Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0500680 | AES73283.1 | 71.429 | 126 | 9 | 1 | 1 | 126 | 1 | 99 | 2.52e-54 | 175 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0500680 | G7J6C6 | 71.429 | 126 | 9 | 1 | 1 | 126 | 1 | 99 | 1.20e-54 | 175 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0132550 | Msa0500680 | 0.836353 | 9.836976e-57 | -8.615850e-47 |
| Msa0500680 | Msa0887620 | 0.825055 | 5.774106e-54 | -8.615850e-47 |
| Msa0500680 | Msa1015930 | 0.801831 | 7.476968e-49 | -8.615850e-47 |
| Msa0500680 | Msa1430360 | 0.813664 | 2.282174e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0500680 | MtrunA17_Chr3g0133831 | 71.429 | 126 | 9 | 1 | 1 | 126 | 1 | 99 | 2.31e-58 | 175 |
| Msa0500680 | MtrunA17_Chr3g0133841 | 58.537 | 123 | 23 | 1 | 1 | 123 | 1 | 95 | 6.96e-43 | 136 |
| Msa0500680 | MtrunA17_Chr1g0153641 | 58.537 | 82 | 34 | 0 | 42 | 123 | 12 | 93 | 1.51e-30 | 105 |
| Msa0500680 | MtrunA17_Chr1g0153631 | 57.317 | 82 | 35 | 0 | 42 | 123 | 12 | 93 | 2.67e-30 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGGACTCTTTGTCTCTT+GGG | 0.376588 | 3_4:-41235127 | Msa0500680:CDS |
| CAAGAGGACTCTTTGTCTCT+TGG | 0.386912 | 3_4:-41235128 | Msa0500680:CDS |
| AAATTCAGGTATGCCGTTCA+TGG | 0.405667 | 3_4:-41235373 | None:intergenic |
| CGGTGGAGCAATTCAAACTA+TGG | 0.422285 | 3_4:-41235224 | Msa0500680:CDS |
| TGTCAAAATCAAACAATCCT+AGG | 0.458275 | 3_4:-41235004 | None:intergenic |
| GGGTTCAGTGATCATCATCT+TGG | 0.489219 | 3_4:-41235342 | Msa0500680:CDS |
| GGTTCAGTGATCATCATCTT+GGG | 0.490951 | 3_4:-41235341 | Msa0500680:CDS |
| AGACAAAGAGTCCTCTTGTA+TGG | 0.501353 | 3_4:+41235133 | None:intergenic |
| GAATTTGACACAAGCAATGA+TGG | 0.502729 | 3_4:-41235192 | Msa0500680:CDS |
| GTGTCTGCAGCGTAAAAGTC+AGG | 0.503300 | 3_4:+41235096 | None:intergenic |
| TGCCGTTCATGGAGCACAAA+GGG | 0.513317 | 3_4:-41235362 | Msa0500680:CDS |
| CTCAGTTGAATGATCGTCAT+GGG | 0.514849 | 3_4:-41235260 | Msa0500680:CDS |
| ATCAATGAAACCGTTATGAT+TGG | 0.516247 | 3_4:+41235074 | None:intergenic |
| AACCCTTTGTGCTCCATGAA+CGG | 0.532058 | 3_4:+41235360 | None:intergenic |
| GCTTGTCTGCAAAGTGAACA+AGG | 0.563870 | 3_4:+41235034 | None:intergenic |
| AGAGGACTCTTTGTCTCTTG+GGG | 0.566715 | 3_4:-41235126 | Msa0500680:CDS |
| CATGCTGTGCGCCATACAAG+AGG | 0.583294 | 3_4:-41235144 | Msa0500680:CDS |
| AACTGAGAAACGTGTGATGA+TGG | 0.584393 | 3_4:+41235276 | None:intergenic |
| GCTGCAGACACCAATCATAA+CGG | 0.590481 | 3_4:-41235084 | Msa0500680:CDS |
| TCTCAGTTGAATGATCGTCA+TGG | 0.593062 | 3_4:-41235261 | Msa0500680:CDS |
| TCATGGGAAGCACGTGATGA+CGG | 0.598511 | 3_4:-41235244 | Msa0500680:CDS |
| TGTGATGATGGTGATGGAGT+CGG | 0.625434 | 3_4:+41235288 | None:intergenic |
| GAAACGTGTGATGATGGTGA+TGG | 0.634663 | 3_4:+41235282 | None:intergenic |
| ATGCCGTTCATGGAGCACAA+AGG | 0.642777 | 3_4:-41235363 | Msa0500680:CDS |
| TGATGGCAAAATCAGCAAAG+AGG | 0.664740 | 3_4:-41235175 | Msa0500680:CDS |
| TGGGAAGCACGTGATGACGG+TGG | 0.691060 | 3_4:-41235241 | Msa0500680:CDS |
| TGGGAAACATGTGACAACTG+TGG | 0.741341 | 3_4:-41235322 | Msa0500680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAATGAAACCGTTATGAT+TGG | + | chr3_4:41235297-41235316 | None:intergenic | 30.0% | |
| GAATTTGACACAAGCAATGA+TGG | - | chr3_4:41235176-41235195 | Msa0500680:CDS | 35.0% | |
| GGTTCAGTGATCATCATCTT+GGG | - | chr3_4:41235027-41235046 | Msa0500680:CDS | 40.0% | |
| AACTGAGAAACGTGTGATGA+TGG | + | chr3_4:41235095-41235114 | None:intergenic | 40.0% | |
| TCTCAGTTGAATGATCGTCA+TGG | - | chr3_4:41235107-41235126 | Msa0500680:CDS | 40.0% | |
| CTCAGTTGAATGATCGTCAT+GGG | - | chr3_4:41235108-41235127 | Msa0500680:CDS | 40.0% | |
| TGATGGCAAAATCAGCAAAG+AGG | - | chr3_4:41235193-41235212 | Msa0500680:CDS | 40.0% | |
| AGACAAAGAGTCCTCTTGTA+TGG | + | chr3_4:41235238-41235257 | None:intergenic | 40.0% | |
| AAGAGGACTCTTTGTCTCTT+GGG | - | chr3_4:41235241-41235260 | Msa0500680:CDS | 40.0% | |
| AACCCTTTGTGCTCCATGAA+CGG | + | chr3_4:41235011-41235030 | None:intergenic | 45.0% | |
| GGGTTCAGTGATCATCATCT+TGG | - | chr3_4:41235026-41235045 | Msa0500680:CDS | 45.0% | |
| TGGGAAACATGTGACAACTG+TGG | - | chr3_4:41235046-41235065 | Msa0500680:CDS | 45.0% | |
| !! | TGTGATGATGGTGATGGAGT+CGG | + | chr3_4:41235083-41235102 | None:intergenic | 45.0% |
| !! | GAAACGTGTGATGATGGTGA+TGG | + | chr3_4:41235089-41235108 | None:intergenic | 45.0% |
| CGGTGGAGCAATTCAAACTA+TGG | - | chr3_4:41235144-41235163 | Msa0500680:CDS | 45.0% | |
| CAAGAGGACTCTTTGTCTCT+TGG | - | chr3_4:41235240-41235259 | Msa0500680:CDS | 45.0% | |
| AGAGGACTCTTTGTCTCTTG+GGG | - | chr3_4:41235242-41235261 | Msa0500680:CDS | 45.0% | |
| GCTGCAGACACCAATCATAA+CGG | - | chr3_4:41235284-41235303 | Msa0500680:CDS | 45.0% | |
| GCTTGTCTGCAAAGTGAACA+AGG | + | chr3_4:41235337-41235356 | None:intergenic | 45.0% | |
| ATGCCGTTCATGGAGCACAA+AGG | - | chr3_4:41235005-41235024 | Msa0500680:CDS | 50.0% | |
| TGCCGTTCATGGAGCACAAA+GGG | - | chr3_4:41235006-41235025 | Msa0500680:CDS | 50.0% | |
| ! | TCATGGGAAGCACGTGATGA+CGG | - | chr3_4:41235124-41235143 | Msa0500680:CDS | 50.0% |
| GTGTCTGCAGCGTAAAAGTC+AGG | + | chr3_4:41235275-41235294 | None:intergenic | 50.0% | |
| CATGCTGTGCGCCATACAAG+AGG | - | chr3_4:41235224-41235243 | Msa0500680:CDS | 55.0% | |
| !! | TGGGAAGCACGTGATGACGG+TGG | - | chr3_4:41235127-41235146 | Msa0500680:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 41235005 | 41235385 | 41235005 | ID=Msa0500680;Name=Msa0500680 |
| chr3_4 | mRNA | 41235005 | 41235385 | 41235005 | ID=Msa0500680-mRNA-1;Parent=Msa0500680;Name=Msa0500680-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|126 |
| chr3_4 | exon | 41235005 | 41235385 | 41235005 | ID=Msa0500680-mRNA-1:exon:8582;Parent=Msa0500680-mRNA-1 |
| chr3_4 | CDS | 41235005 | 41235385 | 41235005 | ID=Msa0500680-mRNA-1:cds;Parent=Msa0500680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
