GCAGAGACATTTGATACGTT+AGG + chr3_1:68279940-68279959 None:intergenic 40.0%
AAACAAGTTGTAGTTGCAGC+TGG - chr3_1:68280191-68280210 Msa0370480:CDS 40.0%
AACAAGTTGTAGTTGCAGCT+GGG - chr3_1:68280192-68280211 Msa0370480:CDS 40.0%
TTGCTTGAAGAAGAAGAAGG+AGG + chr3_1:68279091-68279110 None:intergenic 40.0%
CACTTGCTTGAAGAAGAAGA+AGG + chr3_1:68279094-68279113 None:intergenic 40.0%
TGTTACTGTGTTCAGATCGA+AGG + chr3_1:68279206-68279225 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG - chr3_1:68279250-68279269 Msa0370480:three_prime_UTR 40.0%
! AAGGTTTCTAGGTGATACCA+TGG + chr3_1:68279353-68279372 None:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG + chr3_1:68279405-68279424 None:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG + chr3_1:68279406-68279425 None:intergenic 40.0%
!!! CACACCCACTAGTTTTTATG+TGG - chr3_1:68279553-68279572 Msa0370480:intron 40.0%
CACTCCCACATAAAAACTAG+TGG + chr3_1:68279561-68279580 None:intergenic 40.0%
ACTAAGTTTGATACGAGACG+CGG - chr3_1:68279890-68279909 Msa0370480:intron 40.0%
GCAGAGACATTTGATACGTT+AGG + chr3_1:68279940-68279959 None:intergenic 40.0%
AAACAAGTTGTAGTTGCAGC+TGG - chr3_1:68280191-68280210 Msa0370480:CDS 40.0%
AACAAGTTGTAGTTGCAGCT+GGG - chr3_1:68280192-68280211 Msa0370480:CDS 40.0%
! GTTCTTTTCATTGGAGGACG+AGG + chr3_1:68279163-68279182 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG + chr3_1:68279304-68279323 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG - chr3_1:68279304-68279323 Msa0370480:three_prime_UTR 45.0%
!! TGATCTGAACCGTTGATTCC+AGG + chr3_1:68279316-68279335 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG - chr3_1:68279423-68279442 Msa0370480:CDS 45.0%
TACATGTAGATGTCACGACC+AGG + chr3_1:68279451-68279470 None:intergenic 45.0%
! GTTCTTTTCATTGGAGGACG+AGG + chr3_1:68279163-68279182 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG + chr3_1:68279304-68279323 None:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG - chr3_1:68279304-68279323 Msa0370480:three_prime_UTR 45.0%
!! TGATCTGAACCGTTGATTCC+AGG + chr3_1:68279316-68279335 None:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG - chr3_1:68279423-68279442 Msa0370480:CDS 45.0%
TACATGTAGATGTCACGACC+AGG + chr3_1:68279451-68279470 None:intergenic 45.0%
CAGGTCCATGACGGTTGAAA+AGG + chr3_1:68279258-68279277 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG - chr3_1:68279256-68279275 Msa0370480:three_prime_UTR 50.0%
! GTTGGTGGATATGGACCGTA+CGG + chr3_1:68279289-68279308 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG + chr3_1:68279298-68279317 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG - chr3_1:68279295-68279314 Msa0370480:three_prime_UTR 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG + chr3_1:68279307-68279326 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG - chr3_1:68279320-68279339 Msa0370480:three_prime_UTR 50.0%
CAACGGTTCAGATCAGAGCA+GGG - chr3_1:68279321-68279340 Msa0370480:three_prime_UTR 50.0%
TCAGAGCAGGGTTATGTCCA+TGG - chr3_1:68279333-68279352 Msa0370480:three_prime_UTR 50.0%
TGTAAACGCCGTTTGGTACC+TGG - chr3_1:68279430-68279449 Msa0370480:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG + chr3_1:68279441-68279460 None:intergenic 50.0%
AGTTGCAGCTGGGTCTAAAG+TGG - chr3_1:68280202-68280221 Msa0370480:CDS 50.0%
CAGGTCCATGACGGTTGAAA+AGG + chr3_1:68279258-68279277 None:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG - chr3_1:68279256-68279275 Msa0370480:three_prime_UTR 50.0%
! GTTGGTGGATATGGACCGTA+CGG + chr3_1:68279289-68279308 None:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG + chr3_1:68279298-68279317 None:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG - chr3_1:68279295-68279314 Msa0370480:three_prime_UTR 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG + chr3_1:68279307-68279326 None:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG - chr3_1:68279320-68279339 Msa0370480:three_prime_UTR 50.0%
CAACGGTTCAGATCAGAGCA+GGG - chr3_1:68279321-68279340 Msa0370480:three_prime_UTR 50.0%
TCAGAGCAGGGTTATGTCCA+TGG - chr3_1:68279333-68279352 Msa0370480:three_prime_UTR 50.0%
TGTAAACGCCGTTTGGTACC+TGG - chr3_1:68279430-68279449 Msa0370480:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG + chr3_1:68279441-68279460 None:intergenic 50.0%
AGTTGCAGCTGGGTCTAAAG+TGG - chr3_1:68280202-68280221 Msa0370480:CDS 50.0%
AACCGTCATGGACCTGGTGT+TGG - chr3_1:68279262-68279281 Msa0370480:three_prime_UTR 55.0%
GACCAACACCAGGTCCATGA+CGG + chr3_1:68279267-68279286 None:intergenic 55.0%
TACGAGACGCGGAGAATTGC+AGG - chr3_1:68279901-68279920 Msa0370480:intron 55.0%
AACCGTCATGGACCTGGTGT+TGG - chr3_1:68279262-68279281 Msa0370480:three_prime_UTR 55.0%
GACCAACACCAGGTCCATGA+CGG + chr3_1:68279267-68279286 None:intergenic 55.0%
TACGAGACGCGGAGAATTGC+AGG - chr3_1:68279901-68279920 Msa0370480:intron 55.0%
!! GGACCTGGTGTTGGTCCGTA+CGG - chr3_1:68279271-68279290 Msa0370480:three_prime_UTR 60.0%
!! GGACCTGGTGTTGGTCCGTA+CGG - chr3_1:68279271-68279290 Msa0370480:three_prime_UTR 60.0%
GGACCGTACGGACCAACACC+AGG + chr3_1:68279277-68279296 None:intergenic 65.0%
GGACCGTACGGACCAACACC+AGG + chr3_1:68279277-68279296 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 68278968 68280643 68278968 ID=Msa0370480;Name=Msa0370480
chr3_1 mRNA 68278968 68280643 68278968 ID=Msa0370480-mRNA-1;Parent=Msa0370480;Name=Msa0370480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=174|1|1|1|1|1|2|402|156
chr3_1 exon 68278968 68279508 68278968 ID=Msa0370480-mRNA-1:exon:14246;Parent=Msa0370480-mRNA-1
chr3_1 exon 68280138 68280643 68280138 ID=Msa0370480-mRNA-1:exon:14245;Parent=Msa0370480-mRNA-1
chr3_1 five_prime_UTR 68280470 68280643 68280470 ID=Msa0370480-mRNA-1:five_prime_utr;Parent=Msa0370480-mRNA-1
chr3_1 CDS 68280138 68280469 68280138 ID=Msa0370480-mRNA-1:cds;Parent=Msa0370480-mRNA-1
chr3_1 CDS 68279370 68279508 68279370 ID=Msa0370480-mRNA-1:cds;Parent=Msa0370480-mRNA-1
chr3_1 three_prime_UTR 68278968 68279369 68278968 ID=Msa0370480-mRNA-1:three_prime_utr;Parent=Msa0370480-mRNA-1
Gene Sequence

>Msa0370480

ATGATGTTAGGGAAGAGACCTCGTCCTCCAATGAAAAGAACAACAAGCATGTCAGAGATAACCTTCGATCTGAACACAGTAACAACAGAAGAAGATCCTAACAACAACAACAACCTTTTCAACCGTCATGGACCTGGTGTTGGTCCGTACGGTCCATATCCACCAACTTCTCCTGGAATCAACGGTTCAGATCAGAGCAGGGTTATGTCCATGGTATCACCTAGAAACCTTAGAAGAAATTCAGCTGATATGACTCACAACCCTGATTTCTTACGCTCTTGCTTTCTCTGTAAACGCCGTTTGGTACCTGGTCGTGACATCTACATGTACAAAGGAGATAGTGCTTTCTGCAGTCTAGAGTGTCGTCAACAGCAGATGAATCAAGATGAGAAGAAAGATAAGTGTTCTGTGGTATCAAAGAAACAAGTTGTAGTTGCAGCTGGGTCTAAAGTGGCTGCCACAAATTTGTAG
Protein sequence

>Msa0370480

MMLGKRPRPPMKRTTSMSEITFDLNTVTTEEDPNNNNNLFNRHGPGVGPYGPYPPTSPGINGSDQSRVMSMVSPRNLRRNSADMTHNPDFLRSCFLCKRRLVPGRDIYMYKGDSAFCSLECRQQQMNQDEKKDKCSVVSKKQVVVAAGSKVAATNL*