AlfalfaGEDB | Msa0478630

M. sativa cultivar zhongmu-4 / Msa0478630

KEGG: K19202
GO: -
PFAM: PF13867.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478630	XP_013462103.1	98.701	231	2	1	1	230	1	231	2.33e-165	465

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478630	A0A072V439	98.701	231	2	1	1	230	1	231	1.11e-165	465

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0346740	Msa0478630	0.802039	6.776864e-49	-8.615850e-47
Msa0384370	Msa0478630	0.913552	5.390203e-84	-8.615850e-47
Msa0430370	Msa0478630	0.925644	1.387021e-90	-8.615850e-47
Msa0478630	Msa0522700	0.930996	7.246697e-94	-8.615850e-47
Msa0478630	Msa0563940	0.807945	3.944071e-50	-8.615850e-47
Msa0478630	Msa0625340	0.810220	1.284620e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478630	MtrunA17_Chr3g0141391	98.701	231	2	1	1	230	1	231	2.14e-169	465
Msa0478630	MtrunA17_Chr1g0154621	83.550	231	32	4	1	230	1	226	1.33e-136	382

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0478630	AT1G19330.2	76.522	230	51	3	1	230	1	227	6.26e-111	318
Msa0478630	AT1G19330.1	75.319	235	50	4	1	230	1	232	3.24e-109	314
Msa0478630	AT1G19330.3	75.000	236	50	5	1	230	1	233	5.65e-108	311
Msa0478630	AT1G75060.1	69.787	235	64	5	1	230	1	233	2.50e-106	306
Msa0478630	AT1G75060.2	69.362	235	64	6	1	230	1	232	8.14e-104	300
Msa0478630	AT1G75060.3	70.370	189	50	4	1	184	1	188	1.03e-80	240

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Find 118 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATAGCGGAGAAAATG+GGG	0.534955	3_3:+83473415	None:intergenic
AAACCAACCACGCAAACGCA+AGG	0.625045	3_3:-83473844	None:intergenic
AAAGATAGCGGAGAAAATGG+GGG	0.664645	3_3:+83473416	None:intergenic
AACATAGTAAGTACGAAGGG+AGG	0.698574	3_3:+83470393	None:intergenic
AAGAAAGCTGTTGGATTAGG+TGG	0.556533	3_3:-83473124	Msa0478630:CDS
AAGAAGAGAGAGAAAGTGAG+AGG	0.587528	3_3:+83473489	None:intergenic
AAGCTGTTGGATTAGGTGGT+TGG	0.508473	3_3:-83473120	Msa0478630:CDS
AATGAGTGACACGTAAGATT+AGG	0.462750	3_3:+83473580	None:intergenic
AATTACTAATATATACAATA+AGG	0.444214	3_3:+83470161	None:intergenic
ACAACAACCTTAGTATGACG+AGG	0.714849	3_3:+83473199	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAACACATATTTGTTTGC+TGG	+	chr3_3:83470050-83470069	None:intergenic	30.0%
TGGAAATAACCGTACAAAAT+CGG	-	chr3_3:83470647-83470666	Msa0478630:intron	30.0%
TGCATGTTTCTCTTTATTAC+TGG	-	chr3_3:83471158-83471177	Msa0478630:CDS	30.0%
TTTCTCTTTATTACTGGATC+TGG	-	chr3_3:83471164-83471183	Msa0478630:CDS	30.0%
AAAATAATACCAAAGGAGGT+AGG	+	chr3_3:83471285-83471304	None:intergenic	30.0%
TTGGAAAATAATACCAAAGG+AGG	+	chr3_3:83471289-83471308	None:intergenic	30.0%
ATAATGCAGGTTCTTACTAA+TGG	-	chr3_3:83471595-83471614	Msa0478630:CDS	30.0%
ATTGCACGTTATCAAATTCA+AGG	+	chr3_3:83471693-83471712	None:intergenic	30.0%
AAAATTATCTAATGCGTCAG+TGG	+	chr3_3:83472407-83472426	None:intergenic	30.0%
GAGGGAGTAATTTACAAATT+CGG	+	chr3_3:83472877-83472896	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	83469987	83473855	83469987	ID=Msa0478630;Name=Msa0478630
chr3_3	mRNA	83469987	83473855	83469987	ID=Msa0478630-mRNA-1;Parent=Msa0478630;Name=Msa0478630-mRNA-1;_AED=0.12;_eAED=0.12;_QI=526\|1\|1\|1\|1\|1\|6\|509\|230
chr3_3	exon	83469987	83470567	83469987	ID=Msa0478630-mRNA-1:exon:23443;Parent=Msa0478630-mRNA-1
chr3_3	exon	83471118	83471189	83471118	ID=Msa0478630-mRNA-1:exon:23442;Parent=Msa0478630-mRNA-1
chr3_3	exon	83471301	83471363	83471301	ID=Msa0478630-mRNA-1:exon:23441;Parent=Msa0478630-mRNA-1
chr3_3	exon	83471479	83471618	83471479	ID=Msa0478630-mRNA-1:exon:23440;Parent=Msa0478630-mRNA-1
chr3_3	exon	83472112	83472238	83472112	ID=Msa0478630-mRNA-1:exon:23439;Parent=Msa0478630-mRNA-1
chr3_3	exon	83473111	83473855	83473111	ID=Msa0478630-mRNA-1:exon:23438;Parent=Msa0478630-mRNA-1
chr3_3	five_prime_UTR	83473330	83473855	83473330	ID=Msa0478630-mRNA-1:five_prime_utr;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83473111	83473329	83473111	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83472112	83472238	83472112	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83471479	83471618	83471479	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83471301	83471363	83471301	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83471118	83471189	83471118	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	CDS	83470496	83470567	83470496	ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3	three_prime_UTR	83469987	83470495	83469987	ID=Msa0478630-mRNA-1:three_prime_utr;Parent=Msa0478630-mRNA-1

Gene Sequence

>Msa0478630

ATGATTGAAGCAATGGAAAGTTCAGTTAACGGTGGTGGTGGTTTCGCGACTCATTTGCAAAGCTTTGGTGGTGGTGGTGATAGTAGTGAAGAGGAACTGTCTGTGTTACCTCGTCATACTAAGGTTGTTGTTACTGGAAATAACCGTACAAAATCGGTTCTTGTTGGTCTTCAAGGCGTTGTTAAGAAAGCTGTTGGATTAGGTGGTTGGCATTGGCTGGTTCTTACTAATGGAATTGAAGTGAAGCTACAAAGGAATGCTCTTAGCGTGATTGAAGCACCCACTGGCAATGAAGAAGATGATGACCTTGAATTTGATAACGTGCAATGGAATGGATCAGATATGGCATCAGACGATACACACAAGTCTCACAAATCGAGGCACAGAATGCATAGATCATTGGGATCATGTCACAAGACCATGAGTAGGTCCTTCTCTGGTGATTCACAGTCTAAGGGGTCCGTTACTATGCCTCATGGGTCCATGAAGGTTGACTTGAGCAAGCTTGAAATGGCTGCATTGTGGAGATATTGGAGACATTTTAATCTTGTGGATGTCGTCCCAAACCCATCAAAGGAGCAGCTAGTAGATGTTGTTCAGAGGCATTTCATGTCCCAGCTGCAAATGGACGAGTTGCAGGTCATTAAGGGATTTGTCAATGCTGCAAAGAGGGTCAAGACTGTGTGCAAATGA

Protein sequence

>Msa0478630

MIEAMESSVNGGGGFATHLQSFGGGGDSSEEELSVLPRHTKVVVTGNNRTKSVLVGLQGVVKKAVGLGGWHWLVLTNGIEVKLQRNALSVIEAPTGNEEDDDLEFDNVQWNGSDMASDDTHKSHKSRHRMHRSLGSCHKTMSRSFSGDSQSKGSVTMPHGSMKVDLSKLEMAALWRYWRHFNLVDVVPNPSKEQLVDVVQRHFMSQLQMDELQVIKGFVNAAKRVKTVCK*