Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0469480 | XP_013461269.1 | 97.170 | 106 | 3 | 0 | 1 | 106 | 94 | 199 | 3.86e-69 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0469480 | A0A072UZT9 | 97.170 | 106 | 3 | 0 | 1 | 106 | 94 | 199 | 1.84e-69 | 215 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010150 | Msa0469480 | 0.804147 | 2.483384e-49 | -8.615850e-47 |
| Msa0374030 | Msa0469480 | 0.834658 | 2.639512e-56 | -8.615850e-47 |
| Msa0374040 | Msa0469480 | 0.814522 | 1.476006e-51 | -8.615850e-47 |
| Msa0444250 | Msa0469480 | 0.800429 | 1.446965e-48 | -8.615850e-47 |
| Msa0469450 | Msa0469480 | 0.834829 | 2.390590e-56 | -8.615850e-47 |
| Msa0469460 | Msa0469480 | 0.850959 | 1.211948e-60 | -8.615850e-47 |
| Msa0469480 | Msa0515130 | 0.803240 | 3.830442e-49 | -8.615850e-47 |
| Msa0469480 | Msa1069350 | 0.801652 | 8.138524e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0469480 | MtrunA17_Chr3g0124061 | 93.396 | 106 | 7 | 0 | 1 | 106 | 94 | 199 | 5.71e-71 | 210 |
| Msa0469480 | MtrunA17_Chr3g0124111 | 86.792 | 106 | 14 | 0 | 1 | 106 | 94 | 199 | 8.73e-67 | 199 |
| Msa0469480 | MtrunA17_Chr3g0124141 | 86.792 | 106 | 14 | 0 | 1 | 106 | 94 | 199 | 4.95e-65 | 195 |
| Msa0469480 | MtrunA17_Chr3g0124151 | 84.906 | 106 | 16 | 0 | 1 | 106 | 1 | 106 | 2.02e-63 | 187 |
| Msa0469480 | MtrunA17_Chr3g0124091 | 84.906 | 106 | 15 | 1 | 1 | 106 | 96 | 200 | 6.36e-61 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGAGAAGGTTGAATAAT+TGG | 0.248389 | 3_3:-71267641 | Msa0469480:CDS |
| TTAATTTCCACTTCCACTTC+TGG | 0.251842 | 3_3:+71267702 | None:intergenic |
| TGCGCTTGGTGCAGCTTTAG+AGG | 0.317354 | 3_3:-71267550 | Msa0469480:CDS |
| AAAATGGTTGAAAATGGATT+TGG | 0.402081 | 3_3:-71267763 | Msa0469480:CDS |
| CGCAAATAAATCACAATCTT+CGG | 0.408596 | 3_3:+71267569 | None:intergenic |
| TCCACTTCCACTTCTGGTGT+TGG | 0.429577 | 3_3:+71267708 | None:intergenic |
| GATTCAAGAATCACTTTCCA+TGG | 0.437928 | 3_3:+71267804 | None:intergenic |
| AGTGACTTCGGTTGCTGAGA+TGG | 0.471131 | 3_3:-71267673 | Msa0469480:CDS |
| TTGGCAAAGGTGAGATTCCA+TGG | 0.486019 | 3_3:-71267821 | Msa0469480:CDS |
| GATTGTGATTTATTTGCGCT+TGG | 0.539399 | 3_3:-71267564 | Msa0469480:CDS |
| GCCAACACCAGAAGTGGAAG+TGG | 0.540390 | 3_3:-71267709 | Msa0469480:CDS |
| TGGAAAAGAAGAATAAGAGA+AGG | 0.547734 | 3_3:-71267653 | Msa0469480:CDS |
| AATTAAAATAAGAGTGACTT+CGG | 0.548743 | 3_3:-71267685 | Msa0469480:CDS |
| TTGAAAATGGATTTGGTGAG+TGG | 0.556088 | 3_3:-71267756 | Msa0469480:CDS |
| TTGAATAATTGGAATGAGTG+TGG | 0.570637 | 3_3:-71267630 | Msa0469480:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.583125 | 3_3:-71267793 | Msa0469480:CDS |
| TATGTTGCCAACACCAGAAG+TGG | 0.592676 | 3_3:-71267715 | Msa0469480:CDS |
| GGGTCACAACTTATTGGCAA+AGG | 0.622442 | 3_3:-71267834 | Msa0469480:CDS |
| ATGGTCATGATCATAATGCT+TGG | 0.627084 | 3_3:-71267599 | Msa0469480:CDS |
| TAAGGCAAAGGAAGATGTGG+GGG | 0.660678 | 3_3:-71267854 | None:intergenic |
| CTAAGGCAAAGGAAGATGTG+GGG | 0.689053 | 3_3:-71267855 | None:intergenic |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.732454 | 3_3:-71267739 | Msa0469480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAAATAAGAGTGACTT+CGG | - | chr3_3:71267697-71267716 | Msa0469480:CDS | 20.0% |
| ! | AAAACATGGAGCTAAAAAAA+TGG | - | chr3_3:71267603-71267622 | Msa0469480:CDS | 25.0% |
| ! | GCTAAAAAAATGGTTGAAAA+TGG | - | chr3_3:71267613-71267632 | Msa0469480:CDS | 25.0% |
| !! | AAAATGGTTGAAAATGGATT+TGG | - | chr3_3:71267619-71267638 | Msa0469480:CDS | 25.0% |
| ! | ATAAGAGAAGGTTGAATAAT+TGG | - | chr3_3:71267741-71267760 | Msa0469480:CDS | 25.0% |
| GATTCTTGAATCACAAAACA+TGG | - | chr3_3:71267589-71267608 | Msa0469480:CDS | 30.0% | |
| TGGAAAAGAAGAATAAGAGA+AGG | - | chr3_3:71267729-71267748 | Msa0469480:CDS | 30.0% | |
| TTGAATAATTGGAATGAGTG+TGG | - | chr3_3:71267752-71267771 | Msa0469480:CDS | 30.0% | |
| CGCAAATAAATCACAATCTT+CGG | + | chr3_3:71267816-71267835 | None:intergenic | 30.0% | |
| GATTCAAGAATCACTTTCCA+TGG | + | chr3_3:71267581-71267600 | None:intergenic | 35.0% | |
| !! | TTGAAAATGGATTTGGTGAG+TGG | - | chr3_3:71267626-71267645 | Msa0469480:CDS | 35.0% |
| TTAATTTCCACTTCCACTTC+TGG | + | chr3_3:71267683-71267702 | None:intergenic | 35.0% | |
| AATGAGTGTGGATGCAAAAA+TGG | - | chr3_3:71267764-71267783 | Msa0469480:CDS | 35.0% | |
| ATGGTCATGATCATAATGCT+TGG | - | chr3_3:71267783-71267802 | Msa0469480:CDS | 35.0% | |
| GATTGTGATTTATTTGCGCT+TGG | - | chr3_3:71267818-71267837 | Msa0469480:CDS | 35.0% | |
| ATGTGGGGGTCACAACTTAT+TGG | - | chr3_3:71267542-71267561 | Msa0469480:CDS | 45.0% | |
| GGGTCACAACTTATTGGCAA+AGG | - | chr3_3:71267548-71267567 | Msa0469480:CDS | 45.0% | |
| TTGGCAAAGGTGAGATTCCA+TGG | - | chr3_3:71267561-71267580 | Msa0469480:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | chr3_3:71267643-71267662 | Msa0469480:CDS | 45.0% | |
| TATGTTGCCAACACCAGAAG+TGG | - | chr3_3:71267667-71267686 | Msa0469480:CDS | 45.0% | |
| TCCACTTCCACTTCTGGTGT+TGG | + | chr3_3:71267677-71267696 | None:intergenic | 50.0% | |
| AGTGACTTCGGTTGCTGAGA+TGG | - | chr3_3:71267709-71267728 | Msa0469480:CDS | 50.0% | |
| GCCAACACCAGAAGTGGAAG+TGG | - | chr3_3:71267673-71267692 | Msa0469480:CDS | 55.0% | |
| !! | TGCGCTTGGTGCAGCTTTAG+AGG | - | chr3_3:71267832-71267851 | Msa0469480:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 71267542 | 71267862 | 71267542 | ID=Msa0469480;Name=Msa0469480 |
| chr3_3 | mRNA | 71267542 | 71267862 | 71267542 | ID=Msa0469480-mRNA-1;Parent=Msa0469480;Name=Msa0469480-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|106 |
| chr3_3 | exon | 71267542 | 71267862 | 71267542 | ID=Msa0469480-mRNA-1:exon:17528;Parent=Msa0469480-mRNA-1 |
| chr3_3 | CDS | 71267542 | 71267862 | 71267542 | ID=Msa0469480-mRNA-1:cds;Parent=Msa0469480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
