AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0493500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493500 MtrunA17_Chr3g0087151 80.702 228 26 3 3 212 25 252 6.84e-93 272
Msa0493500 MtrunA17_Chr3g0087231 56.604 265 39 5 24 212 80 344 8.29e-57 183
Msa0493500 MtrunA17_Chr3g0087231 57.798 218 75 3 2 206 4 217 7.26e-52 170
Msa0493500 MtrunA17_Chr3g0087231 62.304 191 43 5 14 178 147 334 1.49e-40 141
Msa0493500 MtrunA17_Chr3g0087211 53.986 276 40 5 24 212 85 360 2.01e-56 182
Msa0493500 MtrunA17_Chr3g0087211 59.545 220 73 4 2 206 4 222 6.29e-54 176
Msa0493500 MtrunA17_Chr3g0087041 70.988 162 33 2 51 212 37 184 1.36e-50 162
Msa0493500 MtrunA17_Chr3g0087041 77.391 115 15 1 24 127 64 178 1.50e-37 129
Msa0493500 MtrunA17_Chr3g0087041 65.517 174 35 9 2 170 24 177 9.06e-36 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0493500 AT5G09530.1 60.870 207 52 5 24 205 44 246 4.76e-47 159
Msa0493500 AT5G09530.1 63.547 203 48 7 24 205 143 340 5.71e-44 151
Msa0493500 AT5G09530.1 60.094 213 46 8 27 206 102 308 5.09e-42 145
Msa0493500 AT5G09530.1 66.860 172 44 6 40 205 43 207 8.45e-40 140
Msa0493500 AT5G09530.1 57.746 213 60 9 15 204 165 370 2.28e-38 136
Msa0493500 AT5G09530.1 69.159 107 29 2 99 205 39 141 2.43e-24 99.0
Msa0493500 AT5G09520.1 56.881 109 35 3 96 204 34 130 7.74e-20 82.0
Msa0493500 AT5G09520.1 57.732 97 34 2 70 166 41 130 1.45e-17 75.9
Msa0493500 AT5G09520.1 55.814 86 34 1 131 212 34 119 2.33e-15 70.1
Msa0493500 AT5G09520.1 58.333 96 27 5 24 115 42 128 1.65e-14 68.2
Msa0493500 AT5G09520.1 58.025 81 24 3 26 98 52 130 3.38e-12 62.0

Find 74 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACCTTTGGTATTTC+TGG 0.080700 3_4:-18639823 None:intergenic
TCAGGTATCTTTGGAATTTC+TGG 0.086251 3_4:-18639619 None:intergenic
CCTATATCTGCATTGAAATT+TGG 0.142531 3_4:-18638918 None:intergenic
AGCTCAGGTTTGGGCAATTC+AGG 0.197717 3_4:-18639571 None:intergenic
GGAACATCTTTAGGAGTTTC+AGG 0.202854 3_4:-18639946 None:intergenic
AATTCAGGGGCATTAAATTT+AGG 0.233034 3_4:-18639661 None:intergenic
AACTCAGGCTTTGGCAATTC+AGG 0.237404 3_4:-18639637 None:intergenic
TTAGGAGTTTCAGGGAATTT+AGG 0.242346 3_4:-18639937 None:intergenic
AATTCTTGCTTTGGCAATTC+AGG 0.243854 3_4:-18639430 None:intergenic
AACTCTAGCTTTGGTAATTC+AGG 0.247874 3_4:-18639841 None:intergenic
ACCTTTGGTATTTCTGGTTT+GGG 0.248136 3_4:-18639817 None:intergenic
GACTTTAGGTAGCTCAGGTT+TGG 0.265600 3_4:-18639581 None:intergenic
TACCTTTGGTATTTCTGGTT+TGG 0.269930 3_4:-18639818 None:intergenic
ACTTTAGGTTGCTCGGGTTT+TGG 0.275487 3_4:-18639685 None:intergenic
CCAAATTTCAATGCAGATAT+AGG 0.276175 3_4:+18638918 Msa0493500:CDS
TTTGGCAATTCAGGGACTTT+AGG 0.288403 3_4:-18639700 None:intergenic
TTGGGCAACTCAGGGACTTT+TGG 0.292556 3_4:-18639799 None:intergenic
TCAGGTACCTTAGGGATATC+TGG 0.309626 3_4:-18639757 None:intergenic
ATAGACAATTCGGGGACTTT+AGG 0.311738 3_4:-18639595 None:intergenic
AGCTCAGGCTTTGGAAGTTC+AGG 0.319717 3_4:-18639463 None:intergenic
AATTTAGGTAACTCGGGTTT+TGG 0.322053 3_4:-18639922 None:intergenic
TTTGGCAATTCAGGTATCTT+TGG 0.328199 3_4:-18639628 None:intergenic
GTATTGGTTGGAACATCTTT+AGG 0.328990 3_4:-18639955 None:intergenic
TTAGGCAACTCGGGAACTTT+AGG 0.329308 3_4:-18639544 None:intergenic
TACCTTAGGGATATCTGGTT+TGG 0.332667 3_4:-18639752 None:intergenic
ACTTTGGGAAACTCGGGTTT+TGG 0.341260 3_4:-18639511 None:intergenic
ACTTTAGGTAGTTCATGTTT+TGG 0.353892 3_4:-18639718 None:intergenic
TTTGGTAACTCAGGTACCTT+AGG 0.357882 3_4:-18639766 None:intergenic
TTTAGGCAACTCGGGAACTT+TGG 0.361526 3_4:-18639527 None:intergenic
TTAGGCAACTCGGGAACTTT+GGG 0.361579 3_4:-18639526 None:intergenic
TTGGGCAACTCAGAGACTTT+AGG 0.363342 3_4:-18639733 None:intergenic
ACTTTAGGTAGCTCAGGTTT+GGG 0.365399 3_4:-18639580 None:intergenic
AATTTCTGGCATAGACAATT+CGG 0.365865 3_4:-18639605 None:intergenic
ACCTTAGGTAATTCTTGCTT+TGG 0.366481 3_4:-18639439 None:intergenic
ACATTAACTTTAGGTAGTTC+AGG 0.370017 3_4:-18639889 None:intergenic
TTCAGGGACTTTAGGTTGCT+CGG 0.381939 3_4:-18639692 None:intergenic
TTACGCTTCCATGGTTCAAT+TGG 0.383851 3_4:+18638860 None:intergenic
AGTTCAGGGACATTAACTTT+AGG 0.387475 3_4:-18639898 None:intergenic
CTCGGGAACTTTGGGAAACT+CGG 0.388765 3_4:-18639518 None:intergenic
AATTTAGGTAACTCAGGCTT+TGG 0.391757 3_4:-18639646 None:intergenic
ATTTCTGGCATAGACAATTC+GGG 0.397522 3_4:-18639604 None:intergenic
ATTTCTGGTTTGGGCAACTC+AGG 0.399272 3_4:-18639808 None:intergenic
TTTGGTAATTCAGGTACCTT+TGG 0.399783 3_4:-18639832 None:intergenic
AACTTAGGTAGCTCAGGCTT+TGG 0.406351 3_4:-18639472 None:intergenic
TTTGGAAGTTCAGGTACCTT+AGG 0.422522 3_4:-18639454 None:intergenic
ACCTTAGGGATATCTGGTTT+GGG 0.423518 3_4:-18639751 None:intergenic
ATCTTAGGTAACTCTAGCTT+TGG 0.432161 3_4:-18639850 None:intergenic
AGTTCCAACTTAAGGAGTAT+TGG 0.438613 3_4:-18639971 None:intergenic
TTTGGCAATTCAGGTACCTT+AGG 0.458454 3_4:-18639421 None:intergenic
ACATTAAACTTAGGTAGCTC+AGG 0.466667 3_4:-18639478 None:intergenic
TCGGGGACTTTAGGTAGCTC+AGG 0.467048 3_4:-18639586 None:intergenic
TTGGGCAATTCAGGGAACTT+AGG 0.475381 3_4:-18639562 None:intergenic
TTCAGGGAATTTAGGTAACT+CGG 0.478958 3_4:-18639929 None:intergenic
CTCGGGAACTTTAGGCAACT+CGG 0.481382 3_4:-18639536 None:intergenic
AATTCAGGAACATTAAACTT+AGG 0.490699 3_4:-18639487 None:intergenic
GAACATCTTTAGGAGTTTCA+GGG 0.495266 3_4:-18639945 None:intergenic
TTCAGGGAACTTAGGCAACT+CGG 0.499436 3_4:-18639554 None:intergenic
TTGGTAACTCAGGTACCTTA+GGG 0.506353 3_4:-18639765 None:intergenic
TCAGGGACTTTAGGTTGCTC+GGG 0.512283 3_4:-18639691 None:intergenic
GCATTAAATTTAGGTAACTC+AGG 0.520808 3_4:-18639652 None:intergenic
TCGGGAACTTTGGGAAACTC+GGG 0.521735 3_4:-18639517 None:intergenic
GCTCAGGTTTGGGCAATTCA+GGG 0.523757 3_4:-18639570 None:intergenic
CCAACTTAAGGAGTATTGGT+TGG 0.525013 3_4:-18639967 None:intergenic
TTTCTGGTTTGGGCAACTCA+GGG 0.529305 3_4:-18639807 None:intergenic
TATCTGCATTGAAATTTGGA+AGG 0.536393 3_4:-18638914 None:intergenic
TCAGGGAATTTAGGTAACTC+GGG 0.549724 3_4:-18639928 None:intergenic
TCGGGAACTTTAGGCAACTC+GGG 0.569457 3_4:-18639535 None:intergenic
TCAGGGAACTTAGGCAACTC+GGG 0.582113 3_4:-18639553 None:intergenic
GCCAAAGCAAGAATTACCTA+AGG 0.602511 3_4:+18639438 Msa0493500:CDS
TTTCTGGCATAGACAATTCG+GGG 0.611978 3_4:-18639603 None:intergenic
GCCTAAGCCAGAATTGCCTA+AGG 0.613699 3_4:+18639405 Msa0493500:intron
GCCCAAACCAGATATCCCTA+AGG 0.649847 3_4:+18639750 Msa0493500:CDS
TCTCAAAGCCAATTGAACCA+TGG 0.662039 3_4:-18638868 None:intergenic
GCCCAAACCAGAAATACCAA+AGG 0.685777 3_4:+18639816 Msa0493500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCTAATTAAATTTTGTAT+AGG + chr3_4:18638993-18639012 Msa0493500:intron 15.0%
!!! GTTCTAATTAAATTTTGTAT+AGG + chr3_4:18638993-18639012 Msa0493500:intron 15.0%
!! AAAAAATAGAACGTTATTGT+TGG - chr3_4:18638952-18638971 None:intergenic 20.0%
!! AAAAAATAGAACGTTATTGT+TGG - chr3_4:18638952-18638971 None:intergenic 20.0%
! CTAAAAAAATGTGTAAGCAA+GGG - chr3_4:18639068-18639087 None:intergenic 25.0%
! ACTAAAAAAATGTGTAAGCA+AGG - chr3_4:18639069-18639088 None:intergenic 25.0%
! TTCAGGAAGATATATTATAG+TGG - chr3_4:18639180-18639199 None:intergenic 25.0%
! AGTATAGCTAGAATGATTAA+TGG - chr3_4:18639262-18639281 None:intergenic 25.0%
! AATTCAGGAACATTAAACTT+AGG - chr3_4:18639490-18639509 None:intergenic 25.0%
!!! TTTTTCAATTCAGGTATCTT+AGG - chr3_4:18639868-18639887 None:intergenic 25.0%
! CTAAAAAAATGTGTAAGCAA+GGG - chr3_4:18639068-18639087 None:intergenic 25.0%
! ACTAAAAAAATGTGTAAGCA+AGG - chr3_4:18639069-18639088 None:intergenic 25.0%
! TTCAGGAAGATATATTATAG+TGG - chr3_4:18639180-18639199 None:intergenic 25.0%
! AGTATAGCTAGAATGATTAA+TGG - chr3_4:18639262-18639281 None:intergenic 25.0%
! AATTCAGGAACATTAAACTT+AGG - chr3_4:18639490-18639509 None:intergenic 25.0%
!!! TTTTTCAATTCAGGTATCTT+AGG - chr3_4:18639868-18639887 None:intergenic 25.0%
TATCTGCATTGAAATTTGGA+AGG - chr3_4:18638917-18638936 None:intergenic 30.0%
CCTATATCTGCATTGAAATT+TGG - chr3_4:18638921-18638940 None:intergenic 30.0%
CCAAATTTCAATGCAGATAT+AGG + chr3_4:18638918-18638937 Msa0493500:CDS 30.0%
! TGTGTTTTGTATGTGTTGTA+TGG - chr3_4:18639117-18639136 None:intergenic 30.0%
!! AAGAGTATTACAATGGTTGT+CGG + chr3_4:18639289-18639308 Msa0493500:intron 30.0%
! ATAGGTGGAAAATCAAGTTT+TGG - chr3_4:18639352-18639371 None:intergenic 30.0%
!! AATTTCTGGCATAGACAATT+CGG - chr3_4:18639608-18639627 None:intergenic 30.0%
GCATTAAATTTAGGTAACTC+AGG - chr3_4:18639655-18639674 None:intergenic 30.0%
!! AATTCAGGGGCATTAAATTT+AGG - chr3_4:18639664-18639683 None:intergenic 30.0%
! ACTTTAGGTAGTTCATGTTT+TGG - chr3_4:18639721-18639740 None:intergenic 30.0%
ACATTAACTTTAGGTAGTTC+AGG - chr3_4:18639892-18639911 None:intergenic 30.0%
TATCTGCATTGAAATTTGGA+AGG - chr3_4:18638917-18638936 None:intergenic 30.0%
CCTATATCTGCATTGAAATT+TGG - chr3_4:18638921-18638940 None:intergenic 30.0%
CCAAATTTCAATGCAGATAT+AGG + chr3_4:18638918-18638937 Msa0493500:CDS 30.0%
! TGTGTTTTGTATGTGTTGTA+TGG - chr3_4:18639117-18639136 None:intergenic 30.0%
!! AAGAGTATTACAATGGTTGT+CGG + chr3_4:18639289-18639308 Msa0493500:intron 30.0%
! ATAGGTGGAAAATCAAGTTT+TGG - chr3_4:18639352-18639371 None:intergenic 30.0%
!! AATTTCTGGCATAGACAATT+CGG - chr3_4:18639608-18639627 None:intergenic 30.0%
GCATTAAATTTAGGTAACTC+AGG - chr3_4:18639655-18639674 None:intergenic 30.0%
!! AATTCAGGGGCATTAAATTT+AGG - chr3_4:18639664-18639683 None:intergenic 30.0%
! ACTTTAGGTAGTTCATGTTT+TGG - chr3_4:18639721-18639740 None:intergenic 30.0%
ACATTAACTTTAGGTAGTTC+AGG - chr3_4:18639892-18639911 None:intergenic 30.0%
! GTTTTGTATGTGTTGTATGG+AGG - chr3_4:18639114-18639133 None:intergenic 35.0%
TGAGAAATTGATGTAAGCCA+TGG - chr3_4:18639227-18639246 None:intergenic 35.0%
GACAACCATTGTAATACTCT+TGG - chr3_4:18639290-18639309 None:intergenic 35.0%
! AGAGTATTACAATGGTTGTC+GGG + chr3_4:18639290-18639309 Msa0493500:intron 35.0%
!!! AAATCAAGTTTTGGAACCTC+AGG - chr3_4:18639343-18639362 None:intergenic 35.0%
TTAGGCAATTCAGGAATCTT+AGG - chr3_4:18639391-18639410 None:intergenic 35.0%
AATTCTTGCTTTGGCAATTC+AGG - chr3_4:18639433-18639452 None:intergenic 35.0%
ACCTTAGGTAATTCTTGCTT+TGG - chr3_4:18639442-18639461 None:intergenic 35.0%
ACATTAAACTTAGGTAGCTC+AGG - chr3_4:18639481-18639500 None:intergenic 35.0%
!! ATTTCTGGCATAGACAATTC+GGG - chr3_4:18639607-18639626 None:intergenic 35.0%
!! TCAGGTATCTTTGGAATTTC+TGG - chr3_4:18639622-18639641 None:intergenic 35.0%
TTTGGCAATTCAGGTATCTT+TGG - chr3_4:18639631-18639650 None:intergenic 35.0%
AATTTAGGTAACTCAGGCTT+TGG - chr3_4:18639649-18639668 None:intergenic 35.0%
!!! GTTCATGTTTTGGCAATTCA+GGG - chr3_4:18639711-18639730 None:intergenic 35.0%
!!! AGTTCATGTTTTGGCAATTC+AGG - chr3_4:18639712-18639731 None:intergenic 35.0%
!! ACCTTTGGTATTTCTGGTTT+GGG - chr3_4:18639820-18639839 None:intergenic 35.0%
!! TACCTTTGGTATTTCTGGTT+TGG - chr3_4:18639821-18639840 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+TGG - chr3_4:18639835-18639854 None:intergenic 35.0%
!! AACTCTAGCTTTGGTAATTC+AGG - chr3_4:18639844-18639863 None:intergenic 35.0%
! ATCTTAGGTAACTCTAGCTT+TGG - chr3_4:18639853-18639872 None:intergenic 35.0%
!!! AGTTCAGGCTTTTTCAATTC+AGG - chr3_4:18639877-18639896 None:intergenic 35.0%
AGTTCAGGGACATTAACTTT+AGG - chr3_4:18639901-18639920 None:intergenic 35.0%
! AATTTAGGTAACTCGGGTTT+TGG - chr3_4:18639925-18639944 None:intergenic 35.0%
TTCAGGGAATTTAGGTAACT+CGG - chr3_4:18639932-18639951 None:intergenic 35.0%
TTAGGAGTTTCAGGGAATTT+AGG - chr3_4:18639940-18639959 None:intergenic 35.0%
! GAACATCTTTAGGAGTTTCA+GGG - chr3_4:18639948-18639967 None:intergenic 35.0%
!! GTATTGGTTGGAACATCTTT+AGG - chr3_4:18639958-18639977 None:intergenic 35.0%
! GTTTTGTATGTGTTGTATGG+AGG - chr3_4:18639114-18639133 None:intergenic 35.0%
TGAGAAATTGATGTAAGCCA+TGG - chr3_4:18639227-18639246 None:intergenic 35.0%
GACAACCATTGTAATACTCT+TGG - chr3_4:18639290-18639309 None:intergenic 35.0%
! AGAGTATTACAATGGTTGTC+GGG + chr3_4:18639290-18639309 Msa0493500:intron 35.0%
!!! AAATCAAGTTTTGGAACCTC+AGG - chr3_4:18639343-18639362 None:intergenic 35.0%
TTAGGCAATTCAGGAATCTT+AGG - chr3_4:18639391-18639410 None:intergenic 35.0%
AATTCTTGCTTTGGCAATTC+AGG - chr3_4:18639433-18639452 None:intergenic 35.0%
ACCTTAGGTAATTCTTGCTT+TGG - chr3_4:18639442-18639461 None:intergenic 35.0%
ACATTAAACTTAGGTAGCTC+AGG - chr3_4:18639481-18639500 None:intergenic 35.0%
!! ATTTCTGGCATAGACAATTC+GGG - chr3_4:18639607-18639626 None:intergenic 35.0%
!! TCAGGTATCTTTGGAATTTC+TGG - chr3_4:18639622-18639641 None:intergenic 35.0%
TTTGGCAATTCAGGTATCTT+TGG - chr3_4:18639631-18639650 None:intergenic 35.0%
AATTTAGGTAACTCAGGCTT+TGG - chr3_4:18639649-18639668 None:intergenic 35.0%
!!! GTTCATGTTTTGGCAATTCA+GGG - chr3_4:18639711-18639730 None:intergenic 35.0%
!!! AGTTCATGTTTTGGCAATTC+AGG - chr3_4:18639712-18639731 None:intergenic 35.0%
!! ACCTTTGGTATTTCTGGTTT+GGG - chr3_4:18639820-18639839 None:intergenic 35.0%
!! TACCTTTGGTATTTCTGGTT+TGG - chr3_4:18639821-18639840 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+TGG - chr3_4:18639835-18639854 None:intergenic 35.0%
!! AACTCTAGCTTTGGTAATTC+AGG - chr3_4:18639844-18639863 None:intergenic 35.0%
! ATCTTAGGTAACTCTAGCTT+TGG - chr3_4:18639853-18639872 None:intergenic 35.0%
!!! AGTTCAGGCTTTTTCAATTC+AGG - chr3_4:18639877-18639896 None:intergenic 35.0%
AGTTCAGGGACATTAACTTT+AGG - chr3_4:18639901-18639920 None:intergenic 35.0%
! AATTTAGGTAACTCGGGTTT+TGG - chr3_4:18639925-18639944 None:intergenic 35.0%
TTCAGGGAATTTAGGTAACT+CGG - chr3_4:18639932-18639951 None:intergenic 35.0%
TTAGGAGTTTCAGGGAATTT+AGG - chr3_4:18639940-18639959 None:intergenic 35.0%
! GAACATCTTTAGGAGTTTCA+GGG - chr3_4:18639948-18639967 None:intergenic 35.0%
!! GTATTGGTTGGAACATCTTT+AGG - chr3_4:18639958-18639977 None:intergenic 35.0%
ATGTGTAAGCAAGGGTAAGT+TGG - chr3_4:18639060-18639079 None:intergenic 40.0%
AAGCAGCTGAGAAAAACTTC+AGG - chr3_4:18639197-18639216 None:intergenic 40.0%
TCAGCTGCTTGATTAAACCA+TGG + chr3_4:18639207-18639226 Msa0493500:intron 40.0%
!! GTCAGCCAAGAGTATTACAA+TGG + chr3_4:18639282-18639301 Msa0493500:intron 40.0%
GAGTATTACAATGGTTGTCG+GGG + chr3_4:18639291-18639310 Msa0493500:intron 40.0%
ATCTTAGGAAGCTAAGGCTT+TGG - chr3_4:18639376-18639395 None:intergenic 40.0%
TCAGGAATCTTAGGAAGCTA+AGG - chr3_4:18639382-18639401 None:intergenic 40.0%
!! AATTCTGGCTTAGGCAATTC+AGG - chr3_4:18639400-18639419 None:intergenic 40.0%
TTTGGCAATTCAGGTACCTT+AGG - chr3_4:18639424-18639443 None:intergenic 40.0%
GCCAAAGCAAGAATTACCTA+AGG + chr3_4:18639438-18639457 Msa0493500:CDS 40.0%
TTTGGAAGTTCAGGTACCTT+AGG - chr3_4:18639457-18639476 None:intergenic 40.0%
ACTTTAGGTAGCTCAGGTTT+GGG - chr3_4:18639583-18639602 None:intergenic 40.0%
ATAGACAATTCGGGGACTTT+AGG - chr3_4:18639598-18639617 None:intergenic 40.0%
! TTTCTGGCATAGACAATTCG+GGG - chr3_4:18639606-18639625 None:intergenic 40.0%
TTTGGCAATTCAGGGACTTT+AGG - chr3_4:18639703-18639722 None:intergenic 40.0%
! ACCTTAGGGATATCTGGTTT+GGG - chr3_4:18639754-18639773 None:intergenic 40.0%
TACCTTAGGGATATCTGGTT+TGG - chr3_4:18639755-18639774 None:intergenic 40.0%
! TTGGTAACTCAGGTACCTTA+GGG - chr3_4:18639768-18639787 None:intergenic 40.0%
! TTTGGTAACTCAGGTACCTT+AGG - chr3_4:18639769-18639788 None:intergenic 40.0%
TCAGGTACCTTTGGTATTTC+TGG - chr3_4:18639826-18639845 None:intergenic 40.0%
TCAGGGAATTTAGGTAACTC+GGG - chr3_4:18639931-18639950 None:intergenic 40.0%
! GGAACATCTTTAGGAGTTTC+AGG - chr3_4:18639949-18639968 None:intergenic 40.0%
ATGTGTAAGCAAGGGTAAGT+TGG - chr3_4:18639060-18639079 None:intergenic 40.0%
AAGCAGCTGAGAAAAACTTC+AGG - chr3_4:18639197-18639216 None:intergenic 40.0%
TCAGCTGCTTGATTAAACCA+TGG + chr3_4:18639207-18639226 Msa0493500:intron 40.0%
!! GTCAGCCAAGAGTATTACAA+TGG + chr3_4:18639282-18639301 Msa0493500:intron 40.0%
GAGTATTACAATGGTTGTCG+GGG + chr3_4:18639291-18639310 Msa0493500:intron 40.0%
ATCTTAGGAAGCTAAGGCTT+TGG - chr3_4:18639376-18639395 None:intergenic 40.0%
TCAGGAATCTTAGGAAGCTA+AGG - chr3_4:18639382-18639401 None:intergenic 40.0%
!! AATTCTGGCTTAGGCAATTC+AGG - chr3_4:18639400-18639419 None:intergenic 40.0%
TTTGGCAATTCAGGTACCTT+AGG - chr3_4:18639424-18639443 None:intergenic 40.0%
GCCAAAGCAAGAATTACCTA+AGG + chr3_4:18639438-18639457 Msa0493500:CDS 40.0%
TTTGGAAGTTCAGGTACCTT+AGG - chr3_4:18639457-18639476 None:intergenic 40.0%
ACTTTAGGTAGCTCAGGTTT+GGG - chr3_4:18639583-18639602 None:intergenic 40.0%
ATAGACAATTCGGGGACTTT+AGG - chr3_4:18639598-18639617 None:intergenic 40.0%
! TTTCTGGCATAGACAATTCG+GGG - chr3_4:18639606-18639625 None:intergenic 40.0%
TTTGGCAATTCAGGGACTTT+AGG - chr3_4:18639703-18639722 None:intergenic 40.0%
! ACCTTAGGGATATCTGGTTT+GGG - chr3_4:18639754-18639773 None:intergenic 40.0%
TACCTTAGGGATATCTGGTT+TGG - chr3_4:18639755-18639774 None:intergenic 40.0%
! TTGGTAACTCAGGTACCTTA+GGG - chr3_4:18639768-18639787 None:intergenic 40.0%
! TTTGGTAACTCAGGTACCTT+AGG - chr3_4:18639769-18639788 None:intergenic 40.0%
TCAGGTACCTTTGGTATTTC+TGG - chr3_4:18639826-18639845 None:intergenic 40.0%
TCAGGGAATTTAGGTAACTC+GGG - chr3_4:18639931-18639950 None:intergenic 40.0%
! GGAACATCTTTAGGAGTTTC+AGG - chr3_4:18639949-18639968 None:intergenic 40.0%
! TCTAGAGTCGAACATACCTG+AGG + chr3_4:18639324-18639343 Msa0493500:intron 45.0%
! AGCTAAGGCTTTGGTATAGG+TGG - chr3_4:18639367-18639386 None:intergenic 45.0%
! GGAAGCTAAGGCTTTGGTAT+AGG - chr3_4:18639370-18639389 None:intergenic 45.0%
!! ACCTTAGGCAATTCTGGCTT+AGG - chr3_4:18639409-18639428 None:intergenic 45.0%
TCAGGTACCTTAGGCAATTC+TGG - chr3_4:18639415-18639434 None:intergenic 45.0%
AACTTAGGTAGCTCAGGCTT+TGG - chr3_4:18639475-18639494 None:intergenic 45.0%
!! AACTCGGGTTTTGGCAATTC+AGG - chr3_4:18639505-18639524 None:intergenic 45.0%
!! ACTTTGGGAAACTCGGGTTT+TGG - chr3_4:18639514-18639533 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+GGG - chr3_4:18639529-18639548 None:intergenic 45.0%
TTTAGGCAACTCGGGAACTT+TGG - chr3_4:18639530-18639549 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+AGG - chr3_4:18639529-18639548 None:intergenic 45.0%
TTCAGGGAACTTAGGCAACT+CGG - chr3_4:18639557-18639576 None:intergenic 45.0%
TTGGGCAATTCAGGGAACTT+AGG - chr3_4:18639565-18639584 None:intergenic 45.0%
GACTTTAGGTAGCTCAGGTT+TGG - chr3_4:18639584-18639603 None:intergenic 45.0%
! AACTCAGGCTTTGGCAATTC+AGG - chr3_4:18639640-18639659 None:intergenic 45.0%
!!! ACTTTAGGTTGCTCGGGTTT+TGG - chr3_4:18639688-18639707 None:intergenic 45.0%
!! TTCAGGGACTTTAGGTTGCT+CGG - chr3_4:18639695-18639714 None:intergenic 45.0%
TTGGGCAACTCAGAGACTTT+AGG - chr3_4:18639736-18639755 None:intergenic 45.0%
TCAGGTACCTTAGGGATATC+TGG - chr3_4:18639760-18639779 None:intergenic 45.0%
!! ACTTTTGGTAGCTCGGGTTT+TGG - chr3_4:18639787-18639806 None:intergenic 45.0%
!! TTTCTGGTTTGGGCAACTCA+GGG - chr3_4:18639810-18639829 None:intergenic 45.0%
!! ATTTCTGGTTTGGGCAACTC+AGG - chr3_4:18639811-18639830 None:intergenic 45.0%
GCCCAAACCAGAAATACCAA+AGG + chr3_4:18639816-18639835 Msa0493500:CDS 45.0%
! TCTAGAGTCGAACATACCTG+AGG + chr3_4:18639324-18639343 Msa0493500:intron 45.0%
! AGCTAAGGCTTTGGTATAGG+TGG - chr3_4:18639367-18639386 None:intergenic 45.0%
! GGAAGCTAAGGCTTTGGTAT+AGG - chr3_4:18639370-18639389 None:intergenic 45.0%
!! ACCTTAGGCAATTCTGGCTT+AGG - chr3_4:18639409-18639428 None:intergenic 45.0%
TCAGGTACCTTAGGCAATTC+TGG - chr3_4:18639415-18639434 None:intergenic 45.0%
AACTTAGGTAGCTCAGGCTT+TGG - chr3_4:18639475-18639494 None:intergenic 45.0%
!! AACTCGGGTTTTGGCAATTC+AGG - chr3_4:18639505-18639524 None:intergenic 45.0%
!! ACTTTGGGAAACTCGGGTTT+TGG - chr3_4:18639514-18639533 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+GGG - chr3_4:18639529-18639548 None:intergenic 45.0%
TTTAGGCAACTCGGGAACTT+TGG - chr3_4:18639530-18639549 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+AGG - chr3_4:18639529-18639548 None:intergenic 45.0%
TTCAGGGAACTTAGGCAACT+CGG - chr3_4:18639557-18639576 None:intergenic 45.0%
TTGGGCAATTCAGGGAACTT+AGG - chr3_4:18639565-18639584 None:intergenic 45.0%
GACTTTAGGTAGCTCAGGTT+TGG - chr3_4:18639584-18639603 None:intergenic 45.0%
! AACTCAGGCTTTGGCAATTC+AGG - chr3_4:18639640-18639659 None:intergenic 45.0%
!!! ACTTTAGGTTGCTCGGGTTT+TGG - chr3_4:18639688-18639707 None:intergenic 45.0%
!! TTCAGGGACTTTAGGTTGCT+CGG - chr3_4:18639695-18639714 None:intergenic 45.0%
TTGGGCAACTCAGAGACTTT+AGG - chr3_4:18639736-18639755 None:intergenic 45.0%
TCAGGTACCTTAGGGATATC+TGG - chr3_4:18639760-18639779 None:intergenic 45.0%
!! ACTTTTGGTAGCTCGGGTTT+TGG - chr3_4:18639787-18639806 None:intergenic 45.0%
!! TTTCTGGTTTGGGCAACTCA+GGG - chr3_4:18639810-18639829 None:intergenic 45.0%
!! ATTTCTGGTTTGGGCAACTC+AGG - chr3_4:18639811-18639830 None:intergenic 45.0%
GCCCAAACCAGAAATACCAA+AGG + chr3_4:18639816-18639835 Msa0493500:CDS 45.0%
GCCTAAGCCAGAATTGCCTA+AGG + chr3_4:18639405-18639424 Msa0493500:intron 50.0%
!! AGCTCAGGCTTTGGAAGTTC+AGG - chr3_4:18639466-18639485 None:intergenic 50.0%
! TCGGGAACTTTGGGAAACTC+GGG - chr3_4:18639520-18639539 None:intergenic 50.0%
! CTCGGGAACTTTGGGAAACT+CGG - chr3_4:18639521-18639540 None:intergenic 50.0%
TCGGGAACTTTAGGCAACTC+GGG - chr3_4:18639538-18639557 None:intergenic 50.0%
CTCGGGAACTTTAGGCAACT+CGG - chr3_4:18639539-18639558 None:intergenic 50.0%
TCAGGGAACTTAGGCAACTC+GGG - chr3_4:18639556-18639575 None:intergenic 50.0%
GCTCAGGTTTGGGCAATTCA+GGG - chr3_4:18639573-18639592 None:intergenic 50.0%
AGCTCAGGTTTGGGCAATTC+AGG - chr3_4:18639574-18639593 None:intergenic 50.0%
!! CTCGGGTTTTGGCAATTCAG+GGG - chr3_4:18639503-18639522 None:intergenic 50.0%
!! GCTCGGGTTTTGGCAATTCA+GGG - chr3_4:18639678-18639697 None:intergenic 50.0%
!! TGCTCGGGTTTTGGCAATTC+AGG - chr3_4:18639679-18639698 None:intergenic 50.0%
!! TCAGGGACTTTAGGTTGCTC+GGG - chr3_4:18639694-18639713 None:intergenic 50.0%
GCCCAAACCAGATATCCCTA+AGG + chr3_4:18639750-18639769 Msa0493500:CDS 50.0%
!! AGCTCGGGTTTTGGTAACTC+AGG - chr3_4:18639778-18639797 None:intergenic 50.0%
!! TCAGGGACTTTTGGTAGCTC+GGG - chr3_4:18639793-18639812 None:intergenic 50.0%
!! CTCAGGGACTTTTGGTAGCT+CGG - chr3_4:18639794-18639813 None:intergenic 50.0%
! TTGGGCAACTCAGGGACTTT+TGG - chr3_4:18639802-18639821 None:intergenic 50.0%
!! ACTCGGGTTTTGGCAGTTCA+GGG - chr3_4:18639915-18639934 None:intergenic 50.0%
!! AACTCGGGTTTTGGCAGTTC+AGG - chr3_4:18639916-18639935 None:intergenic 50.0%
GCCTAAGCCAGAATTGCCTA+AGG + chr3_4:18639405-18639424 Msa0493500:intron 50.0%
!! AGCTCAGGCTTTGGAAGTTC+AGG - chr3_4:18639466-18639485 None:intergenic 50.0%
! TCGGGAACTTTGGGAAACTC+GGG - chr3_4:18639520-18639539 None:intergenic 50.0%
! CTCGGGAACTTTGGGAAACT+CGG - chr3_4:18639521-18639540 None:intergenic 50.0%
TCGGGAACTTTAGGCAACTC+GGG - chr3_4:18639538-18639557 None:intergenic 50.0%
CTCGGGAACTTTAGGCAACT+CGG - chr3_4:18639539-18639558 None:intergenic 50.0%
TCAGGGAACTTAGGCAACTC+GGG - chr3_4:18639556-18639575 None:intergenic 50.0%
GCTCAGGTTTGGGCAATTCA+GGG - chr3_4:18639573-18639592 None:intergenic 50.0%
AGCTCAGGTTTGGGCAATTC+AGG - chr3_4:18639574-18639593 None:intergenic 50.0%
!! CTCGGGTTTTGGCAATTCAG+GGG - chr3_4:18639503-18639522 None:intergenic 50.0%
!! GCTCGGGTTTTGGCAATTCA+GGG - chr3_4:18639678-18639697 None:intergenic 50.0%
!! TGCTCGGGTTTTGGCAATTC+AGG - chr3_4:18639679-18639698 None:intergenic 50.0%
!! TCAGGGACTTTAGGTTGCTC+GGG - chr3_4:18639694-18639713 None:intergenic 50.0%
GCCCAAACCAGATATCCCTA+AGG + chr3_4:18639750-18639769 Msa0493500:CDS 50.0%
!! AGCTCGGGTTTTGGTAACTC+AGG - chr3_4:18639778-18639797 None:intergenic 50.0%
!! TCAGGGACTTTTGGTAGCTC+GGG - chr3_4:18639793-18639812 None:intergenic 50.0%
!! CTCAGGGACTTTTGGTAGCT+CGG - chr3_4:18639794-18639813 None:intergenic 50.0%
! TTGGGCAACTCAGGGACTTT+TGG - chr3_4:18639802-18639821 None:intergenic 50.0%
!! ACTCGGGTTTTGGCAGTTCA+GGG - chr3_4:18639915-18639934 None:intergenic 50.0%
!! AACTCGGGTTTTGGCAGTTC+AGG - chr3_4:18639916-18639935 None:intergenic 50.0%
TCGGGGACTTTAGGTAGCTC+AGG - chr3_4:18639589-18639608 None:intergenic 55.0%
TCGGGGACTTTAGGTAGCTC+AGG - chr3_4:18639589-18639608 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 18638870 18639984 18638870 ID=Msa0493500;Name=Msa0493500
chr3_4 mRNA 18638870 18639984 18638870 ID=Msa0493500-mRNA-1;Parent=Msa0493500;Name=Msa0493500-mRNA-1;_AED=0.34;_eAED=0.35;_QI=0|0|0|0.5|1|1|2|0|212
chr3_4 exon 18638870 18638939 18638870 ID=Msa0493500-mRNA-1:exon:5107;Parent=Msa0493500-mRNA-1
chr3_4 exon 18639416 18639984 18639416 ID=Msa0493500-mRNA-1:exon:5108;Parent=Msa0493500-mRNA-1
chr3_4 CDS 18638870 18638939 18638870 ID=Msa0493500-mRNA-1:cds;Parent=Msa0493500-mRNA-1
chr3_4 CDS 18639416 18639984 18639416 ID=Msa0493500-mRNA-1:cds;Parent=Msa0493500-mRNA-1
Gene Sequence

>Msa0493500

ATGGTTCAATTGGCTTTGAGATTGCTTTCTCTTTTAGAACACAACCTTCCAAATTTCAATGCAGATATAGAATTGCCTAAGGTACCTGAATTGCCAAAGCAAGAATTACCTAAGGTACCTGAACTTCCAAAGCCTGAGCTACCTAAGTTTAATGTTCCTGAATTGCCAAAACCCGAGTTTCCCAAAGTTCCCGAGTTGCCTAAAGTTCCCGAGTTGCCTAAGTTCCCTGAATTGCCCAAACCTGAGCTACCTAAAGTCCCCGAATTGTCTATGCCAGAAATTCCAAAGATACCTGAATTGCCAAAGCCTGAGTTACCTAAATTTAATGCCCCTGAATTGCCAAAACCCGAGCAACCTAAAGTCCCTGAATTGCCAAAACATGAACTACCTAAAGTCTCTGAGTTGCCCAAACCAGATATCCCTAAGGTACCTGAGTTACCAAAACCCGAGCTACCAAAAGTCCCTGAGTTGCCCAAACCAGAAATACCAAAGGTACCTGAATTACCAAAGCTAGAGTTACCTAAGATACCTGAATTGAAAAAGCCTGAACTACCTAAAGTTAATGTCCCTGAACTGCCAAAACCCGAGTTACCTAAATTCCCTGAAACTCCTAAAGATGTTCCAACCAATACTCCTTAA
Protein sequence

>Msa0493500

MVQLALRLLSLLEHNLPNFNADIELPKVPELPKQELPKVPELPKPELPKFNVPELPKPEFPKVPELPKVPELPKFPELPKPELPKVPELSMPEIPKIPELPKPELPKFNAPELPKPEQPKVPELPKHELPKVSELPKPDIPKVPELPKPELPKVPELPKPEIPKVPELPKLELPKIPELKKPELPKVNVPELPKPELPKFPETPKDVPTNTP*