AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469450 MtrunA17_Chr3g0124061 93.467 199 13 0 1 199 1 199 2.46e-140 389
Msa0469450 MtrunA17_Chr3g0124111 87.437 199 25 0 1 199 1 199 3.57e-131 366
Msa0469450 MtrunA17_Chr3g0124141 85.427 199 29 0 1 199 1 199 2.61e-125 351
Msa0469450 MtrunA17_Chr3g0124091 84.577 201 28 2 1 199 1 200 2.62e-121 341
Msa0469450 MtrunA17_Chr3g0124151 83.019 106 18 0 94 199 1 106 1.10e-61 187
Msa0469450 MtrunA17_Chr3g0124131 78.431 51 11 0 1 51 1 51 4.41e-24 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469450 AT5G65030.1 30.964 197 124 3 11 199 10 202 4.26e-26 99.8
Msa0469450 AT5G10210.1 31.633 196 112 7 15 199 13 197 5.12e-24 94.4

Find 34 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACCAAATCAGTACCATTT+TGG 0.165786 3_3:-71261974 Msa0469450:CDS
ATAAGAGTAGGTTGAATAAT+TGG 0.258714 3_3:-71261659 Msa0469450:CDS
TTAATCTCCACTTCTACTTC+CGG 0.353807 3_3:+71261720 None:intergenic
TGCGCTTAGCGCAGCTTTGG+AGG 0.362485 3_3:-71261568 Msa0469450:CDS
TTAAATGACTCGTCCCAAAA+TGG 0.387620 3_3:+71261960 None:intergenic
GCTCCAACACTAGGCTTTGT+TGG 0.406615 3_3:+71261895 None:intergenic
AGTGGCTACGGTTCATGAAA+TGG 0.409468 3_3:-71261691 Msa0469450:CDS
GTCCCAAAATGGTACTGATT+TGG 0.410434 3_3:+71261971 None:intergenic
ATTTGCGCTTAGCGCAGCTT+TGG 0.427353 3_3:-71261571 Msa0469450:CDS
GATTCTAGAATCACTTTCCA+TGG 0.440184 3_3:+71261822 None:intergenic
GCAAGGCTGTATCTTCCAAC+AGG 0.447411 3_3:-71262035 Msa0469450:CDS
TGCACCAAGCGCGATGATCA+TGG 0.461361 3_3:+71261613 None:intergenic
TTGGAAAAGGTGAGATTCCA+TGG 0.470388 3_3:-71261839 Msa0469450:CDS
AAAGCCTAGTGTTGGAGCTA+AGG 0.499139 3_3:-71261890 Msa0469450:CDS
TTTGCCTTAGCTCCAACACT+AGG 0.507447 3_3:+71261886 None:intergenic
CAACTGAAGTTTGCATATGC+AGG 0.509989 3_3:+71262119 None:intergenic
GGGTCACAACTTATTGGAAA+AGG 0.536727 3_3:-71261852 Msa0469450:CDS
AGTGGAGATTAAAATAAGAG+TGG 0.548596 3_3:-71261709 Msa0469450:CDS
AATGAGTGTGGATGCAAGAA+TGG 0.549203 3_3:-71261636 Msa0469450:CDS
ATGGCCATGATCATCGCGCT+TGG 0.550412 3_3:-71261617 Msa0469450:CDS
GATTAAAATAAGAGTGGCTA+CGG 0.551977 3_3:-71261703 Msa0469450:CDS
GCTAAGGCAAAGGAAGATGT+GGG 0.557016 3_3:-71261874 Msa0469450:CDS
AGCTAAGGCAAAGGAAGATG+TGG 0.560256 3_3:-71261875 Msa0469450:CDS
TAACCAACAAAGCCTAGTGT+TGG 0.564525 3_3:-71261898 Msa0469450:CDS
TTGAAGATATATTTAGTGAG+TGG 0.575240 3_3:-71261774 Msa0469450:CDS
TTGAATAATTGGAATGAGTG+TGG 0.579695 3_3:-71261648 Msa0469450:CDS
GATTCTAGAATCACAAAACA+TGG 0.587863 3_3:-71261811 Msa0469450:CDS
TAGTGTTGGAGCTAAGGCAA+AGG 0.590191 3_3:-71261884 Msa0469450:CDS
CTCAACACCGGAAGTAGAAG+TGG 0.592858 3_3:-71261727 Msa0469450:CDS
AGAGGAAATGTTCTCAACAC+CGG 0.624098 3_3:-71261739 Msa0469450:CDS
GAGTGGAAGTGATTGCAAAG+AGG 0.644927 3_3:-71261757 Msa0469450:CDS
TAAGGCAAAGGAAGATGTGG+GGG 0.660678 3_3:-71261872 Msa0469450:CDS
AAACGTTGAATTCATCAAGT+CGG 0.660973 3_3:-71262072 Msa0469450:CDS
CTAAGGCAAAGGAAGATGTG+GGG 0.689053 3_3:-71261873 Msa0469450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAACATGGAGTTGAAAAAA+TGG - chr3_3:71261900-71261919 Msa0469450:CDS 25.0%
! TTGAAGATATATTTAGTGAG+TGG - chr3_3:71261923-71261942 Msa0469450:CDS 25.0%
! TGGAAAAGAAAAATAAGAGT+AGG - chr3_3:71262026-71262045 Msa0469450:CDS 25.0%
!! ATAAGAGTAGGTTGAATAAT+TGG - chr3_3:71262038-71262057 Msa0469450:CDS 25.0%
AAACGTTGAATTCATCAAGT+CGG - chr3_3:71261625-71261644 Msa0469450:CDS 30.0%
!!! ATACTTTTGTTGTTTCCTGT+TGG + chr3_3:71261680-71261699 None:intergenic 30.0%
! TACCAAATCAGTACCATTTT+GGG - chr3_3:71261724-71261743 Msa0469450:CDS 30.0%
! AAGATTTTCCAAGAATTCCT+GGG + chr3_3:71261780-71261799 None:intergenic 30.0%
! TAAGATTTTCCAAGAATTCC+TGG + chr3_3:71261781-71261800 None:intergenic 30.0%
!! GATTCTAGAATCACAAAACA+TGG - chr3_3:71261886-71261905 Msa0469450:CDS 30.0%
AGTGGAGATTAAAATAAGAG+TGG - chr3_3:71261988-71262007 Msa0469450:CDS 30.0%
GATTAAAATAAGAGTGGCTA+CGG - chr3_3:71261994-71262013 Msa0469450:CDS 30.0%
TTGAATAATTGGAATGAGTG+TGG - chr3_3:71262049-71262068 Msa0469450:CDS 30.0%
! CTACCAAATCAGTACCATTT+TGG - chr3_3:71261723-71261742 Msa0469450:CDS 35.0%
TTAAATGACTCGTCCCAAAA+TGG + chr3_3:71261740-71261759 None:intergenic 35.0%
! AGATTTTCCAAGAATTCCTG+GGG + chr3_3:71261779-71261798 None:intergenic 35.0%
!! GATTCTAGAATCACTTTCCA+TGG + chr3_3:71261878-71261897 None:intergenic 35.0%
TTAATCTCCACTTCTACTTC+CGG + chr3_3:71261980-71261999 None:intergenic 35.0%
CAACTGAAGTTTGCATATGC+AGG + chr3_3:71261581-71261600 None:intergenic 40.0%
CGGCAAAAAACATGTTTGCA+AGG - chr3_3:71261645-71261664 Msa0469450:CDS 40.0%
!! GTCCCAAAATGGTACTGATT+TGG + chr3_3:71261729-71261748 None:intergenic 40.0%
! GATTTTCCAAGAATTCCTGG+GGG + chr3_3:71261778-71261797 None:intergenic 40.0%
TAACCAACAAAGCCTAGTGT+TGG - chr3_3:71261799-71261818 Msa0469450:CDS 40.0%
GGGTCACAACTTATTGGAAA+AGG - chr3_3:71261845-71261864 Msa0469450:CDS 40.0%
TTGGAAAAGGTGAGATTCCA+TGG - chr3_3:71261858-71261877 Msa0469450:CDS 40.0%
AGAGGAAATGTTCTCAACAC+CGG - chr3_3:71261958-71261977 Msa0469450:CDS 40.0%
AATGAGTGTGGATGCAAGAA+TGG - chr3_3:71262061-71262080 Msa0469450:CDS 40.0%
! GCATATGCAGGTGTTTTCGA+TGG + chr3_3:71261569-71261588 None:intergenic 45.0%
! AAAGCCTAGTGTTGGAGCTA+AGG - chr3_3:71261807-71261826 Msa0469450:CDS 45.0%
TTTGCCTTAGCTCCAACACT+AGG + chr3_3:71261814-71261833 None:intergenic 45.0%
! TAGTGTTGGAGCTAAGGCAA+AGG - chr3_3:71261813-71261832 Msa0469450:CDS 45.0%
AGCTAAGGCAAAGGAAGATG+TGG - chr3_3:71261822-71261841 Msa0469450:CDS 45.0%
GCTAAGGCAAAGGAAGATGT+GGG - chr3_3:71261823-71261842 Msa0469450:CDS 45.0%
CTAAGGCAAAGGAAGATGTG+GGG - chr3_3:71261824-71261843 Msa0469450:CDS 45.0%
TAAGGCAAAGGAAGATGTGG+GGG - chr3_3:71261825-71261844 Msa0469450:CDS 45.0%
ATGTGGGGGTCACAACTTAT+TGG - chr3_3:71261839-71261858 Msa0469450:CDS 45.0%
GAGTGGAAGTGATTGCAAAG+AGG - chr3_3:71261940-71261959 Msa0469450:CDS 45.0%
AGTGGCTACGGTTCATGAAA+TGG - chr3_3:71262006-71262025 Msa0469450:CDS 45.0%
GCAAGGCTGTATCTTCCAAC+AGG - chr3_3:71261662-71261681 Msa0469450:CDS 50.0%
TCTAGATTGTTCTTGCCCCC+AGG - chr3_3:71261760-71261779 Msa0469450:CDS 50.0%
TTCTTGCCCCCAGGAATTCT+TGG - chr3_3:71261769-71261788 Msa0469450:CDS 50.0%
! GCTCCAACACTAGGCTTTGT+TGG + chr3_3:71261805-71261824 None:intergenic 50.0%
CTCAACACCGGAAGTAGAAG+TGG - chr3_3:71261970-71261989 Msa0469450:CDS 50.0%
ATTTGCGCTTAGCGCAGCTT+TGG - chr3_3:71262126-71262145 Msa0469450:CDS 50.0%
ATGGCCATGATCATCGCGCT+TGG - chr3_3:71262080-71262099 Msa0469450:CDS 55.0%
TGCACCAAGCGCGATGATCA+TGG + chr3_3:71262087-71262106 None:intergenic 55.0%
! TGCGCTTAGCGCAGCTTTGG+AGG - chr3_3:71262129-71262148 Msa0469450:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71261560 71262159 71261560 ID=Msa0469450;Name=Msa0469450
chr3_3 mRNA 71261560 71262159 71261560 ID=Msa0469450-mRNA-1;Parent=Msa0469450;Name=Msa0469450-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|199
chr3_3 exon 71261560 71262159 71261560 ID=Msa0469450-mRNA-1:exon:17523;Parent=Msa0469450-mRNA-1
chr3_3 CDS 71261560 71262159 71261560 ID=Msa0469450-mRNA-1:cds;Parent=Msa0469450-mRNA-1
Gene Sequence

>Msa0469450

ATGGATCCATCGAAAACACCTGCATATGCAAACTTCAGTTGTGAACTGAGAATAATACAAGCACGAAACGTTGAATTCATCAAGTCGGCAAAAAACATGTTTGCAAGGCTGTATCTTCCAACAGGAAACAACAAAAGTATTCAACTCAACAGCAAAAAAGTTTCTACCAAATCAGTACCATTTTGGGACGAGTCATTTAATCTAGATTGTTCTTGCCCCCAGGAATTCTTGGAAAATCTTAACCAACAAAGCCTAGTGTTGGAGCTAAGGCAAAGGAAGATGTGGGGGTCACAACTTATTGGAAAAGGTGAGATTCCATGGAAAGTGATTCTAGAATCACAAAACATGGAGTTGAAAAAATGGTTGAAGATATATTTAGTGAGTGGAAGTGATTGCAAAGAGGAAATGTTCTCAACACCGGAAGTAGAAGTGGAGATTAAAATAAGAGTGGCTACGGTTCATGAAATGGAAAAGAAAAATAAGAGTAGGTTGAATAATTGGAATGAGTGTGGATGCAAGAATGGCCATGATCATCGCGCTTGGTGCAGCGCTGAAGATTGTGATATATTTGCGCTTAGCGCAGCTTTGGAGGCTTTTTGA

Protein sequence

>Msa0469450

MDPSKTPAYANFSCELRIIQARNVEFIKSAKNMFARLYLPTGNNKSIQLNSKKVSTKSVPFWDESFNLDCSCPQEFLENLNQQSLVLELRQRKMWGSQLIGKGEIPWKVILESQNMELKKWLKIYLVSGSDCKEEMFSTPEVEVEIKIRVATVHEMEKKNKSRLNNWNECGCKNGHDHRAWCSAEDCDIFALSAALEAF*