AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0518750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518750 MtrunA17_Chr3g0131071 91.186 590 31 7 1 586 1 573 0.0 1003
Msa0518750 MtrunA17_Chr8g0385291 70.876 491 119 10 112 586 21 503 0.0 668
Msa0518750 MtrunA17_Chr4g0014901 68.475 387 117 4 199 582 208 592 0.0 546
Msa0518750 MtrunA17_Chr4g0014911 63.081 344 120 2 244 582 121 462 1.94e-158 461
Msa0518750 MtrunA17_Chr2g0293201 54.913 346 146 6 245 583 124 466 6.99e-134 399
Msa0518750 MtrunA17_Chr4g0004981 51.045 335 155 6 245 574 107 437 7.14e-114 347
Msa0518750 MtrunA17_Chr1g0183881 41.379 348 191 7 240 581 124 464 4.85e-91 288
Msa0518750 MtrunA17_Chr7g0243541 41.618 346 148 9 247 586 100 397 8.15e-83 265
Msa0518750 MtrunA17_Chr3g0131581 30.476 210 132 4 260 469 67 262 1.10e-22 98.2
Msa0518750 MtrunA17_Chr8g0385681 30.476 210 132 5 260 469 50 245 1.91e-18 85.5
Msa0518750 MtrunA17_Chr8g0385691 30.000 210 133 5 260 469 47 242 2.57e-18 85.1
Msa0518750 MtrunA17_Chr7g0254621 28.112 249 150 9 235 474 16 244 4.05e-13 70.9
Msa0518750 MtrunA17_Chr3g0144911 28.378 222 134 8 262 469 110 320 1.44e-11 66.6
Msa0518750 MtrunA17_Chr5g0399151 27.948 229 140 8 263 477 116 333 1.88e-11 66.6
Msa0518750 MtrunA17_Chr4g0035391 27.477 222 136 8 262 469 107 317 2.00e-11 67.0
Msa0518750 MtrunA17_Chr1g0155521 27.727 220 138 8 262 469 112 322 4.56e-11 65.9
Msa0518750 MtrunA17_Chr8g0372651 27.149 221 138 8 262 469 79 289 8.60e-11 64.7
Msa0518750 MtrunA17_Chr3g0144891 27.027 222 137 8 262 469 97 307 9.74e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518750 AT4G09160.1 64.571 350 117 3 238 582 317 664 1.46e-169 497
Msa0518750 AT4G09160.2 64.571 350 117 3 238 582 292 639 1.48e-169 496
Msa0518750 AT4G09160.3 64.571 350 117 3 238 582 292 639 1.81e-169 496
Msa0518750 AT1G72160.1 54.244 483 193 10 116 582 19 489 5.59e-168 487
Msa0518750 AT1G22530.2 43.570 661 256 14 14 581 12 648 1.32e-150 448
Msa0518750 AT1G72150.1 51.606 498 220 10 90 581 91 573 2.79e-149 442
Msa0518750 AT1G22530.1 54.780 387 157 5 196 581 313 682 4.19e-144 432
Msa0518750 AT1G30690.1 41.455 550 267 16 51 585 30 539 3.40e-123 374
Msa0518750 AT1G30690.2 41.455 550 267 16 51 585 30 539 3.40e-123 374
Msa0518750 AT3G51670.1 46.174 379 193 8 213 585 34 407 6.64e-113 343
Msa0518750 AT1G01630.1 30.000 210 133 5 260 469 50 245 2.19e-19 88.6
Msa0518750 AT4G08690.2 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0518750 AT4G08690.1 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0518750 AT4G08690.3 26.721 247 159 7 237 478 17 246 1.77e-14 75.1
Msa0518750 AT3G24840.1 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0518750 AT3G24840.6 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0518750 AT3G24840.4 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0518750 AT3G24840.2 25.574 305 199 10 191 477 27 321 1.21e-12 71.2
Msa0518750 AT3G24840.7 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0518750 AT3G24840.3 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0518750 AT3G24840.5 25.574 305 199 10 191 477 27 321 1.32e-12 70.9
Msa0518750 AT4G34580.2 26.606 218 143 6 262 469 88 298 6.92e-12 68.6
Msa0518750 AT4G34580.1 26.606 218 143 6 262 469 88 298 6.92e-12 68.6
Msa0518750 AT1G19650.1 28.829 222 133 7 262 469 104 314 7.37e-12 68.6
Msa0518750 AT2G21540.6 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0518750 AT2G21540.2 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0518750 AT2G21540.1 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0518750 AT2G21540.4 28.511 235 137 11 251 469 86 305 1.17e-11 67.8
Msa0518750 AT2G21540.3 28.571 224 131 10 262 469 95 305 1.32e-11 67.8
Msa0518750 AT2G21540.5 28.571 224 131 10 262 469 95 305 1.32e-11 67.8
Msa0518750 AT4G39170.2 29.148 223 131 9 262 469 108 318 1.88e-11 67.4
Msa0518750 AT2G18180.2 26.957 230 146 9 253 469 71 291 1.94e-11 67.4
Msa0518750 AT4G39170.1 29.148 223 131 9 262 469 108 318 1.98e-11 67.4
Msa0518750 AT4G39180.3 27.273 231 145 8 251 469 87 306 3.02e-11 66.6
Msa0518750 AT4G39180.2 27.273 231 145 8 251 469 87 306 3.02e-11 66.6
Msa0518750 AT2G18180.1 26.957 230 146 9 253 469 71 291 3.04e-11 66.6
Msa0518750 AT4G39180.1 26.866 268 168 10 215 469 54 306 3.10e-11 66.6
Msa0518750 AT1G22180.2 25.896 251 161 6 235 478 18 250 3.27e-11 65.1
Msa0518750 AT1G22180.4 25.896 251 161 6 235 478 18 250 3.27e-11 65.1
Msa0518750 AT1G75170.2 24.889 225 128 6 259 469 43 240 5.19e-11 64.3
Msa0518750 AT1G75170.1 24.889 225 128 6 259 469 43 240 5.19e-11 64.3
Msa0518750 AT1G19650.3 28.829 222 133 7 262 469 104 314 7.06e-11 65.1

Find 168 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGGGCTCTGGGTTGGGCT+TGG 0.133515 3_4:-70585804 None:intergenic
TCATGGGAGCTAAGAGTAAT+TGG 0.180786 3_4:+70588154 Msa0518750:CDS
CCTGTTTGTTATAACATTTA+TGG 0.191442 3_4:+70586643 Msa0518750:CDS
ACCTGTTTGGCTACAAATTC+AGG 0.192401 3_4:-70586901 None:intergenic
TCAAATAACACCGTTATTTC+CGG 0.205164 3_4:+70585431 Msa0518750:five_prime_UTR
TGTGATTCTGCTTCTGCTTC+TGG 0.205662 3_4:-70586073 None:intergenic
ATTATATGCAGATGACTTAT+TGG 0.206380 3_4:+70588523 Msa0518750:three_prime_UTR
CAAAATTGAAGAGTTATTAT+TGG 0.213965 3_4:+70586552 Msa0518750:CDS
AATAACGATGAAAAGTATTT+TGG 0.229828 3_4:-70585628 None:intergenic
CTAAGCAAAGATGGTGAATT+TGG 0.229951 3_4:+70587427 Msa0518750:CDS
TCTTGAGTTGTGGCTATCTT+TGG 0.230004 3_4:-70585695 None:intergenic
CTTTGCAACAACTACAAAAT+TGG 0.236225 3_4:+70588280 Msa0518750:CDS
TCGGGCTCTGGGTTGGGCTT+GGG 0.258053 3_4:-70585803 None:intergenic
AAAAGCAAGTTTGTATTTGC+TGG 0.284258 3_4:+70587233 Msa0518750:CDS
ACCAAATTCACCTCAGTAAC+TGG 0.284996 3_4:-70587511 None:intergenic
TTCTGCTTCTGGTGTGGGCT+CGG 0.286758 3_4:-70586062 None:intergenic
GTTCTTGAAATGGAGAATTC+TGG 0.287184 3_4:+70586726 Msa0518750:CDS
GTTCTGTTCATCTTTCTTGT+TGG 0.294746 3_4:-70586113 None:intergenic
AGTGCTTTCTTCTCAGCTTC+AGG 0.302927 3_4:-70585944 None:intergenic
CGGGCCATGGACCCACTTAA+TGG 0.315039 3_4:+70585451 Msa0518750:five_prime_UTR
CCCCATATTGAAACCTCTTC+TGG 0.316394 3_4:-70586406 None:intergenic
TGGTCTTGGAGAAGAGTATT+AGG 0.324429 3_4:+70586746 Msa0518750:CDS
AGAGAGAAAAGTTCTTGAAA+TGG 0.327651 3_4:+70586716 Msa0518750:CDS
ACAAGCATATAGATCAAATT+AGG 0.328233 3_4:-70588588 None:intergenic
TCCACCACCTTTGGTTCCTC+TGG 0.337500 3_4:-70586352 None:intergenic
CCATAAATGTTATAACAAAC+AGG 0.338368 3_4:-70586643 None:intergenic
TAGAAGAGTCTCAGCTGATT+TGG 0.338628 3_4:-70587261 None:intergenic
CGTTGGCTTCAAATTCTAAC+AGG 0.344786 3_4:+70585362 Msa0518750:five_prime_UTR
GTCTGTGCCAGATTGGAATC+TGG 0.348569 3_4:-70585484 None:intergenic
TTCTTTCACTTTGAAGTCTC+TGG 0.352608 3_4:-70586485 None:intergenic
TTCTTGTTGGTCTCTTCAGC+AGG 0.368545 3_4:-70586100 None:intergenic
AACAGGCTGGGCTATGTAAC+TGG 0.372569 3_4:+70585379 Msa0518750:five_prime_UTR
GAGCAACTTTCAGTCAAATA+TGG 0.373495 3_4:+70587400 Msa0518750:CDS
GGAGAAGAGTATTAGGAAGT+TGG 0.378620 3_4:+70586753 Msa0518750:CDS
GTCTTAATCAAAATCAACTA+TGG 0.378649 3_4:+70585661 Msa0518750:exon
GAAGAAGCTTCCACCACCTT+TGG 0.382704 3_4:-70586361 None:intergenic
AACCAGAAGAGGTTTCAATA+TGG 0.384526 3_4:+70586404 Msa0518750:CDS
CTTTCTTAATCTGAAGTTGA+AGG 0.385634 3_4:-70588382 None:intergenic
AAGCAATTGAAGGGCTTGTT+TGG 0.391690 3_4:-70586866 None:intergenic
TCAGCTTCAGTTGTTGTTGT+TGG 0.393012 3_4:-70586205 None:intergenic
AATCAGCTGAGACTCTTCTA+AGG 0.396379 3_4:+70587264 Msa0518750:CDS
TCCTGAATTTGTAGCCAAAC+AGG 0.397889 3_4:+70586900 Msa0518750:CDS
GCTAGTAGGCCCTCCGTGTT+TGG 0.400172 3_4:+70585401 Msa0518750:five_prime_UTR
TCTGCTTCTGCTTCTGGTGT+GGG 0.403305 3_4:-70586067 None:intergenic
TCTGCTTCTGGTGTGGGCTC+GGG 0.406078 3_4:-70586061 None:intergenic
CAAATAACACCGTTATTTCC+GGG 0.410392 3_4:+70585432 Msa0518750:five_prime_UTR
AACTACAAAATTGGTGAACC+TGG 0.412559 3_4:+70588289 Msa0518750:CDS
GGCTTCAAATTCTAACAGGC+TGG 0.417907 3_4:+70585366 Msa0518750:five_prime_UTR
CTTAGAGCTTGAATTGTCAA+TGG 0.418583 3_4:-70588326 None:intergenic
GTAAAGTATTAGTACCTGTT+TGG 0.422219 3_4:-70586914 None:intergenic
TTTGTTCTTTGTGTAAGAAA+AGG 0.424626 3_4:-70587212 None:intergenic
ACTTGCTCCTCCTCAGCAAC+AGG 0.426842 3_4:-70585725 None:intergenic
TTAATCTGAAGTTGAAGGCT+TGG 0.430689 3_4:-70588377 None:intergenic
TCTGTGTCCCAATAGTAAAA+AGG 0.437051 3_4:+70588660 Msa0518750:three_prime_UTR
GAGAAAACTGTTTACATGCA+TGG 0.439519 3_4:+70586601 Msa0518750:CDS
TCTGTGCCAGATTGGAATCT+GGG 0.439720 3_4:-70585483 None:intergenic
GGATAGAGGGGTTGTTTCGT+GGG 0.440935 3_4:-70585545 None:intergenic
GATTGGAATCTGGGCTCAAC+AGG 0.442208 3_4:-70585474 None:intergenic
TCAGTTGTTGTTGTTGGTAG+TGG 0.442852 3_4:-70586199 None:intergenic
TTCTGCTTCTGCTTCTGGTG+TGG 0.443749 3_4:-70586068 None:intergenic
ACTGGGCTTGGAGTAGTTGA+AGG 0.447024 3_4:-70586025 None:intergenic
TTCTTTGTCAGCTGGAACAG+TGG 0.452483 3_4:-70585771 None:intergenic
TCCAGAGTCTGGTTCGTTCA+AGG 0.461611 3_4:+70585895 Msa0518750:CDS
AGCTTGTCTAAGCTCCCATT+TGG 0.463609 3_4:-70586842 None:intergenic
GTGTGATGGGGATAGGATAG+AGG 0.466128 3_4:-70585559 None:intergenic
ACCAGAAGAGGTTTCAATAT+GGG 0.467828 3_4:+70586405 Msa0518750:CDS
CATGGGAGCTAAGAGTAATT+GGG 0.468056 3_4:+70588155 Msa0518750:CDS
AATTATCTTGAAGCAATTGA+AGG 0.470059 3_4:-70586876 None:intergenic
GAAATGGAGAATTCTGGTCT+TGG 0.470951 3_4:+70586732 Msa0518750:CDS
ATTCTCCTGGACCTACCAAA+TGG 0.471318 3_4:+70586827 Msa0518750:CDS
AGGATAGAGGGGTTGTTTCG+TGG 0.476967 3_4:-70585546 None:intergenic
GTGTAGCTTCCTTCTGAACT+TGG 0.478836 3_4:-70588208 None:intergenic
AATGATCTTAAGAATTCTCC+TGG 0.479288 3_4:+70586814 Msa0518750:CDS
AGGACCAGTCTGTGCCAGAT+TGG 0.480098 3_4:-70585491 None:intergenic
AGCTCCCATTTGGTAGGTCC+AGG 0.482236 3_4:-70586832 None:intergenic
TTGTAGTTGTTGCAAAGCAC+TGG 0.484047 3_4:-70588274 None:intergenic
GGCAGTGCCTGTTGCTGAGG+AGG 0.486650 3_4:+70585718 Msa0518750:CDS
TCATCAGATTCTTTGTCAGC+TGG 0.490379 3_4:-70585779 None:intergenic
TTATTATTGGATGAAAACTT+AGG 0.493600 3_4:+70586565 Msa0518750:CDS
AAGGAAGAGAGCACTATTGT+TGG 0.493837 3_4:+70585914 Msa0518750:CDS
TGCAGAACTGTCTACTCTCA+TGG 0.494143 3_4:+70588137 Msa0518750:intron
GGGCTATGTAACTGGCTAGT+AGG 0.494657 3_4:+70585387 Msa0518750:five_prime_UTR
ACACCGTTATTTCCGGGCCA+TGG 0.497800 3_4:+70585438 Msa0518750:five_prime_UTR
AGATGAACAGAACAATGAAC+AGG 0.499148 3_4:+70586123 Msa0518750:CDS
TGTCTAAGCTCCCATTTGGT+AGG 0.502674 3_4:-70586838 None:intergenic
GGAGAGTGGTGTAGTGTGAT+GGG 0.505695 3_4:-70585572 None:intergenic
TTCTCCTGGACCTACCAAAT+GGG 0.508042 3_4:+70586828 Msa0518750:CDS
CATTAAGTGGGTCCATGGCC+CGG 0.511063 3_4:-70585450 None:intergenic
TATTTGACTGAAAGTTGCTC+AGG 0.515624 3_4:-70587397 None:intergenic
GGTCCATGGCCCGGAAATAA+CGG 0.519376 3_4:-70585441 None:intergenic
ATCAAAATCAACTATGGCTG+AGG 0.520145 3_4:+70585667 Msa0518750:exon
TATGGTGGACTAAGCAAAGA+TGG 0.520271 3_4:+70587418 Msa0518750:CDS
GCTTCTTCTGTAGATGAAGA+TGG 0.521848 3_4:+70586274 Msa0518750:CDS
GTTGTTGTTGTTGGTAGTGG+TGG 0.522925 3_4:-70586196 None:intergenic
AAAGAGTGGGGCGGATGAGA+AGG 0.524597 3_4:-70585511 None:intergenic
CTGTTAGAATTTGAAGCCAA+CGG 0.527127 3_4:-70585361 None:intergenic
TCCTTGAACGAACCAGACTC+TGG 0.528848 3_4:-70585896 None:intergenic
GTTGAGCCCAGATTCCAATC+TGG 0.532116 3_4:+70585477 Msa0518750:five_prime_UTR
TTGTTGGTCTCTTCAGCAGG+TGG 0.536894 3_4:-70586097 None:intergenic
GGGAAGCAGTTATAAAGAGT+GGG 0.537800 3_4:-70585524 None:intergenic
TCCAGAGGAACCAAAGGTGG+TGG 0.538723 3_4:+70586351 Msa0518750:CDS
ATGGTGAGAACAACTTTCCC+AGG 0.541945 3_4:-70588307 None:intergenic
CCAGAAGAGGTTTCAATATG+GGG 0.547282 3_4:+70586406 Msa0518750:CDS
GATTCCAATCTGGCACAGAC+TGG 0.548284 3_4:+70585487 Msa0518750:five_prime_UTR
CAGGCCATTAAGTGGGTCCA+TGG 0.548475 3_4:-70585455 None:intergenic
TGGAGAGTGGTGTAGTGTGA+TGG 0.549657 3_4:-70585573 None:intergenic
TGGTGTAGTGTGATGGGGAT+AGG 0.550513 3_4:-70585566 None:intergenic
GAGTTTGTACCAAGTTCAGA+AGG 0.553555 3_4:+70588199 Msa0518750:CDS
GCTTCAAATTCTAACAGGCT+GGG 0.554058 3_4:+70585367 Msa0518750:five_prime_UTR
ATTATCTTGAAGCAATTGAA+GGG 0.556517 3_4:-70586875 None:intergenic
GGGTTGGAAAGAAATGTGAA+TGG 0.560559 3_4:+70585336 None:intergenic
AGAAATGTGAATGGAACCGT+TGG 0.566113 3_4:+70585345 Msa0518750:five_prime_UTR
ACAACAACAACAACCAGAAG+AGG 0.568417 3_4:+70586393 Msa0518750:CDS
GGCTCAACAGGCCATTAAGT+GGG 0.569499 3_4:-70585462 None:intergenic
GGGCTCAACAGGCCATTAAG+TGG 0.571417 3_4:-70585463 None:intergenic
TGAAGGAGATTGATGGAGAG+TGG 0.575247 3_4:-70585586 None:intergenic
TTATGGAGAGTTTGATAACA+AGG 0.578141 3_4:+70586660 Msa0518750:CDS
ATGCATGGATATGACAAAGA+AGG 0.578689 3_4:+70586616 Msa0518750:CDS
AGAGGCAGTGCCTGTTGCTG+AGG 0.578794 3_4:+70585715 Msa0518750:CDS
CTCAGTAACTGGAAATTCCA+CGG 0.580825 3_4:-70587500 None:intergenic
GGGGAAGCAGTTATAAAGAG+TGG 0.582308 3_4:-70585525 None:intergenic
GATAGAGGGGTTGTTTCGTG+GGG 0.588228 3_4:-70585544 None:intergenic
TAATCGTTATGATCTTGTGT+TGG 0.590693 3_4:+70588414 Msa0518750:three_prime_UTR
GTTCTATCGTCTGCAAGAAG+TGG 0.593136 3_4:-70586433 None:intergenic
AAAGATAGCCACAACTCAAG+AGG 0.593760 3_4:+70585697 Msa0518750:CDS
GGTGGTGGAAGCTTCTTCAC+CGG 0.595988 3_4:+70586366 Msa0518750:CDS
ACTGGGCTTTGATACCTCCT+CGG 0.596592 3_4:-70585858 None:intergenic
TGTGATGGGGATAGGATAGA+GGG 0.596822 3_4:-70585558 None:intergenic
AGGCACTGCCTCTTGAGTTG+TGG 0.597680 3_4:-70585705 None:intergenic
ACTACAAAATTGGTGAACCT+GGG 0.600044 3_4:+70588290 Msa0518750:CDS
TGATCAAGAACACTATCCGT+TGG 0.601471 3_4:+70586518 Msa0518750:CDS
GCAGAACTGTCTACTCTCAT+GGG 0.603658 3_4:+70588138 Msa0518750:intron
TGTTGCTGAGGAGGAGCAAG+TGG 0.604048 3_4:+70585727 Msa0518750:CDS
ATTGGGTGGGACATAAGCTA+TGG 0.608625 3_4:+70588172 Msa0518750:CDS
AGCAGAAAAGAAAGAAACAG+AGG 0.609236 3_4:+70586246 Msa0518750:CDS
CAACTTTCAGTCAAATATGG+TGG 0.612687 3_4:+70587403 Msa0518750:CDS
ATCAGTTCCAGAGGAACCAA+AGG 0.614270 3_4:+70586345 Msa0518750:CDS
ACAATAGTGCAAATACCACC+AGG 0.619339 3_4:-70586784 None:intergenic
AAACAACACTGCTGAGACCG+AGG 0.619853 3_4:+70585841 Msa0518750:CDS
GGGAGCTAAGAGTAATTGGG+TGG 0.623613 3_4:+70588158 Msa0518750:CDS
AGTTCCAGAGGAACCAAAGG+TGG 0.628144 3_4:+70586348 Msa0518750:CDS
CATAAATGTTATAACAAACA+GGG 0.628980 3_4:-70586642 None:intergenic
ATTTGACTCTCTACCAAACA+CGG 0.631193 3_4:-70585414 None:intergenic
AGAGTCTCAGCTGATTTGGA+AGG 0.633487 3_4:-70587257 None:intergenic
GACTCTCTACCAAACACGGA+GGG 0.636640 3_4:-70585410 None:intergenic
TTGAAGGCTTGGTCTTCAAG+CGG 0.637531 3_4:-70588366 None:intergenic
AGGTGTTTATCAATGTGCCA+TGG 0.641137 3_4:+70587159 Msa0518750:intron
TGGAGAGTTTGATAACAAGG+AGG 0.651054 3_4:+70586663 Msa0518750:CDS
TGTAGCTTCATCAGTTCCAG+AGG 0.651074 3_4:+70586336 Msa0518750:CDS
CAGAAGAGGTTTCAATATGG+GGG 0.653215 3_4:+70586407 Msa0518750:CDS
GAGAGTGGTGTAGTGTGATG+GGG 0.658399 3_4:-70585571 None:intergenic
CGTGGAATTTCCAGTTACTG+AGG 0.660408 3_4:+70587501 Msa0518750:CDS
AGAAATTACAGTGAGGCCTG+CGG 0.663813 3_4:+70587468 Msa0518750:CDS
GGAGCTAAGAGTAATTGGGT+GGG 0.668364 3_4:+70588159 Msa0518750:CDS
TGCTGAGGAGGAGCAAGTGG+TGG 0.671033 3_4:+70585730 Msa0518750:CDS
AGCAGTTATAAAGAGTGGGG+CGG 0.687982 3_4:-70585520 None:intergenic
GTGATGGGGATAGGATAGAG+GGG 0.699837 3_4:-70585557 None:intergenic
AATAGTGCAAATACCACCAG+GGG 0.702016 3_4:-70586782 None:intergenic
CAATAGTGCAAATACCACCA+GGG 0.705753 3_4:-70586783 None:intergenic
TGTTTATCAATGTGCCATGG+TGG 0.706720 3_4:+70587162 Msa0518750:CDS
GGAAGCAGTTATAAAGAGTG+GGG 0.706820 3_4:-70585523 None:intergenic
GGGCTTGGAGTAGTTGAAGG+TGG 0.709064 3_4:-70586022 None:intergenic
TTCACAGCTAAGTACCACCA+TGG 0.716803 3_4:-70587176 None:intergenic
GCCTGCGGCAAAACACACCG+TGG 0.718457 3_4:+70587483 Msa0518750:CDS
TGACTCTCTACCAAACACGG+AGG 0.735360 3_4:-70585411 None:intergenic
CAACACTGCTGAGACCGAGG+AGG 0.737926 3_4:+70585844 Msa0518750:CDS
CTGTTACAGAAATTACAGTG+AGG 0.780489 3_4:+70587461 Msa0518750:CDS
GAGGAGGTATCAAAGCCCAG+TGG 0.800821 3_4:+70585860 Msa0518750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATAGTATTATCTATTT+CGG - chr3_4:70588067-70588086 None:intergenic 10.0%
!!! TAAATTTCTTTTGTAAATTT+TGG + chr3_4:70588560-70588579 Msa0518750:three_prime_UTR 10.0%
!!! TCACAAAAATTTTTAAATGA+AGG - chr3_4:70585606-70585625 None:intergenic 15.0%
!!! TTTATTTGATTTTGTTTTGT+AGG + chr3_4:70587139-70587158 Msa0518750:intron 15.0%
!!! ATGTTTCTTTGATATTTTAA+AGG + chr3_4:70588031-70588050 Msa0518750:intron 15.0%
!!! TGTTTCTTTGATATTTTAAA+GGG + chr3_4:70588032-70588051 Msa0518750:intron 15.0%
!!! AATAACGATGAAAAGTATTT+TGG - chr3_4:70585631-70585650 None:intergenic 20.0%
!! CAAAATTGAAGAGTTATTAT+TGG + chr3_4:70586552-70586571 Msa0518750:CDS 20.0%
!! TTATTATTGGATGAAAACTT+AGG + chr3_4:70586565-70586584 Msa0518750:CDS 20.0%
!! CATAAATGTTATAACAAACA+GGG - chr3_4:70586645-70586664 None:intergenic 20.0%
!! AAATTTAAGAACAACAATTG+TGG - chr3_4:70587049-70587068 None:intergenic 20.0%
!!! TAGTGTCTTAATTTGTTTTA+TGG + chr3_4:70587355-70587374 Msa0518750:intron 20.0%
!! ACAAATTGTTGCATAAAATA+AGG - chr3_4:70587689-70587708 None:intergenic 20.0%
!! AAGGAATCATAAAATAAACA+TGG + chr3_4:70587752-70587771 Msa0518750:intron 20.0%
!!! ATGACACATTAATTTTTTGA+GGG + chr3_4:70587883-70587902 Msa0518750:intron 20.0%
!!! TTCTTTTTGCTGTATTATTT+TGG + chr3_4:70588455-70588474 Msa0518750:three_prime_UTR 20.0%
!!! TATTTTGGTTATTACTATTG+AGG + chr3_4:70588470-70588489 Msa0518750:three_prime_UTR 20.0%
!!! ATTTTGGTTATTACTATTGA+GGG + chr3_4:70588471-70588490 Msa0518750:three_prime_UTR 20.0%
!!! ATTTCTTTTGTAAATTTTGG+TGG + chr3_4:70588563-70588582 Msa0518750:three_prime_UTR 20.0%
!!! TTTCTTTTGTAAATTTTGGT+GGG + chr3_4:70588564-70588583 Msa0518750:three_prime_UTR 20.0%
!! TTTTAAATGAAGGAGATTGA+TGG - chr3_4:70585596-70585615 None:intergenic 25.0%
! GTCTTAATCAAAATCAACTA+TGG + chr3_4:70585661-70585680 Msa0518750:exon 25.0%
! CCATAAATGTTATAACAAAC+AGG - chr3_4:70586646-70586665 None:intergenic 25.0%
!!! CCTGTTTGTTATAACATTTA+TGG + chr3_4:70586643-70586662 Msa0518750:CDS 25.0%
! ATTATCTTGAAGCAATTGAA+GGG - chr3_4:70586878-70586897 None:intergenic 25.0%
! AATTATCTTGAAGCAATTGA+AGG - chr3_4:70586879-70586898 None:intergenic 25.0%
! GCAATTAATATAACTGAAAC+TGG - chr3_4:70586957-70586976 None:intergenic 25.0%
! TTTGTTCTTTGTGTAAGAAA+AGG - chr3_4:70587215-70587234 None:intergenic 25.0%
! AATTAAGACACTAACAAACT+AGG - chr3_4:70587348-70587367 None:intergenic 25.0%
!!! TTTTTTAGATTGAGTATCCA+AGG + chr3_4:70587577-70587596 Msa0518750:intron 25.0%
!! TTTTTAGATTGAGTATCCAA+GGG + chr3_4:70587578-70587597 Msa0518750:intron 25.0%
! AATATAAATATGAAGACCAG+AGG - chr3_4:70587640-70587659 None:intergenic 25.0%
! TAAACAAACAAAAGAGGAAA+AGG - chr3_4:70587666-70587685 None:intergenic 25.0%
! ATAAGGTAAACAAACAAAAG+AGG - chr3_4:70587672-70587691 None:intergenic 25.0%
! CTCAAAAAATTAATGTGTCA+TGG - chr3_4:70587884-70587903 None:intergenic 25.0%
!!! CATGACACATTAATTTTTTG+AGG + chr3_4:70587882-70587901 Msa0518750:intron 25.0%
!!! TTAATTTTTTGAGGGTTCAA+AGG + chr3_4:70587891-70587910 Msa0518750:intron 25.0%
!!! ATGCATTGAAATTTTGTTGT+TGG + chr3_4:70588106-70588125 Msa0518750:intron 25.0%
!!! TGCATTGAAATTTTGTTGTT+GGG + chr3_4:70588107-70588126 Msa0518750:intron 25.0%
!!! TTTTGGTTATTACTATTGAG+GGG + chr3_4:70588472-70588491 Msa0518750:three_prime_UTR 25.0%
! ATTATATGCAGATGACTTAT+TGG + chr3_4:70588523-70588542 Msa0518750:three_prime_UTR 25.0%
!!! TTCTTTTGTAAATTTTGGTG+GGG + chr3_4:70588565-70588584 Msa0518750:three_prime_UTR 25.0%
! ACAAGCATATAGATCAAATT+AGG - chr3_4:70588591-70588610 None:intergenic 25.0%
!! CGTTTTTCCTTTTTACTATT+GGG - chr3_4:70588670-70588689 None:intergenic 25.0%
!!! TCGTTTTTCCTTTTTACTAT+TGG - chr3_4:70588671-70588690 None:intergenic 25.0%
! TCAAATAACACCGTTATTTC+CGG + chr3_4:70585431-70585450 Msa0518750:five_prime_UTR 30.0%
! TTCTTTTCTGCTTCTTCTTT+TGG - chr3_4:70586238-70586257 None:intergenic 30.0%
! TTTTGAACTCTTTTCTCCAA+CGG - chr3_4:70586537-70586556 None:intergenic 30.0%
! TTATGGAGAGTTTGATAACA+AGG + chr3_4:70586660-70586679 Msa0518750:CDS 30.0%
AGAGAGAAAAGTTCTTGAAA+TGG + chr3_4:70586716-70586735 Msa0518750:CDS 30.0%
AATGATCTTAAGAATTCTCC+TGG + chr3_4:70586814-70586833 Msa0518750:CDS 30.0%
! GTAAAGTATTAGTACCTGTT+TGG - chr3_4:70586917-70586936 None:intergenic 30.0%
AAAAGCAAGTTTGTATTTGC+TGG + chr3_4:70587233-70587252 Msa0518750:CDS 30.0%
AGATACTAGATGCTTTATCA+AGG + chr3_4:70587733-70587752 Msa0518750:intron 30.0%
!! ATGGTCATTTTGTTAAGTCA+TGG - chr3_4:70587865-70587884 None:intergenic 30.0%
! GTCTATGATTTTGCTAAACT+TGG - chr3_4:70587960-70587979 None:intergenic 30.0%
!! AAGCAATTTTTCTAGTCTTC+TGG - chr3_4:70588243-70588262 None:intergenic 30.0%
CTTTGCAACAACTACAAAAT+TGG + chr3_4:70588280-70588299 Msa0518750:CDS 30.0%
! CTTTCTTAATCTGAAGTTGA+AGG - chr3_4:70588385-70588404 None:intergenic 30.0%
TAATCGTTATGATCTTGTGT+TGG + chr3_4:70588414-70588433 Msa0518750:three_prime_UTR 30.0%
! CTGTTAGAATTTGAAGCCAA+CGG - chr3_4:70585364-70585383 None:intergenic 35.0%
ATTTGACTCTCTACCAAACA+CGG - chr3_4:70585417-70585436 None:intergenic 35.0%
! CAAATAACACCGTTATTTCC+GGG + chr3_4:70585432-70585451 Msa0518750:five_prime_UTR 35.0%
ATCAAAATCAACTATGGCTG+AGG + chr3_4:70585667-70585686 Msa0518750:exon 35.0%
!!! GGAATTTTTTCATCACCACT+GGG - chr3_4:70585878-70585897 None:intergenic 35.0%
!!! TGGAATTTTTTCATCACCAC+TGG - chr3_4:70585879-70585898 None:intergenic 35.0%
GATGAAAAAATTCCAGAGTC+TGG + chr3_4:70585884-70585903 Msa0518750:CDS 35.0%
! TTTTGTTCTTTAACTGGGCT+TGG - chr3_4:70586040-70586059 None:intergenic 35.0%
GTTCTGTTCATCTTTCTTGT+TGG - chr3_4:70586116-70586135 None:intergenic 35.0%
AGATGAACAGAACAATGAAC+AGG + chr3_4:70586123-70586142 Msa0518750:CDS 35.0%
AGCAGAAAAGAAAGAAACAG+AGG + chr3_4:70586246-70586265 Msa0518750:CDS 35.0%
AACCAGAAGAGGTTTCAATA+TGG + chr3_4:70586404-70586423 Msa0518750:CDS 35.0%
ACCAGAAGAGGTTTCAATAT+GGG + chr3_4:70586405-70586424 Msa0518750:CDS 35.0%
!! TTCTTTCACTTTGAAGTCTC+TGG - chr3_4:70586488-70586507 None:intergenic 35.0%
GAGAAAACTGTTTACATGCA+TGG + chr3_4:70586601-70586620 Msa0518750:CDS 35.0%
ATGCATGGATATGACAAAGA+AGG + chr3_4:70586616-70586635 Msa0518750:CDS 35.0%
GTTCTTGAAATGGAGAATTC+TGG + chr3_4:70586726-70586745 Msa0518750:CDS 35.0%
CTGATACCACTAAATAGATG+CGG + chr3_4:70587023-70587042 Msa0518750:intron 35.0%
TATTTGACTGAAAGTTGCTC+AGG - chr3_4:70587400-70587419 None:intergenic 35.0%
GAGCAACTTTCAGTCAAATA+TGG + chr3_4:70587400-70587419 Msa0518750:CDS 35.0%
CAACTTTCAGTCAAATATGG+TGG + chr3_4:70587403-70587422 Msa0518750:CDS 35.0%
CTAAGCAAAGATGGTGAATT+TGG + chr3_4:70587427-70587446 Msa0518750:CDS 35.0%
CTGTTACAGAAATTACAGTG+AGG + chr3_4:70587461-70587480 Msa0518750:CDS 35.0%
! TTACCTTTCTGTGTTTTGTC+AGG + chr3_4:70587545-70587564 Msa0518750:intron 35.0%
AATCCTGACAAAACACAGAA+AGG - chr3_4:70587551-70587570 None:intergenic 35.0%
! TGTGTTTTGTCAGGATTGAT+TGG + chr3_4:70587554-70587573 Msa0518750:intron 35.0%
TGGATTGTGTAATCTTTCCT+AGG + chr3_4:70587611-70587630 Msa0518750:intron 35.0%
ATCTAGTATCTCTCAAGTCA+GGG - chr3_4:70587724-70587743 None:intergenic 35.0%
GGAAACAGACATACTTCAAT+TGG + chr3_4:70587773-70587792 Msa0518750:intron 35.0%
! TGGCATTGTCATTGTGATTT+TGG - chr3_4:70587927-70587946 None:intergenic 35.0%
AACTACAAAATTGGTGAACC+TGG + chr3_4:70588289-70588308 Msa0518750:CDS 35.0%
ACTACAAAATTGGTGAACCT+GGG + chr3_4:70588290-70588309 Msa0518750:CDS 35.0%
CTTAGAGCTTGAATTGTCAA+TGG - chr3_4:70588329-70588348 None:intergenic 35.0%
! TTAATCTGAAGTTGAAGGCT+TGG - chr3_4:70588380-70588399 None:intergenic 35.0%
TCTGTGTCCCAATAGTAAAA+AGG + chr3_4:70588660-70588679 Msa0518750:three_prime_UTR 35.0%
AGAAATGTGAATGGAACCGT+TGG + chr3_4:70585345-70585364 Msa0518750:five_prime_UTR 40.0%
!! CGTTGGCTTCAAATTCTAAC+AGG + chr3_4:70585362-70585381 Msa0518750:five_prime_UTR 40.0%
GCTTCAAATTCTAACAGGCT+GGG + chr3_4:70585367-70585386 Msa0518750:five_prime_UTR 40.0%
GGAAGCAGTTATAAAGAGTG+GGG - chr3_4:70585526-70585545 None:intergenic 40.0%
GGGAAGCAGTTATAAAGAGT+GGG - chr3_4:70585527-70585546 None:intergenic 40.0%
! TCTTGAGTTGTGGCTATCTT+TGG - chr3_4:70585698-70585717 None:intergenic 40.0%
AAAGATAGCCACAACTCAAG+AGG + chr3_4:70585697-70585716 Msa0518750:CDS 40.0%
TCATCAGATTCTTTGTCAGC+TGG - chr3_4:70585782-70585801 None:intergenic 40.0%
!! TTTTCAACGGGCTTTTTCTC+GGG - chr3_4:70585824-70585843 None:intergenic 40.0%
!! GTTTTCAACGGGCTTTTTCT+CGG - chr3_4:70585825-70585844 None:intergenic 40.0%
!!! TCAGCAGTGTTGTTTTCAAC+GGG - chr3_4:70585836-70585855 None:intergenic 40.0%
!!! CTCAGCAGTGTTGTTTTCAA+CGG - chr3_4:70585837-70585856 None:intergenic 40.0%
!! AAGGAAGAGAGCACTATTGT+TGG + chr3_4:70585914-70585933 Msa0518750:CDS 40.0%
! CGGGCTTTTGTTCTTTAACT+GGG - chr3_4:70586045-70586064 None:intergenic 40.0%
! TCGGGCTTTTGTTCTTTAAC+TGG - chr3_4:70586046-70586065 None:intergenic 40.0%
! TCAGTTGTTGTTGTTGGTAG+TGG - chr3_4:70586202-70586221 None:intergenic 40.0%
! TCAGCTTCAGTTGTTGTTGT+TGG - chr3_4:70586208-70586227 None:intergenic 40.0%
GCTTCTTCTGTAGATGAAGA+TGG + chr3_4:70586274-70586293 Msa0518750:CDS 40.0%
! GGTTGTTGTTGTTGTTGTTC+CGG - chr3_4:70586388-70586407 None:intergenic 40.0%
ACAACAACAACAACCAGAAG+AGG + chr3_4:70586393-70586412 Msa0518750:CDS 40.0%
CCAGAAGAGGTTTCAATATG+GGG + chr3_4:70586406-70586425 Msa0518750:CDS 40.0%
CAGAAGAGGTTTCAATATGG+GGG + chr3_4:70586407-70586426 Msa0518750:CDS 40.0%
TGATCAAGAACACTATCCGT+TGG + chr3_4:70586518-70586537 Msa0518750:CDS 40.0%
! TGGAGAGTTTGATAACAAGG+AGG + chr3_4:70586663-70586682 Msa0518750:CDS 40.0%
GAAATGGAGAATTCTGGTCT+TGG + chr3_4:70586732-70586751 Msa0518750:CDS 40.0%
!! TGGTCTTGGAGAAGAGTATT+AGG + chr3_4:70586746-70586765 Msa0518750:CDS 40.0%
!! GGAGAAGAGTATTAGGAAGT+TGG + chr3_4:70586753-70586772 Msa0518750:CDS 40.0%
AATAGTGCAAATACCACCAG+GGG - chr3_4:70586785-70586804 None:intergenic 40.0%
CAATAGTGCAAATACCACCA+GGG - chr3_4:70586786-70586805 None:intergenic 40.0%
ACAATAGTGCAAATACCACC+AGG - chr3_4:70586787-70586806 None:intergenic 40.0%
! AAGCAATTGAAGGGCTTGTT+TGG - chr3_4:70586869-70586888 None:intergenic 40.0%
TCCTGAATTTGTAGCCAAAC+AGG + chr3_4:70586900-70586919 Msa0518750:CDS 40.0%
ACCTGTTTGGCTACAAATTC+AGG - chr3_4:70586904-70586923 None:intergenic 40.0%
CGCATCTATTTAGTGGTATC+AGG - chr3_4:70587025-70587044 None:intergenic 40.0%
AGGTGTTTATCAATGTGCCA+TGG + chr3_4:70587159-70587178 Msa0518750:intron 40.0%
TGTTTATCAATGTGCCATGG+TGG + chr3_4:70587162-70587181 Msa0518750:CDS 40.0%
TAGAAGAGTCTCAGCTGATT+TGG - chr3_4:70587264-70587283 None:intergenic 40.0%
AATCAGCTGAGACTCTTCTA+AGG + chr3_4:70587264-70587283 Msa0518750:CDS 40.0%
!! CTGAGACTCTTCTAAGGTAA+AGG + chr3_4:70587270-70587289 Msa0518750:intron 40.0%
!! TATGGTGGACTAAGCAAAGA+TGG + chr3_4:70587418-70587437 Msa0518750:CDS 40.0%
CTCAGTAACTGGAAATTCCA+CGG - chr3_4:70587503-70587522 None:intergenic 40.0%
TCCAGTTACTGAGGTGAATT+TGG + chr3_4:70587510-70587529 Msa0518750:intron 40.0%
ACCAAATTCACCTCAGTAAC+TGG - chr3_4:70587514-70587533 None:intergenic 40.0%
AATCTTTCCTAGGATTCCTC+TGG + chr3_4:70587621-70587640 Msa0518750:intron 40.0%
CATCTAGTATCTCTCAAGTC+AGG - chr3_4:70587725-70587744 None:intergenic 40.0%
AATGACAATGCCACGTGATT+TGG + chr3_4:70587934-70587953 Msa0518750:intron 40.0%
TCATGGGAGCTAAGAGTAAT+TGG + chr3_4:70588154-70588173 Msa0518750:CDS 40.0%
CATGGGAGCTAAGAGTAATT+GGG + chr3_4:70588155-70588174 Msa0518750:CDS 40.0%
GAGTTTGTACCAAGTTCAGA+AGG + chr3_4:70588199-70588218 Msa0518750:CDS 40.0%
TTGTAGTTGTTGCAAAGCAC+TGG - chr3_4:70588277-70588296 None:intergenic 40.0%
GGCTTCAAATTCTAACAGGC+TGG + chr3_4:70585366-70585385 Msa0518750:five_prime_UTR 45.0%
TCTGTGCCAGATTGGAATCT+GGG - chr3_4:70585486-70585505 None:intergenic 45.0%
AGCAGTTATAAAGAGTGGGG+CGG - chr3_4:70585523-70585542 None:intergenic 45.0%
GGGGAAGCAGTTATAAAGAG+TGG - chr3_4:70585528-70585547 None:intergenic 45.0%
! TGTGATGGGGATAGGATAGA+GGG - chr3_4:70585561-70585580 None:intergenic 45.0%
TGAAGGAGATTGATGGAGAG+TGG - chr3_4:70585589-70585608 None:intergenic 45.0%
TTCTTTGTCAGCTGGAACAG+TGG - chr3_4:70585774-70585793 None:intergenic 45.0%
AGTGCTTTCTTCTCAGCTTC+AGG - chr3_4:70585947-70585966 None:intergenic 45.0%
TGTGATTCTGCTTCTGCTTC+TGG - chr3_4:70586076-70586095 None:intergenic 45.0%
! TTCTTGTTGGTCTCTTCAGC+AGG - chr3_4:70586103-70586122 None:intergenic 45.0%
!! GTTGTTGTTGTTGGTAGTGG+TGG - chr3_4:70586199-70586218 None:intergenic 45.0%
TGTAGCTTCATCAGTTCCAG+AGG + chr3_4:70586336-70586355 Msa0518750:CDS 45.0%
ATCAGTTCCAGAGGAACCAA+AGG + chr3_4:70586345-70586364 Msa0518750:CDS 45.0%
CCCCATATTGAAACCTCTTC+TGG - chr3_4:70586409-70586428 None:intergenic 45.0%
GTTCTATCGTCTGCAAGAAG+TGG - chr3_4:70586436-70586455 None:intergenic 45.0%
! AGGAAGTTGGATTTTACCCC+TGG + chr3_4:70586766-70586785 Msa0518750:CDS 45.0%
! AAGTTGGATTTTACCCCTGG+TGG + chr3_4:70586769-70586788 Msa0518750:CDS 45.0%
ATTCTCCTGGACCTACCAAA+TGG + chr3_4:70586827-70586846 Msa0518750:CDS 45.0%
TTCTCCTGGACCTACCAAAT+GGG + chr3_4:70586828-70586847 Msa0518750:CDS 45.0%
TGTCTAAGCTCCCATTTGGT+AGG - chr3_4:70586841-70586860 None:intergenic 45.0%
AGCTTGTCTAAGCTCCCATT+TGG - chr3_4:70586845-70586864 None:intergenic 45.0%
TTGTGGCCGCATCTATTTAG+TGG - chr3_4:70587032-70587051 None:intergenic 45.0%
TTCACAGCTAAGTACCACCA+TGG - chr3_4:70587179-70587198 None:intergenic 45.0%
!! AGAGTCTCAGCTGATTTGGA+AGG - chr3_4:70587260-70587279 None:intergenic 45.0%
AGAAATTACAGTGAGGCCTG+CGG + chr3_4:70587468-70587487 Msa0518750:CDS 45.0%
CGTGGAATTTCCAGTTACTG+AGG + chr3_4:70587501-70587520 Msa0518750:CDS 45.0%
TATCCAAGGGAACGTTGCAT+TGG + chr3_4:70587591-70587610 Msa0518750:intron 45.0%
AATCCAATGCAACGTTCCCT+TGG - chr3_4:70587597-70587616 None:intergenic 45.0%
ATGAAGACCAGAGGAATCCT+AGG - chr3_4:70587631-70587650 None:intergenic 45.0%
TATCTCTCAAGTCAGGGCAT+AGG - chr3_4:70587718-70587737 None:intergenic 45.0%
CTAAACTTGGCCAAATCACG+TGG - chr3_4:70587947-70587966 None:intergenic 45.0%
TGCAGAACTGTCTACTCTCA+TGG + chr3_4:70588137-70588156 Msa0518750:intron 45.0%
GCAGAACTGTCTACTCTCAT+GGG + chr3_4:70588138-70588157 Msa0518750:intron 45.0%
GGAGCTAAGAGTAATTGGGT+GGG + chr3_4:70588159-70588178 Msa0518750:CDS 45.0%
ATTGGGTGGGACATAAGCTA+TGG + chr3_4:70588172-70588191 Msa0518750:CDS 45.0%
GTGTAGCTTCCTTCTGAACT+TGG - chr3_4:70588211-70588230 None:intergenic 45.0%
ATGGTGAGAACAACTTTCCC+AGG - chr3_4:70588310-70588329 None:intergenic 45.0%
! TTGAAGGCTTGGTCTTCAAG+CGG - chr3_4:70588369-70588388 None:intergenic 45.0%
AACAGGCTGGGCTATGTAAC+TGG + chr3_4:70585379-70585398 Msa0518750:five_prime_UTR 50.0%
GGGCTATGTAACTGGCTAGT+AGG + chr3_4:70585387-70585406 Msa0518750:five_prime_UTR 50.0%
GACTCTCTACCAAACACGGA+GGG - chr3_4:70585413-70585432 None:intergenic 50.0%
TGACTCTCTACCAAACACGG+AGG - chr3_4:70585414-70585433 None:intergenic 50.0%
GGCTCAACAGGCCATTAAGT+GGG - chr3_4:70585465-70585484 None:intergenic 50.0%
GATTGGAATCTGGGCTCAAC+AGG - chr3_4:70585477-70585496 None:intergenic 50.0%
GTTGAGCCCAGATTCCAATC+TGG + chr3_4:70585477-70585496 Msa0518750:five_prime_UTR 50.0%
GTCTGTGCCAGATTGGAATC+TGG - chr3_4:70585487-70585506 None:intergenic 50.0%
GATTCCAATCTGGCACAGAC+TGG + chr3_4:70585487-70585506 Msa0518750:five_prime_UTR 50.0%
! GATAGAGGGGTTGTTTCGTG+GGG - chr3_4:70585547-70585566 None:intergenic 50.0%
! GGATAGAGGGGTTGTTTCGT+GGG - chr3_4:70585548-70585567 None:intergenic 50.0%
! AGGATAGAGGGGTTGTTTCG+TGG - chr3_4:70585549-70585568 None:intergenic 50.0%
! GTGATGGGGATAGGATAGAG+GGG - chr3_4:70585560-70585579 None:intergenic 50.0%
! GTGTGATGGGGATAGGATAG+AGG - chr3_4:70585562-70585581 None:intergenic 50.0%
! TGGTGTAGTGTGATGGGGAT+AGG - chr3_4:70585569-70585588 None:intergenic 50.0%
GAGAGTGGTGTAGTGTGATG+GGG - chr3_4:70585574-70585593 None:intergenic 50.0%
GGAGAGTGGTGTAGTGTGAT+GGG - chr3_4:70585575-70585594 None:intergenic 50.0%
TGGAGAGTGGTGTAGTGTGA+TGG - chr3_4:70585576-70585595 None:intergenic 50.0%
!!! TTTTTCTCGGGCTCTGGGTT+GGG - chr3_4:70585812-70585831 None:intergenic 50.0%
AAACAACACTGCTGAGACCG+AGG + chr3_4:70585841-70585860 Msa0518750:CDS 50.0%
! ACTGGGCTTTGATACCTCCT+CGG - chr3_4:70585861-70585880 None:intergenic 50.0%
! TCCAGAGTCTGGTTCGTTCA+AGG + chr3_4:70585895-70585914 Msa0518750:CDS 50.0%
TCCTTGAACGAACCAGACTC+TGG - chr3_4:70585899-70585918 None:intergenic 50.0%
ACTGGGCTTGGAGTAGTTGA+AGG - chr3_4:70586028-70586047 None:intergenic 50.0%
TCTGCTTCTGCTTCTGGTGT+GGG - chr3_4:70586070-70586089 None:intergenic 50.0%
TTCTGCTTCTGCTTCTGGTG+TGG - chr3_4:70586071-70586090 None:intergenic 50.0%
! TTGTTGGTCTCTTCAGCAGG+TGG - chr3_4:70586100-70586119 None:intergenic 50.0%
AGTTCCAGAGGAACCAAAGG+TGG + chr3_4:70586348-70586367 Msa0518750:CDS 50.0%
GAAGAAGCTTCCACCACCTT+TGG - chr3_4:70586364-70586383 None:intergenic 50.0%
GGGAGCTAAGAGTAATTGGG+TGG + chr3_4:70588158-70588177 Msa0518750:CDS 50.0%
! ACACCGTTATTTCCGGGCCA+TGG + chr3_4:70585438-70585457 Msa0518750:five_prime_UTR 55.0%
GGTCCATGGCCCGGAAATAA+CGG - chr3_4:70585444-70585463 None:intergenic 55.0%
CATTAAGTGGGTCCATGGCC+CGG - chr3_4:70585453-70585472 None:intergenic 55.0%
CAGGCCATTAAGTGGGTCCA+TGG - chr3_4:70585458-70585477 None:intergenic 55.0%
GGGCTCAACAGGCCATTAAG+TGG - chr3_4:70585466-70585485 None:intergenic 55.0%
AGGACCAGTCTGTGCCAGAT+TGG - chr3_4:70585494-70585513 None:intergenic 55.0%
AAAGAGTGGGGCGGATGAGA+AGG - chr3_4:70585514-70585533 None:intergenic 55.0%
! AGGCACTGCCTCTTGAGTTG+TGG - chr3_4:70585708-70585727 None:intergenic 55.0%
ACTTGCTCCTCCTCAGCAAC+AGG - chr3_4:70585728-70585747 None:intergenic 55.0%
TGTTGCTGAGGAGGAGCAAG+TGG + chr3_4:70585727-70585746 Msa0518750:CDS 55.0%
!!! CTTTTTCTCGGGCTCTGGGT+TGG - chr3_4:70585813-70585832 None:intergenic 55.0%
!! GAGGAGGTATCAAAGCCCAG+TGG + chr3_4:70585860-70585879 Msa0518750:CDS 55.0%
GGGCTTGGAGTAGTTGAAGG+TGG - chr3_4:70586025-70586044 None:intergenic 55.0%
TTCTGCTTCTGGTGTGGGCT+CGG - chr3_4:70586065-70586084 None:intergenic 55.0%
TCCAGAGGAACCAAAGGTGG+TGG + chr3_4:70586351-70586370 Msa0518750:CDS 55.0%
! TCCACCACCTTTGGTTCCTC+TGG - chr3_4:70586355-70586374 None:intergenic 55.0%
!! GGTGGTGGAAGCTTCTTCAC+CGG + chr3_4:70586366-70586385 Msa0518750:CDS 55.0%
AGCTCCCATTTGGTAGGTCC+AGG - chr3_4:70586835-70586854 None:intergenic 55.0%
GCTAGTAGGCCCTCCGTGTT+TGG + chr3_4:70585401-70585420 Msa0518750:five_prime_UTR 60.0%
CGGGCCATGGACCCACTTAA+TGG + chr3_4:70585451-70585470 Msa0518750:five_prime_UTR 60.0%
AGAGGCAGTGCCTGTTGCTG+AGG + chr3_4:70585715-70585734 Msa0518750:CDS 60.0%
TGCTGAGGAGGAGCAAGTGG+TGG + chr3_4:70585730-70585749 Msa0518750:CDS 60.0%
!! CGGGCTTTTTCTCGGGCTCT+GGG - chr3_4:70585817-70585836 None:intergenic 60.0%
!! ACGGGCTTTTTCTCGGGCTC+TGG - chr3_4:70585818-70585837 None:intergenic 60.0%
CAACACTGCTGAGACCGAGG+AGG + chr3_4:70585844-70585863 Msa0518750:CDS 60.0%
TCTGCTTCTGGTGTGGGCTC+GGG - chr3_4:70586064-70586083 None:intergenic 60.0%
! TCCACGGTGTGTTTTGCCGC+AGG - chr3_4:70587487-70587506 None:intergenic 60.0%
GGCAGTGCCTGTTGCTGAGG+AGG + chr3_4:70585718-70585737 Msa0518750:CDS 65.0%
!! TCGGGCTCTGGGTTGGGCTT+GGG - chr3_4:70585806-70585825 None:intergenic 65.0%
GCCTGCGGCAAAACACACCG+TGG + chr3_4:70587483-70587502 Msa0518750:CDS 65.0%
!! CTCGGGCTCTGGGTTGGGCT+TGG - chr3_4:70585807-70585826 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 70585344 70588709 70585344 ID=Msa0518750;Name=Msa0518750
chr3_4 mRNA 70585344 70588709 70585344 ID=Msa0518750-mRNA-1;Parent=Msa0518750;Name=Msa0518750-mRNA-1;_AED=0.02;_eAED=0.02;_QI=336|1|1|1|1|1|4|310|586
chr3_4 exon 70585344 70586921 70585344 ID=Msa0518750-mRNA-1:exon:19163;Parent=Msa0518750-mRNA-1
chr3_4 exon 70587161 70587285 70587161 ID=Msa0518750-mRNA-1:exon:19164;Parent=Msa0518750-mRNA-1
chr3_4 exon 70587387 70587522 70587387 ID=Msa0518750-mRNA-1:exon:19165;Parent=Msa0518750-mRNA-1
chr3_4 exon 70588142 70588709 70588142 ID=Msa0518750-mRNA-1:exon:19166;Parent=Msa0518750-mRNA-1
chr3_4 five_prime_UTR 70585344 70585679 70585344 ID=Msa0518750-mRNA-1:five_prime_utr;Parent=Msa0518750-mRNA-1
chr3_4 CDS 70585680 70586921 70585680 ID=Msa0518750-mRNA-1:cds;Parent=Msa0518750-mRNA-1
chr3_4 CDS 70587161 70587285 70587161 ID=Msa0518750-mRNA-1:cds;Parent=Msa0518750-mRNA-1
chr3_4 CDS 70587387 70587522 70587387 ID=Msa0518750-mRNA-1:cds;Parent=Msa0518750-mRNA-1
chr3_4 CDS 70588142 70588399 70588142 ID=Msa0518750-mRNA-1:cds;Parent=Msa0518750-mRNA-1
chr3_4 three_prime_UTR 70588400 70588709 70588400 ID=Msa0518750-mRNA-1:three_prime_utr;Parent=Msa0518750-mRNA-1
Gene Sequence

>Msa0518750

ATGGCTGAGGAATCTCCAAAGATAGCCACAACTCAAGAGGCAGTGCCTGTTGCTGAGGAGGAGCAAGTGGTGGTCACTCAAAAAACAGAATCCACTGTTCCAGCTGACAAAGAATCTGATGAGCCCAAGCCCAACCCAGAGCCCGAGAAAAAGCCCGTTGAAAACAACACTGCTGAGACCGAGGAGGTATCAAAGCCCAGTGGTGATGAAAAAATTCCAGAGTCTGGTTCGTTCAAGGAAGAGAGCACTATTGTTGGTGAGCTACCTGAAGCTGAGAAGAAAGCACTTACAGAGCTGAAACAGCTTATTCAAGAAGCACTTAACAAACATGAATTCTCTGCACCACCTTCAACTACTCCAAGCCCAGTTAAAGAACAAAAGCCCGAGCCCACACCAGAAGCAGAAGCAGAATCACAACCACCTGCTGAAGAGACCAACAAGAAAGATGAACAGAACAATGAACAGGTTTCTGAAACAGAATCTGTTGTTGCTGTTACTACTGATGAAGTATCAACACCACCACTACCAACAACAACAACTGAAGCTGAACAACAACCAAAAGAAGAAGCAGAAAAGAAAGAAACAGAGGTTGCTGCTTCTTCTGTAGATGAAGATGGAGCAAAAACAGTTGAAGCAATTGAAGAATCTGTTGTAGCTGTAGCTTCATCAGTTCCAGAGGAACCAAAGGTGGTGGAAGCTTCTTCACCGGAACAACAACAACAACAACCAGAAGAGGTTTCAATATGGGGGATACCACTTCTTGCAGACGATAGAACTGACGTGATTCTGTTAAAGTTTCTACGTGCCAGAGACTTCAAAGTGAAAGAAGCATTCACAATGATCAAGAACACTATCCGTTGGAGAAAAGAGTTCAAAATTGAAGAGTTATTATTGGATGAAAACTTAGGTGATGAATATTTAGAGAAAACTGTTTACATGCATGGATATGACAAAGAAGGACACCCTGTTTGTTATAACATTTATGGAGAGTTTGATAACAAGGAGGTTTATAAGAAAACATTTTCTGATGAAGAGAAGAGAGAAAAGTTCTTGAAATGGAGAATTCTGGTCTTGGAGAAGAGTATTAGGAAGTTGGATTTTACCCCTGGTGGTATTTGCACTATTGTTCAAGTTAATGATCTTAAGAATTCTCCTGGACCTACCAAATGGGAGCTTAGACAAGCTACCAAACAAGCCCTTCAATTGCTTCAAGATAATTATCCTGAATTTGTAGCCAAACAGGTGTTTATCAATGTGCCATGGTGGTACTTAGCTGTGAACAGAATGATAAGTCCTTTTCTTACACAAAGAACAAAAAGCAAGTTTGTATTTGCTGGACCTTCCAAATCAGCTGAGACTCTTCTAAGTTACATAGCTCCTGAGCAACTTTCAGTCAAATATGGTGGACTAAGCAAAGATGGTGAATTTGGAATTACTGATGCTGTTACAGAAATTACAGTGAGGCCTGCGGCAAAACACACCGTGGAATTTCCAGTTACTGAGAACTGTCTACTCTCATGGGAGCTAAGAGTAATTGGGTGGGACATAAGCTATGGTGCAGAGTTTGTACCAAGTTCAGAAGGAAGCTACACTGTTATAATCCAGAAGACTAGAAAAATTGCTTCATCAGAAGAACCAGTGCTTTGCAACAACTACAAAATTGGTGAACCTGGGAAAGTTGTTCTCACCATTGACAATTCAAGCTCTAAGAAGAAGAAGCTCTTGTACCGCTTGAAGACCAAGCCTTCAACTTCAGATTAA
Protein sequence

>Msa0518750

MAEESPKIATTQEAVPVAEEEQVVVTQKTESTVPADKESDEPKPNPEPEKKPVENNTAETEEVSKPSGDEKIPESGSFKEESTIVGELPEAEKKALTELKQLIQEALNKHEFSAPPSTTPSPVKEQKPEPTPEAEAESQPPAEETNKKDEQNNEQVSETESVVAVTTDEVSTPPLPTTTTEAEQQPKEEAEKKETEVAASSVDEDGAKTVEAIEESVVAVASSVPEEPKVVEASSPEQQQQQPEEVSIWGIPLLADDRTDVILLKFLRARDFKVKEAFTMIKNTIRWRKEFKIEELLLDENLGDEYLEKTVYMHGYDKEGHPVCYNIYGEFDNKEVYKKTFSDEEKREKFLKWRILVLEKSIRKLDFTPGGICTIVQVNDLKNSPGPTKWELRQATKQALQLLQDNYPEFVAKQVFINVPWWYLAVNRMISPFLTQRTKSKFVFAGPSKSAETLLSYIAPEQLSVKYGGLSKDGEFGITDAVTEITVRPAAKHTVEFPVTENCLLSWELRVIGWDISYGAEFVPSSEGSYTVIIQKTRKIASSEEPVLCNNYKIGEPGKVVLTIDNSSSKKKKLLYRLKTKPSTSD*