AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0511940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511940 MtrunA17_Chr1g0169191 97.159 352 10 0 1 352 1 352 0.0 718
Msa0511940 MtrunA17_Chr3g0118821 96.809 282 9 0 37 318 60 341 0.0 569
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511940 AT3G58830.1 63.469 271 95 3 81 349 72 340 7.12e-118 345
Msa0511940 AT3G58830.2 63.469 271 95 3 81 349 77 345 7.22e-118 345
Msa0511940 AT3G58830.3 65.823 237 77 3 81 315 77 311 2.06e-105 313

Find 115 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTGTGGTTTCTGGTTTC+TGG 0.057163 3_4:+61644684 None:intergenic
CCCTTCACTGACATTGTTTA+TGG 0.078059 3_4:-61643769 Msa0511940:CDS
TTGATGTTGACTGTGGTTTC+TGG 0.127678 3_4:+61644677 None:intergenic
GATTAAACAAATTTGAAATT+TGG 0.142808 3_4:+61645110 None:intergenic
GGAATGGGTTTCTAACTATT+TGG 0.158847 3_4:-61643741 Msa0511940:CDS
ATAGGCATTCAATATTATTT+TGG 0.183005 3_4:-61645343 Msa0511940:five_prime_UTR
ATATGGGGCAGTGACAATTT+TGG 0.203302 3_4:+61644510 None:intergenic
TCTACGTAGAAAGGGATTTA+AGG 0.208357 3_4:-61644451 Msa0511940:CDS
CAAACCTGCTTAACAATAAA+TGG 0.212685 3_4:+61643691 None:intergenic
GAAGAAATTGAAAAGCATTT+TGG 0.233420 3_4:-61643927 Msa0511940:CDS
TGCCCTCAAAGTTGATTCTT+TGG 0.244850 3_4:+61644565 None:intergenic
TTATATCAGGAACTGAAATA+TGG 0.269807 3_4:+61644493 None:intergenic
GGTAACAAATCTCTTGCTTT+TGG 0.278258 3_4:-61645462 Msa0511940:five_prime_UTR
CAGGATAGTGTCTACTCATT+TGG 0.307031 3_4:-61645885 Msa0511940:five_prime_UTR
GGGAATGTGGTACGAGCAAC+TGG 0.332306 3_4:+61644947 None:intergenic
CTATTACATACCTTTCGTAT+AGG 0.335397 3_4:-61645361 Msa0511940:five_prime_UTR
GTATGTAATAGCAATCTTAA+AGG 0.349678 3_4:+61645373 None:intergenic
GAAAGTTGTAAATCAGAGTT+TGG 0.356510 3_4:-61644351 Msa0511940:CDS
TATTTATTACCATTTCCTTT+TGG 0.358685 3_4:-61645233 Msa0511940:five_prime_UTR
TCGTTGGGCATGATGATGTC+TGG 0.366443 3_4:+61644926 None:intergenic
GCCTCCACTTGAGTCTTCAT+TGG 0.369973 3_4:-61644373 Msa0511940:CDS
AGAAGTATTTAATAAGGAAT+AGG 0.381243 3_4:+61644742 None:intergenic
CAGTCAATGTATCTTATATC+AGG 0.381565 3_4:+61644480 None:intergenic
TTTGTCGTTCTGATTTGAAT+TGG 0.389014 3_4:+61644767 None:intergenic
CTCTGTGTCTGAGGAAGACT+TGG 0.390918 3_4:+61644834 None:intergenic
CAAGTGAGAAGTATTTAATA+AGG 0.394930 3_4:+61644736 None:intergenic
TATATCAGGAACTGAAATAT+GGG 0.405912 3_4:+61644494 None:intergenic
GATAATACACATTACCAGTT+TGG 0.409220 3_4:+61643198 None:intergenic
TAATCTGTGTTCACTCAAAA+CGG 0.409941 3_4:-61643130 Msa0511940:three_prime_UTR
GCAGATGTGAAAGCGGCATT+TGG 0.416507 3_4:-61644588 Msa0511940:CDS
AGGAATCTTGAGAGTACAAT+TGG 0.419350 3_4:-61644157 Msa0511940:CDS
GGGCATTCTATGTTCAACTA+TGG 0.421228 3_4:-61644547 Msa0511940:CDS
CTGACATTGTTTATGGCAAT+CGG 0.424991 3_4:-61643762 Msa0511940:CDS
AAGGGCTAGAAAGCAATTAC+AGG 0.425208 3_4:-61644803 Msa0511940:CDS
TAGGATCTGGCAATAGCTTC+TGG 0.429090 3_4:+61643343 None:intergenic
TTGTTTATGGCAATCGGAAT+GGG 0.438839 3_4:-61643756 Msa0511940:CDS
TTACATATGCTCAATAATAC+AGG 0.440183 3_4:+61645584 None:intergenic
CTGATATAAGATACATTGAC+TGG 0.441013 3_4:-61644479 Msa0511940:CDS
TAATTGTTTAGTACCATGAT+AGG 0.442466 3_4:-61645942 Msa0511940:five_prime_UTR
TGACTAATATTAATCAACTA+TGG 0.444675 3_4:-61645503 Msa0511940:five_prime_UTR
ATTTCTTCAGCTGTGCCACC+TGG 0.452323 3_4:+61643942 None:intergenic
GGAGCCATTTATTGTTAAGC+AGG 0.454977 3_4:-61643695 Msa0511940:intron
AGAAGTTTCTCAGAGGGACT+TGG 0.459372 3_4:+61644862 None:intergenic
CTGGGCTGCTCTACGTAGAA+AGG 0.459911 3_4:-61644460 Msa0511940:CDS
GGCCAAAGAATCAACTTTGA+GGG 0.460017 3_4:-61644567 Msa0511940:CDS
CTACGTAGAAAGGGATTTAA+GGG 0.460767 3_4:-61644450 Msa0511940:CDS
TGTTTAGTACCATGATAGGT+AGG 0.463419 3_4:-61645938 Msa0511940:five_prime_UTR
GGCTCCTCTGCAAGACTCAA+TGG 0.471686 3_4:+61643712 None:intergenic
GTCCCTCTGAGAAACTTCTA+TGG 0.478928 3_4:-61644858 Msa0511940:CDS
AGACAGACAAAGGGAATTCA+AGG 0.479391 3_4:-61644652 Msa0511940:CDS
AGCTGTAGCAACATGTGGTC+AGG 0.481320 3_4:-61645029 Msa0511940:five_prime_UTR
ATACCCCAACCAAAAGGAAA+TGG 0.487139 3_4:+61645224 None:intergenic
TACCCTACTCTTTAACGCTC+TGG 0.487917 3_4:-61644395 Msa0511940:CDS
CTAGGCAACCCTACCTATCA+TGG 0.489163 3_4:+61645929 None:intergenic
TGGCCAAAGAATCAACTTTG+AGG 0.489408 3_4:-61644568 Msa0511940:CDS
GTAAAGTACTGGTCTAGAAG+AGG 0.490645 3_4:-61643381 Msa0511940:CDS
TTGACACAATGTCTAGGATC+TGG 0.492519 3_4:+61643330 None:intergenic
AAACTACATTTGTAAAGTAC+TGG 0.493301 3_4:-61643392 Msa0511940:CDS
ATTGTTTATGGCAATCGGAA+TGG 0.494457 3_4:-61643757 Msa0511940:CDS
CCCAGAAAATTAGTATGCTA+TGG 0.495081 3_4:+61643277 None:intergenic
AATCTGTGTTCACTCAAAAC+GGG 0.500997 3_4:-61643129 Msa0511940:three_prime_UTR
GGCCAGAGCGTTAAAGAGTA+GGG 0.506103 3_4:+61644393 None:intergenic
GCTGTATTTAGTAACTCAGC+TGG 0.507393 3_4:-61644318 Msa0511940:intron
GGATTTGAGCTCTATTTACA+AGG 0.512223 3_4:+61645605 None:intergenic
TTATGGTTCTGGATCTAAAG+TGG 0.521729 3_4:-61645439 Msa0511940:five_prime_UTR
TGACTCAACACTAACCTGTC+AGG 0.529657 3_4:+61645695 None:intergenic
CTATGGAGACAAGTCTATCA+AGG 0.534134 3_4:+61643294 None:intergenic
TGGGCTGCTCTACGTAGAAA+GGG 0.534826 3_4:-61644459 Msa0511940:CDS
GTTTAGTACCATGATAGGTA+GGG 0.541249 3_4:-61645937 Msa0511940:five_prime_UTR
TTACGCAGGTGATTGATACA+AGG 0.543357 3_4:-61645726 Msa0511940:five_prime_UTR
AGGGACTTGGCTATCTGCAT+TGG 0.544545 3_4:+61644875 None:intergenic
ACATGTATACCCCAACCAAA+AGG 0.548840 3_4:+61645218 None:intergenic
AGGCCAGAGCGTTAAAGAGT+AGG 0.550814 3_4:+61644392 None:intergenic
TTGTTGCAACTCTGTGTCTG+AGG 0.551570 3_4:+61644825 None:intergenic
AACATCAACTAGACAGACAA+AGG 0.555337 3_4:-61644662 Msa0511940:CDS
TAAAGTACTGGTCTAGAAGA+GGG 0.555992 3_4:-61643380 Msa0511940:CDS
GCCATAAACAATGTCAGTGA+AGG 0.556282 3_4:+61643768 None:intergenic
AGACACAGAGTTGCAACAAA+GGG 0.557784 3_4:-61644821 Msa0511940:CDS
CGTTGGGCATGATGATGTCT+GGG 0.560325 3_4:+61644927 None:intergenic
CCATAAACAATGTCAGTGAA+GGG 0.567655 3_4:+61643769 None:intergenic
CATGATGATGTCTGGGAATG+TGG 0.568095 3_4:+61644934 None:intergenic
GGCAATAGCTTCTGGTCAAG+TGG 0.568702 3_4:+61643351 None:intergenic
ACTGCAGGAGTGAAGAAGCC+AGG 0.579048 3_4:-61643960 Msa0511940:intron
CAGACACAGAGTTGCAACAA+AGG 0.579774 3_4:-61644822 Msa0511940:CDS
TCAACTATGGCTATTACTGA+TGG 0.583520 3_4:-61645490 Msa0511940:five_prime_UTR
AGACAAGTCTATCAAGGATG+TGG 0.585793 3_4:+61643300 None:intergenic
ACATCAACTAGACAGACAAA+GGG 0.586910 3_4:-61644661 Msa0511940:CDS
TACGCAGGTGATTGATACAA+GGG 0.588207 3_4:-61645725 Msa0511940:five_prime_UTR
GACCATAGAAGTTTCTCAGA+GGG 0.594094 3_4:+61644856 None:intergenic
TGAAGCATCAGAGTTAATCA+TGG 0.595342 3_4:-61643901 Msa0511940:intron
TGTCTAGTTGATGTTGACTG+TGG 0.599422 3_4:+61644670 None:intergenic
AGACACTATCCTGCAACCCA+TGG 0.601532 3_4:+61645895 None:intergenic
ACAAGGGTAAGTTACCTGAC+AGG 0.607943 3_4:-61645709 Msa0511940:five_prime_UTR
ATATTGAATGCCTATACGAA+AGG 0.615758 3_4:+61645351 None:intergenic
AGTAGACACAACACTAAGCA+AGG 0.618626 3_4:-61645296 Msa0511940:five_prime_UTR
GGCTCTTTGACACAATGTCT+AGG 0.619400 3_4:+61643324 None:intergenic
TTGAGTCATGATTTCACGTG+TGG 0.619429 3_4:-61645680 Msa0511940:five_prime_UTR
CTTTCCAATGAAGACTCAAG+TGG 0.631381 3_4:+61644369 None:intergenic
ACCATGATGGTTCAAAAGCG+AGG 0.633614 3_4:-61644177 Msa0511940:CDS
CTTCGTGAGTATGACCATGA+TGG 0.634215 3_4:-61644190 Msa0511940:CDS
CAAGTCTATCAAGGATGTGG+CGG 0.639966 3_4:+61643303 None:intergenic
AGAGCCATTGAGTCTTGCAG+AGG 0.643454 3_4:-61643716 Msa0511940:CDS
TGATATAAGATACATTGACT+GGG 0.644285 3_4:-61644478 Msa0511940:CDS
TCCAATGAAGACTCAAGTGG+AGG 0.644383 3_4:+61644372 None:intergenic
CATAAACAATGTCAGTGAAG+GGG 0.650053 3_4:+61643770 None:intergenic
ATATCAGGAACTGAAATATG+GGG 0.654119 3_4:+61644495 None:intergenic
TGGCTATTACTGATGGCAAG+TGG 0.656296 3_4:-61645483 Msa0511940:five_prime_UTR
TGCATTGGCAAGTGATAGAG+TGG 0.659077 3_4:+61644890 None:intergenic
CAACTAAAACACATTCCACA+TGG 0.679458 3_4:+61645400 None:intergenic
GGAATGTGGTACGAGCAACT+GGG 0.693669 3_4:+61644948 None:intergenic
TATCGTAATACATGACCATG+TGG 0.700567 3_4:-61645415 Msa0511940:five_prime_UTR
GGACCATAGAAGTTTCTCAG+AGG 0.703711 3_4:+61644855 None:intergenic
CATACAGCTGTAGCAACATG+TGG 0.711649 3_4:-61645034 Msa0511940:five_prime_UTR
GTGGTGGGCAGATGTGAAAG+CGG 0.723068 3_4:-61644595 Msa0511940:CDS
GCAGGAGTGAAGAAGCCAGG+TGG 0.732956 3_4:-61643957 Msa0511940:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATGTATATTTTAAAGT+TGG - chr3_4:61643252-61643271 Msa0511940:three_prime_UTR 15.0%
!!! GAATTTTTTTTTTAATGAGA+AGG - chr3_4:61643795-61643814 Msa0511940:intron 15.0%
!!! GATTAAACAAATTTGAAATT+TGG + chr3_4:61643959-61643978 None:intergenic 15.0%
!! TATGTTAAACCATAAATATT+CGG - chr3_4:61645256-61645275 Msa0511940:five_prime_UTR 15.0%
!! TTCATAAATTAAAATGTACA+AGG + chr3_4:61645550-61645569 None:intergenic 15.0%
!! TGACTAATATTAATCAACTA+TGG - chr3_4:61643563-61643582 Msa0511940:intron 20.0%
!!! ATAGGCATTCAATATTATTT+TGG - chr3_4:61643723-61643742 Msa0511940:CDS 20.0%
!!! TATTTATTACCATTTCCTTT+TGG - chr3_4:61643833-61643852 Msa0511940:intron 20.0%
!!! AGAAGTATTTAATAAGGAAT+AGG + chr3_4:61644327-61644346 None:intergenic 20.0%
!! CAGAAAGAAATGTTTATAAA+TGG + chr3_4:61645608-61645627 None:intergenic 20.0%
!!! TACTATTTTTTTTACCAAAC+TGG - chr3_4:61645854-61645873 Msa0511940:five_prime_UTR 20.0%
!! TATAGAAAAATGAGAAAGTT+GGG - chr3_4:61645960-61645979 Msa0511940:five_prime_UTR 20.0%
! TAATTGTTTAGTACCATGAT+AGG - chr3_4:61643124-61643143 Msa0511940:three_prime_UTR 25.0%
!!! TGAAAATTTTGTTTTTACGC+AGG - chr3_4:61643326-61643345 Msa0511940:CDS 25.0%
!!! TCCATGATTTTTTTTGTTCA+TGG - chr3_4:61643423-61643442 Msa0511940:intron 25.0%
! ACCATGAACAAAAAAAATCA+TGG + chr3_4:61643427-61643446 None:intergenic 25.0%
! TTACATATGCTCAATAATAC+AGG + chr3_4:61643485-61643504 None:intergenic 25.0%
! GTATGTAATAGCAATCTTAA+AGG + chr3_4:61643696-61643715 None:intergenic 25.0%
!!! AATATTATTTTGGCAACTAC+TGG - chr3_4:61643733-61643752 Msa0511940:CDS 25.0%
!!! CAAGTGAGAAGTATTTAATA+AGG + chr3_4:61644333-61644352 None:intergenic 25.0%
! TATATCAGGAACTGAAATAT+GGG + chr3_4:61644575-61644594 None:intergenic 25.0%
! TTATATCAGGAACTGAAATA+TGG + chr3_4:61644576-61644595 None:intergenic 25.0%
!! TGATATAAGATACATTGACT+GGG - chr3_4:61644588-61644607 Msa0511940:CDS 25.0%
!!! TTCAAACTTGTTCTGAAATT+TGG - chr3_4:61644790-61644809 Msa0511940:CDS 25.0%
! TAAGCTTATGTTAAAATGCT+AGG - chr3_4:61645024-61645043 Msa0511940:five_prime_UTR 25.0%
!!! GAAGAAATTGAAAAGCATTT+TGG - chr3_4:61645139-61645158 Msa0511940:five_prime_UTR 25.0%
!!! TATGTAGTTTGATTTGATTC+AGG - chr3_4:61645231-61645250 Msa0511940:five_prime_UTR 25.0%
!! TTTGGTAAAAGTGTAGTTAA+AGG - chr3_4:61645505-61645524 Msa0511940:five_prime_UTR 25.0%
! AAACTACATTTGTAAAGTAC+TGG - chr3_4:61645674-61645693 Msa0511940:five_prime_UTR 25.0%
! GTATAGAAAAATGAGAAAGT+TGG - chr3_4:61645959-61645978 Msa0511940:five_prime_UTR 25.0%
TTCATCTAAAAATCCTCACA+TGG - chr3_4:61643081-61643100 Msa0511940:three_prime_UTR 30.0%
TACATGAAAAACTGACATGT+AGG + chr3_4:61643241-61643260 None:intergenic 30.0%
!! AAATCTCTTGCTTTTGGTTA+TGG - chr3_4:61643610-61643629 Msa0511940:intron 30.0%
CTATTACATACCTTTCGTAT+AGG - chr3_4:61643705-61643724 Msa0511940:CDS 30.0%
ATATTGAATGCCTATACGAA+AGG + chr3_4:61643718-61643737 None:intergenic 30.0%
! TATTACCATTTCCTTTTGGT+TGG - chr3_4:61643837-61643856 Msa0511940:intron 30.0%
!! ATTACCATTTCCTTTTGGTT+GGG - chr3_4:61643838-61643857 Msa0511940:intron 30.0%
! TTTGTCGTTCTGATTTGAAT+TGG + chr3_4:61644302-61644321 None:intergenic 30.0%
ATATCAGGAACTGAAATATG+GGG + chr3_4:61644574-61644593 None:intergenic 30.0%
CAGTCAATGTATCTTATATC+AGG + chr3_4:61644589-61644608 None:intergenic 30.0%
CTGATATAAGATACATTGAC+TGG - chr3_4:61644587-61644606 Msa0511940:CDS 30.0%
GAAAGTTGTAAATCAGAGTT+TGG - chr3_4:61644715-61644734 Msa0511940:CDS 30.0%
! TCTTCATTGTTGTTGTAATC+AGG - chr3_4:61644852-61644871 Msa0511940:CDS 30.0%
TAGGATTGTGAAAATTCTGA+GGG - chr3_4:61645043-61645062 Msa0511940:five_prime_UTR 30.0%
ATACTTTGTATGTATACTGC+AGG - chr3_4:61645091-61645110 Msa0511940:five_prime_UTR 30.0%
CAAACCTGCTTAACAATAAA+TGG + chr3_4:61645378-61645397 None:intergenic 30.0%
! AGAATGTTTAGAGTGAAGTT+TGG - chr3_4:61645487-61645506 Msa0511940:five_prime_UTR 30.0%
! TCCATAGCATACTAATTTTC+TGG - chr3_4:61645788-61645807 Msa0511940:five_prime_UTR 30.0%
! CCATAGCATACTAATTTTCT+GGG - chr3_4:61645789-61645808 Msa0511940:five_prime_UTR 30.0%
GATAATACACATTACCAGTT+TGG + chr3_4:61645871-61645890 None:intergenic 30.0%
ACTTAACTAACCAAAAAGAC+AGG + chr3_4:61645903-61645922 None:intergenic 30.0%
TAATCTGTGTTCACTCAAAA+CGG - chr3_4:61645936-61645955 Msa0511940:five_prime_UTR 30.0%
ATCTAAAAATCCTCACATGG+TGG - chr3_4:61643084-61643103 Msa0511940:three_prime_UTR 35.0%
TGTTTAGTACCATGATAGGT+AGG - chr3_4:61643128-61643147 Msa0511940:three_prime_UTR 35.0%
GTTTAGTACCATGATAGGTA+GGG - chr3_4:61643129-61643148 Msa0511940:three_prime_UTR 35.0%
!!! CCTAGTGTTTTTTGTTCCAT+GGG - chr3_4:61643155-61643174 Msa0511940:three_prime_UTR 35.0%
GGATTTGAGCTCTATTTACA+AGG + chr3_4:61643464-61643483 None:intergenic 35.0%
TCAACTATGGCTATTACTGA+TGG - chr3_4:61643576-61643595 Msa0511940:intron 35.0%
! GGTAACAAATCTCTTGCTTT+TGG - chr3_4:61643604-61643623 Msa0511940:intron 35.0%
!! TTATGGTTCTGGATCTAAAG+TGG - chr3_4:61643627-61643646 Msa0511940:intron 35.0%
TATCGTAATACATGACCATG+TGG - chr3_4:61643651-61643670 Msa0511940:intron 35.0%
CAACTAAAACACATTCCACA+TGG + chr3_4:61643669-61643688 None:intergenic 35.0%
!! TTACCATTTCCTTTTGGTTG+GGG - chr3_4:61643839-61643858 Msa0511940:intron 35.0%
!!! GGTTTCTGGATTTTGATTAC+TGG + chr3_4:61644371-61644390 None:intergenic 35.0%
AACATCAACTAGACAGACAA+AGG - chr3_4:61644404-61644423 Msa0511940:CDS 35.0%
ACATCAACTAGACAGACAAA+GGG - chr3_4:61644405-61644424 Msa0511940:CDS 35.0%
! ACTTTTCACTAAGATGTGGT+GGG - chr3_4:61644456-61644475 Msa0511940:CDS 35.0%
TCTACGTAGAAAGGGATTTA+AGG - chr3_4:61644615-61644634 Msa0511940:CDS 35.0%
CTACGTAGAAAGGGATTTAA+GGG - chr3_4:61644616-61644635 Msa0511940:CDS 35.0%
! AGGAATCTTGAGAGTACAAT+TGG - chr3_4:61644909-61644928 Msa0511940:CDS 35.0%
CTAGGATTGTGAAAATTCTG+AGG - chr3_4:61645042-61645061 Msa0511940:five_prime_UTR 35.0%
! TGAAGCATCAGAGTTAATCA+TGG - chr3_4:61645165-61645184 Msa0511940:five_prime_UTR 35.0%
AACCATAAATATTCGGATGC+AGG - chr3_4:61645263-61645282 Msa0511940:five_prime_UTR 35.0%
ATAAATATTCGGATGCAGGT+GGG - chr3_4:61645267-61645286 Msa0511940:five_prime_UTR 35.0%
CATAAACAATGTCAGTGAAG+GGG + chr3_4:61645299-61645318 None:intergenic 35.0%
CCATAAACAATGTCAGTGAA+GGG + chr3_4:61645300-61645319 None:intergenic 35.0%
CTGACATTGTTTATGGCAAT+CGG - chr3_4:61645304-61645323 Msa0511940:five_prime_UTR 35.0%
ATTGTTTATGGCAATCGGAA+TGG - chr3_4:61645309-61645328 Msa0511940:five_prime_UTR 35.0%
TTGTTTATGGCAATCGGAAT+GGG - chr3_4:61645310-61645329 Msa0511940:five_prime_UTR 35.0%
! GGAATGGGTTTCTAACTATT+TGG - chr3_4:61645325-61645344 Msa0511940:five_prime_UTR 35.0%
GTTTCAATGATCGTGATGAT+AGG - chr3_4:61645639-61645658 Msa0511940:five_prime_UTR 35.0%
!! TAAAGTACTGGTCTAGAAGA+GGG - chr3_4:61645686-61645705 Msa0511940:five_prime_UTR 35.0%
CCCAGAAAATTAGTATGCTA+TGG + chr3_4:61645792-61645811 None:intergenic 35.0%
AACTAACCAAAAAGACAGGT+TGG + chr3_4:61645899-61645918 None:intergenic 35.0%
AATCTGTGTTCACTCAAAAC+GGG - chr3_4:61645937-61645956 Msa0511940:five_prime_UTR 35.0%
!!! TCGTTTTTTTCCACCATGTG+AGG + chr3_4:61643097-61643116 None:intergenic 40.0%
!!! GCCTAGTGTTTTTTGTTCCA+TGG - chr3_4:61643154-61643173 Msa0511940:three_prime_UTR 40.0%
CCCATGGAACAAAAAACACT+AGG + chr3_4:61643158-61643177 None:intergenic 40.0%
!!! TTTTTTGTTCCATGGGTTGC+AGG - chr3_4:61643162-61643181 Msa0511940:three_prime_UTR 40.0%
CAGGATAGTGTCTACTCATT+TGG - chr3_4:61643181-61643200 Msa0511940:three_prime_UTR 40.0%
TTACGCAGGTGATTGATACA+AGG - chr3_4:61643340-61643359 Msa0511940:CDS 40.0%
TACGCAGGTGATTGATACAA+GGG - chr3_4:61643341-61643360 Msa0511940:CDS 40.0%
TTGAGTCATGATTTCACGTG+TGG - chr3_4:61643386-61643405 Msa0511940:CDS 40.0%
!! CTTGCTTTTGGTTATGGTTC+TGG - chr3_4:61643616-61643635 Msa0511940:intron 40.0%
AGTAGACACAACACTAAGCA+AGG - chr3_4:61643770-61643789 Msa0511940:CDS 40.0%
ATACCCCAACCAAAAGGAAA+TGG + chr3_4:61643845-61643864 None:intergenic 40.0%
ACATGTATACCCCAACCAAA+AGG + chr3_4:61643851-61643870 None:intergenic 40.0%
! GGTGAGTTTTCTCTTTCGTT+GGG + chr3_4:61644158-61644177 None:intergenic 40.0%
! TGGTGAGTTTTCTCTTTCGT+TGG + chr3_4:61644159-61644178 None:intergenic 40.0%
GACCATAGAAGTTTCTCAGA+GGG + chr3_4:61644213-61644232 None:intergenic 40.0%
AGACACAGAGTTGCAACAAA+GGG - chr3_4:61644245-61644264 Msa0511940:intron 40.0%
AAGGGCTAGAAAGCAATTAC+AGG - chr3_4:61644263-61644282 Msa0511940:intron 40.0%
! TTGATGTTGACTGTGGTTTC+TGG + chr3_4:61644392-61644411 None:intergenic 40.0%
! TGTCTAGTTGATGTTGACTG+TGG + chr3_4:61644399-61644418 None:intergenic 40.0%
AGACAGACAAAGGGAATTCA+AGG - chr3_4:61644414-61644433 Msa0511940:CDS 40.0%
! GTGCACTTTTCACTAAGATG+TGG - chr3_4:61644452-61644471 Msa0511940:CDS 40.0%
! CACTTTTCACTAAGATGTGG+TGG - chr3_4:61644455-61644474 Msa0511940:CDS 40.0%
TGGCCAAAGAATCAACTTTG+AGG - chr3_4:61644498-61644517 Msa0511940:CDS 40.0%
GGCCAAAGAATCAACTTTGA+GGG - chr3_4:61644499-61644518 Msa0511940:CDS 40.0%
! TGCCCTCAAAGTTGATTCTT+TGG + chr3_4:61644504-61644523 None:intergenic 40.0%
GGGCATTCTATGTTCAACTA+TGG - chr3_4:61644519-61644538 Msa0511940:CDS 40.0%
! TATGGGGCAGTGACAATTTT+GGG + chr3_4:61644558-61644577 None:intergenic 40.0%
! ATATGGGGCAGTGACAATTT+TGG + chr3_4:61644559-61644578 None:intergenic 40.0%
CTTTCCAATGAAGACTCAAG+TGG + chr3_4:61644700-61644719 None:intergenic 40.0%
GCTGTATTTAGTAACTCAGC+TGG - chr3_4:61644748-61644767 Msa0511940:CDS 40.0%
CAGCTGGTAATTCTAACATG+AGG - chr3_4:61644764-61644783 Msa0511940:CDS 40.0%
!!! TGAGTGGTGCTTTTTCTGTA+TGG - chr3_4:61644953-61644972 Msa0511940:CDS 40.0%
CACCTGCATCCGAATATTTA+TGG + chr3_4:61645268-61645287 None:intergenic 40.0%
CATAAATATTCGGATGCAGG+TGG - chr3_4:61645266-61645285 Msa0511940:five_prime_UTR 40.0%
CCCTTCACTGACATTGTTTA+TGG - chr3_4:61645297-61645316 Msa0511940:five_prime_UTR 40.0%
GCCATAAACAATGTCAGTGA+AGG + chr3_4:61645301-61645320 None:intergenic 40.0%
GGAGCCATTTATTGTTAAGC+AGG - chr3_4:61645371-61645390 Msa0511940:five_prime_UTR 40.0%
AATGATCGTGATGATAGGTG+AGG - chr3_4:61645644-61645663 Msa0511940:five_prime_UTR 40.0%
!! GTAAAGTACTGGTCTAGAAG+AGG - chr3_4:61645685-61645704 Msa0511940:five_prime_UTR 40.0%
! TTGACACAATGTCTAGGATC+TGG + chr3_4:61645739-61645758 None:intergenic 40.0%
AGACAAGTCTATCAAGGATG+TGG + chr3_4:61645769-61645788 None:intergenic 40.0%
CTATGGAGACAAGTCTATCA+AGG + chr3_4:61645775-61645794 None:intergenic 40.0%
!! AACCAAAAAGACAGGTTGGT+GGG + chr3_4:61645895-61645914 None:intergenic 40.0%
! TAACCAAAAAGACAGGTTGG+TGG + chr3_4:61645896-61645915 None:intergenic 40.0%
ACAAGGGTAAGTTACCTGAC+AGG - chr3_4:61643357-61643376 Msa0511940:CDS 45.0%
TGACTCAACACTAACCTGTC+AGG + chr3_4:61643374-61643393 None:intergenic 45.0%
TGGCTATTACTGATGGCAAG+TGG - chr3_4:61643583-61643602 Msa0511940:intron 45.0%
CATACAGCTGTAGCAACATG+TGG - chr3_4:61644032-61644051 Msa0511940:intron 45.0%
! CATGATGATGTCTGGGAATG+TGG + chr3_4:61644135-61644154 None:intergenic 45.0%
! TGCATTGGCAAGTGATAGAG+TGG + chr3_4:61644179-61644198 None:intergenic 45.0%
AGAAGTTTCTCAGAGGGACT+TGG + chr3_4:61644207-61644226 None:intergenic 45.0%
! GTCCCTCTGAGAAACTTCTA+TGG - chr3_4:61644208-61644227 Msa0511940:intron 45.0%
GGACCATAGAAGTTTCTCAG+AGG + chr3_4:61644214-61644233 None:intergenic 45.0%
TTGTTGCAACTCTGTGTCTG+AGG + chr3_4:61644244-61644263 None:intergenic 45.0%
CAGACACAGAGTTGCAACAA+AGG - chr3_4:61644244-61644263 Msa0511940:intron 45.0%
!! TGACTGTGGTTTCTGGTTTC+TGG + chr3_4:61644385-61644404 None:intergenic 45.0%
TACCCTACTCTTTAACGCTC+TGG - chr3_4:61644671-61644690 Msa0511940:CDS 45.0%
TCCAATGAAGACTCAAGTGG+AGG + chr3_4:61644697-61644716 None:intergenic 45.0%
CTTCGTGAGTATGACCATGA+TGG - chr3_4:61644876-61644895 Msa0511940:CDS 45.0%
ACCATGATGGTTCAAAAGCG+AGG - chr3_4:61644889-61644908 Msa0511940:CDS 45.0%
!! TCCTCGCTTTTGAACCATCA+TGG + chr3_4:61644893-61644912 None:intergenic 45.0%
AAGTCATCAGACACAGTGAG+TGG - chr3_4:61644937-61644956 Msa0511940:CDS 45.0%
TAGGATCTGGCAATAGCTTC+TGG + chr3_4:61645726-61645745 None:intergenic 45.0%
GGCTCTTTGACACAATGTCT+AGG + chr3_4:61645745-61645764 None:intergenic 45.0%
CAAGTCTATCAAGGATGTGG+CGG + chr3_4:61645766-61645785 None:intergenic 45.0%
CTAGGCAACCCTACCTATCA+TGG + chr3_4:61643140-61643159 None:intergenic 50.0%
AGACACTATCCTGCAACCCA+TGG + chr3_4:61643174-61643193 None:intergenic 50.0%
AGCTGTAGCAACATGTGGTC+AGG - chr3_4:61644037-61644056 Msa0511940:intron 50.0%
GGAATGTGGTACGAGCAACT+GGG + chr3_4:61644121-61644140 None:intergenic 50.0%
! CGTTGGGCATGATGATGTCT+GGG + chr3_4:61644142-61644161 None:intergenic 50.0%
! TCGTTGGGCATGATGATGTC+TGG + chr3_4:61644143-61644162 None:intergenic 50.0%
AGGGACTTGGCTATCTGCAT+TGG + chr3_4:61644194-61644213 None:intergenic 50.0%
CTCTGTGTCTGAGGAAGACT+TGG + chr3_4:61644235-61644254 None:intergenic 50.0%
GCAGATGTGAAAGCGGCATT+TGG - chr3_4:61644478-61644497 Msa0511940:CDS 50.0%
TGGGCTGCTCTACGTAGAAA+GGG - chr3_4:61644607-61644626 Msa0511940:CDS 50.0%
! GGCCAGAGCGTTAAAGAGTA+GGG + chr3_4:61644676-61644695 None:intergenic 50.0%
! AGGCCAGAGCGTTAAAGAGT+AGG + chr3_4:61644677-61644696 None:intergenic 50.0%
GCCTCCACTTGAGTCTTCAT+TGG - chr3_4:61644693-61644712 Msa0511940:CDS 50.0%
ATTTCTTCAGCTGTGCCACC+TGG + chr3_4:61645127-61645146 None:intergenic 50.0%
AGAGCCATTGAGTCTTGCAG+AGG - chr3_4:61645350-61645369 Msa0511940:five_prime_UTR 50.0%
GGCAATAGCTTCTGGTCAAG+TGG + chr3_4:61645718-61645737 None:intergenic 50.0%
! CACCCACCAACCTGTCTTTT+TGG - chr3_4:61645890-61645909 Msa0511940:five_prime_UTR 50.0%
GGGAATGTGGTACGAGCAAC+TGG + chr3_4:61644122-61644141 None:intergenic 55.0%
!! GTGGTGGGCAGATGTGAAAG+CGG - chr3_4:61644471-61644490 Msa0511940:CDS 55.0%
CTGGGCTGCTCTACGTAGAA+AGG - chr3_4:61644606-61644625 Msa0511940:CDS 55.0%
ACTGCAGGAGTGAAGAAGCC+AGG - chr3_4:61645106-61645125 Msa0511940:five_prime_UTR 55.0%
GGCTCCTCTGCAAGACTCAA+TGG + chr3_4:61645357-61645376 None:intergenic 55.0%
GCAGGAGTGAAGAAGCCAGG+TGG - chr3_4:61645109-61645128 Msa0511940:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 61643061 61646027 61643061 ID=Msa0511940;Name=Msa0511940
chr3_4 mRNA 61643061 61646027 61643061 ID=Msa0511940-mRNA-1;Parent=Msa0511940;Name=Msa0511940-mRNA-1;_AED=0.02;_eAED=0.02;_QI=1027|1|1|1|1|1|5|250|352
chr3_4 exon 61643061 61643427 61643061 ID=Msa0511940-mRNA-1:exon:15092;Parent=Msa0511940-mRNA-1
chr3_4 exon 61643696 61643803 61643696 ID=Msa0511940-mRNA-1:exon:15091;Parent=Msa0511940-mRNA-1
chr3_4 exon 61643902 61643975 61643902 ID=Msa0511940-mRNA-1:exon:15090;Parent=Msa0511940-mRNA-1
chr3_4 exon 61644137 61644214 61644137 ID=Msa0511940-mRNA-1:exon:15089;Parent=Msa0511940-mRNA-1
chr3_4 exon 61644319 61646027 61644319 ID=Msa0511940-mRNA-1:exon:15088;Parent=Msa0511940-mRNA-1
chr3_4 five_prime_UTR 61645001 61646027 61645001 ID=Msa0511940-mRNA-1:five_prime_utr;Parent=Msa0511940-mRNA-1
chr3_4 CDS 61644319 61645000 61644319 ID=Msa0511940-mRNA-1:cds;Parent=Msa0511940-mRNA-1
chr3_4 CDS 61644137 61644214 61644137 ID=Msa0511940-mRNA-1:cds;Parent=Msa0511940-mRNA-1
chr3_4 CDS 61643902 61643975 61643902 ID=Msa0511940-mRNA-1:cds;Parent=Msa0511940-mRNA-1
chr3_4 CDS 61643696 61643803 61643696 ID=Msa0511940-mRNA-1:cds;Parent=Msa0511940-mRNA-1
chr3_4 CDS 61643311 61643427 61643311 ID=Msa0511940-mRNA-1:cds;Parent=Msa0511940-mRNA-1
chr3_4 three_prime_UTR 61643061 61643310 61643061 ID=Msa0511940-mRNA-1:three_prime_utr;Parent=Msa0511940-mRNA-1
Gene Sequence

>Msa0511940

ATGCTGTCTACTAGTGCTGCTGCTCAGCTTCCCAGTTGCTCGTACCACATTCCCAGACATCATCATGCCCAACGAAAGAGAAAACTCACCACTCTATCACTTGCCAATGCAGATAGCCAAGTCCCTCTGAGAAACTTCTATGGTCCAAGTCTTCCTCAGACACAGAGTTGCAACAAAGGGCTAGAAAGCAATTACAGGTCTAGATACAATTCCAATTCAAATCAGAACGACAAACACCTATTCCTTATTAAATACTTCTCACTTGCTGACTCAGAAGACACCAGTAATCAAAATCCAGAAACCAGAAACCACAGTCAACATCAACTAGACAGACAAAGGGAATTCAAGGAACACAAAAAAGACAGTGCACTTTTCACTAAGATGTGGTGGGCAGATGTGAAAGCGGCATTTGGCCAAAGAATCAACTTTGAGGGCATTCTATGTTCAACTATGGTCATCTTAAAAGACCCAAAATTGTCACTGCCCCATATTTCAGTTCCTGATATAAGATACATTGACTGGGCTGCTCTACGTAGAAAGGGATTTAAGGGTGTTGTCTTTGACAAAGATAATACTATTACAGTACCCTACTCTTTAACGCTCTGGCCTCCACTTGAGTCTTCATTGGAAAGTTGTAAATCAGAGTTTGGTCAAGATATTGCTGTATTTAGTAACTCAGCTGGACTTCGTGAGTATGACCATGATGGTTCAAAAGCGAGGAATCTTGAGAGTACAATTGGAATTAAAGTCATCAGACACAGAGTGAAGAAGCCAGGTGGCACAGCTGAAGAAATTGAAAAGCATTTTGGCTGTGAAGCATCAGAGTTAATCATGGTGGGTGATCGCCCCTTCACTGACATTGTTTATGGCAATCGGAATGGGTTTCTAACTATTTGGACAGAGCCATTGAGTCTTGCAGAGGAGCCATTTATTGTTAAGCAGGTGAGGAAGCTAGAAACTACATTTGTAAAGTACTGGTCTAGAAGAGGGTTGAAGCCACTTGACCAGAAGCTATTGCCAGATCCTAGACATTGTGTCAAAGAGCCGCCACATCCTTGA
Protein sequence

>Msa0511940

MLSTSAAAQLPSCSYHIPRHHHAQRKRKLTTLSLANADSQVPLRNFYGPSLPQTQSCNKGLESNYRSRYNSNSNQNDKHLFLIKYFSLADSEDTSNQNPETRNHSQHQLDRQREFKEHKKDSALFTKMWWADVKAAFGQRINFEGILCSTMVILKDPKLSLPHISVPDIRYIDWAALRRKGFKGVVFDKDNTITVPYSLTLWPPLESSLESCKSEFGQDIAVFSNSAGLREYDHDGSKARNLESTIGIKVIRHRVKKPGGTAEEIEKHFGCEASELIMVGDRPFTDIVYGNRNGFLTIWTEPLSLAEEPFIVKQVRKLETTFVKYWSRRGLKPLDQKLLPDPRHCVKEPPHP*