AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0420110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0420110 MtrunA17_Chr1g0169191 97.159 352 10 0 1 352 1 352 0.0 718
Msa0420110 MtrunA17_Chr3g0118821 96.809 282 9 0 37 318 60 341 0.0 569
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0420110 AT3G58830.1 63.469 271 95 3 81 349 72 340 7.12e-118 345
Msa0420110 AT3G58830.2 63.469 271 95 3 81 349 77 345 7.22e-118 345
Msa0420110 AT3G58830.3 65.823 237 77 3 81 315 77 311 2.06e-105 313

Find 109 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTGTGGTTTCTGGTTTC+TGG 0.057163 3_2:+68374850 None:intergenic
CCCTTCACTGACATTGTTTA+TGG 0.078059 3_2:-68373935 Msa0420110:CDS
TTGATGTTGACTGTGGTTTC+TGG 0.127678 3_2:+68374843 None:intergenic
GATTAAACAAATTTGAAATT+TGG 0.142808 3_2:+68375276 None:intergenic
GGAATGGGTTTCTAACTATT+TGG 0.158847 3_2:-68373907 Msa0420110:CDS
ATAGGCATTCAATATTATTT+TGG 0.183005 3_2:-68375509 Msa0420110:five_prime_UTR
ATATGGGGCAGTGACAATTT+TGG 0.203302 3_2:+68374676 None:intergenic
TCTACGTAGAAAGGGATTTA+AGG 0.208357 3_2:-68374617 Msa0420110:CDS
CAAACCTGCTTAACAATAAA+TGG 0.212685 3_2:+68373857 None:intergenic
GAAGAAATTGAAAAGCATTT+TGG 0.233420 3_2:-68374093 Msa0420110:CDS
TGCCCTCAAAGTTGATTCTT+TGG 0.244850 3_2:+68374731 None:intergenic
TTATATCAGGAACTGAAATA+TGG 0.269807 3_2:+68374659 None:intergenic
GGTAACAAATCTCTTGCTTT+TGG 0.278258 3_2:-68375628 Msa0420110:five_prime_UTR
GGGAATGTGGTACGAGCAAC+TGG 0.332306 3_2:+68375113 None:intergenic
CTATTACATACCTTTCGTAT+AGG 0.335397 3_2:-68375527 Msa0420110:five_prime_UTR
GTATGTAATAGCAATCTTAA+AGG 0.349678 3_2:+68375539 None:intergenic
GAAAGTTGTAAATCAGAGTT+TGG 0.356510 3_2:-68374517 Msa0420110:CDS
TATTTATTACCATTTCCTTT+TGG 0.358685 3_2:-68375399 Msa0420110:five_prime_UTR
TCGTTGGGCATGATGATGTC+TGG 0.366443 3_2:+68375092 None:intergenic
GCCTCCACTTGAGTCTTCAT+TGG 0.369973 3_2:-68374539 Msa0420110:CDS
AGAAGTATTTAATAAGGAAT+AGG 0.381243 3_2:+68374908 None:intergenic
CAGTCAATGTATCTTATATC+AGG 0.381565 3_2:+68374646 None:intergenic
TTTGTCGTTCTGATTTGAAT+TGG 0.389014 3_2:+68374933 None:intergenic
CTCTGTGTCTGAGGAAGACT+TGG 0.390918 3_2:+68375000 None:intergenic
CAAGTGAGAAGTATTTAATA+AGG 0.394930 3_2:+68374902 None:intergenic
TATATCAGGAACTGAAATAT+GGG 0.405912 3_2:+68374660 None:intergenic
GATAATACACATTACCAGTT+TGG 0.409220 3_2:+68373364 None:intergenic
TAATCTGTGTTCACTCAAAA+CGG 0.409941 3_2:-68373296 Msa0420110:three_prime_UTR
GCAGATGTGAAAGCGGCATT+TGG 0.416507 3_2:-68374754 Msa0420110:CDS
AGGAATCTTGAGAGTACAAT+TGG 0.419350 3_2:-68374323 Msa0420110:CDS
GGGCATTCTATGTTCAACTA+TGG 0.421228 3_2:-68374713 Msa0420110:CDS
CTGACATTGTTTATGGCAAT+CGG 0.424991 3_2:-68373928 Msa0420110:CDS
AAGGGCTAGAAAGCAATTAC+AGG 0.425208 3_2:-68374969 Msa0420110:CDS
TAGGATCTGGCAATAGCTTC+TGG 0.429090 3_2:+68373509 None:intergenic
TTGTTTATGGCAATCGGAAT+GGG 0.438839 3_2:-68373922 Msa0420110:CDS
TTACATATGCTCAATAATAC+AGG 0.440183 3_2:+68375750 None:intergenic
CTGATATAAGATACATTGAC+TGG 0.441013 3_2:-68374645 Msa0420110:CDS
TGACTAATATTAATCAACTA+TGG 0.444675 3_2:-68375669 Msa0420110:five_prime_UTR
ATTTCTTCAGCTGTGCCACC+TGG 0.452323 3_2:+68374108 None:intergenic
GGAGCCATTTATTGTTAAGC+AGG 0.454977 3_2:-68373861 Msa0420110:intron
AGAAGTTTCTCAGAGGGACT+TGG 0.459372 3_2:+68375028 None:intergenic
CTGGGCTGCTCTACGTAGAA+AGG 0.459911 3_2:-68374626 Msa0420110:CDS
GGCCAAAGAATCAACTTTGA+GGG 0.460017 3_2:-68374733 Msa0420110:CDS
CTACGTAGAAAGGGATTTAA+GGG 0.460767 3_2:-68374616 Msa0420110:CDS
GGCTCCTCTGCAAGACTCAA+TGG 0.471686 3_2:+68373878 None:intergenic
GTCCCTCTGAGAAACTTCTA+TGG 0.478928 3_2:-68375024 Msa0420110:CDS
AGACAGACAAAGGGAATTCA+AGG 0.479391 3_2:-68374818 Msa0420110:CDS
AGCTGTAGCAACATGTGGTC+AGG 0.481320 3_2:-68375195 Msa0420110:five_prime_UTR
ATACCCCAACCAAAAGGAAA+TGG 0.487139 3_2:+68375390 None:intergenic
TACCCTACTCTTTAACGCTC+TGG 0.487917 3_2:-68374561 Msa0420110:CDS
TGGCCAAAGAATCAACTTTG+AGG 0.489408 3_2:-68374734 Msa0420110:CDS
GTAAAGTACTGGTCTAGAAG+AGG 0.490645 3_2:-68373547 Msa0420110:CDS
TTGACACAATGTCTAGGATC+TGG 0.492519 3_2:+68373496 None:intergenic
AAACTACATTTGTAAAGTAC+TGG 0.493301 3_2:-68373558 Msa0420110:CDS
ATTGTTTATGGCAATCGGAA+TGG 0.494457 3_2:-68373923 Msa0420110:CDS
CCCAGAAAATTAGTATGCTA+TGG 0.495081 3_2:+68373443 None:intergenic
AATCTGTGTTCACTCAAAAC+GGG 0.500997 3_2:-68373295 Msa0420110:three_prime_UTR
GGCCAGAGCGTTAAAGAGTA+GGG 0.506103 3_2:+68374559 None:intergenic
GCTGTATTTAGTAACTCAGC+TGG 0.507393 3_2:-68374484 Msa0420110:intron
GGATTTGAGCTCTATTTACA+AGG 0.512223 3_2:+68375771 None:intergenic
TTATGGTTCTGGATCTAAAG+TGG 0.521729 3_2:-68375605 Msa0420110:five_prime_UTR
TGACTCAACACTAACCTGTC+AGG 0.529657 3_2:+68375861 None:intergenic
CTATGGAGACAAGTCTATCA+AGG 0.534134 3_2:+68373460 None:intergenic
TGGGCTGCTCTACGTAGAAA+GGG 0.534826 3_2:-68374625 Msa0420110:CDS
TTACGCAGGTGATTGATACA+AGG 0.543357 3_2:-68375892 Msa0420110:five_prime_UTR
AGGGACTTGGCTATCTGCAT+TGG 0.544545 3_2:+68375041 None:intergenic
ACATGTATACCCCAACCAAA+AGG 0.548840 3_2:+68375384 None:intergenic
AGGCCAGAGCGTTAAAGAGT+AGG 0.550814 3_2:+68374558 None:intergenic
TTGTTGCAACTCTGTGTCTG+AGG 0.551570 3_2:+68374991 None:intergenic
AACATCAACTAGACAGACAA+AGG 0.555337 3_2:-68374828 Msa0420110:CDS
TAAAGTACTGGTCTAGAAGA+GGG 0.555992 3_2:-68373546 Msa0420110:CDS
GCCATAAACAATGTCAGTGA+AGG 0.556282 3_2:+68373934 None:intergenic
AGACACAGAGTTGCAACAAA+GGG 0.557784 3_2:-68374987 Msa0420110:CDS
CGTTGGGCATGATGATGTCT+GGG 0.560325 3_2:+68375093 None:intergenic
CCATAAACAATGTCAGTGAA+GGG 0.567655 3_2:+68373935 None:intergenic
CATGATGATGTCTGGGAATG+TGG 0.568095 3_2:+68375100 None:intergenic
GGCAATAGCTTCTGGTCAAG+TGG 0.568702 3_2:+68373517 None:intergenic
ACTGCAGGAGTGAAGAAGCC+AGG 0.579048 3_2:-68374126 Msa0420110:intron
CAGACACAGAGTTGCAACAA+AGG 0.579774 3_2:-68374988 Msa0420110:CDS
TCAACTATGGCTATTACTGA+TGG 0.583520 3_2:-68375656 Msa0420110:five_prime_UTR
AGACAAGTCTATCAAGGATG+TGG 0.585793 3_2:+68373466 None:intergenic
ACATCAACTAGACAGACAAA+GGG 0.586910 3_2:-68374827 Msa0420110:CDS
TACGCAGGTGATTGATACAA+GGG 0.588207 3_2:-68375891 Msa0420110:five_prime_UTR
GACCATAGAAGTTTCTCAGA+GGG 0.594094 3_2:+68375022 None:intergenic
TGAAGCATCAGAGTTAATCA+TGG 0.595342 3_2:-68374067 Msa0420110:intron
TGTCTAGTTGATGTTGACTG+TGG 0.599422 3_2:+68374836 None:intergenic
ACAAGGGTAAGTTACCTGAC+AGG 0.607943 3_2:-68375875 Msa0420110:five_prime_UTR
ATATTGAATGCCTATACGAA+AGG 0.615758 3_2:+68375517 None:intergenic
AGTAGACACAACACTAAGCA+AGG 0.618626 3_2:-68375462 Msa0420110:five_prime_UTR
GGCTCTTTGACACAATGTCT+AGG 0.619400 3_2:+68373490 None:intergenic
TTGAGTCATGATTTCACGTG+TGG 0.619429 3_2:-68375846 Msa0420110:five_prime_UTR
CTTTCCAATGAAGACTCAAG+TGG 0.631381 3_2:+68374535 None:intergenic
ACCATGATGGTTCAAAAGCG+AGG 0.633614 3_2:-68374343 Msa0420110:CDS
CTTCGTGAGTATGACCATGA+TGG 0.634215 3_2:-68374356 Msa0420110:CDS
CAAGTCTATCAAGGATGTGG+CGG 0.639966 3_2:+68373469 None:intergenic
AGAGCCATTGAGTCTTGCAG+AGG 0.643454 3_2:-68373882 Msa0420110:CDS
TGATATAAGATACATTGACT+GGG 0.644285 3_2:-68374644 Msa0420110:CDS
TCCAATGAAGACTCAAGTGG+AGG 0.644383 3_2:+68374538 None:intergenic
CATAAACAATGTCAGTGAAG+GGG 0.650053 3_2:+68373936 None:intergenic
ATATCAGGAACTGAAATATG+GGG 0.654119 3_2:+68374661 None:intergenic
TGGCTATTACTGATGGCAAG+TGG 0.656296 3_2:-68375649 Msa0420110:five_prime_UTR
TGCATTGGCAAGTGATAGAG+TGG 0.659077 3_2:+68375056 None:intergenic
CAACTAAAACACATTCCACA+TGG 0.679458 3_2:+68375566 None:intergenic
GGAATGTGGTACGAGCAACT+GGG 0.693669 3_2:+68375114 None:intergenic
TATCGTAATACATGACCATG+TGG 0.700567 3_2:-68375581 Msa0420110:five_prime_UTR
GGACCATAGAAGTTTCTCAG+AGG 0.703711 3_2:+68375021 None:intergenic
CATACAGCTGTAGCAACATG+TGG 0.711649 3_2:-68375200 Msa0420110:five_prime_UTR
GTGGTGGGCAGATGTGAAAG+CGG 0.723068 3_2:-68374761 Msa0420110:CDS
GCAGGAGTGAAGAAGCCAGG+TGG 0.732956 3_2:-68374123 Msa0420110:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATGTATATTTTAAAGT+TGG - chr3_2:68373285-68373304 Msa0420110:three_prime_UTR 15.0%
!!! GAATTTTTTTTTTAATGAGA+AGG - chr3_2:68373828-68373847 Msa0420110:intron 15.0%
!!! GATTAAACAAATTTGAAATT+TGG + chr3_2:68373992-68374011 None:intergenic 15.0%
!! TATGTTAAACCATAAATATT+CGG - chr3_2:68375289-68375308 Msa0420110:five_prime_UTR 15.0%
!! TTCATAAATTAAAATGTACA+AGG + chr3_2:68375583-68375602 None:intergenic 15.0%
!! TGACTAATATTAATCAACTA+TGG - chr3_2:68373596-68373615 Msa0420110:intron 20.0%
!!! ATAGGCATTCAATATTATTT+TGG - chr3_2:68373756-68373775 Msa0420110:intron 20.0%
!!! TATTTATTACCATTTCCTTT+TGG - chr3_2:68373866-68373885 Msa0420110:CDS 20.0%
!!! AGAAGTATTTAATAAGGAAT+AGG + chr3_2:68374360-68374379 None:intergenic 20.0%
!! CAGAAAGAAATGTTTATAAA+TGG + chr3_2:68375641-68375660 None:intergenic 20.0%
!!! TACTATTTTTTTTACCAAAC+TGG - chr3_2:68375887-68375906 Msa0420110:five_prime_UTR 20.0%
!! TATAGAAAAATGAGAAAGTT+GGG - chr3_2:68375993-68376012 Msa0420110:five_prime_UTR 20.0%
!!! TGAAAATTTTGTTTTTACGC+AGG - chr3_2:68373359-68373378 Msa0420110:three_prime_UTR 25.0%
!!! TCCATGATTTTTTTTGTTCA+TGG - chr3_2:68373456-68373475 Msa0420110:three_prime_UTR 25.0%
! ACCATGAACAAAAAAAATCA+TGG + chr3_2:68373460-68373479 None:intergenic 25.0%
! TTACATATGCTCAATAATAC+AGG + chr3_2:68373518-68373537 None:intergenic 25.0%
! GTATGTAATAGCAATCTTAA+AGG + chr3_2:68373729-68373748 None:intergenic 25.0%
!!! AATATTATTTTGGCAACTAC+TGG - chr3_2:68373766-68373785 Msa0420110:intron 25.0%
!!! CAAGTGAGAAGTATTTAATA+AGG + chr3_2:68374366-68374385 None:intergenic 25.0%
! TATATCAGGAACTGAAATAT+GGG + chr3_2:68374608-68374627 None:intergenic 25.0%
! TTATATCAGGAACTGAAATA+TGG + chr3_2:68374609-68374628 None:intergenic 25.0%
!! TGATATAAGATACATTGACT+GGG - chr3_2:68374621-68374640 Msa0420110:CDS 25.0%
!!! TTCAAACTTGTTCTGAAATT+TGG - chr3_2:68374823-68374842 Msa0420110:CDS 25.0%
! TAAGCTTATGTTAAAATGCT+AGG - chr3_2:68375057-68375076 Msa0420110:CDS 25.0%
!!! GAAGAAATTGAAAAGCATTT+TGG - chr3_2:68375172-68375191 Msa0420110:five_prime_UTR 25.0%
!!! TATGTAGTTTGATTTGATTC+AGG - chr3_2:68375264-68375283 Msa0420110:five_prime_UTR 25.0%
!! TTTGGTAAAAGTGTAGTTAA+AGG - chr3_2:68375538-68375557 Msa0420110:five_prime_UTR 25.0%
! AAACTACATTTGTAAAGTAC+TGG - chr3_2:68375707-68375726 Msa0420110:five_prime_UTR 25.0%
! GTATAGAAAAATGAGAAAGT+TGG - chr3_2:68375992-68376011 Msa0420110:five_prime_UTR 25.0%
TACATGAAAAACTGACATGT+AGG + chr3_2:68373274-68373293 None:intergenic 30.0%
!! AAATCTCTTGCTTTTGGTTA+TGG - chr3_2:68373643-68373662 Msa0420110:intron 30.0%
CTATTACATACCTTTCGTAT+AGG - chr3_2:68373738-68373757 Msa0420110:intron 30.0%
ATATTGAATGCCTATACGAA+AGG + chr3_2:68373751-68373770 None:intergenic 30.0%
! TATTACCATTTCCTTTTGGT+TGG - chr3_2:68373870-68373889 Msa0420110:CDS 30.0%
!! ATTACCATTTCCTTTTGGTT+GGG - chr3_2:68373871-68373890 Msa0420110:CDS 30.0%
! TTTGTCGTTCTGATTTGAAT+TGG + chr3_2:68374335-68374354 None:intergenic 30.0%
ATATCAGGAACTGAAATATG+GGG + chr3_2:68374607-68374626 None:intergenic 30.0%
CAGTCAATGTATCTTATATC+AGG + chr3_2:68374622-68374641 None:intergenic 30.0%
CTGATATAAGATACATTGAC+TGG - chr3_2:68374620-68374639 Msa0420110:CDS 30.0%
GAAAGTTGTAAATCAGAGTT+TGG - chr3_2:68374748-68374767 Msa0420110:CDS 30.0%
! TCTTCATTGTTGTTGTAATC+AGG - chr3_2:68374885-68374904 Msa0420110:CDS 30.0%
TAGGATTGTGAAAATTCTGA+GGG - chr3_2:68375076-68375095 Msa0420110:CDS 30.0%
ATACTTTGTATGTATACTGC+AGG - chr3_2:68375124-68375143 Msa0420110:CDS 30.0%
CAAACCTGCTTAACAATAAA+TGG + chr3_2:68375411-68375430 None:intergenic 30.0%
! AGAATGTTTAGAGTGAAGTT+TGG - chr3_2:68375520-68375539 Msa0420110:five_prime_UTR 30.0%
! TCCATAGCATACTAATTTTC+TGG - chr3_2:68375821-68375840 Msa0420110:five_prime_UTR 30.0%
! CCATAGCATACTAATTTTCT+GGG - chr3_2:68375822-68375841 Msa0420110:five_prime_UTR 30.0%
GATAATACACATTACCAGTT+TGG + chr3_2:68375904-68375923 None:intergenic 30.0%
ACTTAACTAACCAAAAAGAC+AGG + chr3_2:68375936-68375955 None:intergenic 30.0%
TAATCTGTGTTCACTCAAAA+CGG - chr3_2:68375969-68375988 Msa0420110:five_prime_UTR 30.0%
GGATTTGAGCTCTATTTACA+AGG + chr3_2:68373497-68373516 None:intergenic 35.0%
TCAACTATGGCTATTACTGA+TGG - chr3_2:68373609-68373628 Msa0420110:intron 35.0%
! GGTAACAAATCTCTTGCTTT+TGG - chr3_2:68373637-68373656 Msa0420110:intron 35.0%
!! TTATGGTTCTGGATCTAAAG+TGG - chr3_2:68373660-68373679 Msa0420110:intron 35.0%
TATCGTAATACATGACCATG+TGG - chr3_2:68373684-68373703 Msa0420110:intron 35.0%
CAACTAAAACACATTCCACA+TGG + chr3_2:68373702-68373721 None:intergenic 35.0%
!! TTACCATTTCCTTTTGGTTG+GGG - chr3_2:68373872-68373891 Msa0420110:CDS 35.0%
!!! GGTTTCTGGATTTTGATTAC+TGG + chr3_2:68374404-68374423 None:intergenic 35.0%
AACATCAACTAGACAGACAA+AGG - chr3_2:68374437-68374456 Msa0420110:intron 35.0%
ACATCAACTAGACAGACAAA+GGG - chr3_2:68374438-68374457 Msa0420110:intron 35.0%
! ACTTTTCACTAAGATGTGGT+GGG - chr3_2:68374489-68374508 Msa0420110:CDS 35.0%
TCTACGTAGAAAGGGATTTA+AGG - chr3_2:68374648-68374667 Msa0420110:CDS 35.0%
CTACGTAGAAAGGGATTTAA+GGG - chr3_2:68374649-68374668 Msa0420110:CDS 35.0%
! AGGAATCTTGAGAGTACAAT+TGG - chr3_2:68374942-68374961 Msa0420110:CDS 35.0%
CTAGGATTGTGAAAATTCTG+AGG - chr3_2:68375075-68375094 Msa0420110:CDS 35.0%
! TGAAGCATCAGAGTTAATCA+TGG - chr3_2:68375198-68375217 Msa0420110:five_prime_UTR 35.0%
AACCATAAATATTCGGATGC+AGG - chr3_2:68375296-68375315 Msa0420110:five_prime_UTR 35.0%
ATAAATATTCGGATGCAGGT+GGG - chr3_2:68375300-68375319 Msa0420110:five_prime_UTR 35.0%
CATAAACAATGTCAGTGAAG+GGG + chr3_2:68375332-68375351 None:intergenic 35.0%
CCATAAACAATGTCAGTGAA+GGG + chr3_2:68375333-68375352 None:intergenic 35.0%
CTGACATTGTTTATGGCAAT+CGG - chr3_2:68375337-68375356 Msa0420110:five_prime_UTR 35.0%
ATTGTTTATGGCAATCGGAA+TGG - chr3_2:68375342-68375361 Msa0420110:five_prime_UTR 35.0%
TTGTTTATGGCAATCGGAAT+GGG - chr3_2:68375343-68375362 Msa0420110:five_prime_UTR 35.0%
! GGAATGGGTTTCTAACTATT+TGG - chr3_2:68375358-68375377 Msa0420110:five_prime_UTR 35.0%
GTTTCAATGATCGTGATGAT+AGG - chr3_2:68375672-68375691 Msa0420110:five_prime_UTR 35.0%
!! TAAAGTACTGGTCTAGAAGA+GGG - chr3_2:68375719-68375738 Msa0420110:five_prime_UTR 35.0%
CCCAGAAAATTAGTATGCTA+TGG + chr3_2:68375825-68375844 None:intergenic 35.0%
AACTAACCAAAAAGACAGGT+TGG + chr3_2:68375932-68375951 None:intergenic 35.0%
AATCTGTGTTCACTCAAAAC+GGG - chr3_2:68375970-68375989 Msa0420110:five_prime_UTR 35.0%
TTACGCAGGTGATTGATACA+AGG - chr3_2:68373373-68373392 Msa0420110:three_prime_UTR 40.0%
TACGCAGGTGATTGATACAA+GGG - chr3_2:68373374-68373393 Msa0420110:three_prime_UTR 40.0%
TTGAGTCATGATTTCACGTG+TGG - chr3_2:68373419-68373438 Msa0420110:three_prime_UTR 40.0%
!! CTTGCTTTTGGTTATGGTTC+TGG - chr3_2:68373649-68373668 Msa0420110:intron 40.0%
AGTAGACACAACACTAAGCA+AGG - chr3_2:68373803-68373822 Msa0420110:intron 40.0%
ATACCCCAACCAAAAGGAAA+TGG + chr3_2:68373878-68373897 None:intergenic 40.0%
ACATGTATACCCCAACCAAA+AGG + chr3_2:68373884-68373903 None:intergenic 40.0%
! GGTGAGTTTTCTCTTTCGTT+GGG + chr3_2:68374191-68374210 None:intergenic 40.0%
! TGGTGAGTTTTCTCTTTCGT+TGG + chr3_2:68374192-68374211 None:intergenic 40.0%
GACCATAGAAGTTTCTCAGA+GGG + chr3_2:68374246-68374265 None:intergenic 40.0%
AGACACAGAGTTGCAACAAA+GGG - chr3_2:68374278-68374297 Msa0420110:intron 40.0%
AAGGGCTAGAAAGCAATTAC+AGG - chr3_2:68374296-68374315 Msa0420110:intron 40.0%
! TTGATGTTGACTGTGGTTTC+TGG + chr3_2:68374425-68374444 None:intergenic 40.0%
! TGTCTAGTTGATGTTGACTG+TGG + chr3_2:68374432-68374451 None:intergenic 40.0%
AGACAGACAAAGGGAATTCA+AGG - chr3_2:68374447-68374466 Msa0420110:intron 40.0%
! GTGCACTTTTCACTAAGATG+TGG - chr3_2:68374485-68374504 Msa0420110:CDS 40.0%
! CACTTTTCACTAAGATGTGG+TGG - chr3_2:68374488-68374507 Msa0420110:CDS 40.0%
TGGCCAAAGAATCAACTTTG+AGG - chr3_2:68374531-68374550 Msa0420110:CDS 40.0%
GGCCAAAGAATCAACTTTGA+GGG - chr3_2:68374532-68374551 Msa0420110:CDS 40.0%
! TGCCCTCAAAGTTGATTCTT+TGG + chr3_2:68374537-68374556 None:intergenic 40.0%
GGGCATTCTATGTTCAACTA+TGG - chr3_2:68374552-68374571 Msa0420110:CDS 40.0%
! TATGGGGCAGTGACAATTTT+GGG + chr3_2:68374591-68374610 None:intergenic 40.0%
! ATATGGGGCAGTGACAATTT+TGG + chr3_2:68374592-68374611 None:intergenic 40.0%
CTTTCCAATGAAGACTCAAG+TGG + chr3_2:68374733-68374752 None:intergenic 40.0%
GCTGTATTTAGTAACTCAGC+TGG - chr3_2:68374781-68374800 Msa0420110:CDS 40.0%
!!! TGAGTGGTGCTTTTTCTGTA+TGG - chr3_2:68374986-68375005 Msa0420110:CDS 40.0%
CACCTGCATCCGAATATTTA+TGG + chr3_2:68375301-68375320 None:intergenic 40.0%
CATAAATATTCGGATGCAGG+TGG - chr3_2:68375299-68375318 Msa0420110:five_prime_UTR 40.0%
CCCTTCACTGACATTGTTTA+TGG - chr3_2:68375330-68375349 Msa0420110:five_prime_UTR 40.0%
GCCATAAACAATGTCAGTGA+AGG + chr3_2:68375334-68375353 None:intergenic 40.0%
GGAGCCATTTATTGTTAAGC+AGG - chr3_2:68375404-68375423 Msa0420110:five_prime_UTR 40.0%
AATGATCGTGATGATAGGTG+AGG - chr3_2:68375677-68375696 Msa0420110:five_prime_UTR 40.0%
!! GTAAAGTACTGGTCTAGAAG+AGG - chr3_2:68375718-68375737 Msa0420110:five_prime_UTR 40.0%
! TTGACACAATGTCTAGGATC+TGG + chr3_2:68375772-68375791 None:intergenic 40.0%
AGACAAGTCTATCAAGGATG+TGG + chr3_2:68375802-68375821 None:intergenic 40.0%
CTATGGAGACAAGTCTATCA+AGG + chr3_2:68375808-68375827 None:intergenic 40.0%
!! AACCAAAAAGACAGGTTGGT+GGG + chr3_2:68375928-68375947 None:intergenic 40.0%
! TAACCAAAAAGACAGGTTGG+TGG + chr3_2:68375929-68375948 None:intergenic 40.0%
ACAAGGGTAAGTTACCTGAC+AGG - chr3_2:68373390-68373409 Msa0420110:three_prime_UTR 45.0%
TGACTCAACACTAACCTGTC+AGG + chr3_2:68373407-68373426 None:intergenic 45.0%
TGGCTATTACTGATGGCAAG+TGG - chr3_2:68373616-68373635 Msa0420110:intron 45.0%
CATACAGCTGTAGCAACATG+TGG - chr3_2:68374065-68374084 Msa0420110:intron 45.0%
! CATGATGATGTCTGGGAATG+TGG + chr3_2:68374168-68374187 None:intergenic 45.0%
! TGCATTGGCAAGTGATAGAG+TGG + chr3_2:68374212-68374231 None:intergenic 45.0%
AGAAGTTTCTCAGAGGGACT+TGG + chr3_2:68374240-68374259 None:intergenic 45.0%
! GTCCCTCTGAGAAACTTCTA+TGG - chr3_2:68374241-68374260 Msa0420110:intron 45.0%
GGACCATAGAAGTTTCTCAG+AGG + chr3_2:68374247-68374266 None:intergenic 45.0%
TTGTTGCAACTCTGTGTCTG+AGG + chr3_2:68374277-68374296 None:intergenic 45.0%
CAGACACAGAGTTGCAACAA+AGG - chr3_2:68374277-68374296 Msa0420110:intron 45.0%
!! TGACTGTGGTTTCTGGTTTC+TGG + chr3_2:68374418-68374437 None:intergenic 45.0%
TACCCTACTCTTTAACGCTC+TGG - chr3_2:68374704-68374723 Msa0420110:CDS 45.0%
TCCAATGAAGACTCAAGTGG+AGG + chr3_2:68374730-68374749 None:intergenic 45.0%
CTTCGTGAGTATGACCATGA+TGG - chr3_2:68374909-68374928 Msa0420110:CDS 45.0%
ACCATGATGGTTCAAAAGCG+AGG - chr3_2:68374922-68374941 Msa0420110:CDS 45.0%
!! TCCTCGCTTTTGAACCATCA+TGG + chr3_2:68374926-68374945 None:intergenic 45.0%
AAGTCATCAGACACAGTGAG+TGG - chr3_2:68374970-68374989 Msa0420110:CDS 45.0%
TAGGATCTGGCAATAGCTTC+TGG + chr3_2:68375759-68375778 None:intergenic 45.0%
GGCTCTTTGACACAATGTCT+AGG + chr3_2:68375778-68375797 None:intergenic 45.0%
CAAGTCTATCAAGGATGTGG+CGG + chr3_2:68375799-68375818 None:intergenic 45.0%
AGCTGTAGCAACATGTGGTC+AGG - chr3_2:68374070-68374089 Msa0420110:CDS 50.0%
GGAATGTGGTACGAGCAACT+GGG + chr3_2:68374154-68374173 None:intergenic 50.0%
! CGTTGGGCATGATGATGTCT+GGG + chr3_2:68374175-68374194 None:intergenic 50.0%
! TCGTTGGGCATGATGATGTC+TGG + chr3_2:68374176-68374195 None:intergenic 50.0%
AGGGACTTGGCTATCTGCAT+TGG + chr3_2:68374227-68374246 None:intergenic 50.0%
CTCTGTGTCTGAGGAAGACT+TGG + chr3_2:68374268-68374287 None:intergenic 50.0%
GCAGATGTGAAAGCGGCATT+TGG - chr3_2:68374511-68374530 Msa0420110:CDS 50.0%
TGGGCTGCTCTACGTAGAAA+GGG - chr3_2:68374640-68374659 Msa0420110:CDS 50.0%
! GGCCAGAGCGTTAAAGAGTA+GGG + chr3_2:68374709-68374728 None:intergenic 50.0%
! AGGCCAGAGCGTTAAAGAGT+AGG + chr3_2:68374710-68374729 None:intergenic 50.0%
GCCTCCACTTGAGTCTTCAT+TGG - chr3_2:68374726-68374745 Msa0420110:CDS 50.0%
ATTTCTTCAGCTGTGCCACC+TGG + chr3_2:68375160-68375179 None:intergenic 50.0%
AGAGCCATTGAGTCTTGCAG+AGG - chr3_2:68375383-68375402 Msa0420110:five_prime_UTR 50.0%
GGCAATAGCTTCTGGTCAAG+TGG + chr3_2:68375751-68375770 None:intergenic 50.0%
! CACCCACCAACCTGTCTTTT+TGG - chr3_2:68375923-68375942 Msa0420110:five_prime_UTR 50.0%
GGGAATGTGGTACGAGCAAC+TGG + chr3_2:68374155-68374174 None:intergenic 55.0%
!! GTGGTGGGCAGATGTGAAAG+CGG - chr3_2:68374504-68374523 Msa0420110:CDS 55.0%
CTGGGCTGCTCTACGTAGAA+AGG - chr3_2:68374639-68374658 Msa0420110:CDS 55.0%
ACTGCAGGAGTGAAGAAGCC+AGG - chr3_2:68375139-68375158 Msa0420110:CDS 55.0%
GGCTCCTCTGCAAGACTCAA+TGG + chr3_2:68375390-68375409 None:intergenic 55.0%
GCAGGAGTGAAGAAGCCAGG+TGG - chr3_2:68375142-68375161 Msa0420110:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 68373227 68376060 68373227 ID=Msa0420110;Name=Msa0420110
chr3_2 mRNA 68373227 68376060 68373227 ID=Msa0420110-mRNA-1;Parent=Msa0420110;Name=Msa0420110-mRNA-1;_AED=0.03;_eAED=0.03;_QI=894|1|1|1|1|1|5|250|352
chr3_2 exon 68373227 68373593 68373227 ID=Msa0420110-mRNA-1:exon:16074;Parent=Msa0420110-mRNA-1
chr3_2 exon 68373862 68373969 68373862 ID=Msa0420110-mRNA-1:exon:16073;Parent=Msa0420110-mRNA-1
chr3_2 exon 68374068 68374141 68374068 ID=Msa0420110-mRNA-1:exon:16072;Parent=Msa0420110-mRNA-1
chr3_2 exon 68374303 68374380 68374303 ID=Msa0420110-mRNA-1:exon:16071;Parent=Msa0420110-mRNA-1
chr3_2 exon 68374485 68376060 68374485 ID=Msa0420110-mRNA-1:exon:16070;Parent=Msa0420110-mRNA-1
chr3_2 five_prime_UTR 68375167 68376060 68375167 ID=Msa0420110-mRNA-1:five_prime_utr;Parent=Msa0420110-mRNA-1
chr3_2 CDS 68374485 68375166 68374485 ID=Msa0420110-mRNA-1:cds;Parent=Msa0420110-mRNA-1
chr3_2 CDS 68374303 68374380 68374303 ID=Msa0420110-mRNA-1:cds;Parent=Msa0420110-mRNA-1
chr3_2 CDS 68374068 68374141 68374068 ID=Msa0420110-mRNA-1:cds;Parent=Msa0420110-mRNA-1
chr3_2 CDS 68373862 68373969 68373862 ID=Msa0420110-mRNA-1:cds;Parent=Msa0420110-mRNA-1
chr3_2 CDS 68373477 68373593 68373477 ID=Msa0420110-mRNA-1:cds;Parent=Msa0420110-mRNA-1
chr3_2 three_prime_UTR 68373227 68373476 68373227 ID=Msa0420110-mRNA-1:three_prime_utr;Parent=Msa0420110-mRNA-1
Gene Sequence

>Msa0420110

ATGCTGTCTACTAGTGCTGCTGCTCAGCTTCCCAGTTGCTCGTACCACATTCCCAGACATCATCATGCCCAACGAAAGAGAAAACTCACCACTCTATCACTTGCCAATGCAGATAGCCAAGTCCCTCTGAGAAACTTCTATGGTCCAAGTCTTCCTCAGACACAGAGTTGCAACAAAGGGCTAGAAAGCAATTACAGGTCTAGATACAATTCCAATTCAAATCAGAACGACAAACACCTATTCCTTATTAAATACTTCTCACTTGCTGACTCAGAAGACACCAGTAATCAAAATCCAGAAACCAGAAACCACAGTCAACATCAACTAGACAGACAAAGGGAATTCAAGGAACACAAAAAAGACAGTGCACTTTTCACTAAGATGTGGTGGGCAGATGTGAAAGCGGCATTTGGCCAAAGAATCAACTTTGAGGGCATTCTATGTTCAACTATGGTCATCTTAAAAGACCCAAAATTGTCACTGCCCCATATTTCAGTTCCTGATATAAGATACATTGACTGGGCTGCTCTACGTAGAAAGGGATTTAAGGGTGTTGTCTTTGACAAAGATAATACTATTACAGTACCCTACTCTTTAACGCTCTGGCCTCCACTTGAGTCTTCATTGGAAAGTTGTAAATCAGAGTTTGGTCAAGATATTGCTGTATTTAGTAACTCAGCTGGACTTCGTGAGTATGACCATGATGGTTCAAAAGCGAGGAATCTTGAGAGTACAATTGGAATTAAAGTCATCAGACACAGAGTGAAGAAGCCAGGTGGCACAGCTGAAGAAATTGAAAAGCATTTTGGCTGTGAAGCATCAGAGTTAATCATGGTGGGTGATCGCCCCTTCACTGACATTGTTTATGGCAATCGGAATGGGTTTCTAACTATTTGGACAGAGCCATTGAGTCTTGCAGAGGAGCCATTTATTGTTAAGCAGGTGAGGAAGCTAGAAACTACATTTGTAAAGTACTGGTCTAGAAGAGGGTTGAAGCCACTTGACCAGAAGCTATTGCCAGATCCTAGACATTGTGTCAAAGAGCCGCCACATCCTTGA
Protein sequence

>Msa0420110

MLSTSAAAQLPSCSYHIPRHHHAQRKRKLTTLSLANADSQVPLRNFYGPSLPQTQSCNKGLESNYRSRYNSNSNQNDKHLFLIKYFSLADSEDTSNQNPETRNHSQHQLDRQREFKEHKKDSALFTKMWWADVKAAFGQRINFEGILCSTMVILKDPKLSLPHISVPDIRYIDWAALRRKGFKGVVFDKDNTITVPYSLTLWPPLESSLESCKSEFGQDIAVFSNSAGLREYDHDGSKARNLESTIGIKVIRHRVKKPGGTAEEIEKHFGCEASELIMVGDRPFTDIVYGNRNGFLTIWTEPLSLAEEPFIVKQVRKLETTFVKYWSRRGLKPLDQKLLPDPRHCVKEPPHP*