AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0478630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0478630 MtrunA17_Chr3g0141391 98.701 231 2 1 1 230 1 231 2.14e-169 465
Msa0478630 MtrunA17_Chr1g0154621 83.550 231 32 4 1 230 1 226 1.33e-136 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0478630 AT1G19330.2 76.522 230 51 3 1 230 1 227 6.26e-111 318
Msa0478630 AT1G19330.1 75.319 235 50 4 1 230 1 232 3.24e-109 314
Msa0478630 AT1G19330.3 75.000 236 50 5 1 230 1 233 5.65e-108 311
Msa0478630 AT1G75060.1 69.787 235 64 5 1 230 1 233 2.50e-106 306
Msa0478630 AT1G75060.2 69.362 235 64 6 1 230 1 232 8.14e-104 300
Msa0478630 AT1G75060.3 70.370 189 50 4 1 184 1 188 1.03e-80 240

Find 118 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGTAGGTTAATAGTTTA+AGG 0.109374 3_3:-83470336 Msa0478630:three_prime_UTR
CATACTAAGGTTGTTGTTAC+TGG 0.194013 3_3:-83473193 Msa0478630:CDS
AGCTGCTCCTTTGATGGGTT+TGG 0.231841 3_3:+83471155 None:intergenic
CTCTGGTGATTCACAGTCTA+AGG 0.252293 3_3:-83471508 Msa0478630:CDS
GGACGAGTTGCAGGTCATTA+AGG 0.256826 3_3:-83470540 Msa0478630:CDS
TTACTATGTTACATATTAAT+CGG 0.270714 3_3:-83470380 Msa0478630:three_prime_UTR
ACATATATCTACATAATAAC+TGG 0.282748 3_3:+83470280 None:intergenic
TGAAAAGATAGCGGAGAAAA+TGG 0.292286 3_3:+83473413 None:intergenic
TTGTTGTTATCAAAAGTATT+AGG 0.312640 3_3:-83469994 Msa0478630:three_prime_UTR
GTTAAGAAAGCTGTTGGATT+AGG 0.325633 3_3:-83473127 Msa0478630:CDS
GCAATGGAATGGATCAGATA+TGG 0.328027 3_3:-83472112 Msa0478630:CDS
ATAATGCAGGTTCTTACTAA+TGG 0.333844 3_3:-83472225 Msa0478630:intron
GCTGCTCCTTTGATGGGTTT+GGG 0.336272 3_3:+83471156 None:intergenic
TCACAATGCCACGAAGAAAA+AGG 0.336624 3_3:-83473713 Msa0478630:five_prime_UTR
CATATTTCTGATATAGTAGT+AGG 0.336697 3_3:-83470350 Msa0478630:three_prime_UTR
TCGGTTCTTGTTGGTCTTCA+AGG 0.350023 3_3:-83473154 Msa0478630:CDS
TCTACTAGCTGCTCCTTTGA+TGG 0.354669 3_3:+83471149 None:intergenic
CTTACGTGTCACTCATTCAT+TGG 0.366812 3_3:-83473574 Msa0478630:five_prime_UTR
TGGCTGCATTGTGGAGATAT+TGG 0.369691 3_3:-83471316 Msa0478630:CDS
GAGTGACTGGTAGTGTTTAT+TGG 0.371943 3_3:+83473517 None:intergenic
GAAAACACATATTTGTTTGC+TGG 0.379319 3_3:+83473773 None:intergenic
GAAGCAAATGGACGAGTTGC+AGG 0.380501 3_3:-83470549 Msa0478630:intron
GACGAGTTGCAGGTCATTAA+GGG 0.386548 3_3:-83470539 Msa0478630:CDS
GCGACTCATTTGCAAAGCTT+TGG 0.398902 3_3:-83473262 Msa0478630:CDS
GAAAAGATAGCGGAGAAAAT+GGG 0.401309 3_3:+83473414 None:intergenic
TTAATAGTTTAAGGTTATAG+AGG 0.402780 3_3:-83470327 Msa0478630:three_prime_UTR
ATTGCACGTTATCAAATTCA+AGG 0.405029 3_3:+83472130 None:intergenic
GTGAGAGGAGTGAGAGTGAC+TGG 0.413347 3_3:+83473504 None:intergenic
TCGATGTATTGCTGACCTGC+TGG 0.417680 3_3:-83470202 Msa0478630:three_prime_UTR
TGGAAATAACCGTACAAAAT+CGG 0.420784 3_3:-83473173 Msa0478630:CDS
TTGGATTAGGTGGTTGGCAT+TGG 0.430118 3_3:-83473114 Msa0478630:CDS
TACTATGTTACATATTAATC+GGG 0.431732 3_3:-83470379 Msa0478630:three_prime_UTR
TTCCCTTACGACCAGTTTCA+AGG 0.436157 3_3:+83470255 None:intergenic
CACAGAATGCATAGATCATT+GGG 0.437599 3_3:-83471561 Msa0478630:CDS
ATTAGGTGGTTGGCATTGGC+TGG 0.438971 3_3:-83473110 Msa0478630:intron
GCACAGAATGCATAGATCAT+TGG 0.439918 3_3:-83471562 Msa0478630:CDS
AATTACTAATATATACAATA+AGG 0.444214 3_3:+83470161 None:intergenic
CTACTAGCTGCTCCTTTGAT+GGG 0.444486 3_3:+83471150 None:intergenic
GGCGTTGTTAAGAAAGCTGT+TGG 0.447387 3_3:-83473133 Msa0478630:CDS
TGACTTGAGCAAGCTTGAAA+TGG 0.450063 3_3:-83471336 Msa0478630:CDS
CGTACAAAATCGGTTCTTGT+TGG 0.454461 3_3:-83473163 Msa0478630:CDS
TTGCAAAGCTTTGGTGGTGG+TGG 0.460216 3_3:-83473253 Msa0478630:CDS
GATATATGTGACCTTGAAAC+TGG 0.460934 3_3:-83470266 Msa0478630:three_prime_UTR
GGTAGTGTTTATTGGTGTGT+TGG 0.462216 3_3:+83473525 None:intergenic
AATGAGTGACACGTAAGATT+AGG 0.462750 3_3:+83473580 None:intergenic
TTGGTTCATTCCCTCTCGTC+GGG 0.468051 3_3:-83473555 Msa0478630:five_prime_UTR
GTTTGCTGGCAGAGTGAAGA+TGG 0.478149 3_3:+83473787 None:intergenic
GCCAATCCGCCACCACCCTA+TGG 0.479576 3_3:-83470079 Msa0478630:three_prime_UTR
ACTCATTTGCAAAGCTTTGG+TGG 0.481146 3_3:-83473259 Msa0478630:CDS
AGACTGTGTGCAAATGAGAT+TGG 0.492330 3_3:-83470490 Msa0478630:exon
TCTGGTGATTCACAGTCTAA+GGG 0.496708 3_3:-83471507 Msa0478630:CDS
AGTTCAGTTAACGGTGGTGG+TGG 0.498339 3_3:-83473289 Msa0478630:CDS
ATCATCTTCTTCATTGCCAG+TGG 0.499955 3_3:+83472155 None:intergenic
GGGTCCGTTACTATGCCTCA+TGG 0.500458 3_3:-83471486 Msa0478630:CDS
GCCATAGGGTGGTGGCGGAT+TGG 0.502890 3_3:+83470078 None:intergenic
AAGCTGTTGGATTAGGTGGT+TGG 0.508473 3_3:-83473120 Msa0478630:CDS
ATTGGTTCATTCCCTCTCGT+CGG 0.508541 3_3:-83473556 Msa0478630:five_prime_UTR
GCAATGGAAAGTTCAGTTAA+CGG 0.508721 3_3:-83473298 Msa0478630:CDS
ACCATGAGTAGGTCCTTCTC+TGG 0.512079 3_3:-83471525 Msa0478630:CDS
TGTGTTACCTCGTCATACTA+AGG 0.520326 3_3:-83473206 Msa0478630:CDS
TACTATGCCTCATGGGTCCA+TGG 0.522112 3_3:-83471478 Msa0478630:intron
TTGAATTTGATAACGTGCAA+TGG 0.522138 3_3:-83472128 Msa0478630:CDS
ATAACCACTTCATTAGCCAT+AGG 0.525655 3_3:+83470063 None:intergenic
TGACCTTGAAACTGGTCGTA+AGG 0.527053 3_3:-83470258 Msa0478630:three_prime_UTR
CATTTGCAAAGCTTTGGTGG+TGG 0.527791 3_3:-83473256 Msa0478630:CDS
AAAAGATAGCGGAGAAAATG+GGG 0.534955 3_3:+83473415 None:intergenic
AGACGAAATGATTGAAGCAA+TGG 0.538460 3_3:-83473314 Msa0478630:exon
GAGGCATTTCATGTCCCAGC+TGG 0.539302 3_3:-83471117 Msa0478630:intron
CAATGGAATGGATCAGATAT+GGG 0.541429 3_3:-83472111 Msa0478630:intron
CATTAGCCATAGGGTGGTGG+CGG 0.545825 3_3:+83470073 None:intergenic
CTAAAAGCTCACTTCCCAGC+AGG 0.547536 3_3:+83470187 None:intergenic
GAACATCGAAAAGAAAGCCT+TGG 0.547931 3_3:+83470228 None:intergenic
TGTGCGAATTTCATGAGTAA+AGG 0.552018 3_3:+83473390 None:intergenic
TAACCACTTCATTAGCCATA+GGG 0.553236 3_3:+83470064 None:intergenic
AAGAAAGCTGTTGGATTAGG+TGG 0.556533 3_3:-83473124 Msa0478630:CDS
ATTTGTCAATGCTGCAAAGA+GGG 0.558483 3_3:-83470516 Msa0478630:CDS
TTTGATAACGTGCAATGGAA+TGG 0.564949 3_3:-83472123 Msa0478630:CDS
CTTCATTAGCCATAGGGTGG+TGG 0.564958 3_3:+83470070 None:intergenic
TCATCTTCTTCATTGCCAGT+GGG 0.573087 3_3:+83472156 None:intergenic
AGCGTGATTGAAGCACCCAC+TGG 0.574928 3_3:-83472171 Msa0478630:CDS
GACCTTGAAACTGGTCGTAA+GGG 0.580014 3_3:-83470257 Msa0478630:three_prime_UTR
GGTCGTAAGGGAAGTCTCCA+AGG 0.583097 3_3:-83470245 Msa0478630:three_prime_UTR
CCACCCTATGGCTAATGAAG+TGG 0.584364 3_3:-83470067 Msa0478630:three_prime_UTR
GACACGTAAGATTAGGAAGG+TGG 0.584826 3_3:+83473587 None:intergenic
GAAAGTTCAGTTAACGGTGG+TGG 0.587165 3_3:-83473292 Msa0478630:CDS
AAGAAGAGAGAGAAAGTGAG+AGG 0.587528 3_3:+83473489 None:intergenic
CGATGTATTGCTGACCTGCT+GGG 0.594454 3_3:-83470201 Msa0478630:three_prime_UTR
TGTCGTCCCAAACCCATCAA+AGG 0.596940 3_3:-83471162 Msa0478630:CDS
GGTCCGTTACTATGCCTCAT+GGG 0.599829 3_3:-83471485 Msa0478630:CDS
CATATATCTACATAATAACT+GGG 0.605713 3_3:+83470281 None:intergenic
GAAGTGGTTATTAATCACCT+TGG 0.607458 3_3:-83470051 Msa0478630:three_prime_UTR
AGACTGTGAATCACCAGAGA+AGG 0.609518 3_3:+83471512 None:intergenic
ACCAGAGAAGGACCTACTCA+TGG 0.614361 3_3:+83471524 None:intergenic
GAATTGAAGTGAAGCTACAA+AGG 0.619531 3_3:-83472203 Msa0478630:CDS
CTGGTGATTCACAGTCTAAG+GGG 0.620120 3_3:-83471506 Msa0478630:CDS
AAACCAACCACGCAAACGCA+AGG 0.625045 3_3:-83473844 None:intergenic
AGCTAGTAGATGTTGTTCAG+AGG 0.626560 3_3:-83471136 Msa0478630:CDS
TGGTATGAATCCCGACGAGA+GGG 0.629840 3_3:+83473545 None:intergenic
TGTAACATAGTAAGTACGAA+GGG 0.633296 3_3:+83470390 None:intergenic
ATCATAGAACGGTCTTACCA+AGG 0.638709 3_3:+83470034 None:intergenic
ACACGTAAGATTAGGAAGGT+GGG 0.642218 3_3:+83473588 None:intergenic
TTGGTATGAATCCCGACGAG+AGG 0.642355 3_3:+83473544 None:intergenic
AGCTTGAAATGGCTGCATTG+TGG 0.645923 3_3:-83471325 Msa0478630:CDS
TGGTGGTGATAGTAGTGAAG+AGG 0.648847 3_3:-83473236 Msa0478630:CDS
CACACAAGTCTCACAAATCG+AGG 0.652726 3_3:-83471584 Msa0478630:CDS
GATTTGTCAATGCTGCAAAG+AGG 0.660589 3_3:-83470517 Msa0478630:CDS
AGTGACACGTAAGATTAGGA+AGG 0.661679 3_3:+83473584 None:intergenic
AAAGATAGCGGAGAAAATGG+GGG 0.664645 3_3:+83473416 None:intergenic
GAGTAAAGGTGAAAAGATAG+CGG 0.665485 3_3:+83473404 None:intergenic
ATGTAACATAGTAAGTACGA+AGG 0.675651 3_3:+83470389 None:intergenic
GGACCCATGAGGCATAGTAA+CGG 0.688810 3_3:+83471482 None:intergenic
CCACTTCATTAGCCATAGGG+TGG 0.692185 3_3:+83470067 None:intergenic
AACATAGTAAGTACGAAGGG+AGG 0.698574 3_3:+83470393 None:intergenic
CATGTCACAAGACCATGAGT+AGG 0.700516 3_3:-83471536 Msa0478630:CDS
TGATATACCATGGACCCATG+AGG 0.707981 3_3:+83471471 None:intergenic
ATATCTACATAATAACTGGG+TGG 0.711276 3_3:+83470284 None:intergenic
ACAACAACCTTAGTATGACG+AGG 0.714849 3_3:+83473199 None:intergenic
ATGGAAAGTTCAGTTAACGG+TGG 0.729999 3_3:-83473295 Msa0478630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTCTATTTTTTATTATTA+AGG - chr3_3:83470756-83470775 Msa0478630:intron 10.0%
!!! ATTGTTTATTAAGTTTATTT+TGG - chr3_3:83471223-83471242 Msa0478630:intron 10.0%
!!! TTGTTTATTAAGTTTATTTT+GGG - chr3_3:83471224-83471243 Msa0478630:intron 10.0%
!! TAACACAATTTATTTAATAT+TGG + chr3_3:83471308-83471327 None:intergenic 10.0%
!! TATTAAATAAATTGTGTTAT+TGG - chr3_3:83471309-83471328 Msa0478630:CDS 10.0%
!!! AATTTAGATTATTTGAAATT+AGG - chr3_3:83471535-83471554 Msa0478630:CDS 10.0%
!! TTGTTAAAAAAAAATCATTT+AGG + chr3_3:83471561-83471580 None:intergenic 10.0%
!! ATAATTATTAAAAATAGTAC+CGG + chr3_3:83472171-83472190 None:intergenic 10.0%
!! AATTACTAATATATACAATA+AGG + chr3_3:83473662-83473681 None:intergenic 10.0%
!!! AATTTTTTTTTGTCCTTTTT+TGG - chr3_3:83470083-83470102 Msa0478630:three_prime_UTR 15.0%
!!! TGTTTATTAAGTTTATTTTG+GGG - chr3_3:83471225-83471244 Msa0478630:intron 15.0%
!!! ATATGCATTTTTATTTTTCA+TGG - chr3_3:83471382-83471401 Msa0478630:intron 15.0%
!!! ATTGTATTGTTTTTTCTTTA+GGG + chr3_3:83471879-83471898 None:intergenic 15.0%
!!! AATTGTATTGTTTTTTCTTT+AGG + chr3_3:83471880-83471899 None:intergenic 15.0%
!!! TAGTTTTTTAATTGTTGTTT+TGG - chr3_3:83472005-83472024 Msa0478630:intron 15.0%
!! AAGATAGAAACTTAATAATA+TGG + chr3_3:83472840-83472859 None:intergenic 15.0%
!! TTACTATGTTACATATTAAT+CGG - chr3_3:83473440-83473459 Msa0478630:five_prime_UTR 15.0%
!!! CATTTCATTTCTAATTTTCT+GGG + chr3_3:83470155-83470174 None:intergenic 20.0%
!!! TCATTTCATTTCTAATTTTC+TGG + chr3_3:83470156-83470175 None:intergenic 20.0%
!! TGAAATTGAAATGATAAGAA+TGG + chr3_3:83470804-83470823 None:intergenic 20.0%
!!! TGATAACCGTTTTTTTTTTT+CGG - chr3_3:83470940-83470959 Msa0478630:intron 20.0%
!! TAAAACCCGAAAAAAAAAAA+CGG + chr3_3:83470949-83470968 None:intergenic 20.0%
!!! AATTTTGATTCTCAAAATGT+TGG - chr3_3:83471041-83471060 Msa0478630:intron 20.0%
!!! ATTTTGATTCTCAAAATGTT+GGG - chr3_3:83471042-83471061 Msa0478630:intron 20.0%
!! ATATTGGAAAATAATACCAA+AGG + chr3_3:83471292-83471311 None:intergenic 20.0%
!! ACTTTATTAATAATTCTGAC+TGG - chr3_3:83471760-83471779 Msa0478630:intron 20.0%
!! GTTAATCATTATTATTGAGA+TGG + chr3_3:83471839-83471858 None:intergenic 20.0%
!!! TTGTATTGTTTTTTCTTTAG+GGG + chr3_3:83471878-83471897 None:intergenic 20.0%
!! TGGTATATAGAGTAAAAAAA+AGG + chr3_3:83472101-83472120 None:intergenic 20.0%
!!! TCCTATAATAACTAATCTTT+TGG - chr3_3:83472548-83472567 Msa0478630:intron 20.0%
!!! ACCAAAAGATTAGTTATTAT+AGG + chr3_3:83472552-83472571 None:intergenic 20.0%
!!! CTTAGTACTTACTTTTAAAA+TGG - chr3_3:83472622-83472641 Msa0478630:intron 20.0%
!! ATAGTGCAAATATTCTAAAT+AGG + chr3_3:83472917-83472936 None:intergenic 20.0%
!!! TTAAGTTTAAAATTGTGCAT+TGG - chr3_3:83473079-83473098 Msa0478630:intron 20.0%
!! TACTATGTTACATATTAATC+GGG - chr3_3:83473441-83473460 Msa0478630:five_prime_UTR 20.0%
!!! TTAATAGTTTAAGGTTATAG+AGG - chr3_3:83473493-83473512 Msa0478630:five_prime_UTR 20.0%
!! CATATATCTACATAATAACT+GGG + chr3_3:83473542-83473561 None:intergenic 20.0%
!! ACATATATCTACATAATAAC+TGG + chr3_3:83473543-83473562 None:intergenic 20.0%
!! AAAAACTTCAAATCATAGAA+CGG + chr3_3:83473800-83473819 None:intergenic 20.0%
!!! TTGTTGTTATCAAAAGTATT+AGG - chr3_3:83473826-83473845 Msa0478630:five_prime_UTR 20.0%
!!! TTTTTTTTCCTTTTTCTTCG+TGG + chr3_3:83470118-83470137 None:intergenic 25.0%
!!! GATAACCGTTTTTTTTTTTC+GGG - chr3_3:83470941-83470960 Msa0478630:intron 25.0%
! GGATAAGAATTCATAAAGTA+GGG + chr3_3:83470993-83471012 None:intergenic 25.0%
! TGGATAAGAATTCATAAAGT+AGG + chr3_3:83470994-83471013 None:intergenic 25.0%
! AATTCTTATCCAAATCATTC+AGG - chr3_3:83471002-83471021 Msa0478630:intron 25.0%
! AAATCAAAACCTGAATGATT+TGG + chr3_3:83471014-83471033 None:intergenic 25.0%
!!! TTTTGATTCTCAAAATGTTG+GGG - chr3_3:83471043-83471062 Msa0478630:intron 25.0%
!!! ATTTTTTGCTGTTGTTTATG+TGG - chr3_3:83471093-83471112 Msa0478630:intron 25.0%
!!! TTTTTTGCTGTTGTTTATGT+GGG - chr3_3:83471094-83471113 Msa0478630:intron 25.0%
!! ATTGGATTTTCTGAATCTAA+TGG - chr3_3:83471327-83471346 Msa0478630:CDS 25.0%
!! TTGGATTTTCTGAATCTAAT+GGG - chr3_3:83471328-83471347 Msa0478630:CDS 25.0%
!! AATCTAATGGGGATTTTATT+TGG - chr3_3:83471340-83471359 Msa0478630:CDS 25.0%
!!! ATCTAATGGGGATTTTATTT+GGG - chr3_3:83471341-83471360 Msa0478630:CDS 25.0%
! TGCAATTAACTAATTATCAG+TGG - chr3_3:83471441-83471460 Msa0478630:intron 25.0%
! ATTGAAATCAAATTTGTCGT+AGG - chr3_3:83471465-83471484 Msa0478630:intron 25.0%
! TTTAGCTGTATAAATAATGC+AGG - chr3_3:83471582-83471601 Msa0478630:CDS 25.0%
!! CTGTTCTGTTTTATCTTAAA+GGG - chr3_3:83471788-83471807 Msa0478630:intron 25.0%
! TCATTATTATTGAGATGGAA+TGG + chr3_3:83471834-83471853 None:intergenic 25.0%
! AGTTGAATCACATTAATTCT+AGG + chr3_3:83471924-83471943 None:intergenic 25.0%
! TATTTATTTATTGACCCTAC+CGG - chr3_3:83472149-83472168 Msa0478630:CDS 25.0%
!!! TATTAAAAATAGTACCGGTA+GGG + chr3_3:83472166-83472185 None:intergenic 25.0%
!!! TTATTAAAAATAGTACCGGT+AGG + chr3_3:83472167-83472186 None:intergenic 25.0%
! TATCTTATTGAAGATGCTTA+TGG - chr3_3:83472577-83472596 Msa0478630:intron 25.0%
!!! TACTTTTAAAATGGATTTGC+AGG - chr3_3:83472631-83472650 Msa0478630:intron 25.0%
!! ATAATTTTTATGTACCAGCT+GGG + chr3_3:83472720-83472739 None:intergenic 25.0%
!! AATAATTTTTATGTACCAGC+TGG + chr3_3:83472721-83472740 None:intergenic 25.0%
!! ACAAGAGTTCTTTATTTCAA+TGG + chr3_3:83472774-83472793 None:intergenic 25.0%
! AATTTGAACTGTTGTAATTG+AGG - chr3_3:83472796-83472815 Msa0478630:intron 25.0%
! CTAAATAGGTCAAGTAATAT+GGG + chr3_3:83472903-83472922 None:intergenic 25.0%
! TCTAAATAGGTCAAGTAATA+TGG + chr3_3:83472904-83472923 None:intergenic 25.0%
!! AAGTTCTACAACAAACTTTT+AGG + chr3_3:83473189-83473208 None:intergenic 25.0%
!! CATATTTCTGATATAGTAGT+AGG - chr3_3:83473470-83473489 Msa0478630:five_prime_UTR 25.0%
! AGTAGTAGGTTAATAGTTTA+AGG - chr3_3:83473484-83473503 Msa0478630:five_prime_UTR 25.0%
GAAAACACATATTTGTTTGC+TGG + chr3_3:83470050-83470069 None:intergenic 30.0%
TGGAAATAACCGTACAAAAT+CGG - chr3_3:83470647-83470666 Msa0478630:intron 30.0%
! TTCAAACCATTTTCTTGTGT+CGG - chr3_3:83470820-83470839 Msa0478630:intron 30.0%
TGCATGTTTCTCTTTATTAC+TGG - chr3_3:83471158-83471177 Msa0478630:CDS 30.0%
TTTCTCTTTATTACTGGATC+TGG - chr3_3:83471164-83471183 Msa0478630:CDS 30.0%
AAAATAATACCAAAGGAGGT+AGG + chr3_3:83471285-83471304 None:intergenic 30.0%
TTGGAAAATAATACCAAAGG+AGG + chr3_3:83471289-83471308 None:intergenic 30.0%
! TGGATTTTCTGAATCTAATG+GGG - chr3_3:83471329-83471348 Msa0478630:CDS 30.0%
ATAATGCAGGTTCTTACTAA+TGG - chr3_3:83471595-83471614 Msa0478630:CDS 30.0%
ATTGCACGTTATCAAATTCA+AGG + chr3_3:83471693-83471712 None:intergenic 30.0%
!! TTGAATTTGATAACGTGCAA+TGG - chr3_3:83471692-83471711 Msa0478630:intron 30.0%
! GCTGTTCTGTTTTATCTTAA+AGG - chr3_3:83471787-83471806 Msa0478630:intron 30.0%
! GATTTAAAGCTGATATACCA+TGG + chr3_3:83472362-83472381 None:intergenic 30.0%
AAAATTATCTAATGCGTCAG+TGG + chr3_3:83472407-83472426 None:intergenic 30.0%
! CTTTGTATATTTTCTGCAGA+AGG - chr3_3:83472460-83472479 Msa0478630:intron 30.0%
!!! TTTTAAAATGGATTTGCAGG+TGG - chr3_3:83472634-83472653 Msa0478630:intron 30.0%
GAGGGAGTAATTTACAAATT+CGG + chr3_3:83472877-83472896 None:intergenic 30.0%
TCAACTAGGTCAAGTAATAT+GGG - chr3_3:83473116-83473135 Msa0478630:CDS 30.0%
CAAGAAAGAAACACAATTTC+AGG + chr3_3:83473250-83473269 None:intergenic 30.0%
TTTCTTTCTTGTGAAGCAAA+TGG - chr3_3:83473259-83473278 Msa0478630:CDS 30.0%
TGTAACATAGTAAGTACGAA+GGG + chr3_3:83473433-83473452 None:intergenic 30.0%
ATGTAACATAGTAAGTACGA+AGG + chr3_3:83473434-83473453 None:intergenic 30.0%
! AGGTACTAATTACTCTTTTG+TGG - chr3_3:83473513-83473532 Msa0478630:five_prime_UTR 30.0%
ATATCTACATAATAACTGGG+TGG + chr3_3:83473539-83473558 None:intergenic 30.0%
AATGAGTGACACGTAAGATT+AGG + chr3_3:83470243-83470262 None:intergenic 35.0%
!! AAAAGATAGCGGAGAAAATG+GGG + chr3_3:83470408-83470427 None:intergenic 35.0%
!! GAAAAGATAGCGGAGAAAAT+GGG + chr3_3:83470409-83470428 None:intergenic 35.0%
!! TGAAAAGATAGCGGAGAAAA+TGG + chr3_3:83470410-83470429 None:intergenic 35.0%
GAGTAAAGGTGAAAAGATAG+CGG + chr3_3:83470419-83470438 None:intergenic 35.0%
TGTGCGAATTTCATGAGTAA+AGG + chr3_3:83470433-83470452 None:intergenic 35.0%
AGACGAAATGATTGAAGCAA+TGG - chr3_3:83470506-83470525 Msa0478630:CDS 35.0%
GCAATGGAAAGTTCAGTTAA+CGG - chr3_3:83470522-83470541 Msa0478630:CDS 35.0%
! CATACTAAGGTTGTTGTTAC+TGG - chr3_3:83470627-83470646 Msa0478630:intron 35.0%
!! CAACAAGAACCGATTTTGTA+CGG + chr3_3:83470659-83470678 None:intergenic 35.0%
GTTAAGAAAGCTGTTGGATT+AGG - chr3_3:83470693-83470712 Msa0478630:intron 35.0%
AATTGACCGACACAAGAAAA+TGG + chr3_3:83470829-83470848 None:intergenic 35.0%
!! TTTCTTGTGTCGGTCAATTT+TGG - chr3_3:83470830-83470849 Msa0478630:intron 35.0%
TTAGATTTCAGCGTGATTGA+AGG + chr3_3:83470902-83470921 None:intergenic 35.0%
GAGAAACATGCAATGAATAC+AGG + chr3_3:83471150-83471169 None:intergenic 35.0%
AGTATATTACCTACCTCCTT+TGG - chr3_3:83471273-83471292 Msa0478630:intron 35.0%
GAATTGAAGTGAAGCTACAA+AGG - chr3_3:83471617-83471636 Msa0478630:intron 35.0%
!! TTTGATAACGTGCAATGGAA+TGG - chr3_3:83471697-83471716 Msa0478630:intron 35.0%
CAATGGAATGGATCAGATAT+GGG - chr3_3:83471709-83471728 Msa0478630:intron 35.0%
CAGAAACAAGTACTAGTACT+CGG + chr3_3:83471961-83471980 None:intergenic 35.0%
GTTTGCAAAGTGATAGAGTT+TGG - chr3_3:83472043-83472062 Msa0478630:intron 35.0%
! GTGATAGAGTTTGGTAACAT+TGG - chr3_3:83472052-83472071 Msa0478630:intron 35.0%
GGAAAAAAAGACACGCATAA+AGG + chr3_3:83472080-83472099 None:intergenic 35.0%
! GCAAAACACTAGAGTCTTTT+TGG + chr3_3:83472121-83472140 None:intergenic 35.0%
CACAGAATGCATAGATCATT+GGG - chr3_3:83472259-83472278 Msa0478630:intron 35.0%
TACAAATTCGGCGTAATTTG+TGG + chr3_3:83472865-83472884 None:intergenic 35.0%
ATAGGTCAAGTAATATGGGA+CGG + chr3_3:83472899-83472918 None:intergenic 35.0%
GTCAACTAGGTCAAGTAATA+TGG - chr3_3:83473115-83473134 Msa0478630:CDS 35.0%
CTGATATGCATGAATTGTGA+TGG - chr3_3:83473152-83473171 Msa0478630:CDS 35.0%
ATTTGTCAATGCTGCAAAGA+GGG - chr3_3:83473304-83473323 Msa0478630:CDS 35.0%
GATATATGTGACCTTGAAAC+TGG - chr3_3:83473554-83473573 Msa0478630:five_prime_UTR 35.0%
TAACCACTTCATTAGCCATA+GGG + chr3_3:83473759-83473778 None:intergenic 35.0%
ATAACCACTTCATTAGCCAT+AGG + chr3_3:83473760-83473779 None:intergenic 35.0%
GAAGTGGTTATTAATCACCT+TGG - chr3_3:83473769-83473788 Msa0478630:five_prime_UTR 35.0%
GTGGCATTGTGATCCAAAAA+AGG + chr3_3:83470099-83470118 None:intergenic 40.0%
TCACAATGCCACGAAGAAAA+AGG - chr3_3:83470107-83470126 Msa0478630:three_prime_UTR 40.0%
ACACGTAAGATTAGGAAGGT+GGG + chr3_3:83470235-83470254 None:intergenic 40.0%
AGTGACACGTAAGATTAGGA+AGG + chr3_3:83470239-83470258 None:intergenic 40.0%
! CTTACGTGTCACTCATTCAT+TGG - chr3_3:83470246-83470265 Msa0478630:three_prime_UTR 40.0%
GGTAGTGTTTATTGGTGTGT+TGG + chr3_3:83470298-83470317 None:intergenic 40.0%
! GAGTGACTGGTAGTGTTTAT+TGG + chr3_3:83470306-83470325 None:intergenic 40.0%
AAGAAGAGAGAGAAAGTGAG+AGG + chr3_3:83470334-83470353 None:intergenic 40.0%
!! AAAGATAGCGGAGAAAATGG+GGG + chr3_3:83470407-83470426 None:intergenic 40.0%
ATGGAAAGTTCAGTTAACGG+TGG - chr3_3:83470525-83470544 Msa0478630:CDS 40.0%
! ACTCATTTGCAAAGCTTTGG+TGG - chr3_3:83470561-83470580 Msa0478630:intron 40.0%
TGTGTTACCTCGTCATACTA+AGG - chr3_3:83470614-83470633 Msa0478630:intron 40.0%
ACAACAACCTTAGTATGACG+AGG + chr3_3:83470624-83470643 None:intergenic 40.0%
! CGTACAAAATCGGTTCTTGT+TGG - chr3_3:83470657-83470676 Msa0478630:intron 40.0%
! AAGAAAGCTGTTGGATTAGG+TGG - chr3_3:83470696-83470715 Msa0478630:intron 40.0%
TCATCTTCTTCATTGCCAGT+GGG + chr3_3:83471667-83471686 None:intergenic 40.0%
ATCATCTTCTTCATTGCCAG+TGG + chr3_3:83471668-83471687 None:intergenic 40.0%
GCAATGGAATGGATCAGATA+TGG - chr3_3:83471708-83471727 Msa0478630:intron 40.0%
TCTATCACTTTGCAAACCCA+TGG + chr3_3:83472040-83472059 None:intergenic 40.0%
GCACAGAATGCATAGATCAT+TGG - chr3_3:83472258-83472277 Msa0478630:intron 40.0%
TCTGGTGATTCACAGTCTAA+GGG - chr3_3:83472313-83472332 Msa0478630:intron 40.0%
TATCTAATGCGTCAGTGGTA+GGG + chr3_3:83472402-83472421 None:intergenic 40.0%
TTATCTAATGCGTCAGTGGT+AGG + chr3_3:83472403-83472422 None:intergenic 40.0%
TGACTTGAGCAAGCTTGAAA+TGG - chr3_3:83472484-83472503 Msa0478630:intron 40.0%
AGCTAGTAGATGTTGTTCAG+AGG - chr3_3:83472684-83472703 Msa0478630:intron 40.0%
CTAGGTCAAGTAATATGGGA+CGG - chr3_3:83473120-83473139 Msa0478630:CDS 40.0%
GATTTGTCAATGCTGCAAAG+AGG - chr3_3:83473303-83473322 Msa0478630:CDS 40.0%
AGACTGTGTGCAAATGAGAT+TGG - chr3_3:83473330-83473349 Msa0478630:five_prime_UTR 40.0%
AACATAGTAAGTACGAAGGG+AGG + chr3_3:83473430-83473449 None:intergenic 40.0%
GAACATCGAAAAGAAAGCCT+TGG + chr3_3:83473595-83473614 None:intergenic 40.0%
ATCATAGAACGGTCTTACCA+AGG + chr3_3:83473789-83473808 None:intergenic 40.0%
GACACGTAAGATTAGGAAGG+TGG + chr3_3:83470236-83470255 None:intergenic 45.0%
! ATTGGTTCATTCCCTCTCGT+CGG - chr3_3:83470264-83470283 Msa0478630:three_prime_UTR 45.0%
GGAGAAAATGGGGGAAATGT+AGG + chr3_3:83470398-83470417 None:intergenic 45.0%
GAAAGTTCAGTTAACGGTGG+TGG - chr3_3:83470528-83470547 Msa0478630:CDS 45.0%
GCGACTCATTTGCAAAGCTT+TGG - chr3_3:83470558-83470577 Msa0478630:intron 45.0%
! CATTTGCAAAGCTTTGGTGG+TGG - chr3_3:83470564-83470583 Msa0478630:intron 45.0%
! TGGTGGTGATAGTAGTGAAG+AGG - chr3_3:83470584-83470603 Msa0478630:intron 45.0%
!! TCGGTTCTTGTTGGTCTTCA+AGG - chr3_3:83470666-83470685 Msa0478630:intron 45.0%
GGCGTTGTTAAGAAAGCTGT+TGG - chr3_3:83470687-83470706 Msa0478630:intron 45.0%
! AAGCTGTTGGATTAGGTGGT+TGG - chr3_3:83470700-83470719 Msa0478630:intron 45.0%
! TTGGATTAGGTGGTTGGCAT+TGG - chr3_3:83470706-83470725 Msa0478630:intron 45.0%
!!! TGTTTTGGCTTCCATGACCA+TGG - chr3_3:83472020-83472039 Msa0478630:intron 45.0%
!!! GTTTTGGCTTCCATGACCAT+GGG - chr3_3:83472021-83472040 Msa0478630:intron 45.0%
ACTTTGCAAACCCATGGTCA+TGG + chr3_3:83472034-83472053 None:intergenic 45.0%
CACACAAGTCTCACAAATCG+AGG - chr3_3:83472236-83472255 Msa0478630:intron 45.0%
CATGTCACAAGACCATGAGT+AGG - chr3_3:83472284-83472303 Msa0478630:intron 45.0%
AGACTGTGAATCACCAGAGA+AGG + chr3_3:83472311-83472330 None:intergenic 45.0%
CTCTGGTGATTCACAGTCTA+AGG - chr3_3:83472312-83472331 Msa0478630:intron 45.0%
CTGGTGATTCACAGTCTAAG+GGG - chr3_3:83472314-83472333 Msa0478630:intron 45.0%
TGATATACCATGGACCCATG+AGG + chr3_3:83472352-83472371 None:intergenic 45.0%
AGCTTGAAATGGCTGCATTG+TGG - chr3_3:83472495-83472514 Msa0478630:intron 45.0%
TGGCTGCATTGTGGAGATAT+TGG - chr3_3:83472504-83472523 Msa0478630:intron 45.0%
CTACTAGCTGCTCCTTTGAT+GGG + chr3_3:83472673-83472692 None:intergenic 45.0%
TCTACTAGCTGCTCCTTTGA+TGG + chr3_3:83472674-83472693 None:intergenic 45.0%
GTCAAGTAATATGGGACGGA+GGG + chr3_3:83473124-83473143 None:intergenic 45.0%
CATACGTGACAGAGTCAACT+AGG - chr3_3:83473102-83473121 Msa0478630:intron 45.0%
GTCAAGTAATATGGGACGGA+GGG - chr3_3:83473124-83473143 Msa0478630:CDS 45.0%
GACGAGTTGCAGGTCATTAA+GGG - chr3_3:83473281-83473300 Msa0478630:CDS 45.0%
TGACCTTGAAACTGGTCGTA+AGG - chr3_3:83473562-83473581 Msa0478630:five_prime_UTR 45.0%
GACCTTGAAACTGGTCGTAA+GGG - chr3_3:83473563-83473582 Msa0478630:five_prime_UTR 45.0%
TTCCCTTACGACCAGTTTCA+AGG + chr3_3:83473568-83473587 None:intergenic 45.0%
!! CATTAATAATTAAAATATTA+AGG + chr3_3:83472433-83472452 None:intergenic 5.0%
! GTTTGCTGGCAGAGTGAAGA+TGG + chr3_3:83470036-83470055 None:intergenic 50.0%
GAGAGACTAGAGAGAGAGAG+CGG + chr3_3:83470183-83470202 None:intergenic 50.0%
TTGGTTCATTCCCTCTCGTC+GGG - chr3_3:83470265-83470284 Msa0478630:three_prime_UTR 50.0%
TGGTATGAATCCCGACGAGA+GGG + chr3_3:83470278-83470297 None:intergenic 50.0%
TTGGTATGAATCCCGACGAG+AGG + chr3_3:83470279-83470298 None:intergenic 50.0%
! AGTTCAGTTAACGGTGGTGG+TGG - chr3_3:83470531-83470550 Msa0478630:CDS 50.0%
!! TTGCAAAGCTTTGGTGGTGG+TGG - chr3_3:83470567-83470586 Msa0478630:intron 50.0%
!! ATTAGGTGGTTGGCATTGGC+TGG - chr3_3:83470710-83470729 Msa0478630:intron 50.0%
ACCATGAGTAGGTCCTTCTC+TGG - chr3_3:83472295-83472314 Msa0478630:intron 50.0%
ACCAGAGAAGGACCTACTCA+TGG + chr3_3:83472299-83472318 None:intergenic 50.0%
GGTCCGTTACTATGCCTCAT+GGG - chr3_3:83472335-83472354 Msa0478630:intron 50.0%
GGACCCATGAGGCATAGTAA+CGG + chr3_3:83472341-83472360 None:intergenic 50.0%
TACTATGCCTCATGGGTCCA+TGG - chr3_3:83472342-83472361 Msa0478630:intron 50.0%
TGTCGTCCCAAACCCATCAA+AGG - chr3_3:83472658-83472677 Msa0478630:intron 50.0%
!! GCTGCTCCTTTGATGGGTTT+GGG + chr3_3:83472667-83472686 None:intergenic 50.0%
!! AGCTGCTCCTTTGATGGGTT+TGG + chr3_3:83472668-83472687 None:intergenic 50.0%
GGTCAAGTAATATGGGACGG+AGG + chr3_3:83473123-83473142 None:intergenic 50.0%
GGTCAAGTAATATGGGACGG+AGG - chr3_3:83473123-83473142 Msa0478630:CDS 50.0%
GAAGCAAATGGACGAGTTGC+AGG - chr3_3:83473271-83473290 Msa0478630:CDS 50.0%
GGACGAGTTGCAGGTCATTA+AGG - chr3_3:83473280-83473299 Msa0478630:CDS 50.0%
TCGATGTATTGCTGACCTGC+TGG - chr3_3:83473618-83473637 Msa0478630:five_prime_UTR 50.0%
CGATGTATTGCTGACCTGCT+GGG - chr3_3:83473619-83473638 Msa0478630:five_prime_UTR 50.0%
CTAAAAGCTCACTTCCCAGC+AGG + chr3_3:83473636-83473655 None:intergenic 50.0%
CTTCATTAGCCATAGGGTGG+TGG + chr3_3:83473753-83473772 None:intergenic 50.0%
CCACTTCATTAGCCATAGGG+TGG + chr3_3:83473756-83473775 None:intergenic 50.0%
CCACCCTATGGCTAATGAAG+TGG - chr3_3:83473753-83473772 Msa0478630:five_prime_UTR 50.0%
GTGAGAGGAGTGAGAGTGAC+TGG + chr3_3:83470319-83470338 None:intergenic 55.0%
! AGCGTGATTGAAGCACCCAC+TGG - chr3_3:83471649-83471668 Msa0478630:intron 55.0%
GGGTCCGTTACTATGCCTCA+TGG - chr3_3:83472334-83472353 Msa0478630:intron 55.0%
! GAGGCATTTCATGTCCCAGC+TGG - chr3_3:83472703-83472722 Msa0478630:intron 55.0%
GGTCGTAAGGGAAGTCTCCA+AGG - chr3_3:83473575-83473594 Msa0478630:five_prime_UTR 55.0%
! CATTAGCCATAGGGTGGTGG+CGG + chr3_3:83473750-83473769 None:intergenic 55.0%
GCCAATCCGCCACCACCCTA+TGG - chr3_3:83473741-83473760 Msa0478630:five_prime_UTR 65.0%
!! GCCATAGGGTGGTGGCGGAT+TGG + chr3_3:83473745-83473764 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 83469987 83473855 83469987 ID=Msa0478630;Name=Msa0478630
chr3_3 mRNA 83469987 83473855 83469987 ID=Msa0478630-mRNA-1;Parent=Msa0478630;Name=Msa0478630-mRNA-1;_AED=0.12;_eAED=0.12;_QI=526|1|1|1|1|1|6|509|230
chr3_3 exon 83469987 83470567 83469987 ID=Msa0478630-mRNA-1:exon:23443;Parent=Msa0478630-mRNA-1
chr3_3 exon 83471118 83471189 83471118 ID=Msa0478630-mRNA-1:exon:23442;Parent=Msa0478630-mRNA-1
chr3_3 exon 83471301 83471363 83471301 ID=Msa0478630-mRNA-1:exon:23441;Parent=Msa0478630-mRNA-1
chr3_3 exon 83471479 83471618 83471479 ID=Msa0478630-mRNA-1:exon:23440;Parent=Msa0478630-mRNA-1
chr3_3 exon 83472112 83472238 83472112 ID=Msa0478630-mRNA-1:exon:23439;Parent=Msa0478630-mRNA-1
chr3_3 exon 83473111 83473855 83473111 ID=Msa0478630-mRNA-1:exon:23438;Parent=Msa0478630-mRNA-1
chr3_3 five_prime_UTR 83473330 83473855 83473330 ID=Msa0478630-mRNA-1:five_prime_utr;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83473111 83473329 83473111 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83472112 83472238 83472112 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83471479 83471618 83471479 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83471301 83471363 83471301 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83471118 83471189 83471118 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 CDS 83470496 83470567 83470496 ID=Msa0478630-mRNA-1:cds;Parent=Msa0478630-mRNA-1
chr3_3 three_prime_UTR 83469987 83470495 83469987 ID=Msa0478630-mRNA-1:three_prime_utr;Parent=Msa0478630-mRNA-1
Gene Sequence

>Msa0478630

ATGATTGAAGCAATGGAAAGTTCAGTTAACGGTGGTGGTGGTTTCGCGACTCATTTGCAAAGCTTTGGTGGTGGTGGTGATAGTAGTGAAGAGGAACTGTCTGTGTTACCTCGTCATACTAAGGTTGTTGTTACTGGAAATAACCGTACAAAATCGGTTCTTGTTGGTCTTCAAGGCGTTGTTAAGAAAGCTGTTGGATTAGGTGGTTGGCATTGGCTGGTTCTTACTAATGGAATTGAAGTGAAGCTACAAAGGAATGCTCTTAGCGTGATTGAAGCACCCACTGGCAATGAAGAAGATGATGACCTTGAATTTGATAACGTGCAATGGAATGGATCAGATATGGCATCAGACGATACACACAAGTCTCACAAATCGAGGCACAGAATGCATAGATCATTGGGATCATGTCACAAGACCATGAGTAGGTCCTTCTCTGGTGATTCACAGTCTAAGGGGTCCGTTACTATGCCTCATGGGTCCATGAAGGTTGACTTGAGCAAGCTTGAAATGGCTGCATTGTGGAGATATTGGAGACATTTTAATCTTGTGGATGTCGTCCCAAACCCATCAAAGGAGCAGCTAGTAGATGTTGTTCAGAGGCATTTCATGTCCCAGCTGCAAATGGACGAGTTGCAGGTCATTAAGGGATTTGTCAATGCTGCAAAGAGGGTCAAGACTGTGTGCAAATGA
Protein sequence

>Msa0478630

MIEAMESSVNGGGGFATHLQSFGGGGDSSEEELSVLPRHTKVVVTGNNRTKSVLVGLQGVVKKAVGLGGWHWLVLTNGIEVKLQRNALSVIEAPTGNEEDDDLEFDNVQWNGSDMASDDTHKSHKSRHRMHRSLGSCHKTMSRSFSGDSQSKGSVTMPHGSMKVDLSKLEMAALWRYWRHFNLVDVVPNPSKEQLVDVVQRHFMSQLQMDELQVIKGFVNAAKRVKTVCK*