AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0346740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0346740 MtrunA17_Chr5g0397391 96.894 161 5 0 1 161 127 287 3.09e-112 320
Msa0346740 MtrunA17_Chr8g0386961 93.125 160 11 0 1 160 127 286 6.70e-108 309
Msa0346740 MtrunA17_Chr5g0436701 85.625 160 23 0 1 160 130 289 9.09e-99 286
Msa0346740 MtrunA17_Chr3g0113211 85.093 161 24 0 1 161 130 290 1.47e-96 280
Msa0346740 MtrunA17_Chr4g0006001 85.000 160 23 1 1 160 131 289 2.48e-96 280
Msa0346740 MtrunA17_Chr7g0264111 74.843 159 39 1 1 159 120 277 4.85e-84 248
Msa0346740 MtrunA17_Chr2g0326621 70.440 159 46 1 1 159 122 279 2.31e-80 239
Msa0346740 MtrunA17_Chr1g0199731 71.698 159 44 1 1 159 120 277 5.78e-80 238
Msa0346740 MtrunA17_Chr4g0029071 70.440 159 46 1 1 159 120 277 2.44e-77 231
Msa0346740 MtrunA17_Chr3g0145671 69.811 159 47 1 1 159 112 269 2.47e-76 229
Msa0346740 MtrunA17_Chr6g0478191 42.331 163 80 6 1 161 98 248 5.78e-22 88.6
Msa0346740 MtrunA17_Chr1g0188771 37.255 153 83 4 1 152 98 238 4.21e-20 84.0
Msa0346740 MtrunA17_Chr2g0331261 33.553 152 88 4 1 151 97 236 9.55e-17 74.7
Msa0346740 MtrunA17_Chr1g0212671 35.714 154 85 5 1 152 98 239 2.25e-16 73.9
Msa0346740 MtrunA17_Chr4g0030861 33.974 156 78 6 1 149 98 235 6.55e-16 72.4
Msa0346740 MtrunA17_Chr8g0340161 34.228 149 85 4 1 148 96 232 7.49e-16 72.4
Msa0346740 MtrunA17_Chr5g0399341 35.119 168 85 7 1 161 96 246 2.13e-15 70.9
Msa0346740 MtrunA17_Chr1g0146161 31.410 156 82 5 1 149 92 229 1.67e-14 68.6
Msa0346740 MtrunA17_Chr1g0146171 32.692 156 80 5 1 149 92 229 2.14e-14 68.2
Msa0346740 MtrunA17_Chr7g0264961 32.692 156 83 6 1 151 98 236 2.08e-13 65.9
Msa0346740 MtrunA17_Chr4g0038611 28.659 164 101 6 1 153 60 218 1.23e-12 63.9
Msa0346740 MtrunA17_Chr7g0222791 32.468 154 90 5 1 152 98 239 1.54e-12 63.2
Msa0346740 MtrunA17_Chr4g0038591 26.946 167 100 7 1 153 123 281 2.97e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0346740 AT1G01620.2 87.578 161 19 1 1 161 55 214 3.49e-100 287
Msa0346740 AT1G01620.1 87.578 161 19 1 1 161 127 286 1.44e-99 288
Msa0346740 AT4G23400.1 88.125 160 18 1 1 160 128 286 1.56e-98 285
Msa0346740 AT4G00430.1 86.335 161 21 1 1 161 128 287 5.99e-98 284
Msa0346740 AT3G61430.2 84.472 161 24 1 1 161 127 286 3.64e-97 282
Msa0346740 AT3G61430.1 84.472 161 24 1 1 161 127 286 3.64e-97 282
Msa0346740 AT2G45960.1 84.472 161 24 1 1 161 127 286 4.17e-96 279
Msa0346740 AT2G37170.2 74.026 154 39 1 1 154 161 313 1.05e-80 242
Msa0346740 AT2G37170.1 74.026 154 39 1 1 154 118 270 1.59e-80 240
Msa0346740 AT5G60660.1 72.956 159 42 1 1 159 120 277 1.78e-80 240
Msa0346740 AT3G53420.1 74.026 154 39 1 1 154 120 272 9.00e-80 238
Msa0346740 AT3G53420.2 74.026 154 39 1 1 154 120 272 9.00e-80 238
Msa0346740 AT2G39010.1 70.440 159 46 1 1 159 119 276 4.71e-79 236
Msa0346740 AT2G37180.1 72.727 154 41 1 1 154 118 270 8.36e-79 235
Msa0346740 AT3G54820.1 71.698 159 44 1 1 159 119 276 3.23e-78 234
Msa0346740 AT2G16850.1 71.069 159 45 1 1 159 111 268 2.16e-77 231
Msa0346740 AT4G35100.1 70.440 159 46 1 1 159 113 270 1.77e-76 229
Msa0346740 AT4G35100.2 70.440 159 46 1 1 159 113 270 1.77e-76 229
Msa0346740 AT2G45960.2 83.846 130 20 1 1 130 127 255 1.22e-74 224
Msa0346740 AT2G45960.3 83.846 130 20 1 1 130 127 255 1.04e-73 223
Msa0346740 AT4G00430.2 78.409 88 18 1 1 88 128 214 4.67e-41 137
Msa0346740 AT2G39010.2 64.286 84 29 1 1 84 119 201 6.34e-31 111
Msa0346740 AT1G17810.1 39.216 153 80 4 1 152 106 246 2.14e-21 87.8
Msa0346740 AT1G73190.1 40.909 154 77 5 5 156 110 251 1.10e-20 85.9
Msa0346740 AT5G47450.1 37.584 149 80 5 1 148 96 232 1.36e-17 77.4
Msa0346740 AT4G01470.1 36.538 156 78 5 1 151 98 237 3.37e-17 76.3
Msa0346740 AT4G17340.1 37.255 153 75 6 1 148 96 232 1.16e-16 74.7
Msa0346740 AT3G16240.1 33.557 149 86 4 1 148 96 232 1.69e-14 68.9
Msa0346740 AT2G36830.1 33.548 155 82 6 1 150 98 236 2.47e-14 68.6
Msa0346740 AT3G26520.1 33.333 156 83 5 1 151 99 238 1.02e-13 67.0
Msa0346740 AT2G25810.1 29.936 157 97 4 1 156 92 236 1.66e-13 66.2
Msa0346740 AT3G47440.1 31.373 153 91 4 1 152 100 239 3.74e-13 65.5

Find 39 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTACAATTCCCATTAC+TGG 0.294571 3_1:-7390016 Msa0346740:CDS
TTCTGGGTTGGACCTTTCAT+TGG 0.308099 3_1:-7389813 Msa0346740:CDS
GATCCGAGCAATTCCTTTCA+AGG 0.393867 3_1:-7389757 Msa0346740:CDS
GCTCGTTATGAGTTGTATAA+AGG 0.401946 3_1:-7390374 Msa0346740:CDS
ACAATTCCCATTACTGGAAC+TGG 0.407279 3_1:-7390010 Msa0346740:CDS
GAATTGCTCGGATCACTATC+TGG 0.408932 3_1:+7389766 None:intergenic
ATGGTCTCTGTTGTATATTA+TGG 0.412666 3_1:+7389958 None:intergenic
AGAGTCTCTTGCATTTCTCT+TGG 0.418783 3_1:+7390247 None:intergenic
TGCAAGAGACTCTCACGTTC+CGG 0.436085 3_1:-7390237 Msa0346740:intron
TACACCAAGGGTGATGGTCT+TGG 0.441240 3_1:-7390326 Msa0346740:CDS
CTTGGAGCTATCTGTGGTGC+TGG 0.453990 3_1:-7390422 Msa0346740:CDS
AATTACTCCCTTGCCTTGAA+AGG 0.455988 3_1:+7389744 None:intergenic
TTAATGCCAGTTCCAGTAAT+GGG 0.469401 3_1:+7390004 None:intergenic
TACATAATAATGCAGTGTCT+TGG 0.479630 3_1:-7390440 Msa0346740:CDS
GGTCTTGGAGCTGAGATTGT+TGG 0.502760 3_1:-7390311 Msa0346740:CDS
ATTAACCCAGCTAGGACTCT+TGG 0.510874 3_1:-7389986 Msa0346740:CDS
TGCATTTCTCTTGGCATCAG+TGG 0.515764 3_1:+7390256 None:intergenic
TATACAACAGAGACCATGCT+TGG 0.521813 3_1:-7389952 Msa0346740:CDS
AGCTCCAAGACCATCACCCT+TGG 0.522666 3_1:+7390322 None:intergenic
AGCAATTCCTTTCAAGGCAA+GGG 0.527223 3_1:-7389751 Msa0346740:CDS
CCATCACCCTTGGTGTAACC+AGG 0.535441 3_1:+7390332 None:intergenic
GCAGCACCAAGAGTCCTAGC+TGG 0.536664 3_1:+7389980 None:intergenic
TTGCCTTGAAAGGAATTGCT+CGG 0.539077 3_1:+7389754 None:intergenic
GCAAGTGCAGCTCCAATGAA+AGG 0.549715 3_1:+7389801 None:intergenic
GGAGCAAATGTTGTGACTCC+TGG 0.558145 3_1:-7390350 Msa0346740:CDS
GTTAATGCCAGTTCCAGTAA+TGG 0.566105 3_1:+7390003 None:intergenic
CCTGGTTACACCAAGGGTGA+TGG 0.585245 3_1:-7390332 Msa0346740:CDS
GAGCAATTCCTTTCAAGGCA+AGG 0.588614 3_1:-7389752 Msa0346740:CDS
CGTTATGAGTTGTATAAAGG+TGG 0.591045 3_1:-7390371 Msa0346740:CDS
CAGTGTCTTGGAGCTATCTG+TGG 0.593126 3_1:-7390428 Msa0346740:CDS
ACAGCAAACCCAATTGGAAG+AGG 0.593192 3_1:+7390052 None:intergenic
GTAGAATACTGCTCTTGTCA+AGG 0.594791 3_1:+7390460 None:intergenic
ATACAACAGAGACCATGCTT+GGG 0.595986 3_1:-7389951 Msa0346740:CDS
CAGCACCAAGAGTCCTAGCT+GGG 0.602891 3_1:+7389981 None:intergenic
TGTGGTGCTGGTGTAGTGAA+AGG 0.611215 3_1:-7390410 Msa0346740:CDS
ATACATGGTCATCCCAAGCA+TGG 0.639779 3_1:+7389939 None:intergenic
GAACTGGCATTAACCCAGCT+AGG 0.640680 3_1:-7389994 Msa0346740:CDS
GTGACTCCTGGTTACACCAA+GGG 0.652855 3_1:-7390338 Msa0346740:CDS
TGTGACTCCTGGTTACACCA+AGG 0.762315 3_1:-7390339 Msa0346740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTAATGTTTGAATTTTG+TGG - chr3_1:7390356-7390375 Msa0346740:CDS 15.0%
!!! ATGTTTGTAATTGTAGATTT+TGG - chr3_1:7390133-7390152 Msa0346740:intron 20.0%
!! CAGAACAAAAAATAAATTGA+AGG + chr3_1:7390325-7390344 None:intergenic 20.0%
!!! TGATATATGCTTCATTTTTG+TGG - chr3_1:7390094-7390113 Msa0346740:intron 25.0%
!!! AAATTAAACAGTGGATTTTC+TGG - chr3_1:7390380-7390399 Msa0346740:CDS 25.0%
!!! AATTAAACAGTGGATTTTCT+GGG - chr3_1:7390381-7390400 Msa0346740:CDS 25.0%
TACATAATAATGCAGTGTCT+TGG - chr3_1:7389770-7389789 Msa0346740:CDS 30.0%
TGAAATTCATTTACACGTAC+CGG + chr3_1:7389995-7390014 None:intergenic 30.0%
ATGGTCTCTGTTGTATATTA+TGG + chr3_1:7390255-7390274 None:intergenic 30.0%
! TTTTGTGGCAAATTAAACAG+TGG - chr3_1:7390371-7390390 Msa0346740:CDS 30.0%
GCTCGTTATGAGTTGTATAA+AGG - chr3_1:7389836-7389855 Msa0346740:intron 35.0%
CGTTATGAGTTGTATAAAGG+TGG - chr3_1:7389839-7389858 Msa0346740:intron 35.0%
AAAAACACAGCAAACCCAAT+TGG + chr3_1:7390167-7390186 None:intergenic 35.0%
!!! AATTGGGTTTGCTGTGTTTT+TGG - chr3_1:7390166-7390185 Msa0346740:intron 35.0%
TTAATGCCAGTTCCAGTAAT+GGG + chr3_1:7390209-7390228 None:intergenic 35.0%
ACAAAGTGAAAGGACATACA+TGG + chr3_1:7390289-7390308 None:intergenic 35.0%
CAACAGCATAACAAAGTGAA+AGG + chr3_1:7390299-7390318 None:intergenic 35.0%
! TTCACTTTGTTATGCTGTTG+TGG - chr3_1:7390299-7390318 Msa0346740:CDS 35.0%
GTAGAATACTGCTCTTGTCA+AGG + chr3_1:7389753-7389772 None:intergenic 40.0%
! TGGTGTAGTGAAAGGTTTTG+AGG - chr3_1:7389808-7389827 Msa0346740:CDS 40.0%
AGAGTCTCTTGCATTTCTCT+TGG + chr3_1:7389966-7389985 None:intergenic 40.0%
!! ATTTTGGCACCTCTTCCAAT+TGG - chr3_1:7390149-7390168 Msa0346740:intron 40.0%
! TTTTGGCACCTCTTCCAATT+GGG - chr3_1:7390150-7390169 Msa0346740:intron 40.0%
!!! TGCTGTGTTTTTGGTTCACT+TGG - chr3_1:7390175-7390194 Msa0346740:intron 40.0%
TTGGCTACAATTCCCATTAC+TGG - chr3_1:7390194-7390213 Msa0346740:intron 40.0%
ACAATTCCCATTACTGGAAC+TGG - chr3_1:7390200-7390219 Msa0346740:intron 40.0%
GTTAATGCCAGTTCCAGTAA+TGG + chr3_1:7390210-7390229 None:intergenic 40.0%
TATACAACAGAGACCATGCT+TGG - chr3_1:7390258-7390277 Msa0346740:CDS 40.0%
ATACAACAGAGACCATGCTT+GGG - chr3_1:7390259-7390278 Msa0346740:CDS 40.0%
!! AAACAGTGGATTTTCTGGGT+TGG - chr3_1:7390385-7390404 Msa0346740:CDS 40.0%
TTGCCTTGAAAGGAATTGCT+CGG + chr3_1:7390459-7390478 None:intergenic 40.0%
AGCAATTCCTTTCAAGGCAA+GGG - chr3_1:7390459-7390478 Msa0346740:CDS 40.0%
TGCATTTCTCTTGGCATCAG+TGG + chr3_1:7389957-7389976 None:intergenic 45.0%
ACAGCAAACCCAATTGGAAG+AGG + chr3_1:7390161-7390180 None:intergenic 45.0%
ATTAACCCAGCTAGGACTCT+TGG - chr3_1:7390224-7390243 Msa0346740:intron 45.0%
ATACATGGTCATCCCAAGCA+TGG + chr3_1:7390274-7390293 None:intergenic 45.0%
! TTCTGGGTTGGACCTTTCAT+TGG - chr3_1:7390397-7390416 Msa0346740:CDS 45.0%
GAATTGCTCGGATCACTATC+TGG + chr3_1:7390447-7390466 None:intergenic 45.0%
GATCCGAGCAATTCCTTTCA+AGG - chr3_1:7390453-7390472 Msa0346740:CDS 45.0%
GAGCAATTCCTTTCAAGGCA+AGG - chr3_1:7390458-7390477 Msa0346740:CDS 45.0%
CAGTGTCTTGGAGCTATCTG+TGG - chr3_1:7389782-7389801 Msa0346740:CDS 50.0%
!! TGTGGTGCTGGTGTAGTGAA+AGG - chr3_1:7389800-7389819 Msa0346740:CDS 50.0%
GGAGCAAATGTTGTGACTCC+TGG - chr3_1:7389860-7389879 Msa0346740:intron 50.0%
TGTGACTCCTGGTTACACCA+AGG - chr3_1:7389871-7389890 Msa0346740:intron 50.0%
GTGACTCCTGGTTACACCAA+GGG - chr3_1:7389872-7389891 Msa0346740:intron 50.0%
! TACACCAAGGGTGATGGTCT+TGG - chr3_1:7389884-7389903 Msa0346740:intron 50.0%
GGTCTTGGAGCTGAGATTGT+TGG - chr3_1:7389899-7389918 Msa0346740:intron 50.0%
TGCAAGAGACTCTCACGTTC+CGG - chr3_1:7389973-7389992 Msa0346740:CDS 50.0%
GAACTGGCATTAACCCAGCT+AGG - chr3_1:7390216-7390235 Msa0346740:intron 50.0%
GCAAGTGCAGCTCCAATGAA+AGG + chr3_1:7390412-7390431 None:intergenic 50.0%
CTTGGAGCTATCTGTGGTGC+TGG - chr3_1:7389788-7389807 Msa0346740:CDS 55.0%
! CCATCACCCTTGGTGTAACC+AGG + chr3_1:7389881-7389900 None:intergenic 55.0%
CCTGGTTACACCAAGGGTGA+TGG - chr3_1:7389878-7389897 Msa0346740:intron 55.0%
AGCTCCAAGACCATCACCCT+TGG + chr3_1:7389891-7389910 None:intergenic 55.0%
CAGCACCAAGAGTCCTAGCT+GGG + chr3_1:7390232-7390251 None:intergenic 55.0%
GCAGCACCAAGAGTCCTAGC+TGG + chr3_1:7390233-7390252 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 7389746 7390486 7389746 ID=Msa0346740;Name=Msa0346740
chr3_1 mRNA 7389746 7390486 7389746 ID=Msa0346740-mRNA-1;Parent=Msa0346740;Name=Msa0346740-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|3|0|161
chr3_1 exon 7390238 7390486 7390238 ID=Msa0346740-mRNA-1:exon:2095;Parent=Msa0346740-mRNA-1
chr3_1 exon 7389943 7390083 7389943 ID=Msa0346740-mRNA-1:exon:2094;Parent=Msa0346740-mRNA-1
chr3_1 exon 7389746 7389841 7389746 ID=Msa0346740-mRNA-1:exon:2093;Parent=Msa0346740-mRNA-1
chr3_1 CDS 7390238 7390486 7390238 ID=Msa0346740-mRNA-1:cds;Parent=Msa0346740-mRNA-1
chr3_1 CDS 7389943 7390083 7389943 ID=Msa0346740-mRNA-1:cds;Parent=Msa0346740-mRNA-1
chr3_1 CDS 7389746 7389841 7389746 ID=Msa0346740-mRNA-1:cds;Parent=Msa0346740-mRNA-1
Gene Sequence

>Msa0346740

CTGTCCTTGACAAGAGCAGTATTCTACATAATAATGCAGTGTCTTGGAGCTATCTGTGGTGCTGGTGTAGTGAAAGGTTTTGAGGCTAATGCTCGTTATGAGTTGTATAAAGGTGGAGCAAATGTTGTGACTCCTGGTTACACCAAGGGTGATGGTCTTGGAGCTGAGATTGTTGGTACTTTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGATGCCAAGAGAAATGCAAGAGACTCTCACGTTCCGATTTTGGCACCTCTTCCAATTGGGTTTGCTGTGTTTTTGGTTCACTTGGCTACAATTCCCATTACTGGAACTGGCATTAACCCAGCTAGGACTCTTGGTGCTGCCATAATATACAACAGAGACCATGCTTGGGATGACCATTGGATTTTCTGGGTTGGACCTTTCATTGGAGCTGCACTTGCTGCATTATATCACCAGATAGTGATCCGAGCAATTCCTTTCAAGGCAAGGGAGTAA

Protein sequence

>Msa0346740

LSLTRAVFYIIMQCLGAICGAGVVKGFEANARYELYKGGANVVTPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARTLGAAIIYNRDHAWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKARE*