AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1221040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221040 MtrunA17_Chr1g0209681 77.372 137 7 2 1 114 1 136 2.90e-60 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221040 AT1G57600.1 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa1221040 AT1G57600.2 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa1221040 AT1G57600.3 46.479 142 52 2 1 119 1 141 7.83e-35 126

Find 101 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTCCTTCGCGAATT+TGG 0.160807 8_1:+84490051 None:intergenic
TTGAGAGGATCCGTCCTTTC+TGG 0.187696 8_1:-84487004 Msa1221040:three_prime_UTR
CAAGTAGATATTTATTTAAA+AGG 0.207136 8_1:-84487101 Msa1221040:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 8_1:-84488950 Msa1221040:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 8_1:-84488163 Msa1221040:intron
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 8_1:+84489377 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 8_1:-84489494 Msa1221040:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 8_1:-84488967 Msa1221040:CDS
GCTTAGTCTGAGATTAGTTT+TGG 0.281512 8_1:-84486581 Msa1221040:three_prime_UTR
GAGATCAGACTTCTTAGTTT+GGG 0.303634 8_1:+84489572 None:intergenic
AGAGATCAGACTTCTTAGTT+TGG 0.310750 8_1:+84489571 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 8_1:+84486844 None:intergenic
ATAAAATTATAAGTCTAAAT+AGG 0.331713 8_1:+84487060 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 8_1:-84490119 Msa1221040:five_prime_UTR
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 8_1:-84486633 Msa1221040:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 8_1:-84486306 Msa1221040:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 8_1:-84488183 Msa1221040:exon
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 8_1:+84488927 None:intergenic
AATCTCAGACTAAGCTGAAT+TGG 0.361937 8_1:+84486589 None:intergenic
ATTTGGTTACCTTTGCTGTT+TGG 0.369402 8_1:+84490068 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 8_1:+84490125 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 8_1:-84489493 Msa1221040:five_prime_UTR
AGGATCCGTCCTTTCTGGTT+AGG 0.383361 8_1:-84486999 Msa1221040:intron
TACAAAATCATAGAATTATA+AGG 0.389269 8_1:+84486370 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 8_1:+84490126 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 8_1:+84490296 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 8_1:+84489449 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 8_1:+84490295 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 8_1:+84490259 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 8_1:-84486287 Msa1221040:three_prime_UTR
ACAATTTAATGAGAAGTGAT+AGG 0.441583 8_1:-84486713 Msa1221040:three_prime_UTR
TTGGGAGATCGAAGGAAGAC+AGG 0.456175 8_1:+84489590 None:intergenic
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 8_1:+84490288 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 8_1:-84488856 Msa1221040:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 8_1:+84489934 None:intergenic
ATGCTCCATGCAAATAAGAT+AGG 0.474131 8_1:+84488851 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 8_1:-84488894 Msa1221040:CDS
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 8_1:+84490278 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 8_1:+84490213 None:intergenic
AAATGTAAGTAAAGGAGATA+GGG 0.493237 8_1:-84486632 Msa1221040:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 8_1:-84486558 Msa1221040:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 8_1:+84488967 None:intergenic
AGGAGCTAGGCTAAACCATC+AGG 0.501207 8_1:-84486693 Msa1221040:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 8_1:-84489531 Msa1221040:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 8_1:+84489378 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 8_1:+84486746 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 8_1:+84490254 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 8_1:+84490140 None:intergenic
TTCTCATTAAATTGTCGAGA+TGG 0.524264 8_1:+84486721 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 8_1:-84488936 Msa1221040:CDS
TATTGATGAGAATATATGGC+AGG 0.526440 8_1:-84487040 Msa1221040:three_prime_UTR
CTCTTGCGATTCCACGATTG+GGG 0.530968 8_1:-84489492 Msa1221040:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 8_1:-84488910 Msa1221040:CDS
AAGAAATTCCACCTCCATCT+CGG 0.534799 8_1:-84486760 Msa1221040:three_prime_UTR
AAGAAAACGCATGTGAGCGT+TGG 0.536923 8_1:-84487146 Msa1221040:three_prime_UTR
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 8_1:+84490279 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 8_1:+84490289 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 8_1:+84486752 None:intergenic
CTCTTAGCTTGGCAGTGCAT+AGG 0.552112 8_1:-84486412 Msa1221040:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 8_1:+84486678 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 8_1:-84488935 Msa1221040:CDS
ATAAATTGTTCGATTGAGAA+CGG 0.563409 8_1:+84490250 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 8_1:+84490328 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 8_1:-84488179 Msa1221040:exon
ACAGTATCCGACAGCATCTG+AGG 0.568896 8_1:-84488196 Msa1221040:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 8_1:-84486706 Msa1221040:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 8_1:-84488849 Msa1221040:intron
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 8_1:-84489969 Msa1221040:five_prime_UTR
TTCTTAGTTTGGGAGATCGA+AGG 0.582704 8_1:+84489582 None:intergenic
TCATTAAATTGCCGAGATGG+AGG 0.583358 8_1:+84486749 None:intergenic
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 8_1:+84489498 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 8_1:-84489538 Msa1221040:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 8_1:-84486849 Msa1221040:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 8_1:+84489481 None:intergenic
CAGTATCCGACAGCATCTGA+GGG 0.590060 8_1:-84488195 Msa1221040:CDS
AGTTTCCCCTCAGATGCTGT+CGG 0.590938 8_1:+84488189 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 8_1:+84490280 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 8_1:-84486848 Msa1221040:three_prime_UTR
ATAAAACAACCTAACCAGAA+AGG 0.600525 8_1:+84486990 None:intergenic
AACAACCTAACCAGAAAGGA+CGG 0.604687 8_1:+84486994 None:intergenic
CATCATCAGGCGCTCCCTCG+CGG 0.610543 8_1:-84489319 Msa1221040:intron
TCAAACCTGGAAAACGGACG+CGG 0.612446 8_1:-84489525 Msa1221040:five_prime_UTR
GGACTTTGCTAAGACTTGAG+AGG 0.614168 8_1:-84487019 Msa1221040:three_prime_UTR
ATAGGAGACTTGTGTTTCAG+CGG 0.616380 8_1:+84487078 None:intergenic
CTCCCATAGATTGTCTCAAG+GGG 0.617721 8_1:-84486847 Msa1221040:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 8_1:-84486892 Msa1221040:three_prime_UTR
ATCCCCTTGAGACAATCTAT+GGG 0.622438 8_1:+84486845 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 8_1:-84489994 Msa1221040:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 8_1:-84489008 Msa1221040:CDS
TGAATCGAACCAAACAGCAA+AGG 0.643380 8_1:-84490077 Msa1221040:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 8_1:+84490329 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 8_1:+84490204 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 8_1:+84490198 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 8_1:-84489421 Msa1221040:exon
AAAGGTAACCAAATTCGCGA+AGG 0.655991 8_1:-84490059 Msa1221040:five_prime_UTR
AGAAAACAAAAGCCAGACAA+CGG 0.663004 8_1:+84488882 None:intergenic
AAAGAAGAAACATACCGCGA+GGG 0.665018 8_1:+84489305 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 8_1:+84488855 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 8_1:-84489395 Msa1221040:CDS
AGGATGTCTCAGATGCTCAG+TGG 0.679281 8_1:-84489011 Msa1221040:CDS
AGTATCCGACAGCATCTGAG+GGG 0.723113 8_1:-84488194 Msa1221040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAAAGTTAATTTTTTTTT+AGG + chr8_1:84486255-84486274 None:intergenic 10.0%
!! AGAAAAATAAAACTTAAATA+TGG + chr8_1:84488532-84488551 None:intergenic 10.0%
!! ATAAAATTATAAGTCTAAAT+AGG + chr8_1:84489551-84489570 None:intergenic 10.0%
!!! ATTTGTTTTTTATTTATCGT+CGG - chr8_1:84487387-84487406 Msa1221040:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG - chr8_1:84487896-84487915 Msa1221040:intron 15.0%
!! AAACAATGAAAATTAATCTT+TGG + chr8_1:84488260-84488279 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG - chr8_1:84488259-84488278 Msa1221040:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG + chr8_1:84488633-84488652 None:intergenic 15.0%
!! GATAATAGATGAATAAATAA+AGG - chr8_1:84489318-84489337 Msa1221040:intron 15.0%
!! CAAGTAGATATTTATTTAAA+AGG - chr8_1:84489507-84489526 Msa1221040:five_prime_UTR 15.0%
!!! ATTTTATTGATGAGAATATA+TGG - chr8_1:84489564-84489583 Msa1221040:five_prime_UTR 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG - chr8_1:84489695-84489714 Msa1221040:intron 15.0%
!! TACAAAATCATAGAATTATA+AGG + chr8_1:84490241-84490260 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG - chr8_1:84490293-84490312 Msa1221040:five_prime_UTR 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr8_1:84486801-84486820 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG - chr8_1:84487954-84487973 Msa1221040:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG - chr8_1:84488053-84488072 Msa1221040:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG + chr8_1:84488341-84488360 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG - chr8_1:84488340-84488359 Msa1221040:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG - chr8_1:84488666-84488685 Msa1221040:intron 20.0%
!!! ATACAATTATTCATTTGTTG+AGG + chr8_1:84488916-84488935 None:intergenic 20.0%
!! ATATGAATAGTATATGATGT+TGG - chr8_1:84488949-84488968 Msa1221040:CDS 20.0%
!! TATGAATAGTATATGATGTT+GGG - chr8_1:84488950-84488969 Msa1221040:CDS 20.0%
!!! GTTCTTTAATTTTTACAAGT+TGG - chr8_1:84488972-84488991 Msa1221040:CDS 20.0%
!! AAAAACAATTATTATGACAC+CGG + chr8_1:84489092-84489111 None:intergenic 20.0%
!! AAATAACACACAAATTCATT+AGG + chr8_1:84489691-84489710 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG - chr8_1:84489968-84489987 Msa1221040:five_prime_UTR 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG - chr8_1:84490124-84490143 Msa1221040:five_prime_UTR 20.0%
! ATAAATTGTTCGATTGAGAA+CGG + chr8_1:84486361-84486380 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG - chr8_1:84486708-84486727 Msa1221040:three_prime_UTR 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr8_1:84486816-84486835 Msa1221040:three_prime_UTR 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr8_1:84486982-84487001 Msa1221040:intron 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr8_1:84487276-84487295 Msa1221040:intron 25.0%
! CTGAAATACTTACAAAGAAA+TGG + chr8_1:84487507-84487526 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG + chr8_1:84487577-84487596 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr8_1:84487577-84487596 Msa1221040:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG + chr8_1:84488156-84488175 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG + chr8_1:84488248-84488267 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG - chr8_1:84488372-84488391 Msa1221040:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG - chr8_1:84488498-84488517 Msa1221040:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG + chr8_1:84488526-84488545 None:intergenic 25.0%
! AGCATTATATATGCTCTAAT+TGG - chr8_1:84488881-84488900 Msa1221040:CDS 25.0%
! TATTTGTGTTATATAGGTGT+CGG + chr8_1:84489145-84489164 None:intergenic 25.0%
! ATATGAAACTCTTGTATCTA+TGG + chr8_1:84489229-84489248 None:intergenic 25.0%
! TTGAGAAATGTTGATCAATT+AGG - chr8_1:84489252-84489271 Msa1221040:intron 25.0%
! TGAGAAATGTTGATCAATTA+GGG - chr8_1:84489253-84489272 Msa1221040:intron 25.0%
!!! TTTTTTTTGTTGTGTAGTGT+AGG - chr8_1:84489404-84489423 Msa1221040:CDS 25.0%
!!! TTTTTTACTATTTCAGCAAC+AGG + chr8_1:84489444-84489463 None:intergenic 25.0%
! ACAATTTAATGAGAAGTGAT+AGG - chr8_1:84489895-84489914 Msa1221040:intron 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG - chr8_1:84489975-84489994 Msa1221040:five_prime_UTR 25.0%
! AAATGTAAGTAAAGGAGATA+GGG - chr8_1:84489976-84489995 Msa1221040:five_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG + chr8_1:84490114-84490133 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG - chr8_1:84490145-84490164 Msa1221040:five_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG - chr8_1:84490302-84490321 Msa1221040:five_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr8_1:84486653-84486672 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr8_1:84486765-84486784 Msa1221040:three_prime_UTR 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr8_1:84486881-84486900 Msa1221040:three_prime_UTR 30.0%
CTACATAAACAGGTTATACA+TGG + chr8_1:84487336-84487355 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr8_1:84487346-84487365 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr8_1:84487415-84487434 Msa1221040:intron 30.0%
AATCATACATTGAGGTGATT+AGG + chr8_1:84487463-84487482 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr8_1:84487698-84487717 Msa1221040:intron 30.0%
TCTAAATTGAACAAGTGAGA+AGG + chr8_1:84488211-84488230 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG - chr8_1:84488291-84488310 Msa1221040:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG - chr8_1:84488445-84488464 Msa1221040:intron 30.0%
TTCGAGTGACAAATGAAAAT+AGG + chr8_1:84488480-84488499 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG + chr8_1:84488559-84488578 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG + chr8_1:84488569-84488588 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG - chr8_1:84488695-84488714 Msa1221040:intron 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG - chr8_1:84488696-84488715 Msa1221040:intron 30.0%
ATGCTAAATACACTTGAACA+CGG - chr8_1:84488746-84488765 Msa1221040:intron 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG - chr8_1:84488770-84488789 Msa1221040:intron 30.0%
CGGTTGTATTTGTGTTATAT+AGG + chr8_1:84489151-84489170 None:intergenic 30.0%
GAGAAATGTTGATCAATTAG+GGG - chr8_1:84489254-84489273 Msa1221040:intron 30.0%
TATTGATGAGAATATATGGC+AGG - chr8_1:84489568-84489587 Msa1221040:five_prime_UTR 30.0%
ATAAAACAACCTAACCAGAA+AGG + chr8_1:84489621-84489640 None:intergenic 30.0%
TTCTCATTAAATTGTCGAGA+TGG + chr8_1:84489890-84489909 None:intergenic 30.0%
! TTTTAGTCTAACTCTTAGCT+TGG - chr8_1:84490185-84490204 Msa1221040:five_prime_UTR 30.0%
TTGGATATTGTAACAATGAC+AGG - chr8_1:84490321-84490340 Msa1221040:intron 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG + chr8_1:84486286-84486305 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG + chr8_1:84486413-84486432 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG + chr8_1:84486471-84486490 None:intergenic 35.0%
!! ATTTGGTTACCTTTGCTGTT+TGG + chr8_1:84486543-84486562 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG - chr8_1:84486969-84486988 Msa1221040:intron 35.0%
GAGATCAGACTTCTTAGTTT+GGG + chr8_1:84487039-84487058 None:intergenic 35.0%
AGAGATCAGACTTCTTAGTT+TGG + chr8_1:84487040-84487059 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr8_1:84487162-84487181 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr8_1:84487471-84487490 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr8_1:84487658-84487677 Msa1221040:intron 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr8_1:84487729-84487748 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr8_1:84487756-84487775 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr8_1:84487760-84487779 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr8_1:84487759-84487778 Msa1221040:intron 35.0%
TGATCTCTAACAGCAAGATA+AGG + chr8_1:84488178-84488197 None:intergenic 35.0%
! ACACGGATTTTGCATTTTAG+TGG - chr8_1:84488763-84488782 Msa1221040:intron 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG - chr8_1:84488771-84488790 Msa1221040:intron 35.0%
TAACACAAATACAACCGTGT+CGG - chr8_1:84489154-84489173 Msa1221040:intron 35.0%
TTCTCATTAAATTGCCGAGA+TGG + chr8_1:84489865-84489884 None:intergenic 35.0%
AATCTCAGACTAAGCTGAAT+TGG + chr8_1:84490022-84490041 None:intergenic 35.0%
!!! GCTTAGTCTGAGATTAGTTT+TGG - chr8_1:84490027-84490046 Msa1221040:five_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG - chr8_1:84490050-84490069 Msa1221040:five_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG - chr8_1:84490057-84490076 Msa1221040:five_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG - chr8_1:84490212-84490231 Msa1221040:five_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG + chr8_1:84486287-84486306 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG + chr8_1:84486295-84486314 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG + chr8_1:84486296-84486315 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG + chr8_1:84486302-84486321 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG + chr8_1:84486303-84486322 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG + chr8_1:84486357-84486376 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG + chr8_1:84486398-84486417 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG + chr8_1:84486407-84486426 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG - chr8_1:84486531-84486550 Msa1221040:three_prime_UTR 40.0%
AAAGGTAACCAAATTCGCGA+AGG - chr8_1:84486549-84486568 Msa1221040:three_prime_UTR 40.0%
TTCTTCTTCCTTCGCGAATT+TGG + chr8_1:84486560-84486579 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr8_1:84486614-84486633 Msa1221040:three_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr8_1:84486663-84486682 Msa1221040:three_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG + chr8_1:84486870-84486889 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr8_1:84486960-84486979 Msa1221040:intron 40.0%
! TTCTTAGTTTGGGAGATCGA+AGG + chr8_1:84487029-84487048 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr8_1:84487070-84487089 Msa1221040:three_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr8_1:84487077-84487096 Msa1221040:three_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr8_1:84487187-84487206 Msa1221040:intron 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr8_1:84487233-84487252 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr8_1:84487234-84487253 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG + chr8_1:84487306-84487325 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr8_1:84487644-84487663 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr8_1:84487641-84487660 Msa1221040:intron 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr8_1:84487673-84487692 Msa1221040:intron 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr8_1:84487684-84487703 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr8_1:84487752-84487771 Msa1221040:intron 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr8_1:84487764-84487783 Msa1221040:intron 40.0%
TTAAATATGGCACAGGAACC+AGG + chr8_1:84488519-84488538 None:intergenic 40.0%
CGTTCCTTATCTATGAAGCA+CGG - chr8_1:84489000-84489019 Msa1221040:CDS 40.0%
GTATCCGTGCTTCATAGATA+AGG + chr8_1:84489007-84489026 None:intergenic 40.0%
TACTTCGACACTCTTACGAT+CGG - chr8_1:84489116-84489135 Msa1221040:intron 40.0%
ATAGGAGACTTGTGTTTCAG+CGG + chr8_1:84489533-84489552 None:intergenic 40.0%
AACAACCTAACCAGAAAGGA+CGG + chr8_1:84489617-84489636 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG - chr8_1:84489759-84489778 Msa1221040:intron 40.0%
ACTCCCATAGATTGTCTCAA+GGG - chr8_1:84489760-84489779 Msa1221040:intron 40.0%
ATCCCCTTGAGACAATCTAT+GGG + chr8_1:84489766-84489785 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG + chr8_1:84489767-84489786 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG - chr8_1:84489848-84489867 Msa1221040:intron 40.0%
TCATTAAATTGCCGAGATGG+AGG + chr8_1:84489862-84489881 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG - chr8_1:84489902-84489921 Msa1221040:intron 40.0%
TCTGATGTATAAGCTCCTGA+TGG + chr8_1:84489933-84489952 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG + chr8_1:84486315-84486334 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG + chr8_1:84486316-84486335 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG + chr8_1:84486332-84486351 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG + chr8_1:84486333-84486352 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG + chr8_1:84486352-84486371 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG + chr8_1:84486485-84486504 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG + chr8_1:84486486-84486505 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG - chr8_1:84486489-84486508 Msa1221040:three_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr8_1:84486677-84486696 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr8_1:84486946-84486965 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr8_1:84486958-84486977 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr8_1:84486959-84486978 Msa1221040:intron 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr8_1:84487115-84487134 Msa1221040:three_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr8_1:84487213-84487232 Msa1221040:intron 45.0%
GAAAGAAGAAACATACCGCG+AGG + chr8_1:84487307-84487326 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr8_1:84487426-84487445 Msa1221040:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr8_1:84487448-84487467 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr8_1:84487672-84487691 Msa1221040:intron 45.0%
GCATCTGAGGGGAAACTAAA+TGG - chr8_1:84488425-84488444 Msa1221040:intron 45.0%
CTGAGGGGAAACTAAATGGT+TGG - chr8_1:84488429-84488448 Msa1221040:intron 45.0%
TATGAAGCACGGATACCTCT+AGG - chr8_1:84489011-84489030 Msa1221040:CDS 45.0%
! CCGGTTCGACACATATCTTA+CGG + chr8_1:84489073-84489092 None:intergenic 45.0%
CCGTAAGATATGTGTCGAAC+CGG - chr8_1:84489070-84489089 Msa1221040:intron 45.0%
TTTGTCTGAGTTGTCCGACA+CGG + chr8_1:84489171-84489190 None:intergenic 45.0%
ACTCTTGTATCTATGGTGCG+CGG + chr8_1:84489222-84489241 None:intergenic 45.0%
AAGAAAACGCATGTGAGCGT+TGG - chr8_1:84489462-84489481 Msa1221040:five_prime_UTR 45.0%
GGACTTTGCTAAGACTTGAG+AGG - chr8_1:84489589-84489608 Msa1221040:five_prime_UTR 45.0%
GGCGAAAAGAACGAAGACAT+AGG - chr8_1:84489716-84489735 Msa1221040:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG - chr8_1:84489761-84489780 Msa1221040:intron 45.0%
TTAAATTGCCGAGATGGAGG+TGG + chr8_1:84489859-84489878 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG - chr8_1:84490063-84490082 Msa1221040:five_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG - chr8_1:84490066-84490085 Msa1221040:five_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG + chr8_1:84486282-84486301 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG + chr8_1:84486283-84486302 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG + chr8_1:84486322-84486341 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG + chr8_1:84486323-84486342 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG + chr8_1:84486331-84486350 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr8_1:84486674-84486693 Msa1221040:three_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr8_1:84486821-84486840 Msa1221040:three_prime_UTR 50.0%
GCCGGAATAGCAAATCTCGT+TGG - chr8_1:84486866-84486885 Msa1221040:three_prime_UTR 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr8_1:84487021-84487040 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr8_1:84487083-84487102 Msa1221040:three_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr8_1:84487113-84487132 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr8_1:84487114-84487133 Msa1221040:three_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr8_1:84487116-84487135 Msa1221040:three_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr8_1:84487427-84487446 Msa1221040:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr8_1:84487447-84487466 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr8_1:84487597-84487616 Msa1221040:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr8_1:84487600-84487619 Msa1221040:intron 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr8_1:84487611-84487630 Msa1221040:intron 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr8_1:84487714-84487733 Msa1221040:intron 50.0%
ACAGTATCCGACAGCATCTG+AGG - chr8_1:84488412-84488431 Msa1221040:intron 50.0%
CAGTATCCGACAGCATCTGA+GGG - chr8_1:84488413-84488432 Msa1221040:intron 50.0%
AGTATCCGACAGCATCTGAG+GGG - chr8_1:84488414-84488433 Msa1221040:intron 50.0%
AGTTTCCCCTCAGATGCTGT+CGG + chr8_1:84488422-84488441 None:intergenic 50.0%
!! TTACGGTGTCATACGCGTGT+CGG + chr8_1:84489056-84489075 None:intergenic 50.0%
TTGAGAGGATCCGTCCTTTC+TGG - chr8_1:84489604-84489623 Msa1221040:five_prime_UTR 50.0%
!! AGGATCCGTCCTTTCTGGTT+AGG - chr8_1:84489609-84489628 Msa1221040:five_prime_UTR 50.0%
AGGAGCTAGGCTAAACCATC+AGG - chr8_1:84489915-84489934 Msa1221040:intron 50.0%
! CTCTTAGCTTGGCAGTGCAT+AGG - chr8_1:84490196-84490215 Msa1221040:five_prime_UTR 50.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr8_1:84486639-84486658 Msa1221040:three_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG + chr8_1:84486862-84486881 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG + chr8_1:84486865-84486884 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr8_1:84487130-84487149 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr8_1:84487431-84487450 Msa1221040:intron 55.0%
!! GCACGGATACCTCTAGGACT+AGG - chr8_1:84489017-84489036 Msa1221040:intron 55.0%
!! CTCTAGGACTAGGCATGTCG+CGG - chr8_1:84489027-84489046 Msa1221040:intron 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr8_1:84486824-84486843 Msa1221040:three_prime_UTR 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr8_1:84487091-84487110 None:intergenic 60.0%
CGCGACATGCCTAGTCCTAG+AGG + chr8_1:84489029-84489048 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG + chr8_1:84486858-84486877 None:intergenic 65.0%
CATCATCAGGCGCTCCCTCG+CGG - chr8_1:84487289-84487308 Msa1221040:intron 65.0%
TATTCCGGCGAGGTGGCCGG+TGG + chr8_1:84486855-84486874 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG + chr8_1:84486838-84486857 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG - chr8_1:84486836-84486855 Msa1221040:three_prime_UTR 80.0%
! GCCACCACCGGCCACCTCGC+CGG - chr8_1:84486848-84486867 Msa1221040:three_prime_UTR 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG + chr8_1:84486852-84486871 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84486242 84490388 84486242 ID=Msa1221040;Name=Msa1221040
chr8_1 mRNA 84486242 84490388 84486242 ID=Msa1221040-mRNA-1;Parent=Msa1221040;Name=Msa1221040-mRNA-1;_AED=0.22;_eAED=0.22;_QI=656|1|1|1|0.57|0.25|8|900|127
chr8_1 exon 84486242 84486913 84486242 ID=Msa1221040-mRNA-1:exon:30360;Parent=Msa1221040-mRNA-1
chr8_1 exon 84487000 84487204 84487000 ID=Msa1221040-mRNA-1:exon:30359;Parent=Msa1221040-mRNA-1
chr8_1 exon 84488164 84488228 84488164 ID=Msa1221040-mRNA-1:exon:30358;Parent=Msa1221040-mRNA-1
chr8_1 exon 84488713 84488761 84488713 ID=Msa1221040-mRNA-1:exon:30357;Parent=Msa1221040-mRNA-1
chr8_1 exon 84488850 84489031 84488850 ID=Msa1221040-mRNA-1:exon:30356;Parent=Msa1221040-mRNA-1
chr8_1 exon 84489320 84489652 84489320 ID=Msa1221040-mRNA-1:exon:30355;Parent=Msa1221040-mRNA-1
chr8_1 exon 84489935 84490321 84489935 ID=Msa1221040-mRNA-1:exon:30354;Parent=Msa1221040-mRNA-1
chr8_1 exon 84490342 84490388 84490342 ID=Msa1221040-mRNA-1:exon:30353;Parent=Msa1221040-mRNA-1
chr8_1 five_prime_UTR 84490342 84490388 84490342 ID=Msa1221040-mRNA-1:five_prime_utr;Parent=Msa1221040-mRNA-1
chr8_1 five_prime_UTR 84489935 84490321 84489935 ID=Msa1221040-mRNA-1:five_prime_utr;Parent=Msa1221040-mRNA-1
chr8_1 five_prime_UTR 84489431 84489652 84489431 ID=Msa1221040-mRNA-1:five_prime_utr;Parent=Msa1221040-mRNA-1
chr8_1 CDS 84489320 84489430 84489320 ID=Msa1221040-mRNA-1:cds;Parent=Msa1221040-mRNA-1
chr8_1 CDS 84488850 84489031 84488850 ID=Msa1221040-mRNA-1:cds;Parent=Msa1221040-mRNA-1
chr8_1 CDS 84488713 84488761 84488713 ID=Msa1221040-mRNA-1:cds;Parent=Msa1221040-mRNA-1
chr8_1 CDS 84488187 84488228 84488187 ID=Msa1221040-mRNA-1:cds;Parent=Msa1221040-mRNA-1
chr8_1 three_prime_UTR 84488164 84488186 84488164 ID=Msa1221040-mRNA-1:three_prime_utr;Parent=Msa1221040-mRNA-1
chr8_1 three_prime_UTR 84487000 84487204 84487000 ID=Msa1221040-mRNA-1:three_prime_utr;Parent=Msa1221040-mRNA-1
chr8_1 three_prime_UTR 84486242 84486913 84486242 ID=Msa1221040-mRNA-1:three_prime_utr;Parent=Msa1221040-mRNA-1
Gene Sequence

>Msa1221040

ATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCCGACAGCATCTGAGGGGAAACTAA
Protein sequence

>Msa1221040

MNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSIRQHLRGN*