AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1221020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221020 MtrunA17_Chr1g0209681 77.372 137 7 2 54 167 1 136 3.67e-59 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221020 AT1G57600.1 48.175 137 47 2 54 167 1 136 1.22e-33 126
Msa1221020 AT1G57600.2 48.175 137 47 2 54 167 1 136 1.22e-33 126
Msa1221020 AT1G57600.3 48.175 137 47 2 54 167 1 136 1.22e-33 126

Find 56 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGAATCTTTACACTTC+TGG 0.211962 8_1:-84467105 Msa1221020:CDS
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 8_1:+84467532 None:intergenic
CCTGTTCTTACACTTGTCTT+TGG 0.273288 8_1:-84467122 Msa1221020:CDS
GCTTAGTCTGAGATTAGTTT+TGG 0.281512 8_1:-84464736 Msa1221020:three_prime_UTR
GAGATCAGACTTCTTAGTTT+GGG 0.303634 8_1:+84467727 None:intergenic
AGAGATCAGACTTCTTAGTT+TGG 0.310750 8_1:+84467726 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 8_1:+84464999 None:intergenic
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 8_1:-84464788 Msa1221020:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 8_1:-84464461 Msa1221020:three_prime_UTR
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 8_1:+84467082 None:intergenic
AATCTCAGACTAAGCTGAAT+TGG 0.361937 8_1:+84464744 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 8_1:+84468223 None:intergenic
TACAAAATCATAGAATTATA+AGG 0.389269 8_1:+84464525 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 8_1:-84464442 Msa1221020:three_prime_UTR
ACAATTTAATGAGAAGTGAT+AGG 0.441583 8_1:-84464868 Msa1221020:three_prime_UTR
TTGGGAGATCGAAGGAAGAC+AGG 0.456175 8_1:+84467745 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 8_1:-84467011 Msa1221020:CDS
AAGTATCTGAACTTTGAAGT+AGG 0.473094 8_1:-84466068 Msa1221020:CDS
ATGCTCCATGCAAATAAGAT+AGG 0.474131 8_1:+84467006 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 8_1:-84467049 Msa1221020:CDS
AAATGTAAGTAAAGGAGATA+GGG 0.493237 8_1:-84464787 Msa1221020:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 8_1:-84464713 Msa1221020:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 8_1:+84467122 None:intergenic
AGTATCTGAACTTTGAAGTA+GGG 0.499224 8_1:-84466067 Msa1221020:intron
AGGAGCTAGGCTAAACCATC+AGG 0.501207 8_1:-84464848 Msa1221020:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 8_1:-84467686 Msa1221020:CDS
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 8_1:+84467533 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 8_1:+84464901 None:intergenic
TTCTCATTAAATTGTCGAGA+TGG 0.524264 8_1:+84464876 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 8_1:-84467091 Msa1221020:CDS
TTCTTCAATTTCAATGTGAG+AGG 0.534160 8_1:-84467065 Msa1221020:CDS
AAGAAATTCCACCTCCATCT+CGG 0.534799 8_1:-84464915 Msa1221020:three_prime_UTR
TTAAATTGCCGAGATGGAGG+TGG 0.549539 8_1:+84464907 None:intergenic
CTCTTAGCTTGGCAGTGCAT+AGG 0.552112 8_1:-84464567 Msa1221020:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 8_1:+84464833 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 8_1:-84467090 Msa1221020:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 8_1:-84464861 Msa1221020:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 8_1:-84467004 Msa1221020:intron
TTCTTAGTTTGGGAGATCGA+AGG 0.582704 8_1:+84467737 None:intergenic
TCATTAAATTGCCGAGATGG+AGG 0.583358 8_1:+84464904 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 8_1:-84467693 Msa1221020:CDS
TACTCCCATAGATTGTCTCA+AGG 0.588702 8_1:-84465004 Msa1221020:CDS
ACTCCCATAGATTGTCTCAA+GGG 0.598235 8_1:-84465003 Msa1221020:CDS
CATCATCAGGCGCTCCCTCG+CGG 0.610543 8_1:-84467474 Msa1221020:intron
TCAAACCTGGAAAACGGACG+CGG 0.612446 8_1:-84467680 Msa1221020:intron
CTCCCATAGATTGTCTCAAG+GGG 0.617721 8_1:-84465002 Msa1221020:CDS
GGCGAAAAGAACGAAGACAT+AGG 0.621764 8_1:-84465047 Msa1221020:CDS
ATCCCCTTGAGACAATCTAT+GGG 0.622438 8_1:+84465000 None:intergenic
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 8_1:-84467163 Msa1221020:CDS
TGAATCGAACCAAACAGCAA+AGG 0.643380 8_1:-84468232 Msa1221020:intron
ACAGTACATTGCGATGAACA+AGG 0.655075 8_1:-84467576 Msa1221020:intron
AGAAAACAAAAGCCAGACAA+CGG 0.663004 8_1:+84467037 None:intergenic
AAAGAAGAAACATACCGCGA+GGG 0.665018 8_1:+84467460 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 8_1:+84467010 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 8_1:-84467550 Msa1221020:CDS
AGGATGTCTCAGATGCTCAG+TGG 0.679281 8_1:-84467166 Msa1221020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAAATA+TGG + chr8_1:84466399-84466418 None:intergenic 10.0%
!! ATAAAATTATAAGTCTAAAT+AGG + chr8_1:84467418-84467437 None:intergenic 10.0%
!!! ATTTGTTTTTTATTTATCGT+CGG - chr8_1:84465254-84465273 Msa1221020:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG - chr8_1:84465763-84465782 Msa1221020:intron 15.0%
!! AAACAATGAAAATTAATCTT+TGG + chr8_1:84466127-84466146 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG - chr8_1:84466126-84466145 Msa1221020:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG + chr8_1:84466500-84466519 None:intergenic 15.0%
!! GATAATAGATGAATAAATAA+AGG - chr8_1:84467185-84467204 Msa1221020:intron 15.0%
!! CAAGTAGATATTTATTTAAA+AGG - chr8_1:84467374-84467393 Msa1221020:intron 15.0%
!!! ATTTTATTGATGAGAATATA+TGG - chr8_1:84467431-84467450 Msa1221020:intron 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG - chr8_1:84467562-84467581 Msa1221020:CDS 15.0%
!! TACAAAATCATAGAATTATA+AGG + chr8_1:84468108-84468127 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG - chr8_1:84468160-84468179 Msa1221020:intron 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr8_1:84464668-84464687 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG - chr8_1:84465821-84465840 Msa1221020:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG - chr8_1:84465920-84465939 Msa1221020:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG + chr8_1:84466208-84466227 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG - chr8_1:84466207-84466226 Msa1221020:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG - chr8_1:84466533-84466552 Msa1221020:intron 20.0%
!!! ATACAATTATTCATTTGTTG+AGG + chr8_1:84466783-84466802 None:intergenic 20.0%
!! ATATGAATAGTATATGATGT+TGG - chr8_1:84466816-84466835 Msa1221020:intron 20.0%
!! TATGAATAGTATATGATGTT+GGG - chr8_1:84466817-84466836 Msa1221020:intron 20.0%
!!! GTTCTTTAATTTTTACAAGT+TGG - chr8_1:84466839-84466858 Msa1221020:intron 20.0%
!! AAAAACAATTATTATGACAC+CGG + chr8_1:84466959-84466978 None:intergenic 20.0%
!!! TTTTTTTTGTTGTGTAATGT+AGG - chr8_1:84467271-84467290 Msa1221020:intron 20.0%
!! AAATAACACACAAATTCATT+AGG + chr8_1:84467558-84467577 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG - chr8_1:84467835-84467854 Msa1221020:intron 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG - chr8_1:84467991-84468010 Msa1221020:intron 20.0%
!!! TGATTTTGTTTCATAGATCT+AGG - chr8_1:84464575-84464594 Msa1221020:three_prime_UTR 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr8_1:84464683-84464702 Msa1221020:three_prime_UTR 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr8_1:84464849-84464868 Msa1221020:three_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr8_1:84465143-84465162 Msa1221020:intron 25.0%
! CTGAAATACTTACAAAGAAA+TGG + chr8_1:84465374-84465393 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG + chr8_1:84465444-84465463 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr8_1:84465444-84465463 Msa1221020:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG + chr8_1:84466023-84466042 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG + chr8_1:84466115-84466134 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG - chr8_1:84466239-84466258 Msa1221020:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG + chr8_1:84466393-84466412 None:intergenic 25.0%
! AGCATTATATATGCTCTAAT+TGG - chr8_1:84466748-84466767 Msa1221020:intron 25.0%
! TATTTGTGTTATATAGGTGT+CGG + chr8_1:84467012-84467031 None:intergenic 25.0%
! ATATGAAACTCTTGTATCTA+TGG + chr8_1:84467096-84467115 None:intergenic 25.0%
! TTGAGAAATGTTGATCAATT+AGG - chr8_1:84467119-84467138 Msa1221020:CDS 25.0%
! TGAGAAATGTTGATCAATTA+GGG - chr8_1:84467120-84467139 Msa1221020:CDS 25.0%
!!! TTTTTTACTATTTCAGCAAC+AGG + chr8_1:84467311-84467330 None:intergenic 25.0%
! ACAATTTAATGAGAAGTGAT+AGG - chr8_1:84467762-84467781 Msa1221020:CDS 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG - chr8_1:84467842-84467861 Msa1221020:intron 25.0%
! AAATGTAAGTAAAGGAGATA+GGG - chr8_1:84467843-84467862 Msa1221020:intron 25.0%
! AAACAAAAAAACTCAGAACA+AGG + chr8_1:84467981-84468000 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG - chr8_1:84468012-84468031 Msa1221020:intron 25.0%
! TATTGTAACTTTGGATTTGT+TGG - chr8_1:84468169-84468188 Msa1221020:intron 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr8_1:84464520-84464539 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr8_1:84464632-84464651 Msa1221020:three_prime_UTR 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr8_1:84464748-84464767 Msa1221020:three_prime_UTR 30.0%
CTACATAAACAGGTTATACA+TGG + chr8_1:84465203-84465222 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr8_1:84465213-84465232 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr8_1:84465282-84465301 Msa1221020:intron 30.0%
AATCATACATTGAGGTGATT+AGG + chr8_1:84465330-84465349 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr8_1:84465565-84465584 Msa1221020:intron 30.0%
TTATCTCTAACAGCAAGATA+AGG + chr8_1:84466045-84466064 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG + chr8_1:84466078-84466097 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG - chr8_1:84466158-84466177 Msa1221020:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG - chr8_1:84466312-84466331 Msa1221020:intron 30.0%
! ACTATTTATCTTTCAAGTCC+TGG - chr8_1:84466365-84466384 Msa1221020:intron 30.0%
! CATGAATGTTTGGAATTACA+AGG + chr8_1:84466426-84466445 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG + chr8_1:84466436-84466455 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG - chr8_1:84466562-84466581 Msa1221020:intron 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG - chr8_1:84466563-84466582 Msa1221020:intron 30.0%
ATGCTAAATACACTTGAACA+CGG - chr8_1:84466613-84466632 Msa1221020:intron 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG - chr8_1:84466637-84466656 Msa1221020:intron 30.0%
CGGTTGTATTTGTGTTATAT+AGG + chr8_1:84467018-84467037 None:intergenic 30.0%
GAGAAATGTTGATCAATTAG+GGG - chr8_1:84467121-84467140 Msa1221020:CDS 30.0%
TATTGATGAGAATATATGGC+AGG - chr8_1:84467435-84467454 Msa1221020:intron 30.0%
ATAAAACAACCTAACCAGAA+AGG + chr8_1:84467488-84467507 None:intergenic 30.0%
TTCTCATTAAATTGTCGAGA+TGG + chr8_1:84467757-84467776 None:intergenic 30.0%
! TTTTAGTCTAACTCTTAGCT+TGG - chr8_1:84468052-84468071 Msa1221020:intron 30.0%
TTGGATATTGTAACAATGAC+AGG - chr8_1:84468188-84468207 Msa1221020:intron 30.0%
AAACAACAGAAGGGCAAAAA+AGG - chr8_1:84464836-84464855 Msa1221020:three_prime_UTR 35.0%
GAGATCAGACTTCTTAGTTT+GGG + chr8_1:84464906-84464925 None:intergenic 35.0%
AGAGATCAGACTTCTTAGTT+TGG + chr8_1:84464907-84464926 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr8_1:84465029-84465048 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr8_1:84465338-84465357 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr8_1:84465525-84465544 Msa1221020:intron 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr8_1:84465596-84465615 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr8_1:84465623-84465642 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr8_1:84465627-84465646 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr8_1:84465626-84465645 Msa1221020:intron 35.0%
GTCGAGTGACAAATGAAAAT+AGG + chr8_1:84466347-84466366 None:intergenic 35.0%
! ACACGGATTTTGCATTTTAG+TGG - chr8_1:84466630-84466649 Msa1221020:intron 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG - chr8_1:84466638-84466657 Msa1221020:intron 35.0%
TAACACAAATACAACCGTGT+CGG - chr8_1:84467021-84467040 Msa1221020:CDS 35.0%
TTCTCATTAAATTGCCGAGA+TGG + chr8_1:84467732-84467751 None:intergenic 35.0%
AATCTCAGACTAAGCTGAAT+TGG + chr8_1:84467889-84467908 None:intergenic 35.0%
!!! GCTTAGTCTGAGATTAGTTT+TGG - chr8_1:84467894-84467913 Msa1221020:intron 35.0%
AATAGACTAAGTTGTGAGAC+TGG - chr8_1:84467917-84467936 Msa1221020:intron 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG - chr8_1:84467924-84467943 Msa1221020:intron 35.0%
! GCATAGGCTATGTTTTACTT+TGG - chr8_1:84468079-84468098 Msa1221020:intron 35.0%
TGAATCGAACCAAACAGCAA+AGG - chr8_1:84464398-84464417 Msa1221020:three_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG + chr8_1:84464410-84464429 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG + chr8_1:84464427-84464446 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr8_1:84464481-84464500 Msa1221020:three_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr8_1:84464530-84464549 Msa1221020:three_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG + chr8_1:84464737-84464756 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr8_1:84464827-84464846 Msa1221020:three_prime_UTR 40.0%
! TTCTTAGTTTGGGAGATCGA+AGG + chr8_1:84464896-84464915 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr8_1:84464937-84464956 Msa1221020:three_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr8_1:84464944-84464963 Msa1221020:three_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr8_1:84465054-84465073 Msa1221020:intron 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr8_1:84465100-84465119 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr8_1:84465101-84465120 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG + chr8_1:84465173-84465192 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr8_1:84465511-84465530 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr8_1:84465508-84465527 Msa1221020:intron 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr8_1:84465540-84465559 Msa1221020:intron 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr8_1:84465551-84465570 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr8_1:84465619-84465638 Msa1221020:intron 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr8_1:84465631-84465650 Msa1221020:intron 40.0%
TTAAATATGGCACAGGAACC+AGG + chr8_1:84466386-84466405 None:intergenic 40.0%
CGTTCCTTATCTATGAAGCA+CGG - chr8_1:84466867-84466886 Msa1221020:intron 40.0%
GTATCCGTGCTTCATAGATA+AGG + chr8_1:84466874-84466893 None:intergenic 40.0%
TACTTCGACACTCTTACGAT+CGG - chr8_1:84466983-84467002 Msa1221020:intron 40.0%
ATAGGAGACTTGTGTTTCAG+CGG + chr8_1:84467400-84467419 None:intergenic 40.0%
AACAACCTAACCAGAAAGGA+AGG + chr8_1:84467484-84467503 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG - chr8_1:84467626-84467645 Msa1221020:intron 40.0%
ACTCCCATAGATTGTCTCAA+GGG - chr8_1:84467627-84467646 Msa1221020:intron 40.0%
ATCCCCTTGAGACAATCTAT+GGG + chr8_1:84467633-84467652 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG + chr8_1:84467634-84467653 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG - chr8_1:84467715-84467734 Msa1221020:CDS 40.0%
TCATTAAATTGCCGAGATGG+AGG + chr8_1:84467729-84467748 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG - chr8_1:84467769-84467788 Msa1221020:CDS 40.0%
TCTGATGTATAAGCTCCTGA+TGG + chr8_1:84467800-84467819 None:intergenic 40.0%
AAAGGTAACCGAATTCGCGA+AGG - chr8_1:84464416-84464435 Msa1221020:three_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr8_1:84464544-84464563 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr8_1:84464813-84464832 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr8_1:84464825-84464844 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr8_1:84464826-84464845 Msa1221020:three_prime_UTR 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr8_1:84464982-84465001 Msa1221020:exon 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr8_1:84465080-84465099 Msa1221020:intron 45.0%
GAAAGAAGAAACATACCGCG+AGG + chr8_1:84465174-84465193 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr8_1:84465293-84465312 Msa1221020:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr8_1:84465315-84465334 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr8_1:84465539-84465558 Msa1221020:intron 45.0%
GCATCTGAGGGGAAACTAAA+TGG - chr8_1:84466292-84466311 Msa1221020:intron 45.0%
CTGAGGGGAAACTAAATGGT+TGG - chr8_1:84466296-84466315 Msa1221020:intron 45.0%
TATGAAGCACGGATACCTCT+AGG - chr8_1:84466878-84466897 Msa1221020:CDS 45.0%
! CCGGTTCGACACATATCTTA+CGG + chr8_1:84466940-84466959 None:intergenic 45.0%
CCGTAAGATATGTGTCGAAC+CGG - chr8_1:84466937-84466956 Msa1221020:intron 45.0%
TTTGTCTGAGTTGTCCGACA+CGG + chr8_1:84467038-84467057 None:intergenic 45.0%
ACTCTTGTATCTATGGTGCG+CGG + chr8_1:84467089-84467108 None:intergenic 45.0%
AAGAAAACGCATGTGAGCGT+TGG - chr8_1:84467329-84467348 Msa1221020:intron 45.0%
GGACTTTGCTAAGACTTGAG+AGG - chr8_1:84467456-84467475 Msa1221020:intron 45.0%
TTGAGAGGATCCTTCCTTTC+TGG - chr8_1:84467471-84467490 Msa1221020:intron 45.0%
!! AGGATCCTTCCTTTCTGGTT+AGG - chr8_1:84467476-84467495 Msa1221020:CDS 45.0%
GGCGAAAAGAACGAAGACAT+AGG - chr8_1:84467583-84467602 Msa1221020:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG - chr8_1:84467628-84467647 Msa1221020:intron 45.0%
TTAAATTGCCGAGATGGAGG+TGG + chr8_1:84467726-84467745 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG - chr8_1:84467930-84467949 Msa1221020:intron 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG - chr8_1:84467933-84467952 Msa1221020:intron 45.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr8_1:84464541-84464560 Msa1221020:three_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr8_1:84464688-84464707 Msa1221020:three_prime_UTR 50.0%
GCCGGAATAGCAAATCTCGT+TGG - chr8_1:84464733-84464752 Msa1221020:three_prime_UTR 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr8_1:84464888-84464907 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr8_1:84464950-84464969 Msa1221020:three_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr8_1:84464980-84464999 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr8_1:84464981-84465000 Msa1221020:exon 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr8_1:84464983-84465002 Msa1221020:exon 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr8_1:84465294-84465313 Msa1221020:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr8_1:84465314-84465333 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr8_1:84465464-84465483 Msa1221020:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr8_1:84465467-84465486 Msa1221020:intron 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr8_1:84465478-84465497 Msa1221020:intron 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr8_1:84465581-84465600 Msa1221020:intron 50.0%
ACAGTATCCGACAGCATCTG+AGG - chr8_1:84466279-84466298 Msa1221020:intron 50.0%
CAGTATCCGACAGCATCTGA+GGG - chr8_1:84466280-84466299 Msa1221020:intron 50.0%
AGTATCCGACAGCATCTGAG+GGG - chr8_1:84466281-84466300 Msa1221020:intron 50.0%
AGTTTCCCCTCAGATGCTGT+CGG + chr8_1:84466289-84466308 None:intergenic 50.0%
!! TTACGGTGTCATACGCGTGT+CGG + chr8_1:84466923-84466942 None:intergenic 50.0%
AGGAGCTAGGCTAAACCATC+AGG - chr8_1:84467782-84467801 Msa1221020:CDS 50.0%
! CTCTTAGCTTGGCAGTGCAT+AGG - chr8_1:84468063-84468082 Msa1221020:intron 50.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr8_1:84464506-84464525 Msa1221020:three_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG + chr8_1:84464729-84464748 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG + chr8_1:84464732-84464751 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr8_1:84464997-84465016 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr8_1:84465298-84465317 Msa1221020:intron 55.0%
!! GCACGGATACCTCTAGGACT+AGG - chr8_1:84466884-84466903 Msa1221020:CDS 55.0%
!! CTCTAGGACTAGGCATGTCG+CGG - chr8_1:84466894-84466913 Msa1221020:CDS 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr8_1:84464691-84464710 Msa1221020:three_prime_UTR 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr8_1:84464958-84464977 None:intergenic 60.0%
CGCGACATGCCTAGTCCTAG+AGG + chr8_1:84466896-84466915 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG + chr8_1:84464725-84464744 None:intergenic 65.0%
CATCATCAGGCGCTCCCTCG+CGG - chr8_1:84465156-84465175 Msa1221020:intron 65.0%
TATTCCGGCGAGGTGGCCGG+TGG + chr8_1:84464722-84464741 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG + chr8_1:84464705-84464724 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG - chr8_1:84464703-84464722 Msa1221020:three_prime_UTR 80.0%
! GCCACCACCGGCCACCTCGC+CGG - chr8_1:84464715-84464734 Msa1221020:three_prime_UTR 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG + chr8_1:84464719-84464738 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84464397 84468255 84464397 ID=Msa1221020;Name=Msa1221020
chr8_1 mRNA 84464397 84468255 84464397 ID=Msa1221020-mRNA-1;Parent=Msa1221020;Name=Msa1221020-mRNA-1;_AED=0.34;_eAED=0.37;_QI=0|0.33|0.42|1|0.5|0.57|7|590|209
chr8_1 exon 84464397 84465068 84464397 ID=Msa1221020-mRNA-1:exon:30331;Parent=Msa1221020-mRNA-1
chr8_1 exon 84466068 84466114 84466068 ID=Msa1221020-mRNA-1:exon:30330;Parent=Msa1221020-mRNA-1
chr8_1 exon 84466868 84466916 84466868 ID=Msa1221020-mRNA-1:exon:30329;Parent=Msa1221020-mRNA-1
chr8_1 exon 84467005 84467186 84467005 ID=Msa1221020-mRNA-1:exon:30328;Parent=Msa1221020-mRNA-1
chr8_1 exon 84467475 84467594 84467475 ID=Msa1221020-mRNA-1:exon:30327;Parent=Msa1221020-mRNA-1
chr8_1 exon 84467681 84467807 84467681 ID=Msa1221020-mRNA-1:exon:30326;Parent=Msa1221020-mRNA-1
chr8_1 exon 84468233 84468255 84468233 ID=Msa1221020-mRNA-1:exon:30325;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84468233 84468255 84468233 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84467681 84467807 84467681 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84467475 84467594 84467475 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84467005 84467186 84467005 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84466868 84466916 84466868 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84466068 84466114 84466068 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 CDS 84464987 84465068 84464987 ID=Msa1221020-mRNA-1:cds;Parent=Msa1221020-mRNA-1
chr8_1 three_prime_UTR 84464397 84464986 84464397 ID=Msa1221020-mRNA-1:three_prime_utr;Parent=Msa1221020-mRNA-1
Gene Sequence

>Msa1221020

ATGAATCGAACCAAACAGCAAAGAAGGGCAAAAAAGGAAGAAAAAAATGGAGTAAAGCTTTCTCCTGTCTTCCTTCGATCTCCCAAACTAAGAAGTCTGATCTCTCTCGTCGTCGTCAACAACTGTACTCAAACCTGGAAAACGGACGCGTACATTGCGATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGAAACTTATTTTGTCATGGAATATTAAGTATCTGAACTTTGAAGTAGGGCGAAAAGAACGAAGACATAGGAGACTCGTGCTTATAATAATTACTCCCATAGATTGTCTCAAGGGGATATTCTTTACTTGA
Protein sequence

>Msa1221020

MNRTKQQRRAKKEEKNGVKLSPVFLRSPKLRSLISLVVVNNCTQTWKTDAYIAMNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKKLILSWNIKYLNFEVGRKERRHRRLVLIIITPIDCLKGIFFT*