AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0523820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523820 MtrunA17_Chr3g0144641 86.424 302 26 5 1 299 1 290 0.0 518
Msa0523820 MtrunA17_Chr4g0035101 52.759 290 68 12 1 283 1 228 8.52e-78 241
Msa0523820 MtrunA17_Chr7g0234761 59.302 172 64 4 114 283 235 402 1.18e-51 178
Msa0523820 MtrunA17_Chr8g0365941 56.364 165 55 2 132 283 230 390 1.21e-51 177
Msa0523820 MtrunA17_Chr5g0421111 55.080 187 64 5 132 302 290 472 1.28e-51 179
Msa0523820 MtrunA17_Chr1g0152131 47.208 197 60 3 150 305 215 408 1.56e-49 170
Msa0523820 MtrunA17_Chr8g0339091 57.229 166 67 2 114 279 211 372 2.34e-48 169
Msa0523820 MtrunA17_Chr5g0415291 63.934 122 35 2 150 271 73 185 1.26e-42 147
Msa0523820 MtrunA17_Chr3g0131771 68.519 108 31 1 182 286 131 238 2.61e-42 149
Msa0523820 MtrunA17_Chr8g0363921 81.928 83 15 0 195 277 148 230 8.60e-42 146
Msa0523820 MtrunA17_Chr8g0385911 82.558 86 14 1 193 277 140 225 1.00e-41 147
Msa0523820 MtrunA17_Chr7g0258161 65.421 107 29 1 174 280 157 255 1.19e-41 147
Msa0523820 MtrunA17_Chr1g0174161 64.151 106 33 1 191 291 153 258 3.44e-40 143
Msa0523820 MtrunA17_Chr6g0484271 69.318 88 25 1 194 279 151 238 7.06e-34 125
Msa0523820 MtrunA17_Chr4g0074161 72.727 66 17 1 198 263 206 270 8.83e-23 97.8
Msa0523820 MtrunA17_Chr1g0190731 69.697 66 19 1 198 263 193 257 1.11e-21 94.0
Msa0523820 MtrunA17_Chr4g0032951 58.462 65 27 0 198 262 355 419 7.47e-16 77.8
Msa0523820 MtrunA17_Chr4g0014771 55.385 65 29 0 198 262 118 182 1.62e-15 73.9
Msa0523820 MtrunA17_Chr1g0182791 44.944 89 40 2 200 288 319 398 1.79e-15 77.0
Msa0523820 MtrunA17_Chr2g0329041 55.385 65 29 0 196 260 330 394 2.29e-15 76.3
Msa0523820 MtrunA17_Chr1g0193321 45.205 73 39 1 200 272 464 535 6.29e-15 75.5
Msa0523820 MtrunA17_Chr1g0174271 53.623 69 32 0 198 266 298 366 8.44e-15 74.3
Msa0523820 MtrunA17_Chr1g0184841 65.152 66 22 1 198 263 120 184 9.73e-15 73.2
Msa0523820 MtrunA17_Chr7g0258181 52.174 69 33 0 198 266 243 311 1.35e-14 73.2
Msa0523820 MtrunA17_Chr3g0087771 46.753 77 41 0 186 262 310 386 2.74e-13 70.1
Msa0523820 MtrunA17_Chr3g0134741 35.032 157 92 4 133 288 172 319 7.93e-13 68.2
Msa0523820 MtrunA17_Chr8g0351881 49.231 65 33 0 198 262 316 380 1.34e-12 67.8
Msa0523820 MtrunA17_Chr1g0152651 44.828 87 45 2 176 262 254 337 1.75e-12 67.4
Msa0523820 MtrunA17_Chr1g0153241 47.692 65 33 1 200 264 87 150 2.04e-12 66.2
Msa0523820 MtrunA17_Chr7g0262691 40.449 89 48 3 201 288 255 339 9.31e-12 65.5
Msa0523820 MtrunA17_Chr3g0095391 50.000 64 31 1 200 263 311 373 9.58e-12 65.5
Msa0523820 MtrunA17_Chr7g0270871 43.939 66 36 1 209 274 213 277 2.97e-11 63.9
Msa0523820 MtrunA17_Chr5g0393721 50.000 64 31 1 199 262 225 287 3.22e-11 63.5
Msa0523820 MtrunA17_Chr7g0271251 46.053 76 38 2 190 263 422 496 3.23e-11 64.3
Msa0523820 MtrunA17_Chr5g0402031 53.571 56 25 1 209 264 116 170 4.32e-11 63.2
Msa0523820 MtrunA17_Chr1g0213791 48.438 64 33 0 198 261 265 328 6.04e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523820 AT4G34530.1 64.935 154 49 4 142 291 113 265 6.12e-57 187
Msa0523820 AT4G36540.1 43.158 285 103 12 22 295 2 238 5.13e-52 174
Msa0523820 AT2G18300.2 58.788 165 51 5 122 285 124 272 1.03e-51 174
Msa0523820 AT2G18300.1 59.748 159 47 5 122 279 124 266 1.36e-51 174
Msa0523820 AT2G18300.3 58.788 165 51 5 122 285 124 272 2.77e-51 174
Msa0523820 AT4G36540.2 43.590 273 95 12 22 283 2 226 5.16e-51 171
Msa0523820 AT1G68920.1 69.167 120 36 1 165 283 269 388 3.18e-50 174
Msa0523820 AT1G68920.4 69.167 120 36 1 165 283 269 388 3.18e-50 174
Msa0523820 AT1G68920.2 69.167 120 36 1 165 283 269 388 3.18e-50 174
Msa0523820 AT1G68920.3 69.167 120 36 1 165 283 268 387 3.54e-50 174
Msa0523820 AT3G07340.2 82.474 97 15 1 194 290 254 348 4.22e-49 170
Msa0523820 AT3G07340.1 82.474 97 15 1 194 290 254 348 5.73e-49 170
Msa0523820 AT1G10120.2 51.724 174 43 2 165 304 180 346 9.48e-48 164
Msa0523820 AT1G10120.1 51.724 174 43 2 165 304 180 346 9.48e-48 164
Msa0523820 AT3G23690.1 64.179 134 41 4 148 277 140 270 2.73e-47 163
Msa0523820 AT5G48560.2 75.926 108 24 2 180 286 284 390 4.53e-47 165
Msa0523820 AT5G48560.1 75.926 108 24 2 180 286 284 390 1.02e-46 165
Msa0523820 AT1G26260.3 69.027 113 35 0 188 300 162 274 1.04e-46 161
Msa0523820 AT1G26260.5 69.027 113 35 0 188 300 191 303 2.06e-46 161
Msa0523820 AT1G26260.4 78.125 96 21 0 188 283 213 308 3.93e-46 160
Msa0523820 AT1G26260.1 78.125 96 21 0 188 283 213 308 3.93e-46 160
Msa0523820 AT1G26260.6 78.125 96 21 0 188 283 213 308 3.93e-46 160
Msa0523820 AT1G26260.2 78.125 96 21 0 188 283 213 308 3.93e-46 160
Msa0523820 AT1G59640.2 50.562 178 80 3 139 312 79 252 3.13e-43 152
Msa0523820 AT1G59640.1 54.839 155 62 3 139 289 79 229 4.35e-43 149
Msa0523820 AT5G50915.3 61.069 131 42 3 154 277 88 216 4.84e-43 149
Msa0523820 AT5G50915.4 61.069 131 42 3 154 277 88 216 4.84e-43 149
Msa0523820 AT5G50915.1 61.069 131 42 3 154 277 88 216 1.47e-42 149
Msa0523820 AT5G50915.2 61.069 131 42 3 154 277 88 216 1.47e-42 149
Msa0523820 AT2G42300.4 69.474 95 29 0 192 286 179 273 3.71e-42 147
Msa0523820 AT2G42300.1 60.000 115 45 1 192 305 179 293 1.31e-41 147
Msa0523820 AT3G57800.2 72.043 93 26 0 191 283 197 289 1.65e-41 148
Msa0523820 AT5G62610.2 56.923 130 48 2 141 269 102 224 1.28e-38 138
Msa0523820 AT5G62610.1 56.923 130 48 2 141 269 102 224 1.28e-38 138
Msa0523820 AT1G73830.2 50.704 142 61 1 134 275 92 224 5.43e-38 136
Msa0523820 AT1G73830.1 50.704 142 61 1 134 275 92 224 5.69e-38 136
Msa0523820 AT1G18400.2 75.000 80 20 0 196 275 143 222 1.02e-35 129
Msa0523820 AT1G18400.1 75.000 80 20 0 196 275 143 222 2.00e-35 129
Msa0523820 AT1G25330.1 68.605 86 27 0 194 279 100 185 1.75e-33 123
Msa0523820 AT3G57800.1 47.857 140 26 1 191 283 197 336 1.57e-32 125
Msa0523820 AT5G58010.1 65.000 80 23 2 198 273 99 177 7.31e-24 99.4
Msa0523820 AT2G24260.2 71.212 66 18 1 198 263 35 99 2.90e-23 96.7
Msa0523820 AT4G30980.1 72.727 66 17 1 198 263 130 194 5.48e-23 97.4
Msa0523820 AT4G30980.2 72.727 66 17 1 198 263 178 242 8.81e-23 97.4
Msa0523820 AT2G24260.1 71.212 66 18 1 198 263 138 202 1.95e-22 96.7
Msa0523820 AT2G42300.5 76.087 46 11 0 192 237 179 224 2.52e-18 82.8
Msa0523820 AT2G42300.3 76.087 46 11 0 192 237 179 224 2.52e-18 82.8
Msa0523820 AT2G42300.2 76.087 46 11 0 192 237 179 224 3.10e-18 82.8
Msa0523820 AT2G42280.3 37.984 129 58 4 144 261 227 344 3.97e-16 78.6
Msa0523820 AT2G42280.1 37.008 127 60 3 144 261 227 342 4.38e-16 78.6
Msa0523820 AT2G20180.7 42.553 94 48 2 170 263 184 271 1.61e-15 77.0
Msa0523820 AT2G20180.1 42.553 94 48 2 170 263 184 271 1.61e-15 77.0
Msa0523820 AT2G20180.5 42.553 94 48 2 170 263 184 271 1.61e-15 77.0
Msa0523820 AT2G20180.4 42.553 94 48 2 170 263 255 342 2.06e-15 77.0
Msa0523820 AT2G20180.6 42.553 94 48 2 170 263 255 342 2.06e-15 77.0
Msa0523820 AT2G20180.2 42.553 94 48 2 170 263 255 342 2.06e-15 77.0
Msa0523820 AT2G20180.3 42.553 94 48 2 170 263 255 342 2.06e-15 77.0
Msa0523820 AT4G28790.1 30.108 186 94 5 87 272 195 344 7.80e-15 75.1
Msa0523820 AT1G51140.1 56.250 64 28 0 198 261 304 367 1.64e-14 73.9
Msa0523820 AT1G03040.2 50.549 91 44 1 173 263 114 203 2.61e-14 72.8
Msa0523820 AT1G03040.1 60.274 73 28 1 191 263 137 208 3.44e-14 72.4
Msa0523820 AT4G09180.1 50.746 67 33 0 196 262 182 248 3.75e-14 71.6
Msa0523820 AT1G09530.3 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT1G09530.2 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT1G09530.5 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT1G09530.1 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT1G09530.4 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT1G09530.6 50.000 64 31 1 200 263 339 401 3.76e-14 73.6
Msa0523820 AT4G02590.3 63.636 66 23 1 198 263 83 147 4.55e-14 71.2
Msa0523820 AT1G03040.3 60.274 73 28 1 191 263 137 208 6.40e-14 72.0
Msa0523820 AT4G02590.2 63.636 66 23 1 198 263 146 210 6.48e-14 71.6
Msa0523820 AT4G02590.1 63.636 66 23 1 198 263 146 210 6.48e-14 71.6
Msa0523820 AT1G35460.1 52.308 65 31 0 198 262 181 245 6.58e-14 70.9
Msa0523820 AT2G20180.8 39.000 100 55 2 161 260 246 339 8.43e-14 71.6
Msa0523820 AT4G28815.2 56.897 58 24 1 201 258 71 127 9.40e-14 70.1
Msa0523820 AT4G28800.1 36.441 118 64 3 201 318 252 358 1.36e-13 71.6
Msa0523820 AT4G28815.1 56.897 58 24 1 201 258 147 203 3.22e-13 69.7
Msa0523820 AT4G28790.2 29.609 179 90 5 87 265 195 337 8.02e-13 68.6
Msa0523820 AT1G27740.1 38.679 106 62 2 157 262 128 230 2.31e-12 66.6
Msa0523820 AT4G33880.1 38.182 110 65 2 172 279 238 346 2.47e-12 67.4
Msa0523820 AT4G28800.2 52.459 61 28 1 201 261 264 323 3.93e-12 67.0
Msa0523820 AT2G46970.2 40.000 100 53 3 176 270 166 263 4.44e-12 66.6
Msa0523820 AT2G46970.3 40.000 100 53 3 176 270 175 272 4.57e-12 66.6
Msa0523820 AT2G46970.1 42.045 88 44 3 188 270 209 294 4.60e-12 67.0
Msa0523820 AT4G00050.2 50.769 65 31 1 198 262 105 168 1.93e-11 64.3
Msa0523820 AT2G43140.1 50.000 64 32 0 198 261 134 197 2.46e-11 62.8
Msa0523820 AT2G14760.1 39.286 112 60 3 157 262 191 300 2.68e-11 63.9
Msa0523820 AT2G43140.2 50.000 64 32 0 198 261 231 294 3.35e-11 63.5
Msa0523820 AT4G00050.1 50.769 65 31 1 198 262 207 270 3.50e-11 63.9
Msa0523820 AT4G36930.1 50.820 61 29 1 204 264 197 256 3.61e-11 63.9
Msa0523820 AT4G00050.3 50.769 65 31 1 198 262 207 270 3.88e-11 63.9

Find 115 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGCAACTTGGATTTAA+AGG 0.136324 3_4:-77268412 Msa0523820:three_prime_UTR
TTCACAAAATATAATTAATT+TGG 0.143404 3_4:+77268485 None:intergenic
TTACAACTTAGGGTTGTTTC+TGG 0.202689 3_4:-77268339 Msa0523820:three_prime_UTR
TTTCACAAGGCTTACGCTTA+TGG 0.213360 3_4:-77268260 Msa0523820:three_prime_UTR
TCACCTTCCCATGTTGTATT+AGG 0.244216 3_4:+77269305 None:intergenic
AATTGAAGTTGTATAGGTTT+TGG 0.253509 3_4:+77269279 None:intergenic
GAATGTTCCAACACTAAATA+TGG 0.259489 3_4:-77268104 Msa0523820:three_prime_UTR
TCTCAATCTCAGTTATTTAC+AGG 0.262036 3_4:-77269227 Msa0523820:CDS
TGTGACAGTGTTAGTGTCTT+TGG 0.270501 3_4:+77271001 None:intergenic
AGAAAATTACAATTGCAAAA+AGG 0.275424 3_4:+77268431 None:intergenic
TCATAAGACCTTCATGTATT+AGG 0.301699 3_4:-77271135 Msa0523820:five_prime_UTR
TCACAAAAGTTGTTGAAAAT+TGG 0.305355 3_4:-77268781 Msa0523820:three_prime_UTR
CTCATTCACTCTTCAATTGC+TGG 0.307759 3_4:-77270618 Msa0523820:CDS
AATTGAAGAGTGAATGAGTT+TGG 0.318103 3_4:+77270623 None:intergenic
CATAAAGTGGATTACAACTT+AGG 0.332434 3_4:-77268350 Msa0523820:three_prime_UTR
TGACCATTCATTGGATTAAT+CGG 0.332530 3_4:-77267966 Msa0523820:three_prime_UTR
GCTTCTTGTTGAATTGAAAC+AGG 0.337554 3_4:+77271023 None:intergenic
GATCAAAATTGAAGTTGTAT+AGG 0.339897 3_4:+77269273 None:intergenic
TTCACAAGGCTTACGCTTAT+GGG 0.341692 3_4:-77268259 Msa0523820:three_prime_UTR
ATTGGGATTCCCGGTGATCT+TGG 0.342453 3_4:+77270351 None:intergenic
ATTGAAGAGTGAATGAGTTT+GGG 0.357408 3_4:+77270624 None:intergenic
CCAATAAGAATGGAGGAAAA+AGG 0.365761 3_4:+77268873 None:intergenic
TTGAATACTACGACTAGTTC+CGG 0.373329 3_4:-77270921 Msa0523820:CDS
GAAAATCTGTGACCATTCAT+TGG 0.376630 3_4:-77267975 Msa0523820:three_prime_UTR
GATAGAAAAGAAAGAGAGAA+AGG 0.378705 3_4:-77268218 Msa0523820:three_prime_UTR
GAATCTTGATAAGAGGCATT+TGG 0.383503 3_4:+77267933 None:intergenic
ATGTCACATGGTTATCTTAG+AGG 0.386262 3_4:+77268742 None:intergenic
CCAGGATGCAACAAAATTAC+AGG 0.386637 3_4:-77270034 Msa0523820:CDS
TTTCAGCTTAGTATAGTAGA+TGG 0.388434 3_4:+77271062 None:intergenic
AAAGACAAGAGGATCAAAAT+AGG 0.388939 3_4:-77270391 Msa0523820:CDS
TATGTATGATGCAGGTTAGA+AGG 0.402202 3_4:-77270099 Msa0523820:intron
TTTATGCTTGATCAAAATGA+AGG 0.406384 3_4:+77269090 None:intergenic
TATACAGACAATTAATATAT+AGG 0.408939 3_4:+77267888 None:intergenic
TTGCTTGTTGTTTAGGCTGT+TGG 0.409201 3_4:-77270427 Msa0523820:intron
AGTGATATGATTTGAAAGAT+AGG 0.424567 3_4:-77268171 Msa0523820:three_prime_UTR
AGCTATGGCTGTCGGTGGCT+TGG 0.431984 3_4:+77270209 None:intergenic
GCGTGTTCGACGACACATAT+CGG 0.432380 3_4:+77268631 None:intergenic
ACATATACTATGCTAGGAAT+AGG 0.436983 3_4:-77268194 Msa0523820:three_prime_UTR
ATAAAGTGGATTACAACTTA+GGG 0.437155 3_4:-77268349 Msa0523820:three_prime_UTR
ATTCAATTCAATTATGCAGC+TGG 0.439547 3_4:-77269001 Msa0523820:three_prime_UTR
ACTCTCAGTTGCTAAATTCC+CGG 0.447140 3_4:+77270902 None:intergenic
TTTAAAGGTTTATAGCTTTG+AGG 0.448592 3_4:-77268397 Msa0523820:three_prime_UTR
CACGCACACACCTTGAGCTT+AGG 0.457558 3_4:+77270540 None:intergenic
TGCTCGAACATGAATGTAAT+CGG 0.461397 3_4:+77270240 None:intergenic
TGTAAGTGTTTGTATTGTAT+CGG 0.473692 3_4:-77268657 Msa0523820:three_prime_UTR
AAAATGATTGACGCTATTGC+TGG 0.474007 3_4:+77268373 None:intergenic
AGAGTTGGCATTTGAAGAGT+TGG 0.474811 3_4:+77270705 None:intergenic
TGAATACTACGACTAGTTCC+GGG 0.477955 3_4:-77270920 Msa0523820:CDS
ATAGAAAAGAAAGAGAGAAA+GGG 0.478037 3_4:-77268217 Msa0523820:three_prime_UTR
TTCTCAAATTATTACAGTGT+CGG 0.481525 3_4:+77268701 None:intergenic
AACAAAATTACAGGAAAAGC+TGG 0.485342 3_4:-77270025 Msa0523820:CDS
GAGTGAATGAGTTTGGGGTC+TGG 0.491043 3_4:+77270630 None:intergenic
ATGTATGATGCAGGTTAGAA+GGG 0.501649 3_4:-77270098 Msa0523820:intron
AATATTTCCATATTTAGTGT+TGG 0.505714 3_4:+77268097 None:intergenic
TAAAATGGGAGATTGTGAAA+TGG 0.508049 3_4:+77268313 None:intergenic
TTGTCATTGTCCAATAAGAA+TGG 0.509664 3_4:+77268863 None:intergenic
AAGCCGATTAATCCAATGAA+TGG 0.510551 3_4:+77267963 None:intergenic
TGTGAAATAAATTGGATAGG+AGG 0.511950 3_4:-77268468 Msa0523820:three_prime_UTR
TCATTCACTCTTCAATTGCT+GGG 0.512372 3_4:-77270617 Msa0523820:CDS
TTATGCTTGATCAAAATGAA+GGG 0.512781 3_4:+77269091 None:intergenic
TCAATATTGAAATCAAGCCT+TGG 0.519925 3_4:+77269805 None:intergenic
GAAGTAGTAACATCAAGAGT+TGG 0.521259 3_4:+77270690 None:intergenic
AACAGTGTTTGAAAGACAAA+GGG 0.521570 3_4:-77270865 Msa0523820:CDS
ATTACATAGCATTAAAGTAG+TGG 0.524886 3_4:+77268952 None:intergenic
GCATTAAAGTAGTGGGAAAG+TGG 0.525136 3_4:+77268960 None:intergenic
TTACATAGCATTAAAGTAGT+GGG 0.530181 3_4:+77268953 None:intergenic
TTACTCTTTCAGCTAAGCTA+TGG 0.530789 3_4:+77270194 None:intergenic
TGAGTGGGTTTAATAGATGA+TGG 0.532006 3_4:-77269126 Msa0523820:three_prime_UTR
AAAGGGACATATACTATGCT+AGG 0.535698 3_4:-77268200 Msa0523820:three_prime_UTR
GGAAAGGAAAGCAAAGCAGT+AGG 0.537257 3_4:+77271103 None:intergenic
AAGTAGAGGCAAAAGTGAGT+GGG 0.540822 3_4:-77269141 Msa0523820:three_prime_UTR
GGAAGAATACTTGAGAGGAA+AGG 0.544208 3_4:+77271087 None:intergenic
CTTCTTGTTGAATTGAAACA+GGG 0.549462 3_4:+77271024 None:intergenic
AAAGTAGAGGCAAAAGTGAG+TGG 0.552885 3_4:-77269142 Msa0523820:three_prime_UTR
AGAAGATACTTCAAACTCAA+AGG 0.553682 3_4:-77270293 Msa0523820:CDS
GTGTTGTTTGACATGGATGC+AGG 0.554052 3_4:-77268039 Msa0523820:three_prime_UTR
TCTAAAAGTGTTGTTTGACA+TGG 0.555725 3_4:-77268046 Msa0523820:three_prime_UTR
TTCAGCTAAGCTATGGCTGT+CGG 0.556309 3_4:+77270201 None:intergenic
TAACAGTGTTTGAAAGACAA+AGG 0.558772 3_4:-77270866 Msa0523820:CDS
TAATACATGAAGGTCTTATG+AGG 0.559647 3_4:+77271137 None:intergenic
ACTAATACTTTGTTCAGCTT+AGG 0.562469 3_4:-77268572 Msa0523820:three_prime_UTR
TATCTGCAAGATTTAGTACC+AGG 0.562856 3_4:-77270052 Msa0523820:CDS
AGCTTAGTATAGTAGATGGA+AGG 0.566505 3_4:+77271066 None:intergenic
GACGGCGAATCCAAGATCAC+CGG 0.574848 3_4:-77270361 Msa0523820:CDS
AGTTTGGGGTCTGGAAGAAG+AGG 0.575334 3_4:+77270639 None:intergenic
TCATCATAATCCTAAGCTCA+AGG 0.584084 3_4:-77270550 Msa0523820:intron
GTAAATAACTGAGATTGAGA+AGG 0.584711 3_4:+77269230 None:intergenic
TAAATCAGCAGCAAGTGAAG+AGG 0.586756 3_4:+77270731 None:intergenic
TTCAAACACTGTTATGTCAG+AGG 0.587410 3_4:+77270875 None:intergenic
TGGAAGGAAGAATACTTGAG+AGG 0.590986 3_4:+77271082 None:intergenic
TTGGGGTCTGGAAGAAGAGG+TGG 0.593623 3_4:+77270642 None:intergenic
CTACCTAATACAACATGGGA+AGG 0.593979 3_4:-77269308 Msa0523820:CDS
GAAAGGAAAGCAAAGCAGTA+GGG 0.594538 3_4:+77271104 None:intergenic
TATGCTTGATCAAAATGAAG+GGG 0.598468 3_4:+77269092 None:intergenic
AGCTAAGCTATGGCTGTCGG+TGG 0.605688 3_4:+77270204 None:intergenic
AATTAGCTACTGTTAATCCA+AGG 0.613412 3_4:-77269822 Msa0523820:CDS
GGAGGAATAGAGTGAAACGT+AGG 0.621607 3_4:+77271158 None:intergenic
AAATTCTACCTAATACAACA+TGG 0.624191 3_4:-77269313 Msa0523820:CDS
TACATGAAGGTCTTATGAGG+AGG 0.627936 3_4:+77271140 None:intergenic
ACGGCGAATCCAAGATCACC+GGG 0.628691 3_4:-77270360 Msa0523820:CDS
ATCTTTAAAGTAGAAAGTAG+AGG 0.628972 3_4:-77269155 Msa0523820:three_prime_UTR
ATCAAAATAGGTGCTGAGGA+CGG 0.629527 3_4:-77270379 Msa0523820:CDS
TTGAAGAGTGAATGAGTTTG+GGG 0.630480 3_4:+77270625 None:intergenic
TCAATGCACCTAATACATGA+AGG 0.633342 3_4:+77271127 None:intergenic
GACAAAGGGAAAGAATGAAG+TGG 0.633675 3_4:-77270851 Msa0523820:CDS
GAGGATCAAAATAGGTGCTG+AGG 0.635802 3_4:-77270383 Msa0523820:CDS
AAGTTGAGAACAAAGACAAG+AGG 0.638388 3_4:-77270402 Msa0523820:CDS
ATTCATGTTCGAGCACGTCG+TGG 0.642577 3_4:-77270232 Msa0523820:CDS
TTGAGCTTAGGATTATGATG+AGG 0.647091 3_4:+77270552 None:intergenic
TCATTGTCCAATAAGAATGG+AGG 0.648557 3_4:+77268866 None:intergenic
AATTCTACCTAATACAACAT+GGG 0.654432 3_4:-77269312 Msa0523820:CDS
GAGAAAATGATAGTTTCACA+AGG 0.676686 3_4:-77268273 Msa0523820:three_prime_UTR
TTCTTGTTGAATTGAAACAG+GGG 0.686215 3_4:+77271025 None:intergenic
TGCTATGTAATAAGATAACG+AGG 0.691479 3_4:-77268940 Msa0523820:three_prime_UTR
TGTCATTGTATGATGTCACA+TGG 0.727583 3_4:+77268730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAGTGAATTAAT+TGG - chr3_4:77268525-77268544 Msa0523820:three_prime_UTR 10.0%
!!! ACTAAAGATTTTTTTTTAAA+TGG - chr3_4:77269972-77269991 Msa0523820:intron 10.0%
!! TTCACAAAATATAATTAATT+TGG + chr3_4:77270526-77270545 None:intergenic 10.0%
!!! TTATATTTTGTGAAATAAAT+TGG - chr3_4:77270532-77270551 Msa0523820:intron 10.0%
!! TAAGTAGTAATAATATACTT+TGG - chr3_4:77268834-77268853 Msa0523820:three_prime_UTR 15.0%
!!! TAACATAAACAAATTTGTTA+TGG + chr3_4:77269140-77269159 None:intergenic 15.0%
!! TTGAGAAAATAAAAAAGATT+TGG - chr3_4:77270323-77270342 Msa0523820:CDS 15.0%
!! TGAGAAAATAAAAAAGATTT+GGG - chr3_4:77270324-77270343 Msa0523820:CDS 15.0%
!! TATACAGACAATTAATATAT+AGG + chr3_4:77271123-77271142 None:intergenic 15.0%
!! TAATTATATATGTATGATGC+AGG - chr3_4:77268901-77268920 Msa0523820:three_prime_UTR 20.0%
!!! ATAAACAAATTTGTTATGGA+TGG + chr3_4:77269136-77269155 None:intergenic 20.0%
!! ATTAAGCTAAAATACAGATT+AGG + chr3_4:77269287-77269306 None:intergenic 20.0%
!! AAAATTAAAACACTAGTAAC+GGG + chr3_4:77269624-77269643 None:intergenic 20.0%
!! CAAAATTAAAACACTAGTAA+CGG + chr3_4:77269625-77269644 None:intergenic 20.0%
!!! GTGTTTTAATTTTGTTGTAT+TGG - chr3_4:77269631-77269650 Msa0523820:intron 20.0%
!! GTTCATTATTGAAAAACAAA+AGG + chr3_4:77269954-77269973 None:intergenic 20.0%
!!! CAATTTTCAACAACTTTTTA+GGG - chr3_4:77270108-77270127 Msa0523820:intron 20.0%
!!! CAATGACATTTTAGAATTAA+AGG + chr3_4:77270402-77270421 None:intergenic 20.0%
!!! AAAAAAATGCATAAGTTGAA+TGG - chr3_4:77270475-77270494 Msa0523820:intron 20.0%
!!! TTTTGTGAAATAAATTGGAT+AGG - chr3_4:77270537-77270556 Msa0523820:intron 20.0%
!! GAAAATTACAATTGCAAAAA+GGG + chr3_4:77270579-77270598 None:intergenic 20.0%
!! AGAAAATTACAATTGCAAAA+AGG + chr3_4:77270580-77270599 None:intergenic 20.0%
!! AATATTTCCATATTTAGTGT+TGG + chr3_4:77270914-77270933 None:intergenic 20.0%
!!! TAATTTGTTGCTTGTTGTTT+AGG - chr3_4:77268574-77268593 Msa0523820:three_prime_UTR 25.0%
! TGATGATCTATTTGAAAAAG+AGG - chr3_4:77269223-77269242 Msa0523820:CDS 25.0%
! ATCAATAAAAGTTTGAGGAA+TGG + chr3_4:77269555-77269574 None:intergenic 25.0%
! GAAGAATCAATAAAAGTTTG+AGG + chr3_4:77269560-77269579 None:intergenic 25.0%
! AAAAATATTAACAGAGAGAG+TGG + chr3_4:77269599-77269618 None:intergenic 25.0%
! AAATTCTACCTAATACAACA+TGG - chr3_4:77269695-77269714 Msa0523820:intron 25.0%
! AATTCTACCTAATACAACAT+GGG - chr3_4:77269696-77269715 Msa0523820:intron 25.0%
!! AATTGAAGTTGTATAGGTTT+TGG + chr3_4:77269732-77269751 None:intergenic 25.0%
! GATCAAAATTGAAGTTGTAT+AGG + chr3_4:77269738-77269757 None:intergenic 25.0%
!!! ATCTTTAAAGTAGAAAGTAG+AGG - chr3_4:77269853-77269872 Msa0523820:intron 25.0%
! TTATGCTTGATCAAAATGAA+GGG + chr3_4:77269920-77269939 None:intergenic 25.0%
! TTTATGCTTGATCAAAATGA+AGG + chr3_4:77269921-77269940 None:intergenic 25.0%
!! TATTGAAAAACAAAAGGTAC+TGG + chr3_4:77269948-77269967 None:intergenic 25.0%
! TTACATAGCATTAAAGTAGT+GGG + chr3_4:77270058-77270077 None:intergenic 25.0%
! ATTACATAGCATTAAAGTAG+TGG + chr3_4:77270059-77270078 None:intergenic 25.0%
! CCTAAAAAGTTGTTGAAAAT+TGG + chr3_4:77270110-77270129 None:intergenic 25.0%
!! CCAATTTTCAACAACTTTTT+AGG - chr3_4:77270107-77270126 Msa0523820:intron 25.0%
! TCACAAAAGTTGTTGAAAAT+TGG - chr3_4:77270227-77270246 Msa0523820:CDS 25.0%
! TTCTCAAATTATTACAGTGT+CGG + chr3_4:77270310-77270329 None:intergenic 25.0%
! TGTAAGTGTTTGTATTGTAT+CGG - chr3_4:77270351-77270370 Msa0523820:CDS 25.0%
!! ATTGTAATTTTCTTGCAACT+TGG - chr3_4:77270587-77270606 Msa0523820:CDS 25.0%
!! TTTAAAGGTTTATAGCTTTG+AGG - chr3_4:77270611-77270630 Msa0523820:CDS 25.0%
!!! ATAAAGTGGATTACAACTTA+GGG - chr3_4:77270659-77270678 Msa0523820:CDS 25.0%
!!! TTTTAAGTGGTCTCTAAAAT+GGG + chr3_4:77270712-77270731 None:intergenic 25.0%
!!! ATTTTAAGTGGTCTCTAAAA+TGG + chr3_4:77270713-77270732 None:intergenic 25.0%
!! TCATTTTCTCTGATTTTAAG+TGG + chr3_4:77270725-77270744 None:intergenic 25.0%
! ATAGAAAAGAAAGAGAGAAA+GGG - chr3_4:77270791-77270810 Msa0523820:CDS 25.0%
! AGTGATATGATTTGAAAGAT+AGG - chr3_4:77270837-77270856 Msa0523820:CDS 25.0%
! GAAAAAAGAATCTTGATAAG+AGG + chr3_4:77271085-77271104 None:intergenic 25.0%
! TAATACATGAAGGTCTTATG+AGG + chr3_4:77267874-77267893 None:intergenic 30.0%
TCATAAGACCTTCATGTATT+AGG - chr3_4:77267873-77267892 Msa0523820:three_prime_UTR 30.0%
TTTCAGCTTAGTATAGTAGA+TGG + chr3_4:77267949-77267968 None:intergenic 30.0%
TTCTTGTTGAATTGAAACAG+GGG + chr3_4:77267986-77268005 None:intergenic 30.0%
CTTCTTGTTGAATTGAAACA+GGG + chr3_4:77267987-77268006 None:intergenic 30.0%
TAACAGTGTTTGAAAGACAA+AGG - chr3_4:77268142-77268161 Msa0523820:three_prime_UTR 30.0%
AACAGTGTTTGAAAGACAAA+GGG - chr3_4:77268143-77268162 Msa0523820:three_prime_UTR 30.0%
ATTGAAGAGTGAATGAGTTT+GGG + chr3_4:77268387-77268406 None:intergenic 30.0%
AATTGAAGAGTGAATGAGTT+TGG + chr3_4:77268388-77268407 None:intergenic 30.0%
AAAGACAAGAGGATCAAAAT+AGG - chr3_4:77268617-77268636 Msa0523820:three_prime_UTR 30.0%
!!! TTGGTGTTGTTTTTGGTATT+GGG + chr3_4:77268677-77268696 None:intergenic 30.0%
!!! TTCTTTGTTGGTGTTGTTTT+TGG + chr3_4:77268684-77268703 None:intergenic 30.0%
AGAAGATACTTCAAACTCAA+AGG - chr3_4:77268715-77268734 Msa0523820:three_prime_UTR 30.0%
AACAAAATTACAGGAAAAGC+TGG - chr3_4:77268983-77269002 Msa0523820:three_prime_UTR 30.0%
CATAGATAGATCAATGTGAT+AGG + chr3_4:77269111-77269130 None:intergenic 30.0%
AATTAGCTACTGTTAATCCA+AGG - chr3_4:77269186-77269205 Msa0523820:three_prime_UTR 30.0%
TCAATATTGAAATCAAGCCT+TGG + chr3_4:77269206-77269225 None:intergenic 30.0%
GGTAATAAAGAATATGCAGA+TGG - chr3_4:77269244-77269263 Msa0523820:CDS 30.0%
!! TTTGTGTTGATTCCTTACAT+AGG - chr3_4:77269349-77269368 Msa0523820:intron 30.0%
AAATGCAAACTTTCAAGCTA+TGG - chr3_4:77269391-77269410 Msa0523820:intron 30.0%
AATGCAAACTTTCAAGCTAT+GGG - chr3_4:77269392-77269411 Msa0523820:intron 30.0%
AAGTTTGAGGAATGGATATA+GGG + chr3_4:77269547-77269566 None:intergenic 30.0%
AAAGTTTGAGGAATGGATAT+AGG + chr3_4:77269548-77269567 None:intergenic 30.0%
GTAAATAACTGAGATTGAGA+AGG + chr3_4:77269781-77269800 None:intergenic 30.0%
TCTCAATCTCAGTTATTTAC+AGG - chr3_4:77269781-77269800 Msa0523820:intron 30.0%
TATGCTTGATCAAAATGAAG+GGG + chr3_4:77269919-77269938 None:intergenic 30.0%
ATTCAATTCAATTATGCAGC+TGG - chr3_4:77270007-77270026 Msa0523820:CDS 30.0%
TGCTATGTAATAAGATAACG+AGG - chr3_4:77270068-77270087 Msa0523820:CDS 30.0%
TTGTCATTGTCCAATAAGAA+TGG + chr3_4:77270148-77270167 None:intergenic 30.0%
ACTAATACTTTGTTCAGCTT+AGG - chr3_4:77270436-77270455 Msa0523820:intron 30.0%
TGTGAAATAAATTGGATAGG+AGG - chr3_4:77270540-77270559 Msa0523820:intron 30.0%
TTCTTGCAACTTGGATTTAA+AGG - chr3_4:77270596-77270615 Msa0523820:CDS 30.0%
! CGTCAATCATTTTCATAAAG+TGG - chr3_4:77270645-77270664 Msa0523820:CDS 30.0%
!! CATAAAGTGGATTACAACTT+AGG - chr3_4:77270658-77270677 Msa0523820:CDS 30.0%
TAAAATGGGAGATTGTGAAA+TGG + chr3_4:77270698-77270717 None:intergenic 30.0%
GAGAAAATGATAGTTTCACA+AGG - chr3_4:77270735-77270754 Msa0523820:CDS 30.0%
GATAGAAAAGAAAGAGAGAA+AGG - chr3_4:77270790-77270809 Msa0523820:CDS 30.0%
ACATATACTATGCTAGGAAT+AGG - chr3_4:77270814-77270833 Msa0523820:CDS 30.0%
GAATGTTCCAACACTAAATA+TGG - chr3_4:77270904-77270923 Msa0523820:CDS 30.0%
!! TCTAAAAGTGTTGTTTGACA+TGG - chr3_4:77270962-77270981 Msa0523820:five_prime_UTR 30.0%
TGACCATTCATTGGATTAAT+CGG - chr3_4:77271042-77271061 Msa0523820:five_prime_UTR 30.0%
TCAATGCACCTAATACATGA+AGG + chr3_4:77267884-77267903 None:intergenic 35.0%
AGCTTAGTATAGTAGATGGA+AGG + chr3_4:77267945-77267964 None:intergenic 35.0%
GCTTCTTGTTGAATTGAAAC+AGG + chr3_4:77267988-77268007 None:intergenic 35.0%
! TTGAATACTACGACTAGTTC+CGG - chr3_4:77268087-77268106 Msa0523820:three_prime_UTR 35.0%
TTCAAACACTGTTATGTCAG+AGG + chr3_4:77268136-77268155 None:intergenic 35.0%
CAACAACAACAACAACAACA+AGG - chr3_4:77268180-77268199 Msa0523820:three_prime_UTR 35.0%
GAAGTAGTAACATCAAGAGT+TGG + chr3_4:77268321-77268340 None:intergenic 35.0%
TTGAAGAGTGAATGAGTTTG+GGG + chr3_4:77268386-77268405 None:intergenic 35.0%
TCATTCACTCTTCAATTGCT+GGG - chr3_4:77268391-77268410 Msa0523820:three_prime_UTR 35.0%
! GGATTATGATGAGGTTTTTC+AGG + chr3_4:77268450-77268469 None:intergenic 35.0%
TTGAGCTTAGGATTATGATG+AGG + chr3_4:77268459-77268478 None:intergenic 35.0%
TCATCATAATCCTAAGCTCA+AGG - chr3_4:77268458-77268477 Msa0523820:three_prime_UTR 35.0%
AAGTTGAGAACAAAGACAAG+AGG - chr3_4:77268606-77268625 Msa0523820:three_prime_UTR 35.0%
!!! GTTGGTGTTGTTTTTGGTAT+TGG + chr3_4:77268678-77268697 None:intergenic 35.0%
! TGCTACACAGTTTTCTTTGT+TGG + chr3_4:77268696-77268715 None:intergenic 35.0%
TGCTCGAACATGAATGTAAT+CGG + chr3_4:77268771-77268790 None:intergenic 35.0%
TTACTCTTTCAGCTAAGCTA+TGG + chr3_4:77268817-77268836 None:intergenic 35.0%
TATGTATGATGCAGGTTAGA+AGG - chr3_4:77268909-77268928 Msa0523820:three_prime_UTR 35.0%
ATGTATGATGCAGGTTAGAA+GGG - chr3_4:77268910-77268929 Msa0523820:three_prime_UTR 35.0%
TATCTGCAAGATTTAGTACC+AGG - chr3_4:77268956-77268975 Msa0523820:three_prime_UTR 35.0%
GTCTCTTCAAAAACAAGTTG+AGG - chr3_4:77269036-77269055 Msa0523820:three_prime_UTR 35.0%
AAGTAGGAAACACCTATGTA+AGG + chr3_4:77269364-77269383 None:intergenic 35.0%
TTTGCATTTGCATCACAAGT+AGG + chr3_4:77269380-77269399 None:intergenic 35.0%
GGTGAATTGAACTGAAGATA+AGG + chr3_4:77269446-77269465 None:intergenic 35.0%
TTCTAAGTCCCATATCAGTA+GGG + chr3_4:77269511-77269530 None:intergenic 35.0%
AGTTTGAGGAATGGATATAG+GGG + chr3_4:77269546-77269565 None:intergenic 35.0%
! GATTCTTCTTGTTTCTCTGT+AGG - chr3_4:77269572-77269591 Msa0523820:intron 35.0%
! TGAGTGGGTTTAATAGATGA+TGG - chr3_4:77269882-77269901 Msa0523820:intron 35.0%
CCAATAAGAATGGAGGAAAA+AGG + chr3_4:77270138-77270157 None:intergenic 35.0%
! CCTTTTTCCTCCATTCTTAT+TGG - chr3_4:77270135-77270154 Msa0523820:intron 35.0%
TCATTGTCCAATAAGAATGG+AGG + chr3_4:77270145-77270164 None:intergenic 35.0%
ATGTCACATGGTTATCTTAG+AGG + chr3_4:77270269-77270288 None:intergenic 35.0%
TGTCATTGTATGATGTCACA+TGG + chr3_4:77270281-77270300 None:intergenic 35.0%
AAAATGATTGACGCTATTGC+TGG + chr3_4:77270638-77270657 None:intergenic 35.0%
! TTACAACTTAGGGTTGTTTC+TGG - chr3_4:77270669-77270688 Msa0523820:CDS 35.0%
AAAGGGACATATACTATGCT+AGG - chr3_4:77270808-77270827 Msa0523820:CDS 35.0%
! ATGAATGGTCACAGATTTTC+TGG + chr3_4:77271033-77271052 None:intergenic 35.0%
GAAAATCTGTGACCATTCAT+TGG - chr3_4:77271033-77271052 Msa0523820:five_prime_UTR 35.0%
AAGCCGATTAATCCAATGAA+TGG + chr3_4:77271048-77271067 None:intergenic 35.0%
!! GAATCTTGATAAGAGGCATT+TGG + chr3_4:77271078-77271097 None:intergenic 35.0%
! TACATGAAGGTCTTATGAGG+AGG + chr3_4:77267871-77267890 None:intergenic 40.0%
GAAAGGAAAGCAAAGCAGTA+GGG + chr3_4:77267907-77267926 None:intergenic 40.0%
GGAAGAATACTTGAGAGGAA+AGG + chr3_4:77267924-77267943 None:intergenic 40.0%
TGGAAGGAAGAATACTTGAG+AGG + chr3_4:77267929-77267948 None:intergenic 40.0%
TGTGACAGTGTTAGTGTCTT+TGG + chr3_4:77268010-77268029 None:intergenic 40.0%
! TGAATACTACGACTAGTTCC+GGG - chr3_4:77268088-77268107 Msa0523820:three_prime_UTR 40.0%
ACTCTCAGTTGCTAAATTCC+CGG + chr3_4:77268109-77268128 None:intergenic 40.0%
GACAAAGGGAAAGAATGAAG+TGG - chr3_4:77268157-77268176 Msa0523820:three_prime_UTR 40.0%
TAAATCAGCAGCAAGTGAAG+AGG + chr3_4:77268280-77268299 None:intergenic 40.0%
AGAGTTGGCATTTGAAGAGT+TGG + chr3_4:77268306-77268325 None:intergenic 40.0%
CTCATTCACTCTTCAATTGC+TGG - chr3_4:77268390-77268409 Msa0523820:three_prime_UTR 40.0%
!! TTGCTTGTTGTTTAGGCTGT+TGG - chr3_4:77268581-77268600 Msa0523820:three_prime_UTR 40.0%
!! ATCAAAATAGGTGCTGAGGA+CGG - chr3_4:77268629-77268648 Msa0523820:three_prime_UTR 40.0%
!!! GTTTTTGGTATTGGGATTCC+CGG + chr3_4:77268669-77268688 None:intergenic 40.0%
! CCTGTAATTTTGTTGCATCC+TGG + chr3_4:77268977-77268996 None:intergenic 40.0%
CCAGGATGCAACAAAATTAC+AGG - chr3_4:77268974-77268993 Msa0523820:three_prime_UTR 40.0%
CCATAAGGAACAAATTGCTG+TGG + chr3_4:77269467-77269486 None:intergenic 40.0%
! CCACAGCAATTTGTTCCTTA+TGG - chr3_4:77269464-77269483 Msa0523820:intron 40.0%
! CAGCAATTTGTTCCTTATGG+TGG - chr3_4:77269467-77269486 Msa0523820:intron 40.0%
TGGAATGAACCCTACTGATA+TGG - chr3_4:77269499-77269518 Msa0523820:intron 40.0%
GGAATGAACCCTACTGATAT+GGG - chr3_4:77269500-77269519 Msa0523820:intron 40.0%
CTTCTAAGTCCCATATCAGT+AGG + chr3_4:77269512-77269531 None:intergenic 40.0%
CTACTGATATGGGACTTAGA+AGG - chr3_4:77269510-77269529 Msa0523820:intron 40.0%
CTACCTAATACAACATGGGA+AGG - chr3_4:77269700-77269719 Msa0523820:intron 40.0%
TCACCTTCCCATGTTGTATT+AGG + chr3_4:77269706-77269725 None:intergenic 40.0%
AAAGTAGAGGCAAAAGTGAG+TGG - chr3_4:77269866-77269885 Msa0523820:intron 40.0%
AAGTAGAGGCAAAAGTGAGT+GGG - chr3_4:77269867-77269886 Msa0523820:intron 40.0%
! GCATTAAAGTAGTGGGAAAG+TGG + chr3_4:77270051-77270070 None:intergenic 40.0%
TTTCACAAGGCTTACGCTTA+TGG - chr3_4:77270748-77270767 Msa0523820:CDS 40.0%
TTCACAAGGCTTACGCTTAT+GGG - chr3_4:77270749-77270768 Msa0523820:CDS 40.0%
GGAAAGGAAAGCAAAGCAGT+AGG + chr3_4:77267908-77267927 None:intergenic 45.0%
! GAGGATCAAAATAGGTGCTG+AGG - chr3_4:77268625-77268644 Msa0523820:three_prime_UTR 45.0%
TTCAGCTAAGCTATGGCTGT+CGG + chr3_4:77268810-77268829 None:intergenic 45.0%
CCAGCATCTAATCCACCATA+AGG + chr3_4:77269482-77269501 None:intergenic 45.0%
!! CCTTATGGTGGATTAGATGC+TGG - chr3_4:77269479-77269498 Msa0523820:intron 45.0%
!! GTGTTGTTTGACATGGATGC+AGG - chr3_4:77270969-77270988 Msa0523820:five_prime_UTR 45.0%
!!! ATATGATTTTATTTATATTA+AGG + chr3_4:77271151-77271170 None:intergenic 5.0%
! AGTTTGGGGTCTGGAAGAAG+AGG + chr3_4:77268372-77268391 None:intergenic 50.0%
! GAGTGAATGAGTTTGGGGTC+TGG + chr3_4:77268381-77268400 None:intergenic 50.0%
ATTGGGATTCCCGGTGATCT+TGG + chr3_4:77268660-77268679 None:intergenic 50.0%
ATTCATGTTCGAGCACGTCG+TGG - chr3_4:77268776-77268795 Msa0523820:three_prime_UTR 50.0%
GCGTGTTCGACGACACATAT+CGG + chr3_4:77270380-77270399 None:intergenic 50.0%
TTGGGGTCTGGAAGAAGAGG+TGG + chr3_4:77268369-77268388 None:intergenic 55.0%
CACGCACACACCTTGAGCTT+AGG + chr3_4:77268471-77268490 None:intergenic 55.0%
GACGGCGAATCCAAGATCAC+CGG - chr3_4:77268647-77268666 Msa0523820:three_prime_UTR 55.0%
ACGGCGAATCCAAGATCACC+GGG - chr3_4:77268648-77268667 Msa0523820:three_prime_UTR 55.0%
AGCTAAGCTATGGCTGTCGG+TGG + chr3_4:77268807-77268826 None:intergenic 55.0%
AGCTATGGCTGTCGGTGGCT+TGG + chr3_4:77268802-77268821 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 77267851 77271179 77267851 ID=Msa0523820;Name=Msa0523820
chr3_4 mRNA 77267851 77271179 77267851 ID=Msa0523820-mRNA-1;Parent=Msa0523820;Name=Msa0523820-mRNA-1;_AED=0.08;_eAED=0.08;_QI=224|0.75|1|1|1|1|5|1369|322
chr3_4 exon 77267851 77269339 77267851 ID=Msa0523820-mRNA-1:exon:22592;Parent=Msa0523820-mRNA-1
chr3_4 exon 77269786 77269857 77269786 ID=Msa0523820-mRNA-1:exon:22591;Parent=Msa0523820-mRNA-1
chr3_4 exon 77269973 77270107 77269973 ID=Msa0523820-mRNA-1:exon:22590;Parent=Msa0523820-mRNA-1
chr3_4 exon 77270198 77270434 77270198 ID=Msa0523820-mRNA-1:exon:22589;Parent=Msa0523820-mRNA-1
chr3_4 exon 77270551 77271179 77270551 ID=Msa0523820-mRNA-1:exon:22588;Parent=Msa0523820-mRNA-1
chr3_4 five_prime_UTR 77270956 77271179 77270956 ID=Msa0523820-mRNA-1:five_prime_utr;Parent=Msa0523820-mRNA-1
chr3_4 CDS 77270551 77270955 77270551 ID=Msa0523820-mRNA-1:cds;Parent=Msa0523820-mRNA-1
chr3_4 CDS 77270198 77270434 77270198 ID=Msa0523820-mRNA-1:cds;Parent=Msa0523820-mRNA-1
chr3_4 CDS 77269973 77270107 77269973 ID=Msa0523820-mRNA-1:cds;Parent=Msa0523820-mRNA-1
chr3_4 CDS 77269786 77269857 77269786 ID=Msa0523820-mRNA-1:cds;Parent=Msa0523820-mRNA-1
chr3_4 CDS 77269220 77269339 77269220 ID=Msa0523820-mRNA-1:cds;Parent=Msa0523820-mRNA-1
chr3_4 three_prime_UTR 77267851 77269219 77267851 ID=Msa0523820-mRNA-1:three_prime_utr;Parent=Msa0523820-mRNA-1
Gene Sequence

>Msa0523820

ATGTTGCACTGTTTGAATACTACGACTAGTTCCGGGAATTTAGCAACTGAGAGTTGCTCCTCTGACATAACAGTGTTTGAAAGACAAAGGGAAAGAATGAAGTGGCAACAACAACAACAACAACAAGGTTACTTCAACGCTGCTGCTATGTTCTGTTCTTCTCTGCAACAACAACAACAACAACAAGTTGACAATTCTGAAGCCTCTTCACTTGCTGCTGATTTACTGCCAACTCTTCAAATGCCAACTCTTGATGTTACTACTTCTGCTATTTCAAGAACTTTCAGCTGTCCACCTCTTCTTCCAGACCCCAAACTCATTCACTCTTCAATTGCTGGGAAAGACAAACACAATTCTTCTAAGAAAAGAAAACCTGAAAAACCTCATCATAATCCTAAGCTCAAGGCTGTTGGTGAAGTTGAGAACAAAGACAAGAGGATCAAAATAGGTGCTGAGGACGGCGAATCCAAGATCACCGGGAATCCCAATACCAAAAACAACACCAACAAAGAAAACTGTGTAGCAGAAGATACTTCAAACTCAAAGGAAAATTCAAAAGTTTCTGATGTTCAAAAGACCGATTACATTCATGTTCGAGCACGTCGTGGCCAAGCCACCGACAGCCATAGCTTAGCTGAAAGAGTTAGAAGGGAAAAGATTAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGATGCAACAAAATTACAGGAAAAGCTGGAATGCTTGATGAAATCATCAACTATGTTCAGTCTCTTCAAAAACAAGTTGAGTTCTTGTCAATGAAATTAGCTACTGTTAATCCAAGGCTTGATTTCAATATTGATGATCTATTTGAAAAAGAGCAGCAAATTCTACCTAATACAACATGGGAAGGTGATTTCCAAAACCTATACAACTTCAATTTTGATCAAGCAAGAGCAACATCTTTTCCTTCTCAATCTCAGTTATTTACAGGTAGTTAA
Protein sequence

>Msa0523820

MLHCLNTTTSSGNLATESCSSDITVFERQRERMKWQQQQQQQGYFNAAAMFCSSLQQQQQQQVDNSEASSLAADLLPTLQMPTLDVTTSAISRTFSCPPLLPDPKLIHSSIAGKDKHNSSKKRKPEKPHHNPKLKAVGEVENKDKRIKIGAEDGESKITGNPNTKNNTNKENCVAEDTSNSKENSKVSDVQKTDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQKQVEFLSMKLATVNPRLDFNIDDLFEKEQQILPNTTWEGDFQNLYNFNFDQARATSFPSQSQLFTGS*