AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0479010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0479010 MtrunA17_Chr8g0380721 93.590 78 5 0 1 78 179 256 5.39e-44 147
Msa0479010 MtrunA17_Chr8g0380701 78.205 78 17 0 1 78 152 229 1.61e-36 124
Msa0479010 MtrunA17_Chr4g0025011 78.667 75 16 0 1 75 77 151 9.00e-36 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0479010 AT1G61000.1 51.282 78 38 0 1 78 174 251 1.25e-21 87.4

Find 72 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTCTTAATCAGCCATTT+TGG 0.249434 3_3:-83826172 None:intergenic
ATGACATCTGTTTATTGTTC+AGG 0.250147 3_3:-83826879 None:intergenic
TGCATTTGCTCAATTGCTTT+GGG 0.264099 3_3:+83825641 Msa0479010:five_prime_UTR
GGCAAATTGTGCAGATAAAA+TGG 0.274667 3_3:+83826679 Msa0479010:five_prime_UTR
CTGCATTTGCTCAATTGCTT+TGG 0.275397 3_3:+83825640 Msa0479010:five_prime_UTR
GTGCTGGAAAATTCTTCTTT+TGG 0.284743 3_3:-83825962 None:intergenic
AATGATACCTGTACTTTATC+AGG 0.298393 3_3:-83827335 None:intergenic
CTGTAGATGATTTAAATGTT+TGG 0.301649 3_3:+83826080 Msa0479010:five_prime_UTR
GTCTGAATTGTGATAGTATT+CGG 0.311465 3_3:+83826020 Msa0479010:five_prime_UTR
ACACCAATACGCATAGTTAA+AGG 0.322478 3_3:-83829656 None:intergenic
AAGGGAAATGCCTTCTGTTC+AGG 0.323153 3_3:+83826813 Msa0479010:exon
AATGACTATCATTCTCTTTG+TGG 0.360858 3_3:-83825831 None:intergenic
CTATACTTGCGGCGCTGAAT+AGG 0.365832 3_3:-83825692 None:intergenic
AACCTTAGTATAACTGTTGT+TGG 0.383313 3_3:+83825922 Msa0479010:five_prime_UTR
AATGATAGTCATTGAGGAAA+TGG 0.392182 3_3:+83825841 Msa0479010:five_prime_UTR
AATCTATTGGTGCTTCTCAT+TGG 0.394864 3_3:+83825728 Msa0479010:five_prime_UTR
TGGATATGCTCGGACCAAAA+TGG 0.396223 3_3:+83826158 Msa0479010:five_prime_UTR
TGTTTGGAACAATATATCTA+AGG 0.397747 3_3:+83826096 Msa0479010:five_prime_UTR
AAAACTGTTGGCATTGCAAT+AGG 0.398141 3_3:-83825758 None:intergenic
GACTGTGGAAATGGATGATA+AGG 0.411995 3_3:+83826933 Msa0479010:CDS
TTCTGGTGCTGAATATAAAC+TGG 0.412690 3_3:+83827262 Msa0479010:CDS
CTATTCAGCGCCGCAAGTAT+AGG 0.427697 3_3:+83825693 Msa0479010:five_prime_UTR
TTGTCAGTTACGATGGCTAT+TGG 0.429901 3_3:+83825779 Msa0479010:five_prime_UTR
CTCCACAGAGCATTTGATAA+AGG 0.432915 3_3:-83829829 None:intergenic
GAAGGAGAGGACTGTGGAAA+TGG 0.440042 3_3:+83826924 Msa0479010:CDS
CGATTCTGCATGTTAAAGTC+TGG 0.442759 3_3:-83826209 None:intergenic
GATATTGTACAATTCTTGAA+AGG 0.450055 3_3:+83826794 Msa0479010:five_prime_UTR
GGCTGCAATGGTGGCTGCAA+TGG 0.454503 3_3:+83825862 Msa0479010:five_prime_UTR
AAGTACTATAAAGAAATTGA+AGG 0.472617 3_3:+83826906 Msa0479010:CDS
AAATGGCTGATTAAGAGTTA+AGG 0.474751 3_3:+83826175 Msa0479010:five_prime_UTR
CTATTGGTGCTTCTCATTGG+AGG 0.489832 3_3:+83825731 Msa0479010:five_prime_UTR
ATATTGTACAATTCTTGAAA+GGG 0.490218 3_3:+83826795 Msa0479010:five_prime_UTR
AACAACAGTTATACTAAGGT+TGG 0.501460 3_3:-83825920 None:intergenic
AGGCCTTTAACTATGCGTAT+TGG 0.506428 3_3:+83829653 Msa0479010:three_prime_UTR
TAGAAAAGAGCAAAGAGTGC+TGG 0.507946 3_3:-83825978 None:intergenic
CTCCAACAACAGTTATACTA+AGG 0.511684 3_3:-83825924 None:intergenic
TATTGGTGCTTCTCATTGGA+GGG 0.514746 3_3:+83825732 Msa0479010:five_prime_UTR
TTAACTATGCGTATTGGTGT+TGG 0.521341 3_3:+83829659 Msa0479010:three_prime_UTR
AAAGAGAATGATAGTCATTG+AGG 0.526397 3_3:+83825835 Msa0479010:five_prime_UTR
ATAGTAGTTATAAACGTGCT+AGG 0.527374 3_3:+83829633 Msa0479010:three_prime_UTR
GATCATGTGTTGACAGCCAT+CGG 0.527698 3_3:+83825611 Msa0479010:five_prime_UTR
AATGCCTTCTGTTCAGGAGG+TGG 0.532743 3_3:+83826819 Msa0479010:exon
GTACAAAATGTTCAAGAGCA+TGG 0.533679 3_3:+83827284 Msa0479010:CDS
GAGTGGAATTGACAATCTAT+TGG 0.533730 3_3:+83825715 Msa0479010:five_prime_UTR
ACTATCACAATTCAGACATC+GGG 0.534239 3_3:-83826014 None:intergenic
TTATTCCGTTTGGATATGCT+CGG 0.539101 3_3:+83826148 Msa0479010:five_prime_UTR
CAATTCCACTCCTATACTTG+CGG 0.549747 3_3:-83825703 None:intergenic
GCATCCACCTCCTGAACAGA+AGG 0.551705 3_3:-83826823 None:intergenic
AATTGTACAATATCAACTCC+AGG 0.560612 3_3:-83826785 None:intergenic
TTGGTCCGAGCATATCCAAA+CGG 0.569946 3_3:-83826153 None:intergenic
CGATGGCTATTGGTTCACAT+AGG 0.572428 3_3:+83825789 Msa0479010:five_prime_UTR
ATCGTAACTGACAAAACTGT+TGG 0.576198 3_3:-83825770 None:intergenic
TACTATCACAATTCAGACAT+CGG 0.579861 3_3:-83826015 None:intergenic
TTGTCACACTCTGAATCCGC+AGG 0.583371 3_3:+83825895 Msa0479010:five_prime_UTR
ACTATCAAATACTATTCCAT+CGG 0.594477 3_3:-83829615 None:intergenic
CTATAAAGAAATTGAAGGAG+AGG 0.594531 3_3:+83826911 Msa0479010:CDS
GAAATTGAAGGAGAGGACTG+TGG 0.597307 3_3:+83826918 Msa0479010:CDS
TATACTAAGGTTGGATCCTG+CGG 0.606504 3_3:-83825911 None:intergenic
TGAGGAAATGGCTGCAATGG+TGG 0.607777 3_3:+83825853 Msa0479010:five_prime_UTR
CATTGAGGAAATGGCTGCAA+TGG 0.609889 3_3:+83825850 Msa0479010:five_prime_UTR
TCAATCGCCTGATAAAGTAC+AGG 0.611191 3_3:+83827328 Msa0479010:CDS
TTCCTTTATCAAATGCTCTG+TGG 0.615221 3_3:+83829827 Msa0479010:three_prime_UTR
CAGAGTTGTACAGACCAGAA+TGG 0.623697 3_3:-83826727 None:intergenic
TATGCGTATTGGTGTTGGCA+AGG 0.624457 3_3:+83829664 Msa0479010:three_prime_UTR
TCAACACATGATCAAAGCCA+TGG 0.635234 3_3:-83825601 None:intergenic
CAGCGCCGCAAGTATAGGAG+TGG 0.639208 3_3:+83825698 Msa0479010:five_prime_UTR
GGAAATGCCTTCTGTTCAGG+AGG 0.641002 3_3:+83826816 Msa0479010:exon
TAACATGCAGAATCGACAAG+AGG 0.642308 3_3:+83826217 Msa0479010:five_prime_UTR
TCACATAGGTTGTCTCAGCA+AGG 0.643329 3_3:+83825803 Msa0479010:five_prime_UTR
GCAAATGCAGAAGTTACCGA+TGG 0.680742 3_3:-83825627 None:intergenic
TATACAGCTAGATTCGCCGA+TGG 0.689447 3_3:+83829599 Msa0479010:CDS
TTCTGGTCTGTACAACTCTG+AGG 0.704995 3_3:+83826730 Msa0479010:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATTATGTAATATGA+AGG - chr3_3:83827697-83827716 None:intergenic 10.0%
!! TTTATTGTATTAAAAAATTG+TGG + chr3_3:83827711-83827730 Msa0479010:intron 10.0%
!! ATGTTTATGTGAATAATTAA+TGG + chr3_3:83827064-83827083 Msa0479010:intron 15.0%
!! ATATATATTAATTCATGTGT+TGG + chr3_3:83827166-83827185 Msa0479010:intron 15.0%
!!! ATATTGTAAATGTGTTTTAT+TGG + chr3_3:83827464-83827483 Msa0479010:intron 15.0%
!!! GTATAATACAAAAATTTTAG+AGG + chr3_3:83828380-83828399 Msa0479010:intron 15.0%
!! ATAATTAAGATGAAACTAAT+TGG - chr3_3:83829066-83829085 None:intergenic 15.0%
!!! TCGTTTTCTTTTAATTATTT+AGG + chr3_3:83829356-83829375 Msa0479010:intron 15.0%
!!! CGATACTATTTATTTTTTTA+TGG + chr3_3:83829449-83829468 Msa0479010:intron 15.0%
!!! ATCCAACTTTTTGATATATT+AGG - chr3_3:83825489-83825508 None:intergenic 20.0%
!! GGTAAATGAATATTACAATT+TGG - chr3_3:83826390-83826409 None:intergenic 20.0%
!!! ATTAATTGCATATTTGAAGT+CGG + chr3_3:83826424-83826443 Msa0479010:intron 20.0%
!! ATCAAATTTCAACATCAATT+TGG - chr3_3:83826458-83826477 None:intergenic 20.0%
!! AAAAGAAATCATTAGTATAG+GGG - chr3_3:83826650-83826669 None:intergenic 20.0%
!! CAAAAGAAATCATTAGTATA+GGG - chr3_3:83826651-83826670 None:intergenic 20.0%
!! TCAAAAGAAATCATTAGTAT+AGG - chr3_3:83826652-83826671 None:intergenic 20.0%
!!! AATGATTTCTTTTGAATTGT+AGG + chr3_3:83826657-83826676 Msa0479010:intron 20.0%
!!! ATGATTTCTTTTGAATTGTA+GGG + chr3_3:83826658-83826677 Msa0479010:intron 20.0%
!! ATATTGTACAATTCTTGAAA+GGG + chr3_3:83826795-83826814 Msa0479010:five_prime_UTR 20.0%
!! AAGTACTATAAAGAAATTGA+AGG + chr3_3:83826906-83826925 Msa0479010:CDS 20.0%
!! TTTATGTGAATAATTAATGG+TGG + chr3_3:83827067-83827086 Msa0479010:intron 20.0%
!! TAGTTAAGTAGTTATTTAAG+AGG + chr3_3:83827853-83827872 Msa0479010:intron 20.0%
!!! TATTTAAGAGGTTAGTTAAT+TGG + chr3_3:83827865-83827884 Msa0479010:intron 20.0%
!!! TTAGTAGATTTTTCATGAAA+AGG + chr3_3:83827899-83827918 Msa0479010:intron 20.0%
!! TAAAAAGATTATCAACAGTT+AGG + chr3_3:83828346-83828365 Msa0479010:intron 20.0%
!!! CATTGAGTTCATTTTATTTA+TGG + chr3_3:83828586-83828605 Msa0479010:intron 20.0%
!! AATAAGCAGGATTATAATTT+GGG - chr3_3:83828632-83828651 None:intergenic 20.0%
!! AAATAAGCAGGATTATAATT+TGG - chr3_3:83828633-83828652 None:intergenic 20.0%
!!! TAATTATTATGATGTTTTCC+TGG + chr3_3:83829079-83829098 Msa0479010:intron 20.0%
!!! TTTTATTTCTTGCTACATTT+TGG + chr3_3:83829242-83829261 Msa0479010:intron 20.0%
! TGCCTAATATATCAAAAAGT+TGG + chr3_3:83825484-83825503 Msa0479010:five_prime_UTR 25.0%
! CTGTAGATGATTTAAATGTT+TGG + chr3_3:83826080-83826099 Msa0479010:five_prime_UTR 25.0%
!! TGTTTGGAACAATATATCTA+AGG + chr3_3:83826096-83826115 Msa0479010:five_prime_UTR 25.0%
! GTTATAGTAGTAATTCTCTT+TGG + chr3_3:83826245-83826264 Msa0479010:intron 25.0%
! TTGTACAAAAGTTGCTAAAA+AGG + chr3_3:83826279-83826298 Msa0479010:intron 25.0%
! CTAACTATTCATTATAGTAG+AGG + chr3_3:83826310-83826329 Msa0479010:intron 25.0%
! AATTTCAACATCAATTTGGA+TGG - chr3_3:83826454-83826473 None:intergenic 25.0%
! TCATTTCACGAAAAATCAAT+AGG + chr3_3:83826500-83826519 Msa0479010:intron 25.0%
! TCTTACTAACGACTTAAAAA+AGG - chr3_3:83826557-83826576 None:intergenic 25.0%
! GATATTGTACAATTCTTGAA+AGG + chr3_3:83826794-83826813 Msa0479010:five_prime_UTR 25.0%
! TAATCTTTACTTCTTGATGT+TGG + chr3_3:83826998-83827017 Msa0479010:intron 25.0%
! AATCTTTACTTCTTGATGTT+GGG + chr3_3:83826999-83827018 Msa0479010:intron 25.0%
!!! TGAATAATTAATGGTGGATT+AGG + chr3_3:83827073-83827092 Msa0479010:intron 25.0%
! ATGAAGGACATAAATTATGT+TGG - chr3_3:83827681-83827700 None:intergenic 25.0%
!! ATGAGCTATTCTTTTATGTT+GGG - chr3_3:83827778-83827797 None:intergenic 25.0%
!! TATGAGCTATTCTTTTATGT+TGG - chr3_3:83827779-83827798 None:intergenic 25.0%
!! AGGTTAGTTAATTGGTTAAT+GGG + chr3_3:83827873-83827892 Msa0479010:intron 25.0%
! ATTCAAACATGCGTTAAAAT+TGG + chr3_3:83828172-83828191 Msa0479010:intron 25.0%
! TTACTCTATGAATAAGTTTC+CGG - chr3_3:83828310-83828329 None:intergenic 25.0%
!!! ATAGTTCTAAATTGCGATTA+CGG - chr3_3:83828488-83828507 None:intergenic 25.0%
! ATCAAAATGAGAAAATAAGC+AGG - chr3_3:83828645-83828664 None:intergenic 25.0%
!!! CATTTTGATACTTGATATCA+TGG + chr3_3:83828656-83828675 Msa0479010:intron 25.0%
!! AAGTTAAAATGTCATTTGAG+TGG + chr3_3:83828832-83828851 Msa0479010:intron 25.0%
!! AGTTAAAATGTCATTTGAGT+GGG + chr3_3:83828833-83828852 Msa0479010:intron 25.0%
! ATGAGAAAAAGGAATTTGAA+TGG - chr3_3:83828896-83828915 None:intergenic 25.0%
! GACAACAACGAAATTATATT+TGG - chr3_3:83829430-83829449 None:intergenic 25.0%
!!! TTTTTTATGGTGTTTCCTTT+AGG + chr3_3:83829462-83829481 Msa0479010:intron 25.0%
! ACTATCAAATACTATTCCAT+CGG - chr3_3:83829618-83829637 None:intergenic 25.0%
! AATTCAAACTCCAAATTAAC+TGG - chr3_3:83829787-83829806 None:intergenic 25.0%
AATGACTATCATTCTCTTTG+TGG - chr3_3:83825834-83825853 None:intergenic 30.0%
AAAGAGAATGATAGTCATTG+AGG + chr3_3:83825835-83825854 Msa0479010:five_prime_UTR 30.0%
AATGATAGTCATTGAGGAAA+TGG + chr3_3:83825841-83825860 Msa0479010:five_prime_UTR 30.0%
AACAACAGTTATACTAAGGT+TGG - chr3_3:83825923-83825942 None:intergenic 30.0%
AACCTTAGTATAACTGTTGT+TGG + chr3_3:83825922-83825941 Msa0479010:five_prime_UTR 30.0%
! TTGCTCTTTTCTATGAAGAT+TGG + chr3_3:83825988-83826007 Msa0479010:five_prime_UTR 30.0%
TACTATCACAATTCAGACAT+CGG - chr3_3:83826018-83826037 None:intergenic 30.0%
GTCTGAATTGTGATAGTATT+CGG + chr3_3:83826020-83826039 Msa0479010:five_prime_UTR 30.0%
! ATGCAGAATTTTATTCCGTT+TGG + chr3_3:83826138-83826157 Msa0479010:five_prime_UTR 30.0%
! TAACTCTTAATCAGCCATTT+TGG - chr3_3:83826175-83826194 None:intergenic 30.0%
AAATGGCTGATTAAGAGTTA+AGG + chr3_3:83826175-83826194 Msa0479010:five_prime_UTR 30.0%
GTAGAGGTTAAAAAGATTTC+AGG + chr3_3:83826326-83826345 Msa0479010:intron 30.0%
AATTAATCATGTTCAGTGGA+TGG - chr3_3:83826411-83826430 None:intergenic 30.0%
ATGCAATTAATCATGTTCAG+TGG - chr3_3:83826415-83826434 None:intergenic 30.0%
TGATTGATCAACAAAAAACC+TGG + chr3_3:83826767-83826786 Msa0479010:five_prime_UTR 30.0%
AATTGTACAATATCAACTCC+AGG - chr3_3:83826788-83826807 None:intergenic 30.0%
ATGACATCTGTTTATTGTTC+AGG - chr3_3:83826882-83826901 None:intergenic 30.0%
CTATAAAGAAATTGAAGGAG+AGG + chr3_3:83826911-83826930 Msa0479010:CDS 30.0%
ATCTTTACTTCTTGATGTTG+GGG + chr3_3:83827000-83827019 Msa0479010:intron 30.0%
! TTAATTCATGTGTTGGTACT+TGG + chr3_3:83827173-83827192 Msa0479010:intron 30.0%
AATGATACCTGTACTTTATC+AGG - chr3_3:83827338-83827357 None:intergenic 30.0%
! TACAGGTATCATTTTCATGT+TGG + chr3_3:83827345-83827364 Msa0479010:intron 30.0%
CACATTTACAATATTCACAC+AGG - chr3_3:83827458-83827477 None:intergenic 30.0%
! GAGGTTAGTTAATTGGTTAA+TGG + chr3_3:83827872-83827891 Msa0479010:intron 30.0%
AACAAATTCAGATGTGTCAA+AGG + chr3_3:83827926-83827945 Msa0479010:intron 30.0%
TCGAGTTTAGTAAAATCACT+GGG - chr3_3:83828147-83828166 None:intergenic 30.0%
ATCGAGTTTAGTAAAATCAC+TGG - chr3_3:83828148-83828167 None:intergenic 30.0%
!! AATTGTGATTTTAGCCGTAT+TGG + chr3_3:83828421-83828440 Msa0479010:intron 30.0%
! GTAATCGCAATTTAGAACTA+TGG + chr3_3:83828487-83828506 Msa0479010:intron 30.0%
AATATGCAGATTGATGTATG+AGG + chr3_3:83828528-83828547 Msa0479010:intron 30.0%
AATATTGTTAGACGAAGGTT+AGG - chr3_3:83828757-83828776 None:intergenic 30.0%
ATTAGAGGTTCATAATCATG+TGG + chr3_3:83828774-83828793 Msa0479010:intron 30.0%
! GTTAAAATGTCATTTGAGTG+GGG + chr3_3:83828834-83828853 Msa0479010:intron 30.0%
TAGATCACACTTTAATCTCT+CGG - chr3_3:83828870-83828889 None:intergenic 30.0%
!! TGTGTTATTTACACATGAGA+GGG - chr3_3:83828960-83828979 None:intergenic 30.0%
!! ATGTGTTATTTACACATGAG+AGG - chr3_3:83828961-83828980 None:intergenic 30.0%
AATCATAATCCAGACATCAT+TGG + chr3_3:83829026-83829045 Msa0479010:intron 30.0%
AAACTAATTGGAACCAATGA+AGG - chr3_3:83829054-83829073 None:intergenic 30.0%
AAGTCTTGATTCATGAAAAG+AGG - chr3_3:83829124-83829143 None:intergenic 30.0%
! TCTTTTCATGAATCAAGACT+TGG + chr3_3:83829123-83829142 Msa0479010:intron 30.0%
!!! TTTGGTTTTGTTGTAGTGTT+AGG + chr3_3:83829260-83829279 Msa0479010:intron 30.0%
CAACGAAATTATATTTGGAC+TGG - chr3_3:83829425-83829444 None:intergenic 30.0%
ATAGTAGTTATAAACGTGCT+AGG + chr3_3:83829633-83829652 Msa0479010:three_prime_UTR 30.0%
! GAACTGCAATTTTATGTCCA+TGG + chr3_3:83825584-83825603 Msa0479010:five_prime_UTR 35.0%
TGCATTTGCTCAATTGCTTT+GGG + chr3_3:83825641-83825660 Msa0479010:five_prime_UTR 35.0%
GAGTGGAATTGACAATCTAT+TGG + chr3_3:83825715-83825734 Msa0479010:five_prime_UTR 35.0%
! AATCTATTGGTGCTTCTCAT+TGG + chr3_3:83825728-83825747 Msa0479010:five_prime_UTR 35.0%
AAAACTGTTGGCATTGCAAT+AGG - chr3_3:83825761-83825780 None:intergenic 35.0%
ATCGTAACTGACAAAACTGT+TGG - chr3_3:83825773-83825792 None:intergenic 35.0%
!! AACAGTTTTGTCAGTTACGA+TGG + chr3_3:83825772-83825791 Msa0479010:five_prime_UTR 35.0%
CTCCAACAACAGTTATACTA+AGG - chr3_3:83825927-83825946 None:intergenic 35.0%
! GGAAAATTCTTCTTTTGGCA+AGG - chr3_3:83825960-83825979 None:intergenic 35.0%
! GTGCTGGAAAATTCTTCTTT+TGG - chr3_3:83825965-83825984 None:intergenic 35.0%
ACTATCACAATTCAGACATC+GGG - chr3_3:83826017-83826036 None:intergenic 35.0%
TTATTCCGTTTGGATATGCT+CGG + chr3_3:83826148-83826167 Msa0479010:five_prime_UTR 35.0%
AAGTCGTTAGTAAGACTCAT+CGG + chr3_3:83826562-83826581 Msa0479010:intron 35.0%
GCACATTTGTCATGTTATTG+TGG + chr3_3:83826592-83826611 Msa0479010:intron 35.0%
TAGGGGAAAAGTAAGAAAGA+AGG - chr3_3:83826633-83826652 None:intergenic 35.0%
GGCAAATTGTGCAGATAAAA+TGG + chr3_3:83826679-83826698 Msa0479010:five_prime_UTR 35.0%
! AAACGATGCTTTTACCATTC+TGG + chr3_3:83826713-83826732 Msa0479010:five_prime_UTR 35.0%
!!! TGGGGTTTTTGAGTAGTATA+TGG + chr3_3:83827018-83827037 Msa0479010:intron 35.0%
! CAGAAATCTGAGGTAACAAA+AGG - chr3_3:83827247-83827266 None:intergenic 35.0%
! CTTTTGTTACCTCAGATTTC+TGG + chr3_3:83827245-83827264 Msa0479010:intron 35.0%
! TTCTGGTGCTGAATATAAAC+TGG + chr3_3:83827262-83827281 Msa0479010:CDS 35.0%
GTACAAAATGTTCAAGAGCA+TGG + chr3_3:83827284-83827303 Msa0479010:CDS 35.0%
CAATATTCACACAGGAAGAT+GGG - chr3_3:83827450-83827469 None:intergenic 35.0%
ACAATATTCACACAGGAAGA+TGG - chr3_3:83827451-83827470 None:intergenic 35.0%
ACACCAAAGTCACAACATAA+TGG + chr3_3:83827807-83827826 Msa0479010:intron 35.0%
!! ACTCTGATTTTGGCACAATT+TGG + chr3_3:83828099-83828118 Msa0479010:intron 35.0%
! TAATTAGAGTACCTTTGTGC+AGG + chr3_3:83828210-83828229 Msa0479010:intron 35.0%
ACGGCAAAATACATCCAATA+CGG - chr3_3:83828438-83828457 None:intergenic 35.0%
CATTGCGGACAATTATATCA+CGG - chr3_3:83828457-83828476 None:intergenic 35.0%
!!! ATCATCCTGTTTCTGCATTT+TGG + chr3_3:83828681-83828700 Msa0479010:intron 35.0%
CCTCTAATATTGTTAGACGA+AGG - chr3_3:83828762-83828781 None:intergenic 35.0%
CCTTCGTCTAACAATATTAG+AGG + chr3_3:83828759-83828778 Msa0479010:intron 35.0%
CCAAAATGCACGACTAAATT+GGG - chr3_3:83828997-83829016 None:intergenic 35.0%
! CATATTTTGTTCATGAGACC+AGG - chr3_3:83829100-83829119 None:intergenic 35.0%
GTATAGTGTAACTTCTGCTT+TGG + chr3_3:83829211-83829230 Msa0479010:intron 35.0%
TTAGGAGTAGATCTCTAATG+TGG + chr3_3:83829290-83829309 Msa0479010:intron 35.0%
TTCCAGTCAAATCAATCGTT+GGG - chr3_3:83829332-83829351 None:intergenic 35.0%
ACATCAATAAGCTCTCCTAA+AGG - chr3_3:83829480-83829499 None:intergenic 35.0%
ACACCAATACGCATAGTTAA+AGG - chr3_3:83829659-83829678 None:intergenic 35.0%
! TTAACTATGCGTATTGGTGT+TGG + chr3_3:83829659-83829678 Msa0479010:three_prime_UTR 35.0%
! GCTGCTAATTTTTGTGAACT+TGG + chr3_3:83829761-83829780 Msa0479010:intron 35.0%
GATAAAGGAACATCTGCAAA+AGG - chr3_3:83829817-83829836 None:intergenic 35.0%
TTCCTTTATCAAATGCTCTG+TGG + chr3_3:83829827-83829846 Msa0479010:three_prime_UTR 35.0%
TCAACACATGATCAAAGCCA+TGG - chr3_3:83825604-83825623 None:intergenic 40.0%
CTGCATTTGCTCAATTGCTT+TGG + chr3_3:83825640-83825659 Msa0479010:five_prime_UTR 40.0%
CAATTCCACTCCTATACTTG+CGG - chr3_3:83825706-83825725 None:intergenic 40.0%
! TATTGGTGCTTCTCATTGGA+GGG + chr3_3:83825732-83825751 Msa0479010:five_prime_UTR 40.0%
TTGTCAGTTACGATGGCTAT+TGG + chr3_3:83825779-83825798 Msa0479010:five_prime_UTR 40.0%
TATACTAAGGTTGGATCCTG+CGG - chr3_3:83825914-83825933 None:intergenic 40.0%
! TAGAAAAGAGCAAAGAGTGC+TGG - chr3_3:83825981-83826000 None:intergenic 40.0%
CGATTCTGCATGTTAAAGTC+TGG - chr3_3:83826212-83826231 None:intergenic 40.0%
TAACATGCAGAATCGACAAG+AGG + chr3_3:83826217-83826236 Msa0479010:five_prime_UTR 40.0%
GACTGTGGAAATGGATGATA+AGG + chr3_3:83826933-83826952 Msa0479010:CDS 40.0%
TGCACGTTACATAACTGACA+GGG - chr3_3:83827107-83827126 None:intergenic 40.0%
TATTCAGCACCAGAAATCTG+AGG - chr3_3:83827257-83827276 None:intergenic 40.0%
TCAATCGCCTGATAAAGTAC+AGG + chr3_3:83827328-83827347 Msa0479010:CDS 40.0%
TGGTAACCACATAGAGGTAA+GGG - chr3_3:83827494-83827513 None:intergenic 40.0%
GTTAATGTTAGCATGTGCAC+CGG - chr3_3:83827561-83827580 None:intergenic 40.0%
! TATGTTACAGCAAGCTTGCT+AGG - chr3_3:83827599-83827618 None:intergenic 40.0%
CAGCCATTATGTTGTGACTT+TGG - chr3_3:83827813-83827832 None:intergenic 40.0%
CAACATAATGGCTGCTTGAT+AGG + chr3_3:83827819-83827838 Msa0479010:intron 40.0%
AGTACCTTTGTGCAGGATTA+TGG + chr3_3:83828217-83828236 Msa0479010:intron 40.0%
TCTTCCATAATCCTGCACAA+AGG - chr3_3:83828224-83828243 None:intergenic 40.0%
TTTGTGCAGGATTATGGAAG+AGG + chr3_3:83828223-83828242 Msa0479010:intron 40.0%
AGAGGAGAGCAACTCTAATT+TGG + chr3_3:83828241-83828260 Msa0479010:intron 40.0%
AGGACAAACTTTGCTCTTAG+GGG - chr3_3:83828282-83828301 None:intergenic 40.0%
CAGGACAAACTTTGCTCTTA+GGG - chr3_3:83828283-83828302 None:intergenic 40.0%
ACAGGACAAACTTTGCTCTT+AGG - chr3_3:83828284-83828303 None:intergenic 40.0%
TGAATAAGTTTCCGGCATAC+AGG - chr3_3:83828302-83828321 None:intergenic 40.0%
!! TAGAGGTCTCTAAAACTGCA+CGG + chr3_3:83828397-83828416 Msa0479010:intron 40.0%
TAATTGTCCGCAATGTCACT+AGG + chr3_3:83828462-83828481 Msa0479010:intron 40.0%
ATCACACTTTAATCTCTCGG+AGG - chr3_3:83828867-83828886 None:intergenic 40.0%
! GAGAGAAGCACATGAGAAAA+AGG - chr3_3:83828907-83828926 None:intergenic 40.0%
TGCTTCTCTCTCTTGATGAA+TGG + chr3_3:83828917-83828936 Msa0479010:intron 40.0%
! CCCAATTTAGTCGTGCATTT+TGG + chr3_3:83828994-83829013 Msa0479010:intron 40.0%
GCCAAAATGCACGACTAAAT+TGG - chr3_3:83828998-83829017 None:intergenic 40.0%
ATGAAGGAACCAATGATGTC+TGG - chr3_3:83829038-83829057 None:intergenic 40.0%
!! GACATCATTGGTTCCTTCAT+TGG + chr3_3:83829038-83829057 Msa0479010:intron 40.0%
GTAGTGTTAGGCTAACTCTT+AGG + chr3_3:83829272-83829291 Msa0479010:intron 40.0%
GATCTCTAATGTGGAGCAAT+AGG + chr3_3:83829299-83829318 Msa0479010:intron 40.0%
GTTCCAGTCAAATCAATCGT+TGG - chr3_3:83829333-83829352 None:intergenic 40.0%
AGGCCTTTAACTATGCGTAT+TGG + chr3_3:83829653-83829672 Msa0479010:three_prime_UTR 40.0%
GTGAACTTGGCCAGTTAATT+TGG + chr3_3:83829774-83829793 Msa0479010:intron 40.0%
! CTCCACAGAGCATTTGATAA+AGG - chr3_3:83829832-83829851 None:intergenic 40.0%
! GATCATGTGTTGACAGCCAT+CGG + chr3_3:83825611-83825630 Msa0479010:five_prime_UTR 45.0%
GCAAATGCAGAAGTTACCGA+TGG - chr3_3:83825630-83825649 None:intergenic 45.0%
! CTATTGGTGCTTCTCATTGG+AGG + chr3_3:83825731-83825750 Msa0479010:five_prime_UTR 45.0%
!! CGATGGCTATTGGTTCACAT+AGG + chr3_3:83825789-83825808 Msa0479010:five_prime_UTR 45.0%
TCACATAGGTTGTCTCAGCA+AGG + chr3_3:83825803-83825822 Msa0479010:five_prime_UTR 45.0%
CATTGAGGAAATGGCTGCAA+TGG + chr3_3:83825850-83825869 Msa0479010:five_prime_UTR 45.0%
TTGGTCCGAGCATATCCAAA+CGG - chr3_3:83826156-83826175 None:intergenic 45.0%
TGGATATGCTCGGACCAAAA+TGG + chr3_3:83826158-83826177 Msa0479010:five_prime_UTR 45.0%
CAGAGTTGTACAGACCAGAA+TGG - chr3_3:83826730-83826749 None:intergenic 45.0%
TTCTGGTCTGTACAACTCTG+AGG + chr3_3:83826730-83826749 Msa0479010:five_prime_UTR 45.0%
AAGGGAAATGCCTTCTGTTC+AGG + chr3_3:83826813-83826832 Msa0479010:exon 45.0%
GAAATTGAAGGAGAGGACTG+TGG + chr3_3:83826918-83826937 Msa0479010:CDS 45.0%
GTGCACGTTACATAACTGAC+AGG - chr3_3:83827108-83827127 None:intergenic 45.0%
ATTCACACAGGAAGATGGGT+TGG - chr3_3:83827446-83827465 None:intergenic 45.0%
GTGGTAACCACATAGAGGTA+AGG - chr3_3:83827495-83827514 None:intergenic 45.0%
!!! CTTCTTGTTTTGCTAAGCGC+AGG - chr3_3:83827625-83827644 None:intergenic 45.0%
TTGTGCACAGTCATAGCAAG+TGG - chr3_3:83828021-83828040 None:intergenic 45.0%
! CTTGCTATGACTGTGCACAA+AGG + chr3_3:83828021-83828040 Msa0479010:intron 45.0%
! CATACGCGAGACTCTGATTT+TGG + chr3_3:83828089-83828108 Msa0479010:intron 45.0%
ACAAACTTTGCTCTTAGGGG+TGG - chr3_3:83828279-83828298 None:intergenic 45.0%
GCAAAGTTTGTCCTGTATGC+CGG + chr3_3:83828288-83828307 Msa0479010:intron 45.0%
ATTACGGCCTAGTGACATTG+CGG - chr3_3:83828472-83828491 None:intergenic 45.0%
TGCAGCCAAAATGCAGAAAC+AGG - chr3_3:83828689-83828708 None:intergenic 45.0%
ACACATGAGAGGGTAAAGTG+TGG - chr3_3:83828950-83828969 None:intergenic 45.0%
!! GACCCAACGATTGATTTGAC+TGG + chr3_3:83829327-83829346 Msa0479010:intron 45.0%
TATACAGCTAGATTCGCCGA+TGG + chr3_3:83829599-83829618 Msa0479010:CDS 45.0%
!! TATGCGTATTGGTGTTGGCA+AGG + chr3_3:83829664-83829683 Msa0479010:three_prime_UTR 45.0%
! CTATACTTGCGGCGCTGAAT+AGG - chr3_3:83825695-83825714 None:intergenic 50.0%
CTATTCAGCGCCGCAAGTAT+AGG + chr3_3:83825693-83825712 Msa0479010:five_prime_UTR 50.0%
TGAGGAAATGGCTGCAATGG+TGG + chr3_3:83825853-83825872 Msa0479010:five_prime_UTR 50.0%
TTGTCACACTCTGAATCCGC+AGG + chr3_3:83825895-83825914 Msa0479010:five_prime_UTR 50.0%
GGAAATGCCTTCTGTTCAGG+AGG + chr3_3:83826816-83826835 Msa0479010:exon 50.0%
AATGCCTTCTGTTCAGGAGG+TGG + chr3_3:83826819-83826838 Msa0479010:exon 50.0%
GAAGGAGAGGACTGTGGAAA+TGG + chr3_3:83826924-83826943 Msa0479010:CDS 50.0%
CCACTGTGGTAACCACATAG+AGG - chr3_3:83827500-83827519 None:intergenic 50.0%
CCTCTATGTGGTTACCACAG+TGG + chr3_3:83827497-83827516 Msa0479010:intron 50.0%
GCGACTCACAACCTTCTCTT+GGG + chr3_3:83827529-83827548 Msa0479010:intron 50.0%
ACCTTCTCTTGGGTAGTGAC+CGG + chr3_3:83827539-83827558 Msa0479010:intron 50.0%
CAGTCATAGCAAGTGGCACA+AGG - chr3_3:83828014-83828033 None:intergenic 50.0%
GCATCCACCTCCTGAACAGA+AGG - chr3_3:83826826-83826845 None:intergenic 55.0%
GGACTGCCCTTACCTCTATG+TGG + chr3_3:83827485-83827504 Msa0479010:intron 55.0%
! GTCGCGAGAGTTTTCCACTG+TGG - chr3_3:83827514-83827533 None:intergenic 55.0%
CGCGACTCACAACCTTCTCT+TGG + chr3_3:83827528-83827547 Msa0479010:intron 55.0%
ACCGGTCACTACCCAAGAGA+AGG - chr3_3:83827543-83827562 None:intergenic 55.0%
CAGCGCCGCAAGTATAGGAG+TGG + chr3_3:83825698-83825717 Msa0479010:five_prime_UTR 60.0%
GGCTGCAATGGTGGCTGCAA+TGG + chr3_3:83825862-83825881 Msa0479010:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 83825458 83829851 83825458 ID=Msa0479010;Name=Msa0479010
chr3_3 mRNA 83825458 83829851 83825458 ID=Msa0479010-mRNA-1;Parent=Msa0479010;Name=Msa0479010-mRNA-1;_AED=0.27;_eAED=0.27;_QI=922|0.6|0.83|0.83|0.2|0.33|6|87|90
chr3_3 exon 83825458 83826238 83825458 ID=Msa0479010-mRNA-1:exon:23675;Parent=Msa0479010-mRNA-1
chr3_3 exon 83826679 83826954 83826679 ID=Msa0479010-mRNA-1:exon:23676;Parent=Msa0479010-mRNA-1
chr3_3 exon 83827260 83827349 83827260 ID=Msa0479010-mRNA-1:exon:23677;Parent=Msa0479010-mRNA-1
chr3_3 exon 83829484 83829497 83829484 ID=Msa0479010-mRNA-1:exon:23678;Parent=Msa0479010-mRNA-1
chr3_3 exon 83829593 83829685 83829593 ID=Msa0479010-mRNA-1:exon:23679;Parent=Msa0479010-mRNA-1
chr3_3 exon 83829824 83829851 83829824 ID=Msa0479010-mRNA-1:exon:23680;Parent=Msa0479010-mRNA-1
chr3_3 five_prime_UTR 83825458 83826238 83825458 ID=Msa0479010-mRNA-1:five_prime_utr;Parent=Msa0479010-mRNA-1
chr3_3 five_prime_UTR 83826679 83826819 83826679 ID=Msa0479010-mRNA-1:five_prime_utr;Parent=Msa0479010-mRNA-1
chr3_3 CDS 83826820 83826954 83826820 ID=Msa0479010-mRNA-1:cds;Parent=Msa0479010-mRNA-1
chr3_3 CDS 83827260 83827349 83827260 ID=Msa0479010-mRNA-1:cds;Parent=Msa0479010-mRNA-1
chr3_3 CDS 83829484 83829497 83829484 ID=Msa0479010-mRNA-1:cds;Parent=Msa0479010-mRNA-1
chr3_3 CDS 83829593 83829626 83829593 ID=Msa0479010-mRNA-1:cds;Parent=Msa0479010-mRNA-1
chr3_3 three_prime_UTR 83829627 83829685 83829627 ID=Msa0479010-mRNA-1:three_prime_utr;Parent=Msa0479010-mRNA-1
chr3_3 three_prime_UTR 83829824 83829851 83829824 ID=Msa0479010-mRNA-1:three_prime_utr;Parent=Msa0479010-mRNA-1
Gene Sequence

>Msa0479010

ATGCCTTCTGTTCAGGAGGTGGATGCAAAAGTTAAAGAGTTACGTCAAACTATTGCGAACCTGAACAATAAACAGATGTCATTGAGAAGTACTATAAAGAAATTGAAGGAGAGGACTGTGGAAATGGATGATAAGATTTCTGGTGCTGAATATAAACTGGTACAAAATGTTCAAGAGCATGGAAATCTACTTTCAAAAATTGCTCAATCGCCTGATAAAGTACAGGAGAGCTTATTGATATTCACTATACAGCTAGATTCGCCGATGGAATAG
Protein sequence

>Msa0479010

MPSVQEVDAKVKELRQTIANLNNKQMSLRSTIKKLKERTVEMDDKISGAEYKLVQNVQEHGNLLSKIAQSPDKVQESLLIFTIQLDSPME*