AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0474330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474330 MtrunA17_Chr3g0131071 91.186 590 31 7 1 586 1 573 0.0 1003
Msa0474330 MtrunA17_Chr8g0385291 70.876 491 119 10 112 586 21 503 0.0 668
Msa0474330 MtrunA17_Chr4g0014901 68.475 387 117 4 199 582 208 592 0.0 546
Msa0474330 MtrunA17_Chr4g0014911 63.081 344 120 2 244 582 121 462 1.94e-158 461
Msa0474330 MtrunA17_Chr2g0293201 54.913 346 146 6 245 583 124 466 6.99e-134 399
Msa0474330 MtrunA17_Chr4g0004981 51.045 335 155 6 245 574 107 437 7.14e-114 347
Msa0474330 MtrunA17_Chr1g0183881 41.379 348 191 7 240 581 124 464 4.85e-91 288
Msa0474330 MtrunA17_Chr7g0243541 41.618 346 148 9 247 586 100 397 8.15e-83 265
Msa0474330 MtrunA17_Chr3g0131581 30.476 210 132 4 260 469 67 262 1.10e-22 98.2
Msa0474330 MtrunA17_Chr8g0385681 30.476 210 132 5 260 469 50 245 1.91e-18 85.5
Msa0474330 MtrunA17_Chr8g0385691 30.000 210 133 5 260 469 47 242 2.57e-18 85.1
Msa0474330 MtrunA17_Chr7g0254621 28.112 249 150 9 235 474 16 244 4.05e-13 70.9
Msa0474330 MtrunA17_Chr3g0144911 28.378 222 134 8 262 469 110 320 1.44e-11 66.6
Msa0474330 MtrunA17_Chr5g0399151 27.948 229 140 8 263 477 116 333 1.88e-11 66.6
Msa0474330 MtrunA17_Chr4g0035391 27.477 222 136 8 262 469 107 317 2.00e-11 67.0
Msa0474330 MtrunA17_Chr1g0155521 27.727 220 138 8 262 469 112 322 4.56e-11 65.9
Msa0474330 MtrunA17_Chr8g0372651 27.149 221 138 8 262 469 79 289 8.60e-11 64.7
Msa0474330 MtrunA17_Chr3g0144891 27.027 222 137 8 262 469 97 307 9.74e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0474330 AT4G09160.1 64.571 350 117 3 238 582 317 664 1.46e-169 497
Msa0474330 AT4G09160.2 64.571 350 117 3 238 582 292 639 1.48e-169 496
Msa0474330 AT4G09160.3 64.571 350 117 3 238 582 292 639 1.81e-169 496
Msa0474330 AT1G72160.1 54.244 483 193 10 116 582 19 489 5.59e-168 487
Msa0474330 AT1G22530.2 43.570 661 256 14 14 581 12 648 1.32e-150 448
Msa0474330 AT1G72150.1 51.606 498 220 10 90 581 91 573 2.79e-149 442
Msa0474330 AT1G22530.1 54.780 387 157 5 196 581 313 682 4.19e-144 432
Msa0474330 AT1G30690.1 41.455 550 267 16 51 585 30 539 3.40e-123 374
Msa0474330 AT1G30690.2 41.455 550 267 16 51 585 30 539 3.40e-123 374
Msa0474330 AT3G51670.1 46.174 379 193 8 213 585 34 407 6.64e-113 343
Msa0474330 AT1G01630.1 30.000 210 133 5 260 469 50 245 2.19e-19 88.6
Msa0474330 AT4G08690.2 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0474330 AT4G08690.1 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0474330 AT4G08690.3 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0474330 AT3G24840.1 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0474330 AT3G24840.6 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0474330 AT3G24840.4 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0474330 AT3G24840.2 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0474330 AT3G24840.7 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0474330 AT3G24840.3 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0474330 AT3G24840.5 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0474330 AT4G34580.2 26.606 218 143 6 262 469 88 298 6.92e-12 68.6
Msa0474330 AT4G34580.1 26.606 218 143 6 262 469 88 298 6.92e-12 68.6
Msa0474330 AT1G19650.1 28.829 222 133 7 262 469 104 314 7.37e-12 68.6
Msa0474330 AT2G21540.6 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0474330 AT2G21540.2 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0474330 AT2G21540.1 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0474330 AT2G21540.4 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0474330 AT2G21540.3 28.571 224 131 10 262 469 95 305 1.32e-11 67.8
Msa0474330 AT2G21540.5 28.571 224 131 10 262 469 95 305 1.32e-11 67.8
Msa0474330 AT4G39170.2 29.148 223 131 9 262 469 108 318 1.88e-11 67.4
Msa0474330 AT2G18180.2 26.957 230 146 9 253 469 71 291 1.94e-11 67.4
Msa0474330 AT4G39170.1 29.148 223 131 9 262 469 108 318 1.98e-11 67.4
Msa0474330 AT4G39180.3 27.273 231 145 8 251 469 87 306 3.02e-11 66.6
Msa0474330 AT4G39180.2 27.273 231 145 8 251 469 87 306 3.02e-11 66.6
Msa0474330 AT2G18180.1 26.957 230 146 9 253 469 71 291 3.04e-11 66.6
Msa0474330 AT4G39180.1 26.866 268 168 10 215 469 54 306 3.10e-11 66.6
Msa0474330 AT1G22180.2 25.896 251 161 6 235 478 18 250 3.27e-11 65.1
Msa0474330 AT1G22180.4 25.896 251 161 6 235 478 18 250 3.27e-11 65.1
Msa0474330 AT1G75170.2 24.889 225 128 6 259 469 43 240 5.19e-11 64.3
Msa0474330 AT1G75170.1 24.889 225 128 6 259 469 43 240 5.19e-11 64.3
Msa0474330 AT1G19650.3 28.829 222 133 7 262 469 104 314 7.06e-11 65.1

Find 174 sgRNAs with CRISPR-Local

Find 305 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATTTCTTTGTAAATTT+TGG 0.098338 3_3:+77173478 Msa0474330:three_prime_UTR
TCTCCTTCATTTAACAATTT+TGG 0.098979 3_3:+77170046 Msa0474330:five_prime_UTR
CTCGGGCTCTGGGTTGGGCT+TGG 0.133515 3_3:-77170250 None:intergenic
TCATGGGAGCTAAGAGTAAT+TGG 0.180786 3_3:+77173092 Msa0474330:CDS
CCTGTTTGTTATAACATTTA+TGG 0.191442 3_3:+77171089 Msa0474330:CDS
ACCTGTTTGGCTACAAATTC+AGG 0.192401 3_3:-77171347 None:intergenic
TCAAATAACACCGTTATTTC+CGG 0.205164 3_3:+77169879 Msa0474330:five_prime_UTR
TGTGATTCTGCTTCTGCTTC+TGG 0.205662 3_3:-77170519 None:intergenic
CAAAATTGAAGAGTTATTAT+TGG 0.213965 3_3:+77170998 Msa0474330:CDS
TTGAATTCTTATATTGTTAT+TGG 0.229427 3_3:+77173442 Msa0474330:three_prime_UTR
AATAACGATGAAAAGTATTT+TGG 0.229828 3_3:-77170074 None:intergenic
CTAAGCAAAGATGGTGAATT+TGG 0.229951 3_3:+77172365 Msa0474330:CDS
TCTTGAGTTGTGGCTATCTT+TGG 0.230004 3_3:-77170141 None:intergenic
CTTTGCAACAACTACAAAAT+TGG 0.236225 3_3:+77173218 Msa0474330:CDS
TCGGGCTCTGGGTTGGGCTT+GGG 0.258053 3_3:-77170249 None:intergenic
AAAAGCAAGTTTGTATTTGC+TGG 0.284258 3_3:+77171687 Msa0474330:CDS
ACCAAATTCACCTCAGTAAC+TGG 0.284996 3_3:-77172449 None:intergenic
TTCTGCTTCTGGTGTGGGCT+CGG 0.286758 3_3:-77170508 None:intergenic
GTTCTTGAAATGGAGAATTC+TGG 0.287184 3_3:+77171172 Msa0474330:CDS
GTTCTGTTCATCTTTCTTGT+TGG 0.294746 3_3:-77170559 None:intergenic
ATGATAGAGGGGTTGTTTCG+TGG 0.300356 3_3:-77169994 None:intergenic
AGTGCTTTCTTCTCAGCTTC+AGG 0.302927 3_3:-77170390 None:intergenic
CGGGCCATGGACCCACTTAA+TGG 0.315039 3_3:+77169899 Msa0474330:five_prime_UTR
CCCCATATTGAAACCTCTTC+TGG 0.316394 3_3:-77170852 None:intergenic
TGGTCTTGGAGAAGAGTATT+AGG 0.324429 3_3:+77171192 Msa0474330:CDS
AGAGAGAAAAGTTCTTGAAA+TGG 0.327651 3_3:+77171162 Msa0474330:CDS
ACAAGCATATAGATCAAATT+AGG 0.328233 3_3:-77173506 None:intergenic
TCCACCACCTTTGGTTCCTC+TGG 0.337500 3_3:-77170798 None:intergenic
CCATAAATGTTATAACAAAC+AGG 0.338368 3_3:-77171089 None:intergenic
TAGAAGAGTCTCAGCTGATT+TGG 0.338628 3_3:-77171715 None:intergenic
CGTTGGCTTCAAATTCTAAC+AGG 0.344786 3_3:+77169810 Msa0474330:five_prime_UTR
GTCTGTGCCAGATTGGAATC+TGG 0.348569 3_3:-77169932 None:intergenic
TTCTTTCACTTTGAAGTCTC+TGG 0.352608 3_3:-77170931 None:intergenic
TTCTTGTTGGTCTCTTCAGC+AGG 0.368545 3_3:-77170546 None:intergenic
AACAGGCTGGGCTATGTAAC+TGG 0.372569 3_3:+77169827 Msa0474330:five_prime_UTR
GAGCAACTTTCAGTCAAATA+TGG 0.373495 3_3:+77172338 Msa0474330:CDS
GGAGAAGAGTATTAGGAAGT+TGG 0.378620 3_3:+77171199 Msa0474330:CDS
GTCTTAATCAAAATCAACTA+TGG 0.378649 3_3:+77170107 Msa0474330:exon
GAAGAAGCTTCCACCACCTT+TGG 0.382704 3_3:-77170807 None:intergenic
AACCAGAAGAGGTTTCAATA+TGG 0.384526 3_3:+77170850 Msa0474330:CDS
AGATGGTGAATTTGGAATTA+CGG 0.390975 3_3:+77172373 Msa0474330:CDS
AAGCAATTGAAGGGCTTGTT+TGG 0.391690 3_3:-77171312 None:intergenic
TCAGCTTCAGTTGTTGTTGT+TGG 0.393012 3_3:-77170651 None:intergenic
AATCAGCTGAGACTCTTCTA+AGG 0.396379 3_3:+77171718 Msa0474330:CDS
CTTTCTTAATCAGAAGTTGA+AGG 0.397210 3_3:-77173320 None:intergenic
TCCTGAATTTGTAGCCAAAC+AGG 0.397889 3_3:+77171346 Msa0474330:CDS
TAGTGTGATGGGGATGATAG+AGG 0.402579 3_3:-77170007 None:intergenic
TCTGCTTCTGCTTCTGGTGT+GGG 0.403305 3_3:-77170513 None:intergenic
TCTGCTTCTGGTGTGGGCTC+GGG 0.406078 3_3:-77170507 None:intergenic
CAAATAACACCGTTATTTCC+GGG 0.410392 3_3:+77169880 Msa0474330:five_prime_UTR
AACTACAAAATTGGTGAACC+TGG 0.412559 3_3:+77173227 Msa0474330:CDS
GCTAGTAGGCCCGCCGTGTT+TGG 0.413300 3_3:+77169849 Msa0474330:five_prime_UTR
GGCTTCAAATTCTAACAGGC+TGG 0.417907 3_3:+77169814 Msa0474330:five_prime_UTR
CTTAGAGCTTGAATTGTCAA+TGG 0.418583 3_3:-77173264 None:intergenic
GTAAAGTATTAGTACCTGTT+TGG 0.422219 3_3:-77171360 None:intergenic
TGTTAAATGAAGGAGATTGA+TGG 0.423877 3_3:-77170039 None:intergenic
TTTGTTCTTTGTGTAAGAAA+AGG 0.424626 3_3:-77171666 None:intergenic
ACTTGCTCCTCCTCAGCAAC+AGG 0.426842 3_3:-77170171 None:intergenic
TTAATCAGAAGTTGAAGGCT+TGG 0.430333 3_3:-77173315 None:intergenic
TCTGTGTCCCAATAGTAAAA+AGG 0.437051 3_3:+77173575 Msa0474330:three_prime_UTR
GAGAAAACTGTTTACATGCA+TGG 0.439519 3_3:+77171047 Msa0474330:CDS
TCTGTGCCAGATTGGAATCT+GGG 0.439720 3_3:-77169931 None:intergenic
GATTGGAATCTGGGCTCAAC+AGG 0.442208 3_3:-77169922 None:intergenic
TCAGTTGTTGTTGTTGGTAG+TGG 0.442852 3_3:-77170645 None:intergenic
TTCTGCTTCTGCTTCTGGTG+TGG 0.443749 3_3:-77170514 None:intergenic
ACTGGGCTTGGAGTAGTTGA+AGG 0.447024 3_3:-77170471 None:intergenic
TTCTTTGTCAGCTGGAACAG+TGG 0.452483 3_3:-77170217 None:intergenic
TCCAGAGTCTGGTTCGTTCA+AGG 0.461611 3_3:+77170341 Msa0474330:CDS
AGCTTGTCTAAGCTCCCATT+TGG 0.463609 3_3:-77171288 None:intergenic
ACCAGAAGAGGTTTCAATAT+GGG 0.467828 3_3:+77170851 Msa0474330:CDS
CATGGGAGCTAAGAGTAATT+GGG 0.468056 3_3:+77173093 Msa0474330:CDS
AATTATCTTGAAGCAATTGA+AGG 0.470059 3_3:-77171322 None:intergenic
GAAATGGAGAATTCTGGTCT+TGG 0.470951 3_3:+77171178 Msa0474330:CDS
ATTCTCCTGGACCTACCAAA+TGG 0.471318 3_3:+77171273 Msa0474330:CDS
AATGATCTTAAGAATTCTCC+TGG 0.479288 3_3:+77171260 Msa0474330:CDS
AGGACCAGTCTGTGCCAGAT+TGG 0.480098 3_3:-77169939 None:intergenic
GTGTAGCTTCCTTCTGAACT+TGG 0.481631 3_3:-77173146 None:intergenic
AGCTCCCATTTGGTAGGTCC+AGG 0.482236 3_3:-77171278 None:intergenic
TTGTAGTTGTTGCAAAGCAC+TGG 0.484047 3_3:-77173212 None:intergenic
TGTTAATCATTATCTTGTGT+TGG 0.484728 3_3:+77173349 Msa0474330:three_prime_UTR
GGCAGTGCCTGTTGCTGAGG+AGG 0.486650 3_3:+77170164 Msa0474330:CDS
AGGACTTATCATTCTGTTCA+CGG 0.488350 3_3:-77171646 None:intergenic
TCATCAGATTCTTTGTCAGC+TGG 0.490379 3_3:-77170225 None:intergenic
TGATAGAGGGGTTGTTTCGT+GGG 0.492244 3_3:-77169993 None:intergenic
TTATTATTGGATGAAAACTT+AGG 0.493600 3_3:+77171011 Msa0474330:CDS
AAGGAAGAGAGCACTATTGT+TGG 0.493837 3_3:+77170360 Msa0474330:CDS
TCACCAAAATTGTTAAATGA+AGG 0.494140 3_3:-77170049 None:intergenic
TGCAGAACTGTCTACTCTCA+TGG 0.494143 3_3:+77173075 Msa0474330:intron
GGGCTATGTAACTGGCTAGT+AGG 0.494657 3_3:+77169835 Msa0474330:five_prime_UTR
ACACCGTTATTTCCGGGCCA+TGG 0.497800 3_3:+77169886 Msa0474330:five_prime_UTR
AGATGAACAGAACAATGAAC+AGG 0.499148 3_3:+77170569 Msa0474330:CDS
TGTCTAAGCTCCCATTTGGT+AGG 0.502674 3_3:-77171284 None:intergenic
GGAGAGTGGTGTAGTGTGAT+GGG 0.505695 3_3:-77170018 None:intergenic
TTCTCCTGGACCTACCAAAT+GGG 0.508042 3_3:+77171274 Msa0474330:CDS
CATTAAGTGGGTCCATGGCC+CGG 0.511063 3_3:-77169898 None:intergenic
TATTTGACTGAAAGTTGCTC+AGG 0.515624 3_3:-77172335 None:intergenic
GGTCCATGGCCCGGAAATAA+CGG 0.519376 3_3:-77169889 None:intergenic
ATCAAAATCAACTATGGCTG+AGG 0.520145 3_3:+77170113 Msa0474330:exon
TATGGTGGACTAAGCAAAGA+TGG 0.520271 3_3:+77172356 Msa0474330:CDS
GCTTCTTCTGTAGATGAAGA+TGG 0.521848 3_3:+77170720 Msa0474330:CDS
GTTGTTGTTGTTGGTAGTGG+TGG 0.522925 3_3:-77170642 None:intergenic
AAAGAGTGGGGCGGATGAGA+AGG 0.524597 3_3:-77169959 None:intergenic
CTGTTAGAATTTGAAGCCAA+CGG 0.527127 3_3:-77169809 None:intergenic
TCCTTGAACGAACCAGACTC+TGG 0.528848 3_3:-77170342 None:intergenic
ATGGTGAGAACCACTTTCCC+AGG 0.529543 3_3:-77173245 None:intergenic
GTTGAGCCCAGATTCCAATC+TGG 0.532116 3_3:+77169925 Msa0474330:five_prime_UTR
TTGTTGGTCTCTTCAGCAGG+TGG 0.536894 3_3:-77170543 None:intergenic
GGGAAGCAGTTATAAAGAGT+GGG 0.537800 3_3:-77169972 None:intergenic
AATTGGTGAACCTGGGAAAG+TGG 0.538369 3_3:+77173235 Msa0474330:CDS
TCCAGAGGAACCAAAGGTGG+TGG 0.538723 3_3:+77170797 Msa0474330:CDS
AGTGTGATGGGGATGATAGA+GGG 0.542639 3_3:-77170006 None:intergenic
CCAGAAGAGGTTTCAATATG+GGG 0.547282 3_3:+77170852 Msa0474330:CDS
GATTCCAATCTGGCACAGAC+TGG 0.548284 3_3:+77169935 Msa0474330:five_prime_UTR
CAGGCCATTAAGTGGGTCCA+TGG 0.548475 3_3:-77169903 None:intergenic
TGGAGAGTGGTGTAGTGTGA+TGG 0.549657 3_3:-77170019 None:intergenic
GAGTTTGTACCAAGTTCAGA+AGG 0.553555 3_3:+77173137 Msa0474330:CDS
GCTTCAAATTCTAACAGGCT+GGG 0.554058 3_3:+77169815 Msa0474330:five_prime_UTR
ATTATCTTGAAGCAATTGAA+GGG 0.556517 3_3:-77171321 None:intergenic
GGGTTGGAAAGAAATGTGAA+TGG 0.560559 3_3:+77169784 None:intergenic
AGAAATGTGAATGGAACCGT+TGG 0.566113 3_3:+77169793 Msa0474330:five_prime_UTR
ACAACAACAACAACCAGAAG+AGG 0.568417 3_3:+77170839 Msa0474330:CDS
GACTCTCTACCAAACACGGC+GGG 0.569158 3_3:-77169858 None:intergenic
GGCTCAACAGGCCATTAAGT+GGG 0.569499 3_3:-77169910 None:intergenic
GGGCTCAACAGGCCATTAAG+TGG 0.571417 3_3:-77169911 None:intergenic
TGAAGGAGATTGATGGAGAG+TGG 0.575247 3_3:-77170032 None:intergenic
TTATGGAGAGTTTGATAACA+AGG 0.578141 3_3:+77171106 Msa0474330:CDS
ATGCATGGATATGACAAAGA+AGG 0.578689 3_3:+77171062 Msa0474330:CDS
AGAGGCAGTGCCTGTTGCTG+AGG 0.578794 3_3:+77170161 Msa0474330:CDS
GTGTGATGGGGATGATAGAG+GGG 0.579996 3_3:-77170005 None:intergenic
CTCAGTAACTGGAAATTCCA+CGG 0.580825 3_3:-77172438 None:intergenic
GGGGAAGCAGTTATAAAGAG+TGG 0.582308 3_3:-77169973 None:intergenic
GATAGAGGGGTTGTTTCGTG+GGG 0.583769 3_3:-77169992 None:intergenic
GTTCTATCGTCTGCAAGAAG+TGG 0.593136 3_3:-77170879 None:intergenic
AAAGATAGCCACAACTCAAG+AGG 0.593760 3_3:+77170143 Msa0474330:CDS
GGTGGTGGAAGCTTCTTCAC+CGG 0.595988 3_3:+77170812 Msa0474330:CDS
ACTGGGCTTTGATACCTCCT+CGG 0.596592 3_3:-77170304 None:intergenic
AGGCACTGCCTCTTGAGTTG+TGG 0.597680 3_3:-77170151 None:intergenic
ACTACAAAATTGGTGAACCT+GGG 0.600044 3_3:+77173228 Msa0474330:CDS
TGATCAAGAACACTATCCGT+TGG 0.601471 3_3:+77170964 Msa0474330:CDS
GCAGAACTGTCTACTCTCAT+GGG 0.603658 3_3:+77173076 Msa0474330:intron
TGTTGCTGAGGAGGAGCAAG+TGG 0.604048 3_3:+77170173 Msa0474330:CDS
ATTGGGTGGGACATAAGCTA+TGG 0.608625 3_3:+77173110 Msa0474330:CDS
AGCAGAAAAGAAAGAAACAG+AGG 0.609236 3_3:+77170692 Msa0474330:CDS
CAACTTTCAGTCAAATATGG+TGG 0.612687 3_3:+77172341 Msa0474330:CDS
ATCAGTTCCAGAGGAACCAA+AGG 0.614270 3_3:+77170791 Msa0474330:CDS
ACAATAGTGCAAATACCACC+AGG 0.619339 3_3:-77171230 None:intergenic
AAACAACACTGCTGAGACCG+AGG 0.619853 3_3:+77170287 Msa0474330:CDS
GGGAGCTAAGAGTAATTGGG+TGG 0.623613 3_3:+77173096 Msa0474330:CDS
ATTTGACTCTCTACCAAACA+CGG 0.624946 3_3:-77169862 None:intergenic
AGTTCCAGAGGAACCAAAGG+TGG 0.628144 3_3:+77170794 Msa0474330:CDS
CATAAATGTTATAACAAACA+GGG 0.628980 3_3:-77171088 None:intergenic
AGAGTCTCAGCTGATTTGGA+AGG 0.633487 3_3:-77171711 None:intergenic
GAGAGTGGTGTAGTGTGATG+GGG 0.637501 3_3:-77170017 None:intergenic
TTGAAGGCTTGGTCTTCAAG+CGG 0.637531 3_3:-77173304 None:intergenic
AGGTGTTTATCAATGTGCCA+TGG 0.641137 3_3:+77171613 Msa0474330:intron
TGGAGAGTTTGATAACAAGG+AGG 0.651054 3_3:+77171109 Msa0474330:CDS
TGTAGCTTCATCAGTTCCAG+AGG 0.651074 3_3:+77170782 Msa0474330:CDS
CAGAAGAGGTTTCAATATGG+GGG 0.653215 3_3:+77170853 Msa0474330:CDS
CGTGGAATTTCCAGTTACTG+AGG 0.660408 3_3:+77172439 Msa0474330:CDS
AGAAATTACAGTGAGGCCTG+CGG 0.663813 3_3:+77172406 Msa0474330:CDS
GGAGCTAAGAGTAATTGGGT+GGG 0.668364 3_3:+77173097 Msa0474330:CDS
TGCTGAGGAGGAGCAAGTGG+TGG 0.671033 3_3:+77170176 Msa0474330:CDS
AGCAGTTATAAAGAGTGGGG+CGG 0.687982 3_3:-77169968 None:intergenic
AATAGTGCAAATACCACCAG+GGG 0.702016 3_3:-77171228 None:intergenic
CAATAGTGCAAATACCACCA+GGG 0.705753 3_3:-77171229 None:intergenic
TGTTTATCAATGTGCCATGG+TGG 0.706720 3_3:+77171616 Msa0474330:CDS
GGAAGCAGTTATAAAGAGTG+GGG 0.706820 3_3:-77169971 None:intergenic
GGGCTTGGAGTAGTTGAAGG+TGG 0.709064 3_3:-77170468 None:intergenic
TTCACGGCTAAGTACCACCA+TGG 0.713152 3_3:-77171630 None:intergenic
GCCTGCGGCAAAACACACCG+TGG 0.718457 3_3:+77172421 Msa0474330:CDS
TGACTCTCTACCAAACACGG+CGG 0.728466 3_3:-77169859 None:intergenic
CAACACTGCTGAGACCGAGG+AGG 0.737926 3_3:+77170290 Msa0474330:CDS
CTGTTACAGAAATTACAGTG+AGG 0.780489 3_3:+77172399 Msa0474330:CDS
GAGGAGGTATCAAAGCCCAG+TGG 0.800821 3_3:+77170306 Msa0474330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAGTATTATCTATTT+CGG - chr3_3:77173005-77173024 None:intergenic 10.0%
!!! ATAAATTTCTTTGTAAATTT+TGG + chr3_3:77173478-77173497 Msa0474330:three_prime_UTR 10.0%
!!! TTTATTTGATTTTGTTTTGT+AGG + chr3_3:77171593-77171612 Msa0474330:intron 15.0%
!!! TTTATTACTTTTTTAAACGT+GGG - chr3_3:77171954-77171973 None:intergenic 15.0%
!!! TTTTATTACTTTTTTAAACG+TGG - chr3_3:77171955-77171974 None:intergenic 15.0%
!!! CATATTTAAGTATTTTTCAT+TGG + chr3_3:77172133-77172152 Msa0474330:intron 15.0%
!!! TTTAATAAAAGTACCTATTA+AGG - chr3_3:77172196-77172215 None:intergenic 15.0%
!!! ATGTTTCTTTGATATTTTAA+AGG + chr3_3:77172969-77172988 Msa0474330:intron 15.0%
!!! TGTTTCTTTGATATTTTAAA+GGG + chr3_3:77172970-77172989 Msa0474330:intron 15.0%
!! TTGAATTCTTATATTGTTAT+TGG + chr3_3:77173442-77173461 Msa0474330:three_prime_UTR 15.0%
!!! AATAACGATGAAAAGTATTT+TGG - chr3_3:77170077-77170096 None:intergenic 20.0%
!! CAAAATTGAAGAGTTATTAT+TGG + chr3_3:77170998-77171017 Msa0474330:CDS 20.0%
!! TTATTATTGGATGAAAACTT+AGG + chr3_3:77171011-77171030 Msa0474330:CDS 20.0%
!! CATAAATGTTATAACAAACA+GGG - chr3_3:77171091-77171110 None:intergenic 20.0%
!! AAATTTAAGAACAACAATTG+TGG - chr3_3:77171503-77171522 None:intergenic 20.0%
!! TATTACTTCTTTATGTAAGT+GGG - chr3_3:77171993-77172012 None:intergenic 20.0%
!! TTATTACTTCTTTATGTAAG+TGG - chr3_3:77171994-77172013 None:intergenic 20.0%
!!! TATTACTCATTTTTACAAGT+GGG - chr3_3:77172075-77172094 None:intergenic 20.0%
!! TGTTTAATATGTAAAGATAG+TGG + chr3_3:77172108-77172127 Msa0474330:intron 20.0%
!! GTTTAATATGTAAAGATAGT+GGG + chr3_3:77172109-77172128 Msa0474330:intron 20.0%
!!! TAGTGTCTTAATTTGTTTTA+TGG + chr3_3:77172293-77172312 Msa0474330:intron 20.0%
!! ACAAATTGTTGCATAAAATA+AGG - chr3_3:77172627-77172646 None:intergenic 20.0%
!! AAGGAATCATAAAATAAACA+TGG + chr3_3:77172690-77172709 Msa0474330:intron 20.0%
!!! ATGACACATTAATTTTTTGA+GGG + chr3_3:77172821-77172840 Msa0474330:intron 20.0%
!!! TTCTTTTTGCTGTATTATTT+TGG + chr3_3:77173390-77173409 Msa0474330:three_prime_UTR 20.0%
!!! TATTTTGGTTATTACTATTG+AGG + chr3_3:77173405-77173424 Msa0474330:three_prime_UTR 20.0%
!!! ATTTTGGTTATTACTATTGA+GGG + chr3_3:77173406-77173425 Msa0474330:three_prime_UTR 20.0%
!!! AATTTCTTTGTAAATTTTGG+TGG + chr3_3:77173481-77173500 Msa0474330:three_prime_UTR 20.0%
!!! ATTTCTTTGTAAATTTTGGT+GGG + chr3_3:77173482-77173501 Msa0474330:three_prime_UTR 20.0%
!! TCTCCTTCATTTAACAATTT+TGG + chr3_3:77170046-77170065 Msa0474330:five_prime_UTR 25.0%
! TCACCAAAATTGTTAAATGA+AGG - chr3_3:77170052-77170071 None:intergenic 25.0%
! GTCTTAATCAAAATCAACTA+TGG + chr3_3:77170107-77170126 Msa0474330:exon 25.0%
! CCATAAATGTTATAACAAAC+AGG - chr3_3:77171092-77171111 None:intergenic 25.0%
!!! CCTGTTTGTTATAACATTTA+TGG + chr3_3:77171089-77171108 Msa0474330:CDS 25.0%
! ATTATCTTGAAGCAATTGAA+GGG - chr3_3:77171324-77171343 None:intergenic 25.0%
! AATTATCTTGAAGCAATTGA+AGG - chr3_3:77171325-77171344 None:intergenic 25.0%
! GCAATTAATATAACTGAAAC+TGG - chr3_3:77171403-77171422 None:intergenic 25.0%
! TTTGTTCTTTGTGTAAGAAA+AGG - chr3_3:77171669-77171688 None:intergenic 25.0%
! GTAATAAAATGTGTACTACT+TGG + chr3_3:77171966-77171985 Msa0474330:intron 25.0%
! GTAATAAAATGTGTACTACT+TGG + chr3_3:77171966-77171985 Msa0474330:intron 25.0%
! TATTACTTCTTTATGCAAGT+GGG - chr3_3:77172034-77172053 None:intergenic 25.0%
! TTATTACTTCTTTATGCAAG+TGG - chr3_3:77172035-77172054 None:intergenic 25.0%
! GTAATAAAATGTGTACTACT+TGG + chr3_3:77171966-77171985 Msa0474330:intron 25.0%
!!! CTATTACTCATTTTTACAAG+TGG - chr3_3:77172076-77172095 None:intergenic 25.0%
!!! TTTTTTAGATTGAGTATCCA+AGG + chr3_3:77172515-77172534 Msa0474330:intron 25.0%
!! TTTTTAGATTGAGTATCCAA+GGG + chr3_3:77172516-77172535 Msa0474330:intron 25.0%
! AATATAAATATGAAGACCAG+AGG - chr3_3:77172578-77172597 None:intergenic 25.0%
! TAAACAAACAAAAGAGGAAA+AGG - chr3_3:77172604-77172623 None:intergenic 25.0%
! ATAAGGTAAACAAACAAAAG+AGG - chr3_3:77172610-77172629 None:intergenic 25.0%
! CTCAAAAAATTAATGTGTCA+TGG - chr3_3:77172822-77172841 None:intergenic 25.0%
!!! CATGACACATTAATTTTTTG+AGG + chr3_3:77172820-77172839 Msa0474330:intron 25.0%
!!! TTAATTTTTTGAGGGTTCAA+AGG + chr3_3:77172829-77172848 Msa0474330:intron 25.0%
!!! ATGCATTGAAATTTTGTTGT+TGG + chr3_3:77173044-77173063 Msa0474330:intron 25.0%
!!! TGCATTGAAATTTTGTTGTT+GGG + chr3_3:77173045-77173064 Msa0474330:intron 25.0%
! TGTTAATCATTATCTTGTGT+TGG + chr3_3:77173349-77173368 Msa0474330:three_prime_UTR 25.0%
!!! TTTTGGTTATTACTATTGAG+GGG + chr3_3:77173407-77173426 Msa0474330:three_prime_UTR 25.0%
!!! TTGTTATTGGTCTGTTTTAT+TGG + chr3_3:77173455-77173474 Msa0474330:three_prime_UTR 25.0%
!!! TTTCTTTGTAAATTTTGGTG+GGG + chr3_3:77173483-77173502 Msa0474330:three_prime_UTR 25.0%
! ACAAGCATATAGATCAAATT+AGG - chr3_3:77173509-77173528 None:intergenic 25.0%
!! CGTTTTTCCTTTTTACTATT+GGG - chr3_3:77173585-77173604 None:intergenic 25.0%
!!! TCGTTTTTCCTTTTTACTAT+TGG - chr3_3:77173586-77173605 None:intergenic 25.0%
! TCAAATAACACCGTTATTTC+CGG + chr3_3:77169879-77169898 Msa0474330:five_prime_UTR 30.0%
TGTTAAATGAAGGAGATTGA+TGG - chr3_3:77170042-77170061 None:intergenic 30.0%
! TTCTTTTCTGCTTCTTCTTT+TGG - chr3_3:77170684-77170703 None:intergenic 30.0%
! TTTTGAACTCTTTTCTCCAA+CGG - chr3_3:77170983-77171002 None:intergenic 30.0%
! TTATGGAGAGTTTGATAACA+AGG + chr3_3:77171106-77171125 Msa0474330:CDS 30.0%
AGAGAGAAAAGTTCTTGAAA+TGG + chr3_3:77171162-77171181 Msa0474330:CDS 30.0%
AATGATCTTAAGAATTCTCC+TGG + chr3_3:77171260-77171279 Msa0474330:CDS 30.0%
! GTAAAGTATTAGTACCTGTT+TGG - chr3_3:77171363-77171382 None:intergenic 30.0%
AAACATGCACTAAATAGATG+CGG + chr3_3:77171477-77171496 Msa0474330:intron 30.0%
AAAAGCAAGTTTGTATTTGC+TGG + chr3_3:77171687-77171706 Msa0474330:CDS 30.0%
CTAATGGCAATACACTAATA+AGG - chr3_3:77172162-77172181 None:intergenic 30.0%
! AGATGGTGAATTTGGAATTA+CGG + chr3_3:77172373-77172392 Msa0474330:CDS 30.0%
AGATACTAGATGCTTTATCA+AGG + chr3_3:77172671-77172690 Msa0474330:intron 30.0%
!! ATGGTCATTTTGTTAAGTCA+TGG - chr3_3:77172803-77172822 None:intergenic 30.0%
! GTCTATGATTTTGCTAAACT+TGG - chr3_3:77172898-77172917 None:intergenic 30.0%
!! AAGCAATTTTTCTAGTCTTC+TGG - chr3_3:77173181-77173200 None:intergenic 30.0%
CTTTGCAACAACTACAAAAT+TGG + chr3_3:77173218-77173237 Msa0474330:CDS 30.0%
CTTTCTTAATCAGAAGTTGA+AGG - chr3_3:77173323-77173342 None:intergenic 30.0%
! CTGTTAGAATTTGAAGCCAA+CGG - chr3_3:77169812-77169831 None:intergenic 35.0%
ATTTGACTCTCTACCAAACA+CGG - chr3_3:77169865-77169884 None:intergenic 35.0%
! CAAATAACACCGTTATTTCC+GGG + chr3_3:77169880-77169899 Msa0474330:five_prime_UTR 35.0%
ATCAAAATCAACTATGGCTG+AGG + chr3_3:77170113-77170132 Msa0474330:exon 35.0%
!!! GGAATTTTTTCATCACCACT+GGG - chr3_3:77170324-77170343 None:intergenic 35.0%
!!! TGGAATTTTTTCATCACCAC+TGG - chr3_3:77170325-77170344 None:intergenic 35.0%
GATGAAAAAATTCCAGAGTC+TGG + chr3_3:77170330-77170349 Msa0474330:CDS 35.0%
! TTTTGTTCTTTAACTGGGCT+TGG - chr3_3:77170486-77170505 None:intergenic 35.0%
GTTCTGTTCATCTTTCTTGT+TGG - chr3_3:77170562-77170581 None:intergenic 35.0%
AGATGAACAGAACAATGAAC+AGG + chr3_3:77170569-77170588 Msa0474330:CDS 35.0%
AGCAGAAAAGAAAGAAACAG+AGG + chr3_3:77170692-77170711 Msa0474330:CDS 35.0%
AACCAGAAGAGGTTTCAATA+TGG + chr3_3:77170850-77170869 Msa0474330:CDS 35.0%
ACCAGAAGAGGTTTCAATAT+GGG + chr3_3:77170851-77170870 Msa0474330:CDS 35.0%
!! TTCTTTCACTTTGAAGTCTC+TGG - chr3_3:77170934-77170953 None:intergenic 35.0%
GAGAAAACTGTTTACATGCA+TGG + chr3_3:77171047-77171066 Msa0474330:CDS 35.0%
ATGCATGGATATGACAAAGA+AGG + chr3_3:77171062-77171081 Msa0474330:CDS 35.0%
GTTCTTGAAATGGAGAATTC+TGG + chr3_3:77171172-77171191 Msa0474330:CDS 35.0%
TTTAGTGCATGTTTGGTATC+AGG - chr3_3:77171471-77171490 None:intergenic 35.0%
GCATCTATTTAGTGCATGTT+TGG - chr3_3:77171478-77171497 None:intergenic 35.0%
AGGACTTATCATTCTGTTCA+CGG - chr3_3:77171649-77171668 None:intergenic 35.0%
! TTTGTTAGAGCATCTCCAAT+GGG + chr3_3:77171804-77171823 Msa0474330:intron 35.0%
! ATTTTTCTTCCTCCAATGGA+GGG - chr3_3:77171867-77171886 None:intergenic 35.0%
! AATTTTTCTTCCTCCAATGG+AGG - chr3_3:77171868-77171887 None:intergenic 35.0%
! CTCAATTTTTCTTCCTCCAA+TGG - chr3_3:77171871-77171890 None:intergenic 35.0%
TTAGTTATCTAGGAGAGAGA+GGG - chr3_3:77171926-77171945 None:intergenic 35.0%
TCTCTCTCCTAGATAACTAA+CGG + chr3_3:77171926-77171945 Msa0474330:intron 35.0%
TAAGGACTCAATGACTCTAA+TGG - chr3_3:77172178-77172197 None:intergenic 35.0%
AGAGTCATTGAGTCCTTAAT+AGG + chr3_3:77172180-77172199 Msa0474330:intron 35.0%
TTAATAAGTGATGTGTCACG+TGG + chr3_3:77172222-77172241 Msa0474330:intron 35.0%
TAATAAGTGATGTGTCACGT+GGG + chr3_3:77172223-77172242 Msa0474330:intron 35.0%
CATTTAAGGAGTCCTTGTTA+GGG + chr3_3:77172249-77172268 Msa0474330:intron 35.0%
TATTTGACTGAAAGTTGCTC+AGG - chr3_3:77172338-77172357 None:intergenic 35.0%
GAGCAACTTTCAGTCAAATA+TGG + chr3_3:77172338-77172357 Msa0474330:CDS 35.0%
CAACTTTCAGTCAAATATGG+TGG + chr3_3:77172341-77172360 Msa0474330:CDS 35.0%
CTAAGCAAAGATGGTGAATT+TGG + chr3_3:77172365-77172384 Msa0474330:CDS 35.0%
CTGTTACAGAAATTACAGTG+AGG + chr3_3:77172399-77172418 Msa0474330:CDS 35.0%
! TTACCTTTCTGTGTTTTGTC+AGG + chr3_3:77172483-77172502 Msa0474330:intron 35.0%
AATCCTGACAAAACACAGAA+AGG - chr3_3:77172489-77172508 None:intergenic 35.0%
! TGTGTTTTGTCAGGATTGAT+TGG + chr3_3:77172492-77172511 Msa0474330:intron 35.0%
TGGATTGTGTAATCTTTCCT+AGG + chr3_3:77172549-77172568 Msa0474330:intron 35.0%
ATCTAGTATCTCTCAAGTCA+GGG - chr3_3:77172662-77172681 None:intergenic 35.0%
GGAAACAGACATACTTCAAT+TGG + chr3_3:77172711-77172730 Msa0474330:intron 35.0%
! TGGCATTGTCATTGTGATTT+TGG - chr3_3:77172865-77172884 None:intergenic 35.0%
AACTACAAAATTGGTGAACC+TGG + chr3_3:77173227-77173246 Msa0474330:CDS 35.0%
ACTACAAAATTGGTGAACCT+GGG + chr3_3:77173228-77173247 Msa0474330:CDS 35.0%
CTTAGAGCTTGAATTGTCAA+TGG - chr3_3:77173267-77173286 None:intergenic 35.0%
TTAATCAGAAGTTGAAGGCT+TGG - chr3_3:77173318-77173337 None:intergenic 35.0%
TCTGTGTCCCAATAGTAAAA+AGG + chr3_3:77173575-77173594 Msa0474330:three_prime_UTR 35.0%
AGAAATGTGAATGGAACCGT+TGG + chr3_3:77169793-77169812 Msa0474330:five_prime_UTR 40.0%
!! CGTTGGCTTCAAATTCTAAC+AGG + chr3_3:77169810-77169829 Msa0474330:five_prime_UTR 40.0%
GCTTCAAATTCTAACAGGCT+GGG + chr3_3:77169815-77169834 Msa0474330:five_prime_UTR 40.0%
GGAAGCAGTTATAAAGAGTG+GGG - chr3_3:77169974-77169993 None:intergenic 40.0%
GGGAAGCAGTTATAAAGAGT+GGG - chr3_3:77169975-77169994 None:intergenic 40.0%
! TCTTGAGTTGTGGCTATCTT+TGG - chr3_3:77170144-77170163 None:intergenic 40.0%
AAAGATAGCCACAACTCAAG+AGG + chr3_3:77170143-77170162 Msa0474330:CDS 40.0%
TCATCAGATTCTTTGTCAGC+TGG - chr3_3:77170228-77170247 None:intergenic 40.0%
!! TTTTCAACGGGCTTTTTCTC+GGG - chr3_3:77170270-77170289 None:intergenic 40.0%
!! GTTTTCAACGGGCTTTTTCT+CGG - chr3_3:77170271-77170290 None:intergenic 40.0%
!!! TCAGCAGTGTTGTTTTCAAC+GGG - chr3_3:77170282-77170301 None:intergenic 40.0%
!!! CTCAGCAGTGTTGTTTTCAA+CGG - chr3_3:77170283-77170302 None:intergenic 40.0%
!! AAGGAAGAGAGCACTATTGT+TGG + chr3_3:77170360-77170379 Msa0474330:CDS 40.0%
! CGGGCTTTTGTTCTTTAACT+GGG - chr3_3:77170491-77170510 None:intergenic 40.0%
! TCGGGCTTTTGTTCTTTAAC+TGG - chr3_3:77170492-77170511 None:intergenic 40.0%
! TCAGTTGTTGTTGTTGGTAG+TGG - chr3_3:77170648-77170667 None:intergenic 40.0%
! TCAGCTTCAGTTGTTGTTGT+TGG - chr3_3:77170654-77170673 None:intergenic 40.0%
GCTTCTTCTGTAGATGAAGA+TGG + chr3_3:77170720-77170739 Msa0474330:CDS 40.0%
! GGTTGTTGTTGTTGTTGTTC+CGG - chr3_3:77170834-77170853 None:intergenic 40.0%
ACAACAACAACAACCAGAAG+AGG + chr3_3:77170839-77170858 Msa0474330:CDS 40.0%
CCAGAAGAGGTTTCAATATG+GGG + chr3_3:77170852-77170871 Msa0474330:CDS 40.0%
CAGAAGAGGTTTCAATATGG+GGG + chr3_3:77170853-77170872 Msa0474330:CDS 40.0%
TGATCAAGAACACTATCCGT+TGG + chr3_3:77170964-77170983 Msa0474330:CDS 40.0%
! TGGAGAGTTTGATAACAAGG+AGG + chr3_3:77171109-77171128 Msa0474330:CDS 40.0%
GAAATGGAGAATTCTGGTCT+TGG + chr3_3:77171178-77171197 Msa0474330:CDS 40.0%
!! TGGTCTTGGAGAAGAGTATT+AGG + chr3_3:77171192-77171211 Msa0474330:CDS 40.0%
!! GGAGAAGAGTATTAGGAAGT+TGG + chr3_3:77171199-77171218 Msa0474330:CDS 40.0%
AATAGTGCAAATACCACCAG+GGG - chr3_3:77171231-77171250 None:intergenic 40.0%
CAATAGTGCAAATACCACCA+GGG - chr3_3:77171232-77171251 None:intergenic 40.0%
ACAATAGTGCAAATACCACC+AGG - chr3_3:77171233-77171252 None:intergenic 40.0%
! AAGCAATTGAAGGGCTTGTT+TGG - chr3_3:77171315-77171334 None:intergenic 40.0%
TCCTGAATTTGTAGCCAAAC+AGG + chr3_3:77171346-77171365 Msa0474330:CDS 40.0%
ACCTGTTTGGCTACAAATTC+AGG - chr3_3:77171350-77171369 None:intergenic 40.0%
AGGTGTTTATCAATGTGCCA+TGG + chr3_3:77171613-77171632 Msa0474330:intron 40.0%
TGTTTATCAATGTGCCATGG+TGG + chr3_3:77171616-77171635 Msa0474330:CDS 40.0%
TAGAAGAGTCTCAGCTGATT+TGG - chr3_3:77171718-77171737 None:intergenic 40.0%
AATCAGCTGAGACTCTTCTA+AGG + chr3_3:77171718-77171737 Msa0474330:CDS 40.0%
!! CTGAGACTCTTCTAAGGTAA+AGG + chr3_3:77171724-77171743 Msa0474330:intron 40.0%
TGGAGATGCTCTAACAAACT+AGG - chr3_3:77171802-77171821 None:intergenic 40.0%
! GTTTGTTAGAGCATCTCCAA+TGG + chr3_3:77171803-77171822 Msa0474330:intron 40.0%
GAAAAATTGAGTCCCCGTTA+AGG + chr3_3:77171880-77171899 Msa0474330:intron 40.0%
TATAGTTGCCCGTTCCTTAA+CGG - chr3_3:77171897-77171916 None:intergenic 40.0%
AAGGAACGGGCAACTATATA+AGG + chr3_3:77171899-77171918 Msa0474330:intron 40.0%
GTTAGTTATCTAGGAGAGAG+AGG - chr3_3:77171927-77171946 None:intergenic 40.0%
CTCTCTCCTAGATAACTAAC+GGG + chr3_3:77171927-77171946 Msa0474330:intron 40.0%
AATAAGTGATGTGTCACGTG+GGG + chr3_3:77172224-77172243 Msa0474330:intron 40.0%
CCTAACAAGGACTCCTTAAA+TGG - chr3_3:77172251-77172270 None:intergenic 40.0%
CCATTTAAGGAGTCCTTGTT+AGG + chr3_3:77172248-77172267 Msa0474330:intron 40.0%
ACAATGGTAGTTCCCTAACA+AGG - chr3_3:77172264-77172283 None:intergenic 40.0%
TTGTTAGGGAACTACCATTG+TGG + chr3_3:77172263-77172282 Msa0474330:intron 40.0%
!! TATGGTGGACTAAGCAAAGA+TGG + chr3_3:77172356-77172375 Msa0474330:CDS 40.0%
CTCAGTAACTGGAAATTCCA+CGG - chr3_3:77172441-77172460 None:intergenic 40.0%
TCCAGTTACTGAGGTGAATT+TGG + chr3_3:77172448-77172467 Msa0474330:intron 40.0%
ACCAAATTCACCTCAGTAAC+TGG - chr3_3:77172452-77172471 None:intergenic 40.0%
AATCTTTCCTAGGATTCCTC+TGG + chr3_3:77172559-77172578 Msa0474330:intron 40.0%
CATCTAGTATCTCTCAAGTC+AGG - chr3_3:77172663-77172682 None:intergenic 40.0%
AATGACAATGCCACGTGATT+TGG + chr3_3:77172872-77172891 Msa0474330:intron 40.0%
TCATGGGAGCTAAGAGTAAT+TGG + chr3_3:77173092-77173111 Msa0474330:CDS 40.0%
CATGGGAGCTAAGAGTAATT+GGG + chr3_3:77173093-77173112 Msa0474330:CDS 40.0%
GAGTTTGTACCAAGTTCAGA+AGG + chr3_3:77173137-77173156 Msa0474330:CDS 40.0%
TTGTAGTTGTTGCAAAGCAC+TGG - chr3_3:77173215-77173234 None:intergenic 40.0%
GGCTTCAAATTCTAACAGGC+TGG + chr3_3:77169814-77169833 Msa0474330:five_prime_UTR 45.0%
TCTGTGCCAGATTGGAATCT+GGG - chr3_3:77169934-77169953 None:intergenic 45.0%
AGCAGTTATAAAGAGTGGGG+CGG - chr3_3:77169971-77169990 None:intergenic 45.0%
GGGGAAGCAGTTATAAAGAG+TGG - chr3_3:77169976-77169995 None:intergenic 45.0%
! TGATAGAGGGGTTGTTTCGT+GGG - chr3_3:77169996-77170015 None:intergenic 45.0%
! ATGATAGAGGGGTTGTTTCG+TGG - chr3_3:77169997-77170016 None:intergenic 45.0%
! AGTGTGATGGGGATGATAGA+GGG - chr3_3:77170009-77170028 None:intergenic 45.0%
! TAGTGTGATGGGGATGATAG+AGG - chr3_3:77170010-77170029 None:intergenic 45.0%
TGAAGGAGATTGATGGAGAG+TGG - chr3_3:77170035-77170054 None:intergenic 45.0%
TTCTTTGTCAGCTGGAACAG+TGG - chr3_3:77170220-77170239 None:intergenic 45.0%
AGTGCTTTCTTCTCAGCTTC+AGG - chr3_3:77170393-77170412 None:intergenic 45.0%
TGTGATTCTGCTTCTGCTTC+TGG - chr3_3:77170522-77170541 None:intergenic 45.0%
! TTCTTGTTGGTCTCTTCAGC+AGG - chr3_3:77170549-77170568 None:intergenic 45.0%
!! GTTGTTGTTGTTGGTAGTGG+TGG - chr3_3:77170645-77170664 None:intergenic 45.0%
TGTAGCTTCATCAGTTCCAG+AGG + chr3_3:77170782-77170801 Msa0474330:CDS 45.0%
ATCAGTTCCAGAGGAACCAA+AGG + chr3_3:77170791-77170810 Msa0474330:CDS 45.0%
CCCCATATTGAAACCTCTTC+TGG - chr3_3:77170855-77170874 None:intergenic 45.0%
GTTCTATCGTCTGCAAGAAG+TGG - chr3_3:77170882-77170901 None:intergenic 45.0%
! AGGAAGTTGGATTTTACCCC+TGG + chr3_3:77171212-77171231 Msa0474330:CDS 45.0%
! AAGTTGGATTTTACCCCTGG+TGG + chr3_3:77171215-77171234 Msa0474330:CDS 45.0%
ATTCTCCTGGACCTACCAAA+TGG + chr3_3:77171273-77171292 Msa0474330:CDS 45.0%
TTCTCCTGGACCTACCAAAT+GGG + chr3_3:77171274-77171293 Msa0474330:CDS 45.0%
TGTCTAAGCTCCCATTTGGT+AGG - chr3_3:77171287-77171306 None:intergenic 45.0%
AGCTTGTCTAAGCTCCCATT+TGG - chr3_3:77171291-77171310 None:intergenic 45.0%
!! AGAGTCTCAGCTGATTTGGA+AGG - chr3_3:77171714-77171733 None:intergenic 45.0%
GTTAAGTAAGGACCTCCCAT+TGG - chr3_3:77171822-77171841 None:intergenic 45.0%
TGGGAGGTCCTTACTTAACA+AGG + chr3_3:77171823-77171842 Msa0474330:intron 45.0%
GGAGTGGTCCTTGTTAAGTA+AGG - chr3_3:77171834-77171853 None:intergenic 45.0%
ATTGAGTCCCCGTTAAGGAA+CGG + chr3_3:77171885-77171904 Msa0474330:intron 45.0%
ATAGTTGCCCGTTCCTTAAC+GGG - chr3_3:77171896-77171915 None:intergenic 45.0%
!! GACACTAAGAGCATCCACAA+TGG - chr3_3:77172280-77172299 None:intergenic 45.0%
AGAAATTACAGTGAGGCCTG+CGG + chr3_3:77172406-77172425 Msa0474330:CDS 45.0%
CGTGGAATTTCCAGTTACTG+AGG + chr3_3:77172439-77172458 Msa0474330:CDS 45.0%
TATCCAAGGGAACGTTGCAT+TGG + chr3_3:77172529-77172548 Msa0474330:intron 45.0%
AATCCAATGCAACGTTCCCT+TGG - chr3_3:77172535-77172554 None:intergenic 45.0%
ATGAAGACCAGAGGAATCCT+AGG - chr3_3:77172569-77172588 None:intergenic 45.0%
TATCTCTCAAGTCAGGGCAT+AGG - chr3_3:77172656-77172675 None:intergenic 45.0%
CTAAACTTGGCCAAATCACG+TGG - chr3_3:77172885-77172904 None:intergenic 45.0%
TGCAGAACTGTCTACTCTCA+TGG + chr3_3:77173075-77173094 Msa0474330:intron 45.0%
GCAGAACTGTCTACTCTCAT+GGG + chr3_3:77173076-77173095 Msa0474330:intron 45.0%
GGAGCTAAGAGTAATTGGGT+GGG + chr3_3:77173097-77173116 Msa0474330:CDS 45.0%
ATTGGGTGGGACATAAGCTA+TGG + chr3_3:77173110-77173129 Msa0474330:CDS 45.0%
GTGTAGCTTCCTTCTGAACT+TGG - chr3_3:77173149-77173168 None:intergenic 45.0%
AATTGGTGAACCTGGGAAAG+TGG + chr3_3:77173235-77173254 Msa0474330:CDS 45.0%
! TTGAAGGCTTGGTCTTCAAG+CGG - chr3_3:77173307-77173326 None:intergenic 45.0%
AACAGGCTGGGCTATGTAAC+TGG + chr3_3:77169827-77169846 Msa0474330:five_prime_UTR 50.0%
GGGCTATGTAACTGGCTAGT+AGG + chr3_3:77169835-77169854 Msa0474330:five_prime_UTR 50.0%
TGACTCTCTACCAAACACGG+CGG - chr3_3:77169862-77169881 None:intergenic 50.0%
GGCTCAACAGGCCATTAAGT+GGG - chr3_3:77169913-77169932 None:intergenic 50.0%
GATTGGAATCTGGGCTCAAC+AGG - chr3_3:77169925-77169944 None:intergenic 50.0%
GTTGAGCCCAGATTCCAATC+TGG + chr3_3:77169925-77169944 Msa0474330:five_prime_UTR 50.0%
GTCTGTGCCAGATTGGAATC+TGG - chr3_3:77169935-77169954 None:intergenic 50.0%
GATTCCAATCTGGCACAGAC+TGG + chr3_3:77169935-77169954 Msa0474330:five_prime_UTR 50.0%
! GATAGAGGGGTTGTTTCGTG+GGG - chr3_3:77169995-77170014 None:intergenic 50.0%
! GTGTGATGGGGATGATAGAG+GGG - chr3_3:77170008-77170027 None:intergenic 50.0%
GAGAGTGGTGTAGTGTGATG+GGG - chr3_3:77170020-77170039 None:intergenic 50.0%
GGAGAGTGGTGTAGTGTGAT+GGG - chr3_3:77170021-77170040 None:intergenic 50.0%
TGGAGAGTGGTGTAGTGTGA+TGG - chr3_3:77170022-77170041 None:intergenic 50.0%
!!! TTTTTCTCGGGCTCTGGGTT+GGG - chr3_3:77170258-77170277 None:intergenic 50.0%
AAACAACACTGCTGAGACCG+AGG + chr3_3:77170287-77170306 Msa0474330:CDS 50.0%
! ACTGGGCTTTGATACCTCCT+CGG - chr3_3:77170307-77170326 None:intergenic 50.0%
! TCCAGAGTCTGGTTCGTTCA+AGG + chr3_3:77170341-77170360 Msa0474330:CDS 50.0%
TCCTTGAACGAACCAGACTC+TGG - chr3_3:77170345-77170364 None:intergenic 50.0%
ACTGGGCTTGGAGTAGTTGA+AGG - chr3_3:77170474-77170493 None:intergenic 50.0%
TCTGCTTCTGCTTCTGGTGT+GGG - chr3_3:77170516-77170535 None:intergenic 50.0%
TTCTGCTTCTGCTTCTGGTG+TGG - chr3_3:77170517-77170536 None:intergenic 50.0%
! TTGTTGGTCTCTTCAGCAGG+TGG - chr3_3:77170546-77170565 None:intergenic 50.0%
AGTTCCAGAGGAACCAAAGG+TGG + chr3_3:77170794-77170813 Msa0474330:CDS 50.0%
GAAGAAGCTTCCACCACCTT+TGG - chr3_3:77170810-77170829 None:intergenic 50.0%
TTCACGGCTAAGTACCACCA+TGG - chr3_3:77171633-77171652 None:intergenic 50.0%
! GTTAGAGCATCTCCAATGGG+AGG + chr3_3:77171807-77171826 Msa0474330:intron 50.0%
TTGAGTCCCCGTTAAGGAAC+GGG + chr3_3:77171886-77171905 Msa0474330:intron 50.0%
TAGTTGCCCGTTCCTTAACG+GGG - chr3_3:77171895-77171914 None:intergenic 50.0%
GTGGGACCCGTTAGTTATCT+AGG - chr3_3:77171936-77171955 None:intergenic 50.0%
TGTCACGTGGGGTCCATTTA+AGG + chr3_3:77172235-77172254 Msa0474330:intron 50.0%
GGGAGCTAAGAGTAATTGGG+TGG + chr3_3:77173096-77173115 Msa0474330:CDS 50.0%
ATGGTGAGAACCACTTTCCC+AGG - chr3_3:77173248-77173267 None:intergenic 50.0%
GACTCTCTACCAAACACGGC+GGG - chr3_3:77169861-77169880 None:intergenic 55.0%
! ACACCGTTATTTCCGGGCCA+TGG + chr3_3:77169886-77169905 Msa0474330:five_prime_UTR 55.0%
GGTCCATGGCCCGGAAATAA+CGG - chr3_3:77169892-77169911 None:intergenic 55.0%
CATTAAGTGGGTCCATGGCC+CGG - chr3_3:77169901-77169920 None:intergenic 55.0%
CAGGCCATTAAGTGGGTCCA+TGG - chr3_3:77169906-77169925 None:intergenic 55.0%
GGGCTCAACAGGCCATTAAG+TGG - chr3_3:77169914-77169933 None:intergenic 55.0%
AGGACCAGTCTGTGCCAGAT+TGG - chr3_3:77169942-77169961 None:intergenic 55.0%
AAAGAGTGGGGCGGATGAGA+AGG - chr3_3:77169962-77169981 None:intergenic 55.0%
! AGGCACTGCCTCTTGAGTTG+TGG - chr3_3:77170154-77170173 None:intergenic 55.0%
ACTTGCTCCTCCTCAGCAAC+AGG - chr3_3:77170174-77170193 None:intergenic 55.0%
TGTTGCTGAGGAGGAGCAAG+TGG + chr3_3:77170173-77170192 Msa0474330:CDS 55.0%
!!! CTTTTTCTCGGGCTCTGGGT+TGG - chr3_3:77170259-77170278 None:intergenic 55.0%
!! GAGGAGGTATCAAAGCCCAG+TGG + chr3_3:77170306-77170325 Msa0474330:CDS 55.0%
GGGCTTGGAGTAGTTGAAGG+TGG - chr3_3:77170471-77170490 None:intergenic 55.0%
TTCTGCTTCTGGTGTGGGCT+CGG - chr3_3:77170511-77170530 None:intergenic 55.0%
TCCAGAGGAACCAAAGGTGG+TGG + chr3_3:77170797-77170816 Msa0474330:CDS 55.0%
! TCCACCACCTTTGGTTCCTC+TGG - chr3_3:77170801-77170820 None:intergenic 55.0%
!! GGTGGTGGAAGCTTCTTCAC+CGG + chr3_3:77170812-77170831 Msa0474330:CDS 55.0%
AGCTCCCATTTGGTAGGTCC+AGG - chr3_3:77171281-77171300 None:intergenic 55.0%
AACAAGGACCACTCCCCGTT+AGG + chr3_3:77171839-77171858 Msa0474330:intron 55.0%
ACAAGGACCACTCCCCGTTA+GGG + chr3_3:77171840-77171859 Msa0474330:intron 55.0%
TCCAATGGAGGGTCCCTAAC+GGG - chr3_3:77171856-77171875 None:intergenic 55.0%
CTCCAATGGAGGGTCCCTAA+CGG - chr3_3:77171857-77171876 None:intergenic 55.0%
CGGGCCATGGACCCACTTAA+TGG + chr3_3:77169899-77169918 Msa0474330:five_prime_UTR 60.0%
AGAGGCAGTGCCTGTTGCTG+AGG + chr3_3:77170161-77170180 Msa0474330:CDS 60.0%
TGCTGAGGAGGAGCAAGTGG+TGG + chr3_3:77170176-77170195 Msa0474330:CDS 60.0%
!! CGGGCTTTTTCTCGGGCTCT+GGG - chr3_3:77170263-77170282 None:intergenic 60.0%
!! ACGGGCTTTTTCTCGGGCTC+TGG - chr3_3:77170264-77170283 None:intergenic 60.0%
CAACACTGCTGAGACCGAGG+AGG + chr3_3:77170290-77170309 Msa0474330:CDS 60.0%
TCTGCTTCTGGTGTGGGCTC+GGG - chr3_3:77170510-77170529 None:intergenic 60.0%
CCAATGGAGGGTCCCTAACG+GGG - chr3_3:77171855-77171874 None:intergenic 60.0%
CGTTAGGGACCCTCCATTGG+AGG + chr3_3:77171855-77171874 Msa0474330:intron 60.0%
! TCCACGGTGTGTTTTGCCGC+AGG - chr3_3:77172425-77172444 None:intergenic 60.0%
GCTAGTAGGCCCGCCGTGTT+TGG + chr3_3:77169849-77169868 Msa0474330:five_prime_UTR 65.0%
GGCAGTGCCTGTTGCTGAGG+AGG + chr3_3:77170164-77170183 Msa0474330:CDS 65.0%
!! TCGGGCTCTGGGTTGGGCTT+GGG - chr3_3:77170252-77170271 None:intergenic 65.0%
CCCCGTTAGGGACCCTCCAT+TGG + chr3_3:77171852-77171871 Msa0474330:intron 65.0%
GCCTGCGGCAAAACACACCG+TGG + chr3_3:77172421-77172440 Msa0474330:CDS 65.0%
!! CTCGGGCTCTGGGTTGGGCT+TGG - chr3_3:77170253-77170272 None:intergenic 70.0%
GGAGGGTCCCTAACGGGGAG+TGG - chr3_3:77171850-77171869 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 77169792 77173624 77169792 ID=Msa0474330;Name=Msa0474330
chr3_3 mRNA 77169792 77173624 77169792 ID=Msa0474330-mRNA-1;Parent=Msa0474330;Name=Msa0474330-mRNA-1;_AED=0.01;_eAED=0.01;_QI=334|1|1|1|1|1|4|287|586
chr3_3 exon 77169792 77171367 77169792 ID=Msa0474330-mRNA-1:exon:20470;Parent=Msa0474330-mRNA-1
chr3_3 exon 77171615 77171739 77171615 ID=Msa0474330-mRNA-1:exon:20471;Parent=Msa0474330-mRNA-1
chr3_3 exon 77172325 77172460 77172325 ID=Msa0474330-mRNA-1:exon:20472;Parent=Msa0474330-mRNA-1
chr3_3 exon 77173080 77173624 77173080 ID=Msa0474330-mRNA-1:exon:20473;Parent=Msa0474330-mRNA-1
chr3_3 five_prime_UTR 77169792 77170125 77169792 ID=Msa0474330-mRNA-1:five_prime_utr;Parent=Msa0474330-mRNA-1
chr3_3 CDS 77170126 77171367 77170126 ID=Msa0474330-mRNA-1:cds;Parent=Msa0474330-mRNA-1
chr3_3 CDS 77171615 77171739 77171615 ID=Msa0474330-mRNA-1:cds;Parent=Msa0474330-mRNA-1
chr3_3 CDS 77172325 77172460 77172325 ID=Msa0474330-mRNA-1:cds;Parent=Msa0474330-mRNA-1
chr3_3 CDS 77173080 77173337 77173080 ID=Msa0474330-mRNA-1:cds;Parent=Msa0474330-mRNA-1
chr3_3 three_prime_UTR 77173338 77173624 77173338 ID=Msa0474330-mRNA-1:three_prime_utr;Parent=Msa0474330-mRNA-1
Gene Sequence

>Msa0474330

ATGGCTGAGGAATCTCCAAAGATAGCCACAACTCAAGAGGCAGTGCCTGTTGCTGAGGAGGAGCAAGTGGTGGTCACTCAAAAAACAGAATCCACTGTTCCAGCTGACAAAGAATCTGATGAGCCCAAGCCCAACCCAGAGCCCGAGAAAAAGCCCGTTGAAAACAACACTGCTGAGACCGAGGAGGTATCAAAGCCCAGTGGTGATGAAAAAATTCCAGAGTCTGGTTCGTTCAAGGAAGAGAGCACTATTGTTGGTGAGCTACCTGAAGCTGAGAAGAAAGCACTTACAGAGCTGAAACAGCTTATTCAAGAAGCACTTAACAAACATGAATTCTCTGCACCACCTTCAACTACTCCAAGCCCAGTTAAAGAACAAAAGCCCGAGCCCACACCAGAAGCAGAAGCAGAATCACAACCACCTGCTGAAGAGACCAACAAGAAAGATGAACAGAACAATGAACAGGTTTCTGAAACAGAATCTGTTGTTGCTGTTACTACTGATGAAGTATCAACACCACCACTACCAACAACAACAACTGAAGCTGAACAACAACCAAAAGAAGAAGCAGAAAAGAAAGAAACAGAGGTTGCTGCTTCTTCTGTAGATGAAGATGGAGCAAAAACAGTTGAAGCAATTGAAGAATCTGTTGTAGCTGTAGCTTCATCAGTTCCAGAGGAACCAAAGGTGGTGGAAGCTTCTTCACCGGAACAACAACAACAACAACCAGAAGAGGTTTCAATATGGGGGATACCACTTCTTGCAGACGATAGAACTGACGTGATTCTGTTAAAGTTTCTACGTGCCAGAGACTTCAAAGTGAAAGAAGCATTCACAATGATCAAGAACACTATCCGTTGGAGAAAAGAGTTCAAAATTGAAGAGTTATTATTGGATGAAAACTTAGGTGATGAATATTTAGAGAAAACTGTTTACATGCATGGATATGACAAAGAAGGACACCCTGTTTGTTATAACATTTATGGAGAGTTTGATAACAAGGAGGTTTATAAGAAAACATTTTCTGATGAAGAGAAGAGAGAAAAGTTCTTGAAATGGAGAATTCTGGTCTTGGAGAAGAGTATTAGGAAGTTGGATTTTACCCCTGGTGGTATTTGCACTATTGTTCAAGTTAATGATCTTAAGAATTCTCCTGGACCTACCAAATGGGAGCTTAGACAAGCTACCAAACAAGCCCTTCAATTGCTTCAAGATAATTATCCTGAATTTGTAGCCAAACAGGTGTTTATCAATGTGCCATGGTGGTACTTAGCCGTGAACAGAATGATAAGTCCTTTTCTTACACAAAGAACAAAAAGCAAGTTTGTATTTGCTGGACCTTCCAAATCAGCTGAGACTCTTCTAAGTTACATAGCTCCTGAGCAACTTTCAGTCAAATATGGTGGACTAAGCAAAGATGGTGAATTTGGAATTACGGATGCTGTTACAGAAATTACAGTGAGGCCTGCGGCAAAACACACCGTGGAATTTCCAGTTACTGAGAACTGTCTACTCTCATGGGAGCTAAGAGTAATTGGGTGGGACATAAGCTATGGTGCAGAGTTTGTACCAAGTTCAGAAGGAAGCTACACTGTGATAATCCAGAAGACTAGAAAAATTGCTTCATCAGAAGAACCAGTGCTTTGCAACAACTACAAAATTGGTGAACCTGGGAAAGTGGTTCTCACCATTGACAATTCAAGCTCTAAGAAGAAGAAGCTCTTGTACCGCTTGAAGACCAAGCCTTCAACTTCTGATTAA
Protein sequence

>Msa0474330

MAEESPKIATTQEAVPVAEEEQVVVTQKTESTVPADKESDEPKPNPEPEKKPVENNTAETEEVSKPSGDEKIPESGSFKEESTIVGELPEAEKKALTELKQLIQEALNKHEFSAPPSTTPSPVKEQKPEPTPEAEAESQPPAEETNKKDEQNNEQVSETESVVAVTTDEVSTPPLPTTTTEAEQQPKEEAEKKETEVAASSVDEDGAKTVEAIEESVVAVASSVPEEPKVVEASSPEQQQQQPEEVSIWGIPLLADDRTDVILLKFLRARDFKVKEAFTMIKNTIRWRKEFKIEELLLDENLGDEYLEKTVYMHGYDKEGHPVCYNIYGEFDNKEVYKKTFSDEEKREKFLKWRILVLEKSIRKLDFTPGGICTIVQVNDLKNSPGPTKWELRQATKQALQLLQDNYPEFVAKQVFINVPWWYLAVNRMISPFLTQRTKSKFVFAGPSKSAETLLSYIAPEQLSVKYGGLSKDGEFGITDAVTEITVRPAAKHTVEFPVTENCLLSWELRVIGWDISYGAEFVPSSEGSYTVIIQKTRKIASSEEPVLCNNYKIGEPGKVVLTIDNSSSKKKKLLYRLKTKPSTSD*