AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0384370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384370 MtrunA17_Chr3g0141391 98.268 231 3 1 1 230 1 231 7.00e-169 464
Msa0384370 MtrunA17_Chr1g0154621 83.117 231 33 4 1 230 1 226 6.38e-136 380
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0384370 AT1G19330.2 76.087 230 52 3 1 230 1 227 3.54e-111 319
Msa0384370 AT1G19330.1 74.894 235 51 4 1 230 1 232 2.47e-109 314
Msa0384370 AT1G19330.3 74.576 236 51 5 1 230 1 233 4.30e-108 311
Msa0384370 AT1G75060.1 69.362 235 65 5 1 230 1 233 1.12e-105 305
Msa0384370 AT1G75060.2 68.936 235 65 6 1 230 1 232 4.11e-103 298
Msa0384370 AT1G75060.3 69.841 189 51 4 1 184 1 188 5.59e-80 238

Find 118 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGTAGGTTAATAGTTTA+AGG 0.109374 3_1:+87416150 Msa0384370:three_prime_UTR
CATACTAAGGTTGTTGTTAC+TGG 0.194013 3_1:+87413562 Msa0384370:CDS
AGCTGCTCCTTTGATGGGTT+TGG 0.231841 3_1:-87415592 None:intergenic
CTCTGGTGAATCACAGTCTA+AGG 0.256419 3_1:+87415239 Msa0384370:CDS
GGACGAGTTGCAGGTCATTA+AGG 0.256826 3_1:+87415946 Msa0384370:CDS
TTACTATGTTACATATTAAT+CGG 0.270714 3_1:+87416106 Msa0384370:three_prime_UTR
ACATATATCTACATAATAAC+TGG 0.282748 3_1:-87416206 None:intergenic
TGAAAAGATAGCGGAGAAAA+TGG 0.292286 3_1:-87413342 None:intergenic
TTGTTGTTATCAAAAGTATT+AGG 0.312640 3_1:+87416492 Msa0384370:three_prime_UTR
TCTGTTCTTGTTGGTCTTCA+AGG 0.325218 3_1:+87413601 Msa0384370:CDS
GTTAAGAAAGCTGTTGGATT+AGG 0.325633 3_1:+87413628 Msa0384370:CDS
GCAATGGAATGGATCAGATA+TGG 0.328027 3_1:+87414631 Msa0384370:CDS
ATAATGCAGGTTCTTACTAA+TGG 0.333844 3_1:+87414518 Msa0384370:intron
GCTGCTCCTTTGATGGGTTT+GGG 0.336272 3_1:-87415591 None:intergenic
CATATTTCTGATATAGTAGT+AGG 0.336697 3_1:+87416136 Msa0384370:three_prime_UTR
TCAACTAGCTGCTCCTTTGA+TGG 0.362105 3_1:-87415598 None:intergenic
CTTACGTGTCACTCATTCAT+TGG 0.366812 3_1:+87413181 Msa0384370:five_prime_UTR
TGGCTGCATTGTGGAGATAT+TGG 0.369691 3_1:+87415431 Msa0384370:CDS
GAGTGACTGGTAGTGTTTAT+TGG 0.371943 3_1:-87413238 None:intergenic
GAAGCAAATGGACGAGTTGC+AGG 0.380501 3_1:+87415937 Msa0384370:intron
GACGAGTTGCAGGTCATTAA+GGG 0.386548 3_1:+87415947 Msa0384370:CDS
GCGACTCATTTGCAAAGCTT+TGG 0.398902 3_1:+87413493 Msa0384370:CDS
GAAAAGATAGCGGAGAAAAT+GGG 0.401309 3_1:-87413341 None:intergenic
AAACACATATATTTGTTTGC+TGG 0.402147 3_1:-87412982 None:intergenic
TTAATAGTTTAAGGTTATAG+AGG 0.402780 3_1:+87416159 Msa0384370:three_prime_UTR
CGTACAAAATCTGTTCTTGT+TGG 0.403520 3_1:+87413592 Msa0384370:CDS
ATTGCACGTTATCAAATTCA+AGG 0.405029 3_1:-87414613 None:intergenic
GAATGAGTGACACGTAAGAT+TGG 0.412038 3_1:-87413176 None:intergenic
GTGAGAGGAGTGAGAGTGAC+TGG 0.413347 3_1:-87413251 None:intergenic
TCGATGTATTGCTGACCTGC+TGG 0.417680 3_1:+87416284 Msa0384370:three_prime_UTR
TTGGATTAGGTGGTTGGCAT+TGG 0.430118 3_1:+87413641 Msa0384370:CDS
TACTATGTTACATATTAATC+GGG 0.431732 3_1:+87416107 Msa0384370:three_prime_UTR
TTCCCTTACGACCAGTTTCA+AGG 0.436157 3_1:-87416231 None:intergenic
CACAGAATGCATAGATCATT+GGG 0.437599 3_1:+87415186 Msa0384370:CDS
ATTAGGTGGTTGGCATTGGC+TGG 0.438971 3_1:+87413645 Msa0384370:CDS
GCACAGAATGCATAGATCAT+TGG 0.439918 3_1:+87415185 Msa0384370:CDS
AATTACTAATATATACAATA+AGG 0.444214 3_1:-87416325 None:intergenic
GGCGTTGTTAAGAAAGCTGT+TGG 0.447387 3_1:+87413622 Msa0384370:CDS
TGACTTGAGCAAGCTTGAAA+TGG 0.450063 3_1:+87415411 Msa0384370:CDS
TTGCAAAGCTTTGGTGGTGG+TGG 0.460216 3_1:+87413502 Msa0384370:CDS
GATATATGTGACCTTGAAAC+TGG 0.460934 3_1:+87416220 Msa0384370:three_prime_UTR
GGTAGTGTTTATTGGTGTGT+TGG 0.462216 3_1:-87413230 None:intergenic
TTGGTTCATTCCCTCTCGTC+GGG 0.468051 3_1:+87413200 Msa0384370:five_prime_UTR
GTTTGCTGGCAGAGTGAAGA+TGG 0.478149 3_1:-87412968 None:intergenic
GCCAATCCGCCACCACCCTA+TGG 0.479576 3_1:+87416407 Msa0384370:three_prime_UTR
ACTCATTTGCAAAGCTTTGG+TGG 0.481146 3_1:+87413496 Msa0384370:CDS
CAACTAGCTGCTCCTTTGAT+GGG 0.486144 3_1:-87415597 None:intergenic
AATGAGTGACACGTAAGATT+GGG 0.487610 3_1:-87413175 None:intergenic
AGACTGTGTGCAAATGAGAT+TGG 0.492330 3_1:+87415996 Msa0384370:exon
AGTTCAGTTAACGGTGGTGG+TGG 0.498339 3_1:+87413466 Msa0384370:CDS
ATCATCTTCTTCATTGCCAG+TGG 0.499955 3_1:-87414588 None:intergenic
GGGTCCGTTACTATGCCTCA+TGG 0.500458 3_1:+87415261 Msa0384370:CDS
GCCATAGGGTGGTGGCGGAT+TGG 0.502890 3_1:-87416408 None:intergenic
AAGCTGTTGGATTAGGTGGT+TGG 0.508473 3_1:+87413635 Msa0384370:CDS
ATTGGTTCATTCCCTCTCGT+CGG 0.508541 3_1:+87413199 Msa0384370:five_prime_UTR
GCAATGGAAAGTTCAGTTAA+CGG 0.508721 3_1:+87413457 Msa0384370:CDS
ACCATGAGTAGGTCCTTCTC+TGG 0.512079 3_1:+87415222 Msa0384370:CDS
TGTGTTACCTCGTCATACTA+AGG 0.520326 3_1:+87413549 Msa0384370:CDS
TACTATGCCTCATGGGTCCA+TGG 0.522112 3_1:+87415269 Msa0384370:CDS
TTGAATTTGATAACGTGCAA+TGG 0.522138 3_1:+87414615 Msa0384370:CDS
ATAACCACTTCATTAGCCAT+AGG 0.525655 3_1:-87416423 None:intergenic
TGACCTTGAAACTGGTCGTA+AGG 0.527053 3_1:+87416228 Msa0384370:three_prime_UTR
CATTTGCAAAGCTTTGGTGG+TGG 0.527791 3_1:+87413499 Msa0384370:CDS
AAAAGATAGCGGAGAAAATG+GGG 0.534955 3_1:-87413340 None:intergenic
AGACGAAATGATTGAAGCAA+TGG 0.538460 3_1:+87413441 Msa0384370:exon
GAGGCATTTCATGTCCCAGC+TGG 0.539302 3_1:+87415630 Msa0384370:CDS
CAATGGAATGGATCAGATAT+GGG 0.541429 3_1:+87414632 Msa0384370:CDS
CATTAGCCATAGGGTGGTGG+CGG 0.545825 3_1:-87416413 None:intergenic
CTAAAAGCTCACTTCCCAGC+AGG 0.547536 3_1:-87416299 None:intergenic
GAACATCGAAAAGAAAGCCT+TGG 0.547931 3_1:-87416258 None:intergenic
TCTGGTGAATCACAGTCTAA+GGG 0.549430 3_1:+87415240 Msa0384370:CDS
TGTGCGAATTTCATGAGTAA+AGG 0.552018 3_1:-87413365 None:intergenic
TAACCACTTCATTAGCCATA+GGG 0.553236 3_1:-87416422 None:intergenic
AAGAAAGCTGTTGGATTAGG+TGG 0.556533 3_1:+87413631 Msa0384370:CDS
ATTTGTCAATGCTGCAAAGA+GGG 0.558483 3_1:+87415970 Msa0384370:CDS
TTTGATAACGTGCAATGGAA+TGG 0.564949 3_1:+87414620 Msa0384370:CDS
CTTCATTAGCCATAGGGTGG+TGG 0.564958 3_1:-87416416 None:intergenic
GACACGTAAGATTGGGAAGG+TGG 0.565769 3_1:-87413168 None:intergenic
TCATCTTCTTCATTGCCAGT+GGG 0.573087 3_1:-87414587 None:intergenic
AGCGTGATTGAAGCACCCAC+TGG 0.574928 3_1:+87414572 Msa0384370:CDS
GACCTTGAAACTGGTCGTAA+GGG 0.580014 3_1:+87416229 Msa0384370:three_prime_UTR
GGTCGTAAGGGAAGTCTCCA+AGG 0.583097 3_1:+87416241 Msa0384370:three_prime_UTR
CCACCCTATGGCTAATGAAG+TGG 0.584364 3_1:+87416419 Msa0384370:three_prime_UTR
GAAAGTTCAGTTAACGGTGG+TGG 0.587165 3_1:+87413463 Msa0384370:CDS
AAGAAGAGAGAGAAAGTGAG+AGG 0.587528 3_1:-87413266 None:intergenic
CGATGTATTGCTGACCTGCT+GGG 0.594454 3_1:+87416285 Msa0384370:three_prime_UTR
TGTCGTCCCAAACCCATCAA+AGG 0.596940 3_1:+87415585 Msa0384370:CDS
GGTCCGTTACTATGCCTCAT+GGG 0.599829 3_1:+87415262 Msa0384370:CDS
AGACTGTGATTCACCAGAGA+AGG 0.602383 3_1:-87415235 None:intergenic
CATATATCTACATAATAACT+GGG 0.605713 3_1:-87416205 None:intergenic
GAAGTGGTTATTAATCACCT+TGG 0.607458 3_1:+87416435 Msa0384370:three_prime_UTR
ACCAGAGAAGGACCTACTCA+TGG 0.614361 3_1:-87415223 None:intergenic
GAATTGAAGTGAAGCTACAA+AGG 0.619531 3_1:+87414540 Msa0384370:CDS
AAACCAACCACGCAAACGCA+AGG 0.625045 3_1:+87412913 None:intergenic
TGGTATGAATCCCGACGAGA+GGG 0.629840 3_1:-87413210 None:intergenic
GAGAGAGACTAGAGAGAGAG+CGG 0.630294 3_1:-87413117 None:intergenic
TGTAACATAGTAAGTACGAA+GGG 0.633296 3_1:-87416096 None:intergenic
AGCTAGTTGATGTTGTTCAG+AGG 0.634780 3_1:+87415611 Msa0384370:CDS
ATCATAGAACGGTCTTACCA+AGG 0.638709 3_1:-87416452 None:intergenic
ACACGTAAGATTGGGAAGGT+GGG 0.639779 3_1:-87413167 None:intergenic
TTGGTATGAATCCCGACGAG+AGG 0.642355 3_1:-87413211 None:intergenic
AGCTTGAAATGGCTGCATTG+TGG 0.645923 3_1:+87415422 Msa0384370:CDS
TGGTGGTGATAGTAGTGAAG+AGG 0.648847 3_1:+87413519 Msa0384370:CDS
CACACAAGTCTCACAAATCG+AGG 0.652726 3_1:+87415163 Msa0384370:CDS
CTGGTGAATCACAGTCTAAG+GGG 0.655308 3_1:+87415241 Msa0384370:CDS
GATTTGTCAATGCTGCAAAG+AGG 0.660589 3_1:+87415969 Msa0384370:CDS
AAAGATAGCGGAGAAAATGG+GGG 0.664645 3_1:-87413339 None:intergenic
GAGTAAAGGTGAAAAGATAG+CGG 0.665485 3_1:-87413351 None:intergenic
ATGTAACATAGTAAGTACGA+AGG 0.675651 3_1:-87416097 None:intergenic
AGTGACACGTAAGATTGGGA+AGG 0.686314 3_1:-87413171 None:intergenic
GGACCCATGAGGCATAGTAA+CGG 0.688810 3_1:-87415265 None:intergenic
CCACTTCATTAGCCATAGGG+TGG 0.692185 3_1:-87416419 None:intergenic
AACATAGTAAGTACGAAGGG+AGG 0.698574 3_1:-87416093 None:intergenic
CATGTCACAAGACCATGAGT+AGG 0.700516 3_1:+87415211 Msa0384370:CDS
TGATATACCATGGACCCATG+AGG 0.707981 3_1:-87415276 None:intergenic
ATATCTACATAATAACTGGG+TGG 0.711276 3_1:-87416202 None:intergenic
ACAACAACCTTAGTATGACG+AGG 0.714849 3_1:-87413556 None:intergenic
ATGGAAAGTTCAGTTAACGG+TGG 0.729999 3_1:+87413460 Msa0384370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTCTATTTTTTATTATTA+AGG + chr3_1:87413691-87413710 Msa0384370:intron 10.0%
!!! ATTGTTTATTAAGTTTATTT+TGG + chr3_1:87414147-87414166 Msa0384370:intron 10.0%
!!! TTGTTTATTAAGTTTATTTT+GGG + chr3_1:87414148-87414167 Msa0384370:intron 10.0%
!! TAACACAATTTATTTAATAT+TGG - chr3_1:87414232-87414251 None:intergenic 10.0%
!! TATTAAATAAATTGTGTTAT+TGG + chr3_1:87414233-87414252 Msa0384370:intron 10.0%
!!! AATTTAGATTATTTGAAATT+AGG + chr3_1:87414459-87414478 Msa0384370:intron 10.0%
!! ATTGTTAAAAAAAATCATTT+AGG - chr3_1:87414485-87414504 None:intergenic 10.0%
!! ATAATTATTAAAAATAGTAC+CGG - chr3_1:87415098-87415117 None:intergenic 10.0%
!! AATTACTAATATATACAATA+AGG - chr3_1:87416328-87416347 None:intergenic 10.0%
!!! AATTTTTTTTTGTCCTTTTT+TGG + chr3_1:87413019-87413038 Msa0384370:five_prime_UTR 15.0%
!!! TGTTTATTAAGTTTATTTTG+GGG + chr3_1:87414149-87414168 Msa0384370:intron 15.0%
!!! ATATGCATTTTTATTTTTCA+TGG + chr3_1:87414306-87414325 Msa0384370:intron 15.0%
!! TTATTAATAATAATTCTGAC+TGG + chr3_1:87414686-87414705 Msa0384370:intron 15.0%
!!! TAGTTTATTAATTGTTGTTT+TGG + chr3_1:87414932-87414951 Msa0384370:intron 15.0%
!! TGGTATATATAGTAAAAAAA+AGG - chr3_1:87415028-87415047 None:intergenic 15.0%
!! TATCAGATAATTTACAAATT+CGG - chr3_1:87415804-87415823 None:intergenic 15.0%
!! TTACTATGTTACATATTAAT+CGG + chr3_1:87416106-87416125 Msa0384370:three_prime_UTR 15.0%
!!! TTTTTTTTTCTTTTTCTTCG+TGG - chr3_1:87413054-87413073 None:intergenic 20.0%
!!! CATTTCATTTCTAATTTTCT+GGG - chr3_1:87413092-87413111 None:intergenic 20.0%
!!! TCATTTCATTTCTAATTTTC+TGG - chr3_1:87413093-87413112 None:intergenic 20.0%
!! TGAAATTGAAATGATAAGAA+TGG - chr3_1:87413739-87413758 None:intergenic 20.0%
!!! TAACCGTTTTTTTTTTCTTT+CGG + chr3_1:87413864-87413883 Msa0384370:intron 20.0%
!! AAATCAAAACTTGAATGATT+TGG - chr3_1:87413938-87413957 None:intergenic 20.0%
!!! AATTTTGATTCTCAAAATGT+TGG + chr3_1:87413965-87413984 Msa0384370:intron 20.0%
!!! ATTTTGATTCTCAAAATGTT+GGG + chr3_1:87413966-87413985 Msa0384370:intron 20.0%
!! ATATTGGAAAATAATACCAA+AGG - chr3_1:87414216-87414235 None:intergenic 20.0%
!! GTTAATCATTATTATTGAGA+TGG - chr3_1:87414765-87414784 None:intergenic 20.0%
!!! TCCTATAATAACTAATCTTT+TGG + chr3_1:87415475-87415494 Msa0384370:intron 20.0%
!!! ACCAAAAGATTAGTTATTAT+AGG - chr3_1:87415479-87415498 None:intergenic 20.0%
!!! CTTAGTACTTACTTTTAAAA+TGG + chr3_1:87415549-87415568 Msa0384370:intron 20.0%
!!! AAGATAGAAACTTGATAATA+TGG - chr3_1:87415767-87415786 None:intergenic 20.0%
!! TACTATGTTACATATTAATC+GGG + chr3_1:87416107-87416126 Msa0384370:three_prime_UTR 20.0%
!!! TTAATAGTTTAAGGTTATAG+AGG + chr3_1:87416159-87416178 Msa0384370:three_prime_UTR 20.0%
!! CATATATCTACATAATAACT+GGG - chr3_1:87416208-87416227 None:intergenic 20.0%
!! ACATATATCTACATAATAAC+TGG - chr3_1:87416209-87416228 None:intergenic 20.0%
!! AAAAACTTCAAATCATAGAA+CGG - chr3_1:87416466-87416485 None:intergenic 20.0%
!!! TTGTTGTTATCAAAAGTATT+AGG + chr3_1:87416492-87416511 Msa0384370:three_prime_UTR 20.0%
! AAACACATATATTTGTTTGC+TGG - chr3_1:87412985-87413004 None:intergenic 25.0%
!!! AACCGTTTTTTTTTTCTTTC+GGG + chr3_1:87413865-87413884 Msa0384370:intron 25.0%
! AACCCGAAAGAAAAAAAAAA+CGG - chr3_1:87413870-87413889 None:intergenic 25.0%
! GGATAAGAATTCATAAAGTA+GGG - chr3_1:87413917-87413936 None:intergenic 25.0%
! TGGATAAGAATTCATAAAGT+AGG - chr3_1:87413918-87413937 None:intergenic 25.0%
!!! TTTTGATTCTCAAAATGTTG+GGG + chr3_1:87413967-87413986 Msa0384370:intron 25.0%
!!! ATTTTTTGCTGTTGTTTATG+TGG + chr3_1:87414017-87414036 Msa0384370:intron 25.0%
!!! TTTTTTGCTGTTGTTTATGT+GGG + chr3_1:87414018-87414037 Msa0384370:intron 25.0%
!! ATTGGATTTTCTGAATCTAA+TGG + chr3_1:87414251-87414270 Msa0384370:intron 25.0%
!! TTGGATTTTCTGAATCTAAT+GGG + chr3_1:87414252-87414271 Msa0384370:intron 25.0%
!! AATCTAATGGGGATTTTATT+TGG + chr3_1:87414264-87414283 Msa0384370:intron 25.0%
!!! ATCTAATGGGGATTTTATTT+GGG + chr3_1:87414265-87414284 Msa0384370:intron 25.0%
! TGCAATTAACTAATTATCAG+TGG + chr3_1:87414365-87414384 Msa0384370:intron 25.0%
! TTTAGCTGTATAAATAATGC+AGG + chr3_1:87414505-87414524 Msa0384370:intron 25.0%
!! CTGTTCTGTTTTATCTTAAA+GGG + chr3_1:87414714-87414733 Msa0384370:intron 25.0%
! TCATTATTATTGAGATGGAA+TGG - chr3_1:87414760-87414779 None:intergenic 25.0%
!! CTTAATTGTTTTCTCTTTAG+GGG - chr3_1:87414804-87414823 None:intergenic 25.0%
!! GCTTAATTGTTTTCTCTTTA+GGG - chr3_1:87414805-87414824 None:intergenic 25.0%
!! AGCTTAATTGTTTTCTCTTT+AGG - chr3_1:87414806-87414825 None:intergenic 25.0%
!!! TATTAAAAATAGTACCGGTA+GGG - chr3_1:87415093-87415112 None:intergenic 25.0%
!!! TTATTAAAAATAGTACCGGT+AGG - chr3_1:87415094-87415113 None:intergenic 25.0%
! TATCTTATTGAAGATGCTTA+TGG + chr3_1:87415504-87415523 Msa0384370:intron 25.0%
!!! TACTTTTAAAATGGATTTGC+AGG + chr3_1:87415558-87415577 Msa0384370:intron 25.0%
!! ATAATTTTTATGTACCAGCT+GGG - chr3_1:87415647-87415666 None:intergenic 25.0%
!! AATAATTTTTATGTACCAGC+TGG - chr3_1:87415648-87415667 None:intergenic 25.0%
!! ACAAGAGTTCTTTATTTCAA+TGG - chr3_1:87415701-87415720 None:intergenic 25.0%
! AATTTGAACTGTTGTAATTG+AGG + chr3_1:87415723-87415742 Msa0384370:intron 25.0%
!! AAGTTCTACAACAAACTTTT+AGG - chr3_1:87415855-87415874 None:intergenic 25.0%
!! CATATTTCTGATATAGTAGT+AGG + chr3_1:87416136-87416155 Msa0384370:three_prime_UTR 25.0%
! AGTAGTAGGTTAATAGTTTA+AGG + chr3_1:87416150-87416169 Msa0384370:three_prime_UTR 25.0%
! CAACAAGAACAGATTTTGTA+CGG - chr3_1:87413594-87413613 None:intergenic 30.0%
! TTCAAACCATTTTCTTGTGT+CGG + chr3_1:87413755-87413774 Msa0384370:intron 30.0%
TGCATGTTTCTCTTTATTAC+TGG + chr3_1:87414082-87414101 Msa0384370:intron 30.0%
TTTCTCTTTATTACTGGATC+TGG + chr3_1:87414088-87414107 Msa0384370:intron 30.0%
AAAATAATACCAAAGGAGGT+AGG - chr3_1:87414209-87414228 None:intergenic 30.0%
TTGGAAAATAATACCAAAGG+AGG - chr3_1:87414213-87414232 None:intergenic 30.0%
! TGGATTTTCTGAATCTAATG+GGG + chr3_1:87414253-87414272 Msa0384370:intron 30.0%
ATTGAAATCAAGTTTGTCGT+AGG + chr3_1:87414389-87414408 Msa0384370:intron 30.0%
ATAATGCAGGTTCTTACTAA+TGG + chr3_1:87414518-87414537 Msa0384370:intron 30.0%
ATTGCACGTTATCAAATTCA+AGG - chr3_1:87414616-87414635 None:intergenic 30.0%
!! TTGAATTTGATAACGTGCAA+TGG + chr3_1:87414615-87414634 Msa0384370:CDS 30.0%
! GCTGTTCTGTTTTATCTTAA+AGG + chr3_1:87414713-87414732 Msa0384370:intron 30.0%
GGTTGAATCACATTAATTCT+AGG - chr3_1:87414845-87414864 None:intergenic 30.0%
TACTCGGAATTACATAAACA+CGG - chr3_1:87414866-87414885 None:intergenic 30.0%
! TATTTATTTGTTGACCCTAC+CGG + chr3_1:87415076-87415095 Msa0384370:intron 30.0%
! GATTTAAAGCTGATATACCA+TGG - chr3_1:87415289-87415308 None:intergenic 30.0%
AAAATTATCTAATGCGTCAG+TGG - chr3_1:87415334-87415353 None:intergenic 30.0%
! CTTTGTATATTTTCTGCAGA+AGG + chr3_1:87415387-87415406 Msa0384370:intron 30.0%
!!! TTTTAAAATGGATTTGCAGG+TGG + chr3_1:87415561-87415580 Msa0384370:intron 30.0%
CAAGAAAGAAACACAATTTC+AGG - chr3_1:87415916-87415935 None:intergenic 30.0%
TTTCTTTCTTGTGAAGCAAA+TGG + chr3_1:87415925-87415944 Msa0384370:intron 30.0%
TGTAACATAGTAAGTACGAA+GGG - chr3_1:87416099-87416118 None:intergenic 30.0%
ATGTAACATAGTAAGTACGA+AGG - chr3_1:87416100-87416119 None:intergenic 30.0%
! AGGTACTAATTACTCTTTTG+TGG + chr3_1:87416179-87416198 Msa0384370:three_prime_UTR 30.0%
ATATCTACATAATAACTGGG+TGG - chr3_1:87416205-87416224 None:intergenic 30.0%
AATGAGTGACACGTAAGATT+GGG - chr3_1:87413178-87413197 None:intergenic 35.0%
!! AAAAGATAGCGGAGAAAATG+GGG - chr3_1:87413343-87413362 None:intergenic 35.0%
!! GAAAAGATAGCGGAGAAAAT+GGG - chr3_1:87413344-87413363 None:intergenic 35.0%
!! TGAAAAGATAGCGGAGAAAA+TGG - chr3_1:87413345-87413364 None:intergenic 35.0%
GAGTAAAGGTGAAAAGATAG+CGG - chr3_1:87413354-87413373 None:intergenic 35.0%
TGTGCGAATTTCATGAGTAA+AGG - chr3_1:87413368-87413387 None:intergenic 35.0%
AGACGAAATGATTGAAGCAA+TGG + chr3_1:87413441-87413460 Msa0384370:exon 35.0%
GCAATGGAAAGTTCAGTTAA+CGG + chr3_1:87413457-87413476 Msa0384370:CDS 35.0%
! CATACTAAGGTTGTTGTTAC+TGG + chr3_1:87413562-87413581 Msa0384370:CDS 35.0%
CGTACAAAATCTGTTCTTGT+TGG + chr3_1:87413592-87413611 Msa0384370:CDS 35.0%
GTTAAGAAAGCTGTTGGATT+AGG + chr3_1:87413628-87413647 Msa0384370:CDS 35.0%
AATTGACCGACACAAGAAAA+TGG - chr3_1:87413764-87413783 None:intergenic 35.0%
!! TTTCTTGTGTCGGTCAATTT+TGG + chr3_1:87413765-87413784 Msa0384370:intron 35.0%
GAGAAACATGCAATGAATAC+AGG - chr3_1:87414074-87414093 None:intergenic 35.0%
AGTATATTACCTACCTCCTT+TGG + chr3_1:87414197-87414216 Msa0384370:intron 35.0%
GAATTGAAGTGAAGCTACAA+AGG + chr3_1:87414540-87414559 Msa0384370:CDS 35.0%
!! TTTGATAACGTGCAATGGAA+TGG + chr3_1:87414620-87414639 Msa0384370:CDS 35.0%
CAATGGAATGGATCAGATAT+GGG + chr3_1:87414632-87414651 Msa0384370:CDS 35.0%
CAGAAACAAGTACTAGTACT+CGG - chr3_1:87414882-87414901 None:intergenic 35.0%
GTTTGCAAAGTGATAGAGTT+TGG + chr3_1:87414970-87414989 Msa0384370:intron 35.0%
! GTGATAGAGTTTGGTAACAT+TGG + chr3_1:87414979-87414998 Msa0384370:intron 35.0%
GGAAAAAAAGACACGCATAA+AGG - chr3_1:87415007-87415026 None:intergenic 35.0%
! GCAAAACACTAGAGTCTTTT+TGG - chr3_1:87415048-87415067 None:intergenic 35.0%
CACAGAATGCATAGATCATT+GGG + chr3_1:87415186-87415205 Msa0384370:CDS 35.0%
TACAAATTCGGCGTAATTTG+TGG - chr3_1:87415792-87415811 None:intergenic 35.0%
CTGATATGCATGAATTGTGA+TGG + chr3_1:87415818-87415837 Msa0384370:intron 35.0%
ATTTGTCAATGCTGCAAAGA+GGG + chr3_1:87415970-87415989 Msa0384370:CDS 35.0%
GATATATGTGACCTTGAAAC+TGG + chr3_1:87416220-87416239 Msa0384370:three_prime_UTR 35.0%
TAACCACTTCATTAGCCATA+GGG - chr3_1:87416425-87416444 None:intergenic 35.0%
ATAACCACTTCATTAGCCAT+AGG - chr3_1:87416426-87416445 None:intergenic 35.0%
GAAGTGGTTATTAATCACCT+TGG + chr3_1:87416435-87416454 Msa0384370:three_prime_UTR 35.0%
GTGGCATTGTGATCCAAAAA+AGG - chr3_1:87413035-87413054 None:intergenic 40.0%
GAATGAGTGACACGTAAGAT+TGG - chr3_1:87413179-87413198 None:intergenic 40.0%
! CTTACGTGTCACTCATTCAT+TGG + chr3_1:87413181-87413200 Msa0384370:five_prime_UTR 40.0%
GGTAGTGTTTATTGGTGTGT+TGG - chr3_1:87413233-87413252 None:intergenic 40.0%
! GAGTGACTGGTAGTGTTTAT+TGG - chr3_1:87413241-87413260 None:intergenic 40.0%
AAGAAGAGAGAGAAAGTGAG+AGG - chr3_1:87413269-87413288 None:intergenic 40.0%
!! AAAGATAGCGGAGAAAATGG+GGG - chr3_1:87413342-87413361 None:intergenic 40.0%
ATGGAAAGTTCAGTTAACGG+TGG + chr3_1:87413460-87413479 Msa0384370:CDS 40.0%
! ACTCATTTGCAAAGCTTTGG+TGG + chr3_1:87413496-87413515 Msa0384370:CDS 40.0%
TGTGTTACCTCGTCATACTA+AGG + chr3_1:87413549-87413568 Msa0384370:CDS 40.0%
ACAACAACCTTAGTATGACG+AGG - chr3_1:87413559-87413578 None:intergenic 40.0%
!! TCTGTTCTTGTTGGTCTTCA+AGG + chr3_1:87413601-87413620 Msa0384370:CDS 40.0%
! AAGAAAGCTGTTGGATTAGG+TGG + chr3_1:87413631-87413650 Msa0384370:CDS 40.0%
TCATCTTCTTCATTGCCAGT+GGG - chr3_1:87414590-87414609 None:intergenic 40.0%
ATCATCTTCTTCATTGCCAG+TGG - chr3_1:87414591-87414610 None:intergenic 40.0%
GCAATGGAATGGATCAGATA+TGG + chr3_1:87414631-87414650 Msa0384370:CDS 40.0%
TCTATCACTTTGCAAACCCA+TGG - chr3_1:87414967-87414986 None:intergenic 40.0%
GCACAGAATGCATAGATCAT+TGG + chr3_1:87415185-87415204 Msa0384370:CDS 40.0%
TCTGGTGAATCACAGTCTAA+GGG + chr3_1:87415240-87415259 Msa0384370:CDS 40.0%
TATCTAATGCGTCAGTGGTA+GGG - chr3_1:87415329-87415348 None:intergenic 40.0%
TTATCTAATGCGTCAGTGGT+AGG - chr3_1:87415330-87415349 None:intergenic 40.0%
TGACTTGAGCAAGCTTGAAA+TGG + chr3_1:87415411-87415430 Msa0384370:CDS 40.0%
! AGCTAGTTGATGTTGTTCAG+AGG + chr3_1:87415611-87415630 Msa0384370:CDS 40.0%
GATTTGTCAATGCTGCAAAG+AGG + chr3_1:87415969-87415988 Msa0384370:CDS 40.0%
AGACTGTGTGCAAATGAGAT+TGG + chr3_1:87415996-87416015 Msa0384370:exon 40.0%
AACATAGTAAGTACGAAGGG+AGG - chr3_1:87416096-87416115 None:intergenic 40.0%
GAACATCGAAAAGAAAGCCT+TGG - chr3_1:87416261-87416280 None:intergenic 40.0%
ATCATAGAACGGTCTTACCA+AGG - chr3_1:87416455-87416474 None:intergenic 40.0%
ACACGTAAGATTGGGAAGGT+GGG - chr3_1:87413170-87413189 None:intergenic 45.0%
AGTGACACGTAAGATTGGGA+AGG - chr3_1:87413174-87413193 None:intergenic 45.0%
! ATTGGTTCATTCCCTCTCGT+CGG + chr3_1:87413199-87413218 Msa0384370:five_prime_UTR 45.0%
GGAGAAAATGGGGGAAATGT+AGG - chr3_1:87413333-87413352 None:intergenic 45.0%
GAAAGTTCAGTTAACGGTGG+TGG + chr3_1:87413463-87413482 Msa0384370:CDS 45.0%
GCGACTCATTTGCAAAGCTT+TGG + chr3_1:87413493-87413512 Msa0384370:CDS 45.0%
! CATTTGCAAAGCTTTGGTGG+TGG + chr3_1:87413499-87413518 Msa0384370:CDS 45.0%
! TGGTGGTGATAGTAGTGAAG+AGG + chr3_1:87413519-87413538 Msa0384370:CDS 45.0%
GGCGTTGTTAAGAAAGCTGT+TGG + chr3_1:87413622-87413641 Msa0384370:CDS 45.0%
! AAGCTGTTGGATTAGGTGGT+TGG + chr3_1:87413635-87413654 Msa0384370:CDS 45.0%
! TTGGATTAGGTGGTTGGCAT+TGG + chr3_1:87413641-87413660 Msa0384370:CDS 45.0%
!!! TGTTTTGGCTTCCATGACCA+TGG + chr3_1:87414947-87414966 Msa0384370:intron 45.0%
!!! GTTTTGGCTTCCATGACCAT+GGG + chr3_1:87414948-87414967 Msa0384370:intron 45.0%
ACTTTGCAAACCCATGGTCA+TGG - chr3_1:87414961-87414980 None:intergenic 45.0%
CACACAAGTCTCACAAATCG+AGG + chr3_1:87415163-87415182 Msa0384370:CDS 45.0%
CATGTCACAAGACCATGAGT+AGG + chr3_1:87415211-87415230 Msa0384370:CDS 45.0%
AGACTGTGATTCACCAGAGA+AGG - chr3_1:87415238-87415257 None:intergenic 45.0%
CTCTGGTGAATCACAGTCTA+AGG + chr3_1:87415239-87415258 Msa0384370:CDS 45.0%
CTGGTGAATCACAGTCTAAG+GGG + chr3_1:87415241-87415260 Msa0384370:CDS 45.0%
TGATATACCATGGACCCATG+AGG - chr3_1:87415279-87415298 None:intergenic 45.0%
AGCTTGAAATGGCTGCATTG+TGG + chr3_1:87415422-87415441 Msa0384370:CDS 45.0%
TGGCTGCATTGTGGAGATAT+TGG + chr3_1:87415431-87415450 Msa0384370:CDS 45.0%
CAACTAGCTGCTCCTTTGAT+GGG - chr3_1:87415600-87415619 None:intergenic 45.0%
TCAACTAGCTGCTCCTTTGA+TGG - chr3_1:87415601-87415620 None:intergenic 45.0%
GACGAGTTGCAGGTCATTAA+GGG + chr3_1:87415947-87415966 Msa0384370:CDS 45.0%
TGACCTTGAAACTGGTCGTA+AGG + chr3_1:87416228-87416247 Msa0384370:three_prime_UTR 45.0%
GACCTTGAAACTGGTCGTAA+GGG + chr3_1:87416229-87416248 Msa0384370:three_prime_UTR 45.0%
TTCCCTTACGACCAGTTTCA+AGG - chr3_1:87416234-87416253 None:intergenic 45.0%
!! CATTAATAATTAAAATATTA+AGG - chr3_1:87415360-87415379 None:intergenic 5.0%
! GTTTGCTGGCAGAGTGAAGA+TGG - chr3_1:87412971-87412990 None:intergenic 50.0%
GAGAGAGACTAGAGAGAGAG+CGG - chr3_1:87413120-87413139 None:intergenic 50.0%
GACACGTAAGATTGGGAAGG+TGG - chr3_1:87413171-87413190 None:intergenic 50.0%
TTGGTTCATTCCCTCTCGTC+GGG + chr3_1:87413200-87413219 Msa0384370:five_prime_UTR 50.0%
TGGTATGAATCCCGACGAGA+GGG - chr3_1:87413213-87413232 None:intergenic 50.0%
TTGGTATGAATCCCGACGAG+AGG - chr3_1:87413214-87413233 None:intergenic 50.0%
! AGTTCAGTTAACGGTGGTGG+TGG + chr3_1:87413466-87413485 Msa0384370:CDS 50.0%
!! TTGCAAAGCTTTGGTGGTGG+TGG + chr3_1:87413502-87413521 Msa0384370:CDS 50.0%
!! ATTAGGTGGTTGGCATTGGC+TGG + chr3_1:87413645-87413664 Msa0384370:CDS 50.0%
ACCATGAGTAGGTCCTTCTC+TGG + chr3_1:87415222-87415241 Msa0384370:CDS 50.0%
ACCAGAGAAGGACCTACTCA+TGG - chr3_1:87415226-87415245 None:intergenic 50.0%
GGTCCGTTACTATGCCTCAT+GGG + chr3_1:87415262-87415281 Msa0384370:CDS 50.0%
GGACCCATGAGGCATAGTAA+CGG - chr3_1:87415268-87415287 None:intergenic 50.0%
TACTATGCCTCATGGGTCCA+TGG + chr3_1:87415269-87415288 Msa0384370:CDS 50.0%
TGTCGTCCCAAACCCATCAA+AGG + chr3_1:87415585-87415604 Msa0384370:CDS 50.0%
!! GCTGCTCCTTTGATGGGTTT+GGG - chr3_1:87415594-87415613 None:intergenic 50.0%
!! AGCTGCTCCTTTGATGGGTT+TGG - chr3_1:87415595-87415614 None:intergenic 50.0%
GAAGCAAATGGACGAGTTGC+AGG + chr3_1:87415937-87415956 Msa0384370:intron 50.0%
GGACGAGTTGCAGGTCATTA+AGG + chr3_1:87415946-87415965 Msa0384370:CDS 50.0%
TCGATGTATTGCTGACCTGC+TGG + chr3_1:87416284-87416303 Msa0384370:three_prime_UTR 50.0%
CGATGTATTGCTGACCTGCT+GGG + chr3_1:87416285-87416304 Msa0384370:three_prime_UTR 50.0%
CTAAAAGCTCACTTCCCAGC+AGG - chr3_1:87416302-87416321 None:intergenic 50.0%
CTTCATTAGCCATAGGGTGG+TGG - chr3_1:87416419-87416438 None:intergenic 50.0%
CCACTTCATTAGCCATAGGG+TGG - chr3_1:87416422-87416441 None:intergenic 50.0%
CCACCCTATGGCTAATGAAG+TGG + chr3_1:87416419-87416438 Msa0384370:three_prime_UTR 50.0%
GTGAGAGGAGTGAGAGTGAC+TGG - chr3_1:87413254-87413273 None:intergenic 55.0%
! AGCGTGATTGAAGCACCCAC+TGG + chr3_1:87414572-87414591 Msa0384370:CDS 55.0%
GGGTCCGTTACTATGCCTCA+TGG + chr3_1:87415261-87415280 Msa0384370:CDS 55.0%
! GAGGCATTTCATGTCCCAGC+TGG + chr3_1:87415630-87415649 Msa0384370:CDS 55.0%
GGTCGTAAGGGAAGTCTCCA+AGG + chr3_1:87416241-87416260 Msa0384370:three_prime_UTR 55.0%
! CATTAGCCATAGGGTGGTGG+CGG - chr3_1:87416416-87416435 None:intergenic 55.0%
GCCAATCCGCCACCACCCTA+TGG + chr3_1:87416407-87416426 Msa0384370:three_prime_UTR 65.0%
!! GCCATAGGGTGGTGGCGGAT+TGG - chr3_1:87416411-87416430 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 87412924 87416521 87412924 ID=Msa0384370;Name=Msa0384370
chr3_1 mRNA 87412924 87416521 87412924 ID=Msa0384370-mRNA-1;Parent=Msa0384370;Name=Msa0384370-mRNA-1;_AED=0.11;_eAED=0.11;_QI=524|1|1|1|1|1|6|509|230
chr3_1 exon 87412924 87413666 87412924 ID=Msa0384370-mRNA-1:exon:23395;Parent=Msa0384370-mRNA-1
chr3_1 exon 87414527 87414653 87414527 ID=Msa0384370-mRNA-1:exon:23396;Parent=Msa0384370-mRNA-1
chr3_1 exon 87415151 87415290 87415151 ID=Msa0384370-mRNA-1:exon:23397;Parent=Msa0384370-mRNA-1
chr3_1 exon 87415406 87415468 87415406 ID=Msa0384370-mRNA-1:exon:23398;Parent=Msa0384370-mRNA-1
chr3_1 exon 87415580 87415651 87415580 ID=Msa0384370-mRNA-1:exon:23399;Parent=Msa0384370-mRNA-1
chr3_1 exon 87415941 87416521 87415941 ID=Msa0384370-mRNA-1:exon:23400;Parent=Msa0384370-mRNA-1
chr3_1 five_prime_UTR 87412924 87413447 87412924 ID=Msa0384370-mRNA-1:five_prime_utr;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87413448 87413666 87413448 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87414527 87414653 87414527 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87415151 87415290 87415151 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87415406 87415468 87415406 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87415580 87415651 87415580 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 CDS 87415941 87416012 87415941 ID=Msa0384370-mRNA-1:cds;Parent=Msa0384370-mRNA-1
chr3_1 three_prime_UTR 87416013 87416521 87416013 ID=Msa0384370-mRNA-1:three_prime_utr;Parent=Msa0384370-mRNA-1
Gene Sequence

>Msa0384370

ATGATTGAAGCAATGGAAAGTTCAGTTAACGGTGGTGGTGGTTTCGCGACTCATTTGCAAAGCTTTGGTGGTGGTGGTGATAGTAGTGAAGAGGAACTGTCTGTGTTACCTCGTCATACTAAGGTTGTTGTTACTGGAAATAACCGTACAAAATCTGTTCTTGTTGGTCTTCAAGGCGTTGTTAAGAAAGCTGTTGGATTAGGTGGTTGGCATTGGCTGGTTCTTACTAATGGAATTGAAGTGAAGCTACAAAGGAATGCTCTTAGCGTGATTGAAGCACCCACTGGCAATGAAGAAGATGATGACCTTGAATTTGATAACGTGCAATGGAATGGATCAGATATGGCATCAGACGATACACACAAGTCTCACAAATCGAGGCACAGAATGCATAGATCATTGGGATCATGTCACAAGACCATGAGTAGGTCCTTCTCTGGTGAATCACAGTCTAAGGGGTCCGTTACTATGCCTCATGGGTCCATGAAGGTTGACTTGAGCAAGCTTGAAATGGCTGCATTGTGGAGATATTGGAGACATTTTAATCTTGTGGATGTCGTCCCAAACCCATCAAAGGAGCAGCTAGTTGATGTTGTTCAGAGGCATTTCATGTCCCAGCTGCAAATGGACGAGTTGCAGGTCATTAAGGGATTTGTCAATGCTGCAAAGAGGGTCAAGACTGTGTGCAAATGA
Protein sequence

>Msa0384370

MIEAMESSVNGGGGFATHLQSFGGGGDSSEEELSVLPRHTKVVVTGNNRTKSVLVGLQGVVKKAVGLGGWHWLVLTNGIEVKLQRNALSVIEAPTGNEEDDDLEFDNVQWNGSDMASDDTHKSHKSRHRMHRSLGSCHKTMSRSFSGESQSKGSVTMPHGSMKVDLSKLEMAALWRYWRHFNLVDVVPNPSKEQLVDVVQRHFMSQLQMDELQVIKGFVNAAKRVKTVCK*