AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0469460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469460 MtrunA17_Chr3g0124061 89.447 199 21 0 1 199 1 199 5.29e-134 373
Msa0469460 MtrunA17_Chr3g0124111 87.940 199 24 0 1 199 1 199 6.17e-133 370
Msa0469460 MtrunA17_Chr3g0124141 88.945 199 22 0 1 199 1 199 2.93e-131 366
Msa0469460 MtrunA17_Chr3g0124091 89.055 201 19 2 1 199 1 200 8.08e-129 360
Msa0469460 MtrunA17_Chr3g0124151 85.849 106 15 0 94 199 1 106 4.03e-63 191
Msa0469460 MtrunA17_Chr3g0124131 80.000 50 10 0 1 50 1 50 5.13e-24 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0469460 AT5G65030.1 30.612 196 113 6 11 187 10 201 8.23e-24 94.0
Msa0469460 AT5G10210.1 30.769 195 109 7 15 196 13 194 2.41e-23 92.4

Find 43 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACCAAATCAGTGCCCTTT+TGG 0.235029 3_3:-71265228 Msa0469460:CDS
CTCATGCCCTCAAGAGTTCT+TGG 0.272286 3_3:-71265182 Msa0469460:CDS
ATAAGAGGAGAATGAATAAT+TGG 0.280000 3_3:-71264913 Msa0469460:CDS
TTAATCTCCACTTCCACTTC+TGG 0.281665 3_3:+71264974 None:intergenic
ATGTTGGGGTCACAACTTAT+TGG 0.325437 3_3:-71265112 Msa0469460:CDS
CCACTAGGCTTTGTGTGTTA+AGG 0.346972 3_3:+71265155 None:intergenic
TGTTGGGGTCACAACTTATT+GGG 0.372157 3_3:-71265111 Msa0469460:CDS
ATTTGCGCTTGGTGCAGCTT+TGG 0.382032 3_3:-71264825 Msa0469460:CDS
ATCCCAAAAGGGCACTGATT+TGG 0.392569 3_3:+71265225 None:intergenic
TGCGCTTGGTGCAGCTTTGG+AGG 0.432752 3_3:-71264822 Msa0469460:CDS
ATTAAAAGACTCATCCCAAA+AGG 0.445229 3_3:+71265213 None:intergenic
GCAAGGCTGTATCTTCCAAC+AGG 0.447411 3_3:-71265289 Msa0469460:CDS
GATTCAAGAATCACCTTCCA+TGG 0.467787 3_3:+71265076 None:intergenic
AAAATGGTTGAAGATGGATT+TGG 0.470551 3_3:-71265035 Msa0469460:CDS
AGTAGCTTCGGTTGCTGAGA+TGG 0.471131 3_3:-71264945 Msa0469460:CDS
GATTAAAATAAAAGTAGCTT+CGG 0.474275 3_3:-71264957 Msa0469460:CDS
CTAAGGCAGAGGAAGATGTT+GGG 0.479580 3_3:-71265127 Msa0469460:CDS
CCAAAAGGGCACTGATTTGG+TGG 0.481239 3_3:+71265228 None:intergenic
AGTGGAAGTGATTGCAAAGA+GGG 0.484026 3_3:-71265010 Msa0469460:CDS
TTGGGAAAGGTGAGATTCCA+TGG 0.488562 3_3:-71265093 Msa0469460:CDS
AGATGGAGAAGCAAAATAAG+AGG 0.509817 3_3:-71264928 Msa0469460:CDS
CAACTGAAGTTTGCATATGC+AGG 0.509989 3_3:+71265373 None:intergenic
GCTAAGGCAGAGGAAGATGT+TGG 0.514746 3_3:-71265128 Msa0469460:CDS
TCTGCCTTAGCTCCACCACT+AGG 0.532067 3_3:+71265140 None:intergenic
ACGGCCATGACCATCGTGCT+TGG 0.538014 3_3:-71264871 Msa0469460:CDS
AAAGCCTAGTGGTGGAGCTA+AGG 0.543884 3_3:-71265144 Msa0469460:CDS
TTAAAAGACTCATCCCAAAA+GGG 0.557578 3_3:+71265214 None:intergenic
ATGAATAATTGGAATGAGTG+TGG 0.557798 3_3:-71264902 Msa0469460:CDS
GGGTCACAACTTATTGGGAA+AGG 0.564555 3_3:-71265106 Msa0469460:CDS
AGCACCAAGCACGATGGTCA+TGG 0.566327 3_3:+71264867 None:intergenic
CATAACGCCAGAAGTGGAAG+TGG 0.570407 3_3:-71264981 Msa0469460:CDS
TAGTGGTGGAGCTAAGGCAG+AGG 0.576496 3_3:-71265138 Msa0469460:CDS
GATTGTGATATATTTGCGCT+TGG 0.580894 3_3:-71264836 Msa0469460:CDS
TATGTTCATAACGCCAGAAG+TGG 0.583659 3_3:-71264987 Msa0469460:CDS
GATTCTTGAATCACAAAACA+TGG 0.589945 3_3:-71265065 Msa0469460:CDS
TTGAAGATGGATTTGGTGAG+TGG 0.598106 3_3:-71265028 Msa0469460:CDS
GAAAGGTGAGATTCCATGGA+AGG 0.610143 3_3:-71265089 Msa0469460:CDS
TAAGGCAGAGGAAGATGTTG+GGG 0.620231 3_3:-71265126 Msa0469460:CDS
TAACACACAAAGCCTAGTGG+TGG 0.644101 3_3:-71265152 Msa0469460:CDS
AAACGTTGAATTCATCAAGT+CGG 0.660973 3_3:-71265326 Msa0469460:CDS
CAGCGCAGCACCAAGCACGA+TGG 0.681591 3_3:+71264861 None:intergenic
CCTTAACACACAAAGCCTAG+TGG 0.691369 3_3:-71265155 Msa0469460:CDS
GAGTGGAAGTGATTGCAAAG+AGG 0.703550 3_3:-71265011 Msa0469460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACATGGAGTTAAAAAAA+TGG - chr3_3:71265154-71265173 Msa0469460:CDS 20.0%
!! GATTAAAATAAAAGTAGCTT+CGG - chr3_3:71265248-71265267 Msa0469460:CDS 20.0%
! GTTAAAAAAATGGTTGAAGA+TGG - chr3_3:71265164-71265183 Msa0469460:CDS 25.0%
! ATAAGAGGAGAATGAATAAT+TGG - chr3_3:71265292-71265311 Msa0469460:CDS 25.0%
AAACGTTGAATTCATCAAGT+CGG - chr3_3:71264879-71264898 Msa0469460:CDS 30.0%
!! ATTCTTTTCTTGTTTCCTGT+TGG + chr3_3:71264934-71264953 None:intergenic 30.0%
TTAAAAGACTCATCCCAAAA+GGG + chr3_3:71264994-71265013 None:intergenic 30.0%
ATTAAAAGACTCATCCCAAA+AGG + chr3_3:71264995-71265014 None:intergenic 30.0%
GATTCTTGAATCACAAAACA+TGG - chr3_3:71265140-71265159 Msa0469460:CDS 30.0%
! AAAATGGTTGAAGATGGATT+TGG - chr3_3:71265170-71265189 Msa0469460:CDS 30.0%
ATGAATAATTGGAATGAGTG+TGG - chr3_3:71265303-71265322 Msa0469460:CDS 30.0%
AGATGGAGAAGCAAAATAAG+AGG - chr3_3:71265277-71265296 Msa0469460:CDS 35.0%
AATGAGTGTGGATGCAAAAA+CGG - chr3_3:71265315-71265334 Msa0469460:CDS 35.0%
GATTGTGATATATTTGCGCT+TGG - chr3_3:71265369-71265388 Msa0469460:CDS 35.0%
CAACTGAAGTTTGCATATGC+AGG + chr3_3:71264835-71264854 None:intergenic 40.0%
CGGCAAAAAACATGTTTGCA+AGG - chr3_3:71264899-71264918 Msa0469460:CDS 40.0%
! GGTTTTCCAAGAACTCTTGA+GGG + chr3_3:71265032-71265051 None:intergenic 40.0%
! AGGTTTTCCAAGAACTCTTG+AGG + chr3_3:71265033-71265052 None:intergenic 40.0%
ATGTTGGGGTCACAACTTAT+TGG - chr3_3:71265093-71265112 Msa0469460:CDS 40.0%
TGTTGGGGTCACAACTTATT+GGG - chr3_3:71265094-71265113 Msa0469460:CDS 40.0%
GATTCAAGAATCACCTTCCA+TGG + chr3_3:71265132-71265151 None:intergenic 40.0%
!! TTGAAGATGGATTTGGTGAG+TGG - chr3_3:71265177-71265196 Msa0469460:CDS 40.0%
AGTGGAAGTGATTGCAAAGA+GGG - chr3_3:71265195-71265214 Msa0469460:CDS 40.0%
TATGTTCATAACGCCAGAAG+TGG - chr3_3:71265218-71265237 Msa0469460:CDS 40.0%
TTAATCTCCACTTCCACTTC+TGG + chr3_3:71265234-71265253 None:intergenic 40.0%
! GCATATGCAGGTGTTTTCGA+TGG + chr3_3:71264823-71264842 None:intergenic 45.0%
! CACCAAATCAGTGCCCTTTT+GGG - chr3_3:71264978-71264997 Msa0469460:CDS 45.0%
!! ATCCCAAAAGGGCACTGATT+TGG + chr3_3:71264983-71265002 None:intergenic 45.0%
! CCACTAGGCTTTGTGTGTTA+AGG + chr3_3:71265053-71265072 None:intergenic 45.0%
CCTTAACACACAAAGCCTAG+TGG - chr3_3:71265050-71265069 Msa0469460:CDS 45.0%
TAACACACAAAGCCTAGTGG+TGG - chr3_3:71265053-71265072 Msa0469460:CDS 45.0%
CTAAGGCAGAGGAAGATGTT+GGG - chr3_3:71265078-71265097 Msa0469460:CDS 45.0%
TAAGGCAGAGGAAGATGTTG+GGG - chr3_3:71265079-71265098 Msa0469460:CDS 45.0%
GGGTCACAACTTATTGGGAA+AGG - chr3_3:71265099-71265118 Msa0469460:CDS 45.0%
TTGGGAAAGGTGAGATTCCA+TGG - chr3_3:71265112-71265131 Msa0469460:CDS 45.0%
GAAAGGTGAGATTCCATGGA+AGG - chr3_3:71265116-71265135 Msa0469460:CDS 45.0%
GAGTGGAAGTGATTGCAAAG+AGG - chr3_3:71265194-71265213 Msa0469460:CDS 45.0%
GCAAGGCTGTATCTTCCAAC+AGG - chr3_3:71264916-71264935 Msa0469460:CDS 50.0%
!! CCAAAAGGGCACTGATTTGG+TGG + chr3_3:71264980-71264999 None:intergenic 50.0%
! CCACCAAATCAGTGCCCTTT+TGG - chr3_3:71264977-71264996 Msa0469460:CDS 50.0%
CTCATGCCCTCAAGAGTTCT+TGG - chr3_3:71265023-71265042 Msa0469460:CDS 50.0%
! AAAGCCTAGTGGTGGAGCTA+AGG - chr3_3:71265061-71265080 Msa0469460:CDS 50.0%
GCTAAGGCAGAGGAAGATGT+TGG - chr3_3:71265077-71265096 Msa0469460:CDS 50.0%
CATAACGCCAGAAGTGGAAG+TGG - chr3_3:71265224-71265243 Msa0469460:CDS 50.0%
AGTAGCTTCGGTTGCTGAGA+TGG - chr3_3:71265260-71265279 Msa0469460:CDS 50.0%
!! ATTTGCGCTTGGTGCAGCTT+TGG - chr3_3:71265380-71265399 Msa0469460:CDS 50.0%
TCTGCCTTAGCTCCACCACT+AGG + chr3_3:71265068-71265087 None:intergenic 55.0%
! TAGTGGTGGAGCTAAGGCAG+AGG - chr3_3:71265067-71265086 Msa0469460:CDS 55.0%
AGCACCAAGCACGATGGTCA+TGG + chr3_3:71265341-71265360 None:intergenic 55.0%
ACGGCCATGACCATCGTGCT+TGG - chr3_3:71265334-71265353 Msa0469460:CDS 60.0%
!! TGCGCTTGGTGCAGCTTTGG+AGG - chr3_3:71265383-71265402 Msa0469460:CDS 60.0%
CAGCGCAGCACCAAGCACGA+TGG + chr3_3:71265347-71265366 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 71264814 71265413 71264814 ID=Msa0469460;Name=Msa0469460
chr3_3 mRNA 71264814 71265413 71264814 ID=Msa0469460-mRNA-1;Parent=Msa0469460;Name=Msa0469460-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|199
chr3_3 exon 71264814 71265413 71264814 ID=Msa0469460-mRNA-1:exon:17524;Parent=Msa0469460-mRNA-1
chr3_3 CDS 71264814 71265413 71264814 ID=Msa0469460-mRNA-1:cds;Parent=Msa0469460-mRNA-1
Gene Sequence

>Msa0469460

ATGGATCCATCGAAAACACCTGCATATGCAAACTTCAGTTGTGAACTGAGAATAATACAAGCACGAAACGTTGAATTCATCAAGTCGGCAAAAAACATGTTTGCAAGGCTGTATCTTCCAACAGGAAACAAGAAAAGAATTCAACTAAACAGCAAAAATGTTTCCACCAAATCAGTGCCCTTTTGGGATGAGTCTTTTAATCTAGACTGCTCATGCCCTCAAGAGTTCTTGGAAAACCTTAACACACAAAGCCTAGTGGTGGAGCTAAGGCAGAGGAAGATGTTGGGGTCACAACTTATTGGGAAAGGTGAGATTCCATGGAAGGTGATTCTTGAATCACAAAACATGGAGTTAAAAAAATGGTTGAAGATGGATTTGGTGAGTGGAAGTGATTGCAAAGAGGGTATGTTCATAACGCCAGAAGTGGAAGTGGAGATTAAAATAAAAGTAGCTTCGGTTGCTGAGATGGAGAAGCAAAATAAGAGGAGAATGAATAATTGGAATGAGTGTGGATGCAAAAACGGCCATGACCATCGTGCTTGGTGCTGCGCTGAAGATTGTGATATATTTGCGCTTGGTGCAGCTTTGGAGGCGTTTTAA

Protein sequence

>Msa0469460

MDPSKTPAYANFSCELRIIQARNVEFIKSAKNMFARLYLPTGNKKRIQLNSKNVSTKSVPFWDESFNLDCSCPQEFLENLNTQSLVVELRQRKMLGSQLIGKGEIPWKVILESQNMELKKWLKMDLVSGSDCKEGMFITPEVEVEIKIKVASVAEMEKQNKRRMNNWNECGCKNGHDHRAWCCAEDCDIFALGAALEAF*