AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1430360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430360 MtrunA17_Chr4g0046001 97.949 195 4 0 1 195 10 204 3.89e-141 399
Msa1430360 MtrunA17_Chr4g0046011 91.282 195 17 0 1 195 1 195 6.81e-134 380
Msa1430360 MtrunA17_Chr2g0283561 59.184 196 76 2 3 195 38 232 2.06e-81 248
Msa1430360 MtrunA17_Chr4g0039301 40.306 196 108 4 1 195 97 284 1.52e-45 157
Msa1430360 MtrunA17_Chr4g0041311 38.426 216 104 4 6 195 30 242 4.66e-44 151
Msa1430360 MtrunA17_Chr7g0260681 40.331 181 103 3 4 183 74 250 5.69e-43 148
Msa1430360 MtrunA17_Chr7g0260671 40.415 193 110 3 4 195 74 262 1.81e-42 148
Msa1430360 MtrunA17_Chr7g0214711 39.196 199 92 5 4 195 105 281 2.73e-38 137
Msa1430360 MtrunA17_Chr7g0250611 36.316 190 102 6 3 182 74 254 1.06e-27 107
Msa1430360 MtrunA17_Chr7g0250561 33.810 210 113 8 4 195 69 270 1.51e-25 102
Msa1430360 MtrunA17_Chr4g0055691 32.979 188 111 5 2 187 127 301 3.01e-22 93.2
Msa1430360 MtrunA17_Chr5g0399641 33.854 192 108 6 4 187 135 315 3.48e-22 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430360 AT4G18550.3 53.368 193 87 3 5 195 37 228 1.08e-65 204
Msa1430360 AT4G18550.1 53.368 193 87 3 5 195 20 211 3.36e-64 204
Msa1430360 AT4G18550.2 53.368 193 87 3 5 195 37 228 4.29e-64 204
Msa1430360 AT1G06250.1 48.205 195 97 2 4 195 5 198 1.21e-63 202
Msa1430360 AT1G51440.1 41.837 196 103 5 3 195 86 273 1.99e-44 154
Msa1430360 AT2G30550.1 39.698 199 104 5 4 195 101 290 4.40e-42 147
Msa1430360 AT2G30550.2 39.698 199 104 5 4 195 101 290 1.80e-41 146
Msa1430360 AT2G42690.1 45.238 168 88 3 1 167 1 165 1.84e-40 142
Msa1430360 AT1G30370.1 37.056 197 110 4 4 195 114 301 1.73e-39 141
Msa1430360 AT1G06800.2 38.500 200 106 6 4 195 85 275 2.13e-39 140
Msa1430360 AT1G06800.1 38.500 200 106 6 4 195 85 275 6.72e-39 139
Msa1430360 AT2G31690.1 35.233 193 116 3 4 195 88 272 5.48e-36 131
Msa1430360 AT1G05800.1 35.751 193 112 4 4 195 80 261 5.28e-33 123
Msa1430360 AT2G44810.2 36.216 185 102 5 4 180 86 262 4.13e-29 112
Msa1430360 AT2G44810.1 36.872 179 97 5 10 180 2 172 1.11e-27 107
Msa1430360 AT4G16820.1 36.316 190 97 8 4 187 127 298 2.38e-24 99.8
Msa1430360 AT1G06250.2 48.810 84 40 1 115 195 1 84 1.79e-19 84.3

Find 44 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGTTGCATACAAATATT+TGG 0.233512 tig0026647:-63375 None:intergenic
TAATTGTCGTTATTCTAAAA+AGG 0.269491 tig0026647:+63298 Msa1430360:CDS
GATCCTGCACCACTAATATT+TGG 0.272052 tig0026647:+63763 Msa1430360:CDS
CGTAAGTACTTACTACATTA+TGG 0.283055 tig0026647:+63215 Msa1430360:CDS
AAGTAAGTGGAAAGGTTTAT+TGG 0.289889 tig0026647:+63172 Msa1430360:CDS
TTCGAAGGATGCTTGGAGTA+TGG 0.324277 tig0026647:+63439 Msa1430360:CDS
GGCTGCACTATCACTTGCTT+TGG 0.336091 tig0026647:-63396 None:intergenic
TGTATTTGTACATTTGATTC+CGG 0.336182 tig0026647:-63787 None:intergenic
TGTTTGGAGAGGAACAATTC+AGG 0.355595 tig0026647:+63705 Msa1430360:CDS
CTTGGAGTATGGAATCAAAT+TGG 0.367290 tig0026647:+63450 Msa1430360:CDS
TTGGTCACAGAATACTTATA+AGG 0.371532 tig0026647:-63356 None:intergenic
GGAGGGACATAGTCGTTGTT+TGG 0.383794 tig0026647:+63689 Msa1430360:CDS
ACCTTCTTCCAAATATACTT+TGG 0.398759 tig0026647:-63330 None:intergenic
TCCAAAGTATATTTGGAAGA+AGG 0.409048 tig0026647:+63329 Msa1430360:CDS
TGTAACAGCTAAGGAGGCCT+TGG 0.418766 tig0026647:+63663 Msa1430360:intron
CTAAGGAGGCCTTGGGGAGG+AGG 0.419172 tig0026647:+63671 Msa1430360:CDS
TGATTCTAGCGCTAGAAAAC+AGG 0.427368 tig0026647:+63861 Msa1430360:CDS
GATTCCGGACCAAATATTAG+TGG 0.445624 tig0026647:-63772 None:intergenic
GTAACAGCTAAGGAGGCCTT+GGG 0.455565 tig0026647:+63664 Msa1430360:intron
TGCACCACTAATATTTGGTC+CGG 0.502264 tig0026647:+63768 Msa1430360:CDS
GTTTGGAGAGGAACAATTCA+GGG 0.508947 tig0026647:+63706 Msa1430360:CDS
GGACCAAATATTAGTGGTGC+AGG 0.511094 tig0026647:-63766 None:intergenic
AAATATTGAAGTTAAAAGAA+AGG 0.512857 tig0026647:-63417 None:intergenic
GAGTATGGAATCAAATTGGA+TGG 0.512934 tig0026647:+63454 Msa1430360:CDS
TAAGGAGGCCTTGGGGAGGA+GGG 0.516311 tig0026647:+63672 Msa1430360:CDS
AACTTAGTGGCAAAAGTAAG+TGG 0.534655 tig0026647:+63159 Msa1430360:CDS
CTTTACACATCTGAAAACTC+AGG 0.534710 tig0026647:+63826 Msa1430360:CDS
AACAATTCAGGGTTCTGAGT+GGG 0.560505 tig0026647:+63717 Msa1430360:CDS
AGTATGGAATCAAATTGGAT+GGG 0.577722 tig0026647:+63455 Msa1430360:CDS
AAAACATGGAGAGAACTTAG+TGG 0.589573 tig0026647:+63146 Msa1430360:CDS
ATTATATGTAACAGCTAAGG+AGG 0.589612 tig0026647:+63657 Msa1430360:intron
GACATAGTCGTTGTTTGGAG+AGG 0.592838 tig0026647:+63694 Msa1430360:CDS
TTACGTAGGTCAATGTGTAA+AGG 0.596130 tig0026647:-63197 None:intergenic
CTAGCGCTAGAATCATCTGA+TGG 0.596192 tig0026647:-63853 None:intergenic
GACTATGTCCCTCCTCCCCA+AGG 0.598380 tig0026647:-63680 None:intergenic
AGTGGCAAAAGTAAGTGGAA+AGG 0.602358 tig0026647:+63164 Msa1430360:CDS
GTATGGAATCAAATTGGATG+GGG 0.608289 tig0026647:+63456 Msa1430360:CDS
CTCAACATTACCTTCGTCAG+TGG 0.608844 tig0026647:-63492 None:intergenic
CAGCTAAGGAGGCCTTGGGG+AGG 0.642109 tig0026647:+63668 Msa1430360:intron
GTTGCTGTTGCCACTGACGA+AGG 0.677744 tig0026647:+63482 Msa1430360:CDS
GAACAATTCAGGGTTCTGAG+TGG 0.692237 tig0026647:+63716 Msa1430360:CDS
AATGTAGTAAGTACTTACGT+AGG 0.694414 tig0026647:-63211 None:intergenic
TCAAATGTACAAATACACAA+CGG 0.709239 tig0026647:+63793 Msa1430360:CDS
TAACAGCTAAGGAGGCCTTG+GGG 0.746795 tig0026647:+63665 Msa1430360:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAAATTTTAATATTGATT+TGG + tig0026647:63741-63760 Msa1430360:CDS 10.0%
!!! TCAAAATTTTAATATTGATT+TGG + tig0026647:63741-63760 Msa1430360:CDS 10.0%
!! AAATATTGAAGTTAAAAGAA+AGG - tig0026647:63420-63439 None:intergenic 15.0%
!! AAAAATAGAAACAAAAATTG+CGG + tig0026647:63524-63543 Msa1430360:intron 15.0%
!! AAATATTGAAGTTAAAAGAA+AGG - tig0026647:63420-63439 None:intergenic 15.0%
!! AAAAATAGAAACAAAAATTG+CGG + tig0026647:63524-63543 Msa1430360:intron 15.0%
!!! TAATTGTCGTTATTCTAAAA+AGG + tig0026647:63298-63317 Msa1430360:CDS 20.0%
!!! TTTCTTTTCCAAAGTATATT+TGG + tig0026647:63322-63341 Msa1430360:CDS 20.0%
!!! TTTAACTTCAATATTTTCGA+AGG + tig0026647:63424-63443 Msa1430360:CDS 20.0%
!! AAAATAGAAACAAAAATTGC+GGG + tig0026647:63525-63544 Msa1430360:intron 20.0%
!!! TAATTGTCGTTATTCTAAAA+AGG + tig0026647:63298-63317 Msa1430360:CDS 20.0%
!!! TTTCTTTTCCAAAGTATATT+TGG + tig0026647:63322-63341 Msa1430360:CDS 20.0%
!!! TTTAACTTCAATATTTTCGA+AGG + tig0026647:63424-63443 Msa1430360:CDS 20.0%
!! AAAATAGAAACAAAAATTGC+GGG + tig0026647:63525-63544 Msa1430360:intron 20.0%
! ACAATTATATGTAACAGCTA+AGG + tig0026647:63654-63673 Msa1430360:intron 25.0%
! TGTATTTGTACATTTGATTC+CGG - tig0026647:63790-63809 None:intergenic 25.0%
! TCAAATGTACAAATACACAA+CGG + tig0026647:63793-63812 Msa1430360:CDS 25.0%
! ACAATTATATGTAACAGCTA+AGG + tig0026647:63654-63673 Msa1430360:intron 25.0%
! TGTATTTGTACATTTGATTC+CGG - tig0026647:63790-63809 None:intergenic 25.0%
! TCAAATGTACAAATACACAA+CGG + tig0026647:63793-63812 Msa1430360:CDS 25.0%
AAGTAAGTGGAAAGGTTTAT+TGG + tig0026647:63172-63191 Msa1430360:CDS 30.0%
AATGTAGTAAGTACTTACGT+AGG - tig0026647:63214-63233 None:intergenic 30.0%
CGTAAGTACTTACTACATTA+TGG + tig0026647:63215-63234 Msa1430360:CDS 30.0%
TCCAAAGTATATTTGGAAGA+AGG + tig0026647:63329-63348 Msa1430360:CDS 30.0%
ACCTTCTTCCAAATATACTT+TGG - tig0026647:63333-63352 None:intergenic 30.0%
TTGGTCACAGAATACTTATA+AGG - tig0026647:63359-63378 None:intergenic 30.0%
GGATGTTGCATACAAATATT+TGG - tig0026647:63378-63397 None:intergenic 30.0%
AGTATGGAATCAAATTGGAT+GGG + tig0026647:63455-63474 Msa1430360:CDS 30.0%
ATTATATGTAACAGCTAAGG+AGG + tig0026647:63657-63676 Msa1430360:intron 30.0%
! TGAGTTTTCAGATGTGTAAA+GGG - tig0026647:63827-63846 None:intergenic 30.0%
AAGTAAGTGGAAAGGTTTAT+TGG + tig0026647:63172-63191 Msa1430360:CDS 30.0%
AATGTAGTAAGTACTTACGT+AGG - tig0026647:63214-63233 None:intergenic 30.0%
CGTAAGTACTTACTACATTA+TGG + tig0026647:63215-63234 Msa1430360:CDS 30.0%
TCCAAAGTATATTTGGAAGA+AGG + tig0026647:63329-63348 Msa1430360:CDS 30.0%
ACCTTCTTCCAAATATACTT+TGG - tig0026647:63333-63352 None:intergenic 30.0%
TTGGTCACAGAATACTTATA+AGG - tig0026647:63359-63378 None:intergenic 30.0%
GGATGTTGCATACAAATATT+TGG - tig0026647:63378-63397 None:intergenic 30.0%
AGTATGGAATCAAATTGGAT+GGG + tig0026647:63455-63474 Msa1430360:CDS 30.0%
ATTATATGTAACAGCTAAGG+AGG + tig0026647:63657-63676 Msa1430360:intron 30.0%
! TGAGTTTTCAGATGTGTAAA+GGG - tig0026647:63827-63846 None:intergenic 30.0%
AAAACATGGAGAGAACTTAG+TGG + tig0026647:63146-63165 Msa1430360:CDS 35.0%
AACTTAGTGGCAAAAGTAAG+TGG + tig0026647:63159-63178 Msa1430360:CDS 35.0%
TTACGTAGGTCAATGTGTAA+AGG - tig0026647:63200-63219 None:intergenic 35.0%
!! GAAAAAGCATCTAAGTATGC+AGG + tig0026647:63275-63294 Msa1430360:CDS 35.0%
! CAATATTTTCGAAGGATGCT+TGG + tig0026647:63432-63451 Msa1430360:CDS 35.0%
CTTGGAGTATGGAATCAAAT+TGG + tig0026647:63450-63469 Msa1430360:CDS 35.0%
GAGTATGGAATCAAATTGGA+TGG + tig0026647:63454-63473 Msa1430360:CDS 35.0%
GTATGGAATCAAATTGGATG+GGG + tig0026647:63456-63475 Msa1430360:CDS 35.0%
! CTGAGTTTTCAGATGTGTAA+AGG - tig0026647:63828-63847 None:intergenic 35.0%
CTTTACACATCTGAAAACTC+AGG + tig0026647:63826-63845 Msa1430360:CDS 35.0%
AAAACATGGAGAGAACTTAG+TGG + tig0026647:63146-63165 Msa1430360:CDS 35.0%
AACTTAGTGGCAAAAGTAAG+TGG + tig0026647:63159-63178 Msa1430360:CDS 35.0%
TTACGTAGGTCAATGTGTAA+AGG - tig0026647:63200-63219 None:intergenic 35.0%
!! GAAAAAGCATCTAAGTATGC+AGG + tig0026647:63275-63294 Msa1430360:CDS 35.0%
! CAATATTTTCGAAGGATGCT+TGG + tig0026647:63432-63451 Msa1430360:CDS 35.0%
CTTGGAGTATGGAATCAAAT+TGG + tig0026647:63450-63469 Msa1430360:CDS 35.0%
GAGTATGGAATCAAATTGGA+TGG + tig0026647:63454-63473 Msa1430360:CDS 35.0%
GTATGGAATCAAATTGGATG+GGG + tig0026647:63456-63475 Msa1430360:CDS 35.0%
! CTGAGTTTTCAGATGTGTAA+AGG - tig0026647:63828-63847 None:intergenic 35.0%
CTTTACACATCTGAAAACTC+AGG + tig0026647:63826-63845 Msa1430360:CDS 35.0%
TGGGTAGCATAGCAAAAACA+TGG + tig0026647:63132-63151 Msa1430360:CDS 40.0%
AGTGGCAAAAGTAAGTGGAA+AGG + tig0026647:63164-63183 Msa1430360:CDS 40.0%
TGTTTGGAGAGGAACAATTC+AGG + tig0026647:63705-63724 Msa1430360:CDS 40.0%
GTTTGGAGAGGAACAATTCA+GGG + tig0026647:63706-63725 Msa1430360:CDS 40.0%
!! AACAATTCAGGGTTCTGAGT+GGG + tig0026647:63717-63736 Msa1430360:CDS 40.0%
GATCCTGCACCACTAATATT+TGG + tig0026647:63763-63782 Msa1430360:CDS 40.0%
TGCACCACTAATATTTGGTC+CGG + tig0026647:63768-63787 Msa1430360:CDS 40.0%
GATTCCGGACCAAATATTAG+TGG - tig0026647:63775-63794 None:intergenic 40.0%
TGGGTAGCATAGCAAAAACA+TGG + tig0026647:63132-63151 Msa1430360:CDS 40.0%
AGTGGCAAAAGTAAGTGGAA+AGG + tig0026647:63164-63183 Msa1430360:CDS 40.0%
TGTTTGGAGAGGAACAATTC+AGG + tig0026647:63705-63724 Msa1430360:CDS 40.0%
GTTTGGAGAGGAACAATTCA+GGG + tig0026647:63706-63725 Msa1430360:CDS 40.0%
!! AACAATTCAGGGTTCTGAGT+GGG + tig0026647:63717-63736 Msa1430360:CDS 40.0%
GATCCTGCACCACTAATATT+TGG + tig0026647:63763-63782 Msa1430360:CDS 40.0%
TGCACCACTAATATTTGGTC+CGG + tig0026647:63768-63787 Msa1430360:CDS 40.0%
GATTCCGGACCAAATATTAG+TGG - tig0026647:63775-63794 None:intergenic 40.0%
TTCGAAGGATGCTTGGAGTA+TGG + tig0026647:63439-63458 Msa1430360:CDS 45.0%
CTCAACATTACCTTCGTCAG+TGG - tig0026647:63495-63514 None:intergenic 45.0%
GACATAGTCGTTGTTTGGAG+AGG + tig0026647:63694-63713 Msa1430360:CDS 45.0%
!! GAACAATTCAGGGTTCTGAG+TGG + tig0026647:63716-63735 Msa1430360:CDS 45.0%
GGACCAAATATTAGTGGTGC+AGG - tig0026647:63769-63788 None:intergenic 45.0%
CTAGCGCTAGAATCATCTGA+TGG - tig0026647:63856-63875 None:intergenic 45.0%
TTCGAAGGATGCTTGGAGTA+TGG + tig0026647:63439-63458 Msa1430360:CDS 45.0%
CTCAACATTACCTTCGTCAG+TGG - tig0026647:63495-63514 None:intergenic 45.0%
GACATAGTCGTTGTTTGGAG+AGG + tig0026647:63694-63713 Msa1430360:CDS 45.0%
!! GAACAATTCAGGGTTCTGAG+TGG + tig0026647:63716-63735 Msa1430360:CDS 45.0%
GGACCAAATATTAGTGGTGC+AGG - tig0026647:63769-63788 None:intergenic 45.0%
CTAGCGCTAGAATCATCTGA+TGG - tig0026647:63856-63875 None:intergenic 45.0%
GGCTGCACTATCACTTGCTT+TGG - tig0026647:63399-63418 None:intergenic 50.0%
TGTAACAGCTAAGGAGGCCT+TGG + tig0026647:63663-63682 Msa1430360:intron 50.0%
GTAACAGCTAAGGAGGCCTT+GGG + tig0026647:63664-63683 Msa1430360:intron 50.0%
TAACAGCTAAGGAGGCCTTG+GGG + tig0026647:63665-63684 Msa1430360:intron 50.0%
GGAGGGACATAGTCGTTGTT+TGG + tig0026647:63689-63708 Msa1430360:CDS 50.0%
GGCTGCACTATCACTTGCTT+TGG - tig0026647:63399-63418 None:intergenic 50.0%
TGTAACAGCTAAGGAGGCCT+TGG + tig0026647:63663-63682 Msa1430360:intron 50.0%
GTAACAGCTAAGGAGGCCTT+GGG + tig0026647:63664-63683 Msa1430360:intron 50.0%
TAACAGCTAAGGAGGCCTTG+GGG + tig0026647:63665-63684 Msa1430360:intron 50.0%
GGAGGGACATAGTCGTTGTT+TGG + tig0026647:63689-63708 Msa1430360:CDS 50.0%
! GTTGCTGTTGCCACTGACGA+AGG + tig0026647:63482-63501 Msa1430360:CDS 55.0%
! GTTGCTGTTGCCACTGACGA+AGG + tig0026647:63482-63501 Msa1430360:CDS 55.0%
TAAGGAGGCCTTGGGGAGGA+GGG + tig0026647:63672-63691 Msa1430360:CDS 60.0%
GACTATGTCCCTCCTCCCCA+AGG - tig0026647:63683-63702 None:intergenic 60.0%
TAAGGAGGCCTTGGGGAGGA+GGG + tig0026647:63672-63691 Msa1430360:CDS 60.0%
GACTATGTCCCTCCTCCCCA+AGG - tig0026647:63683-63702 None:intergenic 60.0%
CAGCTAAGGAGGCCTTGGGG+AGG + tig0026647:63668-63687 Msa1430360:intron 65.0%
CTAAGGAGGCCTTGGGGAGG+AGG + tig0026647:63671-63690 Msa1430360:CDS 65.0%
CAGCTAAGGAGGCCTTGGGG+AGG + tig0026647:63668-63687 Msa1430360:intron 65.0%
CTAAGGAGGCCTTGGGGAGG+AGG + tig0026647:63671-63690 Msa1430360:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0026647 gene 63131 63882 63131 ID=Msa1430360;Name=Msa1430360
tig0026647 mRNA 63131 63882 63131 ID=Msa1430360-mRNA-1;Parent=Msa1430360;Name=Msa1430360-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|195
tig0026647 exon 63131 63503 63131 ID=Msa1430360-mRNA-1:exon:6961;Parent=Msa1430360-mRNA-1
tig0026647 exon 63671 63882 63671 ID=Msa1430360-mRNA-1:exon:6962;Parent=Msa1430360-mRNA-1
tig0026647 CDS 63131 63503 63131 ID=Msa1430360-mRNA-1:cds;Parent=Msa1430360-mRNA-1
tig0026647 CDS 63671 63882 63671 ID=Msa1430360-mRNA-1:cds;Parent=Msa1430360-mRNA-1
Gene Sequence

>Msa1430360

ATGGGTAGCATAGCAAAAACATGGAGAGAACTTAGTGGCAAAAGTAAGTGGAAAGGTTTATTGGAACCTTTACACATTGACCTACGTAAGTACTTACTACATTATGGACAATTTGCTCAAGCTACATATGATGCTTTCAACTTTGAAAAAGCATCTAAGTATGCAGGTAATTGTCGTTATTCTAAAAAGGATTTCTTTTCCAAAGTATATTTGGAAGAAGGTAATCCTTATAAGTATTCTGTGACCAAATATTTGTATGCAACATCCAAAGCAAGTGATAGTGCAGCCTTTCTTTTAACTTCAATATTTTCGAAGGATGCTTGGAGTATGGAATCAAATTGGATGGGGTATGTTGCTGTTGCCACTGACGAAGCTAAGGAGGCCTTGGGGAGGAGGGACATAGTCGTTGTTTGGAGAGGAACAATTCAGGGTTCTGAGTGGGTTCAAAATTTTAATATTGATTTGGATCCTGCACCACTAATATTTGGTCCGGAATCAAATGTACAAATACACAACGGATTTTACTCCCTTTACACATCTGAAAACTCAGGTTTACCATCAGATGATTCTAGCGCTAGAAAACAG

Protein sequence

>Msa1430360

MGSIAKTWRELSGKSKWKGLLEPLHIDLRKYLLHYGQFAQATYDAFNFEKASKYAGNCRYSKKDFFSKVYLEEGNPYKYSVTKYLYATSKASDSAAFLLTSIFSKDAWSMESNWMGYVAVATDEAKEALGRRDIVVVWRGTIQGSEWVQNFNIDLDPAPLIFGPESNVQIHNGFYSLYTSENSGLPSDDSSARKQ