AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0876440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0876440 MtrunA17_Chr1g0209681 96.296 81 3 0 15 95 56 136 5.70e-46 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0876440 AT1G57600.1 62.195 82 31 0 19 100 60 141 2.66e-30 112
Msa0876440 AT1G57600.2 62.195 82 31 0 19 100 60 141 2.66e-30 112
Msa0876440 AT1G57600.3 62.195 82 31 0 19 100 60 141 2.66e-30 112

Find 105 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAGGATCCTTCCTTTC+TGG 0.164123 6_1:-14416097 Msa0876440:three_prime_UTR
CAAGTAGATATTTATTTAAA+AGG 0.207136 6_1:-14416194 Msa0876440:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 6_1:-14418043 Msa0876440:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 6_1:-14417256 Msa0876440:intron
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 6_1:+14418480 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 6_1:-14418597 Msa0876440:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 6_1:-14418060 Msa0876440:CDS
GCTTAGTGTGAGATTAGTTT+TGG 0.293666 6_1:-14415686 Msa0876440:three_prime_UTR
TTCTTCCTCCTTCGCGAATT+CGG 0.299784 6_1:+14419153 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 6_1:+14415949 None:intergenic
ATAAAATTATAAGTCTAAAT+AGG 0.331713 6_1:+14416153 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 6_1:-14419221 Msa0876440:five_prime_UTR
AGGATCCTTCCTTTCTGGTT+AGG 0.351041 6_1:-14416092 Msa0876440:intron
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 6_1:-14415738 Msa0876440:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 6_1:-14415411 Msa0876440:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 6_1:-14417276 Msa0876440:exon
AATCTCACACTAAGCTGAAT+TGG 0.358285 6_1:+14415694 None:intergenic
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 6_1:+14418020 None:intergenic
GAAAAGGGAAAAGGGAAAAG+TGG 0.365827 6_1:+14419406 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 6_1:+14419170 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 6_1:+14419227 None:intergenic
GAGAGATCAGACTTCTTAGT+TGG 0.380941 6_1:+14418673 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 6_1:-14418596 Msa0876440:five_prime_UTR
TACAAAATCATAGAATTATA+AGG 0.389269 6_1:+14415475 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 6_1:+14419228 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 6_1:+14419398 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 6_1:+14418552 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 6_1:+14419397 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 6_1:+14419361 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 6_1:-14415392 Msa0876440:three_prime_UTR
TCAATTTAATGAGAAGTGAT+AGG 0.441583 6_1:-14415818 Msa0876440:three_prime_UTR
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 6_1:+14419390 None:intergenic
AAAAGGGAAAAGGGAAAAGT+GGG 0.469224 6_1:+14419407 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 6_1:-14417949 Msa0876440:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 6_1:+14419036 None:intergenic
ATGCTCCATGCAAATAAGAT+AGG 0.474131 6_1:+14417944 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 6_1:+14418692 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 6_1:-14417987 Msa0876440:CDS
AGAGATCAGACTTCTTAGTT+GGG 0.483449 6_1:+14418674 None:intergenic
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 6_1:+14419380 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 6_1:+14419315 None:intergenic
AAATGTAAGTAAAGGAGATA+GGG 0.493237 6_1:-14415737 Msa0876440:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 6_1:-14415663 Msa0876440:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 6_1:+14418060 None:intergenic
AGGAGCTAGGCTAAACCATC+AGG 0.501207 6_1:-14415798 Msa0876440:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 6_1:-14418634 Msa0876440:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 6_1:+14418481 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 6_1:+14415851 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 6_1:+14419356 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 6_1:+14419242 None:intergenic
ACATACCGCGAGAAAGTTGA+AGG 0.522314 6_1:+14418407 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 6_1:-14418029 Msa0876440:CDS
TATTGATGAGAATATATGGC+AGG 0.526440 6_1:-14416133 Msa0876440:three_prime_UTR
CTCTTGCGATTCCACGATTG+GGG 0.530968 6_1:-14418595 Msa0876440:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 6_1:-14418003 Msa0876440:CDS
AAGAAATTCCACCTCCATCT+CGG 0.534799 6_1:-14415865 Msa0876440:three_prime_UTR
AAGAAAACGCATGTGAGCGT+TGG 0.536923 6_1:-14416239 Msa0876440:three_prime_UTR
TTCTCATTAAATTGACGAGA+TGG 0.542657 6_1:+14415826 None:intergenic
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 6_1:+14419381 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 6_1:+14419391 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 6_1:+14415857 None:intergenic
CTCTTAGCTTGGCAGTGCAT+AGG 0.552112 6_1:-14415517 Msa0876440:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 6_1:+14415783 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 6_1:-14418028 Msa0876440:CDS
GGTAACCGAATTCGCGAAGG+AGG 0.561943 6_1:-14419158 Msa0876440:five_prime_UTR
ATAAATTGTTCGATTGAGAA+CGG 0.563409 6_1:+14419352 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 6_1:+14419410 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 6_1:-14417272 Msa0876440:exon
ACAGTATCCGACAGCATCTG+AGG 0.568896 6_1:-14417289 Msa0876440:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 6_1:-14415811 Msa0876440:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 6_1:-14417942 Msa0876440:intron
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 6_1:-14419071 Msa0876440:five_prime_UTR
AACAACCTAACCAGAAAGGA+AGG 0.580432 6_1:+14416087 None:intergenic
CGCTCCCTTCAACTTTCTCG+CGG 0.582697 6_1:-14418412 Msa0876440:intron
TCATTAAATTGCCGAGATGG+AGG 0.583358 6_1:+14415854 None:intergenic
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 6_1:+14418601 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 6_1:-14418641 Msa0876440:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 6_1:-14415954 Msa0876440:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 6_1:+14418584 None:intergenic
CAGTATCCGACAGCATCTGA+GGG 0.590060 6_1:-14417288 Msa0876440:CDS
AGTTTCCCCTCAGATGCTGT+CGG 0.590938 6_1:+14417282 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 6_1:+14419382 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 6_1:-14415953 Msa0876440:three_prime_UTR
ATAAAACAACCTAACCAGAA+AGG 0.600525 6_1:+14416083 None:intergenic
CTTCTTAGTTGGGAGATCGA+AGG 0.610786 6_1:+14418684 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 6_1:-14418628 Msa0876440:five_prime_UTR
GGACTTTGCTAAGACTTGAG+AGG 0.614168 6_1:-14416112 Msa0876440:three_prime_UTR
ATAGGAGACTTGTGTTTCAG+CGG 0.616380 6_1:+14416171 None:intergenic
CTCCCATAGATTGTCTCAAG+GGG 0.617721 6_1:-14415952 Msa0876440:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 6_1:-14415997 Msa0876440:three_prime_UTR
ATCCCCTTGAGACAATCTAT+GGG 0.622438 6_1:+14415950 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 6_1:-14419096 Msa0876440:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 6_1:-14418101 Msa0876440:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 6_1:-14419161 Msa0876440:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 6_1:-14419179 Msa0876440:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 6_1:+14419411 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 6_1:+14419306 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 6_1:+14419300 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 6_1:-14418524 Msa0876440:five_prime_UTR
AGAAAACAAAAGCCAGACAA+CGG 0.663004 6_1:+14417975 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 6_1:+14417948 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 6_1:-14418498 Msa0876440:five_prime_UTR
AGGATGTCTCAGATGCTCAG+TGG 0.679281 6_1:-14418104 Msa0876440:CDS
CATACCGCGAGAAAGTTGAA+GGG 0.694597 6_1:+14418408 None:intergenic
AGTATCCGACAGCATCTGAG+GGG 0.723113 6_1:-14417287 Msa0876440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAATTA+TGG + chr6_1:14417626-14417645 None:intergenic 10.0%
!! ATAAAATTATAAGTCTAAAT+AGG + chr6_1:14418645-14418664 None:intergenic 10.0%
!!! TGAAAAGTGAATTTTTTTTT+AGG + chr6_1:14415360-14415379 None:intergenic 15.0%
!!! ATTTGTTTTTTATTTATCGT+CGG - chr6_1:14416481-14416500 Msa0876440:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG - chr6_1:14416990-14417009 Msa0876440:intron 15.0%
!! AAACAATGAAAATTAATCTT+TGG + chr6_1:14417354-14417373 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG - chr6_1:14417353-14417372 Msa0876440:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG + chr6_1:14417727-14417746 None:intergenic 15.0%
!! GATAATAGATGAATAAATAA+AGG - chr6_1:14418412-14418431 Msa0876440:intron 15.0%
!! CAAGTAGATATTTATTTAAA+AGG - chr6_1:14418601-14418620 Msa0876440:five_prime_UTR 15.0%
!!! ATTTTATTGATGAGAATATA+TGG - chr6_1:14418658-14418677 Msa0876440:five_prime_UTR 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG - chr6_1:14418777-14418796 Msa0876440:intron 15.0%
!! TACAAAATCATAGAATTATA+AGG + chr6_1:14419323-14419342 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG - chr6_1:14419375-14419394 Msa0876440:five_prime_UTR 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr6_1:14415886-14415905 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG - chr6_1:14417048-14417067 Msa0876440:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG - chr6_1:14417147-14417166 Msa0876440:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG + chr6_1:14417435-14417454 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG - chr6_1:14417434-14417453 Msa0876440:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG - chr6_1:14417760-14417779 Msa0876440:intron 20.0%
!!! ATACAATTATTCATTTGTTG+AGG + chr6_1:14418010-14418029 None:intergenic 20.0%
!! ATATGAATAGTATATGATGT+TGG - chr6_1:14418043-14418062 Msa0876440:CDS 20.0%
!! TATGAATAGTATATGATGTT+GGG - chr6_1:14418044-14418063 Msa0876440:CDS 20.0%
!!! GTTCTTTAATTTTTACAAGT+TGG - chr6_1:14418066-14418085 Msa0876440:CDS 20.0%
!! AAAAACAATTATTATGACAC+CGG + chr6_1:14418186-14418205 None:intergenic 20.0%
!! TATTTATGTTATATAGGTGT+CGG + chr6_1:14418239-14418258 None:intergenic 20.0%
!!! TTTTTTTTGTTGTGTAATGT+AGG - chr6_1:14418498-14418517 Msa0876440:five_prime_UTR 20.0%
!! AAATAACACACAAATTCATT+AGG + chr6_1:14418773-14418792 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG - chr6_1:14419050-14419069 Msa0876440:five_prime_UTR 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG - chr6_1:14419206-14419225 Msa0876440:five_prime_UTR 20.0%
! ATAAATTGTTCGATTGAGAA+CGG + chr6_1:14415446-14415465 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG - chr6_1:14415793-14415812 Msa0876440:three_prime_UTR 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr6_1:14415901-14415920 Msa0876440:three_prime_UTR 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr6_1:14416067-14416086 Msa0876440:intron 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr6_1:14416360-14416379 Msa0876440:intron 25.0%
! TTACACATAACAAACAGAAT+AGG + chr6_1:14416671-14416690 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr6_1:14416671-14416690 Msa0876440:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG + chr6_1:14417250-14417269 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG + chr6_1:14417342-14417361 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG - chr6_1:14417466-14417485 Msa0876440:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG - chr6_1:14417592-14417611 Msa0876440:intron 25.0%
! ATAAAACTTAATTATGGCAC+AGG + chr6_1:14417620-14417639 None:intergenic 25.0%
! AGCATTATATATGCTCTAAT+TGG - chr6_1:14417975-14417994 Msa0876440:CDS 25.0%
! CGGTTGTATTTATGTTATAT+AGG + chr6_1:14418245-14418264 None:intergenic 25.0%
! TAACATAAATACAACCGTAT+CGG - chr6_1:14418248-14418267 Msa0876440:intron 25.0%
! ATATGAAACTCTTGTATCTA+TGG + chr6_1:14418323-14418342 None:intergenic 25.0%
! TTGAGAAATGTTGATCAATT+AGG - chr6_1:14418346-14418365 Msa0876440:intron 25.0%
! TGAGAAATGTTGATCAATTA+GGG - chr6_1:14418347-14418366 Msa0876440:intron 25.0%
!!! TTTTTTACTATTTCAGCAAC+AGG + chr6_1:14418538-14418557 None:intergenic 25.0%
! TCAATTTAATGAGAAGTGAT+AGG - chr6_1:14418977-14418996 Msa0876440:intron 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG - chr6_1:14419057-14419076 Msa0876440:five_prime_UTR 25.0%
! AAATGTAAGTAAAGGAGATA+GGG - chr6_1:14419058-14419077 Msa0876440:five_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG + chr6_1:14419196-14419215 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG - chr6_1:14419227-14419246 Msa0876440:five_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG - chr6_1:14419384-14419403 Msa0876440:five_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr6_1:14415738-14415757 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr6_1:14415850-14415869 Msa0876440:three_prime_UTR 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr6_1:14415966-14415985 Msa0876440:three_prime_UTR 30.0%
CTACATAAACAGGTTATACA+TGG + chr6_1:14416430-14416449 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr6_1:14416440-14416459 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr6_1:14416509-14416528 Msa0876440:intron 30.0%
AATCATACATTGAGGTGATT+AGG + chr6_1:14416557-14416576 None:intergenic 30.0%
CTGAAATACCTACAAAGAAA+TGG + chr6_1:14416601-14416620 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr6_1:14416792-14416811 Msa0876440:intron 30.0%
TTATCTCTAACAGCAAGATA+AGG + chr6_1:14417272-14417291 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG + chr6_1:14417305-14417324 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG - chr6_1:14417385-14417404 Msa0876440:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG - chr6_1:14417539-14417558 Msa0876440:intron 30.0%
TTCGAGTGACAAATGAAAAT+AGG + chr6_1:14417574-14417593 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG + chr6_1:14417653-14417672 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG + chr6_1:14417663-14417682 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG - chr6_1:14417789-14417808 Msa0876440:intron 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG - chr6_1:14417790-14417809 Msa0876440:intron 30.0%
ATGCTAAATACACTTGAACA+CGG - chr6_1:14417840-14417859 Msa0876440:intron 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG - chr6_1:14417864-14417883 Msa0876440:intron 30.0%
GAGAAATGTTGATCAATTAG+GGG - chr6_1:14418348-14418367 Msa0876440:intron 30.0%
TATTGATGAGAATATATGGC+AGG - chr6_1:14418662-14418681 Msa0876440:five_prime_UTR 30.0%
ATAAAACAACCTAACCAGAA+AGG + chr6_1:14418715-14418734 None:intergenic 30.0%
TTCTCATTAAATTGACGAGA+TGG + chr6_1:14418972-14418991 None:intergenic 30.0%
! TTTTAGTCTAACTCTTAGCT+TGG - chr6_1:14419267-14419286 Msa0876440:five_prime_UTR 30.0%
TTGGATATTGTAACAATGAC+AGG - chr6_1:14419403-14419422 Msa0876440:five_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG + chr6_1:14415391-14415410 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG + chr6_1:14415498-14415517 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG + chr6_1:14415556-14415575 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG - chr6_1:14416054-14416073 Msa0876440:intron 35.0%
AGAGATCAGACTTCTTAGTT+GGG + chr6_1:14416124-14416143 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr6_1:14416246-14416265 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr6_1:14416565-14416584 None:intergenic 35.0%
CTTTATCGCCATTTCTTTGT+AGG - chr6_1:14416590-14416609 Msa0876440:intron 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr6_1:14416752-14416771 Msa0876440:intron 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr6_1:14416823-14416842 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr6_1:14416850-14416869 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr6_1:14416854-14416873 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr6_1:14416853-14416872 Msa0876440:intron 35.0%
! ACACGGATTTTGCATTTTAG+TGG - chr6_1:14417857-14417876 Msa0876440:intron 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG - chr6_1:14417865-14417884 Msa0876440:intron 35.0%
TTCTCATTAAATTGCCGAGA+TGG + chr6_1:14418947-14418966 None:intergenic 35.0%
AATCTCACACTAAGCTGAAT+TGG + chr6_1:14419104-14419123 None:intergenic 35.0%
!!! GCTTAGTGTGAGATTAGTTT+TGG - chr6_1:14419109-14419128 Msa0876440:five_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG - chr6_1:14419132-14419151 Msa0876440:five_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG - chr6_1:14419139-14419158 Msa0876440:five_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG - chr6_1:14419294-14419313 Msa0876440:five_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG + chr6_1:14415392-14415411 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG + chr6_1:14415442-14415461 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG + chr6_1:14415483-14415502 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG + chr6_1:14415492-14415511 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG - chr6_1:14415616-14415635 Msa0876440:three_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG + chr6_1:14415628-14415647 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr6_1:14415699-14415718 Msa0876440:three_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr6_1:14415748-14415767 Msa0876440:three_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG + chr6_1:14415955-14415974 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr6_1:14416045-14416064 Msa0876440:intron 40.0%
GAGAGATCAGACTTCTTAGT+TGG + chr6_1:14416125-14416144 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr6_1:14416154-14416173 Msa0876440:three_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr6_1:14416161-14416180 Msa0876440:three_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr6_1:14416271-14416290 Msa0876440:three_prime_UTR 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr6_1:14416317-14416336 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr6_1:14416318-14416337 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr6_1:14416738-14416757 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr6_1:14416735-14416754 Msa0876440:intron 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr6_1:14416767-14416786 Msa0876440:intron 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr6_1:14416778-14416797 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr6_1:14416846-14416865 Msa0876440:intron 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr6_1:14416858-14416877 Msa0876440:intron 40.0%
TTAATTATGGCACAGGAACC+AGG + chr6_1:14417613-14417632 None:intergenic 40.0%
CGTTCCTTATCTATGAAGCA+CGG - chr6_1:14418094-14418113 Msa0876440:CDS 40.0%
GTATCCGTGCTTCATAGATA+AGG + chr6_1:14418101-14418120 None:intergenic 40.0%
TACTTCGACACTCTTACGAT+CGG - chr6_1:14418210-14418229 Msa0876440:intron 40.0%
TTTGTCTGAGTTGTCCGATA+CGG + chr6_1:14418265-14418284 None:intergenic 40.0%
ATAGGAGACTTGTGTTTCAG+CGG + chr6_1:14418627-14418646 None:intergenic 40.0%
AACAACCTAACCAGAAAGGA+AGG + chr6_1:14418711-14418730 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG - chr6_1:14418841-14418860 Msa0876440:intron 40.0%
ACTCCCATAGATTGTCTCAA+GGG - chr6_1:14418842-14418861 Msa0876440:intron 40.0%
ATCCCCTTGAGACAATCTAT+GGG + chr6_1:14418848-14418867 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG + chr6_1:14418849-14418868 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG - chr6_1:14418930-14418949 Msa0876440:intron 40.0%
TCATTAAATTGCCGAGATGG+AGG + chr6_1:14418944-14418963 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG - chr6_1:14418984-14419003 Msa0876440:intron 40.0%
TCTGATGTATAAGCTCCTGA+TGG + chr6_1:14419015-14419034 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG + chr6_1:14415400-14415419 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG + chr6_1:14415401-14415420 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG + chr6_1:14415417-14415436 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG + chr6_1:14415418-14415437 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG + chr6_1:14415437-14415456 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG + chr6_1:14415570-14415589 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG + chr6_1:14415571-14415590 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG - chr6_1:14415574-14415593 Msa0876440:three_prime_UTR 45.0%
AAAGGTAACCGAATTCGCGA+AGG - chr6_1:14415634-14415653 Msa0876440:three_prime_UTR 45.0%
TTCTTCCTCCTTCGCGAATT+CGG + chr6_1:14415645-14415664 None:intergenic 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr6_1:14415762-14415781 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr6_1:14416031-14416050 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr6_1:14416043-14416062 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr6_1:14416044-14416063 Msa0876440:intron 45.0%
CTTCTTAGTTGGGAGATCGA+AGG + chr6_1:14416114-14416133 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr6_1:14416199-14416218 Msa0876440:three_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr6_1:14416297-14416316 Msa0876440:intron 45.0%
CATACCGCGAGAAAGTTGAA+GGG + chr6_1:14416390-14416409 None:intergenic 45.0%
ACATACCGCGAGAAAGTTGA+AGG + chr6_1:14416391-14416410 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr6_1:14416520-14416539 Msa0876440:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr6_1:14416542-14416561 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr6_1:14416766-14416785 Msa0876440:intron 45.0%
GCATCTGAGGGGAAACTAAA+TGG - chr6_1:14417519-14417538 Msa0876440:intron 45.0%
CTGAGGGGAAACTAAATGGT+TGG - chr6_1:14417523-14417542 Msa0876440:intron 45.0%
TATGAAGCACGGATACCTCT+AGG - chr6_1:14418105-14418124 Msa0876440:CDS 45.0%
ACTCTTGTATCTATGGTGCG+CGG + chr6_1:14418316-14418335 None:intergenic 45.0%
AAGAAAACGCATGTGAGCGT+TGG - chr6_1:14418556-14418575 Msa0876440:five_prime_UTR 45.0%
GGACTTTGCTAAGACTTGAG+AGG - chr6_1:14418683-14418702 Msa0876440:five_prime_UTR 45.0%
TTGAGAGGATCCTTCCTTTC+TGG - chr6_1:14418698-14418717 Msa0876440:five_prime_UTR 45.0%
!! AGGATCCTTCCTTTCTGGTT+AGG - chr6_1:14418703-14418722 Msa0876440:five_prime_UTR 45.0%
GGCGAAAAGAACGAAGACAT+AGG - chr6_1:14418798-14418817 Msa0876440:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG - chr6_1:14418843-14418862 Msa0876440:intron 45.0%
TTAAATTGCCGAGATGGAGG+TGG + chr6_1:14418941-14418960 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG - chr6_1:14419145-14419164 Msa0876440:five_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG - chr6_1:14419148-14419167 Msa0876440:five_prime_UTR 45.0%
!!! GGTTTTTGGAGTAGGTGGTT+CGG - chr6_1:14419153-14419172 Msa0876440:five_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG + chr6_1:14415387-14415406 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG + chr6_1:14415388-14415407 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG + chr6_1:14415407-14415426 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG + chr6_1:14415408-14415427 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG + chr6_1:14415416-14415435 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr6_1:14415759-14415778 Msa0876440:three_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr6_1:14415906-14415925 Msa0876440:three_prime_UTR 50.0%
GCCGGAATAGCAAATCTCGT+TGG - chr6_1:14415951-14415970 Msa0876440:three_prime_UTR 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr6_1:14416106-14416125 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr6_1:14416167-14416186 Msa0876440:three_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr6_1:14416197-14416216 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr6_1:14416198-14416217 Msa0876440:three_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr6_1:14416200-14416219 Msa0876440:three_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr6_1:14416521-14416540 Msa0876440:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr6_1:14416541-14416560 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr6_1:14416691-14416710 Msa0876440:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr6_1:14416694-14416713 Msa0876440:intron 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr6_1:14416705-14416724 Msa0876440:intron 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr6_1:14416808-14416827 Msa0876440:intron 50.0%
ACAGTATCCGACAGCATCTG+AGG - chr6_1:14417506-14417525 Msa0876440:intron 50.0%
CAGTATCCGACAGCATCTGA+GGG - chr6_1:14417507-14417526 Msa0876440:intron 50.0%
AGTATCCGACAGCATCTGAG+GGG - chr6_1:14417508-14417527 Msa0876440:intron 50.0%
AGTTTCCCCTCAGATGCTGT+CGG + chr6_1:14417516-14417535 None:intergenic 50.0%
! CCGGTTCGACACATATCGTA+CGG + chr6_1:14418167-14418186 None:intergenic 50.0%
CCGTACGATATGTGTCGAAC+CGG - chr6_1:14418164-14418183 Msa0876440:intron 50.0%
AGGAGCTAGGCTAAACCATC+AGG - chr6_1:14418997-14419016 Msa0876440:intron 50.0%
! CTCTTAGCTTGGCAGTGCAT+AGG - chr6_1:14419278-14419297 Msa0876440:five_prime_UTR 50.0%
GGTAACCGAATTCGCGAAGG+AGG - chr6_1:14415637-14415656 Msa0876440:three_prime_UTR 55.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr6_1:14415724-14415743 Msa0876440:three_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG + chr6_1:14415947-14415966 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG + chr6_1:14415950-14415969 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr6_1:14416214-14416233 None:intergenic 55.0%
CGCTCCCTTCAACTTTCTCG+CGG - chr6_1:14416383-14416402 Msa0876440:intron 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr6_1:14416525-14416544 Msa0876440:intron 55.0%
!! GCACGGATACCTCTAGGACT+AGG - chr6_1:14418111-14418130 Msa0876440:intron 55.0%
!! CTCTAGGACTAGGCATGTCG+CGG - chr6_1:14418121-14418140 Msa0876440:intron 55.0%
!! GTACGGTGTCATACGCGTGT+CGG + chr6_1:14418150-14418169 None:intergenic 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr6_1:14415909-14415928 Msa0876440:three_prime_UTR 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr6_1:14416175-14416194 None:intergenic 60.0%
CGCGACATGCCTAGTCCTAG+AGG + chr6_1:14418123-14418142 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG + chr6_1:14415943-14415962 None:intergenic 65.0%
TATTCCGGCGAGGTGGCCGG+TGG + chr6_1:14415940-14415959 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG + chr6_1:14415923-14415942 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG - chr6_1:14415921-14415940 Msa0876440:three_prime_UTR 80.0%
! GCCACCACCGGCCACCTCGC+CGG - chr6_1:14415933-14415952 Msa0876440:three_prime_UTR 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG + chr6_1:14415937-14415956 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 14415347 14419470 14415347 ID=Msa0876440;Name=Msa0876440
chr6_1 mRNA 14415347 14419470 14415347 ID=Msa0876440-mRNA-1;Parent=Msa0876440;Name=Msa0876440-mRNA-1;_AED=0.22;_eAED=0.22;_QI=722|1|1|1|0.5|0.42|7|900|108
chr6_1 exon 14415347 14416018 14415347 ID=Msa0876440-mRNA-1:exon:4188;Parent=Msa0876440-mRNA-1
chr6_1 exon 14416093 14416297 14416093 ID=Msa0876440-mRNA-1:exon:4187;Parent=Msa0876440-mRNA-1
chr6_1 exon 14417257 14417321 14417257 ID=Msa0876440-mRNA-1:exon:4186;Parent=Msa0876440-mRNA-1
chr6_1 exon 14417806 14417854 14417806 ID=Msa0876440-mRNA-1:exon:4185;Parent=Msa0876440-mRNA-1
chr6_1 exon 14417943 14418124 14417943 ID=Msa0876440-mRNA-1:exon:4184;Parent=Msa0876440-mRNA-1
chr6_1 exon 14418413 14418754 14418413 ID=Msa0876440-mRNA-1:exon:4183;Parent=Msa0876440-mRNA-1
chr6_1 exon 14419037 14419470 14419037 ID=Msa0876440-mRNA-1:exon:4182;Parent=Msa0876440-mRNA-1
chr6_1 five_prime_UTR 14419037 14419470 14419037 ID=Msa0876440-mRNA-1:five_prime_utr;Parent=Msa0876440-mRNA-1
chr6_1 five_prime_UTR 14418467 14418754 14418467 ID=Msa0876440-mRNA-1:five_prime_utr;Parent=Msa0876440-mRNA-1
chr6_1 CDS 14418413 14418466 14418413 ID=Msa0876440-mRNA-1:cds;Parent=Msa0876440-mRNA-1
chr6_1 CDS 14417943 14418124 14417943 ID=Msa0876440-mRNA-1:cds;Parent=Msa0876440-mRNA-1
chr6_1 CDS 14417806 14417854 14417806 ID=Msa0876440-mRNA-1:cds;Parent=Msa0876440-mRNA-1
chr6_1 CDS 14417280 14417321 14417280 ID=Msa0876440-mRNA-1:cds;Parent=Msa0876440-mRNA-1
chr6_1 three_prime_UTR 14417257 14417279 14417257 ID=Msa0876440-mRNA-1:three_prime_utr;Parent=Msa0876440-mRNA-1
chr6_1 three_prime_UTR 14416093 14416297 14416093 ID=Msa0876440-mRNA-1:three_prime_utr;Parent=Msa0876440-mRNA-1
chr6_1 three_prime_UTR 14415347 14416018 14415347 ID=Msa0876440-mRNA-1:three_prime_utr;Parent=Msa0876440-mRNA-1
Gene Sequence

>Msa0876440

ATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTTCAACTTTCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCCGACAGCATCTGAGGGGAAACTAA
Protein sequence

>Msa0876440

MHSFSMLSSSGAPFNFLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSIRQHLRGN*