Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1349870 | XP_003630917.1 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 3.45e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1349870 | B7FHH4 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 1.65e-89 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029730 | Msa1349870 | 0.823842 | 1.113901e-53 | -8.615850e-47 |
| Msa1349870 | Msa1394810 | -0.805272 | 1.446488e-49 | -8.615850e-47 |
| Msa0662670 | Msa1349870 | -0.802018 | 6.844225e-49 | -8.615850e-47 |
| Msa0706130 | Msa1349870 | -0.803142 | 4.014657e-49 | -8.615850e-47 |
| Msa0708870 | Msa1349870 | -0.805425 | 1.343129e-49 | -8.615850e-47 |
| Msa0812330 | Msa1349870 | 0.808280 | 3.346230e-50 | -8.615850e-47 |
| Msa0195030 | Msa1349870 | 0.803865 | 2.842971e-49 | -8.615850e-47 |
| Msa1225040 | Msa1349870 | 0.811060 | 8.455702e-51 | -8.615850e-47 |
| Msa1279330 | Msa1349870 | -0.832988 | 6.901276e-56 | -8.615850e-47 |
| Msa1307730 | Msa1349870 | 0.812792 | 3.547574e-51 | -8.615850e-47 |
| Msa1326800 | Msa1349870 | -0.801627 | 8.237107e-49 | -8.615850e-47 |
| Msa0389800 | Msa1349870 | 0.815458 | 9.149977e-52 | -8.615850e-47 |
| Msa0982260 | Msa1349870 | 0.817590 | 3.047000e-52 | -8.615850e-47 |
| Msa1035640 | Msa1349870 | -0.801164 | 1.024293e-48 | -8.615850e-47 |
| Msa0274940 | Msa1349870 | 0.809709 | 1.655102e-50 | -8.615850e-47 |
| Msa0309670 | Msa1349870 | -0.810799 | 9.631975e-51 | -8.615850e-47 |
| Msa0315660 | Msa1349870 | 0.806929 | 6.478662e-50 | -8.615850e-47 |
| Msa0349220 | Msa1349870 | -0.819491 | 1.129455e-52 | -8.615850e-47 |
| Msa1128230 | Msa1349870 | -0.805861 | 1.088049e-49 | -8.615850e-47 |
| Msa1199670 | Msa1349870 | -0.810962 | 8.880381e-51 | -8.615850e-47 |
| Msa0528040 | Msa1349870 | 0.809560 | 1.781684e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1349870 | MtrunA17_Chr8g0391491 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 3.17e-93 | 265 |
| Msa1349870 | MtrunA17_Chr6g0479251 | 40.157 | 127 | 76 | 0 | 1 | 127 | 1 | 127 | 1.60e-29 | 103 |
| Msa1349870 | MtrunA17_Chr5g0420491 | 40.157 | 127 | 76 | 0 | 1 | 127 | 1 | 127 | 2.29e-29 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1349870 | AT5G17190.1 | 88.462 | 130 | 15 | 0 | 1 | 130 | 1 | 130 | 1.12e-85 | 246 |
| Msa1349870 | AT3G03160.1 | 86.154 | 130 | 18 | 0 | 1 | 130 | 1 | 130 | 4.73e-83 | 239 |
| Msa1349870 | AT1G48440.1 | 44.615 | 130 | 65 | 1 | 1 | 130 | 1 | 123 | 3.09e-32 | 110 |
| Msa1349870 | AT3G17780.1 | 41.538 | 130 | 69 | 1 | 1 | 130 | 1 | 123 | 6.68e-26 | 94.7 |
Find 35 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCTGATGGACATTTAT+TGG | 0.134696 | tig0011086:+46273 | Msa1349870:CDS |
| CCGTTACCAATCTTGTTGTT+AGG | 0.304921 | tig0011086:+46435 | Msa1349870:CDS |
| AAACGGTTTGAGAAGATTCT+TGG | 0.339054 | tig0011086:-46225 | None:intergenic |
| CGGTCCTCTTGAGGCGTTCA+AGG | 0.371336 | tig0011086:-46478 | None:intergenic |
| CTGCTGCGCTTCTCTTCTAC+TGG | 0.392268 | tig0011086:+46402 | Msa1349870:CDS |
| CGCAGCAGCAGAAGCGATCA+TGG | 0.402746 | tig0011086:+46145 | Msa1349870:CDS |
| TTGCGAAGAGCGTCGAGGCC+GGG | 0.422996 | tig0011086:-46185 | None:intergenic |
| ACGAAATTTGGCAATTCATT+CGG | 0.424763 | tig0011086:-46498 | None:intergenic |
| ATCACCTTCACAGCTTGGCC+TGG | 0.445526 | tig0011086:-46306 | None:intergenic |
| TGCGAAGAGCGTCGAGGCCG+GGG | 0.453391 | tig0011086:-46184 | None:intergenic |
| CGGCCTCGACGCTCTTCGCA+AGG | 0.457866 | tig0011086:+46187 | Msa1349870:CDS |
| ATCGATCCTAACAACAAGAT+TGG | 0.462174 | tig0011086:-46441 | None:intergenic |
| CTGAATCAATGGCGTTGGAA+TGG | 0.464316 | tig0011086:+46105 | None:intergenic |
| TCTTGAGGCGTTCAAGGCGC+TGG | 0.467572 | tig0011086:-46472 | None:intergenic |
| CAAGAATCACCTTCACAGCT+TGG | 0.469024 | tig0011086:-46311 | None:intergenic |
| CAATTCATTCGGTCCTCTTG+AGG | 0.472139 | tig0011086:-46487 | None:intergenic |
| CTTGCGAAGAGCGTCGAGGC+CGG | 0.486698 | tig0011086:-46186 | None:intergenic |
| TGAATCAATGGCGTTGGAAT+GGG | 0.503262 | tig0011086:+46106 | None:intergenic |
| AACGGAACCACCGAGAGAAA+CGG | 0.508904 | tig0011086:-46242 | None:intergenic |
| ATCAGAAGAAAGAGGCAAAA+CGG | 0.519304 | tig0011086:-46260 | None:intergenic |
| TCTTCTCAAACCGTTTCTCT+CGG | 0.531649 | tig0011086:+46232 | Msa1349870:CDS |
| AGCGCCTTGAACGCCTCAAG+AGG | 0.535773 | tig0011086:+46474 | Msa1349870:CDS |
| CATGATCGCTTCTGCTGCTG+CGG | 0.545061 | tig0011086:-46144 | None:intergenic |
| AAATGTCCATCAGAAGAAAG+AGG | 0.548010 | tig0011086:-46268 | None:intergenic |
| TCTCAAACCGTTTCTCTCGG+TGG | 0.553740 | tig0011086:+46235 | Msa1349870:CDS |
| AGGTGATTCTTGCACTCCAT+CGG | 0.561627 | tig0011086:+46322 | Msa1349870:CDS |
| GGCCTCGACGCTCTTCGCAA+GGG | 0.573478 | tig0011086:+46188 | Msa1349870:CDS |
| GAAACCAGGCCAAGCTGTGA+AGG | 0.575504 | tig0011086:+46302 | Msa1349870:CDS |
| GAGATCTGAATCAATGGCGT+TGG | 0.578135 | tig0011086:+46100 | None:intergenic |
| TGATGACGAAGATGTTCCGA+TGG | 0.580742 | tig0011086:-46338 | None:intergenic |
| GCGTTGGAATGGGTTGTGCT+AGG | 0.592663 | tig0011086:+46116 | Msa1349870:CDS |
| TTTATTGGAAATACGAAACC+AGG | 0.610945 | tig0011086:+46288 | Msa1349870:CDS |
| GTGCTTCTTCTCACAATCCC+CGG | 0.623193 | tig0011086:+46167 | Msa1349870:CDS |
| CCTAACAACAAGATTGGTAA+CGG | 0.630289 | tig0011086:-46435 | None:intergenic |
| GTCCCTTGCGAAGAGCGTCG+AGG | 0.706877 | tig0011086:-46190 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTCTGATGGACATTTAT+TGG | + | tig0011086:46273-46292 | Msa1349870:CDS | 30.0% |
| TTTATTGGAAATACGAAACC+AGG | + | tig0011086:46288-46307 | Msa1349870:CDS | 30.0% | |
| AAACGGTTTGAGAAGATTCT+TGG | - | tig0011086:46228-46247 | None:intergenic | 35.0% | |
| ATCAGAAGAAAGAGGCAAAA+CGG | - | tig0011086:46263-46282 | None:intergenic | 35.0% | |
| AAATGTCCATCAGAAGAAAG+AGG | - | tig0011086:46271-46290 | None:intergenic | 35.0% | |
| CCTAACAACAAGATTGGTAA+CGG | - | tig0011086:46438-46457 | None:intergenic | 35.0% | |
| ATCGATCCTAACAACAAGAT+TGG | - | tig0011086:46444-46463 | None:intergenic | 35.0% | |
| TCTTCTCAAACCGTTTCTCT+CGG | + | tig0011086:46232-46251 | Msa1349870:CDS | 40.0% | |
| ! | GTTTTGCCTCTTTCTTCTGA+TGG | + | tig0011086:46262-46281 | Msa1349870:CDS | 40.0% |
| !! | CCGTTACCAATCTTGTTGTT+AGG | + | tig0011086:46435-46454 | Msa1349870:CDS | 40.0% |
| CAAGAATCACCTTCACAGCT+TGG | - | tig0011086:46314-46333 | None:intergenic | 45.0% | |
| AGGTGATTCTTGCACTCCAT+CGG | + | tig0011086:46322-46341 | Msa1349870:CDS | 45.0% | |
| ! | TGATGACGAAGATGTTCCGA+TGG | - | tig0011086:46341-46360 | None:intergenic | 45.0% |
| GTGCTTCTTCTCACAATCCC+CGG | + | tig0011086:46167-46186 | Msa1349870:CDS | 50.0% | |
| TCTCAAACCGTTTCTCTCGG+TGG | + | tig0011086:46235-46254 | Msa1349870:CDS | 50.0% | |
| AACGGAACCACCGAGAGAAA+CGG | - | tig0011086:46245-46264 | None:intergenic | 50.0% | |
| !! | GCGTTGGAATGGGTTGTGCT+AGG | + | tig0011086:46116-46135 | Msa1349870:CDS | 55.0% |
| CATGATCGCTTCTGCTGCTG+CGG | - | tig0011086:46147-46166 | None:intergenic | 55.0% | |
| GAAACCAGGCCAAGCTGTGA+AGG | + | tig0011086:46302-46321 | Msa1349870:CDS | 55.0% | |
| ATCACCTTCACAGCTTGGCC+TGG | - | tig0011086:46309-46328 | None:intergenic | 55.0% | |
| CTGCTGCGCTTCTCTTCTAC+TGG | + | tig0011086:46402-46421 | Msa1349870:CDS | 55.0% | |
| CGCAGCAGCAGAAGCGATCA+TGG | + | tig0011086:46145-46164 | Msa1349870:CDS | 60.0% | |
| ! | TCTTGAGGCGTTCAAGGCGC+TGG | - | tig0011086:46475-46494 | None:intergenic | 60.0% |
| AGCGCCTTGAACGCCTCAAG+AGG | + | tig0011086:46474-46493 | Msa1349870:CDS | 60.0% | |
| ! | CGGTCCTCTTGAGGCGTTCA+AGG | - | tig0011086:46481-46500 | None:intergenic | 60.0% |
| !! | TTGCGAAGAGCGTCGAGGCC+GGG | - | tig0011086:46188-46207 | None:intergenic | 65.0% |
| !! | CTTGCGAAGAGCGTCGAGGC+CGG | - | tig0011086:46189-46208 | None:intergenic | 65.0% |
| GGCCTCGACGCTCTTCGCAA+GGG | + | tig0011086:46188-46207 | Msa1349870:CDS | 65.0% | |
| !! | GTCCCTTGCGAAGAGCGTCG+AGG | - | tig0011086:46193-46212 | None:intergenic | 65.0% |
| !! | TGCGAAGAGCGTCGAGGCCG+GGG | - | tig0011086:46187-46206 | None:intergenic | 70.0% |
| CGGCCTCGACGCTCTTCGCA+AGG | + | tig0011086:46187-46206 | Msa1349870:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0011086 | gene | 46113 | 46505 | 46113 | ID=Msa1349870;Name=Msa1349870 |
| tig0011086 | mRNA | 46113 | 46505 | 46113 | ID=Msa1349870-mRNA-1;Parent=Msa1349870;Name=Msa1349870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|130 |
| tig0011086 | exon | 46113 | 46505 | 46113 | ID=Msa1349870-mRNA-1:exon:7147;Parent=Msa1349870-mRNA-1 |
| tig0011086 | CDS | 46113 | 46505 | 46113 | ID=Msa1349870-mRNA-1:cds;Parent=Msa1349870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
