AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1302540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1302540 MtrunA17_Chr4g0075751 64.417 163 10 3 1 138 1 140 2.62e-32 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACTGTTACTACTTCTT+CGG 0.326738 8_3:-65120390 None:intergenic
TTACCTCAACAGTGGCCATT+TGG 0.390008 8_3:-65119776 None:intergenic
GTGGTGGTTACTTCTTCAAC+TGG 0.408262 8_3:-65120647 None:intergenic
CGGGTTCTCTACCTCTGCCT+TGG 0.417414 8_3:-65120468 None:intergenic
TTCTTCAACTGGTGCTGCTA+TGG 0.423717 8_3:-65120636 None:intergenic
TGTGTACTCTCAGCTTCAAC+TGG 0.435451 8_3:-65120431 None:intergenic
GAAATATGAACTAGCAAGAA+AGG 0.445512 8_3:-65119730 None:intergenic
ACTACTTCTTCGGTGGTGGC+TGG 0.451704 8_3:-65120380 None:intergenic
TGTTACTACTTCTTCGGTGG+TGG 0.470019 8_3:-65120384 None:intergenic
ATTCATCTTTCCATTCCAAA+TGG 0.477645 8_3:+65119761 Msa1302540:exon
CAAGGCAGAGGTAGAGAACC+CGG 0.480116 8_3:+65120469 Msa1302540:CDS
CGGTGTTGTTTCCTCGACCT+TGG 0.487832 8_3:-65120333 None:intergenic
CACTGTTACTACTTCTTCGG+TGG 0.532727 8_3:-65120387 None:intergenic
GGTGGTGGCTGGTTCTGAAG+TGG 0.534204 8_3:-65120369 None:intergenic
AGAGGAAGTATCAGCTGAAG+AGG 0.536609 8_3:+65120514 Msa1302540:CDS
ATTCCAAATGGCCACTGTTG+AGG 0.536707 8_3:+65119773 Msa1302540:exon
AACTGAGGAAGCCAAGGCAG+AGG 0.538217 8_3:+65120457 Msa1302540:CDS
GAAGTAGTAACAGTGGAGAA+AGG 0.540973 8_3:+65120395 Msa1302540:CDS
TCAACAGTGGCCATTTGGAA+TGG 0.543349 8_3:-65119771 None:intergenic
GTGGTGGCTGGTTCTGAAGT+GGG 0.546159 8_3:-65120368 None:intergenic
GAAGTGGGAACCTCGGAGGC+CGG 0.549296 8_3:-65120353 None:intergenic
TAAAGAAACTACTGAAACAC+CGG 0.564807 8_3:+65120538 Msa1302540:CDS
AGAGGAAGTATCAACTGAAG+AGG 0.587702 8_3:+65120583 Msa1302540:CDS
TGGTTCTGAAGTGGGAACCT+CGG 0.596316 8_3:-65120360 None:intergenic
AGTTGAGGTAACCAAGGTCG+AGG 0.604857 8_3:+65120322 Msa1302540:CDS
GACAGAAAATGAAACAGTTG+AGG 0.607247 8_3:+65120307 Msa1302540:CDS
ACAAGTAACTGAGGAAGCCA+AGG 0.616520 8_3:+65120451 Msa1302540:CDS
TGAGAGTACACAAGTAACTG+AGG 0.633074 8_3:+65120442 Msa1302540:CDS
TGAAACAGTTGAGGTAACCA+AGG 0.643241 8_3:+65120316 Msa1302540:CDS
TTCTGAAGTGGGAACCTCGG+AGG 0.645606 8_3:-65120357 None:intergenic
GGAAACAACACCGGCCTCCG+AGG 0.649162 8_3:+65120343 Msa1302540:CDS
CACCGAAGAAGTAGTAACAG+TGG 0.674611 8_3:+65120388 Msa1302540:CDS
TCTTTGAATTACCTCAACAG+TGG 0.681859 8_3:-65119784 None:intergenic
CAAGGTCGAGGAAACAACAC+CGG 0.700669 8_3:+65120334 Msa1302540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATTATTGATCTTTTT+TGG - chr8_3:65120052-65120071 None:intergenic 15.0%
!!! AATTGATTATTGATCTTTTT+TGG - chr8_3:65120052-65120071 None:intergenic 15.0%
!! AAACAAAAATATTAGTCCAA+TGG + chr8_3:65119972-65119991 Msa1302540:intron 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG - chr8_3:65119991-65120010 None:intergenic 20.0%
!! GATTATGAAAAATAAACACA+GGG - chr8_3:65120030-65120049 None:intergenic 20.0%
!! AAACAAAAATATTAGTCCAA+TGG + chr8_3:65119972-65119991 Msa1302540:intron 20.0%
!!! ATTTGTTGTTTATAATCCAT+TGG - chr8_3:65119991-65120010 None:intergenic 20.0%
!! GATTATGAAAAATAAACACA+GGG - chr8_3:65120030-65120049 None:intergenic 20.0%
!!! AATATTTTTGTTTGATCCAC+TGG - chr8_3:65119965-65119984 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+AGG - chr8_3:65120031-65120050 None:intergenic 25.0%
!!! AATATTTTTGTTTGATCCAC+TGG - chr8_3:65119965-65119984 None:intergenic 25.0%
! GGATTATGAAAAATAAACAC+AGG - chr8_3:65120031-65120050 None:intergenic 25.0%
GAAATATGAACTAGCAAGAA+AGG - chr8_3:65119733-65119752 None:intergenic 30.0%
ATTCATCTTTCCATTCCAAA+TGG + chr8_3:65119761-65119780 Msa1302540:exon 30.0%
ATATGACCAAGTAACTCATA+GGG - chr8_3:65120205-65120224 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG - chr8_3:65120206-65120225 None:intergenic 30.0%
TAACAAAACATGTTGAGCAT+TGG + chr8_3:65120230-65120249 Msa1302540:intron 30.0%
!! TCTTTCACTTCAACTTTTTC+AGG - chr8_3:65120497-65120516 None:intergenic 30.0%
TGAAAAAGTTGAAGTGAAAG+AGG + chr8_3:65120496-65120515 Msa1302540:CDS 30.0%
TAAAGAAACTACTGAAACAC+CGG + chr8_3:65120538-65120557 Msa1302540:CDS 30.0%
GAAATATGAACTAGCAAGAA+AGG - chr8_3:65119733-65119752 None:intergenic 30.0%
ATTCATCTTTCCATTCCAAA+TGG + chr8_3:65119761-65119780 Msa1302540:exon 30.0%
ATATGACCAAGTAACTCATA+GGG - chr8_3:65120205-65120224 None:intergenic 30.0%
TATATGACCAAGTAACTCAT+AGG - chr8_3:65120206-65120225 None:intergenic 30.0%
TAACAAAACATGTTGAGCAT+TGG + chr8_3:65120230-65120249 Msa1302540:intron 30.0%
!! TCTTTCACTTCAACTTTTTC+AGG - chr8_3:65120497-65120516 None:intergenic 30.0%
TGAAAAAGTTGAAGTGAAAG+AGG + chr8_3:65120496-65120515 Msa1302540:CDS 30.0%
TAAAGAAACTACTGAAACAC+CGG + chr8_3:65120538-65120557 Msa1302540:CDS 30.0%
TCTTTGAATTACCTCAACAG+TGG - chr8_3:65119787-65119806 None:intergenic 35.0%
! GCATTGGTGGTTAATGAAAA+TGG + chr8_3:65120246-65120265 Msa1302540:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG + chr8_3:65120256-65120275 Msa1302540:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG - chr8_3:65120299-65120318 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG + chr8_3:65120307-65120326 Msa1302540:CDS 35.0%
!!! ACTTCAACTTTTTCAGGTTC+CGG - chr8_3:65120491-65120510 None:intergenic 35.0%
!! TCTTTTGCTTCGACTTTTTC+GGG - chr8_3:65120566-65120585 None:intergenic 35.0%
! CTCTTTTGCTTCGACTTTTT+CGG - chr8_3:65120567-65120586 None:intergenic 35.0%
TCTTTGAATTACCTCAACAG+TGG - chr8_3:65119787-65119806 None:intergenic 35.0%
! GCATTGGTGGTTAATGAAAA+TGG + chr8_3:65120246-65120265 Msa1302540:intron 35.0%
TTAATGAAAATGGATCCTGC+AGG + chr8_3:65120256-65120275 Msa1302540:intron 35.0%
! TCATTTTCTGTCACAGTTGT+TGG - chr8_3:65120299-65120318 None:intergenic 35.0%
GACAGAAAATGAAACAGTTG+AGG + chr8_3:65120307-65120326 Msa1302540:CDS 35.0%
!!! ACTTCAACTTTTTCAGGTTC+CGG - chr8_3:65120491-65120510 None:intergenic 35.0%
!! TCTTTTGCTTCGACTTTTTC+GGG - chr8_3:65120566-65120585 None:intergenic 35.0%
! CTCTTTTGCTTCGACTTTTT+CGG - chr8_3:65120567-65120586 None:intergenic 35.0%
! ATTCACACAGCATTTTCCAG+TGG + chr8_3:65119946-65119965 Msa1302540:intron 40.0%
CATGTTCCCTATGAGTTACT+TGG + chr8_3:65120196-65120215 Msa1302540:intron 40.0%
!! CAAAACATGTTGAGCATTGG+TGG + chr8_3:65120233-65120252 Msa1302540:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG + chr8_3:65120316-65120335 Msa1302540:CDS 40.0%
CTCCACTGTTACTACTTCTT+CGG - chr8_3:65120393-65120412 None:intergenic 40.0%
GAAGTAGTAACAGTGGAGAA+AGG + chr8_3:65120395-65120414 Msa1302540:CDS 40.0%
! TGAGAGTACACAAGTAACTG+AGG + chr8_3:65120442-65120461 Msa1302540:CDS 40.0%
!!! CTTCAACTTTTTCAGGTTCC+GGG - chr8_3:65120490-65120509 None:intergenic 40.0%
CGAAAAAGTCGAAGCAAAAG+AGG + chr8_3:65120565-65120584 Msa1302540:CDS 40.0%
!! AGAGGAAGTATCAACTGAAG+AGG + chr8_3:65120583-65120602 Msa1302540:CDS 40.0%
! ATTCACACAGCATTTTCCAG+TGG + chr8_3:65119946-65119965 Msa1302540:intron 40.0%
CATGTTCCCTATGAGTTACT+TGG + chr8_3:65120196-65120215 Msa1302540:intron 40.0%
!! CAAAACATGTTGAGCATTGG+TGG + chr8_3:65120233-65120252 Msa1302540:intron 40.0%
TGAAACAGTTGAGGTAACCA+AGG + chr8_3:65120316-65120335 Msa1302540:CDS 40.0%
CTCCACTGTTACTACTTCTT+CGG - chr8_3:65120393-65120412 None:intergenic 40.0%
GAAGTAGTAACAGTGGAGAA+AGG + chr8_3:65120395-65120414 Msa1302540:CDS 40.0%
! TGAGAGTACACAAGTAACTG+AGG + chr8_3:65120442-65120461 Msa1302540:CDS 40.0%
!!! CTTCAACTTTTTCAGGTTCC+GGG - chr8_3:65120490-65120509 None:intergenic 40.0%
CGAAAAAGTCGAAGCAAAAG+AGG + chr8_3:65120565-65120584 Msa1302540:CDS 40.0%
!! AGAGGAAGTATCAACTGAAG+AGG + chr8_3:65120583-65120602 Msa1302540:CDS 40.0%
TCAACAGTGGCCATTTGGAA+TGG - chr8_3:65119774-65119793 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG + chr8_3:65119773-65119792 Msa1302540:exon 45.0%
TTACCTCAACAGTGGCCATT+TGG - chr8_3:65119779-65119798 None:intergenic 45.0%
! TTTGCTGTGTTTCAACCTGC+AGG - chr8_3:65120274-65120293 None:intergenic 45.0%
TGTTACTACTTCTTCGGTGG+TGG - chr8_3:65120387-65120406 None:intergenic 45.0%
CACTGTTACTACTTCTTCGG+TGG - chr8_3:65120390-65120409 None:intergenic 45.0%
CACCGAAGAAGTAGTAACAG+TGG + chr8_3:65120388-65120407 Msa1302540:CDS 45.0%
TGTGTACTCTCAGCTTCAAC+TGG - chr8_3:65120434-65120453 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+AGG + chr8_3:65120451-65120470 Msa1302540:CDS 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG + chr8_3:65120514-65120533 Msa1302540:CDS 45.0%
!! TTCTTCAACTGGTGCTGCTA+TGG - chr8_3:65120639-65120658 None:intergenic 45.0%
GTGGTGGTTACTTCTTCAAC+TGG - chr8_3:65120650-65120669 None:intergenic 45.0%
TCAACAGTGGCCATTTGGAA+TGG - chr8_3:65119774-65119793 None:intergenic 45.0%
ATTCCAAATGGCCACTGTTG+AGG + chr8_3:65119773-65119792 Msa1302540:exon 45.0%
TTACCTCAACAGTGGCCATT+TGG - chr8_3:65119779-65119798 None:intergenic 45.0%
! TTTGCTGTGTTTCAACCTGC+AGG - chr8_3:65120274-65120293 None:intergenic 45.0%
TGTTACTACTTCTTCGGTGG+TGG - chr8_3:65120387-65120406 None:intergenic 45.0%
CACTGTTACTACTTCTTCGG+TGG - chr8_3:65120390-65120409 None:intergenic 45.0%
CACCGAAGAAGTAGTAACAG+TGG + chr8_3:65120388-65120407 Msa1302540:CDS 45.0%
TGTGTACTCTCAGCTTCAAC+TGG - chr8_3:65120434-65120453 None:intergenic 45.0%
ACAAGTAACTGAGGAAGCCA+AGG + chr8_3:65120451-65120470 Msa1302540:CDS 45.0%
!! AGAGGAAGTATCAGCTGAAG+AGG + chr8_3:65120514-65120533 Msa1302540:CDS 45.0%
!! TTCTTCAACTGGTGCTGCTA+TGG - chr8_3:65120639-65120658 None:intergenic 45.0%
GTGGTGGTTACTTCTTCAAC+TGG - chr8_3:65120650-65120669 None:intergenic 45.0%
AGTTGAGGTAACCAAGGTCG+AGG + chr8_3:65120322-65120341 Msa1302540:CDS 50.0%
CAAGGTCGAGGAAACAACAC+CGG + chr8_3:65120334-65120353 Msa1302540:CDS 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG - chr8_3:65120363-65120382 None:intergenic 50.0%
AGTTGAGGTAACCAAGGTCG+AGG + chr8_3:65120322-65120341 Msa1302540:CDS 50.0%
CAAGGTCGAGGAAACAACAC+CGG + chr8_3:65120334-65120353 Msa1302540:CDS 50.0%
!! TGGTTCTGAAGTGGGAACCT+CGG - chr8_3:65120363-65120382 None:intergenic 50.0%
!! CGGTGTTGTTTCCTCGACCT+TGG - chr8_3:65120336-65120355 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG - chr8_3:65120360-65120379 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG - chr8_3:65120371-65120390 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG - chr8_3:65120383-65120402 None:intergenic 55.0%
AACTGAGGAAGCCAAGGCAG+AGG + chr8_3:65120457-65120476 Msa1302540:CDS 55.0%
CAAGGCAGAGGTAGAGAACC+CGG + chr8_3:65120469-65120488 Msa1302540:CDS 55.0%
!!! GCTTCGACTTTTTCGGGTGC+CGG - chr8_3:65120560-65120579 None:intergenic 55.0%
!! CGGTGTTGTTTCCTCGACCT+TGG - chr8_3:65120336-65120355 None:intergenic 55.0%
!! TTCTGAAGTGGGAACCTCGG+AGG - chr8_3:65120360-65120379 None:intergenic 55.0%
!! GTGGTGGCTGGTTCTGAAGT+GGG - chr8_3:65120371-65120390 None:intergenic 55.0%
! ACTACTTCTTCGGTGGTGGC+TGG - chr8_3:65120383-65120402 None:intergenic 55.0%
AACTGAGGAAGCCAAGGCAG+AGG + chr8_3:65120457-65120476 Msa1302540:CDS 55.0%
CAAGGCAGAGGTAGAGAACC+CGG + chr8_3:65120469-65120488 Msa1302540:CDS 55.0%
!!! GCTTCGACTTTTTCGGGTGC+CGG - chr8_3:65120560-65120579 None:intergenic 55.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG - chr8_3:65120372-65120391 None:intergenic 60.0%
CGGGTTCTCTACCTCTGCCT+TGG - chr8_3:65120471-65120490 None:intergenic 60.0%
!! GGTGGTGGCTGGTTCTGAAG+TGG - chr8_3:65120372-65120391 None:intergenic 60.0%
CGGGTTCTCTACCTCTGCCT+TGG - chr8_3:65120471-65120490 None:intergenic 60.0%
GGAAACAACACCGGCCTCCG+AGG + chr8_3:65120343-65120362 Msa1302540:CDS 65.0%
GAAGTGGGAACCTCGGAGGC+CGG - chr8_3:65120356-65120375 None:intergenic 65.0%
GGAAACAACACCGGCCTCCG+AGG + chr8_3:65120343-65120362 Msa1302540:CDS 65.0%
GAAGTGGGAACCTCGGAGGC+CGG - chr8_3:65120356-65120375 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 65119693 65120679 65119693 ID=Msa1302540;Name=Msa1302540
chr8_3 mRNA 65119693 65120679 65119693 ID=Msa1302540-mRNA-1;Parent=Msa1302540;Name=Msa1302540-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|0.5|1|1|1|2|0|138
chr8_3 exon 65119693 65119794 65119693 ID=Msa1302540-mRNA-1:exon:19719;Parent=Msa1302540-mRNA-1
chr8_3 exon 65120278 65120679 65120278 ID=Msa1302540-mRNA-1:exon:19720;Parent=Msa1302540-mRNA-1
chr8_3 five_prime_UTR 65119693 65119779 65119693 ID=Msa1302540-mRNA-1:five_prime_utr;Parent=Msa1302540-mRNA-1
chr8_3 CDS 65119780 65119794 65119780 ID=Msa1302540-mRNA-1:cds;Parent=Msa1302540-mRNA-1
chr8_3 CDS 65120278 65120679 65120278 ID=Msa1302540-mRNA-1:cds;Parent=Msa1302540-mRNA-1
Gene Sequence

>Msa1302540

ATGGCCACTGTTGAGGTTGAAACACAGCAAATACCAACAACTGTGACAGAAAATGAAACAGTTGAGGTAACCAAGGTCGAGGAAACAACACCGGCCTCCGAGGTTCCCACTTCAGAACCAGCCACCACCGAAGAAGTAGTAACAGTGGAGAAAGGAGAAACTGAAGTTCCAGTTGAAGCTGAGAGTACACAAGTAACTGAGGAAGCCAAGGCAGAGGTAGAGAACCCGGAACCTGAAAAAGTTGAAGTGAAAGAGGAAGTATCAGCTGAAGAGGCTAAAGAAACTACTGAAACACCGGCACCCGAAAAAGTCGAAGCAAAAGAGGAAGTATCAACTGAAGAGGCTAAAGAAACTACTGAAACAGAATCAGCAGCCATAGCAGCACCAGTTGAAGAAGTAACCACCACAGAAGCATAA

Protein sequence

>Msa1302540

MATVEVETQQIPTTVTENETVEVTKVEETTPASEVPTSEPATTEEVVTVEKGETEVPVEAESTQVTEEAKAEVENPEPEKVEVKEEVSAEEAKETTETPAPEKVEAKEEVSTEEAKETTETESAAIAAPVEEVTTTEA*