AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1196960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCACAAACATTAAATTT+AGG 0.206116 8_1:-47057982 None:intergenic
GAAGAAGCTGTGACTTTAAT+TGG 0.227175 8_1:+47057409 Msa1196960:five_prime_UTR
TCTTCAGCATAAGAAGTTTC+AGG 0.238833 8_1:-47057318 None:intergenic
CTTAATTTGACATGTTGTTT+TGG 0.249821 8_1:+47058071 Msa1196960:exon
ATGGTTTGAGACTCAAATTC+TGG 0.273976 8_1:-47057768 None:intergenic
TCTACACCCTTAGTAGATTC+AGG 0.310531 8_1:-47058337 None:intergenic
GCCATAGGTAAATGATTATA+AGG 0.310828 8_1:+47057121 Msa1196960:five_prime_UTR
CTCTACTAAGAGCTGAATAT+TGG 0.326378 8_1:-47057922 None:intergenic
TGTTATATGGTTGAAAGTTA+TGG 0.329251 8_1:+47058115 Msa1196960:CDS
ACTTTCTAGCACAGGTCATT+AGG 0.335552 8_1:-47057817 None:intergenic
TTTCCTAAATTTAATGTTTG+TGG 0.342398 8_1:+47057979 Msa1196960:five_prime_UTR
TACAAACAACTGACCTCTAT+TGG 0.359194 8_1:-47057454 None:intergenic
TGCAGAAAGCTCTGAAAATC+AGG 0.376100 8_1:+47058381 Msa1196960:CDS
CCCTTAGTAGATTCAGGAAT+AGG 0.380781 8_1:-47058331 None:intergenic
TTCTTATGCTGAAGATTGGT+TGG 0.389430 8_1:+47057326 Msa1196960:five_prime_UTR
TCGCTTCAATCAACCGTGTT+GGG 0.389841 8_1:+47057717 Msa1196960:five_prime_UTR
TAGTAGAGACCGCCAAGAAC+AGG 0.398173 8_1:+47057937 Msa1196960:five_prime_UTR
TCCTATTCCTGAATCTACTA+AGG 0.405787 8_1:+47058330 Msa1196960:CDS
TTAGGATCTCTTATGAAGTA+AGG 0.417246 8_1:-47057799 None:intergenic
TGGTTGAAAGTTATGGACTA+TGG 0.418645 8_1:+47058122 Msa1196960:CDS
AAACTTCTTATGCTGAAGAT+TGG 0.428394 8_1:+47057322 Msa1196960:five_prime_UTR
TTCAGAGCTTTCTGCAGCAT+TGG 0.431912 8_1:-47058374 None:intergenic
ATATCATGATTACATACAAT+TGG 0.432009 8_1:-47057147 None:intergenic
AATTAAAGTCACAGCTTCTT+CGG 0.433034 8_1:-47057407 None:intergenic
TGGGAATGGTTGATTATGCT+TGG 0.436255 8_1:+47057346 Msa1196960:five_prime_UTR
ACCTTATAATCATTTACCTA+TGG 0.440147 8_1:-47057122 None:intergenic
TCTTATGCTGAAGATTGGTT+GGG 0.449984 8_1:+47057327 Msa1196960:five_prime_UTR
CTTCAGCATAAGAAGTTTCA+GGG 0.461101 8_1:-47057317 None:intergenic
GTCGCTTCAATCAACCGTGT+TGG 0.479956 8_1:+47057716 Msa1196960:five_prime_UTR
CAAAATGTCCCTTCCCTACC+AGG 0.484387 8_1:-47058094 None:intergenic
TGCTGAAGATTGGTTGGGAA+TGG 0.521112 8_1:+47057332 Msa1196960:five_prime_UTR
CGAACCTATGTGGCCAATAG+AGG 0.523223 8_1:+47057441 Msa1196960:five_prime_UTR
ATAATCATTTACCTATGGCA+TGG 0.526574 8_1:-47057117 None:intergenic
TAATCATTTACCTATGGCAT+GGG 0.526799 8_1:-47057116 None:intergenic
ATGTATATTGCCCATGCCAT+AGG 0.539143 8_1:+47057106 Msa1196960:five_prime_UTR
CTGACCTCTATTGGCCACAT+AGG 0.542982 8_1:-47057445 None:intergenic
TAGGATCTCTTATGAAGTAA+GGG 0.546610 8_1:-47057798 None:intergenic
ATGAAGTAAGGGGTCACTGA+TGG 0.560614 8_1:-47057787 None:intergenic
CCTATTCCTGAATCTACTAA+GGG 0.574186 8_1:+47058331 Msa1196960:CDS
TAATGAACACTTTCTAGCAC+AGG 0.581116 8_1:-47057825 None:intergenic
AGTAGAGACCGCCAAGAACA+GGG 0.600506 8_1:+47057938 Msa1196960:five_prime_UTR
AGCTCATCAACAATGGCAGA+TGG 0.600942 8_1:+47058187 Msa1196960:CDS
TGATTTGAGCTCATCAACAA+TGG 0.616168 8_1:+47058180 Msa1196960:CDS
GTGAACTCAGCGAACCTATG+TGG 0.624704 8_1:+47057431 Msa1196960:five_prime_UTR
TGGTCTTTCTTCCTTCACCA+AGG 0.629656 8_1:+47058207 Msa1196960:CDS
AGGATCTCTTATGAAGTAAG+GGG 0.638589 8_1:-47057797 None:intergenic
AAGATGTCTCAGTCCCAACA+CGG 0.686028 8_1:-47057730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAATGCTTTTATTTTT+TGG + chr8_1:47057282-47057301 Msa1196960:five_prime_UTR 10.0%
!!! AAATAATGCTTTTATTTTTT+GGG + chr8_1:47057283-47057302 Msa1196960:five_prime_UTR 10.0%
!!! AAAATAATGCTTTTATTTTT+TGG + chr8_1:47057282-47057301 Msa1196960:five_prime_UTR 10.0%
!!! AAATAATGCTTTTATTTTTT+GGG + chr8_1:47057283-47057302 Msa1196960:five_prime_UTR 10.0%
!! ACTAAAAACAATAAGAAAAT+TGG + chr8_1:47057623-47057642 Msa1196960:intron 15.0%
!! ACTAAAAACAATAAGAAAAT+TGG + chr8_1:47057623-47057642 Msa1196960:intron 15.0%
!! ATATCATGATTACATACAAT+TGG - chr8_1:47057150-47057169 None:intergenic 20.0%
!! TTTCCTAAATTTAATGTTTG+TGG + chr8_1:47057979-47057998 Msa1196960:five_prime_UTR 20.0%
!! AAACCACAAACATTAAATTT+AGG - chr8_1:47057985-47058004 None:intergenic 20.0%
!!! TTTTAATTTTGATCATGTTG+AGG + chr8_1:47058002-47058021 Msa1196960:five_prime_UTR 20.0%
!!! ACATAGAATGTTGAAAATTT+TGG + chr8_1:47058896-47058915 Msa1196960:intron 20.0%
!! CAACCATATATTCTATAAAT+GGG - chr8_1:47058947-47058966 None:intergenic 20.0%
!! ATATCATGATTACATACAAT+TGG - chr8_1:47057150-47057169 None:intergenic 20.0%
!! TTTCCTAAATTTAATGTTTG+TGG + chr8_1:47057979-47057998 Msa1196960:five_prime_UTR 20.0%
!! AAACCACAAACATTAAATTT+AGG - chr8_1:47057985-47058004 None:intergenic 20.0%
!!! TTTTAATTTTGATCATGTTG+AGG + chr8_1:47058002-47058021 Msa1196960:five_prime_UTR 20.0%
!!! ACATAGAATGTTGAAAATTT+TGG + chr8_1:47058896-47058915 Msa1196960:intron 20.0%
!! CAACCATATATTCTATAAAT+GGG - chr8_1:47058947-47058966 None:intergenic 20.0%
! ACCTTATAATCATTTACCTA+TGG - chr8_1:47057125-47057144 None:intergenic 25.0%
! AGAAACAATAATCAGTAGAA+AGG - chr8_1:47057502-47057521 None:intergenic 25.0%
!! TATTTTATGCTTATACCTAC+AGG + chr8_1:47057568-47057587 Msa1196960:intron 25.0%
!! AGAAAATTGGCATGATTTTA+TGG + chr8_1:47057636-47057655 Msa1196960:intron 25.0%
!!! TATAGGTGTTATTGTTTTTC+AGG + chr8_1:47057661-47057680 Msa1196960:intron 25.0%
!!! CTTAATTTGACATGTTGTTT+TGG + chr8_1:47058071-47058090 Msa1196960:exon 25.0%
! TGTTATATGGTTGAAAGTTA+TGG + chr8_1:47058115-47058134 Msa1196960:CDS 25.0%
! ACACAGATTATTAATACAGA+AGG - chr8_1:47058261-47058280 None:intergenic 25.0%
! TAATCATGAAACTAGCTATA+TGG + chr8_1:47058616-47058635 Msa1196960:intron 25.0%
! ATGTACAAACTGAACTAAAT+AGG - chr8_1:47058880-47058899 None:intergenic 25.0%
! GCAACCATATATTCTATAAA+TGG - chr8_1:47058948-47058967 None:intergenic 25.0%
! ACCTTATAATCATTTACCTA+TGG - chr8_1:47057125-47057144 None:intergenic 25.0%
! AGAAACAATAATCAGTAGAA+AGG - chr8_1:47057502-47057521 None:intergenic 25.0%
!! TATTTTATGCTTATACCTAC+AGG + chr8_1:47057568-47057587 Msa1196960:intron 25.0%
!! AGAAAATTGGCATGATTTTA+TGG + chr8_1:47057636-47057655 Msa1196960:intron 25.0%
!!! TATAGGTGTTATTGTTTTTC+AGG + chr8_1:47057661-47057680 Msa1196960:intron 25.0%
!!! CTTAATTTGACATGTTGTTT+TGG + chr8_1:47058071-47058090 Msa1196960:exon 25.0%
! TGTTATATGGTTGAAAGTTA+TGG + chr8_1:47058115-47058134 Msa1196960:CDS 25.0%
! ACACAGATTATTAATACAGA+AGG - chr8_1:47058261-47058280 None:intergenic 25.0%
! TAATCATGAAACTAGCTATA+TGG + chr8_1:47058616-47058635 Msa1196960:intron 25.0%
! ATGTACAAACTGAACTAAAT+AGG - chr8_1:47058880-47058899 None:intergenic 25.0%
! GCAACCATATATTCTATAAA+TGG - chr8_1:47058948-47058967 None:intergenic 25.0%
TAATCATTTACCTATGGCAT+GGG - chr8_1:47057119-47057138 None:intergenic 30.0%
ATAATCATTTACCTATGGCA+TGG - chr8_1:47057120-47057139 None:intergenic 30.0%
GCCATAGGTAAATGATTATA+AGG + chr8_1:47057121-47057140 Msa1196960:five_prime_UTR 30.0%
AAACTTCTTATGCTGAAGAT+TGG + chr8_1:47057322-47057341 Msa1196960:five_prime_UTR 30.0%
AATTAAAGTCACAGCTTCTT+CGG - chr8_1:47057410-47057429 None:intergenic 30.0%
AGGTCAGTTGTTTGTATTAA+TGG + chr8_1:47057461-47057480 Msa1196960:intron 30.0%
!! ATTGTTTCTGATGCTTAGAT+TGG + chr8_1:47057513-47057532 Msa1196960:intron 30.0%
TAGGATCTCTTATGAAGTAA+GGG - chr8_1:47057801-47057820 None:intergenic 30.0%
TTAGGATCTCTTATGAAGTA+AGG - chr8_1:47057802-47057821 None:intergenic 30.0%
ATACAGAAGGAAAGTGAAAA+AGG - chr8_1:47058248-47058267 None:intergenic 30.0%
! CTCTCTTGTGTGTTTTATAA+TGG + chr8_1:47058663-47058682 Msa1196960:intron 30.0%
ACATAGAGAATAAGCTATTG+AGG + chr8_1:47058739-47058758 Msa1196960:intron 30.0%
TGACTGAATGTTGAAAACTT+TGG + chr8_1:47058844-47058863 Msa1196960:intron 30.0%
GGTCCCATTTATAGAATATA+TGG + chr8_1:47058941-47058960 Msa1196960:intron 30.0%
!! TTCCATTTTTCTATCTTCAG+TGG + chr8_1:47058977-47058996 Msa1196960:intron 30.0%
TAATCATTTACCTATGGCAT+GGG - chr8_1:47057119-47057138 None:intergenic 30.0%
ATAATCATTTACCTATGGCA+TGG - chr8_1:47057120-47057139 None:intergenic 30.0%
GCCATAGGTAAATGATTATA+AGG + chr8_1:47057121-47057140 Msa1196960:five_prime_UTR 30.0%
AAACTTCTTATGCTGAAGAT+TGG + chr8_1:47057322-47057341 Msa1196960:five_prime_UTR 30.0%
AATTAAAGTCACAGCTTCTT+CGG - chr8_1:47057410-47057429 None:intergenic 30.0%
AGGTCAGTTGTTTGTATTAA+TGG + chr8_1:47057461-47057480 Msa1196960:intron 30.0%
!! ATTGTTTCTGATGCTTAGAT+TGG + chr8_1:47057513-47057532 Msa1196960:intron 30.0%
TAGGATCTCTTATGAAGTAA+GGG - chr8_1:47057801-47057820 None:intergenic 30.0%
TTAGGATCTCTTATGAAGTA+AGG - chr8_1:47057802-47057821 None:intergenic 30.0%
ATACAGAAGGAAAGTGAAAA+AGG - chr8_1:47058248-47058267 None:intergenic 30.0%
! CTCTCTTGTGTGTTTTATAA+TGG + chr8_1:47058663-47058682 Msa1196960:intron 30.0%
ACATAGAGAATAAGCTATTG+AGG + chr8_1:47058739-47058758 Msa1196960:intron 30.0%
TGACTGAATGTTGAAAACTT+TGG + chr8_1:47058844-47058863 Msa1196960:intron 30.0%
GGTCCCATTTATAGAATATA+TGG + chr8_1:47058941-47058960 Msa1196960:intron 30.0%
!! TTCCATTTTTCTATCTTCAG+TGG + chr8_1:47058977-47058996 Msa1196960:intron 30.0%
!!! CTTTTATTTTTTGGGCAGCT+GGG + chr8_1:47057291-47057310 Msa1196960:five_prime_UTR 35.0%
CTTCAGCATAAGAAGTTTCA+GGG - chr8_1:47057320-47057339 None:intergenic 35.0%
TCTTCAGCATAAGAAGTTTC+AGG - chr8_1:47057321-47057340 None:intergenic 35.0%
TTCTTATGCTGAAGATTGGT+TGG + chr8_1:47057326-47057345 Msa1196960:five_prime_UTR 35.0%
! TCTTATGCTGAAGATTGGTT+GGG + chr8_1:47057327-47057346 Msa1196960:five_prime_UTR 35.0%
!! GCTTCTTCGGATTGTTTTAT+AGG - chr8_1:47057397-47057416 None:intergenic 35.0%
GAAGAAGCTGTGACTTTAAT+TGG + chr8_1:47057409-47057428 Msa1196960:five_prime_UTR 35.0%
TACAAACAACTGACCTCTAT+TGG - chr8_1:47057457-47057476 None:intergenic 35.0%
! GGCATGATTTTATGGCTTAT+AGG + chr8_1:47057644-47057663 Msa1196960:intron 35.0%
! ATGGTTTGAGACTCAAATTC+TGG - chr8_1:47057771-47057790 None:intergenic 35.0%
AGGATCTCTTATGAAGTAAG+GGG - chr8_1:47057800-47057819 None:intergenic 35.0%
! TAATGAACACTTTCTAGCAC+AGG - chr8_1:47057828-47057847 None:intergenic 35.0%
! TTCTCAAACTGTTTTCTGTG+TGG + chr8_1:47057854-47057873 Msa1196960:five_prime_UTR 35.0%
CTCTACTAAGAGCTGAATAT+TGG - chr8_1:47057925-47057944 None:intergenic 35.0%
! AAAGTGATTTTCCCTGTTCT+TGG - chr8_1:47057952-47057971 None:intergenic 35.0%
!! GGAAGGGACATTTTGTTATA+TGG + chr8_1:47058102-47058121 Msa1196960:CDS 35.0%
! TGGTTGAAAGTTATGGACTA+TGG + chr8_1:47058122-47058141 Msa1196960:CDS 35.0%
TGATTTGAGCTCATCAACAA+TGG + chr8_1:47058180-47058199 Msa1196960:CDS 35.0%
AAAAACACTAACCTTGGTGA+AGG - chr8_1:47058221-47058240 None:intergenic 35.0%
GGAAAGAAAAACACTAACCT+TGG - chr8_1:47058227-47058246 None:intergenic 35.0%
TCCTATTCCTGAATCTACTA+AGG + chr8_1:47058330-47058349 Msa1196960:CDS 35.0%
CCTATTCCTGAATCTACTAA+GGG + chr8_1:47058331-47058350 Msa1196960:CDS 35.0%
TACAGAAATTTAAATCCCCC+AGG - chr8_1:47058531-47058550 None:intergenic 35.0%
! GAATGTATTTGAAGTCCTGA+AGG + chr8_1:47058557-47058576 Msa1196960:intron 35.0%
ATGCAATTACTTGAACCTTC+AGG - chr8_1:47058575-47058594 None:intergenic 35.0%
GGTTCAAGTAATTGCATAAG+TGG + chr8_1:47058578-47058597 Msa1196960:intron 35.0%
GAACTCTATCATCACACATT+TGG - chr8_1:47058787-47058806 None:intergenic 35.0%
AATATAGAATGACCCAACAC+TGG + chr8_1:47058919-47058938 Msa1196960:intron 35.0%
ATATAGAATGACCCAACACT+GGG + chr8_1:47058920-47058939 Msa1196960:intron 35.0%
TGCCACTGAAGATAGAAAAA+TGG - chr8_1:47058982-47059001 None:intergenic 35.0%
!!! CTTTTATTTTTTGGGCAGCT+GGG + chr8_1:47057291-47057310 Msa1196960:five_prime_UTR 35.0%
CTTCAGCATAAGAAGTTTCA+GGG - chr8_1:47057320-47057339 None:intergenic 35.0%
TCTTCAGCATAAGAAGTTTC+AGG - chr8_1:47057321-47057340 None:intergenic 35.0%
TTCTTATGCTGAAGATTGGT+TGG + chr8_1:47057326-47057345 Msa1196960:five_prime_UTR 35.0%
! TCTTATGCTGAAGATTGGTT+GGG + chr8_1:47057327-47057346 Msa1196960:five_prime_UTR 35.0%
!! GCTTCTTCGGATTGTTTTAT+AGG - chr8_1:47057397-47057416 None:intergenic 35.0%
GAAGAAGCTGTGACTTTAAT+TGG + chr8_1:47057409-47057428 Msa1196960:five_prime_UTR 35.0%
TACAAACAACTGACCTCTAT+TGG - chr8_1:47057457-47057476 None:intergenic 35.0%
! GGCATGATTTTATGGCTTAT+AGG + chr8_1:47057644-47057663 Msa1196960:intron 35.0%
! ATGGTTTGAGACTCAAATTC+TGG - chr8_1:47057771-47057790 None:intergenic 35.0%
AGGATCTCTTATGAAGTAAG+GGG - chr8_1:47057800-47057819 None:intergenic 35.0%
! TAATGAACACTTTCTAGCAC+AGG - chr8_1:47057828-47057847 None:intergenic 35.0%
! TTCTCAAACTGTTTTCTGTG+TGG + chr8_1:47057854-47057873 Msa1196960:five_prime_UTR 35.0%
CTCTACTAAGAGCTGAATAT+TGG - chr8_1:47057925-47057944 None:intergenic 35.0%
! AAAGTGATTTTCCCTGTTCT+TGG - chr8_1:47057952-47057971 None:intergenic 35.0%
!! GGAAGGGACATTTTGTTATA+TGG + chr8_1:47058102-47058121 Msa1196960:CDS 35.0%
! TGGTTGAAAGTTATGGACTA+TGG + chr8_1:47058122-47058141 Msa1196960:CDS 35.0%
TGATTTGAGCTCATCAACAA+TGG + chr8_1:47058180-47058199 Msa1196960:CDS 35.0%
AAAAACACTAACCTTGGTGA+AGG - chr8_1:47058221-47058240 None:intergenic 35.0%
GGAAAGAAAAACACTAACCT+TGG - chr8_1:47058227-47058246 None:intergenic 35.0%
TCCTATTCCTGAATCTACTA+AGG + chr8_1:47058330-47058349 Msa1196960:CDS 35.0%
CCTATTCCTGAATCTACTAA+GGG + chr8_1:47058331-47058350 Msa1196960:CDS 35.0%
TACAGAAATTTAAATCCCCC+AGG - chr8_1:47058531-47058550 None:intergenic 35.0%
! GAATGTATTTGAAGTCCTGA+AGG + chr8_1:47058557-47058576 Msa1196960:intron 35.0%
ATGCAATTACTTGAACCTTC+AGG - chr8_1:47058575-47058594 None:intergenic 35.0%
GGTTCAAGTAATTGCATAAG+TGG + chr8_1:47058578-47058597 Msa1196960:intron 35.0%
GAACTCTATCATCACACATT+TGG - chr8_1:47058787-47058806 None:intergenic 35.0%
AATATAGAATGACCCAACAC+TGG + chr8_1:47058919-47058938 Msa1196960:intron 35.0%
ATATAGAATGACCCAACACT+GGG + chr8_1:47058920-47058939 Msa1196960:intron 35.0%
TGCCACTGAAGATAGAAAAA+TGG - chr8_1:47058982-47059001 None:intergenic 35.0%
ATGTATATTGCCCATGCCAT+AGG + chr8_1:47057106-47057125 Msa1196960:five_prime_UTR 40.0%
!!! GCTTTTATTTTTTGGGCAGC+TGG + chr8_1:47057290-47057309 Msa1196960:five_prime_UTR 40.0%
TGGGAATGGTTGATTATGCT+TGG + chr8_1:47057346-47057365 Msa1196960:five_prime_UTR 40.0%
! ACTTTCTAGCACAGGTCATT+AGG - chr8_1:47057820-47057839 None:intergenic 40.0%
!!! TTTGACATGTTGTTTTGGCC+TGG + chr8_1:47058076-47058095 Msa1196960:exon 40.0%
CCCTTAGTAGATTCAGGAAT+AGG - chr8_1:47058334-47058353 None:intergenic 40.0%
TCTACACCCTTAGTAGATTC+AGG - chr8_1:47058340-47058359 None:intergenic 40.0%
!! TGCAGAAAGCTCTGAAAATC+AGG + chr8_1:47058381-47058400 Msa1196960:CDS 40.0%
TATAAATGGGACCCAGTGTT+GGG - chr8_1:47058934-47058953 None:intergenic 40.0%
ATGTATATTGCCCATGCCAT+AGG + chr8_1:47057106-47057125 Msa1196960:five_prime_UTR 40.0%
!!! GCTTTTATTTTTTGGGCAGC+TGG + chr8_1:47057290-47057309 Msa1196960:five_prime_UTR 40.0%
TGGGAATGGTTGATTATGCT+TGG + chr8_1:47057346-47057365 Msa1196960:five_prime_UTR 40.0%
! ACTTTCTAGCACAGGTCATT+AGG - chr8_1:47057820-47057839 None:intergenic 40.0%
!!! TTTGACATGTTGTTTTGGCC+TGG + chr8_1:47058076-47058095 Msa1196960:exon 40.0%
CCCTTAGTAGATTCAGGAAT+AGG - chr8_1:47058334-47058353 None:intergenic 40.0%
TCTACACCCTTAGTAGATTC+AGG - chr8_1:47058340-47058359 None:intergenic 40.0%
!! TGCAGAAAGCTCTGAAAATC+AGG + chr8_1:47058381-47058400 Msa1196960:CDS 40.0%
TATAAATGGGACCCAGTGTT+GGG - chr8_1:47058934-47058953 None:intergenic 40.0%
! TGCTGAAGATTGGTTGGGAA+TGG + chr8_1:47057332-47057351 Msa1196960:five_prime_UTR 45.0%
TCGCTTCAATCAACCGTGTT+GGG + chr8_1:47057717-47057736 Msa1196960:five_prime_UTR 45.0%
AAGATGTCTCAGTCCCAACA+CGG - chr8_1:47057733-47057752 None:intergenic 45.0%
ATGAAGTAAGGGGTCACTGA+TGG - chr8_1:47057790-47057809 None:intergenic 45.0%
! GTGATTTTCCCTGTTCTTGG+CGG - chr8_1:47057949-47057968 None:intergenic 45.0%
!!! ACATGTTGTTTTGGCCTGGT+AGG + chr8_1:47058080-47058099 Msa1196960:exon 45.0%
!!! CATGTTGTTTTGGCCTGGTA+GGG + chr8_1:47058081-47058100 Msa1196960:exon 45.0%
AGCTCATCAACAATGGCAGA+TGG + chr8_1:47058187-47058206 Msa1196960:CDS 45.0%
TGGTCTTTCTTCCTTCACCA+AGG + chr8_1:47058207-47058226 Msa1196960:CDS 45.0%
GAGACGCTGATGAAGACTAA+AGG - chr8_1:47058306-47058325 None:intergenic 45.0%
TTCAGAGCTTTCTGCAGCAT+TGG - chr8_1:47058377-47058396 None:intergenic 45.0%
AATTATTGACATGCAGCCCC+TGG + chr8_1:47058478-47058497 Msa1196960:intron 45.0%
TTATGGTGCATAAGACGACC+TGG + chr8_1:47058510-47058529 Msa1196960:intron 45.0%
TATGGTGCATAAGACGACCT+GGG + chr8_1:47058511-47058530 Msa1196960:intron 45.0%
TGCAATAGCAGATGTGTTGC+AGG + chr8_1:47058693-47058712 Msa1196960:intron 45.0%
CTATAAATGGGACCCAGTGT+TGG - chr8_1:47058935-47058954 None:intergenic 45.0%
! TGCTGAAGATTGGTTGGGAA+TGG + chr8_1:47057332-47057351 Msa1196960:five_prime_UTR 45.0%
TCGCTTCAATCAACCGTGTT+GGG + chr8_1:47057717-47057736 Msa1196960:five_prime_UTR 45.0%
AAGATGTCTCAGTCCCAACA+CGG - chr8_1:47057733-47057752 None:intergenic 45.0%
ATGAAGTAAGGGGTCACTGA+TGG - chr8_1:47057790-47057809 None:intergenic 45.0%
! GTGATTTTCCCTGTTCTTGG+CGG - chr8_1:47057949-47057968 None:intergenic 45.0%
!!! ACATGTTGTTTTGGCCTGGT+AGG + chr8_1:47058080-47058099 Msa1196960:exon 45.0%
!!! CATGTTGTTTTGGCCTGGTA+GGG + chr8_1:47058081-47058100 Msa1196960:exon 45.0%
AGCTCATCAACAATGGCAGA+TGG + chr8_1:47058187-47058206 Msa1196960:CDS 45.0%
TGGTCTTTCTTCCTTCACCA+AGG + chr8_1:47058207-47058226 Msa1196960:CDS 45.0%
GAGACGCTGATGAAGACTAA+AGG - chr8_1:47058306-47058325 None:intergenic 45.0%
TTCAGAGCTTTCTGCAGCAT+TGG - chr8_1:47058377-47058396 None:intergenic 45.0%
AATTATTGACATGCAGCCCC+TGG + chr8_1:47058478-47058497 Msa1196960:intron 45.0%
TTATGGTGCATAAGACGACC+TGG + chr8_1:47058510-47058529 Msa1196960:intron 45.0%
TATGGTGCATAAGACGACCT+GGG + chr8_1:47058511-47058530 Msa1196960:intron 45.0%
TGCAATAGCAGATGTGTTGC+AGG + chr8_1:47058693-47058712 Msa1196960:intron 45.0%
CTATAAATGGGACCCAGTGT+TGG - chr8_1:47058935-47058954 None:intergenic 45.0%
GTGAACTCAGCGAACCTATG+TGG + chr8_1:47057431-47057450 Msa1196960:five_prime_UTR 50.0%
CGAACCTATGTGGCCAATAG+AGG + chr8_1:47057441-47057460 Msa1196960:five_prime_UTR 50.0%
CTGACCTCTATTGGCCACAT+AGG - chr8_1:47057448-47057467 None:intergenic 50.0%
CCAGCTGAATGTCAACCTGT+AGG - chr8_1:47057586-47057605 None:intergenic 50.0%
! CCTACAGGTTGACATTCAGC+TGG + chr8_1:47057583-47057602 Msa1196960:intron 50.0%
GTCGCTTCAATCAACCGTGT+TGG + chr8_1:47057716-47057735 Msa1196960:five_prime_UTR 50.0%
TAGTAGAGACCGCCAAGAAC+AGG + chr8_1:47057937-47057956 Msa1196960:five_prime_UTR 50.0%
AGTAGAGACCGCCAAGAACA+GGG + chr8_1:47057938-47057957 Msa1196960:five_prime_UTR 50.0%
!!! TTGTTTTGGCCTGGTAGGGA+AGG + chr8_1:47058085-47058104 Msa1196960:CDS 50.0%
!!! TGTTTTGGCCTGGTAGGGAA+GGG + chr8_1:47058086-47058105 Msa1196960:CDS 50.0%
CAAAATGTCCCTTCCCTACC+AGG - chr8_1:47058097-47058116 None:intergenic 50.0%
! CAGGTTTTGCATCTCTCTCC+CGG + chr8_1:47058400-47058419 Msa1196960:intron 50.0%
ACCATAAGCAGAGCTACCAG+GGG - chr8_1:47058497-47058516 None:intergenic 50.0%
CACCATAAGCAGAGCTACCA+GGG - chr8_1:47058498-47058517 None:intergenic 50.0%
ATGGTGCATAAGACGACCTG+GGG + chr8_1:47058512-47058531 Msa1196960:intron 50.0%
GTGAACTCAGCGAACCTATG+TGG + chr8_1:47057431-47057450 Msa1196960:five_prime_UTR 50.0%
CGAACCTATGTGGCCAATAG+AGG + chr8_1:47057441-47057460 Msa1196960:five_prime_UTR 50.0%
CTGACCTCTATTGGCCACAT+AGG - chr8_1:47057448-47057467 None:intergenic 50.0%
CCAGCTGAATGTCAACCTGT+AGG - chr8_1:47057586-47057605 None:intergenic 50.0%
! CCTACAGGTTGACATTCAGC+TGG + chr8_1:47057583-47057602 Msa1196960:intron 50.0%
GTCGCTTCAATCAACCGTGT+TGG + chr8_1:47057716-47057735 Msa1196960:five_prime_UTR 50.0%
TAGTAGAGACCGCCAAGAAC+AGG + chr8_1:47057937-47057956 Msa1196960:five_prime_UTR 50.0%
AGTAGAGACCGCCAAGAACA+GGG + chr8_1:47057938-47057957 Msa1196960:five_prime_UTR 50.0%
!!! TTGTTTTGGCCTGGTAGGGA+AGG + chr8_1:47058085-47058104 Msa1196960:CDS 50.0%
!!! TGTTTTGGCCTGGTAGGGAA+GGG + chr8_1:47058086-47058105 Msa1196960:CDS 50.0%
CAAAATGTCCCTTCCCTACC+AGG - chr8_1:47058097-47058116 None:intergenic 50.0%
! CAGGTTTTGCATCTCTCTCC+CGG + chr8_1:47058400-47058419 Msa1196960:intron 50.0%
ACCATAAGCAGAGCTACCAG+GGG - chr8_1:47058497-47058516 None:intergenic 50.0%
CACCATAAGCAGAGCTACCA+GGG - chr8_1:47058498-47058517 None:intergenic 50.0%
ATGGTGCATAAGACGACCTG+GGG + chr8_1:47058512-47058531 Msa1196960:intron 50.0%
GCACCATAAGCAGAGCTACC+AGG - chr8_1:47058499-47058518 None:intergenic 55.0%
TGGTGCATAAGACGACCTGG+GGG + chr8_1:47058513-47058532 Msa1196960:intron 55.0%
GCACCATAAGCAGAGCTACC+AGG - chr8_1:47058499-47058518 None:intergenic 55.0%
TGGTGCATAAGACGACCTGG+GGG + chr8_1:47058513-47058532 Msa1196960:intron 55.0%
! GCCCCTGGTAGCTCTGCTTA+TGG + chr8_1:47058493-47058512 Msa1196960:intron 60.0%
! GCCCCTGGTAGCTCTGCTTA+TGG + chr8_1:47058493-47058512 Msa1196960:intron 60.0%
GCGCGCTCGTTTGTGTGTCC+GGG - chr8_1:47058421-47058440 None:intergenic 65.0%
CGCGCGCTCGTTTGTGTGTC+CGG - chr8_1:47058422-47058441 None:intergenic 65.0%
GCGCGCTCGTTTGTGTGTCC+GGG - chr8_1:47058421-47058440 None:intergenic 65.0%
CGCGCGCTCGTTTGTGTGTC+CGG - chr8_1:47058422-47058441 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 47057101 47059054 47057101 ID=Msa1196960;Name=Msa1196960
chr8_1 mRNA 47057101 47059054 47057101 ID=Msa1196960-mRNA-1;Parent=Msa1196960;Name=Msa1196960-mRNA-1;_AED=0.26;_eAED=0.26;_QI=761|0.33|0.42|1|0.33|0.14|7|0|88
chr8_1 exon 47057101 47057462 47057101 ID=Msa1196960-mRNA-1:exon:14894;Parent=Msa1196960-mRNA-1
chr8_1 exon 47057683 47058228 47057683 ID=Msa1196960-mRNA-1:exon:14895;Parent=Msa1196960-mRNA-1
chr8_1 exon 47058310 47058402 47058310 ID=Msa1196960-mRNA-1:exon:14896;Parent=Msa1196960-mRNA-1
chr8_1 exon 47058494 47058499 47058494 ID=Msa1196960-mRNA-1:exon:14897;Parent=Msa1196960-mRNA-1
chr8_1 exon 47058579 47058584 47058579 ID=Msa1196960-mRNA-1:exon:14898;Parent=Msa1196960-mRNA-1
chr8_1 exon 47058997 47059002 47058997 ID=Msa1196960-mRNA-1:exon:14899;Parent=Msa1196960-mRNA-1
chr8_1 exon 47059049 47059054 47059049 ID=Msa1196960-mRNA-1:exon:14900;Parent=Msa1196960-mRNA-1
chr8_1 five_prime_UTR 47057101 47057462 47057101 ID=Msa1196960-mRNA-1:five_prime_utr;Parent=Msa1196960-mRNA-1
chr8_1 five_prime_UTR 47057683 47058081 47057683 ID=Msa1196960-mRNA-1:five_prime_utr;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47058082 47058228 47058082 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47058310 47058402 47058310 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47058494 47058499 47058494 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47058579 47058584 47058579 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47058997 47059002 47058997 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
chr8_1 CDS 47059049 47059054 47059049 ID=Msa1196960-mRNA-1:cds;Parent=Msa1196960-mRNA-1
Gene Sequence

>Msa1196960

ATGTTGTTTTGGCCTGGTAGGGAAGGGACATTTTGTTATATGGTTGAAAGTTATGGACTATGGAGACATGTGACAGAATCATTAAAACTATTCTCTTTTGATTTGAGCTCATCAACAATGGCAGATGGTCTTTCTTCCTTCACCAAGTCTTCATCAGCGTCTCAAAATCCTATTCCTGAATCTACTAAGGGTGTAGAAAATGTTAAAGATTCCAATGCTGCAGAAAGCTCTGAAAATCAGCCCCTGGTTCAATGGCATATTAGA
Protein sequence

>Msa1196960

MLFWPGREGTFCYMVESYGLWRHVTESLKLFSFDLSSSTMADGLSSFTKSSSASQNPIPESTKGVENVKDSNAAESSENQPLVQWHIR