AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466810 MtrunA17_Chr3g0115551 100.000 110 0 0 1 110 1 110 1.65e-79 230
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466810 AT1G78020.1 49.153 118 43 6 1 110 1 109 1.05e-26 97.1
Msa1466810 AT1G22160.1 49.550 111 42 5 1 110 1 98 3.23e-24 90.1

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGGAGAAGTTGGTGGATA+TGG 0.255956 tig0048933:+245 None:intergenic
GAATTTCTTCTAAGGTTTCT+AGG 0.264307 tig0048933:+179 None:intergenic
TATCAGCTGAATTTCTTCTA+AGG 0.311688 tig0048933:+171 None:intergenic
AAGCAAGAGCGTAAGAAATC+AGG 0.327281 tig0048933:+137 None:intergenic
AGAAGTAAGAAGATGATGTT+AGG 0.329332 tig0048933:-410 None:intergenic
TGATCTGAACCGTTGATTCC+AGG 0.372333 tig0048933:+227 Msa1466810:intergenic
CTTTCTCTGTAAACGCCGTT+TGG 0.383139 tig0048933:-117 Msa1466810:CDS
TGTAAACGCCGTTTGGTACC+TGG 0.425032 tig0048933:-110 Msa1466810:CDS
CCATATCCACCAACTTCTCC+TGG 0.454006 tig0048933:-245 Msa1466810:CDS
GAAGTAAGAAGATGATGTTA+GGG 0.462099 tig0048933:-409 None:intergenic
CAGGTCCATGACGGTTGAAA+AGG 0.471195 tig0048933:+285 Msa1466810:intergenic
AACCGTCATGGACCTGGTGT+TGG 0.498303 tig0048933:-278 Msa1466810:CDS
AGCAAGAGCGTAAGAAATCA+GGG 0.500012 tig0048933:+138 Msa1466810:intergenic
GGACCTGGTGTTGGTCCGTA+CGG 0.509388 tig0048933:-269 Msa1466810:CDS
TCAACGGTTCAGATCAGAGC+AGG 0.509698 tig0048933:-220 Msa1466810:CDS
CCGTTGATTCCAGGAGAAGT+TGG 0.513302 tig0048933:+236 Msa1466810:intergenic
TGTTACTGTGTTCAGATCGA+AGG 0.548881 tig0048933:+337 Msa1466810:intergenic
TTGATTCCAGGAGAAGTTGG+TGG 0.560507 tig0048933:+239 Msa1466810:intergenic
TCAGAGCAGGGTTATGTCCA+TGG 0.575433 tig0048933:-207 Msa1466810:CDS
GTTGGTGGATATGGACCGTA+CGG 0.599015 tig0048933:+254 Msa1466810:intergenic
GGACCGTACGGACCAACACC+AGG 0.604586 tig0048933:+266 Msa1466810:intergenic
CCAACTTCTCCTGGAATCAA+CGG 0.605664 tig0048933:-236 Msa1466810:CDS
TGTCACGACCAGGTACCAAA+CGG 0.608597 tig0048933:+102 Msa1466810:intergenic
AAGGTTTCTAGGTGATACCA+TGG 0.614252 tig0048933:+190 Msa1466810:intergenic
GACCAACACCAGGTCCATGA+CGG 0.641553 tig0048933:+276 Msa1466810:intergenic
TACATGTAGATGTCACGACC+AGG 0.649454 tig0048933:+92 Msa1466810:intergenic
CAACGGTTCAGATCAGAGCA+GGG 0.716813 tig0048933:-219 Msa1466810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGCTTGTTGTTCTTTTCAT+TGG + tig0048933:121-140 None:intergenic 30.0%
! AAAAGGTTGTTGTTGTTGTT+AGG + tig0048933:190-209 None:intergenic 30.0%
!! GAATTTCTTCTAAGGTTTCT+AGG + tig0048933:313-332 Msa1466810:intergenic 30.0%
TATCAGCTGAATTTCTTCTA+AGG + tig0048933:321-340 Msa1466810:intergenic 30.0%
!!! CTTGTTGTTCTTTTCATTGG+AGG + tig0048933:118-137 None:intergenic 35.0%
TGTTACTGTGTTCAGATCGA+AGG + tig0048933:155-174 None:intergenic 40.0%
! AACAACCTTTTCAACCGTCA+TGG - tig0048933:199-218 Msa1466810:CDS 40.0%
! AAGGTTTCTAGGTGATACCA+TGG + tig0048933:302-321 Msa1466810:intergenic 40.0%
! AGCAAGAGCGTAAGAAATCA+GGG + tig0048933:354-373 Msa1466810:intergenic 40.0%
! AAGCAAGAGCGTAAGAAATC+AGG + tig0048933:355-374 Msa1466810:intergenic 40.0%
! GTTCTTTTCATTGGAGGACG+AGG + tig0048933:112-131 None:intergenic 45.0%
TTGATTCCAGGAGAAGTTGG+TGG + tig0048933:253-272 Msa1466810:intergenic 45.0%
CCAACTTCTCCTGGAATCAA+CGG - tig0048933:253-272 Msa1466810:CDS 45.0%
!! TGATCTGAACCGTTGATTCC+AGG + tig0048933:265-284 Msa1466810:intergenic 45.0%
CTTTCTCTGTAAACGCCGTT+TGG - tig0048933:372-391 Msa1466810:CDS 45.0%
TACATGTAGATGTCACGACC+AGG + tig0048933:400-419 Msa1466810:intergenic 45.0%
CAGGTCCATGACGGTTGAAA+AGG + tig0048933:207-226 Msa1466810:intergenic 50.0%
! CTTTTCAACCGTCATGGACC+TGG - tig0048933:205-224 Msa1466810:CDS 50.0%
! GTTGGTGGATATGGACCGTA+CGG + tig0048933:238-257 Msa1466810:intergenic 50.0%
!! CCAGGAGAAGTTGGTGGATA+TGG + tig0048933:247-266 Msa1466810:intergenic 50.0%
CCATATCCACCAACTTCTCC+TGG - tig0048933:244-263 Msa1466810:CDS 50.0%
!! CCGTTGATTCCAGGAGAAGT+TGG + tig0048933:256-275 Msa1466810:intergenic 50.0%
TCAACGGTTCAGATCAGAGC+AGG - tig0048933:269-288 Msa1466810:CDS 50.0%
CAACGGTTCAGATCAGAGCA+GGG - tig0048933:270-289 Msa1466810:CDS 50.0%
TCAGAGCAGGGTTATGTCCA+TGG - tig0048933:282-301 Msa1466810:CDS 50.0%
TGTAAACGCCGTTTGGTACC+TGG - tig0048933:379-398 Msa1466810:CDS 50.0%
TGTCACGACCAGGTACCAAA+CGG + tig0048933:390-409 Msa1466810:intergenic 50.0%
AACCGTCATGGACCTGGTGT+TGG - tig0048933:211-230 Msa1466810:CDS 55.0%
GACCAACACCAGGTCCATGA+CGG + tig0048933:216-235 Msa1466810:intergenic 55.0%
!! GGACCTGGTGTTGGTCCGTA+CGG - tig0048933:220-239 Msa1466810:CDS 60.0%
GGACCGTACGGACCAACACC+AGG + tig0048933:226-245 Msa1466810:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0048933 gene 91 420 91 ID=Msa1466810;Name=Msa1466810
tig0048933 mRNA 91 420 91 ID=Msa1466810-mRNA-1;Parent=Msa1466810;Name=Msa1466810-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|110
tig0048933 exon 91 420 91 ID=Msa1466810-mRNA-1:exon:25149;Parent=Msa1466810-mRNA-1
tig0048933 CDS 91 420 91 ID=Msa1466810-mRNA-1:cds;Parent=Msa1466810-mRNA-1
Gene Sequence

>Msa1466810

ATGATGTTAGGGAAGAGACCTCGTCCTCCAATGAAAAGAACAACAAGCATGTCAGAGATAACCTTCGATCTGAACACAGTAACAACAGAAGAAGATCCTAACAACAACAACAACCTTTTCAACCGTCATGGACCTGGTGTTGGTCCGTACGGTCCATATCCACCAACTTCTCCTGGAATCAACGGTTCAGATCAGAGCAGGGTTATGTCCATGGTATCACCTAGAAACCTTAGAAGAAATTCAGCTGATATGACTCACAACCCTGATTTCTTACGCTCTTGCTTTCTCTGTAAACGCCGTTTGGTACCTGGTCGTGACATCTACATGTAC

Protein sequence

>Msa1466810

MMLGKRPRPPMKRTTSMSEITFDLNTVTTEEDPNNNNNLFNRHGPGVGPYGPYPPTSPGINGSDQSRVMSMVSPRNLRRNSADMTHNPDFLRSCFLCKRRLVPGRDIYMY