AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0351890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0351890 MtrunA17_Chr3g0087151 85.932 263 20 2 1 257 1 252 1.99e-124 353
Msa0351890 MtrunA17_Chr3g0087231 55.814 344 65 7 1 257 1 344 3.04e-84 255
Msa0351890 MtrunA17_Chr3g0087211 54.444 360 61 9 1 257 1 360 5.47e-81 247
Msa0351890 MtrunA17_Chr3g0087041 79.397 199 19 2 1 199 1 177 3.57e-80 239
Msa0351890 MtrunA17_Chr3g0087041 68.153 157 34 6 108 257 37 184 5.44e-35 124
Msa0351890 MtrunA17_Chr3g0087161 53.271 107 45 2 1 107 1 102 1.95e-25 97.4
Msa0351890 MtrunA17_Chr4g0057451 20.091 219 161 5 28 237 214 427 2.22e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0351890 AT5G09530.1 61.135 229 67 5 26 250 30 240 8.06e-56 183
Msa0351890 AT5G09530.1 56.654 263 64 8 35 250 100 359 5.51e-53 176
Msa0351890 AT5G09530.1 60.440 91 28 2 35 119 282 370 2.94e-15 75.1
Msa0351890 AT5G09520.1 46.809 141 51 3 8 148 10 126 3.02e-23 92.0
Msa0351890 AT5G09520.1 54.545 99 39 2 152 249 37 130 8.17e-16 72.4
Msa0351890 AT5G09520.1 53.684 95 37 2 110 204 41 128 3.19e-15 70.9
Msa0351890 AT5G09520.1 53.922 102 40 2 136 237 34 128 5.18e-15 70.1
Msa0351890 AT5G09520.1 57.955 88 34 2 171 257 34 119 5.22e-14 67.4
Msa0351890 AT5G09520.1 51.546 97 35 3 104 195 41 130 4.97e-12 62.0

Find 95 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTACCTTTGGTATTTC+TGG 0.080700 3_1:-19343511 None:intergenic
TCGGGTATCTTTGGAATTTC+TGG 0.088558 3_1:-19343274 None:intergenic
AGCTCAGGTTTGGGCAATTC+AGG 0.197717 3_1:-19343226 None:intergenic
GGAACATCTTTAGGAGTTTC+AGG 0.202154 3_1:-19343616 None:intergenic
TCTGGTACCTTAGGCAATTC+TGG 0.225262 3_1:-19343067 None:intergenic
AATTCTGGCTTTGGCAATTC+TGG 0.227866 3_1:-19343085 None:intergenic
AATTCAGGGGCATTAAATTT+AGG 0.233034 3_1:-19343316 None:intergenic
TCAGGTACTTTAGGTAATTC+TGG 0.242157 3_1:-19343100 None:intergenic
AACTCTAGCTTTGGTAATTC+AGG 0.247874 3_1:-19343529 None:intergenic
ACCTTTGGTATTTCTGGTTT+GGG 0.248136 3_1:-19343505 None:intergenic
AGTTCAGGCTTTACCAATTC+AGG 0.261051 3_1:-19343562 None:intergenic
GACTTTAGGTAGCTCAGGTT+TGG 0.265600 3_1:-19343236 None:intergenic
TTTGGCAATTCGGGTATCTT+TGG 0.268546 3_1:-19343283 None:intergenic
TACCTTTGGTATTTCTGGTT+TGG 0.269930 3_1:-19343506 None:intergenic
TTTGGAAGTTCAGGTACTTT+AGG 0.279783 3_1:-19343109 None:intergenic
TTTGGCAATTCAGGGACTTT+AGG 0.301883 3_1:-19343355 None:intergenic
TTAGGTAATTCAGGAATCTT+AGG 0.302765 3_1:-19343043 None:intergenic
TACCTTAGGGATATCTGGTT+TGG 0.306919 3_1:-19343407 None:intergenic
ATAGACAATTCGGGGACTTT+AGG 0.311738 3_1:-19343250 None:intergenic
ACTTTAGGTGGCTCGAGTTT+TGG 0.314784 3_1:-19343340 None:intergenic
GTATTGGTTGGAACATCTTT+AGG 0.328990 3_1:-19343625 None:intergenic
TTAGGCAACTCGGGAACTTT+AGG 0.329308 3_1:-19343199 None:intergenic
TTCGGTAACTCGGGTACCTT+AGG 0.331068 3_1:-19343421 None:intergenic
TTCAGGTACCTTAGGTAGTT+CGG 0.333628 3_1:-19343446 None:intergenic
TCGGGTACCTTAGGGATATC+TGG 0.340073 3_1:-19343412 None:intergenic
AGCTCGGGCTTTGGAAGTTC+AGG 0.341805 3_1:-19343118 None:intergenic
AGTATAGCTAGAATCATTAA+TGG 0.349622 3_1:-19342914 None:intergenic
TCAGGTACCTTAGGTAGTTC+GGG 0.349878 3_1:-19343445 None:intergenic
AAGTCGGACTTTGGCAATTC+GGG 0.351787 3_1:-19343292 None:intergenic
ATAGGTGGAAAATCAAGTTT+TGG 0.353325 3_1:-19343004 None:intergenic
ACTTTAGGTAGTTCATGTTT+TGG 0.353892 3_1:-19343373 None:intergenic
ACCTTAGGTAGTTCGGGTTT+CGG 0.357170 3_1:-19343439 None:intergenic
ACTTTGGGAAACTCGGGTTT+TGG 0.357337 3_1:-19343166 None:intergenic
TTAGGCAACTCAGGAACTTT+GGG 0.358876 3_1:-19343181 None:intergenic
TTGGGCAACTCAGAGACTTT+AGG 0.363342 3_1:-19343388 None:intergenic
ACTTTAGGTAGCTCAGGTTT+GGG 0.365399 3_1:-19343235 None:intergenic
AATTTCTGGCATAGACAATT+CGG 0.365865 3_1:-19343260 None:intergenic
ACATTAACTTTAGGTAGTTC+AGG 0.370017 3_1:-19343577 None:intergenic
GGAAGCTCAGGCTTTGGTAT+AGG 0.376080 3_1:-19343022 None:intergenic
AATTCTGGCTTAGGTAATTC+AGG 0.380278 3_1:-19343052 None:intergenic
TAAGTCGGACTTTGGCAATT+CGG 0.380730 3_1:-19343293 None:intergenic
TTGGGCAACTCAGAGACTTT+TGG 0.383888 3_1:-19343487 None:intergenic
CTCAGGAACTTTGGGAAACT+CGG 0.389158 3_1:-19343173 None:intergenic
ACTTTAGGTAATTCTGGCTT+TGG 0.389572 3_1:-19343094 None:intergenic
TTTAGGCAACTCAGGAACTT+TGG 0.393082 3_1:-19343182 None:intergenic
ATCTTAGGAAGCTCAGGCTT+TGG 0.394512 3_1:-19343028 None:intergenic
GGGTCAGGGACATTAACTTT+AGG 0.397411 3_1:-19343586 None:intergenic
ATTTCTGGCATAGACAATTC+GGG 0.397522 3_1:-19343259 None:intergenic
TTTGGTAATTCAGGTACCTT+TGG 0.399783 3_1:-19343520 None:intergenic
AACTTAGGTAGCTCGGGCTT+TGG 0.403695 3_1:-19343127 None:intergenic
TTTGGCAATTCTGGTACCTT+AGG 0.412348 3_1:-19343076 None:intergenic
ACCTTAGGGATATCTGGTTT+GGG 0.420537 3_1:-19343406 None:intergenic
TTTACCAATTCAGGTACCTT+AGG 0.428581 3_1:-19343553 None:intergenic
GTTACCTAAGGTACCTGAAT+TGG 0.434651 3_1:+19343549 Msa0351890:CDS
ACCTTAGGTAACTCTAGCTT+TGG 0.436884 3_1:-19343538 None:intergenic
AGTTCCAACTTAAGGAGTAT+TGG 0.438613 3_1:-19343641 None:intergenic
AGAGTATTACAATGGTTGTC+GGG 0.440611 3_1:+19342945 Msa0351890:CDS
TTTGGTAATTCAGGTACCTT+AGG 0.451961 3_1:-19343454 None:intergenic
TAGTTCGGGTTTCGGTAACT+CGG 0.452100 3_1:-19343431 None:intergenic
TCAGGAATCTTAGGAAGCTC+AGG 0.453881 3_1:-19343034 None:intergenic
GTTTCAGGGTAACTTGGGTC+AGG 0.457358 3_1:-19343601 None:intergenic
TTAGGAGTTTCAGGGTAACT+TGG 0.458417 3_1:-19343607 None:intergenic
AATTTAGGTAAGTCGGACTT+TGG 0.460942 3_1:-19343301 None:intergenic
AACATTAAACTTAGGTAGCT+CGG 0.462347 3_1:-19343134 None:intergenic
ACCTTAGGCAATTCTGGCTT+AGG 0.463421 3_1:-19343061 None:intergenic
TCGGGGACTTTAGGTAGCTC+AGG 0.467048 3_1:-19343241 None:intergenic
TTGGGCAATTCAGGGAACTT+AGG 0.475381 3_1:-19343217 None:intergenic
GGCAATTCAGGGACTTTAGG+TGG 0.484066 3_1:-19343352 None:intergenic
GGCATTAAATTTAGGTAAGT+CGG 0.487607 3_1:-19343308 None:intergenic
GACAACCATTGTAATACTCT+TGG 0.489157 3_1:-19342942 None:intergenic
AATTCAGGAACATTAAACTT+AGG 0.490699 3_1:-19343142 None:intergenic
ACATTAAACTTAGGTAGCTC+GGG 0.491103 3_1:-19343133 None:intergenic
TTCAGGGAACTTAGGCAACT+CGG 0.499436 3_1:-19343209 None:intergenic
GAACATCTTTAGGAGTTTCA+GGG 0.499674 3_1:-19343615 None:intergenic
AACCATTGTAATACTCTTGG+CGG 0.510875 3_1:-19342939 None:intergenic
TCGGTAACTCGGGTACCTTA+GGG 0.515191 3_1:-19343420 None:intergenic
GCTCAGGTTTGGGCAATTCA+GGG 0.523757 3_1:-19343225 None:intergenic
CCAACTTAAGGAGTATTGGT+TGG 0.525013 3_1:-19343637 None:intergenic
AAGAGTATTACAATGGTTGT+CGG 0.526376 3_1:+19342944 Msa0351890:CDS
TCAGGAACTTTGGGAAACTC+GGG 0.528389 3_1:-19343172 None:intergenic
TAGGAGTTTCAGGGTAACTT+GGG 0.530415 3_1:-19343606 None:intergenic
AGCTCAGGCTTTGGTATAGG+TGG 0.530445 3_1:-19343019 None:intergenic
ACCGAAACCCGAACTACCTA+AGG 0.555888 3_1:+19343438 Msa0351890:CDS
TCGGGAACTTTAGGCAACTC+AGG 0.569614 3_1:-19343190 None:intergenic
TCAGGGAACTTAGGCAACTC+GGG 0.582113 3_1:-19343208 None:intergenic
ACCTAAGCCAGAATTGCCTA+AGG 0.603337 3_1:+19343060 Msa0351890:CDS
AGTTCGGGTTTCGGTAACTC+GGG 0.606735 3_1:-19343430 None:intergenic
TTTCTGGCATAGACAATTCG+GGG 0.611978 3_1:-19343258 None:intergenic
ACCAAAGCTAGAGTTACCTA+AGG 0.616598 3_1:+19343537 Msa0351890:CDS
TTTCAGGGTAACTTGGGTCA+GGG 0.623182 3_1:-19343600 None:intergenic
GAGTATTACAATGGTTGTCG+GGG 0.633099 3_1:+19342946 Msa0351890:CDS
GCCCAAACCAGATATCCCTA+AGG 0.649847 3_1:+19343405 Msa0351890:CDS
ATCCGCCAAGAGTATTACAA+TGG 0.672419 3_1:+19342937 Msa0351890:CDS
GCCCAAACCAGAAATACCAA+AGG 0.685777 3_1:+19343504 Msa0351890:CDS
TCTAGAGTCGAACATACCTG+AGG 0.701502 3_1:+19342979 Msa0351890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATGGAAAAATAATTTT+TGG - chr3_1:19342902-19342921 None:intergenic 10.0%
!!! ATTAATGGAAAAATAATTTT+TGG - chr3_1:19342902-19342921 None:intergenic 10.0%
! AGTATAGCTAGAATCATTAA+TGG - chr3_1:19342917-19342936 None:intergenic 25.0%
! AATTCAGGAACATTAAACTT+AGG - chr3_1:19343145-19343164 None:intergenic 25.0%
! AGTATAGCTAGAATCATTAA+TGG - chr3_1:19342917-19342936 None:intergenic 25.0%
! AATTCAGGAACATTAAACTT+AGG - chr3_1:19343145-19343164 None:intergenic 25.0%
!! AAGAGTATTACAATGGTTGT+CGG + chr3_1:19342944-19342963 Msa0351890:CDS 30.0%
! ATAGGTGGAAAATCAAGTTT+TGG - chr3_1:19343007-19343026 None:intergenic 30.0%
TTAGGTAATTCAGGAATCTT+AGG - chr3_1:19343046-19343065 None:intergenic 30.0%
AACATTAAACTTAGGTAGCT+CGG - chr3_1:19343137-19343156 None:intergenic 30.0%
!! AATTTCTGGCATAGACAATT+CGG - chr3_1:19343263-19343282 None:intergenic 30.0%
GGCATTAAATTTAGGTAAGT+CGG - chr3_1:19343311-19343330 None:intergenic 30.0%
!! AATTCAGGGGCATTAAATTT+AGG - chr3_1:19343319-19343338 None:intergenic 30.0%
! ACTTTAGGTAGTTCATGTTT+TGG - chr3_1:19343376-19343395 None:intergenic 30.0%
ACATTAACTTTAGGTAGTTC+AGG - chr3_1:19343580-19343599 None:intergenic 30.0%
!! AAGAGTATTACAATGGTTGT+CGG + chr3_1:19342944-19342963 Msa0351890:CDS 30.0%
! ATAGGTGGAAAATCAAGTTT+TGG - chr3_1:19343007-19343026 None:intergenic 30.0%
TTAGGTAATTCAGGAATCTT+AGG - chr3_1:19343046-19343065 None:intergenic 30.0%
AACATTAAACTTAGGTAGCT+CGG - chr3_1:19343137-19343156 None:intergenic 30.0%
!! AATTTCTGGCATAGACAATT+CGG - chr3_1:19343263-19343282 None:intergenic 30.0%
GGCATTAAATTTAGGTAAGT+CGG - chr3_1:19343311-19343330 None:intergenic 30.0%
!! AATTCAGGGGCATTAAATTT+AGG - chr3_1:19343319-19343338 None:intergenic 30.0%
! ACTTTAGGTAGTTCATGTTT+TGG - chr3_1:19343376-19343395 None:intergenic 30.0%
ACATTAACTTTAGGTAGTTC+AGG - chr3_1:19343580-19343599 None:intergenic 30.0%
AACCATTGTAATACTCTTGG+CGG - chr3_1:19342942-19342961 None:intergenic 35.0%
GACAACCATTGTAATACTCT+TGG - chr3_1:19342945-19342964 None:intergenic 35.0%
! AGAGTATTACAATGGTTGTC+GGG + chr3_1:19342945-19342964 Msa0351890:CDS 35.0%
!!! AAATCAAGTTTTGGAACCTC+AGG - chr3_1:19342998-19343017 None:intergenic 35.0%
!! AATTCTGGCTTAGGTAATTC+AGG - chr3_1:19343055-19343074 None:intergenic 35.0%
!! ACTTTAGGTAATTCTGGCTT+TGG - chr3_1:19343097-19343116 None:intergenic 35.0%
TCAGGTACTTTAGGTAATTC+TGG - chr3_1:19343103-19343122 None:intergenic 35.0%
TTTGGAAGTTCAGGTACTTT+AGG - chr3_1:19343112-19343131 None:intergenic 35.0%
ACATTAAACTTAGGTAGCTC+GGG - chr3_1:19343136-19343155 None:intergenic 35.0%
!! ATTTCTGGCATAGACAATTC+GGG - chr3_1:19343262-19343281 None:intergenic 35.0%
AATTTAGGTAAGTCGGACTT+TGG - chr3_1:19343304-19343323 None:intergenic 35.0%
!!! GTTCATGTTTTGGCAATTCA+GGG - chr3_1:19343366-19343385 None:intergenic 35.0%
!!! AGTTCATGTTTTGGCAATTC+AGG - chr3_1:19343367-19343386 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+AGG - chr3_1:19343457-19343476 None:intergenic 35.0%
!! ACCTTTGGTATTTCTGGTTT+GGG - chr3_1:19343508-19343527 None:intergenic 35.0%
!! TACCTTTGGTATTTCTGGTT+TGG - chr3_1:19343509-19343528 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+TGG - chr3_1:19343457-19343476 None:intergenic 35.0%
!! AACTCTAGCTTTGGTAATTC+AGG - chr3_1:19343532-19343551 None:intergenic 35.0%
TTTACCAATTCAGGTACCTT+AGG - chr3_1:19343556-19343575 None:intergenic 35.0%
! GAACATCTTTAGGAGTTTCA+GGG - chr3_1:19343618-19343637 None:intergenic 35.0%
!! GTATTGGTTGGAACATCTTT+AGG - chr3_1:19343628-19343647 None:intergenic 35.0%
AACCATTGTAATACTCTTGG+CGG - chr3_1:19342942-19342961 None:intergenic 35.0%
GACAACCATTGTAATACTCT+TGG - chr3_1:19342945-19342964 None:intergenic 35.0%
! AGAGTATTACAATGGTTGTC+GGG + chr3_1:19342945-19342964 Msa0351890:CDS 35.0%
!!! AAATCAAGTTTTGGAACCTC+AGG - chr3_1:19342998-19343017 None:intergenic 35.0%
!! AATTCTGGCTTAGGTAATTC+AGG - chr3_1:19343055-19343074 None:intergenic 35.0%
!! ACTTTAGGTAATTCTGGCTT+TGG - chr3_1:19343097-19343116 None:intergenic 35.0%
TCAGGTACTTTAGGTAATTC+TGG - chr3_1:19343103-19343122 None:intergenic 35.0%
TTTGGAAGTTCAGGTACTTT+AGG - chr3_1:19343112-19343131 None:intergenic 35.0%
ACATTAAACTTAGGTAGCTC+GGG - chr3_1:19343136-19343155 None:intergenic 35.0%
!! ATTTCTGGCATAGACAATTC+GGG - chr3_1:19343262-19343281 None:intergenic 35.0%
AATTTAGGTAAGTCGGACTT+TGG - chr3_1:19343304-19343323 None:intergenic 35.0%
!!! GTTCATGTTTTGGCAATTCA+GGG - chr3_1:19343366-19343385 None:intergenic 35.0%
!!! AGTTCATGTTTTGGCAATTC+AGG - chr3_1:19343367-19343386 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+AGG - chr3_1:19343457-19343476 None:intergenic 35.0%
!! ACCTTTGGTATTTCTGGTTT+GGG - chr3_1:19343508-19343527 None:intergenic 35.0%
!! TACCTTTGGTATTTCTGGTT+TGG - chr3_1:19343509-19343528 None:intergenic 35.0%
! TTTGGTAATTCAGGTACCTT+TGG - chr3_1:19343457-19343476 None:intergenic 35.0%
!! AACTCTAGCTTTGGTAATTC+AGG - chr3_1:19343532-19343551 None:intergenic 35.0%
TTTACCAATTCAGGTACCTT+AGG - chr3_1:19343556-19343575 None:intergenic 35.0%
! GAACATCTTTAGGAGTTTCA+GGG - chr3_1:19343618-19343637 None:intergenic 35.0%
!! GTATTGGTTGGAACATCTTT+AGG - chr3_1:19343628-19343647 None:intergenic 35.0%
!! ATCCGCCAAGAGTATTACAA+TGG + chr3_1:19342937-19342956 Msa0351890:CDS 40.0%
GAGTATTACAATGGTTGTCG+GGG + chr3_1:19342946-19342965 Msa0351890:CDS 40.0%
TTTGGCAATTCTGGTACCTT+AGG - chr3_1:19343079-19343098 None:intergenic 40.0%
!! AATTCTGGCTTTGGCAATTC+TGG - chr3_1:19343088-19343107 None:intergenic 40.0%
TTAGGCAACTCAGGAACTTT+GGG - chr3_1:19343184-19343203 None:intergenic 40.0%
TTTAGGCAACTCAGGAACTT+TGG - chr3_1:19343185-19343204 None:intergenic 40.0%
ACTTTAGGTAGCTCAGGTTT+GGG - chr3_1:19343238-19343257 None:intergenic 40.0%
ATAGACAATTCGGGGACTTT+AGG - chr3_1:19343253-19343272 None:intergenic 40.0%
! TTTCTGGCATAGACAATTCG+GGG - chr3_1:19343261-19343280 None:intergenic 40.0%
!! TCGGGTATCTTTGGAATTTC+TGG - chr3_1:19343277-19343296 None:intergenic 40.0%
TTTGGCAATTCGGGTATCTT+TGG - chr3_1:19343286-19343305 None:intergenic 40.0%
TAAGTCGGACTTTGGCAATT+CGG - chr3_1:19343296-19343315 None:intergenic 40.0%
TTTGGCAATTCAGGGACTTT+AGG - chr3_1:19343358-19343377 None:intergenic 40.0%
! ACCTTAGGGATATCTGGTTT+GGG - chr3_1:19343409-19343428 None:intergenic 40.0%
TACCTTAGGGATATCTGGTT+TGG - chr3_1:19343410-19343429 None:intergenic 40.0%
TTCAGGTACCTTAGGTAGTT+CGG - chr3_1:19343449-19343468 None:intergenic 40.0%
!! AGCTCAGGTTTTGGTAATTC+AGG - chr3_1:19343466-19343485 None:intergenic 40.0%
!! ACTTTTGGTAGCTCAGGTTT+TGG - chr3_1:19343475-19343494 None:intergenic 40.0%
TCAGGTACCTTTGGTATTTC+TGG - chr3_1:19343514-19343533 None:intergenic 40.0%
ACCAAAGCTAGAGTTACCTA+AGG + chr3_1:19343537-19343556 Msa0351890:CDS 40.0%
! ACCTTAGGTAACTCTAGCTT+TGG - chr3_1:19343541-19343560 None:intergenic 40.0%
! GTTACCTAAGGTACCTGAAT+TGG + chr3_1:19343549-19343568 Msa0351890:CDS 40.0%
AGTTCAGGCTTTACCAATTC+AGG - chr3_1:19343565-19343584 None:intergenic 40.0%
TAGGAGTTTCAGGGTAACTT+GGG - chr3_1:19343609-19343628 None:intergenic 40.0%
TTAGGAGTTTCAGGGTAACT+TGG - chr3_1:19343610-19343629 None:intergenic 40.0%
! GGAACATCTTTAGGAGTTTC+AGG - chr3_1:19343619-19343638 None:intergenic 40.0%
!! ATCCGCCAAGAGTATTACAA+TGG + chr3_1:19342937-19342956 Msa0351890:CDS 40.0%
GAGTATTACAATGGTTGTCG+GGG + chr3_1:19342946-19342965 Msa0351890:CDS 40.0%
TTTGGCAATTCTGGTACCTT+AGG - chr3_1:19343079-19343098 None:intergenic 40.0%
!! AATTCTGGCTTTGGCAATTC+TGG - chr3_1:19343088-19343107 None:intergenic 40.0%
TTAGGCAACTCAGGAACTTT+GGG - chr3_1:19343184-19343203 None:intergenic 40.0%
TTTAGGCAACTCAGGAACTT+TGG - chr3_1:19343185-19343204 None:intergenic 40.0%
ACTTTAGGTAGCTCAGGTTT+GGG - chr3_1:19343238-19343257 None:intergenic 40.0%
ATAGACAATTCGGGGACTTT+AGG - chr3_1:19343253-19343272 None:intergenic 40.0%
! TTTCTGGCATAGACAATTCG+GGG - chr3_1:19343261-19343280 None:intergenic 40.0%
!! TCGGGTATCTTTGGAATTTC+TGG - chr3_1:19343277-19343296 None:intergenic 40.0%
TTTGGCAATTCGGGTATCTT+TGG - chr3_1:19343286-19343305 None:intergenic 40.0%
TAAGTCGGACTTTGGCAATT+CGG - chr3_1:19343296-19343315 None:intergenic 40.0%
TTTGGCAATTCAGGGACTTT+AGG - chr3_1:19343358-19343377 None:intergenic 40.0%
! ACCTTAGGGATATCTGGTTT+GGG - chr3_1:19343409-19343428 None:intergenic 40.0%
TACCTTAGGGATATCTGGTT+TGG - chr3_1:19343410-19343429 None:intergenic 40.0%
TTCAGGTACCTTAGGTAGTT+CGG - chr3_1:19343449-19343468 None:intergenic 40.0%
!! AGCTCAGGTTTTGGTAATTC+AGG - chr3_1:19343466-19343485 None:intergenic 40.0%
!! ACTTTTGGTAGCTCAGGTTT+TGG - chr3_1:19343475-19343494 None:intergenic 40.0%
TCAGGTACCTTTGGTATTTC+TGG - chr3_1:19343514-19343533 None:intergenic 40.0%
ACCAAAGCTAGAGTTACCTA+AGG + chr3_1:19343537-19343556 Msa0351890:CDS 40.0%
! ACCTTAGGTAACTCTAGCTT+TGG - chr3_1:19343541-19343560 None:intergenic 40.0%
! GTTACCTAAGGTACCTGAAT+TGG + chr3_1:19343549-19343568 Msa0351890:CDS 40.0%
AGTTCAGGCTTTACCAATTC+AGG - chr3_1:19343565-19343584 None:intergenic 40.0%
TAGGAGTTTCAGGGTAACTT+GGG - chr3_1:19343609-19343628 None:intergenic 40.0%
TTAGGAGTTTCAGGGTAACT+TGG - chr3_1:19343610-19343629 None:intergenic 40.0%
! GGAACATCTTTAGGAGTTTC+AGG - chr3_1:19343619-19343638 None:intergenic 40.0%
! TCTAGAGTCGAACATACCTG+AGG + chr3_1:19342979-19342998 Msa0351890:CDS 45.0%
ATCTTAGGAAGCTCAGGCTT+TGG - chr3_1:19343031-19343050 None:intergenic 45.0%
TCAGGAATCTTAGGAAGCTC+AGG - chr3_1:19343037-19343056 None:intergenic 45.0%
ACCTAAGCCAGAATTGCCTA+AGG + chr3_1:19343060-19343079 Msa0351890:CDS 45.0%
!! ACCTTAGGCAATTCTGGCTT+AGG - chr3_1:19343064-19343083 None:intergenic 45.0%
TCTGGTACCTTAGGCAATTC+TGG - chr3_1:19343070-19343089 None:intergenic 45.0%
!! AACTCGGGTTTTGGCAATTC+AGG - chr3_1:19343160-19343179 None:intergenic 45.0%
!! ACTTTGGGAAACTCGGGTTT+TGG - chr3_1:19343169-19343188 None:intergenic 45.0%
! TCAGGAACTTTGGGAAACTC+GGG - chr3_1:19343175-19343194 None:intergenic 45.0%
! CTCAGGAACTTTGGGAAACT+CGG - chr3_1:19343176-19343195 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+AGG - chr3_1:19343202-19343221 None:intergenic 45.0%
TTCAGGGAACTTAGGCAACT+CGG - chr3_1:19343212-19343231 None:intergenic 45.0%
TTGGGCAATTCAGGGAACTT+AGG - chr3_1:19343220-19343239 None:intergenic 45.0%
GACTTTAGGTAGCTCAGGTT+TGG - chr3_1:19343239-19343258 None:intergenic 45.0%
AAGTCGGACTTTGGCAATTC+GGG - chr3_1:19343295-19343314 None:intergenic 45.0%
!! CTCGAGTTTTGGCAATTCAG+GGG - chr3_1:19343332-19343351 None:intergenic 45.0%
!! GCTCGAGTTTTGGCAATTCA+GGG - chr3_1:19343333-19343352 None:intergenic 45.0%
! ACTTTAGGTGGCTCGAGTTT+TGG - chr3_1:19343343-19343362 None:intergenic 45.0%
TTGGGCAACTCAGAGACTTT+AGG - chr3_1:19343391-19343410 None:intergenic 45.0%
! TAGTTCGGGTTTCGGTAACT+CGG - chr3_1:19343434-19343453 None:intergenic 45.0%
! ACCTTAGGTAGTTCGGGTTT+CGG - chr3_1:19343442-19343461 None:intergenic 45.0%
TCAGGTACCTTAGGTAGTTC+GGG - chr3_1:19343448-19343467 None:intergenic 45.0%
!! TCAGAGACTTTTGGTAGCTC+AGG - chr3_1:19343481-19343500 None:intergenic 45.0%
! TTGGGCAACTCAGAGACTTT+TGG - chr3_1:19343391-19343410 None:intergenic 45.0%
GCCCAAACCAGAAATACCAA+AGG + chr3_1:19343504-19343523 Msa0351890:CDS 45.0%
GGGTCAGGGACATTAACTTT+AGG - chr3_1:19343589-19343608 None:intergenic 45.0%
TTTCAGGGTAACTTGGGTCA+GGG - chr3_1:19343603-19343622 None:intergenic 45.0%
! TCTAGAGTCGAACATACCTG+AGG + chr3_1:19342979-19342998 Msa0351890:CDS 45.0%
ATCTTAGGAAGCTCAGGCTT+TGG - chr3_1:19343031-19343050 None:intergenic 45.0%
TCAGGAATCTTAGGAAGCTC+AGG - chr3_1:19343037-19343056 None:intergenic 45.0%
ACCTAAGCCAGAATTGCCTA+AGG + chr3_1:19343060-19343079 Msa0351890:CDS 45.0%
!! ACCTTAGGCAATTCTGGCTT+AGG - chr3_1:19343064-19343083 None:intergenic 45.0%
TCTGGTACCTTAGGCAATTC+TGG - chr3_1:19343070-19343089 None:intergenic 45.0%
!! AACTCGGGTTTTGGCAATTC+AGG - chr3_1:19343160-19343179 None:intergenic 45.0%
!! ACTTTGGGAAACTCGGGTTT+TGG - chr3_1:19343169-19343188 None:intergenic 45.0%
! TCAGGAACTTTGGGAAACTC+GGG - chr3_1:19343175-19343194 None:intergenic 45.0%
! CTCAGGAACTTTGGGAAACT+CGG - chr3_1:19343176-19343195 None:intergenic 45.0%
TTAGGCAACTCGGGAACTTT+AGG - chr3_1:19343202-19343221 None:intergenic 45.0%
TTCAGGGAACTTAGGCAACT+CGG - chr3_1:19343212-19343231 None:intergenic 45.0%
TTGGGCAATTCAGGGAACTT+AGG - chr3_1:19343220-19343239 None:intergenic 45.0%
GACTTTAGGTAGCTCAGGTT+TGG - chr3_1:19343239-19343258 None:intergenic 45.0%
AAGTCGGACTTTGGCAATTC+GGG - chr3_1:19343295-19343314 None:intergenic 45.0%
!! CTCGAGTTTTGGCAATTCAG+GGG - chr3_1:19343332-19343351 None:intergenic 45.0%
!! GCTCGAGTTTTGGCAATTCA+GGG - chr3_1:19343333-19343352 None:intergenic 45.0%
! ACTTTAGGTGGCTCGAGTTT+TGG - chr3_1:19343343-19343362 None:intergenic 45.0%
TTGGGCAACTCAGAGACTTT+AGG - chr3_1:19343391-19343410 None:intergenic 45.0%
! TAGTTCGGGTTTCGGTAACT+CGG - chr3_1:19343434-19343453 None:intergenic 45.0%
! ACCTTAGGTAGTTCGGGTTT+CGG - chr3_1:19343442-19343461 None:intergenic 45.0%
TCAGGTACCTTAGGTAGTTC+GGG - chr3_1:19343448-19343467 None:intergenic 45.0%
!! TCAGAGACTTTTGGTAGCTC+AGG - chr3_1:19343481-19343500 None:intergenic 45.0%
! TTGGGCAACTCAGAGACTTT+TGG - chr3_1:19343391-19343410 None:intergenic 45.0%
GCCCAAACCAGAAATACCAA+AGG + chr3_1:19343504-19343523 Msa0351890:CDS 45.0%
GGGTCAGGGACATTAACTTT+AGG - chr3_1:19343589-19343608 None:intergenic 45.0%
TTTCAGGGTAACTTGGGTCA+GGG - chr3_1:19343603-19343622 None:intergenic 45.0%
! AGCTCAGGCTTTGGTATAGG+TGG - chr3_1:19343022-19343041 None:intergenic 50.0%
! GGAAGCTCAGGCTTTGGTAT+AGG - chr3_1:19343025-19343044 None:intergenic 50.0%
AACTTAGGTAGCTCGGGCTT+TGG - chr3_1:19343130-19343149 None:intergenic 50.0%
TCGGGAACTTTAGGCAACTC+AGG - chr3_1:19343193-19343212 None:intergenic 50.0%
TCAGGGAACTTAGGCAACTC+GGG - chr3_1:19343211-19343230 None:intergenic 50.0%
GCTCAGGTTTGGGCAATTCA+GGG - chr3_1:19343228-19343247 None:intergenic 50.0%
AGCTCAGGTTTGGGCAATTC+AGG - chr3_1:19343229-19343248 None:intergenic 50.0%
!! GGCTCGAGTTTTGGCAATTC+AGG - chr3_1:19343334-19343353 None:intergenic 50.0%
GGCAATTCAGGGACTTTAGG+TGG - chr3_1:19343355-19343374 None:intergenic 50.0%
GCCCAAACCAGATATCCCTA+AGG + chr3_1:19343405-19343424 Msa0351890:CDS 50.0%
TCGGGTACCTTAGGGATATC+TGG - chr3_1:19343415-19343434 None:intergenic 50.0%
TCGGTAACTCGGGTACCTTA+GGG - chr3_1:19343423-19343442 None:intergenic 50.0%
TTCGGTAACTCGGGTACCTT+AGG - chr3_1:19343424-19343443 None:intergenic 50.0%
! AGTTCGGGTTTCGGTAACTC+GGG - chr3_1:19343433-19343452 None:intergenic 50.0%
ACCGAAACCCGAACTACCTA+AGG + chr3_1:19343438-19343457 Msa0351890:CDS 50.0%
GTTTCAGGGTAACTTGGGTC+AGG - chr3_1:19343604-19343623 None:intergenic 50.0%
! AGCTCAGGCTTTGGTATAGG+TGG - chr3_1:19343022-19343041 None:intergenic 50.0%
! GGAAGCTCAGGCTTTGGTAT+AGG - chr3_1:19343025-19343044 None:intergenic 50.0%
AACTTAGGTAGCTCGGGCTT+TGG - chr3_1:19343130-19343149 None:intergenic 50.0%
TCGGGAACTTTAGGCAACTC+AGG - chr3_1:19343193-19343212 None:intergenic 50.0%
TCAGGGAACTTAGGCAACTC+GGG - chr3_1:19343211-19343230 None:intergenic 50.0%
GCTCAGGTTTGGGCAATTCA+GGG - chr3_1:19343228-19343247 None:intergenic 50.0%
AGCTCAGGTTTGGGCAATTC+AGG - chr3_1:19343229-19343248 None:intergenic 50.0%
!! GGCTCGAGTTTTGGCAATTC+AGG - chr3_1:19343334-19343353 None:intergenic 50.0%
GGCAATTCAGGGACTTTAGG+TGG - chr3_1:19343355-19343374 None:intergenic 50.0%
GCCCAAACCAGATATCCCTA+AGG + chr3_1:19343405-19343424 Msa0351890:CDS 50.0%
TCGGGTACCTTAGGGATATC+TGG - chr3_1:19343415-19343434 None:intergenic 50.0%
TCGGTAACTCGGGTACCTTA+GGG - chr3_1:19343423-19343442 None:intergenic 50.0%
TTCGGTAACTCGGGTACCTT+AGG - chr3_1:19343424-19343443 None:intergenic 50.0%
! AGTTCGGGTTTCGGTAACTC+GGG - chr3_1:19343433-19343452 None:intergenic 50.0%
ACCGAAACCCGAACTACCTA+AGG + chr3_1:19343438-19343457 Msa0351890:CDS 50.0%
GTTTCAGGGTAACTTGGGTC+AGG - chr3_1:19343604-19343623 None:intergenic 50.0%
!! AGCTCGGGCTTTGGAAGTTC+AGG - chr3_1:19343121-19343140 None:intergenic 55.0%
TCGGGGACTTTAGGTAGCTC+AGG - chr3_1:19343244-19343263 None:intergenic 55.0%
!! AGCTCGGGCTTTGGAAGTTC+AGG - chr3_1:19343121-19343140 None:intergenic 55.0%
TCGGGGACTTTAGGTAGCTC+AGG - chr3_1:19343244-19343263 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 19342881 19343654 19342881 ID=Msa0351890;Name=Msa0351890
chr3_1 mRNA 19342881 19343654 19342881 ID=Msa0351890-mRNA-1;Parent=Msa0351890;Name=Msa0351890-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|257
chr3_1 exon 19342881 19343654 19342881 ID=Msa0351890-mRNA-1:exon:4540;Parent=Msa0351890-mRNA-1
chr3_1 CDS 19342881 19343654 19342881 ID=Msa0351890-mRNA-1:cds;Parent=Msa0351890-mRNA-1
Gene Sequence

>Msa0351890

ATGGCTTACATCAATTTGCCAAAAATTATTTTTCCATTAATGATTCTAGCTATACTATCCGCCAAGAGTATTACAATGGTTGTCGGGGCACGCAATCTTCTAGAGTCGAACATACCTGAGGTTCCAAAACTTGATTTTCCACCTATACCAAAGCCTGAGCTTCCTAAGATTCCTGAATTACCTAAGCCAGAATTGCCTAAGGTACCAGAATTGCCAAAGCCAGAATTACCTAAAGTACCTGAACTTCCAAAGCCCGAGCTACCTAAGTTTAATGTTCCTGAATTGCCAAAACCCGAGTTTCCCAAAGTTCCTGAGTTGCCTAAAGTTCCCGAGTTGCCTAAGTTCCCTGAATTGCCCAAACCTGAGCTACCTAAAGTCCCCGAATTGTCTATGCCAGAAATTCCAAAGATACCCGAATTGCCAAAGTCCGACTTACCTAAATTTAATGCCCCTGAATTGCCAAAACTCGAGCCACCTAAAGTCCCTGAATTGCCAAAACATGAACTACCTAAAGTCTCTGAGTTGCCCAAACCAGATATCCCTAAGGTACCCGAGTTACCGAAACCCGAACTACCTAAGGTACCTGAATTACCAAAACCTGAGCTACCAAAAGTCTCTGAGTTGCCCAAACCAGAAATACCAAAGGTACCTGAATTACCAAAGCTAGAGTTACCTAAGGTACCTGAATTGGTAAAGCCTGAACTACCTAAAGTTAATGTCCCTGACCCAAGTTACCCTGAAACTCCTAAAGATGTTCCAACCAATACTCCTTAA
Protein sequence

>Msa0351890

MAYINLPKIIFPLMILAILSAKSITMVVGARNLLESNIPEVPKLDFPPIPKPELPKIPELPKPELPKVPELPKPELPKVPELPKPELPKFNVPELPKPEFPKVPELPKVPELPKFPELPKPELPKVPELSMPEIPKIPELPKSDLPKFNAPELPKLEPPKVPELPKHELPKVSELPKPDIPKVPELPKPELPKVPELPKPELPKVSELPKPEIPKVPELPKLELPKVPELVKPELPKVNVPDPSYPETPKDVPTNTP*