AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0374030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0374030 MtrunA17_Chr3g0124121 75.758 33 8 0 38 70 1 33 8.39e-11 52.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 3 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAATGACATCTTAGACTT+CGG 0.475221 3_1:+73149857 None:intergenic
ACGAAGTGTCTTTCAACTAC+TGG 0.497425 3_1:-73150027 Msa0374030:CDS
TAACACTGTCATCGTACTGT+TGG 0.576545 3_1:-73149894 Msa0374030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAATATATTTAATGTTT+TGG - chr3_1:73149939-73149958 Msa0374030:CDS 10.0%
!! TATGTATAGATAAAAATTTA+AGG + chr3_1:73150628-73150647 None:intergenic 10.0%
!!! AAAGAATATATTTAATGTTT+TGG - chr3_1:73149939-73149958 Msa0374030:CDS 10.0%
!! TATGTATAGATAAAAATTTA+AGG + chr3_1:73150628-73150647 None:intergenic 10.0%
!!! ATTATTCTAATGTTACTTTT+GGG + chr3_1:73150291-73150310 None:intergenic 15.0%
!! AAATGATATTAACTTTATGA+TGG + chr3_1:73150521-73150540 None:intergenic 15.0%
!! ATCTATACATAGTTTATATA+TGG - chr3_1:73150637-73150656 Msa0374030:intron 15.0%
!!! ATTATTCTAATGTTACTTTT+GGG + chr3_1:73150291-73150310 None:intergenic 15.0%
!! AAATGATATTAACTTTATGA+TGG + chr3_1:73150521-73150540 None:intergenic 15.0%
!! ATCTATACATAGTTTATATA+TGG - chr3_1:73150637-73150656 Msa0374030:intron 15.0%
!!! TTATTCTAATGTTACTTTTG+GGG + chr3_1:73150290-73150309 None:intergenic 20.0%
!!! GATTATTCTAATGTTACTTT+TGG + chr3_1:73150292-73150311 None:intergenic 20.0%
!! AAGTAACATTAGAATAATCA+AGG - chr3_1:73150293-73150312 Msa0374030:intron 20.0%
!!! TTTAAGGCATTTAGTTTTTA+AGG + chr3_1:73150612-73150631 None:intergenic 20.0%
!! ATAATTGTGCAATTTATTGA+AGG - chr3_1:73150967-73150986 Msa0374030:intron 20.0%
!!! TTATTCTAATGTTACTTTTG+GGG + chr3_1:73150290-73150309 None:intergenic 20.0%
!!! GATTATTCTAATGTTACTTT+TGG + chr3_1:73150292-73150311 None:intergenic 20.0%
!! AAGTAACATTAGAATAATCA+AGG - chr3_1:73150293-73150312 Msa0374030:intron 20.0%
!!! TTTAAGGCATTTAGTTTTTA+AGG + chr3_1:73150612-73150631 None:intergenic 20.0%
!! ATAATTGTGCAATTTATTGA+AGG - chr3_1:73150967-73150986 Msa0374030:intron 20.0%
!! TTGTTATTCTGATATGGTAT+TGG - chr3_1:73150222-73150241 Msa0374030:intron 25.0%
! TGTTATTCTGATATGGTATT+GGG - chr3_1:73150223-73150242 Msa0374030:intron 25.0%
! ATATTTGTAAGCTTAATGAG+TGG + chr3_1:73150470-73150489 None:intergenic 25.0%
!!! CCTCAATATTGTTTTAGATA+AGG + chr3_1:73150561-73150580 None:intergenic 25.0%
!! CCTTATCTAAAACAATATTG+AGG - chr3_1:73150558-73150577 Msa0374030:intron 25.0%
!! TTGTTATTCTGATATGGTAT+TGG - chr3_1:73150222-73150241 Msa0374030:intron 25.0%
! TGTTATTCTGATATGGTATT+GGG - chr3_1:73150223-73150242 Msa0374030:intron 25.0%
! ATATTTGTAAGCTTAATGAG+TGG + chr3_1:73150470-73150489 None:intergenic 25.0%
!!! CCTCAATATTGTTTTAGATA+AGG + chr3_1:73150561-73150580 None:intergenic 25.0%
!! CCTTATCTAAAACAATATTG+AGG - chr3_1:73150558-73150577 Msa0374030:intron 25.0%
! CTCTTATTCTCCATTTTCAA+TGG + chr3_1:73149868-73149887 None:intergenic 30.0%
ATGTGATGCATTATATAGTG+TGG - chr3_1:73149981-73150000 Msa0374030:CDS 30.0%
TGCATTATATAGTGTGGAAA+AGG - chr3_1:73149987-73150006 Msa0374030:CDS 30.0%
ATAGTGTGGAAAAGGTAAAT+TGG - chr3_1:73149995-73150014 Msa0374030:CDS 30.0%
! AAAAAGCACAACTGAGATAT+TGG + chr3_1:73150085-73150104 None:intergenic 30.0%
! TGCATGGTTTAAGATTAAGA+TGG + chr3_1:73150123-73150142 None:intergenic 30.0%
!! AAAACCTTTAGAATCTCGAA+AGG - chr3_1:73150178-73150197 Msa0374030:intron 30.0%
! AAAGGCTAGATTTTCCAATT+TGG - chr3_1:73150196-73150215 Msa0374030:intron 30.0%
TCTTAATGCAATCCATAACT+TGG - chr3_1:73150262-73150281 Msa0374030:intron 30.0%
CTTAATGCAATCCATAACTT+GGG - chr3_1:73150263-73150282 Msa0374030:intron 30.0%
TGACTAAAATTCTTGAATGC+TGG - chr3_1:73150325-73150344 Msa0374030:intron 30.0%
TAGATAAGAATTCCAACTGA+GGG + chr3_1:73150382-73150401 None:intergenic 30.0%
TTAGATAAGAATTCCAACTG+AGG + chr3_1:73150383-73150402 None:intergenic 30.0%
ATAGTTTATATATGGCTGTC+AGG - chr3_1:73150645-73150664 Msa0374030:intron 30.0%
TCAATTCACTCTAAGCTAAA+GGG + chr3_1:73150845-73150864 None:intergenic 30.0%
TTCAATTCACTCTAAGCTAA+AGG + chr3_1:73150846-73150865 None:intergenic 30.0%
! TTTAGCTTAGAGTGAATTGA+AGG - chr3_1:73150845-73150864 Msa0374030:intron 30.0%
TTAGAGTGAATTGAAGGAAT+GGG - chr3_1:73150851-73150870 Msa0374030:intron 30.0%
TAGAATGACATCTTAGACTT+CGG + chr3_1:73151176-73151195 None:intergenic 30.0%
! CTCTTATTCTCCATTTTCAA+TGG + chr3_1:73149868-73149887 None:intergenic 30.0%
ATGTGATGCATTATATAGTG+TGG - chr3_1:73149981-73150000 Msa0374030:CDS 30.0%
TGCATTATATAGTGTGGAAA+AGG - chr3_1:73149987-73150006 Msa0374030:CDS 30.0%
ATAGTGTGGAAAAGGTAAAT+TGG - chr3_1:73149995-73150014 Msa0374030:CDS 30.0%
! AAAAAGCACAACTGAGATAT+TGG + chr3_1:73150085-73150104 None:intergenic 30.0%
! TGCATGGTTTAAGATTAAGA+TGG + chr3_1:73150123-73150142 None:intergenic 30.0%
!! AAAACCTTTAGAATCTCGAA+AGG - chr3_1:73150178-73150197 Msa0374030:intron 30.0%
! AAAGGCTAGATTTTCCAATT+TGG - chr3_1:73150196-73150215 Msa0374030:intron 30.0%
TCTTAATGCAATCCATAACT+TGG - chr3_1:73150262-73150281 Msa0374030:intron 30.0%
CTTAATGCAATCCATAACTT+GGG - chr3_1:73150263-73150282 Msa0374030:intron 30.0%
TGACTAAAATTCTTGAATGC+TGG - chr3_1:73150325-73150344 Msa0374030:intron 30.0%
TAGATAAGAATTCCAACTGA+GGG + chr3_1:73150382-73150401 None:intergenic 30.0%
TTAGATAAGAATTCCAACTG+AGG + chr3_1:73150383-73150402 None:intergenic 30.0%
ATAGTTTATATATGGCTGTC+AGG - chr3_1:73150645-73150664 Msa0374030:intron 30.0%
TCAATTCACTCTAAGCTAAA+GGG + chr3_1:73150845-73150864 None:intergenic 30.0%
TTCAATTCACTCTAAGCTAA+AGG + chr3_1:73150846-73150865 None:intergenic 30.0%
! TTTAGCTTAGAGTGAATTGA+AGG - chr3_1:73150845-73150864 Msa0374030:intron 30.0%
TTAGAGTGAATTGAAGGAAT+GGG - chr3_1:73150851-73150870 Msa0374030:intron 30.0%
TAGAATGACATCTTAGACTT+CGG + chr3_1:73151176-73151195 None:intergenic 30.0%
TTCGATGGATCCATTGAAAA+TGG - chr3_1:73149855-73149874 Msa0374030:CDS 35.0%
GTAAATTGGAAATGCTTTCC+AGG - chr3_1:73150009-73150028 Msa0374030:CDS 35.0%
ATACACTATGTTAGTGCTCA+AGG + chr3_1:73150048-73150067 None:intergenic 35.0%
CAGAATAACAAGAGCCAAAT+TGG + chr3_1:73150213-73150232 None:intergenic 35.0%
!! TGGCTCTTGTTATTCTGATA+TGG - chr3_1:73150216-73150235 Msa0374030:intron 35.0%
TGGATTGCATTAAGATACTG+TGG + chr3_1:73150257-73150276 None:intergenic 35.0%
! TTATTGATGGCTGTCAATTG+AGG + chr3_1:73150351-73150370 None:intergenic 35.0%
TCAATTGACAGCCATCAATA+AGG - chr3_1:73150350-73150369 Msa0374030:intron 35.0%
TTGTAAGCTTAATGAGTGGT+AGG + chr3_1:73150466-73150485 None:intergenic 35.0%
! CAATATTGAGGAGCGAATTT+TGG - chr3_1:73150570-73150589 Msa0374030:intron 35.0%
CTTAGAGTGAATTGAAGGAA+TGG - chr3_1:73150850-73150869 Msa0374030:intron 35.0%
TTCGATGGATCCATTGAAAA+TGG - chr3_1:73149855-73149874 Msa0374030:CDS 35.0%
GTAAATTGGAAATGCTTTCC+AGG - chr3_1:73150009-73150028 Msa0374030:CDS 35.0%
ATACACTATGTTAGTGCTCA+AGG + chr3_1:73150048-73150067 None:intergenic 35.0%
CAGAATAACAAGAGCCAAAT+TGG + chr3_1:73150213-73150232 None:intergenic 35.0%
!! TGGCTCTTGTTATTCTGATA+TGG - chr3_1:73150216-73150235 Msa0374030:intron 35.0%
TGGATTGCATTAAGATACTG+TGG + chr3_1:73150257-73150276 None:intergenic 35.0%
! TTATTGATGGCTGTCAATTG+AGG + chr3_1:73150351-73150370 None:intergenic 35.0%
TCAATTGACAGCCATCAATA+AGG - chr3_1:73150350-73150369 Msa0374030:intron 35.0%
TTGTAAGCTTAATGAGTGGT+AGG + chr3_1:73150466-73150485 None:intergenic 35.0%
! CAATATTGAGGAGCGAATTT+TGG - chr3_1:73150570-73150589 Msa0374030:intron 35.0%
CTTAGAGTGAATTGAAGGAA+TGG - chr3_1:73150850-73150869 Msa0374030:intron 35.0%
TTGTAGCTCGAAATAGAGAG+AGG - chr3_1:73149905-73149924 Msa0374030:CDS 40.0%
TCAACACAATCTCATGTGCA+TGG + chr3_1:73150139-73150158 None:intergenic 40.0%
! CTAGCCTTTCGAGATTCTAA+AGG + chr3_1:73150185-73150204 None:intergenic 40.0%
GTTTATATATGGCTGTCAGG+TGG - chr3_1:73150648-73150667 Msa0374030:intron 40.0%
TCAGTCTCTCCAATTCTTAC+TGG + chr3_1:73150737-73150756 None:intergenic 40.0%
AGCAGTGCATACCTTTCTAT+TGG - chr3_1:73150768-73150787 Msa0374030:intron 40.0%
GCAGTGCATACCTTTCTATT+GGG - chr3_1:73150769-73150788 Msa0374030:intron 40.0%
CAGTGCATACCTTTCTATTG+GGG - chr3_1:73150770-73150789 Msa0374030:intron 40.0%
GCGAAGTTTGGATTGATCAT+TGG - chr3_1:73150885-73150904 Msa0374030:intron 40.0%
!! TAAACAACATTGCCGACTTG+GGG + chr3_1:73150930-73150949 None:intergenic 40.0%
!! CTAAACAACATTGCCGACTT+GGG + chr3_1:73150931-73150950 None:intergenic 40.0%
! TCTAAACAACATTGCCGACT+TGG + chr3_1:73150932-73150951 None:intergenic 40.0%
! ACGAAGTGTCTTTCAACTAC+TGG - chr3_1:73151003-73151022 Msa0374030:intron 40.0%
TAACACTGTCATCGTACTGT+TGG - chr3_1:73151136-73151155 Msa0374030:intron 40.0%
TTGTAGCTCGAAATAGAGAG+AGG - chr3_1:73149905-73149924 Msa0374030:CDS 40.0%
TCAACACAATCTCATGTGCA+TGG + chr3_1:73150139-73150158 None:intergenic 40.0%
! CTAGCCTTTCGAGATTCTAA+AGG + chr3_1:73150185-73150204 None:intergenic 40.0%
GTTTATATATGGCTGTCAGG+TGG - chr3_1:73150648-73150667 Msa0374030:intron 40.0%
TCAGTCTCTCCAATTCTTAC+TGG + chr3_1:73150737-73150756 None:intergenic 40.0%
AGCAGTGCATACCTTTCTAT+TGG - chr3_1:73150768-73150787 Msa0374030:intron 40.0%
GCAGTGCATACCTTTCTATT+GGG - chr3_1:73150769-73150788 Msa0374030:intron 40.0%
CAGTGCATACCTTTCTATTG+GGG - chr3_1:73150770-73150789 Msa0374030:intron 40.0%
GCGAAGTTTGGATTGATCAT+TGG - chr3_1:73150885-73150904 Msa0374030:intron 40.0%
!! TAAACAACATTGCCGACTTG+GGG + chr3_1:73150930-73150949 None:intergenic 40.0%
!! CTAAACAACATTGCCGACTT+GGG + chr3_1:73150931-73150950 None:intergenic 40.0%
! TCTAAACAACATTGCCGACT+TGG + chr3_1:73150932-73150951 None:intergenic 40.0%
! ACGAAGTGTCTTTCAACTAC+TGG - chr3_1:73151003-73151022 Msa0374030:intron 40.0%
TAACACTGTCATCGTACTGT+TGG - chr3_1:73151136-73151155 Msa0374030:intron 40.0%
!!! ACTTTTGGGGTCCCAAGTTA+TGG + chr3_1:73150277-73150296 None:intergenic 45.0%
!! GAGGGTTCTAGCCTTATTGA+TGG + chr3_1:73150364-73150383 None:intergenic 45.0%
ATAAGGCTAGAACCCTCAGT+TGG - chr3_1:73150367-73150386 Msa0374030:intron 45.0%
! TTGAGCACTGTAATGCTTGC+AGG + chr3_1:73150713-73150732 None:intergenic 45.0%
GTGCTCAAGCCAGTAAGAAT+TGG - chr3_1:73150725-73150744 Msa0374030:intron 45.0%
GCATACCTTTCTATTGGGGT+TGG - chr3_1:73150774-73150793 Msa0374030:intron 45.0%
GAGATCCAACCCCAATAGAA+AGG + chr3_1:73150782-73150801 None:intergenic 45.0%
GAAGGAATGGGTCTTCATGA+AGG - chr3_1:73150863-73150882 Msa0374030:intron 45.0%
GTCTTCATGAAGGCGAAGTT+TGG - chr3_1:73150873-73150892 Msa0374030:intron 45.0%
!! AAACAACATTGCCGACTTGG+GGG + chr3_1:73150929-73150948 None:intergenic 45.0%
!!! ACTTTTGGGGTCCCAAGTTA+TGG + chr3_1:73150277-73150296 None:intergenic 45.0%
!! GAGGGTTCTAGCCTTATTGA+TGG + chr3_1:73150364-73150383 None:intergenic 45.0%
ATAAGGCTAGAACCCTCAGT+TGG - chr3_1:73150367-73150386 Msa0374030:intron 45.0%
! TTGAGCACTGTAATGCTTGC+AGG + chr3_1:73150713-73150732 None:intergenic 45.0%
GTGCTCAAGCCAGTAAGAAT+TGG - chr3_1:73150725-73150744 Msa0374030:intron 45.0%
GCATACCTTTCTATTGGGGT+TGG - chr3_1:73150774-73150793 Msa0374030:intron 45.0%
GAGATCCAACCCCAATAGAA+AGG + chr3_1:73150782-73150801 None:intergenic 45.0%
GAAGGAATGGGTCTTCATGA+AGG - chr3_1:73150863-73150882 Msa0374030:intron 45.0%
GTCTTCATGAAGGCGAAGTT+TGG - chr3_1:73150873-73150892 Msa0374030:intron 45.0%
!! AAACAACATTGCCGACTTGG+GGG + chr3_1:73150929-73150948 None:intergenic 45.0%
CAAGGATAAGAGCAGCTTCC+TGG + chr3_1:73150030-73150049 None:intergenic 50.0%
AACTGACTGGAGACACTCTC+CGG - chr3_1:73150671-73150690 Msa0374030:intron 50.0%
ACACTCTCCGGTGAAGCAAA+GGG - chr3_1:73150683-73150702 Msa0374030:intron 50.0%
AGGTACTCCCTTTGCTTCAC+CGG + chr3_1:73150693-73150712 None:intergenic 50.0%
CAAAACAGTTGCCCCCAAGT+CGG - chr3_1:73150915-73150934 Msa0374030:intron 50.0%
CAAGGATAAGAGCAGCTTCC+TGG + chr3_1:73150030-73150049 None:intergenic 50.0%
AACTGACTGGAGACACTCTC+CGG - chr3_1:73150671-73150690 Msa0374030:intron 50.0%
ACACTCTCCGGTGAAGCAAA+GGG - chr3_1:73150683-73150702 Msa0374030:intron 50.0%
AGGTACTCCCTTTGCTTCAC+CGG + chr3_1:73150693-73150712 None:intergenic 50.0%
CAAAACAGTTGCCCCCAAGT+CGG - chr3_1:73150915-73150934 Msa0374030:intron 50.0%
GACACTCTCCGGTGAAGCAA+AGG - chr3_1:73150682-73150701 Msa0374030:intron 55.0%
!! GGGGGCAACTGTTTTGGACT+AGG + chr3_1:73150911-73150930 None:intergenic 55.0%
!! CGACTTGGGGGCAACTGTTT+TGG + chr3_1:73150917-73150936 None:intergenic 55.0%
GACACTCTCCGGTGAAGCAA+AGG - chr3_1:73150682-73150701 Msa0374030:intron 55.0%
!! GGGGGCAACTGTTTTGGACT+AGG + chr3_1:73150911-73150930 None:intergenic 55.0%
!! CGACTTGGGGGCAACTGTTT+TGG + chr3_1:73150917-73150936 None:intergenic 55.0%
GGCTGTCAGGTGGAACTGAC+TGG - chr3_1:73150658-73150677 Msa0374030:intron 60.0%
GGCTGTCAGGTGGAACTGAC+TGG - chr3_1:73150658-73150677 Msa0374030:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 73149844 73151208 73149844 ID=Msa0374030;Name=Msa0374030
chr3_1 mRNA 73149844 73151208 73149844 ID=Msa0374030-mRNA-1;Parent=Msa0374030;Name=Msa0374030-mRNA-1;_AED=0.63;_eAED=0.63;_QI=0|0|0|0.5|1|1|2|0|70
chr3_1 exon 73151203 73151208 73151203 ID=Msa0374030-mRNA-1:exon:16629;Parent=Msa0374030-mRNA-1
chr3_1 exon 73149844 73150050 73149844 ID=Msa0374030-mRNA-1:exon:16628;Parent=Msa0374030-mRNA-1
chr3_1 CDS 73151203 73151208 73151203 ID=Msa0374030-mRNA-1:cds;Parent=Msa0374030-mRNA-1
chr3_1 CDS 73149844 73150050 73149844 ID=Msa0374030-mRNA-1:cds;Parent=Msa0374030-mRNA-1
Gene Sequence

>Msa0374030

ATGCAGAACGAAGTGTCTTTCAACTACTGGCGTACACGATATCTTTCTACTTCTATAAAAGAACGACACAGTGTCAATTTAGTGAAGCAACTTCGTCGACGAAAGCTAAAGATGTTTGTCTACTTCGATAAAAGAAGTGCTAACACTGTCATCGTACTGTTGGAGAGTCTTCATTTACCGAAGTCTAAGATGTCATTCTACTTCGATAAATAA

Protein sequence

>Msa0374030

MQNEVSFNYWRTRYLSTSIKERHSVNLVKQLRRRKLKMFVYFDKRSANTVIVLLESLHLPKSKMSFYFDK*