AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0528040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0528040 MtrunA17_Chr2g0324781 96.163 417 7 5 1 410 1 415 0.0 815
Msa0528040 MtrunA17_Chr4g0040391 58.296 223 60 5 1 221 1 192 3.55e-81 252
Msa0528040 MtrunA17_Chr6g0487481 67.883 137 32 2 55 179 70 206 3.39e-66 215
Msa0528040 MtrunA17_Chr8g0389561 37.604 359 171 10 62 409 59 375 3.51e-63 207
Msa0528040 MtrunA17_Chr7g0257831 68.966 116 36 0 72 187 260 375 2.26e-57 200
Msa0528040 MtrunA17_Chr3g0088521 45.852 229 96 6 72 289 260 471 6.61e-56 196
Msa0528040 MtrunA17_Chr3g0135351 55.769 156 64 3 29 179 2 157 1.08e-54 182
Msa0528040 MtrunA17_Chr1g0171151 64.348 115 41 0 72 186 240 354 7.47e-52 183
Msa0528040 MtrunA17_Chr5g0437251 65.217 115 38 1 72 186 213 325 8.43e-51 181
Msa0528040 MtrunA17_Chr5g0430211 61.207 116 45 0 72 187 226 341 1.05e-50 181
Msa0528040 MtrunA17_Chr1g0187591 61.207 116 45 0 72 187 225 340 2.46e-49 177
Msa0528040 MtrunA17_Chr3g0092221 61.207 116 45 0 72 187 154 269 1.56e-48 170
Msa0528040 MtrunA17_Chr3g0105141 56.140 114 49 1 68 180 28 141 2.77e-45 154
Msa0528040 MtrunA17_Chr3g0113651 56.034 116 51 0 72 187 159 274 5.66e-45 162
Msa0528040 MtrunA17_Chr3g0092171 59.813 107 43 0 72 178 271 377 6.54e-43 157
Msa0528040 MtrunA17_Chr3g0105121 50.877 114 55 1 75 187 8 121 9.24e-37 137
Msa0528040 MtrunA17_Chr3g0105131 50.893 112 41 1 69 180 173 270 2.70e-33 130
Msa0528040 MtrunA17_Chr3g0105101 62.162 74 28 0 107 180 195 268 7.83e-30 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0528040 AT1G03790.1 46.006 363 137 12 65 409 71 392 3.66e-85 265
Msa0528040 AT5G44260.1 42.677 396 181 12 29 409 16 380 3.10e-78 247
Msa0528040 AT2G19810.1 67.347 147 38 1 55 191 47 193 9.51e-71 227
Msa0528040 AT4G29190.1 35.507 414 177 13 23 410 7 356 3.60e-69 223
Msa0528040 AT2G25900.2 74.561 114 29 0 70 183 84 197 3.44e-65 211
Msa0528040 AT2G25900.1 74.561 114 29 0 70 183 84 197 3.44e-65 211
Msa0528040 AT2G41900.1 67.241 116 38 0 72 187 257 372 3.96e-56 197
Msa0528040 AT5G58620.1 66.957 115 37 1 72 185 205 319 2.87e-54 190
Msa0528040 AT5G07500.1 68.182 110 35 0 69 178 45 154 2.20e-52 176
Msa0528040 AT5G12850.1 65.217 115 39 1 72 185 253 367 5.20e-52 185
Msa0528040 AT3G55980.1 39.526 253 101 6 27 268 156 367 4.00e-49 176
Msa0528040 AT2G40140.2 40.171 234 92 5 72 291 210 409 5.36e-49 175
Msa0528040 AT2G40140.1 40.171 234 92 5 72 291 210 409 5.36e-49 175
Msa0528040 AT3G55980.2 39.526 253 101 6 27 268 223 434 9.50e-49 176

Find 82 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCGGGTGAGGAGCTTTCT+TGG 0.183749 4_1:-724435 None:intergenic
AGACATGCTTATTGATCTTT+TGG 0.193333 4_1:+725169 Msa0528040:CDS
CATTGTGTGGGAGGAACTTT+TGG 0.221739 4_1:-724503 None:intergenic
GTTGGTGGTGGTGACGTTAA+TGG 0.315506 4_1:+725497 Msa0528040:CDS
GAGGGATGTCGATGTCTCTA+AGG 0.318194 4_1:-724413 None:intergenic
TTTGAAGATGATGATGTAAC+AGG 0.341921 4_1:-724879 None:intergenic
AAACAGCGACAATATTAATA+AGG 0.368587 4_1:+725472 Msa0528040:CDS
CGGTGGTGTTGCGGCGGGTG+AGG 0.397490 4_1:-724446 None:intergenic
TCATTCACCCATCCGAGATC+AGG 0.400183 4_1:-725536 None:intergenic
GACAATATTAATAAGGTTGT+TGG 0.414119 4_1:+725479 Msa0528040:CDS
TTTGGGAGAGAGAGGTTGGT+AGG 0.420760 4_1:-724385 None:intergenic
CTCGTAGCCGGAGCCATGAC+TGG 0.421786 4_1:+724601 Msa0528040:CDS
CGCCGCGGTGGCTGTAACCG+TGG 0.429136 4_1:+724714 Msa0528040:CDS
CAGCCACCGCGGCGATACTC+AGG 0.436266 4_1:-724705 None:intergenic
TCTTGTCCTGCCCCTGATCT+CGG 0.437151 4_1:+725524 Msa0528040:CDS
AGTGCTTTAGCAGCAGAAAC+TGG 0.447660 4_1:-725242 None:intergenic
TGTTGCTGATTCTGTTCCTC+AGG 0.458860 4_1:-725305 None:intergenic
GGACTTTGAATTCATACATT+CGG 0.463131 4_1:-724569 None:intergenic
CGGCGGCGGTGGTGTTGCGG+CGG 0.463780 4_1:-724452 None:intergenic
GGCGGCGGTGGTGTTGCGGC+GGG 0.472525 4_1:-724451 None:intergenic
GTCCTGCCCCTGATCTCGGA+TGG 0.476419 4_1:+725528 Msa0528040:CDS
GAAGATGTCGACGGCGGCGG+TGG 0.485634 4_1:-724463 None:intergenic
CGGGTGAGGAGCTTTCTTGG+AGG 0.487835 4_1:-724432 None:intergenic
CGGAAATGATCTGATGAGTA+AGG 0.490767 4_1:-724549 None:intergenic
AGAGAAGATGTCGACGGCGG+CGG 0.491138 4_1:-724466 None:intergenic
GGGTGAGGAGCTTTCTTGGA+GGG 0.496924 4_1:-724431 None:intergenic
TCCTGCCCCTGATCTCGGAT+GGG 0.499250 4_1:+725529 Msa0528040:CDS
ATTAATAAGGTTGTTGGTGG+TGG 0.500154 4_1:+725485 Msa0528040:CDS
AGAACTGAAGCATGCAAAGA+TGG 0.503562 4_1:+724795 Msa0528040:CDS
TCCGCCGGTGCACTCGTAGC+CGG 0.504444 4_1:+724589 Msa0528040:CDS
ACACCGTTACGTGGCTTCAT+CGG 0.504782 4_1:-725092 None:intergenic
GAACCATAGCGTGAGAGCGA+TGG 0.517489 4_1:-725116 None:intergenic
CGACGGCGGCGGTGGTGTTG+CGG 0.519055 4_1:-724455 None:intergenic
CGTGGCTTCATCGGAGACAT+AGG 0.522416 4_1:-725083 None:intergenic
CCACGGCGGTTTCATTACTC+AGG 0.527158 4_1:+724672 Msa0528040:CDS
CATTCACCCATCCGAGATCA+GGG 0.528306 4_1:-725535 None:intergenic
TTGTCGACATCATTGAAACT+TGG 0.531596 4_1:-725413 None:intergenic
GTTCCATCGCTCTCACGCTA+TGG 0.533114 4_1:+725113 Msa0528040:CDS
CACTGTGGCGGTAATGGTAA+TGG 0.535187 4_1:+724987 Msa0528040:CDS
ATCTGATGAGTAAGGATCAT+CGG 0.544740 4_1:-724541 None:intergenic
AATATTAATAAGGTTGTTGG+TGG 0.545047 4_1:+725482 Msa0528040:CDS
GCTTCAGTTCTGTATCTAGC+AGG 0.547143 4_1:-724783 None:intergenic
GAAGGGATGAATTTCAATGA+CGG 0.555565 4_1:+725200 Msa0528040:CDS
GGCGGTAATGGTAATGGTAA+TGG 0.562964 4_1:+724993 Msa0528040:CDS
AATGAAACCGCCGTGGATCA+CGG 0.566037 4_1:-724665 None:intergenic
AAGCTCGCCGCCGTGATCCA+CGG 0.567851 4_1:+724655 Msa0528040:CDS
AGTCAGTCCAGTCATGGCTC+CGG 0.569804 4_1:-724608 None:intergenic
TGATCAAAAGATGAAGAAGA+AGG 0.576719 4_1:-725341 None:intergenic
GGAGACATAGGTGGTGATGC+CGG 0.581592 4_1:-725071 None:intergenic
GATACTTGTGAATTTGCTCA+TGG 0.582670 4_1:+724738 Msa0528040:CDS
ATGGACAGTCAGTCCAGTCA+TGG 0.586583 4_1:-724614 None:intergenic
TGAATCTCTTGATGTTCCTG+AGG 0.587490 4_1:+725289 Msa0528040:CDS
AGCCACCGCGGCGATACTCA+GGG 0.587575 4_1:-724704 None:intergenic
ACCCATCCGAGATCAGGGGC+AGG 0.589319 4_1:-725530 None:intergenic
GACTGTCCATTCGCTCACCC+CGG 0.597229 4_1:+724627 Msa0528040:CDS
AGCACTTCAGATACAAAACA+TGG 0.598969 4_1:+725259 Msa0528040:CDS
TCTCCGATGAAGCCACGTAA+CGG 0.601715 4_1:+725089 Msa0528040:CDS
AGGGATGTCGATGTCTCTAA+GGG 0.602570 4_1:-724412 None:intergenic
TCCGGCTACGAGTGCACCGG+CGG 0.607263 4_1:-724590 None:intergenic
AAGATGATGATGTAACAGGA+AGG 0.616658 4_1:-724875 None:intergenic
AGTCAGAGTCATTGTGTGGG+AGG 0.622332 4_1:-724512 None:intergenic
TCTGAGTCAGAGTCATTGTG+TGG 0.623962 4_1:-724516 None:intergenic
GGCTCCGGCTACGAGTGCAC+CGG 0.627207 4_1:-724593 None:intergenic
TGCCCTGAGTATCGCCGCGG+TGG 0.630381 4_1:+724702 Msa0528040:CDS
CACGGCGGTTTCATTACTCA+GGG 0.632004 4_1:+724673 Msa0528040:CDS
CTGAGTCAGAGTCATTGTGT+GGG 0.635621 4_1:-724515 None:intergenic
CAAATTCACAAGTATCGCCA+CGG 0.650826 4_1:-724731 None:intergenic
AGCGATGGAACACCGTTACG+TGG 0.658393 4_1:-725101 None:intergenic
AGGAAGAATGAAACTTGTGT+TGG 0.664057 4_1:-724365 None:intergenic
GACATAGGTGGTGATGCCGG+AGG 0.666150 4_1:-725068 None:intergenic
GGAAGGATTCTTAATTGACG+AGG 0.669701 4_1:-724858 None:intergenic
TGTATGAATTCAAAGTCCGC+CGG 0.674896 4_1:+724574 Msa0528040:CDS
GTCGTCAGAGAAGATGTCGA+CGG 0.679038 4_1:-724472 None:intergenic
ATTCACCCATCCGAGATCAG+GGG 0.681023 4_1:-725534 None:intergenic
GGCTTCATCGGAGACATAGG+TGG 0.681714 4_1:-725080 None:intergenic
CCTGAGTAATGAAACCGCCG+TGG 0.683137 4_1:-724672 None:intergenic
GTCAGAGAAGATGTCGACGG+CGG 0.689427 4_1:-724469 None:intergenic
CTCGCCGCCGTGATCCACGG+CGG 0.698305 4_1:+724658 Msa0528040:CDS
GCACTTCAGATACAAAACAT+GGG 0.708790 4_1:+725260 Msa0528040:CDS
CGCCACGGTTACAGCCACCG+CGG 0.740750 4_1:-724716 None:intergenic
GAAACCGCCGTGGATCACGG+CGG 0.763141 4_1:-724662 None:intergenic
GTTTGCCCTGAGTATCGCCG+CGG 0.768550 4_1:+724699 Msa0528040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATATGAACATGTTTTT+GGG + chr4_1:725449-725468 Msa0528040:CDS 20.0%
!! CAACAATATGAACATGTTTT+TGG + chr4_1:725448-725467 Msa0528040:CDS 25.0%
! AAACAGCGACAATATTAATA+AGG + chr4_1:725472-725491 Msa0528040:CDS 25.0%
! GACAATATTAATAAGGTTGT+TGG + chr4_1:725479-725498 Msa0528040:CDS 25.0%
! AATATTAATAAGGTTGTTGG+TGG + chr4_1:725482-725501 Msa0528040:CDS 25.0%
!!! ATGTCTCTAAGGGATTTTTT+GGG - chr4_1:724405-724424 None:intergenic 30.0%
GGACTTTGAATTCATACATT+CGG - chr4_1:724572-724591 None:intergenic 30.0%
! TTTGAAGATGATGATGTAAC+AGG - chr4_1:724882-724901 None:intergenic 30.0%
!!! TGTTTGATTTTGAAGCAACA+TGG - chr4_1:724950-724969 None:intergenic 30.0%
!! ATACCAAAAAGTGTTGAAGT+TGG - chr4_1:725032-725051 None:intergenic 30.0%
! AGACATGCTTATTGATCTTT+TGG + chr4_1:725169-725188 Msa0528040:CDS 30.0%
! GACATGCTTATTGATCTTTT+GGG + chr4_1:725170-725189 Msa0528040:CDS 30.0%
!!! ATCTTTTGGGTTCTTTTGAA+GGG + chr4_1:725183-725202 Msa0528040:CDS 30.0%
TGATCAAAAGATGAAGAAGA+AGG - chr4_1:725344-725363 None:intergenic 30.0%
AGGAAGAATGAAACTTGTGT+TGG - chr4_1:724368-724387 None:intergenic 35.0%
!!! GATGTCTCTAAGGGATTTTT+TGG - chr4_1:724406-724425 None:intergenic 35.0%
ATCTGATGAGTAAGGATCAT+CGG - chr4_1:724544-724563 None:intergenic 35.0%
! GATACTTGTGAATTTGCTCA+TGG + chr4_1:724738-724757 Msa0528040:CDS 35.0%
! AAGATGATGATGTAACAGGA+AGG - chr4_1:724878-724897 None:intergenic 35.0%
!!! TGTTGTTTGTTTTGTCACTG+TGG + chr4_1:724972-724991 Msa0528040:CDS 35.0%
! TCTCCAACTTCAACACTTTT+TGG + chr4_1:725026-725045 Msa0528040:CDS 35.0%
!!! GATCTTTTGGGTTCTTTTGA+AGG + chr4_1:725182-725201 Msa0528040:CDS 35.0%
GAAGGGATGAATTTCAATGA+CGG + chr4_1:725200-725219 Msa0528040:CDS 35.0%
AGCACTTCAGATACAAAACA+TGG + chr4_1:725259-725278 Msa0528040:CDS 35.0%
GCACTTCAGATACAAAACAT+GGG + chr4_1:725260-725279 Msa0528040:CDS 35.0%
TTGTCGACATCATTGAAACT+TGG - chr4_1:725416-725435 None:intergenic 35.0%
! ATTAATAAGGTTGTTGGTGG+TGG + chr4_1:725485-725504 Msa0528040:CDS 35.0%
!!! ATTTTTTGGGAGAGAGAGGT+TGG - chr4_1:724392-724411 None:intergenic 40.0%
CGGAAATGATCTGATGAGTA+AGG - chr4_1:724552-724571 None:intergenic 40.0%
TGTATGAATTCAAAGTCCGC+CGG + chr4_1:724574-724593 Msa0528040:CDS 40.0%
! CAAATTCACAAGTATCGCCA+CGG - chr4_1:724734-724753 None:intergenic 40.0%
!! CTCATGGTGTTTTTGAATGC+TGG + chr4_1:724754-724773 Msa0528040:CDS 40.0%
AGAACTGAAGCATGCAAAGA+TGG + chr4_1:724795-724814 Msa0528040:CDS 40.0%
GGAAGGATTCTTAATTGACG+AGG - chr4_1:724861-724880 None:intergenic 40.0%
!!! TGTTTGTTTTGTCACTGTGG+CGG + chr4_1:724975-724994 Msa0528040:CDS 40.0%
!! TTCTCATTTTTCTTCGCCTC+CGG + chr4_1:725052-725071 Msa0528040:CDS 40.0%
TGAATCTCTTGATGTTCCTG+AGG + chr4_1:725289-725308 Msa0528040:CDS 40.0%
!!! AGGGATTTTTTGGGAGAGAG+AGG - chr4_1:724396-724415 None:intergenic 45.0%
AGGGATGTCGATGTCTCTAA+GGG - chr4_1:724415-724434 None:intergenic 45.0%
! CATTGTGTGGGAGGAACTTT+TGG - chr4_1:724506-724525 None:intergenic 45.0%
CTGAGTCAGAGTCATTGTGT+GGG - chr4_1:724518-724537 None:intergenic 45.0%
TCTGAGTCAGAGTCATTGTG+TGG - chr4_1:724519-724538 None:intergenic 45.0%
GCTTCAGTTCTGTATCTAGC+AGG - chr4_1:724786-724805 None:intergenic 45.0%
! TTTTGTCACTGTGGCGGTAA+TGG + chr4_1:724981-725000 Msa0528040:CDS 45.0%
!! GGCGGTAATGGTAATGGTAA+TGG + chr4_1:724993-725012 Msa0528040:CDS 45.0%
AGTGCTTTAGCAGCAGAAAC+TGG - chr4_1:725245-725264 None:intergenic 45.0%
!! TGTTGCTGATTCTGTTCCTC+AGG - chr4_1:725308-725327 None:intergenic 45.0%
! TTTGGGAGAGAGAGGTTGGT+AGG - chr4_1:724388-724407 None:intergenic 50.0%
GAGGGATGTCGATGTCTCTA+AGG - chr4_1:724416-724435 None:intergenic 50.0%
GTCGTCAGAGAAGATGTCGA+CGG - chr4_1:724475-724494 None:intergenic 50.0%
!! TGGGAGGAACTTTTGGAGGA+TGG - chr4_1:724499-724518 None:intergenic 50.0%
! TGTGTGGGAGGAACTTTTGG+AGG - chr4_1:724503-724522 None:intergenic 50.0%
AGTCAGAGTCATTGTGTGGG+AGG - chr4_1:724515-724534 None:intergenic 50.0%
ATGGACAGTCAGTCCAGTCA+TGG - chr4_1:724617-724636 None:intergenic 50.0%
AATGAAACCGCCGTGGATCA+CGG - chr4_1:724668-724687 None:intergenic 50.0%
CACGGCGGTTTCATTACTCA+GGG + chr4_1:724673-724692 Msa0528040:CDS 50.0%
! CACTGTGGCGGTAATGGTAA+TGG + chr4_1:724987-725006 Msa0528040:CDS 50.0%
TCTCCGATGAAGCCACGTAA+CGG + chr4_1:725089-725108 Msa0528040:CDS 50.0%
ACACCGTTACGTGGCTTCAT+CGG - chr4_1:725095-725114 None:intergenic 50.0%
!! GTTGGTGGTGGTGACGTTAA+TGG + chr4_1:725497-725516 Msa0528040:CDS 50.0%
ATTCACCCATCCGAGATCAG+GGG - chr4_1:725537-725556 None:intergenic 50.0%
CATTCACCCATCCGAGATCA+GGG - chr4_1:725538-725557 None:intergenic 50.0%
TCATTCACCCATCCGAGATC+AGG - chr4_1:725539-725558 None:intergenic 50.0%
GGGTGAGGAGCTTTCTTGGA+GGG - chr4_1:724434-724453 None:intergenic 55.0%
GTCAGAGAAGATGTCGACGG+CGG - chr4_1:724472-724491 None:intergenic 55.0%
AGTCAGTCCAGTCATGGCTC+CGG - chr4_1:724611-724630 None:intergenic 55.0%
! TTTTCACCGGGGTGAGCGAA+TGG - chr4_1:724636-724655 None:intergenic 55.0%
CCTGAGTAATGAAACCGCCG+TGG - chr4_1:724675-724694 None:intergenic 55.0%
CCACGGCGGTTTCATTACTC+AGG + chr4_1:724672-724691 Msa0528040:CDS 55.0%
! GGAGACATAGGTGGTGATGC+CGG - chr4_1:725074-725093 None:intergenic 55.0%
GGCTTCATCGGAGACATAGG+TGG - chr4_1:725083-725102 None:intergenic 55.0%
CGTGGCTTCATCGGAGACAT+AGG - chr4_1:725086-725105 None:intergenic 55.0%
AGCGATGGAACACCGTTACG+TGG - chr4_1:725104-725123 None:intergenic 55.0%
GTTCCATCGCTCTCACGCTA+TGG + chr4_1:725113-725132 Msa0528040:CDS 55.0%
GAACCATAGCGTGAGAGCGA+TGG - chr4_1:725119-725138 None:intergenic 55.0%
TCTTGTCCTGCCCCTGATCT+CGG + chr4_1:725524-725543 Msa0528040:CDS 55.0%
CGGGTGAGGAGCTTTCTTGG+AGG - chr4_1:724435-724454 None:intergenic 60.0%
AGAGAAGATGTCGACGGCGG+CGG - chr4_1:724469-724488 None:intergenic 60.0%
GACTGTCCATTCGCTCACCC+CGG + chr4_1:724627-724646 Msa0528040:CDS 60.0%
!!! ACGGCGGCGAGCTTTTTCAC+CGG - chr4_1:724649-724668 None:intergenic 60.0%
! GTTTGCCCTGAGTATCGCCG+CGG + chr4_1:724699-724718 Msa0528040:CDS 60.0%
! GACATAGGTGGTGATGCCGG+AGG - chr4_1:725071-725090 None:intergenic 60.0%
TCCTGCCCCTGATCTCGGAT+GGG + chr4_1:725529-725548 Msa0528040:CDS 60.0%
CGGCGGGTGAGGAGCTTTCT+TGG - chr4_1:724438-724457 None:intergenic 65.0%
CTCGTAGCCGGAGCCATGAC+TGG + chr4_1:724601-724620 Msa0528040:CDS 65.0%
!!! GGCGGCGAGCTTTTTCACCG+GGG - chr4_1:724647-724666 None:intergenic 65.0%
!!! CGGCGGCGAGCTTTTTCACC+GGG - chr4_1:724648-724667 None:intergenic 65.0%
AAGCTCGCCGCCGTGATCCA+CGG + chr4_1:724655-724674 Msa0528040:CDS 65.0%
GAAACCGCCGTGGATCACGG+CGG - chr4_1:724665-724684 None:intergenic 65.0%
AGCCACCGCGGCGATACTCA+GGG - chr4_1:724707-724726 None:intergenic 65.0%
GTCCTGCCCCTGATCTCGGA+TGG + chr4_1:725528-725547 Msa0528040:CDS 65.0%
ACCCATCCGAGATCAGGGGC+AGG - chr4_1:725533-725552 None:intergenic 65.0%
GAAGATGTCGACGGCGGCGG+TGG - chr4_1:724466-724485 None:intergenic 70.0%
TCCGCCGGTGCACTCGTAGC+CGG + chr4_1:724589-724608 Msa0528040:CDS 70.0%
TCCGGCTACGAGTGCACCGG+CGG - chr4_1:724593-724612 None:intergenic 70.0%
GGCTCCGGCTACGAGTGCAC+CGG - chr4_1:724596-724615 None:intergenic 70.0%
! TGCCCTGAGTATCGCCGCGG+TGG + chr4_1:724702-724721 Msa0528040:CDS 70.0%
CAGCCACCGCGGCGATACTC+AGG - chr4_1:724708-724727 None:intergenic 70.0%
CGCCACGGTTACAGCCACCG+CGG - chr4_1:724719-724738 None:intergenic 70.0%
!! CGGTGGTGTTGCGGCGGGTG+AGG - chr4_1:724449-724468 None:intergenic 75.0%
! CGACGGCGGCGGTGGTGTTG+CGG - chr4_1:724458-724477 None:intergenic 75.0%
CTCGCCGCCGTGATCCACGG+CGG + chr4_1:724658-724677 Msa0528040:CDS 75.0%
CGCCGCGGTGGCTGTAACCG+TGG + chr4_1:724714-724733 Msa0528040:CDS 75.0%
!!! GGCGGCGGTGGTGTTGCGGC+GGG - chr4_1:724454-724473 None:intergenic 80.0%
!!! CGGCGGCGGTGGTGTTGCGG+CGG - chr4_1:724455-724474 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 724339 725571 724339 ID=Msa0528040;Name=Msa0528040
chr4_1 mRNA 724339 725571 724339 ID=Msa0528040-mRNA-1;Parent=Msa0528040;Name=Msa0528040-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|410
chr4_1 exon 724339 725571 724339 ID=Msa0528040-mRNA-1:exon:149;Parent=Msa0528040-mRNA-1
chr4_1 CDS 724339 725571 724339 ID=Msa0528040-mRNA-1:cds;Parent=Msa0528040-mRNA-1
Gene Sequence

>Msa0528040

ATGACTACTATTTGTGCTGAACAACACCAACACAAGTTTCATTCTTCCTACCAACCTCTCTCTCCCAAAAAATCCCTTAGAGACATCGACATCCCTCCAAGAAAGCTCCTCACCCGCCGCAACACCACCGCCGCCGTCGACATCTTCTCTGACGACACCATCCTCCAAAAGTTCCTCCCACACAATGACTCTGACTCAGACTCCGATGATCCTTACTCATCAGATCATTTCCGAATGTATGAATTCAAAGTCCGCCGGTGCACTCGTAGCCGGAGCCATGACTGGACTGACTGTCCATTCGCTCACCCCGGTGAAAAAGCTCGCCGCCGTGATCCACGGCGGTTTCATTACTCAGGGACAGTTTGCCCTGAGTATCGCCGCGGTGGCTGTAACCGTGGCGATACTTGTGAATTTGCTCATGGTGTTTTTGAATGCTGGCTTCATCCTGCTAGATACAGAACTGAAGCATGCAAAGATGGTAAAAACTGTAAGAGAAAAGTTTGTTTCTTTGCACACACTCCTCGTCAATTAAGAATCCTTCCTGTTACATCATCATCTTCAAATGAACATTTTTCATGCAACAAGAAAAATAACAAGCTTTTGAATAACCATGTTGCTTCAAAATCAAACAATTGTTGTTTGTTTTGTCACTGTGGCGGTAATGGTAATGGTAATGGTGCTAATAATTCTCCAACTTCAACACTTTTTGGTATTTCTCATTTTTCTTCGCCTCCGGCATCACCACCTATGTCTCCGATGAAGCCACGTAACGGTGTTCCATCGCTCTCACGCTATGGTTCTTCGATTAATAGTAACAATCTTCGTTATAGAGACATGCTTATTGATCTTTTGGGTTCTTTTGAAGGGATGAATTTCAATGACGGTTCATCTTCTTCTTCTTTACCAGTTTCTGCTGCTAAAGCACTTCAGATACAAAACATGGGTTATCTTGAATCTCTTGATGTTCCTGAGGAACAGAATCAGCAACAGTTTATTGTTTCACCTTCTTCTTCATCTTTTGATCATCAACAACATTTCAATATTCTTTCACAATCTATTCATCAGAATTTGACACCAAGTTTCAATGATGTCGACAATAACATCATTAACAACAATATGAACATGTTTTTGGGAAACAGCGACAATATTAATAAGGTTGTTGGTGGTGGTGACGTTAATGGTTTGTCTTGTCCTGCCCCTGATCTCGGATGGGTGAATGAATTGCTGATGTAG
Protein sequence

>Msa0528040

MTTICAEQHQHKFHSSYQPLSPKKSLRDIDIPPRKLLTRRNTTAAVDIFSDDTILQKFLPHNDSDSDSDDPYSSDHFRMYEFKVRRCTRSRSHDWTDCPFAHPGEKARRRDPRRFHYSGTVCPEYRRGGCNRGDTCEFAHGVFECWLHPARYRTEACKDGKNCKRKVCFFAHTPRQLRILPVTSSSSNEHFSCNKKNNKLLNNHVASKSNNCCLFCHCGGNGNGNGANNSPTSTLFGISHFSSPPASPPMSPMKPRNGVPSLSRYGSSINSNNLRYRDMLIDLLGSFEGMNFNDGSSSSSLPVSAAKALQIQNMGYLESLDVPEEQNQQQFIVSPSSSSFDHQQHFNILSQSIHQNLTPSFNDVDNNIINNNMNMFLGNSDNINKVVGGGDVNGLSCPAPDLGWVNELLM*