AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0522700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522700 MtrunA17_Chr3g0141391 98.701 231 2 1 1 230 1 231 2.14e-169 465
Msa0522700 MtrunA17_Chr1g0154621 83.550 231 32 4 1 230 1 226 1.33e-136 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0522700 AT1G19330.2 76.522 230 51 3 1 230 1 227 6.26e-111 318
Msa0522700 AT1G19330.1 75.319 235 50 4 1 230 1 232 3.24e-109 314
Msa0522700 AT1G19330.3 75.000 236 50 5 1 230 1 233 5.65e-108 311
Msa0522700 AT1G75060.1 69.787 235 64 5 1 230 1 233 2.50e-106 306
Msa0522700 AT1G75060.2 69.362 235 64 6 1 230 1 232 8.14e-104 300
Msa0522700 AT1G75060.3 70.370 189 50 4 1 184 1 188 1.03e-80 240

Find 118 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTAGTAGGTTAATAGTTTA+AGG 0.109374 3_4:-76012977 Msa0522700:three_prime_UTR
CATACTAAGGTTGTTGTTAC+TGG 0.194013 3_4:-76015571 Msa0522700:CDS
AGCTGCTCCTTTGATGGGTT+TGG 0.231841 3_4:+76013535 None:intergenic
CTCTGGTGATTCACAGTCTA+AGG 0.252293 3_4:-76013892 Msa0522700:CDS
GGACGAGTTGCAGGTCATTA+AGG 0.256826 3_4:-76013181 Msa0522700:CDS
TTACTATGTTACATATTAAT+CGG 0.270714 3_4:-76013021 Msa0522700:three_prime_UTR
ACATATATCTACATAATAAC+TGG 0.282748 3_4:+76012921 None:intergenic
TGAAAAGATAGCGGAGAAAA+TGG 0.292286 3_4:+76015791 None:intergenic
TTGTTGTTATCAAAAGTATT+AGG 0.312640 3_4:-76012635 Msa0522700:three_prime_UTR
TCTGTTCTTGTTGGTCTTCA+AGG 0.325218 3_4:-76015532 Msa0522700:CDS
GTTAAGAAAGCTGTTGGATT+AGG 0.325633 3_4:-76015505 Msa0522700:CDS
GCAATGGAATGGATCAGATA+TGG 0.328027 3_4:-76014500 Msa0522700:CDS
ATAATGCAGGTTCTTACTAA+TGG 0.333844 3_4:-76014613 Msa0522700:intron
GCTGCTCCTTTGATGGGTTT+GGG 0.336272 3_4:+76013536 None:intergenic
CATATTTCTGATATAGTAGT+AGG 0.336697 3_4:-76012991 Msa0522700:three_prime_UTR
TCAACTAGCTGCTCCTTTGA+TGG 0.362105 3_4:+76013529 None:intergenic
CTTACGTGTCACTCATTCAT+TGG 0.366812 3_4:-76015952 Msa0522700:five_prime_UTR
TGGCTGCATTGTGGAGATAT+TGG 0.369691 3_4:-76013700 Msa0522700:CDS
GAGTGACTGGTAGTGTTTAT+TGG 0.371943 3_4:+76015895 None:intergenic
GAAGCAAATGGACGAGTTGC+AGG 0.380501 3_4:-76013190 Msa0522700:intron
GACGAGTTGCAGGTCATTAA+GGG 0.386548 3_4:-76013180 Msa0522700:CDS
GCGACTCATTTGCAAAGCTT+TGG 0.398902 3_4:-76015640 Msa0522700:CDS
GAAAAGATAGCGGAGAAAAT+GGG 0.401309 3_4:+76015792 None:intergenic
AAACACATATATTTGTTTGC+TGG 0.402147 3_4:+76016150 None:intergenic
TTAATAGTTTAAGGTTATAG+AGG 0.402780 3_4:-76012968 Msa0522700:three_prime_UTR
CGTACAAAATCTGTTCTTGT+TGG 0.403520 3_4:-76015541 Msa0522700:CDS
ATTGCACGTTATCAAATTCA+AGG 0.405029 3_4:+76014518 None:intergenic
GAATGAGTGACACGTAAGAT+TGG 0.412038 3_4:+76015957 None:intergenic
GTGAGAGGAGTGAGAGTGAC+TGG 0.413347 3_4:+76015882 None:intergenic
TCGATGTATTGCTGACCTGC+TGG 0.417680 3_4:-76012843 Msa0522700:three_prime_UTR
TTGGATTAGGTGGTTGGCAT+TGG 0.430118 3_4:-76015492 Msa0522700:CDS
TACTATGTTACATATTAATC+GGG 0.431732 3_4:-76013020 Msa0522700:three_prime_UTR
TTCCCTTACGACCAGTTTCA+AGG 0.436157 3_4:+76012896 None:intergenic
CACAGAATGCATAGATCATT+GGG 0.437599 3_4:-76013945 Msa0522700:CDS
ATTAGGTGGTTGGCATTGGC+TGG 0.438971 3_4:-76015488 Msa0522700:intron
GCACAGAATGCATAGATCAT+TGG 0.439918 3_4:-76013946 Msa0522700:CDS
AATTACTAATATATACAATA+AGG 0.444214 3_4:+76012802 None:intergenic
GGCGTTGTTAAGAAAGCTGT+TGG 0.447387 3_4:-76015511 Msa0522700:CDS
TGACTTGAGCAAGCTTGAAA+TGG 0.450063 3_4:-76013720 Msa0522700:CDS
TTGCAAAGCTTTGGTGGTGG+TGG 0.460216 3_4:-76015631 Msa0522700:CDS
GATATATGTGACCTTGAAAC+TGG 0.460934 3_4:-76012907 Msa0522700:three_prime_UTR
GGTAGTGTTTATTGGTGTGT+TGG 0.462216 3_4:+76015903 None:intergenic
TTGGTTCATTCCCTCTCGTC+GGG 0.468051 3_4:-76015933 Msa0522700:five_prime_UTR
GTTTGCTGGCAGAGTGAAGA+TGG 0.478149 3_4:+76016164 None:intergenic
GCCAATCCGCCACCACCCTA+TGG 0.479576 3_4:-76012720 Msa0522700:three_prime_UTR
ACTCATTTGCAAAGCTTTGG+TGG 0.481146 3_4:-76015637 Msa0522700:CDS
CAACTAGCTGCTCCTTTGAT+GGG 0.486144 3_4:+76013530 None:intergenic
AATGAGTGACACGTAAGATT+GGG 0.487610 3_4:+76015958 None:intergenic
AGACTGTGTGCAAATGAGAT+TGG 0.492330 3_4:-76013131 Msa0522700:exon
TCTGGTGATTCACAGTCTAA+GGG 0.496708 3_4:-76013891 Msa0522700:CDS
AGTTCAGTTAACGGTGGTGG+TGG 0.498339 3_4:-76015667 Msa0522700:CDS
ATCATCTTCTTCATTGCCAG+TGG 0.499955 3_4:+76014543 None:intergenic
GGGTCCGTTACTATGCCTCA+TGG 0.500458 3_4:-76013870 Msa0522700:CDS
GCCATAGGGTGGTGGCGGAT+TGG 0.502890 3_4:+76012719 None:intergenic
AAGCTGTTGGATTAGGTGGT+TGG 0.508473 3_4:-76015498 Msa0522700:CDS
ATTGGTTCATTCCCTCTCGT+CGG 0.508541 3_4:-76015934 Msa0522700:five_prime_UTR
GCAATGGAAAGTTCAGTTAA+CGG 0.508721 3_4:-76015676 Msa0522700:CDS
ACCATGAGTAGGTCCTTCTC+TGG 0.512079 3_4:-76013909 Msa0522700:CDS
TGTGTTACCTCGTCATACTA+AGG 0.520326 3_4:-76015584 Msa0522700:CDS
TACTATGCCTCATGGGTCCA+TGG 0.522112 3_4:-76013862 Msa0522700:intron
TTGAATTTGATAACGTGCAA+TGG 0.522138 3_4:-76014516 Msa0522700:CDS
ATAACCACTTCATTAGCCAT+AGG 0.525655 3_4:+76012704 None:intergenic
TGACCTTGAAACTGGTCGTA+AGG 0.527053 3_4:-76012899 Msa0522700:three_prime_UTR
CATTTGCAAAGCTTTGGTGG+TGG 0.527791 3_4:-76015634 Msa0522700:CDS
AAAAGATAGCGGAGAAAATG+GGG 0.534955 3_4:+76015793 None:intergenic
AGACGAAATGATTGAAGCAA+TGG 0.538460 3_4:-76015692 Msa0522700:exon
GAGGCATTTCATGTCCCAGC+TGG 0.539302 3_4:-76013497 Msa0522700:intron
CAATGGAATGGATCAGATAT+GGG 0.541429 3_4:-76014499 Msa0522700:intron
CATTAGCCATAGGGTGGTGG+CGG 0.545825 3_4:+76012714 None:intergenic
CTAAAAGCTCACTTCCCAGC+AGG 0.547536 3_4:+76012828 None:intergenic
GAACATCGAAAAGAAAGCCT+TGG 0.547931 3_4:+76012869 None:intergenic
TGTGCGAATTTCATGAGTAA+AGG 0.552018 3_4:+76015768 None:intergenic
TAACCACTTCATTAGCCATA+GGG 0.553236 3_4:+76012705 None:intergenic
AAGAAAGCTGTTGGATTAGG+TGG 0.556533 3_4:-76015502 Msa0522700:CDS
ATTTGTCAATGCTGCAAAGA+GGG 0.558483 3_4:-76013157 Msa0522700:CDS
TTTGATAACGTGCAATGGAA+TGG 0.564949 3_4:-76014511 Msa0522700:CDS
CTTCATTAGCCATAGGGTGG+TGG 0.564958 3_4:+76012711 None:intergenic
GACACGTAAGATTGGGAAGG+TGG 0.565769 3_4:+76015965 None:intergenic
TCATCTTCTTCATTGCCAGT+GGG 0.573087 3_4:+76014544 None:intergenic
AGCGTGATTGAAGCACCCAC+TGG 0.574928 3_4:-76014559 Msa0522700:CDS
GACCTTGAAACTGGTCGTAA+GGG 0.580014 3_4:-76012898 Msa0522700:three_prime_UTR
GGTCGTAAGGGAAGTCTCCA+AGG 0.583097 3_4:-76012886 Msa0522700:three_prime_UTR
CCACCCTATGGCTAATGAAG+TGG 0.584364 3_4:-76012708 Msa0522700:three_prime_UTR
GAAAGTTCAGTTAACGGTGG+TGG 0.587165 3_4:-76015670 Msa0522700:CDS
AAGAAGAGAGAGAAAGTGAG+AGG 0.587528 3_4:+76015867 None:intergenic
CGATGTATTGCTGACCTGCT+GGG 0.594454 3_4:-76012842 Msa0522700:three_prime_UTR
TGTCGTCCCAAACCCATCAA+AGG 0.596940 3_4:-76013542 Msa0522700:CDS
GGTCCGTTACTATGCCTCAT+GGG 0.599829 3_4:-76013869 Msa0522700:CDS
CATATATCTACATAATAACT+GGG 0.605713 3_4:+76012922 None:intergenic
GAAGTGGTTATTAATCACCT+TGG 0.607458 3_4:-76012692 Msa0522700:three_prime_UTR
AGACTGTGAATCACCAGAGA+AGG 0.609518 3_4:+76013896 None:intergenic
ACCAGAGAAGGACCTACTCA+TGG 0.614361 3_4:+76013908 None:intergenic
GAATTGAAGTGAAGCTACAA+AGG 0.619531 3_4:-76014591 Msa0522700:CDS
CTGGTGATTCACAGTCTAAG+GGG 0.620120 3_4:-76013890 Msa0522700:CDS
AAACCAACCACGCAAACGCA+AGG 0.625045 3_4:-76016219 None:intergenic
TGGTATGAATCCCGACGAGA+GGG 0.629840 3_4:+76015923 None:intergenic
GAGAGAGACTAGAGAGAGAG+CGG 0.630294 3_4:+76016016 None:intergenic
TGTAACATAGTAAGTACGAA+GGG 0.633296 3_4:+76013031 None:intergenic
AGCTAGTTGATGTTGTTCAG+AGG 0.634780 3_4:-76013516 Msa0522700:CDS
ATCATAGAACGGTCTTACCA+AGG 0.638709 3_4:+76012675 None:intergenic
ACACGTAAGATTGGGAAGGT+GGG 0.639779 3_4:+76015966 None:intergenic
TTGGTATGAATCCCGACGAG+AGG 0.642355 3_4:+76015922 None:intergenic
AGCTTGAAATGGCTGCATTG+TGG 0.645923 3_4:-76013709 Msa0522700:CDS
TGGTGGTGATAGTAGTGAAG+AGG 0.648847 3_4:-76015614 Msa0522700:CDS
CACACAAGTCTCACAAATCG+AGG 0.652726 3_4:-76013968 Msa0522700:CDS
GATTTGTCAATGCTGCAAAG+AGG 0.660589 3_4:-76013158 Msa0522700:CDS
AAAGATAGCGGAGAAAATGG+GGG 0.664645 3_4:+76015794 None:intergenic
GAGTAAAGGTGAAAAGATAG+CGG 0.665485 3_4:+76015782 None:intergenic
ATGTAACATAGTAAGTACGA+AGG 0.675651 3_4:+76013030 None:intergenic
AGTGACACGTAAGATTGGGA+AGG 0.686314 3_4:+76015962 None:intergenic
GGACCCATGAGGCATAGTAA+CGG 0.688810 3_4:+76013866 None:intergenic
CCACTTCATTAGCCATAGGG+TGG 0.692185 3_4:+76012708 None:intergenic
AACATAGTAAGTACGAAGGG+AGG 0.698574 3_4:+76013034 None:intergenic
CATGTCACAAGACCATGAGT+AGG 0.700516 3_4:-76013920 Msa0522700:CDS
TGATATACCATGGACCCATG+AGG 0.707981 3_4:+76013855 None:intergenic
ATATCTACATAATAACTGGG+TGG 0.711276 3_4:+76012925 None:intergenic
ACAACAACCTTAGTATGACG+AGG 0.714849 3_4:+76015577 None:intergenic
ATGGAAAGTTCAGTTAACGG+TGG 0.729999 3_4:-76015673 Msa0522700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTCTATTTTTTATTATTA+AGG - chr3_4:76013394-76013413 Msa0522700:intron 10.0%
!!! ATTGTTTATTAAGTTTATTT+TGG - chr3_4:76013852-76013871 Msa0522700:intron 10.0%
!!! TTGTTTATTAAGTTTATTTT+GGG - chr3_4:76013853-76013872 Msa0522700:intron 10.0%
!! TAACACAATTTATTTAATAT+TGG + chr3_4:76013937-76013956 None:intergenic 10.0%
!! TATTAAATAAATTGTGTTAT+TGG - chr3_4:76013938-76013957 Msa0522700:CDS 10.0%
!!! AATTTAGATTATTTGAAATT+AGG - chr3_4:76014164-76014183 Msa0522700:intron 10.0%
!! ATTGTTAAAAAAAATCATTT+AGG + chr3_4:76014190-76014209 None:intergenic 10.0%
!! ATAATTATTAAAAATAGTAC+CGG + chr3_4:76014803-76014822 None:intergenic 10.0%
!! AATTACTAATATATACAATA+AGG + chr3_4:76016037-76016056 None:intergenic 10.0%
!!! AATTTTTTTTTGTCCTTTTT+TGG - chr3_4:76012723-76012742 Msa0522700:three_prime_UTR 15.0%
!!! TGTTTATTAAGTTTATTTTG+GGG - chr3_4:76013854-76013873 Msa0522700:intron 15.0%
!!! ATATGCATTTTTATTTTTCA+TGG - chr3_4:76014011-76014030 Msa0522700:intron 15.0%
!! TTATTAATAATAATTCTGAC+TGG - chr3_4:76014391-76014410 Msa0522700:intron 15.0%
!!! TAGTTTATTAATTGTTGTTT+TGG - chr3_4:76014637-76014656 Msa0522700:intron 15.0%
!! TATCAGATAATTTACAAATT+CGG + chr3_4:76015513-76015532 None:intergenic 15.0%
!! TTACTATGTTACATATTAAT+CGG - chr3_4:76015815-76015834 Msa0522700:five_prime_UTR 15.0%
!!! TTTTTTTTTCTTTTTCTTCG+TGG + chr3_4:76012758-76012777 None:intergenic 20.0%
!!! CATTTCATTTCTAATTTTCT+GGG + chr3_4:76012795-76012814 None:intergenic 20.0%
!!! TCATTTCATTTCTAATTTTC+TGG + chr3_4:76012796-76012815 None:intergenic 20.0%
!! TGAAATTGAAATGATAAGAA+TGG + chr3_4:76013442-76013461 None:intergenic 20.0%
!!! TAACCGTTTTTTTTTTCTTT+CGG - chr3_4:76013569-76013588 Msa0522700:intron 20.0%
!! AAATCAAAACTTGAATGATT+TGG + chr3_4:76013643-76013662 None:intergenic 20.0%
!!! AATTTTGATTCTCAAAATGT+TGG - chr3_4:76013670-76013689 Msa0522700:intron 20.0%
!!! ATTTTGATTCTCAAAATGTT+GGG - chr3_4:76013671-76013690 Msa0522700:intron 20.0%
!! ATATTGGAAAATAATACCAA+AGG + chr3_4:76013921-76013940 None:intergenic 20.0%
!! GTTAATCATTATTATTGAGA+TGG + chr3_4:76014470-76014489 None:intergenic 20.0%
!!! CTTAATTGTTTTTTCTTTAG+GGG + chr3_4:76014509-76014528 None:intergenic 20.0%
!!! GCTTAATTGTTTTTTCTTTA+GGG + chr3_4:76014510-76014529 None:intergenic 20.0%
!!! AGCTTAATTGTTTTTTCTTT+AGG + chr3_4:76014511-76014530 None:intergenic 20.0%
!! TGGTATATAGAGTAAAAAAA+AGG + chr3_4:76014733-76014752 None:intergenic 20.0%
!!! TCCTATAATAACTAATCTTT+TGG - chr3_4:76015180-76015199 Msa0522700:intron 20.0%
!!! ACCAAAAGATTAGTTATTAT+AGG + chr3_4:76015184-76015203 None:intergenic 20.0%
!!! AAGATAGAAACTTGATAATA+TGG + chr3_4:76015476-76015495 None:intergenic 20.0%
!! TACTATGTTACATATTAATC+GGG - chr3_4:76015816-76015835 Msa0522700:five_prime_UTR 20.0%
!!! TTAATAGTTTAAGGTTATAG+AGG - chr3_4:76015868-76015887 Msa0522700:five_prime_UTR 20.0%
!! CATATATCTACATAATAACT+GGG + chr3_4:76015917-76015936 None:intergenic 20.0%
!! ACATATATCTACATAATAAC+TGG + chr3_4:76015918-76015937 None:intergenic 20.0%
!! AAAAACTTCAAATCATAGAA+CGG + chr3_4:76016175-76016194 None:intergenic 20.0%
!!! TTGTTGTTATCAAAAGTATT+AGG - chr3_4:76016201-76016220 Msa0522700:five_prime_UTR 20.0%
! AAACACATATATTTGTTTGC+TGG + chr3_4:76012689-76012708 None:intergenic 25.0%
!!! AACCGTTTTTTTTTTCTTTC+GGG - chr3_4:76013570-76013589 Msa0522700:intron 25.0%
! AACCCGAAAGAAAAAAAAAA+CGG + chr3_4:76013575-76013594 None:intergenic 25.0%
! GGATAAGAATTCATAAAGTA+GGG + chr3_4:76013622-76013641 None:intergenic 25.0%
! TGGATAAGAATTCATAAAGT+AGG + chr3_4:76013623-76013642 None:intergenic 25.0%
!!! TTTTGATTCTCAAAATGTTG+GGG - chr3_4:76013672-76013691 Msa0522700:intron 25.0%
!!! ATTTTTTGCTGTTGTTTATG+TGG - chr3_4:76013722-76013741 Msa0522700:CDS 25.0%
!!! TTTTTTGCTGTTGTTTATGT+GGG - chr3_4:76013723-76013742 Msa0522700:CDS 25.0%
!! ATTGGATTTTCTGAATCTAA+TGG - chr3_4:76013956-76013975 Msa0522700:CDS 25.0%
!! TTGGATTTTCTGAATCTAAT+GGG - chr3_4:76013957-76013976 Msa0522700:CDS 25.0%
!! AATCTAATGGGGATTTTATT+TGG - chr3_4:76013969-76013988 Msa0522700:CDS 25.0%
!!! ATCTAATGGGGATTTTATTT+GGG - chr3_4:76013970-76013989 Msa0522700:CDS 25.0%
! TGCAATTAACTAATTATCAG+TGG - chr3_4:76014070-76014089 Msa0522700:intron 25.0%
! TTTAGCTGTATAAATAATGC+AGG - chr3_4:76014210-76014229 Msa0522700:intron 25.0%
!! CTGTTCTGTTTTATCTTAAA+GGG - chr3_4:76014419-76014438 Msa0522700:intron 25.0%
! TCATTATTATTGAGATGGAA+TGG + chr3_4:76014465-76014484 None:intergenic 25.0%
! AGTTGAATCACATTAATTCT+AGG + chr3_4:76014550-76014569 None:intergenic 25.0%
! TATTTATTTATTGACCCTAC+CGG - chr3_4:76014781-76014800 Msa0522700:intron 25.0%
!!! TATTAAAAATAGTACCGGTA+GGG + chr3_4:76014798-76014817 None:intergenic 25.0%
!!! TTATTAAAAATAGTACCGGT+AGG + chr3_4:76014799-76014818 None:intergenic 25.0%
! TATCTTATTGAAGATGCTTA+TGG - chr3_4:76015209-76015228 Msa0522700:intron 25.0%
!!! GTACGTACTTACTTTTAAAA+TGG - chr3_4:76015258-76015277 Msa0522700:intron 25.0%
!!! TACTTTTAAAATGGATTTGC+AGG - chr3_4:76015267-76015286 Msa0522700:intron 25.0%
!! ATAATTTTTATGTACCAGCT+GGG + chr3_4:76015356-76015375 None:intergenic 25.0%
!! AATAATTTTTATGTACCAGC+TGG + chr3_4:76015357-76015376 None:intergenic 25.0%
!! ACAAGAGTTCTTTATTTCAA+TGG + chr3_4:76015410-76015429 None:intergenic 25.0%
! AATTTGAACTGTTGTAATTG+AGG - chr3_4:76015432-76015451 Msa0522700:intron 25.0%
!! AAGTTCTACAACAAACTTTT+AGG + chr3_4:76015564-76015583 None:intergenic 25.0%
!! CATATTTCTGATATAGTAGT+AGG - chr3_4:76015845-76015864 Msa0522700:five_prime_UTR 25.0%
! AGTAGTAGGTTAATAGTTTA+AGG - chr3_4:76015859-76015878 Msa0522700:five_prime_UTR 25.0%
! CAACAAGAACAGATTTTGTA+CGG + chr3_4:76013297-76013316 None:intergenic 30.0%
! TTCAAACCATTTTCTTGTGT+CGG - chr3_4:76013458-76013477 Msa0522700:intron 30.0%
TGCATGTTTCTCTTTATTAC+TGG - chr3_4:76013787-76013806 Msa0522700:intron 30.0%
TTTCTCTTTATTACTGGATC+TGG - chr3_4:76013793-76013812 Msa0522700:intron 30.0%
AAAATAATACCAAAGGAGGT+AGG + chr3_4:76013914-76013933 None:intergenic 30.0%
TTGGAAAATAATACCAAAGG+AGG + chr3_4:76013918-76013937 None:intergenic 30.0%
! TGGATTTTCTGAATCTAATG+GGG - chr3_4:76013958-76013977 Msa0522700:CDS 30.0%
ATTGAAATCAAGTTTGTCGT+AGG - chr3_4:76014094-76014113 Msa0522700:intron 30.0%
ATAATGCAGGTTCTTACTAA+TGG - chr3_4:76014223-76014242 Msa0522700:intron 30.0%
ATTGCACGTTATCAAATTCA+AGG + chr3_4:76014321-76014340 None:intergenic 30.0%
!! TTGAATTTGATAACGTGCAA+TGG - chr3_4:76014320-76014339 Msa0522700:intron 30.0%
! GCTGTTCTGTTTTATCTTAA+AGG - chr3_4:76014418-76014437 Msa0522700:intron 30.0%
! GATTTAAAGCTGATATACCA+TGG + chr3_4:76014994-76015013 None:intergenic 30.0%
AAAATTATCTAATGCGTCAG+TGG + chr3_4:76015039-76015058 None:intergenic 30.0%
! CTTTGTATATTTTCTGCAGA+AGG - chr3_4:76015092-76015111 Msa0522700:intron 30.0%
!!! TTTTAAAATGGATTTGCAGG+TGG - chr3_4:76015270-76015289 Msa0522700:intron 30.0%
CAAGAAAGAAACACAATTTC+AGG + chr3_4:76015625-76015644 None:intergenic 30.0%
TTTCTTTCTTGTGAAGCAAA+TGG - chr3_4:76015634-76015653 Msa0522700:CDS 30.0%
TGTAACATAGTAAGTACGAA+GGG + chr3_4:76015808-76015827 None:intergenic 30.0%
ATGTAACATAGTAAGTACGA+AGG + chr3_4:76015809-76015828 None:intergenic 30.0%
! AGGTACTAATTACTCTTTTG+TGG - chr3_4:76015888-76015907 Msa0522700:five_prime_UTR 30.0%
ATATCTACATAATAACTGGG+TGG + chr3_4:76015914-76015933 None:intergenic 30.0%
AATGAGTGACACGTAAGATT+GGG + chr3_4:76012881-76012900 None:intergenic 35.0%
!! AAAAGATAGCGGAGAAAATG+GGG + chr3_4:76013046-76013065 None:intergenic 35.0%
!! GAAAAGATAGCGGAGAAAAT+GGG + chr3_4:76013047-76013066 None:intergenic 35.0%
!! TGAAAAGATAGCGGAGAAAA+TGG + chr3_4:76013048-76013067 None:intergenic 35.0%
GAGTAAAGGTGAAAAGATAG+CGG + chr3_4:76013057-76013076 None:intergenic 35.0%
TGTGCGAATTTCATGAGTAA+AGG + chr3_4:76013071-76013090 None:intergenic 35.0%
AGACGAAATGATTGAAGCAA+TGG - chr3_4:76013144-76013163 Msa0522700:CDS 35.0%
GCAATGGAAAGTTCAGTTAA+CGG - chr3_4:76013160-76013179 Msa0522700:CDS 35.0%
! CATACTAAGGTTGTTGTTAC+TGG - chr3_4:76013265-76013284 Msa0522700:intron 35.0%
CGTACAAAATCTGTTCTTGT+TGG - chr3_4:76013295-76013314 Msa0522700:intron 35.0%
GTTAAGAAAGCTGTTGGATT+AGG - chr3_4:76013331-76013350 Msa0522700:intron 35.0%
AATTGACCGACACAAGAAAA+TGG + chr3_4:76013467-76013486 None:intergenic 35.0%
!! TTTCTTGTGTCGGTCAATTT+TGG - chr3_4:76013468-76013487 Msa0522700:intron 35.0%
GAGAAACATGCAATGAATAC+AGG + chr3_4:76013779-76013798 None:intergenic 35.0%
AGTATATTACCTACCTCCTT+TGG - chr3_4:76013902-76013921 Msa0522700:CDS 35.0%
GAATTGAAGTGAAGCTACAA+AGG - chr3_4:76014245-76014264 Msa0522700:intron 35.0%
!! TTTGATAACGTGCAATGGAA+TGG - chr3_4:76014325-76014344 Msa0522700:intron 35.0%
CAATGGAATGGATCAGATAT+GGG - chr3_4:76014337-76014356 Msa0522700:intron 35.0%
CAGAAACAAGTACTAGTACT+CGG + chr3_4:76014587-76014606 None:intergenic 35.0%
GTTTGCAAAGTGATAGAGTT+TGG - chr3_4:76014675-76014694 Msa0522700:intron 35.0%
! GTGATAGAGTTTGGTAACAT+TGG - chr3_4:76014684-76014703 Msa0522700:intron 35.0%
GGAAAAAAAGACACGCATAA+AGG + chr3_4:76014712-76014731 None:intergenic 35.0%
! GCAAAACACTAGAGTCTTTT+TGG + chr3_4:76014753-76014772 None:intergenic 35.0%
CACAGAATGCATAGATCATT+GGG - chr3_4:76014891-76014910 Msa0522700:intron 35.0%
TACAAATTCGGCGTAATTTG+TGG + chr3_4:76015501-76015520 None:intergenic 35.0%
CTGATATGCATGAATTGTGA+TGG - chr3_4:76015527-76015546 Msa0522700:CDS 35.0%
ATTTGTCAATGCTGCAAAGA+GGG - chr3_4:76015679-76015698 Msa0522700:CDS 35.0%
GATATATGTGACCTTGAAAC+TGG - chr3_4:76015929-76015948 Msa0522700:five_prime_UTR 35.0%
TAACCACTTCATTAGCCATA+GGG + chr3_4:76016134-76016153 None:intergenic 35.0%
ATAACCACTTCATTAGCCAT+AGG + chr3_4:76016135-76016154 None:intergenic 35.0%
GAAGTGGTTATTAATCACCT+TGG - chr3_4:76016144-76016163 Msa0522700:five_prime_UTR 35.0%
GTGGCATTGTGATCCAAAAA+AGG + chr3_4:76012739-76012758 None:intergenic 40.0%
GAATGAGTGACACGTAAGAT+TGG + chr3_4:76012882-76012901 None:intergenic 40.0%
! CTTACGTGTCACTCATTCAT+TGG - chr3_4:76012884-76012903 Msa0522700:three_prime_UTR 40.0%
GGTAGTGTTTATTGGTGTGT+TGG + chr3_4:76012936-76012955 None:intergenic 40.0%
! GAGTGACTGGTAGTGTTTAT+TGG + chr3_4:76012944-76012963 None:intergenic 40.0%
AAGAAGAGAGAGAAAGTGAG+AGG + chr3_4:76012972-76012991 None:intergenic 40.0%
!! AAAGATAGCGGAGAAAATGG+GGG + chr3_4:76013045-76013064 None:intergenic 40.0%
ATGGAAAGTTCAGTTAACGG+TGG - chr3_4:76013163-76013182 Msa0522700:CDS 40.0%
! ACTCATTTGCAAAGCTTTGG+TGG - chr3_4:76013199-76013218 Msa0522700:intron 40.0%
TGTGTTACCTCGTCATACTA+AGG - chr3_4:76013252-76013271 Msa0522700:intron 40.0%
ACAACAACCTTAGTATGACG+AGG + chr3_4:76013262-76013281 None:intergenic 40.0%
!! TCTGTTCTTGTTGGTCTTCA+AGG - chr3_4:76013304-76013323 Msa0522700:intron 40.0%
! AAGAAAGCTGTTGGATTAGG+TGG - chr3_4:76013334-76013353 Msa0522700:intron 40.0%
TCATCTTCTTCATTGCCAGT+GGG + chr3_4:76014295-76014314 None:intergenic 40.0%
ATCATCTTCTTCATTGCCAG+TGG + chr3_4:76014296-76014315 None:intergenic 40.0%
GCAATGGAATGGATCAGATA+TGG - chr3_4:76014336-76014355 Msa0522700:intron 40.0%
TCTATCACTTTGCAAACCCA+TGG + chr3_4:76014672-76014691 None:intergenic 40.0%
GCACAGAATGCATAGATCAT+TGG - chr3_4:76014890-76014909 Msa0522700:intron 40.0%
TCTGGTGATTCACAGTCTAA+GGG - chr3_4:76014945-76014964 Msa0522700:intron 40.0%
TATCTAATGCGTCAGTGGTA+GGG + chr3_4:76015034-76015053 None:intergenic 40.0%
TTATCTAATGCGTCAGTGGT+AGG + chr3_4:76015035-76015054 None:intergenic 40.0%
TGACTTGAGCAAGCTTGAAA+TGG - chr3_4:76015116-76015135 Msa0522700:intron 40.0%
! AGCTAGTTGATGTTGTTCAG+AGG - chr3_4:76015320-76015339 Msa0522700:intron 40.0%
GATTTGTCAATGCTGCAAAG+AGG - chr3_4:76015678-76015697 Msa0522700:CDS 40.0%
AGACTGTGTGCAAATGAGAT+TGG - chr3_4:76015705-76015724 Msa0522700:exon 40.0%
AACATAGTAAGTACGAAGGG+AGG + chr3_4:76015805-76015824 None:intergenic 40.0%
GAACATCGAAAAGAAAGCCT+TGG + chr3_4:76015970-76015989 None:intergenic 40.0%
ATCATAGAACGGTCTTACCA+AGG + chr3_4:76016164-76016183 None:intergenic 40.0%
ACACGTAAGATTGGGAAGGT+GGG + chr3_4:76012873-76012892 None:intergenic 45.0%
AGTGACACGTAAGATTGGGA+AGG + chr3_4:76012877-76012896 None:intergenic 45.0%
! ATTGGTTCATTCCCTCTCGT+CGG - chr3_4:76012902-76012921 Msa0522700:three_prime_UTR 45.0%
GGAGAAAATGGGGGAAATGT+AGG + chr3_4:76013036-76013055 None:intergenic 45.0%
GAAAGTTCAGTTAACGGTGG+TGG - chr3_4:76013166-76013185 Msa0522700:CDS 45.0%
GCGACTCATTTGCAAAGCTT+TGG - chr3_4:76013196-76013215 Msa0522700:intron 45.0%
! CATTTGCAAAGCTTTGGTGG+TGG - chr3_4:76013202-76013221 Msa0522700:intron 45.0%
! TGGTGGTGATAGTAGTGAAG+AGG - chr3_4:76013222-76013241 Msa0522700:intron 45.0%
GGCGTTGTTAAGAAAGCTGT+TGG - chr3_4:76013325-76013344 Msa0522700:intron 45.0%
! AAGCTGTTGGATTAGGTGGT+TGG - chr3_4:76013338-76013357 Msa0522700:intron 45.0%
! TTGGATTAGGTGGTTGGCAT+TGG - chr3_4:76013344-76013363 Msa0522700:intron 45.0%
!!! TGTTTTGGCTTCCATGACCA+TGG - chr3_4:76014652-76014671 Msa0522700:intron 45.0%
!!! GTTTTGGCTTCCATGACCAT+GGG - chr3_4:76014653-76014672 Msa0522700:intron 45.0%
ACTTTGCAAACCCATGGTCA+TGG + chr3_4:76014666-76014685 None:intergenic 45.0%
CACACAAGTCTCACAAATCG+AGG - chr3_4:76014868-76014887 Msa0522700:intron 45.0%
CATGTCACAAGACCATGAGT+AGG - chr3_4:76014916-76014935 Msa0522700:intron 45.0%
AGACTGTGAATCACCAGAGA+AGG + chr3_4:76014943-76014962 None:intergenic 45.0%
CTCTGGTGATTCACAGTCTA+AGG - chr3_4:76014944-76014963 Msa0522700:intron 45.0%
CTGGTGATTCACAGTCTAAG+GGG - chr3_4:76014946-76014965 Msa0522700:intron 45.0%
TGATATACCATGGACCCATG+AGG + chr3_4:76014984-76015003 None:intergenic 45.0%
AGCTTGAAATGGCTGCATTG+TGG - chr3_4:76015127-76015146 Msa0522700:intron 45.0%
TGGCTGCATTGTGGAGATAT+TGG - chr3_4:76015136-76015155 Msa0522700:intron 45.0%
CAACTAGCTGCTCCTTTGAT+GGG + chr3_4:76015309-76015328 None:intergenic 45.0%
TCAACTAGCTGCTCCTTTGA+TGG + chr3_4:76015310-76015329 None:intergenic 45.0%
GACGAGTTGCAGGTCATTAA+GGG - chr3_4:76015656-76015675 Msa0522700:CDS 45.0%
TGACCTTGAAACTGGTCGTA+AGG - chr3_4:76015937-76015956 Msa0522700:five_prime_UTR 45.0%
GACCTTGAAACTGGTCGTAA+GGG - chr3_4:76015938-76015957 Msa0522700:five_prime_UTR 45.0%
TTCCCTTACGACCAGTTTCA+AGG + chr3_4:76015943-76015962 None:intergenic 45.0%
!! CATTAATAATTAAAATATTA+AGG + chr3_4:76015065-76015084 None:intergenic 5.0%
! GTTTGCTGGCAGAGTGAAGA+TGG + chr3_4:76012675-76012694 None:intergenic 50.0%
GAGAGAGACTAGAGAGAGAG+CGG + chr3_4:76012823-76012842 None:intergenic 50.0%
GACACGTAAGATTGGGAAGG+TGG + chr3_4:76012874-76012893 None:intergenic 50.0%
TTGGTTCATTCCCTCTCGTC+GGG - chr3_4:76012903-76012922 Msa0522700:three_prime_UTR 50.0%
TGGTATGAATCCCGACGAGA+GGG + chr3_4:76012916-76012935 None:intergenic 50.0%
TTGGTATGAATCCCGACGAG+AGG + chr3_4:76012917-76012936 None:intergenic 50.0%
! AGTTCAGTTAACGGTGGTGG+TGG - chr3_4:76013169-76013188 Msa0522700:CDS 50.0%
!! TTGCAAAGCTTTGGTGGTGG+TGG - chr3_4:76013205-76013224 Msa0522700:intron 50.0%
!! ATTAGGTGGTTGGCATTGGC+TGG - chr3_4:76013348-76013367 Msa0522700:intron 50.0%
ACCATGAGTAGGTCCTTCTC+TGG - chr3_4:76014927-76014946 Msa0522700:intron 50.0%
ACCAGAGAAGGACCTACTCA+TGG + chr3_4:76014931-76014950 None:intergenic 50.0%
GGTCCGTTACTATGCCTCAT+GGG - chr3_4:76014967-76014986 Msa0522700:intron 50.0%
GGACCCATGAGGCATAGTAA+CGG + chr3_4:76014973-76014992 None:intergenic 50.0%
TACTATGCCTCATGGGTCCA+TGG - chr3_4:76014974-76014993 Msa0522700:intron 50.0%
TGTCGTCCCAAACCCATCAA+AGG - chr3_4:76015294-76015313 Msa0522700:intron 50.0%
!! GCTGCTCCTTTGATGGGTTT+GGG + chr3_4:76015303-76015322 None:intergenic 50.0%
!! AGCTGCTCCTTTGATGGGTT+TGG + chr3_4:76015304-76015323 None:intergenic 50.0%
GAAGCAAATGGACGAGTTGC+AGG - chr3_4:76015646-76015665 Msa0522700:CDS 50.0%
GGACGAGTTGCAGGTCATTA+AGG - chr3_4:76015655-76015674 Msa0522700:CDS 50.0%
TCGATGTATTGCTGACCTGC+TGG - chr3_4:76015993-76016012 Msa0522700:five_prime_UTR 50.0%
CGATGTATTGCTGACCTGCT+GGG - chr3_4:76015994-76016013 Msa0522700:five_prime_UTR 50.0%
CTAAAAGCTCACTTCCCAGC+AGG + chr3_4:76016011-76016030 None:intergenic 50.0%
CTTCATTAGCCATAGGGTGG+TGG + chr3_4:76016128-76016147 None:intergenic 50.0%
CCACTTCATTAGCCATAGGG+TGG + chr3_4:76016131-76016150 None:intergenic 50.0%
CCACCCTATGGCTAATGAAG+TGG - chr3_4:76016128-76016147 Msa0522700:five_prime_UTR 50.0%
GTGAGAGGAGTGAGAGTGAC+TGG + chr3_4:76012957-76012976 None:intergenic 55.0%
! AGCGTGATTGAAGCACCCAC+TGG - chr3_4:76014277-76014296 Msa0522700:intron 55.0%
GGGTCCGTTACTATGCCTCA+TGG - chr3_4:76014966-76014985 Msa0522700:intron 55.0%
! GAGGCATTTCATGTCCCAGC+TGG - chr3_4:76015339-76015358 Msa0522700:intron 55.0%
GGTCGTAAGGGAAGTCTCCA+AGG - chr3_4:76015950-76015969 Msa0522700:five_prime_UTR 55.0%
! CATTAGCCATAGGGTGGTGG+CGG + chr3_4:76016125-76016144 None:intergenic 55.0%
GCCAATCCGCCACCACCCTA+TGG - chr3_4:76016116-76016135 Msa0522700:five_prime_UTR 65.0%
!! GCCATAGGGTGGTGGCGGAT+TGG + chr3_4:76016120-76016139 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 76012628 76016230 76012628 ID=Msa0522700;Name=Msa0522700
chr3_4 mRNA 76012628 76016230 76012628 ID=Msa0522700-mRNA-1;Parent=Msa0522700;Name=Msa0522700-mRNA-1;_AED=0.11;_eAED=0.11;_QI=523|1|1|1|1|1|6|509|230
chr3_4 exon 76012628 76013208 76012628 ID=Msa0522700-mRNA-1:exon:21898;Parent=Msa0522700-mRNA-1
chr3_4 exon 76013498 76013569 76013498 ID=Msa0522700-mRNA-1:exon:21897;Parent=Msa0522700-mRNA-1
chr3_4 exon 76013685 76013747 76013685 ID=Msa0522700-mRNA-1:exon:21896;Parent=Msa0522700-mRNA-1
chr3_4 exon 76013863 76014002 76013863 ID=Msa0522700-mRNA-1:exon:21895;Parent=Msa0522700-mRNA-1
chr3_4 exon 76014500 76014626 76014500 ID=Msa0522700-mRNA-1:exon:21894;Parent=Msa0522700-mRNA-1
chr3_4 exon 76015489 76016230 76015489 ID=Msa0522700-mRNA-1:exon:21893;Parent=Msa0522700-mRNA-1
chr3_4 five_prime_UTR 76015708 76016230 76015708 ID=Msa0522700-mRNA-1:five_prime_utr;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76015489 76015707 76015489 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76014500 76014626 76014500 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76013863 76014002 76013863 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76013685 76013747 76013685 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76013498 76013569 76013498 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 CDS 76013137 76013208 76013137 ID=Msa0522700-mRNA-1:cds;Parent=Msa0522700-mRNA-1
chr3_4 three_prime_UTR 76012628 76013136 76012628 ID=Msa0522700-mRNA-1:three_prime_utr;Parent=Msa0522700-mRNA-1
Gene Sequence

>Msa0522700

ATGATTGAAGCAATGGAAAGTTCAGTTAACGGTGGTGGTGGTTTCGCGACTCATTTGCAAAGCTTTGGTGGTGGTGGTGATAGTAGTGAAGAGGAACTGTCTGTGTTACCTCGTCATACTAAGGTTGTTGTTACTGGAAATAACCGTACAAAATCTGTTCTTGTTGGTCTTCAAGGCGTTGTTAAGAAAGCTGTTGGATTAGGTGGTTGGCATTGGCTGGTTCTTACTAATGGAATTGAAGTGAAGCTACAAAGGAATGCTCTTAGCGTGATTGAAGCACCCACTGGCAATGAAGAAGATGATGACCTTGAATTTGATAACGTGCAATGGAATGGATCAGATATGGCATCAGACGATACACACAAGTCTCACAAATCGAGGCACAGAATGCATAGATCATTGGGATCATGTCACAAGACCATGAGTAGGTCCTTCTCTGGTGATTCACAGTCTAAGGGGTCCGTTACTATGCCTCATGGGTCCATGAAGGTTGACTTGAGCAAGCTTGAAATGGCTGCATTGTGGAGATATTGGAGACATTTTAATCTTGTGGATGTCGTCCCAAACCCATCAAAGGAGCAGCTAGTTGATGTTGTTCAGAGGCATTTCATGTCCCAGCTGCAAATGGACGAGTTGCAGGTCATTAAGGGATTTGTCAATGCTGCAAAGAGGGTCAAGACTGTGTGCAAATGA
Protein sequence

>Msa0522700

MIEAMESSVNGGGGFATHLQSFGGGGDSSEEELSVLPRHTKVVVTGNNRTKSVLVGLQGVVKKAVGLGGWHWLVLTNGIEVKLQRNALSVIEAPTGNEEDDDLEFDNVQWNGSDMASDDTHKSHKSRHRMHRSLGSCHKTMSRSFSGDSQSKGSVTMPHGSMKVDLSKLEMAALWRYWRHFNLVDVVPNPSKEQLVDVVQRHFMSQLQMDELQVIKGFVNAAKRVKTVCK*