AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0437110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0437110 MtrunA17_Chr7g0263221 70.400 250 13 3 2 251 48 236 3.55e-112 322
Msa0437110 MtrunA17_Chr1g0181861 58.800 250 42 3 2 251 49 237 4.05e-94 276
Msa0437110 MtrunA17_Chr1g0213361 45.565 248 73 4 2 249 130 315 1.07e-61 196
Msa0437110 MtrunA17_Chr1g0181851 46.559 247 38 4 2 248 45 197 1.26e-59 188
Msa0437110 MtrunA17_Chr1g0181841 77.778 72 16 0 2 73 70 141 2.76e-35 123
Msa0437110 MtrunA17_Chr3g0107731 30.055 183 101 7 32 204 59 224 1.47e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0437110 AT5G02790.1 50.607 247 61 3 2 248 50 235 4.05e-77 233
Msa0437110 AT5G02780.1 47.154 246 68 4 1 245 51 235 1.85e-68 211
Msa0437110 AT5G02780.3 47.154 246 68 4 1 245 51 235 1.85e-68 211
Msa0437110 AT5G02780.2 47.154 246 66 5 1 245 51 233 2.01e-66 206
Msa0437110 AT3G55040.1 43.373 249 80 3 2 250 102 289 1.73e-62 198

Find 69 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACACTAATGATACTTTAT+TGG 0.193930 3_2:+91100949 None:intergenic
GCAGGCTCAACAGTGAAAAT+TGG 0.195050 3_2:-91101006 Msa0437110:intron
GGTGAAATATCTATAATAAT+TGG 0.205562 3_2:-91100733 None:intergenic
AGACAGGCCTGTTTGGTATA+AGG 0.282787 3_2:-91102344 Msa0437110:CDS
TCACCAAACTCTTTCTTTGC+AGG 0.295908 3_2:+91102040 None:intergenic
TCCGTTTCCATTTGCGGAAC+AGG 0.303576 3_2:+91101321 None:intergenic
CCCATTTCCGTTTCCATTTG+CGG 0.305037 3_2:+91101315 None:intergenic
CAATGAAGAGTACAGATCTT+TGG 0.308227 3_2:+91101988 None:intergenic
CGGAAATGGGCCACAGAAAA+TGG 0.312516 3_2:-91101302 Msa0437110:intron
AAAATATCAACAATTTCATT+TGG 0.321951 3_2:+91101151 None:intergenic
ACTACAAGACAAAATCAAAT+TGG 0.371760 3_2:-91102383 Msa0437110:CDS
AGGTCCTGCCTTTGATTACT+TGG 0.371880 3_2:-91101855 Msa0437110:CDS
ACACCTCAGAAAAGACTATA+TGG 0.374447 3_2:+91101500 None:intergenic
TCAATCCATGCTGCAAGTTT+CGG 0.375231 3_2:+91101451 None:intergenic
TCCGCAAATGGAAACGGAAA+TGG 0.375924 3_2:-91101316 Msa0437110:CDS
TCTTTCAACAAATGGAATAT+AGG 0.379083 3_2:+91101525 None:intergenic
ACCTTCAAGACAGGCCTGTT+TGG 0.380904 3_2:-91102351 Msa0437110:CDS
GGCCTGTCTTGAAGGTTAAT+AGG 0.408791 3_2:+91102358 None:intergenic
ATATGGAATCTTTCAACAAA+TGG 0.410892 3_2:+91101517 None:intergenic
GTGAAATATCTATAATAATT+GGG 0.411290 3_2:-91100732 None:intergenic
ACCAAACAGGCCTGTCTTGA+AGG 0.413697 3_2:+91102350 None:intergenic
AACTTAACCTGTTCCGCAAA+TGG 0.414010 3_2:-91101328 Msa0437110:CDS
TATGAATTGATTACAAATCA+AGG 0.418475 3_2:-91101373 Msa0437110:CDS
CAGGCTCAACAGTGAAAATT+GGG 0.419928 3_2:-91101005 Msa0437110:exon
TTCAAGCATGATATAATAGA+AGG 0.420899 3_2:-91101478 Msa0437110:CDS
GATCCTGCAAAGAAAGAGTT+TGG 0.421571 3_2:-91102043 Msa0437110:CDS
GGAGCACAATGGGAAGGTCT+TGG 0.435080 3_2:-91102208 Msa0437110:CDS
ACTTTCTCCTTATACCAAAC+AGG 0.438140 3_2:+91102337 None:intergenic
ATCAACAATTTCATTTGGAT+CGG 0.455372 3_2:+91101156 None:intergenic
TTCATAAACGAATGCAATTG+TGG 0.455775 3_2:+91101390 None:intergenic
GATTACTTGGAGAATGCTCT+TGG 0.461042 3_2:-91101842 Msa0437110:CDS
GAGCACAATGGGAAGGTCTT+GGG 0.461051 3_2:-91102207 Msa0437110:CDS
TGCCATCGTTGGAGCACAAT+GGG 0.465276 3_2:-91102218 Msa0437110:CDS
TACATAGATGTCAACTTTGA+GGG 0.472144 3_2:-91102162 Msa0437110:CDS
TTGATGATGGACAATTTCCT+AGG 0.478787 3_2:-91101814 Msa0437110:CDS
TTACATAGATGTCAACTTTG+AGG 0.479491 3_2:-91102163 Msa0437110:CDS
ACCTGTTCCGCAAATGGAAA+CGG 0.480344 3_2:-91101322 Msa0437110:CDS
CCGCAAATGGAAACGGAAAT+GGG 0.488839 3_2:-91101315 Msa0437110:CDS
TTGTCTTGTAGTCCCTGCAT+TGG 0.490449 3_2:+91102394 None:intergenic
GCTCTTGGTAAATTTGATGA+TGG 0.492656 3_2:-91101827 Msa0437110:CDS
TTACCCAACTGAATTGTCCT+AGG 0.504543 3_2:+91101797 None:intergenic
ATTTCCTAGGACAATTCAGT+TGG 0.506498 3_2:-91101801 Msa0437110:CDS
TTCCTATTAACCTTCAAGAC+AGG 0.513215 3_2:-91102360 Msa0437110:CDS
TCATAAACGAATGCAATTGT+GGG 0.515423 3_2:+91101391 None:intergenic
GATCTGTACTCTTCATTGAA+AGG 0.526123 3_2:-91101983 Msa0437110:CDS
CAGTGAAAATTGGGTTGCAA+TGG 0.530254 3_2:-91100996 Msa0437110:exon
TTTCCTAGGACAATTCAGTT+GGG 0.539300 3_2:-91101800 Msa0437110:CDS
TTCTCCAAGTAATCAAAGGC+AGG 0.548201 3_2:+91101851 None:intergenic
GGCAGCAGTCTACCTCTCAA+AGG 0.554898 3_2:+91100970 None:intergenic
GCAGCAGTCTACCTCTCAAA+GGG 0.555531 3_2:+91100971 None:intergenic
TTCCCATTGTGCTCCAACGA+TGG 0.562767 3_2:+91102216 None:intergenic
CATGATTGATGACCAATGCA+GGG 0.569230 3_2:-91102406 None:intergenic
GGAGATCCTATAAAACAAGC+TGG 0.576421 3_2:-91101962 Msa0437110:intron
CTCACACTGGGAACTAGAGA+TGG 0.581337 3_2:+91102138 None:intergenic
GAAACTTGCAGCATGGATTG+AGG 0.586391 3_2:-91101449 Msa0437110:CDS
AGCATTCTCCAAGTAATCAA+AGG 0.590018 3_2:+91101847 None:intergenic
AGGTCATCAATCCGAACTTG+AGG 0.592258 3_2:-91101353 Msa0437110:CDS
TTGCAATGGCACCCTTTGAG+AGG 0.592579 3_2:-91100982 Msa0437110:three_prime_UTR
GAAAGTCTACCCAGAAAACA+AGG 0.594976 3_2:-91102320 Msa0437110:intron
GTATGTATAGGTGCCATCGT+TGG 0.598903 3_2:-91102229 Msa0437110:intron
GTGCCATCGTTGGAGCACAA+TGG 0.601381 3_2:-91102219 Msa0437110:CDS
AGCATGATATAATAGAAGGA+AGG 0.603963 3_2:-91101474 Msa0437110:CDS
ATCGTTGGAGCACAATGGGA+AGG 0.614660 3_2:-91102214 Msa0437110:CDS
ACATAGATGTCAACTTTGAG+GGG 0.620984 3_2:-91102161 Msa0437110:CDS
GAAGGCCGAAACTTGCAGCA+TGG 0.634072 3_2:-91101456 Msa0437110:CDS
TATTTCACCATCCATCAACA+TGG 0.644962 3_2:+91100747 None:intergenic
ACATTATTTATACAATACAG+TGG 0.670741 3_2:+91100793 None:intergenic
TTGATGCTTATACACAGACA+AGG 0.678414 3_2:-91101180 Msa0437110:CDS
TGATGCTTATACACAGACAA+GGG 0.702603 3_2:-91101179 Msa0437110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATAATCGATTTTTTTT+GGG + chr3_2:91101031-91101050 None:intergenic 10.0%
!!! TAATATAATCGATTTTTTTT+GGG + chr3_2:91101031-91101050 None:intergenic 10.0%
!!! ATTATTTTTTTCGTATGTAT+AGG - chr3_2:91100884-91100903 Msa0437110:three_prime_UTR 15.0%
!!! AATATAATCGATTTTTTTTG+GGG + chr3_2:91101030-91101049 None:intergenic 15.0%
!!! CTAATATAATCGATTTTTTT+TGG + chr3_2:91101032-91101051 None:intergenic 15.0%
!! AAAAAAATCGATTATATTAG+TGG - chr3_2:91101032-91101051 Msa0437110:intron 15.0%
!!! ATTTTATGATTTTGTTTTTC+AGG - chr3_2:91101060-91101079 Msa0437110:intron 15.0%
!! TCTTATATATAAGTTGTTAT+TGG - chr3_2:91101554-91101573 Msa0437110:intron 15.0%
!! AAAATATCAACAATTTCATT+TGG + chr3_2:91101977-91101996 None:intergenic 15.0%
!!! ATTATTTTTTTCGTATGTAT+AGG - chr3_2:91100884-91100903 Msa0437110:three_prime_UTR 15.0%
!!! AATATAATCGATTTTTTTTG+GGG + chr3_2:91101030-91101049 None:intergenic 15.0%
!!! CTAATATAATCGATTTTTTT+TGG + chr3_2:91101032-91101051 None:intergenic 15.0%
!! AAAAAAATCGATTATATTAG+TGG - chr3_2:91101032-91101051 Msa0437110:intron 15.0%
!!! ATTTTATGATTTTGTTTTTC+AGG - chr3_2:91101060-91101079 Msa0437110:intron 15.0%
!! TCTTATATATAAGTTGTTAT+TGG - chr3_2:91101554-91101573 Msa0437110:intron 15.0%
!! AAAATATCAACAATTTCATT+TGG + chr3_2:91101977-91101996 None:intergenic 15.0%
!! GAAAAAAATAATGATCAACT+AGG + chr3_2:91100876-91100895 None:intergenic 20.0%
!!! ATATAATCGATTTTTTTTGG+GGG + chr3_2:91101029-91101048 None:intergenic 20.0%
!!! ATTTTAAGCATATTACTTGT+CGG - chr3_2:91101394-91101413 Msa0437110:CDS 20.0%
!!! ATTTTTAATAAAGCATGTGA+TGG - chr3_2:91101437-91101456 Msa0437110:CDS 20.0%
!! TATGAATTGATTACAAATCA+AGG - chr3_2:91101752-91101771 Msa0437110:intron 20.0%
!! AAACACTAATGATACTTTAT+TGG + chr3_2:91102179-91102198 None:intergenic 20.0%
!! ACATTATTTATACAATACAG+TGG + chr3_2:91102335-91102354 None:intergenic 20.0%
!! GAAAAAAATAATGATCAACT+AGG + chr3_2:91100876-91100895 None:intergenic 20.0%
!!! ATATAATCGATTTTTTTTGG+GGG + chr3_2:91101029-91101048 None:intergenic 20.0%
!!! ATTTTAAGCATATTACTTGT+CGG - chr3_2:91101394-91101413 Msa0437110:CDS 20.0%
!!! ATTTTTAATAAAGCATGTGA+TGG - chr3_2:91101437-91101456 Msa0437110:CDS 20.0%
!! TATGAATTGATTACAAATCA+AGG - chr3_2:91101752-91101771 Msa0437110:intron 20.0%
!! AAACACTAATGATACTTTAT+TGG + chr3_2:91102179-91102198 None:intergenic 20.0%
!! ACATTATTTATACAATACAG+TGG + chr3_2:91102335-91102354 None:intergenic 20.0%
! ACTACAAGACAAAATCAAAT+TGG - chr3_2:91100742-91100761 Msa0437110:three_prime_UTR 25.0%
!!! GCTTTCAAGTTAATAAATAG+AGG - chr3_2:91101190-91101209 Msa0437110:CDS 25.0%
!! TGAAATTGGTAATTTGAATC+AGG - chr3_2:91101250-91101269 Msa0437110:intron 25.0%
! TAAGAGAAAAACTATAGTCA+TGG + chr3_2:91101539-91101558 None:intergenic 25.0%
! TCTTTCAACAAATGGAATAT+AGG + chr3_2:91101603-91101622 None:intergenic 25.0%
! ATATGGAATCTTTCAACAAA+TGG + chr3_2:91101611-91101630 None:intergenic 25.0%
! TTCAAGCATGATATAATAGA+AGG - chr3_2:91101647-91101666 Msa0437110:intron 25.0%
! ATCAACAATTTCATTTGGAT+CGG + chr3_2:91101972-91101991 None:intergenic 25.0%
! AACATTGTGAGTTTAAAAAG+TGG + chr3_2:91102092-91102111 None:intergenic 25.0%
! ACTACAAGACAAAATCAAAT+TGG - chr3_2:91100742-91100761 Msa0437110:three_prime_UTR 25.0%
!!! GCTTTCAAGTTAATAAATAG+AGG - chr3_2:91101190-91101209 Msa0437110:CDS 25.0%
!! TGAAATTGGTAATTTGAATC+AGG - chr3_2:91101250-91101269 Msa0437110:intron 25.0%
! TAAGAGAAAAACTATAGTCA+TGG + chr3_2:91101539-91101558 None:intergenic 25.0%
! TCTTTCAACAAATGGAATAT+AGG + chr3_2:91101603-91101622 None:intergenic 25.0%
! ATATGGAATCTTTCAACAAA+TGG + chr3_2:91101611-91101630 None:intergenic 25.0%
! TTCAAGCATGATATAATAGA+AGG - chr3_2:91101647-91101666 Msa0437110:intron 25.0%
! ATCAACAATTTCATTTGGAT+CGG + chr3_2:91101972-91101991 None:intergenic 25.0%
! AACATTGTGAGTTTAAAAAG+TGG + chr3_2:91102092-91102111 None:intergenic 25.0%
!!! TTAAACACACCTTGTTTTCT+GGG + chr3_2:91100817-91100836 None:intergenic 30.0%
TTACATAGATGTCAACTTTG+AGG - chr3_2:91100962-91100981 Msa0437110:three_prime_UTR 30.0%
TACATAGATGTCAACTTTGA+GGG - chr3_2:91100963-91100982 Msa0437110:three_prime_UTR 30.0%
!!! GCAAACTGAACTTTTGAAAT+TGG - chr3_2:91101236-91101255 Msa0437110:intron 30.0%
!!! TTGCCATTTTTCTTTTCTGT+TGG - chr3_2:91101367-91101386 Msa0437110:CDS 30.0%
CAACCAACAGAAAAGAAAAA+TGG + chr3_2:91101373-91101392 None:intergenic 30.0%
!! TGTTATTGGTTGTGTTGATT+AGG - chr3_2:91101568-91101587 Msa0437110:intron 30.0%
! ATTCCATATAGTCTTTTCTG+AGG - chr3_2:91101622-91101641 Msa0437110:intron 30.0%
AGCATGATATAATAGAAGGA+AGG - chr3_2:91101651-91101670 Msa0437110:intron 30.0%
TCATAAACGAATGCAATTGT+GGG + chr3_2:91101737-91101756 None:intergenic 30.0%
TTCATAAACGAATGCAATTG+TGG + chr3_2:91101738-91101757 None:intergenic 30.0%
!! TATTTTCAAGAAACGCTTTC+TGG - chr3_2:91101991-91102010 Msa0437110:CDS 30.0%
TAAAAAGTGGCGAATACAAT+AGG + chr3_2:91102079-91102098 None:intergenic 30.0%
!!! TTAAACACACCTTGTTTTCT+GGG + chr3_2:91100817-91100836 None:intergenic 30.0%
TTACATAGATGTCAACTTTG+AGG - chr3_2:91100962-91100981 Msa0437110:three_prime_UTR 30.0%
TACATAGATGTCAACTTTGA+GGG - chr3_2:91100963-91100982 Msa0437110:three_prime_UTR 30.0%
!!! GCAAACTGAACTTTTGAAAT+TGG - chr3_2:91101236-91101255 Msa0437110:intron 30.0%
!!! TTGCCATTTTTCTTTTCTGT+TGG - chr3_2:91101367-91101386 Msa0437110:CDS 30.0%
CAACCAACAGAAAAGAAAAA+TGG + chr3_2:91101373-91101392 None:intergenic 30.0%
!! TGTTATTGGTTGTGTTGATT+AGG - chr3_2:91101568-91101587 Msa0437110:intron 30.0%
! ATTCCATATAGTCTTTTCTG+AGG - chr3_2:91101622-91101641 Msa0437110:intron 30.0%
AGCATGATATAATAGAAGGA+AGG - chr3_2:91101651-91101670 Msa0437110:intron 30.0%
TCATAAACGAATGCAATTGT+GGG + chr3_2:91101737-91101756 None:intergenic 30.0%
TTCATAAACGAATGCAATTG+TGG + chr3_2:91101738-91101757 None:intergenic 30.0%
!! TATTTTCAAGAAACGCTTTC+TGG - chr3_2:91101991-91102010 Msa0437110:CDS 30.0%
TAAAAAGTGGCGAATACAAT+AGG + chr3_2:91102079-91102098 None:intergenic 30.0%
TTCCTATTAACCTTCAAGAC+AGG - chr3_2:91100765-91100784 Msa0437110:three_prime_UTR 35.0%
ACTTTCTCCTTATACCAAAC+AGG + chr3_2:91100791-91100810 None:intergenic 35.0%
!!! GTTAAACACACCTTGTTTTC+TGG + chr3_2:91100818-91100837 None:intergenic 35.0%
!! TGTGTTTAACTTGCTCATAC+AGG - chr3_2:91100828-91100847 Msa0437110:three_prime_UTR 35.0%
!! GTGTTTAACTTGCTCATACA+GGG - chr3_2:91100829-91100848 Msa0437110:three_prime_UTR 35.0%
ACATAGATGTCAACTTTGAG+GGG - chr3_2:91100964-91100983 Msa0437110:three_prime_UTR 35.0%
! CAATGAAGAGTACAGATCTT+TGG + chr3_2:91101140-91101159 None:intergenic 35.0%
GATCTGTACTCTTCATTGAA+AGG - chr3_2:91101142-91101161 Msa0437110:CDS 35.0%
!!! GCATTACCAGCTTGTTTTAT+AGG + chr3_2:91101172-91101191 None:intergenic 35.0%
AGCATTCTCCAAGTAATCAA+AGG + chr3_2:91101281-91101300 None:intergenic 35.0%
GCTCTTGGTAAATTTGATGA+TGG - chr3_2:91101298-91101317 Msa0437110:intron 35.0%
!! TTGATGATGGACAATTTCCT+AGG - chr3_2:91101311-91101330 Msa0437110:CDS 35.0%
ATTTCCTAGGACAATTCAGT+TGG - chr3_2:91101324-91101343 Msa0437110:CDS 35.0%
TTTCCTAGGACAATTCAGTT+GGG - chr3_2:91101325-91101344 Msa0437110:CDS 35.0%
!! TATTGGTTGTGTTGATTAGG+TGG - chr3_2:91101571-91101590 Msa0437110:intron 35.0%
ACACCTCAGAAAAGACTATA+TGG + chr3_2:91101628-91101647 None:intergenic 35.0%
! GGTTAAGTTTTCCTCAAGTT+CGG + chr3_2:91101786-91101805 None:intergenic 35.0%
GTGCATAATGTTTCTTTGAC+AGG - chr3_2:91101910-91101929 Msa0437110:intron 35.0%
TTGATGCTTATACACAGACA+AGG - chr3_2:91101945-91101964 Msa0437110:intron 35.0%
TGATGCTTATACACAGACAA+GGG - chr3_2:91101946-91101965 Msa0437110:intron 35.0%
! ATGATGCTTTTCCATGTTGA+TGG - chr3_2:91102367-91102386 Msa0437110:CDS 35.0%
TATTTCACCATCCATCAACA+TGG + chr3_2:91102381-91102400 None:intergenic 35.0%
TTCCTATTAACCTTCAAGAC+AGG - chr3_2:91100765-91100784 Msa0437110:three_prime_UTR 35.0%
ACTTTCTCCTTATACCAAAC+AGG + chr3_2:91100791-91100810 None:intergenic 35.0%
!!! GTTAAACACACCTTGTTTTC+TGG + chr3_2:91100818-91100837 None:intergenic 35.0%
!! TGTGTTTAACTTGCTCATAC+AGG - chr3_2:91100828-91100847 Msa0437110:three_prime_UTR 35.0%
!! GTGTTTAACTTGCTCATACA+GGG - chr3_2:91100829-91100848 Msa0437110:three_prime_UTR 35.0%
ACATAGATGTCAACTTTGAG+GGG - chr3_2:91100964-91100983 Msa0437110:three_prime_UTR 35.0%
! CAATGAAGAGTACAGATCTT+TGG + chr3_2:91101140-91101159 None:intergenic 35.0%
GATCTGTACTCTTCATTGAA+AGG - chr3_2:91101142-91101161 Msa0437110:CDS 35.0%
!!! GCATTACCAGCTTGTTTTAT+AGG + chr3_2:91101172-91101191 None:intergenic 35.0%
AGCATTCTCCAAGTAATCAA+AGG + chr3_2:91101281-91101300 None:intergenic 35.0%
GCTCTTGGTAAATTTGATGA+TGG - chr3_2:91101298-91101317 Msa0437110:intron 35.0%
!! TTGATGATGGACAATTTCCT+AGG - chr3_2:91101311-91101330 Msa0437110:CDS 35.0%
ATTTCCTAGGACAATTCAGT+TGG - chr3_2:91101324-91101343 Msa0437110:CDS 35.0%
TTTCCTAGGACAATTCAGTT+GGG - chr3_2:91101325-91101344 Msa0437110:CDS 35.0%
!! TATTGGTTGTGTTGATTAGG+TGG - chr3_2:91101571-91101590 Msa0437110:intron 35.0%
ACACCTCAGAAAAGACTATA+TGG + chr3_2:91101628-91101647 None:intergenic 35.0%
! GGTTAAGTTTTCCTCAAGTT+CGG + chr3_2:91101786-91101805 None:intergenic 35.0%
GTGCATAATGTTTCTTTGAC+AGG - chr3_2:91101910-91101929 Msa0437110:intron 35.0%
TTGATGCTTATACACAGACA+AGG - chr3_2:91101945-91101964 Msa0437110:intron 35.0%
TGATGCTTATACACAGACAA+GGG - chr3_2:91101946-91101965 Msa0437110:intron 35.0%
! ATGATGCTTTTCCATGTTGA+TGG - chr3_2:91102367-91102386 Msa0437110:CDS 35.0%
TATTTCACCATCCATCAACA+TGG + chr3_2:91102381-91102400 None:intergenic 35.0%
GAAAGTCTACCCAGAAAACA+AGG - chr3_2:91100805-91100824 Msa0437110:three_prime_UTR 40.0%
GATCCTGCAAAGAAAGAGTT+TGG - chr3_2:91101082-91101101 Msa0437110:intron 40.0%
TCACCAAACTCTTTCTTTGC+AGG + chr3_2:91101088-91101107 None:intergenic 40.0%
GGAGATCCTATAAAACAAGC+TGG - chr3_2:91101163-91101182 Msa0437110:CDS 40.0%
TTCTCCAAGTAATCAAAGGC+AGG + chr3_2:91101277-91101296 None:intergenic 40.0%
GATTACTTGGAGAATGCTCT+TGG - chr3_2:91101283-91101302 Msa0437110:intron 40.0%
TTACCCAACTGAATTGTCCT+AGG + chr3_2:91101331-91101350 None:intergenic 40.0%
TAAAGCATGTGATGGTCTGA+TGG - chr3_2:91101445-91101464 Msa0437110:CDS 40.0%
TCAATCCATGCTGCAAGTTT+CGG + chr3_2:91101677-91101696 None:intergenic 40.0%
AACTTAACCTGTTCCGCAAA+TGG - chr3_2:91101797-91101816 Msa0437110:CDS 40.0%
ATCTCGTGTTAACGAGACAT+CGG + chr3_2:91101884-91101903 None:intergenic 40.0%
! CTCACAATGTTCTAACTTGC+AGG - chr3_2:91102101-91102120 Msa0437110:intron 40.0%
CAGGCTCAACAGTGAAAATT+GGG - chr3_2:91102120-91102139 Msa0437110:intron 40.0%
! CAGTGAAAATTGGGTTGCAA+TGG - chr3_2:91102129-91102148 Msa0437110:intron 40.0%
!! TGCTTTTCCATGTTGATGGA+TGG - chr3_2:91102371-91102390 Msa0437110:CDS 40.0%
GAAAGTCTACCCAGAAAACA+AGG - chr3_2:91100805-91100824 Msa0437110:three_prime_UTR 40.0%
GATCCTGCAAAGAAAGAGTT+TGG - chr3_2:91101082-91101101 Msa0437110:intron 40.0%
TCACCAAACTCTTTCTTTGC+AGG + chr3_2:91101088-91101107 None:intergenic 40.0%
GGAGATCCTATAAAACAAGC+TGG - chr3_2:91101163-91101182 Msa0437110:CDS 40.0%
TTCTCCAAGTAATCAAAGGC+AGG + chr3_2:91101277-91101296 None:intergenic 40.0%
GATTACTTGGAGAATGCTCT+TGG - chr3_2:91101283-91101302 Msa0437110:intron 40.0%
TTACCCAACTGAATTGTCCT+AGG + chr3_2:91101331-91101350 None:intergenic 40.0%
TAAAGCATGTGATGGTCTGA+TGG - chr3_2:91101445-91101464 Msa0437110:CDS 40.0%
TCAATCCATGCTGCAAGTTT+CGG + chr3_2:91101677-91101696 None:intergenic 40.0%
AACTTAACCTGTTCCGCAAA+TGG - chr3_2:91101797-91101816 Msa0437110:CDS 40.0%
ATCTCGTGTTAACGAGACAT+CGG + chr3_2:91101884-91101903 None:intergenic 40.0%
! CTCACAATGTTCTAACTTGC+AGG - chr3_2:91102101-91102120 Msa0437110:intron 40.0%
CAGGCTCAACAGTGAAAATT+GGG - chr3_2:91102120-91102139 Msa0437110:intron 40.0%
! CAGTGAAAATTGGGTTGCAA+TGG - chr3_2:91102129-91102148 Msa0437110:intron 40.0%
!! TGCTTTTCCATGTTGATGGA+TGG - chr3_2:91102371-91102390 Msa0437110:CDS 40.0%
GGCCTGTCTTGAAGGTTAAT+AGG + chr3_2:91100770-91100789 None:intergenic 45.0%
AGACAGGCCTGTTTGGTATA+AGG - chr3_2:91100781-91100800 Msa0437110:three_prime_UTR 45.0%
! GTATGTATAGGTGCCATCGT+TGG - chr3_2:91100896-91100915 Msa0437110:three_prime_UTR 45.0%
!!! TCGATTTTTTTTGGGGGTGC+AGG + chr3_2:91101023-91101042 None:intergenic 45.0%
AGGTCCTGCCTTTGATTACT+TGG - chr3_2:91101270-91101289 Msa0437110:intron 45.0%
! GAAACTTGCAGCATGGATTG+AGG - chr3_2:91101676-91101695 Msa0437110:intron 45.0%
AGGTCATCAATCCGAACTTG+AGG - chr3_2:91101772-91101791 Msa0437110:CDS 45.0%
ACCTGTTCCGCAAATGGAAA+CGG - chr3_2:91101803-91101822 Msa0437110:CDS 45.0%
TCCGCAAATGGAAACGGAAA+TGG - chr3_2:91101809-91101828 Msa0437110:CDS 45.0%
CCCATTTCCGTTTCCATTTG+CGG + chr3_2:91101813-91101832 None:intergenic 45.0%
CCGCAAATGGAAACGGAAAT+GGG - chr3_2:91101810-91101829 Msa0437110:CDS 45.0%
! AGCTTGAGGACCATTTTCTG+TGG + chr3_2:91101836-91101855 None:intergenic 45.0%
AGAACTCTTGTCGAAGCTTG+AGG + chr3_2:91101850-91101869 None:intergenic 45.0%
TAACGAGACATCGGAGGTAA+TGG + chr3_2:91101875-91101894 None:intergenic 45.0%
GCAGGCTCAACAGTGAAAAT+TGG - chr3_2:91102119-91102138 Msa0437110:intron 45.0%
GGCCTGTCTTGAAGGTTAAT+AGG + chr3_2:91100770-91100789 None:intergenic 45.0%
AGACAGGCCTGTTTGGTATA+AGG - chr3_2:91100781-91100800 Msa0437110:three_prime_UTR 45.0%
! GTATGTATAGGTGCCATCGT+TGG - chr3_2:91100896-91100915 Msa0437110:three_prime_UTR 45.0%
!!! TCGATTTTTTTTGGGGGTGC+AGG + chr3_2:91101023-91101042 None:intergenic 45.0%
AGGTCCTGCCTTTGATTACT+TGG - chr3_2:91101270-91101289 Msa0437110:intron 45.0%
! GAAACTTGCAGCATGGATTG+AGG - chr3_2:91101676-91101695 Msa0437110:intron 45.0%
AGGTCATCAATCCGAACTTG+AGG - chr3_2:91101772-91101791 Msa0437110:CDS 45.0%
ACCTGTTCCGCAAATGGAAA+CGG - chr3_2:91101803-91101822 Msa0437110:CDS 45.0%
TCCGCAAATGGAAACGGAAA+TGG - chr3_2:91101809-91101828 Msa0437110:CDS 45.0%
CCCATTTCCGTTTCCATTTG+CGG + chr3_2:91101813-91101832 None:intergenic 45.0%
CCGCAAATGGAAACGGAAAT+GGG - chr3_2:91101810-91101829 Msa0437110:CDS 45.0%
! AGCTTGAGGACCATTTTCTG+TGG + chr3_2:91101836-91101855 None:intergenic 45.0%
AGAACTCTTGTCGAAGCTTG+AGG + chr3_2:91101850-91101869 None:intergenic 45.0%
TAACGAGACATCGGAGGTAA+TGG + chr3_2:91101875-91101894 None:intergenic 45.0%
GCAGGCTCAACAGTGAAAAT+TGG - chr3_2:91102119-91102138 Msa0437110:intron 45.0%
ACCTTCAAGACAGGCCTGTT+TGG - chr3_2:91100774-91100793 Msa0437110:three_prime_UTR 50.0%
ACCAAACAGGCCTGTCTTGA+AGG + chr3_2:91100778-91100797 None:intergenic 50.0%
TGCCATCGTTGGAGCACAAT+GGG - chr3_2:91100907-91100926 Msa0437110:three_prime_UTR 50.0%
TTCCCATTGTGCTCCAACGA+TGG + chr3_2:91100912-91100931 None:intergenic 50.0%
ATCGTTGGAGCACAATGGGA+AGG - chr3_2:91100911-91100930 Msa0437110:three_prime_UTR 50.0%
GAGCACAATGGGAAGGTCTT+GGG - chr3_2:91100918-91100937 Msa0437110:three_prime_UTR 50.0%
CTCACACTGGGAACTAGAGA+TGG + chr3_2:91100990-91101009 None:intergenic 50.0%
!! GGAGAGTGCTTTCTCACACT+GGG + chr3_2:91101002-91101021 None:intergenic 50.0%
!! AGGAGAGTGCTTTCTCACAC+TGG + chr3_2:91101003-91101022 None:intergenic 50.0%
! TGTGATGGTCTGATGGATCG+TGG - chr3_2:91101452-91101471 Msa0437110:CDS 50.0%
TCCGTTTCCATTTGCGGAAC+AGG + chr3_2:91101807-91101826 None:intergenic 50.0%
CGGAAATGGGCCACAGAAAA+TGG - chr3_2:91101823-91101842 Msa0437110:CDS 50.0%
TCGTGTTAACGAGACATCGG+AGG + chr3_2:91101881-91101900 None:intergenic 50.0%
TTGCAATGGCACCCTTTGAG+AGG - chr3_2:91102143-91102162 Msa0437110:CDS 50.0%
GCAGCAGTCTACCTCTCAAA+GGG + chr3_2:91102157-91102176 None:intergenic 50.0%
ACCTTCAAGACAGGCCTGTT+TGG - chr3_2:91100774-91100793 Msa0437110:three_prime_UTR 50.0%
ACCAAACAGGCCTGTCTTGA+AGG + chr3_2:91100778-91100797 None:intergenic 50.0%
TGCCATCGTTGGAGCACAAT+GGG - chr3_2:91100907-91100926 Msa0437110:three_prime_UTR 50.0%
TTCCCATTGTGCTCCAACGA+TGG + chr3_2:91100912-91100931 None:intergenic 50.0%
ATCGTTGGAGCACAATGGGA+AGG - chr3_2:91100911-91100930 Msa0437110:three_prime_UTR 50.0%
GAGCACAATGGGAAGGTCTT+GGG - chr3_2:91100918-91100937 Msa0437110:three_prime_UTR 50.0%
CTCACACTGGGAACTAGAGA+TGG + chr3_2:91100990-91101009 None:intergenic 50.0%
!! GGAGAGTGCTTTCTCACACT+GGG + chr3_2:91101002-91101021 None:intergenic 50.0%
!! AGGAGAGTGCTTTCTCACAC+TGG + chr3_2:91101003-91101022 None:intergenic 50.0%
! TGTGATGGTCTGATGGATCG+TGG - chr3_2:91101452-91101471 Msa0437110:CDS 50.0%
TCCGTTTCCATTTGCGGAAC+AGG + chr3_2:91101807-91101826 None:intergenic 50.0%
CGGAAATGGGCCACAGAAAA+TGG - chr3_2:91101823-91101842 Msa0437110:CDS 50.0%
TCGTGTTAACGAGACATCGG+AGG + chr3_2:91101881-91101900 None:intergenic 50.0%
TTGCAATGGCACCCTTTGAG+AGG - chr3_2:91102143-91102162 Msa0437110:CDS 50.0%
GCAGCAGTCTACCTCTCAAA+GGG + chr3_2:91102157-91102176 None:intergenic 50.0%
GTGCCATCGTTGGAGCACAA+TGG - chr3_2:91100906-91100925 Msa0437110:three_prime_UTR 55.0%
GGAGCACAATGGGAAGGTCT+TGG - chr3_2:91100917-91100936 Msa0437110:three_prime_UTR 55.0%
GAAGGCCGAAACTTGCAGCA+TGG - chr3_2:91101669-91101688 Msa0437110:intron 55.0%
GGCAGCAGTCTACCTCTCAA+AGG + chr3_2:91102158-91102177 None:intergenic 55.0%
GTGCCATCGTTGGAGCACAA+TGG - chr3_2:91100906-91100925 Msa0437110:three_prime_UTR 55.0%
GGAGCACAATGGGAAGGTCT+TGG - chr3_2:91100917-91100936 Msa0437110:three_prime_UTR 55.0%
GAAGGCCGAAACTTGCAGCA+TGG - chr3_2:91101669-91101688 Msa0437110:intron 55.0%
GGCAGCAGTCTACCTCTCAA+AGG + chr3_2:91102158-91102177 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 91100734 91102413 91100734 ID=Msa0437110;Name=Msa0437110
chr3_2 mRNA 91100734 91102413 91100734 ID=Msa0437110-mRNA-1;Parent=Msa0437110;Name=Msa0437110-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.16|0.14|1|1|1|7|279|251
chr3_2 exon 91100734 91101024 91100734 ID=Msa0437110-mRNA-1:exon:27241;Parent=Msa0437110-mRNA-1
chr3_2 exon 91101135 91101215 91101135 ID=Msa0437110-mRNA-1:exon:27240;Parent=Msa0437110-mRNA-1
chr3_2 exon 91101303 91101557 91101303 ID=Msa0437110-mRNA-1:exon:27239;Parent=Msa0437110-mRNA-1
chr3_2 exon 91101763 91101875 91101763 ID=Msa0437110-mRNA-1:exon:27238;Parent=Msa0437110-mRNA-1
chr3_2 exon 91101963 91102065 91101963 ID=Msa0437110-mRNA-1:exon:27237;Parent=Msa0437110-mRNA-1
chr3_2 exon 91102143 91102241 91102143 ID=Msa0437110-mRNA-1:exon:27236;Parent=Msa0437110-mRNA-1
chr3_2 exon 91102321 91102413 91102321 ID=Msa0437110-mRNA-1:exon:27235;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91102321 91102413 91102321 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91102143 91102241 91102143 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91101963 91102065 91101963 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91101763 91101875 91101763 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91101303 91101557 91101303 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91101135 91101215 91101135 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 CDS 91101013 91101024 91101013 ID=Msa0437110-mRNA-1:cds;Parent=Msa0437110-mRNA-1
chr3_2 three_prime_UTR 91100734 91101012 91100734 ID=Msa0437110-mRNA-1:three_prime_utr;Parent=Msa0437110-mRNA-1
Gene Sequence

>Msa0437110

ATGCAGGGACTACAAGACAAAATCAAATTGGTTCCTATTAACCTTCAAGACAGGCCTGTTTGGTATAAGGAGAAAGTCTACCCAGAAAACAAGGTGCCATCGTTGGAGCACAATGGGAAGGTCTTGGGAGAAAGTCTCGATTTGATCAGTTACATAGATGTCAACTTTGAGGGGCCATCTCTAGTTCCCAGTGATCCTGCAAAGAAAGAGTTTGGTGATCAGTTGATTTCTCATGTTGATACATTCACCAAAGATCTGTACTCTTCATTGAAAGGAGATCCTATAAAACAAGCTGGTCCTGCCTTTGATTACTTGGAGAATGCTCTTGGTAAATTTGATGATGGACAATTTCCTAGGACAATTCAGTTGGGTAAGTTGAAATTAAGAGACTTGCCATTTTTCTTTTCTGTGGATTTTGCCTATATTCCATTTGTTGAAAGATTCCATATAGTCTTTTCTGAGGTGTTCAAGCATGATATAATAGAAGGAAGGCCGAAACTTGCAGCATGGATTGAGGTACAGTTGAAACAGTTCTCTCTAAAATTGCAGTTTCCCACAATTGCATTCGTTTATGAATTGATTACAAATCAAGGTCATCAATCCGAACTTGAGGAAAACTTAACCTGTTCCGCAAATGGAAACGGAAATGGGCCACAGAAAATGGAGCTTAACAAGATTGATGCTTATACACAGACAAGGGCCGATCCAAATGAAATTGTTGATATTTTCAAGAAACGCTTTCTGGCTCAACAGTGA
Protein sequence

>Msa0437110

MQGLQDKIKLVPINLQDRPVWYKEKVYPENKVPSLEHNGKVLGESLDLISYIDVNFEGPSLVPSDPAKKEFGDQLISHVDTFTKDLYSSLKGDPIKQAGPAFDYLENALGKFDDGQFPRTIQLGKLKLRDLPFFFSVDFAYIPFVERFHIVFSEVFKHDIIEGRPKLAAWIEVQLKQFSLKLQFPTIAFVYELITNQGHQSELEENLTCSANGNGNGPQKMELNKIDAYTQTRADPNEIVDIFKKRFLAQQ*