AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0746090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0746090 MtrunA17_Chr4g0075751 92.143 140 10 1 1 139 1 140 1.45e-40 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACTGTTACTACTTCTT+CGG 0.326738 5_1:+68089715 None:intergenic
TTACCTCAACAGTGGCCATT+TGG 0.390008 5_1:+68090327 None:intergenic
GTGGTGGTTACTTCTTCAAC+TGG 0.408262 5_1:+68089455 None:intergenic
CGGGTTCTCTACCTCTGCCT+TGG 0.417414 5_1:+68089637 None:intergenic
TTCTTCAACTGGTGCTGCTA+TGG 0.423717 5_1:+68089466 None:intergenic
TGTGTACTCTCAGCTTCAAC+TGG 0.435451 5_1:+68089674 None:intergenic
GAAATATGAACTAGCAAGAA+AGG 0.445512 5_1:+68090373 None:intergenic
ACTACTTCTTCGGTGGTGGC+TGG 0.451704 5_1:+68089725 None:intergenic
TGTTACTACTTCTTCGGTGG+TGG 0.470019 5_1:+68089721 None:intergenic
AGAAACTACTGAAACAGAAC+CGG 0.474732 5_1:-68089564 Msa0746090:CDS
ATTCATCTTTCCATTCCAAA+TGG 0.477645 5_1:-68090342 Msa0746090:exon
CAAGGCAGAGGTAGAGAACC+CGG 0.480116 5_1:-68089636 Msa0746090:CDS
CGGTGTTGTTTCCTCGACCT+TGG 0.487832 5_1:+68089772 None:intergenic
CACTGTTACTACTTCTTCGG+TGG 0.532727 5_1:+68089718 None:intergenic
GGTGGTGGCTGGTTCTGAAG+TGG 0.534204 5_1:+68089736 None:intergenic
AGAGGAAGTATCAGCTGAAG+AGG 0.536609 5_1:-68089591 Msa0746090:CDS
ATTCCAAATGGCCACTGTTG+AGG 0.536707 5_1:-68090330 Msa0746090:intron
AACTGAGGAAGCCAAGGCAG+AGG 0.538217 5_1:-68089648 Msa0746090:CDS
GAAGTAGTAACAGTGGAGAA+AGG 0.540973 5_1:-68089710 Msa0746090:CDS
TCAACAGTGGCCATTTGGAA+TGG 0.543349 5_1:+68090332 None:intergenic
GTGGTGGCTGGTTCTGAAGT+GGG 0.546159 5_1:+68089737 None:intergenic
GAAGTGGGAACCTCGGAGGC+CGG 0.549296 5_1:+68089752 None:intergenic
AGAGGAAGTATCAACTGAAG+AGG 0.587702 5_1:-68089519 Msa0746090:CDS
TGGTTCTGAAGTGGGAACCT+CGG 0.596316 5_1:+68089745 None:intergenic
AGTTGAGGTAACCAAGGTCG+AGG 0.604857 5_1:-68089783 Msa0746090:CDS
GACAGAAAATGAAACAGTTG+AGG 0.607247 5_1:-68089798 Msa0746090:CDS
ACAAGTAACTGAGGAAGCCA+AGG 0.616520 5_1:-68089654 Msa0746090:CDS
TGAGAGTACACAAGTAACTG+AGG 0.633074 5_1:-68089663 Msa0746090:CDS
TGAAACAGTTGAGGTAACCA+AGG 0.643241 5_1:-68089789 Msa0746090:CDS
TTCTGAAGTGGGAACCTCGG+AGG 0.645606 5_1:+68089748 None:intergenic
GGAAACAACACCGGCCTCCG+AGG 0.649162 5_1:-68089762 Msa0746090:CDS
CACCGAAGAAGTAGTAACAG+TGG 0.674611 5_1:-68089717 Msa0746090:CDS
TCTTTGAATTACCTCAACAG+TGG 0.681859 5_1:+68090319 None:intergenic
CAAGGTCGAGGAAACAACAC+CGG 0.700669 5_1:-68089771 Msa0746090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATTATTGATCTTTTT+TGG + chr5_1:68089804-68089823 None:intergenic 15.0%
!!! AATTGATTATTGATCTTTTT+TGG + chr5_1:68089804-68089823 None:intergenic 15.0%
!! AAACAAAAATATTAGTCCAA+TGG - chr5_1:68089724-68089743 Msa0746090:CDS 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG + chr5_1:68089743-68089762 None:intergenic 20.0%
!! TGGATTATGAAAAATAAACA+CGG + chr5_1:68089784-68089803 None:intergenic 20.0%
!! AAACAAAAATATTAGTCCAA+TGG - chr5_1:68089724-68089743 Msa0746090:CDS 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG + chr5_1:68089743-68089762 None:intergenic 20.0%
!! TGGATTATGAAAAATAAACA+CGG + chr5_1:68089784-68089803 None:intergenic 20.0%
!!! AATATTTTTGTTTGATCCAC+TGG + chr5_1:68089717-68089736 None:intergenic 25.0%
! GATTATGAAAAATAAACACG+GGG + chr5_1:68089782-68089801 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+GGG + chr5_1:68089783-68089802 None:intergenic 25.0%
!!! AATATTTTTGTTTGATCCAC+TGG + chr5_1:68089717-68089736 None:intergenic 25.0%
! GATTATGAAAAATAAACACG+GGG + chr5_1:68089782-68089801 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+GGG + chr5_1:68089783-68089802 None:intergenic 25.0%
GAAATATGAACTAGCAAGAA+AGG + chr5_1:68089485-68089504 None:intergenic 30.0%
ATTCATCTTTCCATTCCAAA+TGG - chr5_1:68089513-68089532 Msa0746090:CDS 30.0%
CTTGATGTTACAAATTCACA+CGG - chr5_1:68089685-68089704 Msa0746090:CDS 30.0%
ATATGACCAAGTAACTCATA+GGG + chr5_1:68089957-68089976 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG + chr5_1:68089958-68089977 None:intergenic 30.0%
!! TCTTTCACTTCAACTTTTTC+AGG + chr5_1:68090247-68090266 None:intergenic 30.0%
TGAAAAAGTTGAAGTGAAAG+AGG - chr5_1:68090246-68090265 Msa0746090:intron 30.0%
GAAATATGAACTAGCAAGAA+AGG + chr5_1:68089485-68089504 None:intergenic 30.0%
ATTCATCTTTCCATTCCAAA+TGG - chr5_1:68089513-68089532 Msa0746090:CDS 30.0%
CTTGATGTTACAAATTCACA+CGG - chr5_1:68089685-68089704 Msa0746090:CDS 30.0%
ATATGACCAAGTAACTCATA+GGG + chr5_1:68089957-68089976 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG + chr5_1:68089958-68089977 None:intergenic 30.0%
!! TCTTTCACTTCAACTTTTTC+AGG + chr5_1:68090247-68090266 None:intergenic 30.0%
TGAAAAAGTTGAAGTGAAAG+AGG - chr5_1:68090246-68090265 Msa0746090:intron 30.0%
TCTTTGAATTACCTCAACAG+TGG + chr5_1:68089539-68089558 None:intergenic 35.0%
CATAACAACATGTTGAGCAT+TGG - chr5_1:68089980-68089999 Msa0746090:intron 35.0%
! GCATTGGTGGTTAATGAAAA+TGG - chr5_1:68089996-68090015 Msa0746090:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG - chr5_1:68090006-68090025 Msa0746090:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG + chr5_1:68090049-68090068 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG - chr5_1:68090057-68090076 Msa0746090:intron 35.0%
!!! ACTTCAACTTTTTCAGGTTC+CGG + chr5_1:68090241-68090260 None:intergenic 35.0%
AGAAACTACTGAAACAGAAC+CGG - chr5_1:68090291-68090310 Msa0746090:intron 35.0%
!! TCTTTTGCTTCGACTTTTTC+GGG + chr5_1:68090319-68090338 None:intergenic 35.0%
! CTCTTTTGCTTCGACTTTTT+CGG + chr5_1:68090320-68090339 None:intergenic 35.0%
TCTTTGAATTACCTCAACAG+TGG + chr5_1:68089539-68089558 None:intergenic 35.0%
CATAACAACATGTTGAGCAT+TGG - chr5_1:68089980-68089999 Msa0746090:intron 35.0%
! GCATTGGTGGTTAATGAAAA+TGG - chr5_1:68089996-68090015 Msa0746090:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG - chr5_1:68090006-68090025 Msa0746090:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG + chr5_1:68090049-68090068 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG - chr5_1:68090057-68090076 Msa0746090:intron 35.0%
!!! ACTTCAACTTTTTCAGGTTC+CGG + chr5_1:68090241-68090260 None:intergenic 35.0%
AGAAACTACTGAAACAGAAC+CGG - chr5_1:68090291-68090310 Msa0746090:intron 35.0%
!! TCTTTTGCTTCGACTTTTTC+GGG + chr5_1:68090319-68090338 None:intergenic 35.0%
! CTCTTTTGCTTCGACTTTTT+CGG + chr5_1:68090320-68090339 None:intergenic 35.0%
CATGTTCCCTATGAGTTACT+TGG - chr5_1:68089948-68089967 Msa0746090:intron 40.0%
!! AACAACATGTTGAGCATTGG+TGG - chr5_1:68089983-68090002 Msa0746090:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG - chr5_1:68090066-68090085 Msa0746090:intron 40.0%
CTCCACTGTTACTACTTCTT+CGG + chr5_1:68090143-68090162 None:intergenic 40.0%
GAAGTAGTAACAGTGGAGAA+AGG - chr5_1:68090145-68090164 Msa0746090:intron 40.0%
! TGAGAGTACACAAGTAACTG+AGG - chr5_1:68090192-68090211 Msa0746090:intron 40.0%
!!! CTTCAACTTTTTCAGGTTCC+GGG + chr5_1:68090240-68090259 None:intergenic 40.0%
CGAAAAAGTCGAAGCAAAAG+AGG - chr5_1:68090318-68090337 Msa0746090:intron 40.0%
!! AGAGGAAGTATCAACTGAAG+AGG - chr5_1:68090336-68090355 Msa0746090:exon 40.0%
CATGTTCCCTATGAGTTACT+TGG - chr5_1:68089948-68089967 Msa0746090:intron 40.0%
!! AACAACATGTTGAGCATTGG+TGG - chr5_1:68089983-68090002 Msa0746090:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG - chr5_1:68090066-68090085 Msa0746090:intron 40.0%
CTCCACTGTTACTACTTCTT+CGG + chr5_1:68090143-68090162 None:intergenic 40.0%
GAAGTAGTAACAGTGGAGAA+AGG - chr5_1:68090145-68090164 Msa0746090:intron 40.0%
! TGAGAGTACACAAGTAACTG+AGG - chr5_1:68090192-68090211 Msa0746090:intron 40.0%
!!! CTTCAACTTTTTCAGGTTCC+GGG + chr5_1:68090240-68090259 None:intergenic 40.0%
CGAAAAAGTCGAAGCAAAAG+AGG - chr5_1:68090318-68090337 Msa0746090:intron 40.0%
!! AGAGGAAGTATCAACTGAAG+AGG - chr5_1:68090336-68090355 Msa0746090:exon 40.0%
TCAACAGTGGCCATTTGGAA+TGG + chr5_1:68089526-68089545 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG - chr5_1:68089525-68089544 Msa0746090:CDS 45.0%
TTACCTCAACAGTGGCCATT+TGG + chr5_1:68089531-68089550 None:intergenic 45.0%
! ATTCACACGGCATTTTCCAG+TGG - chr5_1:68089698-68089717 Msa0746090:CDS 45.0%
! TTTGCTGTGTTTCAACCTGC+AGG + chr5_1:68090024-68090043 None:intergenic 45.0%
TGTTACTACTTCTTCGGTGG+TGG + chr5_1:68090137-68090156 None:intergenic 45.0%
CACTGTTACTACTTCTTCGG+TGG + chr5_1:68090140-68090159 None:intergenic 45.0%
CACCGAAGAAGTAGTAACAG+TGG - chr5_1:68090138-68090157 Msa0746090:intron 45.0%
TGTGTACTCTCAGCTTCAAC+TGG + chr5_1:68090184-68090203 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+AGG - chr5_1:68090201-68090220 Msa0746090:intron 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG - chr5_1:68090264-68090283 Msa0746090:intron 45.0%
!! TTCTTCAACTGGTGCTGCTA+TGG + chr5_1:68090392-68090411 None:intergenic 45.0%
GTGGTGGTTACTTCTTCAAC+TGG + chr5_1:68090403-68090422 None:intergenic 45.0%
TCAACAGTGGCCATTTGGAA+TGG + chr5_1:68089526-68089545 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG - chr5_1:68089525-68089544 Msa0746090:CDS 45.0%
TTACCTCAACAGTGGCCATT+TGG + chr5_1:68089531-68089550 None:intergenic 45.0%
! ATTCACACGGCATTTTCCAG+TGG - chr5_1:68089698-68089717 Msa0746090:CDS 45.0%
! TTTGCTGTGTTTCAACCTGC+AGG + chr5_1:68090024-68090043 None:intergenic 45.0%
TGTTACTACTTCTTCGGTGG+TGG + chr5_1:68090137-68090156 None:intergenic 45.0%
CACTGTTACTACTTCTTCGG+TGG + chr5_1:68090140-68090159 None:intergenic 45.0%
CACCGAAGAAGTAGTAACAG+TGG - chr5_1:68090138-68090157 Msa0746090:intron 45.0%
TGTGTACTCTCAGCTTCAAC+TGG + chr5_1:68090184-68090203 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+AGG - chr5_1:68090201-68090220 Msa0746090:intron 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG - chr5_1:68090264-68090283 Msa0746090:intron 45.0%
!! TTCTTCAACTGGTGCTGCTA+TGG + chr5_1:68090392-68090411 None:intergenic 45.0%
GTGGTGGTTACTTCTTCAAC+TGG + chr5_1:68090403-68090422 None:intergenic 45.0%
AGTTGAGGTAACCAAGGTCG+AGG - chr5_1:68090072-68090091 Msa0746090:intron 50.0%
CAAGGTCGAGGAAACAACAC+CGG - chr5_1:68090084-68090103 Msa0746090:intron 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG + chr5_1:68090113-68090132 None:intergenic 50.0%
AGTTGAGGTAACCAAGGTCG+AGG - chr5_1:68090072-68090091 Msa0746090:intron 50.0%
CAAGGTCGAGGAAACAACAC+CGG - chr5_1:68090084-68090103 Msa0746090:intron 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG + chr5_1:68090113-68090132 None:intergenic 50.0%
!! CGGTGTTGTTTCCTCGACCT+TGG + chr5_1:68090086-68090105 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG + chr5_1:68090110-68090129 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG + chr5_1:68090121-68090140 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG + chr5_1:68090133-68090152 None:intergenic 55.0%
AACTGAGGAAGCCAAGGCAG+AGG - chr5_1:68090207-68090226 Msa0746090:intron 55.0%
CAAGGCAGAGGTAGAGAACC+CGG - chr5_1:68090219-68090238 Msa0746090:intron 55.0%
!!! GCTTCGACTTTTTCGGGTGC+CGG + chr5_1:68090313-68090332 None:intergenic 55.0%
!! CGGTGTTGTTTCCTCGACCT+TGG + chr5_1:68090086-68090105 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG + chr5_1:68090110-68090129 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG + chr5_1:68090121-68090140 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG + chr5_1:68090133-68090152 None:intergenic 55.0%
AACTGAGGAAGCCAAGGCAG+AGG - chr5_1:68090207-68090226 Msa0746090:intron 55.0%
CAAGGCAGAGGTAGAGAACC+CGG - chr5_1:68090219-68090238 Msa0746090:intron 55.0%
!!! GCTTCGACTTTTTCGGGTGC+CGG + chr5_1:68090313-68090332 None:intergenic 55.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG + chr5_1:68090122-68090141 None:intergenic 60.0%
CGGGTTCTCTACCTCTGCCT+TGG + chr5_1:68090221-68090240 None:intergenic 60.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG + chr5_1:68090122-68090141 None:intergenic 60.0%
CGGGTTCTCTACCTCTGCCT+TGG + chr5_1:68090221-68090240 None:intergenic 60.0%
GGAAACAACACCGGCCTCCG+AGG - chr5_1:68090093-68090112 Msa0746090:intron 65.0%
GAAGTGGGAACCTCGGAGGC+CGG + chr5_1:68090106-68090125 None:intergenic 65.0%
GGAAACAACACCGGCCTCCG+AGG - chr5_1:68090093-68090112 Msa0746090:intron 65.0%
GAAGTGGGAACCTCGGAGGC+CGG + chr5_1:68090106-68090125 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 68089445 68090432 68089445 ID=Msa0746090;Name=Msa0746090
chr5_1 mRNA 68089445 68090432 68089445 ID=Msa0746090-mRNA-1;Parent=Msa0746090;Name=Msa0746090-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|0.5|1|1|1|2|0|139
chr5_1 exon 68089445 68089849 68089445 ID=Msa0746090-mRNA-1:exon:20301;Parent=Msa0746090-mRNA-1
chr5_1 exon 68090331 68090432 68090331 ID=Msa0746090-mRNA-1:exon:20300;Parent=Msa0746090-mRNA-1
chr5_1 five_prime_UTR 68090346 68090432 68090346 ID=Msa0746090-mRNA-1:five_prime_utr;Parent=Msa0746090-mRNA-1
chr5_1 CDS 68090331 68090345 68090331 ID=Msa0746090-mRNA-1:cds;Parent=Msa0746090-mRNA-1
chr5_1 CDS 68089445 68089849 68089445 ID=Msa0746090-mRNA-1:cds;Parent=Msa0746090-mRNA-1
Gene Sequence

>Msa0746090

ATGGCCACTGTTGAGGTTGAAACACAGCAAATACCAACAACTGTGACAGAAAATGAAACAGTTGAGGTAACCAAGGTCGAGGAAACAACACCGGCCTCCGAGGTTCCCACTTCAGAACCAGCCACCACCGAAGAAGTAGTAACAGTGGAGAAAGGAGAAACTGAAGTTCCAGTTGAAGCTGAGAGTACACAAGTAACTGAGGAAGCCAAGGCAGAGGTAGAGAACCCGGAACCTGAAAAAGTTGAAGTGAAAGAGGAAGTATCAGCTGAAGAGGCTAAAGAAACTACTGAAACAGAACCGGCACCCGAAAAAGTCGAAGCAAAAGAGGAAGTATCAACTGAAGAGGCTAAAGAAACTACTGAAACAGAATCAGCAGCCATAGCAGCACCAGTTGAAGAAGTAACCACCACAGAAGCATAA

Protein sequence

>Msa0746090

MATVEVETQQIPTTVTENETVEVTKVEETTPASEVPTSEPATTEEVVTVEKGETEVPVEAESTQVTEEAKAEVENPEPEKVEVKEEVSAEEAKETTETEPAPEKVEAKEEVSTEEAKETTETESAAIAAPVEEVTTTEA*