AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0430370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430370 MtrunA17_Chr3g0141391 98.701 231 2 1 1 230 1 231 2.14e-169 465
Msa0430370 MtrunA17_Chr1g0154621 83.550 231 32 4 1 230 1 226 1.33e-136 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0430370 AT1G19330.2 76.522 230 51 3 1 230 1 227 6.26e-111 318
Msa0430370 AT1G19330.1 75.319 235 50 4 1 230 1 232 3.24e-109 314
Msa0430370 AT1G19330.3 75.000 236 50 5 1 230 1 233 5.65e-108 311
Msa0430370 AT1G75060.1 69.787 235 64 5 1 230 1 233 2.50e-106 306
Msa0430370 AT1G75060.2 69.362 235 64 6 1 230 1 232 8.14e-104 300
Msa0430370 AT1G75060.3 70.370 189 50 4 1 184 1 188 1.03e-80 240

Find 119 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGTAGGTTAATAGTTTA+AGG 0.109374 3_2:-82176174 Msa0430370:three_prime_UTR
CATACTAAGGTTGTTGTTAC+TGG 0.194013 3_2:-82178730 Msa0430370:CDS
AGCTGCTCCTTTGATGGGTT+TGG 0.231841 3_2:+82176732 None:intergenic
CTCTGGTGATTCACAGTCTA+AGG 0.252293 3_2:-82177085 Msa0430370:CDS
GGACGAGTTGCAGGTCATTA+AGG 0.256826 3_2:-82176378 Msa0430370:CDS
TTACTATGTTACATATTAAT+CGG 0.270714 3_2:-82176218 Msa0430370:three_prime_UTR
ACATATATCTACATAATAAC+TGG 0.282748 3_2:+82176118 None:intergenic
TGAAAAGATAGCGGAGAAAA+TGG 0.292286 3_2:+82178950 None:intergenic
TTGTTGTTATCAAAAGTATT+AGG 0.312640 3_2:-82175832 Msa0430370:three_prime_UTR
TCTGTTCTTGTTGGTCTTCA+AGG 0.325218 3_2:-82178691 Msa0430370:CDS
GTTAAGAAAGCTGTTGGATT+AGG 0.325633 3_2:-82178664 Msa0430370:CDS
GCAATGGAATGGATCAGATA+TGG 0.328027 3_2:-82177661 Msa0430370:CDS
ATAATGCAGGTTCTTACTAA+TGG 0.333844 3_2:-82177774 Msa0430370:intron
GCTGCTCCTTTGATGGGTTT+GGG 0.336272 3_2:+82176733 None:intergenic
TCACAATGCCACGAAGAAAA+AGG 0.336624 3_2:-82179248 Msa0430370:five_prime_UTR
CATATTTCTGATATAGTAGT+AGG 0.336697 3_2:-82176188 Msa0430370:three_prime_UTR
TCAACTAGCTGCTCCTTTGA+TGG 0.362105 3_2:+82176726 None:intergenic
CTTACGTGTCACTCATTCAT+TGG 0.366812 3_2:-82179111 Msa0430370:five_prime_UTR
TGGCTGCATTGTGGAGATAT+TGG 0.369691 3_2:-82176893 Msa0430370:CDS
GAGTGACTGGTAGTGTTTAT+TGG 0.371943 3_2:+82179054 None:intergenic
GAAGCAAATGGACGAGTTGC+AGG 0.380501 3_2:-82176387 Msa0430370:intron
GACGAGTTGCAGGTCATTAA+GGG 0.386548 3_2:-82176377 Msa0430370:CDS
GCGACTCATTTGCAAAGCTT+TGG 0.398902 3_2:-82178799 Msa0430370:CDS
GAAAAGATAGCGGAGAAAAT+GGG 0.401309 3_2:+82178951 None:intergenic
AAACACATATATTTGTTTGC+TGG 0.402147 3_2:+82179309 None:intergenic
TTAATAGTTTAAGGTTATAG+AGG 0.402780 3_2:-82176165 Msa0430370:three_prime_UTR
CGTACAAAATCTGTTCTTGT+TGG 0.403520 3_2:-82178700 Msa0430370:CDS
ATTGCACGTTATCAAATTCA+AGG 0.405029 3_2:+82177679 None:intergenic
GAATGAGTGACACGTAAGAT+TGG 0.412038 3_2:+82179116 None:intergenic
GTGAGAGGAGTGAGAGTGAC+TGG 0.413347 3_2:+82179041 None:intergenic
TCGATGTATTGCTGACCTGC+TGG 0.417680 3_2:-82176040 Msa0430370:three_prime_UTR
TTGGATTAGGTGGTTGGCAT+TGG 0.430118 3_2:-82178651 Msa0430370:CDS
TACTATGTTACATATTAATC+GGG 0.431732 3_2:-82176217 Msa0430370:three_prime_UTR
TTCCCTTACGACCAGTTTCA+AGG 0.436157 3_2:+82176093 None:intergenic
CACAGAATGCATAGATCATT+GGG 0.437599 3_2:-82177138 Msa0430370:CDS
ATTAGGTGGTTGGCATTGGC+TGG 0.438971 3_2:-82178647 Msa0430370:intron
GCACAGAATGCATAGATCAT+TGG 0.439918 3_2:-82177139 Msa0430370:CDS
AATTACTAATATATACAATA+AGG 0.444214 3_2:+82175999 None:intergenic
GGCGTTGTTAAGAAAGCTGT+TGG 0.447387 3_2:-82178670 Msa0430370:CDS
TGACTTGAGCAAGCTTGAAA+TGG 0.450063 3_2:-82176913 Msa0430370:CDS
TTGCAAAGCTTTGGTGGTGG+TGG 0.460216 3_2:-82178790 Msa0430370:CDS
GATATATGTGACCTTGAAAC+TGG 0.460934 3_2:-82176104 Msa0430370:three_prime_UTR
GGTAGTGTTTATTGGTGTGT+TGG 0.462216 3_2:+82179062 None:intergenic
TTGGTTCATTCCCTCTCGTC+GGG 0.468051 3_2:-82179092 Msa0430370:five_prime_UTR
GTTTGCTGGCAGAGTGAAGA+TGG 0.478149 3_2:+82179323 None:intergenic
GCCAATCCGCCACCACCCTA+TGG 0.479576 3_2:-82175917 Msa0430370:three_prime_UTR
ACTCATTTGCAAAGCTTTGG+TGG 0.481146 3_2:-82178796 Msa0430370:CDS
CAACTAGCTGCTCCTTTGAT+GGG 0.486144 3_2:+82176727 None:intergenic
AATGAGTGACACGTAAGATT+GGG 0.487610 3_2:+82179117 None:intergenic
AGACTGTGTGCAAATGAGAT+TGG 0.492330 3_2:-82176328 Msa0430370:exon
TCTGGTGATTCACAGTCTAA+GGG 0.496708 3_2:-82177084 Msa0430370:CDS
AGTTCAGTTAACGGTGGTGG+TGG 0.498339 3_2:-82178826 Msa0430370:CDS
ATCATCTTCTTCATTGCCAG+TGG 0.499955 3_2:+82177704 None:intergenic
GGGTCCGTTACTATGCCTCA+TGG 0.500458 3_2:-82177063 Msa0430370:CDS
GCCATAGGGTGGTGGCGGAT+TGG 0.502890 3_2:+82175916 None:intergenic
AAGCTGTTGGATTAGGTGGT+TGG 0.508473 3_2:-82178657 Msa0430370:CDS
ATTGGTTCATTCCCTCTCGT+CGG 0.508541 3_2:-82179093 Msa0430370:five_prime_UTR
GCAATGGAAAGTTCAGTTAA+CGG 0.508721 3_2:-82178835 Msa0430370:CDS
ACCATGAGTAGGTCCTTCTC+TGG 0.512079 3_2:-82177102 Msa0430370:CDS
TGTGTTACCTCGTCATACTA+AGG 0.520326 3_2:-82178743 Msa0430370:CDS
TACTATGCCTCATGGGTCCA+TGG 0.522112 3_2:-82177055 Msa0430370:intron
TTGAATTTGATAACGTGCAA+TGG 0.522138 3_2:-82177677 Msa0430370:CDS
ATAACCACTTCATTAGCCAT+AGG 0.525655 3_2:+82175901 None:intergenic
TGACCTTGAAACTGGTCGTA+AGG 0.527053 3_2:-82176096 Msa0430370:three_prime_UTR
CATTTGCAAAGCTTTGGTGG+TGG 0.527791 3_2:-82178793 Msa0430370:CDS
AAAAGATAGCGGAGAAAATG+GGG 0.534955 3_2:+82178952 None:intergenic
AGACGAAATGATTGAAGCAA+TGG 0.538460 3_2:-82178851 Msa0430370:exon
GAGGCATTTCATGTCCCAGC+TGG 0.539302 3_2:-82176694 Msa0430370:intron
CAATGGAATGGATCAGATAT+GGG 0.541429 3_2:-82177660 Msa0430370:intron
GAAGTGGTTATTAATTACCT+TGG 0.541932 3_2:-82175889 Msa0430370:three_prime_UTR
CATTAGCCATAGGGTGGTGG+CGG 0.545825 3_2:+82175911 None:intergenic
CTAAAAGCTCACTTCCCAGC+AGG 0.547536 3_2:+82176025 None:intergenic
GAACATCGAAAAGAAAGCCT+TGG 0.547931 3_2:+82176066 None:intergenic
TGTGCGAATTTCATGAGTAA+AGG 0.552018 3_2:+82178927 None:intergenic
TAACCACTTCATTAGCCATA+GGG 0.553236 3_2:+82175902 None:intergenic
AAGAAAGCTGTTGGATTAGG+TGG 0.556533 3_2:-82178661 Msa0430370:CDS
ATTTGTCAATGCTGCAAAGA+GGG 0.558483 3_2:-82176354 Msa0430370:CDS
TTTGATAACGTGCAATGGAA+TGG 0.564949 3_2:-82177672 Msa0430370:CDS
CTTCATTAGCCATAGGGTGG+TGG 0.564958 3_2:+82175908 None:intergenic
GACACGTAAGATTGGGAAGG+TGG 0.565769 3_2:+82179124 None:intergenic
TCATCTTCTTCATTGCCAGT+GGG 0.573087 3_2:+82177705 None:intergenic
AGCGTGATTGAAGCACCCAC+TGG 0.574928 3_2:-82177720 Msa0430370:CDS
GACCTTGAAACTGGTCGTAA+GGG 0.580014 3_2:-82176095 Msa0430370:three_prime_UTR
GGTCGTAAGGGAAGTCTCCA+AGG 0.583097 3_2:-82176083 Msa0430370:three_prime_UTR
CCACCCTATGGCTAATGAAG+TGG 0.584364 3_2:-82175905 Msa0430370:three_prime_UTR
GAAAGTTCAGTTAACGGTGG+TGG 0.587165 3_2:-82178829 Msa0430370:CDS
AAGAAGAGAGAGAAAGTGAG+AGG 0.587528 3_2:+82179026 None:intergenic
CGATGTATTGCTGACCTGCT+GGG 0.594454 3_2:-82176039 Msa0430370:three_prime_UTR
TGTCGTCCCAAACCCATCAA+AGG 0.596940 3_2:-82176739 Msa0430370:CDS
GGTCCGTTACTATGCCTCAT+GGG 0.599829 3_2:-82177062 Msa0430370:CDS
CATATATCTACATAATAACT+GGG 0.605713 3_2:+82176119 None:intergenic
AGACTGTGAATCACCAGAGA+AGG 0.609518 3_2:+82177089 None:intergenic
ACCAGAGAAGGACCTACTCA+TGG 0.614361 3_2:+82177101 None:intergenic
GAATTGAAGTGAAGCTACAA+AGG 0.619531 3_2:-82177752 Msa0430370:CDS
CTGGTGATTCACAGTCTAAG+GGG 0.620120 3_2:-82177083 Msa0430370:CDS
AAACCAACCACGCAAACGCA+AGG 0.625045 3_2:-82179378 None:intergenic
TGGTATGAATCCCGACGAGA+GGG 0.629840 3_2:+82179082 None:intergenic
GAGAGAGACTAGAGAGAGAG+CGG 0.630294 3_2:+82179175 None:intergenic
ATCATAGAACGGTCTTACCA+AGG 0.631889 3_2:+82175872 None:intergenic
TGTAACATAGTAAGTACGAA+GGG 0.633296 3_2:+82176228 None:intergenic
AGCTAGTTGATGTTGTTCAG+AGG 0.634780 3_2:-82176713 Msa0430370:CDS
ACACGTAAGATTGGGAAGGT+GGG 0.639779 3_2:+82179125 None:intergenic
TTGGTATGAATCCCGACGAG+AGG 0.642355 3_2:+82179081 None:intergenic
AGCTTGAAATGGCTGCATTG+TGG 0.645923 3_2:-82176902 Msa0430370:CDS
TGGTGGTGATAGTAGTGAAG+AGG 0.648847 3_2:-82178773 Msa0430370:CDS
CACACAAGTCTCACAAATCG+AGG 0.652726 3_2:-82177161 Msa0430370:CDS
GATTTGTCAATGCTGCAAAG+AGG 0.660589 3_2:-82176355 Msa0430370:CDS
AAAGATAGCGGAGAAAATGG+GGG 0.664645 3_2:+82178953 None:intergenic
GAGTAAAGGTGAAAAGATAG+CGG 0.665485 3_2:+82178941 None:intergenic
ATGTAACATAGTAAGTACGA+AGG 0.675651 3_2:+82176227 None:intergenic
AGTGACACGTAAGATTGGGA+AGG 0.686314 3_2:+82179121 None:intergenic
GGACCCATGAGGCATAGTAA+CGG 0.688810 3_2:+82177059 None:intergenic
CCACTTCATTAGCCATAGGG+TGG 0.692185 3_2:+82175905 None:intergenic
AACATAGTAAGTACGAAGGG+AGG 0.698574 3_2:+82176231 None:intergenic
CATGTCACAAGACCATGAGT+AGG 0.700516 3_2:-82177113 Msa0430370:CDS
TGATATACCATGGACCCATG+AGG 0.707981 3_2:+82177048 None:intergenic
ATATCTACATAATAACTGGG+TGG 0.711276 3_2:+82176122 None:intergenic
ACAACAACCTTAGTATGACG+AGG 0.714849 3_2:+82178736 None:intergenic
ATGGAAAGTTCAGTTAACGG+TGG 0.729999 3_2:-82178832 Msa0430370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTCTATTTTTTATTATTA+AGG - chr3_2:82176591-82176610 Msa0430370:intron 10.0%
!!! ATTGTTTATTAAGTTTATTT+TGG - chr3_2:82177047-82177066 Msa0430370:intron 10.0%
!!! TTGTTTATTAAGTTTATTTT+GGG - chr3_2:82177048-82177067 Msa0430370:intron 10.0%
!! TAACACAATTTATTTAATAT+TGG + chr3_2:82177132-82177151 None:intergenic 10.0%
!! TATTAAATAAATTGTGTTAT+TGG - chr3_2:82177133-82177152 Msa0430370:CDS 10.0%
!!! AATTTAGATTATTTGAAATT+AGG - chr3_2:82177359-82177378 Msa0430370:intron 10.0%
!! ATTGTTAAAAAAAATCATTT+AGG + chr3_2:82177385-82177404 None:intergenic 10.0%
!! ATAATTATTAAAAATAGTAC+CGG + chr3_2:82177966-82177985 None:intergenic 10.0%
!! AATTACTAATATATACAATA+AGG + chr3_2:82179196-82179215 None:intergenic 10.0%
!!! AATTTTTTTTTGTCCTTTTT+TGG - chr3_2:82175920-82175939 Msa0430370:three_prime_UTR 15.0%
!!! TGTTTATTAAGTTTATTTTG+GGG - chr3_2:82177049-82177068 Msa0430370:intron 15.0%
!!! ATATGCATTTTTATTTTTCA+TGG - chr3_2:82177206-82177225 Msa0430370:intron 15.0%
!! TTATTAATAATAATTCTGAC+TGG - chr3_2:82177586-82177605 Msa0430370:intron 15.0%
!!! TAGTTTATTAATTGTTGTTT+TGG - chr3_2:82177800-82177819 Msa0430370:intron 15.0%
!! TGGTATATATAGTAAAAAAA+AGG + chr3_2:82177896-82177915 None:intergenic 15.0%
!! TATCAGATAATTTACAAATT+CGG + chr3_2:82178672-82178691 None:intergenic 15.0%
!! TTACTATGTTACATATTAAT+CGG - chr3_2:82178974-82178993 Msa0430370:five_prime_UTR 15.0%
!!! CATTTCATTTCTAATTTTCT+GGG + chr3_2:82175992-82176011 None:intergenic 20.0%
!!! TCATTTCATTTCTAATTTTC+TGG + chr3_2:82175993-82176012 None:intergenic 20.0%
!! TGAAATTGAAATGATAAGAA+TGG + chr3_2:82176639-82176658 None:intergenic 20.0%
!!! TAACCGTTTTTTTTTTCTTT+CGG - chr3_2:82176764-82176783 Msa0430370:intron 20.0%
!! AAATCAAAACTTGAATGATT+TGG + chr3_2:82176838-82176857 None:intergenic 20.0%
!!! AATTTTGATTCTCAAAATGT+TGG - chr3_2:82176865-82176884 Msa0430370:intron 20.0%
!!! ATTTTGATTCTCAAAATGTT+GGG - chr3_2:82176866-82176885 Msa0430370:intron 20.0%
!! ATATTGGAAAATAATACCAA+AGG + chr3_2:82177116-82177135 None:intergenic 20.0%
!! GTTAATCATTATTATTGAGA+TGG + chr3_2:82177665-82177684 None:intergenic 20.0%
!!! TCCTATAATAACTAATCTTT+TGG - chr3_2:82178343-82178362 Msa0430370:intron 20.0%
!!! ACCAAAAGATTAGTTATTAT+AGG + chr3_2:82178347-82178366 None:intergenic 20.0%
!!! CTTAGTACTTACTTTTAAAA+TGG - chr3_2:82178417-82178436 Msa0430370:intron 20.0%
!!! AAGATAGAAACTTGATAATA+TGG + chr3_2:82178635-82178654 None:intergenic 20.0%
!! TACTATGTTACATATTAATC+GGG - chr3_2:82178975-82178994 Msa0430370:five_prime_UTR 20.0%
!!! TTAATAGTTTAAGGTTATAG+AGG - chr3_2:82179027-82179046 Msa0430370:five_prime_UTR 20.0%
!! CATATATCTACATAATAACT+GGG + chr3_2:82179076-82179095 None:intergenic 20.0%
!! ACATATATCTACATAATAAC+TGG + chr3_2:82179077-82179096 None:intergenic 20.0%
!! AAAAACTTCAAATCATAGAA+CGG + chr3_2:82179334-82179353 None:intergenic 20.0%
!!! TTGTTGTTATCAAAAGTATT+AGG - chr3_2:82179360-82179379 Msa0430370:five_prime_UTR 20.0%
! AAACACATATATTTGTTTGC+TGG + chr3_2:82175886-82175905 None:intergenic 25.0%
!!! TTTTTTTTCCTTTTTCTTCG+TGG + chr3_2:82175955-82175974 None:intergenic 25.0%
!!! AACCGTTTTTTTTTTCTTTC+GGG - chr3_2:82176765-82176784 Msa0430370:intron 25.0%
! AACCCGAAAGAAAAAAAAAA+CGG + chr3_2:82176770-82176789 None:intergenic 25.0%
! GGATAAGAATTCATAAAGTA+GGG + chr3_2:82176817-82176836 None:intergenic 25.0%
! TGGATAAGAATTCATAAAGT+AGG + chr3_2:82176818-82176837 None:intergenic 25.0%
!!! TTTTGATTCTCAAAATGTTG+GGG - chr3_2:82176867-82176886 Msa0430370:intron 25.0%
!!! ATTTTTTGCTGTTGTTTATG+TGG - chr3_2:82176917-82176936 Msa0430370:CDS 25.0%
!!! TTTTTTGCTGTTGTTTATGT+GGG - chr3_2:82176918-82176937 Msa0430370:CDS 25.0%
!! ATTGGATTTTCTGAATCTAA+TGG - chr3_2:82177151-82177170 Msa0430370:CDS 25.0%
!! TTGGATTTTCTGAATCTAAT+GGG - chr3_2:82177152-82177171 Msa0430370:CDS 25.0%
!! AATCTAATGGGGATTTTATT+TGG - chr3_2:82177164-82177183 Msa0430370:CDS 25.0%
!!! ATCTAATGGGGATTTTATTT+GGG - chr3_2:82177165-82177184 Msa0430370:CDS 25.0%
! TGCAATTAACTAATTATCAG+TGG - chr3_2:82177265-82177284 Msa0430370:intron 25.0%
! TTTAGCTGTATAAATAATGC+AGG - chr3_2:82177405-82177424 Msa0430370:intron 25.0%
!! CTGTTCTGTTTTATCTTAAA+GGG - chr3_2:82177614-82177633 Msa0430370:intron 25.0%
! TCATTATTATTGAGATGGAA+TGG + chr3_2:82177660-82177679 None:intergenic 25.0%
! AGTTGAATCACATTAATTCT+AGG + chr3_2:82177713-82177732 None:intergenic 25.0%
!!! TATTAAAAATAGTACCGGTA+GGG + chr3_2:82177961-82177980 None:intergenic 25.0%
!!! TTATTAAAAATAGTACCGGT+AGG + chr3_2:82177962-82177981 None:intergenic 25.0%
! TATCTTATTGAAGATGCTTA+TGG - chr3_2:82178372-82178391 Msa0430370:intron 25.0%
!!! TACTTTTAAAATGGATTTGC+AGG - chr3_2:82178426-82178445 Msa0430370:intron 25.0%
!! ATAATTTTTATGTACCAGCT+GGG + chr3_2:82178515-82178534 None:intergenic 25.0%
!! AATAATTTTTATGTACCAGC+TGG + chr3_2:82178516-82178535 None:intergenic 25.0%
!! ACAAGAGTTCTTTATTTCAA+TGG + chr3_2:82178569-82178588 None:intergenic 25.0%
! AATTTGAACTGTTGTAATTG+AGG - chr3_2:82178591-82178610 Msa0430370:intron 25.0%
!! AAGTTCTACAACAAACTTTT+AGG + chr3_2:82178723-82178742 None:intergenic 25.0%
!! CATATTTCTGATATAGTAGT+AGG - chr3_2:82179004-82179023 Msa0430370:five_prime_UTR 25.0%
! AGTAGTAGGTTAATAGTTTA+AGG - chr3_2:82179018-82179037 Msa0430370:five_prime_UTR 25.0%
! CAACAAGAACAGATTTTGTA+CGG + chr3_2:82176494-82176513 None:intergenic 30.0%
! TTCAAACCATTTTCTTGTGT+CGG - chr3_2:82176655-82176674 Msa0430370:intron 30.0%
TGCATGTTTCTCTTTATTAC+TGG - chr3_2:82176982-82177001 Msa0430370:intron 30.0%
TTTCTCTTTATTACTGGATC+TGG - chr3_2:82176988-82177007 Msa0430370:intron 30.0%
AAAATAATACCAAAGGAGGT+AGG + chr3_2:82177109-82177128 None:intergenic 30.0%
TTGGAAAATAATACCAAAGG+AGG + chr3_2:82177113-82177132 None:intergenic 30.0%
! TGGATTTTCTGAATCTAATG+GGG - chr3_2:82177153-82177172 Msa0430370:CDS 30.0%
ATTGAAATCAAGTTTGTCGT+AGG - chr3_2:82177289-82177308 Msa0430370:intron 30.0%
ATAATGCAGGTTCTTACTAA+TGG - chr3_2:82177418-82177437 Msa0430370:intron 30.0%
ATTGCACGTTATCAAATTCA+AGG + chr3_2:82177516-82177535 None:intergenic 30.0%
!! TTGAATTTGATAACGTGCAA+TGG - chr3_2:82177515-82177534 Msa0430370:intron 30.0%
! GCTGTTCTGTTTTATCTTAA+AGG - chr3_2:82177613-82177632 Msa0430370:intron 30.0%
! TATTTATTTGTTGACCCTAC+CGG - chr3_2:82177944-82177963 Msa0430370:intron 30.0%
! GATTTAAAGCTGATATACCA+TGG + chr3_2:82178157-82178176 None:intergenic 30.0%
AAAATTATCTAATGCGTCAG+TGG + chr3_2:82178202-82178221 None:intergenic 30.0%
! CTTTGTATATTTTCTGCAGA+AGG - chr3_2:82178255-82178274 Msa0430370:intron 30.0%
!!! TTTTAAAATGGATTTGCAGG+TGG - chr3_2:82178429-82178448 Msa0430370:intron 30.0%
CAAGAAAGAAACACAATTTC+AGG + chr3_2:82178784-82178803 None:intergenic 30.0%
TTTCTTTCTTGTGAAGCAAA+TGG - chr3_2:82178793-82178812 Msa0430370:CDS 30.0%
TGTAACATAGTAAGTACGAA+GGG + chr3_2:82178967-82178986 None:intergenic 30.0%
ATGTAACATAGTAAGTACGA+AGG + chr3_2:82178968-82178987 None:intergenic 30.0%
! AGGTACTAATTACTCTTTTG+TGG - chr3_2:82179047-82179066 Msa0430370:five_prime_UTR 30.0%
ATATCTACATAATAACTGGG+TGG + chr3_2:82179073-82179092 None:intergenic 30.0%
GAAGTGGTTATTAATTACCT+TGG - chr3_2:82179303-82179322 Msa0430370:five_prime_UTR 30.0%
AATGAGTGACACGTAAGATT+GGG + chr3_2:82176078-82176097 None:intergenic 35.0%
!! AAAAGATAGCGGAGAAAATG+GGG + chr3_2:82176243-82176262 None:intergenic 35.0%
!! GAAAAGATAGCGGAGAAAAT+GGG + chr3_2:82176244-82176263 None:intergenic 35.0%
!! TGAAAAGATAGCGGAGAAAA+TGG + chr3_2:82176245-82176264 None:intergenic 35.0%
GAGTAAAGGTGAAAAGATAG+CGG + chr3_2:82176254-82176273 None:intergenic 35.0%
TGTGCGAATTTCATGAGTAA+AGG + chr3_2:82176268-82176287 None:intergenic 35.0%
AGACGAAATGATTGAAGCAA+TGG - chr3_2:82176341-82176360 Msa0430370:CDS 35.0%
GCAATGGAAAGTTCAGTTAA+CGG - chr3_2:82176357-82176376 Msa0430370:CDS 35.0%
! CATACTAAGGTTGTTGTTAC+TGG - chr3_2:82176462-82176481 Msa0430370:intron 35.0%
CGTACAAAATCTGTTCTTGT+TGG - chr3_2:82176492-82176511 Msa0430370:intron 35.0%
GTTAAGAAAGCTGTTGGATT+AGG - chr3_2:82176528-82176547 Msa0430370:intron 35.0%
AATTGACCGACACAAGAAAA+TGG + chr3_2:82176664-82176683 None:intergenic 35.0%
!! TTTCTTGTGTCGGTCAATTT+TGG - chr3_2:82176665-82176684 Msa0430370:intron 35.0%
GAGAAACATGCAATGAATAC+AGG + chr3_2:82176974-82176993 None:intergenic 35.0%
AGTATATTACCTACCTCCTT+TGG - chr3_2:82177097-82177116 Msa0430370:CDS 35.0%
GAATTGAAGTGAAGCTACAA+AGG - chr3_2:82177440-82177459 Msa0430370:intron 35.0%
!! TTTGATAACGTGCAATGGAA+TGG - chr3_2:82177520-82177539 Msa0430370:intron 35.0%
CAATGGAATGGATCAGATAT+GGG - chr3_2:82177532-82177551 Msa0430370:intron 35.0%
CAGAAACAAGTACTAGTACT+CGG + chr3_2:82177750-82177769 None:intergenic 35.0%
GTTTGCAAAGTGATAGAGTT+TGG - chr3_2:82177838-82177857 Msa0430370:intron 35.0%
! GTGATAGAGTTTGGTAACAT+TGG - chr3_2:82177847-82177866 Msa0430370:intron 35.0%
GGAAAAAAAGACACGCATAA+AGG + chr3_2:82177875-82177894 None:intergenic 35.0%
! GCAAAACACTAGAGTCTTTT+TGG + chr3_2:82177916-82177935 None:intergenic 35.0%
CACAGAATGCATAGATCATT+GGG - chr3_2:82178054-82178073 Msa0430370:intron 35.0%
TACAAATTCGGCGTAATTTG+TGG + chr3_2:82178660-82178679 None:intergenic 35.0%
CTGATATGCATGAATTGTGA+TGG - chr3_2:82178686-82178705 Msa0430370:CDS 35.0%
ATTTGTCAATGCTGCAAAGA+GGG - chr3_2:82178838-82178857 Msa0430370:CDS 35.0%
GATATATGTGACCTTGAAAC+TGG - chr3_2:82179088-82179107 Msa0430370:five_prime_UTR 35.0%
TAACCACTTCATTAGCCATA+GGG + chr3_2:82179293-82179312 None:intergenic 35.0%
ATAACCACTTCATTAGCCAT+AGG + chr3_2:82179294-82179313 None:intergenic 35.0%
GTGGCATTGTGATCCAAAAA+AGG + chr3_2:82175936-82175955 None:intergenic 40.0%
TCACAATGCCACGAAGAAAA+AGG - chr3_2:82175944-82175963 Msa0430370:three_prime_UTR 40.0%
GAATGAGTGACACGTAAGAT+TGG + chr3_2:82176079-82176098 None:intergenic 40.0%
! CTTACGTGTCACTCATTCAT+TGG - chr3_2:82176081-82176100 Msa0430370:three_prime_UTR 40.0%
GGTAGTGTTTATTGGTGTGT+TGG + chr3_2:82176133-82176152 None:intergenic 40.0%
! GAGTGACTGGTAGTGTTTAT+TGG + chr3_2:82176141-82176160 None:intergenic 40.0%
AAGAAGAGAGAGAAAGTGAG+AGG + chr3_2:82176169-82176188 None:intergenic 40.0%
!! AAAGATAGCGGAGAAAATGG+GGG + chr3_2:82176242-82176261 None:intergenic 40.0%
ATGGAAAGTTCAGTTAACGG+TGG - chr3_2:82176360-82176379 Msa0430370:CDS 40.0%
! ACTCATTTGCAAAGCTTTGG+TGG - chr3_2:82176396-82176415 Msa0430370:intron 40.0%
TGTGTTACCTCGTCATACTA+AGG - chr3_2:82176449-82176468 Msa0430370:intron 40.0%
ACAACAACCTTAGTATGACG+AGG + chr3_2:82176459-82176478 None:intergenic 40.0%
!! TCTGTTCTTGTTGGTCTTCA+AGG - chr3_2:82176501-82176520 Msa0430370:intron 40.0%
! AAGAAAGCTGTTGGATTAGG+TGG - chr3_2:82176531-82176550 Msa0430370:intron 40.0%
TCATCTTCTTCATTGCCAGT+GGG + chr3_2:82177490-82177509 None:intergenic 40.0%
ATCATCTTCTTCATTGCCAG+TGG + chr3_2:82177491-82177510 None:intergenic 40.0%
GCAATGGAATGGATCAGATA+TGG - chr3_2:82177531-82177550 Msa0430370:intron 40.0%
TCTATCACTTTGCAAACCCA+TGG + chr3_2:82177835-82177854 None:intergenic 40.0%
GCACAGAATGCATAGATCAT+TGG - chr3_2:82178053-82178072 Msa0430370:intron 40.0%
TCTGGTGATTCACAGTCTAA+GGG - chr3_2:82178108-82178127 Msa0430370:intron 40.0%
TATCTAATGCGTCAGTGGTA+GGG + chr3_2:82178197-82178216 None:intergenic 40.0%
TTATCTAATGCGTCAGTGGT+AGG + chr3_2:82178198-82178217 None:intergenic 40.0%
TGACTTGAGCAAGCTTGAAA+TGG - chr3_2:82178279-82178298 Msa0430370:intron 40.0%
! AGCTAGTTGATGTTGTTCAG+AGG - chr3_2:82178479-82178498 Msa0430370:intron 40.0%
GATTTGTCAATGCTGCAAAG+AGG - chr3_2:82178837-82178856 Msa0430370:CDS 40.0%
AGACTGTGTGCAAATGAGAT+TGG - chr3_2:82178864-82178883 Msa0430370:exon 40.0%
AACATAGTAAGTACGAAGGG+AGG + chr3_2:82178964-82178983 None:intergenic 40.0%
GAACATCGAAAAGAAAGCCT+TGG + chr3_2:82179129-82179148 None:intergenic 40.0%
ATCATAGAACGGTCTTACCA+AGG + chr3_2:82179323-82179342 None:intergenic 40.0%
ACACGTAAGATTGGGAAGGT+GGG + chr3_2:82176070-82176089 None:intergenic 45.0%
AGTGACACGTAAGATTGGGA+AGG + chr3_2:82176074-82176093 None:intergenic 45.0%
! ATTGGTTCATTCCCTCTCGT+CGG - chr3_2:82176099-82176118 Msa0430370:three_prime_UTR 45.0%
GGAGAAAATGGGGGAAATGT+AGG + chr3_2:82176233-82176252 None:intergenic 45.0%
GAAAGTTCAGTTAACGGTGG+TGG - chr3_2:82176363-82176382 Msa0430370:CDS 45.0%
GCGACTCATTTGCAAAGCTT+TGG - chr3_2:82176393-82176412 Msa0430370:intron 45.0%
! CATTTGCAAAGCTTTGGTGG+TGG - chr3_2:82176399-82176418 Msa0430370:intron 45.0%
! TGGTGGTGATAGTAGTGAAG+AGG - chr3_2:82176419-82176438 Msa0430370:intron 45.0%
GGCGTTGTTAAGAAAGCTGT+TGG - chr3_2:82176522-82176541 Msa0430370:intron 45.0%
! AAGCTGTTGGATTAGGTGGT+TGG - chr3_2:82176535-82176554 Msa0430370:intron 45.0%
! TTGGATTAGGTGGTTGGCAT+TGG - chr3_2:82176541-82176560 Msa0430370:intron 45.0%
!!! TGTTTTGGCTTCCATGACCA+TGG - chr3_2:82177815-82177834 Msa0430370:intron 45.0%
!!! GTTTTGGCTTCCATGACCAT+GGG - chr3_2:82177816-82177835 Msa0430370:intron 45.0%
ACTTTGCAAACCCATGGTCA+TGG + chr3_2:82177829-82177848 None:intergenic 45.0%
CACACAAGTCTCACAAATCG+AGG - chr3_2:82178031-82178050 Msa0430370:intron 45.0%
CATGTCACAAGACCATGAGT+AGG - chr3_2:82178079-82178098 Msa0430370:intron 45.0%
AGACTGTGAATCACCAGAGA+AGG + chr3_2:82178106-82178125 None:intergenic 45.0%
CTCTGGTGATTCACAGTCTA+AGG - chr3_2:82178107-82178126 Msa0430370:intron 45.0%
CTGGTGATTCACAGTCTAAG+GGG - chr3_2:82178109-82178128 Msa0430370:intron 45.0%
TGATATACCATGGACCCATG+AGG + chr3_2:82178147-82178166 None:intergenic 45.0%
AGCTTGAAATGGCTGCATTG+TGG - chr3_2:82178290-82178309 Msa0430370:intron 45.0%
TGGCTGCATTGTGGAGATAT+TGG - chr3_2:82178299-82178318 Msa0430370:intron 45.0%
CAACTAGCTGCTCCTTTGAT+GGG + chr3_2:82178468-82178487 None:intergenic 45.0%
TCAACTAGCTGCTCCTTTGA+TGG + chr3_2:82178469-82178488 None:intergenic 45.0%
GACGAGTTGCAGGTCATTAA+GGG - chr3_2:82178815-82178834 Msa0430370:CDS 45.0%
TGACCTTGAAACTGGTCGTA+AGG - chr3_2:82179096-82179115 Msa0430370:five_prime_UTR 45.0%
GACCTTGAAACTGGTCGTAA+GGG - chr3_2:82179097-82179116 Msa0430370:five_prime_UTR 45.0%
TTCCCTTACGACCAGTTTCA+AGG + chr3_2:82179102-82179121 None:intergenic 45.0%
!! CATTAATAATTAAAATATTA+AGG + chr3_2:82178228-82178247 None:intergenic 5.0%
! GTTTGCTGGCAGAGTGAAGA+TGG + chr3_2:82175872-82175891 None:intergenic 50.0%
GAGAGAGACTAGAGAGAGAG+CGG + chr3_2:82176020-82176039 None:intergenic 50.0%
GACACGTAAGATTGGGAAGG+TGG + chr3_2:82176071-82176090 None:intergenic 50.0%
TTGGTTCATTCCCTCTCGTC+GGG - chr3_2:82176100-82176119 Msa0430370:three_prime_UTR 50.0%
TGGTATGAATCCCGACGAGA+GGG + chr3_2:82176113-82176132 None:intergenic 50.0%
TTGGTATGAATCCCGACGAG+AGG + chr3_2:82176114-82176133 None:intergenic 50.0%
! AGTTCAGTTAACGGTGGTGG+TGG - chr3_2:82176366-82176385 Msa0430370:CDS 50.0%
!! TTGCAAAGCTTTGGTGGTGG+TGG - chr3_2:82176402-82176421 Msa0430370:intron 50.0%
!! ATTAGGTGGTTGGCATTGGC+TGG - chr3_2:82176545-82176564 Msa0430370:intron 50.0%
ACCATGAGTAGGTCCTTCTC+TGG - chr3_2:82178090-82178109 Msa0430370:intron 50.0%
ACCAGAGAAGGACCTACTCA+TGG + chr3_2:82178094-82178113 None:intergenic 50.0%
GGTCCGTTACTATGCCTCAT+GGG - chr3_2:82178130-82178149 Msa0430370:intron 50.0%
GGACCCATGAGGCATAGTAA+CGG + chr3_2:82178136-82178155 None:intergenic 50.0%
TACTATGCCTCATGGGTCCA+TGG - chr3_2:82178137-82178156 Msa0430370:intron 50.0%
TGTCGTCCCAAACCCATCAA+AGG - chr3_2:82178453-82178472 Msa0430370:intron 50.0%
!! GCTGCTCCTTTGATGGGTTT+GGG + chr3_2:82178462-82178481 None:intergenic 50.0%
!! AGCTGCTCCTTTGATGGGTT+TGG + chr3_2:82178463-82178482 None:intergenic 50.0%
GAAGCAAATGGACGAGTTGC+AGG - chr3_2:82178805-82178824 Msa0430370:CDS 50.0%
GGACGAGTTGCAGGTCATTA+AGG - chr3_2:82178814-82178833 Msa0430370:CDS 50.0%
TCGATGTATTGCTGACCTGC+TGG - chr3_2:82179152-82179171 Msa0430370:five_prime_UTR 50.0%
CGATGTATTGCTGACCTGCT+GGG - chr3_2:82179153-82179172 Msa0430370:five_prime_UTR 50.0%
CTAAAAGCTCACTTCCCAGC+AGG + chr3_2:82179170-82179189 None:intergenic 50.0%
CTTCATTAGCCATAGGGTGG+TGG + chr3_2:82179287-82179306 None:intergenic 50.0%
CCACTTCATTAGCCATAGGG+TGG + chr3_2:82179290-82179309 None:intergenic 50.0%
CCACCCTATGGCTAATGAAG+TGG - chr3_2:82179287-82179306 Msa0430370:five_prime_UTR 50.0%
GTGAGAGGAGTGAGAGTGAC+TGG + chr3_2:82176154-82176173 None:intergenic 55.0%
! AGCGTGATTGAAGCACCCAC+TGG - chr3_2:82177472-82177491 Msa0430370:intron 55.0%
GGGTCCGTTACTATGCCTCA+TGG - chr3_2:82178129-82178148 Msa0430370:intron 55.0%
! GAGGCATTTCATGTCCCAGC+TGG - chr3_2:82178498-82178517 Msa0430370:intron 55.0%
GGTCGTAAGGGAAGTCTCCA+AGG - chr3_2:82179109-82179128 Msa0430370:five_prime_UTR 55.0%
! CATTAGCCATAGGGTGGTGG+CGG + chr3_2:82179284-82179303 None:intergenic 55.0%
GCCAATCCGCCACCACCCTA+TGG - chr3_2:82179275-82179294 Msa0430370:five_prime_UTR 65.0%
!! GCCATAGGGTGGTGGCGGAT+TGG + chr3_2:82179279-82179298 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 82175825 82179389 82175825 ID=Msa0430370;Name=Msa0430370
chr3_2 mRNA 82175825 82179389 82175825 ID=Msa0430370-mRNA-1;Parent=Msa0430370;Name=Msa0430370-mRNA-1;_AED=0.12;_eAED=0.12;_QI=523|1|1|1|1|1|6|509|230
chr3_2 exon 82175825 82176405 82175825 ID=Msa0430370-mRNA-1:exon:22772;Parent=Msa0430370-mRNA-1
chr3_2 exon 82176695 82176766 82176695 ID=Msa0430370-mRNA-1:exon:22771;Parent=Msa0430370-mRNA-1
chr3_2 exon 82176878 82176940 82176878 ID=Msa0430370-mRNA-1:exon:22770;Parent=Msa0430370-mRNA-1
chr3_2 exon 82177056 82177195 82177056 ID=Msa0430370-mRNA-1:exon:22769;Parent=Msa0430370-mRNA-1
chr3_2 exon 82177661 82177787 82177661 ID=Msa0430370-mRNA-1:exon:22768;Parent=Msa0430370-mRNA-1
chr3_2 exon 82178648 82179389 82178648 ID=Msa0430370-mRNA-1:exon:22767;Parent=Msa0430370-mRNA-1
chr3_2 five_prime_UTR 82178867 82179389 82178867 ID=Msa0430370-mRNA-1:five_prime_utr;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82178648 82178866 82178648 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82177661 82177787 82177661 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82177056 82177195 82177056 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82176878 82176940 82176878 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82176695 82176766 82176695 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 CDS 82176334 82176405 82176334 ID=Msa0430370-mRNA-1:cds;Parent=Msa0430370-mRNA-1
chr3_2 three_prime_UTR 82175825 82176333 82175825 ID=Msa0430370-mRNA-1:three_prime_utr;Parent=Msa0430370-mRNA-1
Gene Sequence

>Msa0430370

ATGATTGAAGCAATGGAAAGTTCAGTTAACGGTGGTGGTGGTTTCGCGACTCATTTGCAAAGCTTTGGTGGTGGTGGTGATAGTAGTGAAGAGGAACTGTCTGTGTTACCTCGTCATACTAAGGTTGTTGTTACTGGAAATAACCGTACAAAATCTGTTCTTGTTGGTCTTCAAGGCGTTGTTAAGAAAGCTGTTGGATTAGGTGGTTGGCATTGGCTGGTTCTTACTAATGGAATTGAAGTGAAGCTACAAAGGAATGCTCTTAGCGTGATTGAAGCACCCACTGGCAATGAAGAAGATGATGACCTTGAATTTGATAACGTGCAATGGAATGGATCAGATATGGCATCAGACGATACACACAAGTCTCACAAATCGAGGCACAGAATGCATAGATCATTGGGATCATGTCACAAGACCATGAGTAGGTCCTTCTCTGGTGATTCACAGTCTAAGGGGTCCGTTACTATGCCTCATGGGTCCATGAAGGTTGACTTGAGCAAGCTTGAAATGGCTGCATTGTGGAGATATTGGAGACATTTTAATCTTGTGGATGTCGTCCCAAACCCATCAAAGGAGCAGCTAGTTGATGTTGTTCAGAGGCATTTCATGTCCCAGCTGCAAATGGACGAGTTGCAGGTCATTAAGGGATTTGTCAATGCTGCAAAGAGGGTCAAGACTGTGTGCAAATGA
Protein sequence

>Msa0430370

MIEAMESSVNGGGGFATHLQSFGGGGDSSEEELSVLPRHTKVVVTGNNRTKSVLVGLQGVVKKAVGLGGWHWLVLTNGIEVKLQRNALSVIEAPTGNEEDDDLEFDNVQWNGSDMASDDTHKSHKSRHRMHRSLGSCHKTMSRSFSGDSQSKGSVTMPHGSMKVDLSKLEMAALWRYWRHFNLVDVVPNPSKEQLVDVVQRHFMSQLQMDELQVIKGFVNAAKRVKTVCK*