Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0529840 | XP_013454516.1 | 97.155 | 457 | 13 | 0 | 1 | 457 | 1 | 457 | 0.0 | 917 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0529840 | sp|Q8W1D5|CIPKP_ARATH | 60.674 | 445 | 168 | 1 | 10 | 454 | 33 | 470 | 0.0 | 561 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0529840 | A0A072UFT8 | 97.155 | 457 | 13 | 0 | 1 | 457 | 1 | 457 | 0.0 | 917 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0115190 | Msa0529840 | 0.803862 | 2.846132e-49 | -8.615850e-47 |
| Msa0529840 | Msa0585830 | 0.848676 | 5.265910e-60 | -8.615850e-47 |
| Msa0529840 | Msa0628030 | 0.966298 | 9.816468e-126 | -8.615850e-47 |
| Msa0529840 | Msa0663490 | 0.966578 | 4.153008e-126 | -8.615850e-47 |
| Msa0529840 | Msa0795730 | 0.810302 | 1.233285e-50 | -8.615850e-47 |
| Msa0529840 | Msa0833470 | 0.807458 | 5.006626e-50 | -8.615850e-47 |
| Msa0529840 | Msa1107200 | 0.803260 | 3.794803e-49 | -8.615850e-47 |
| Msa0529840 | Msa1180500 | 0.806766 | 7.014274e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0529840 | MtrunA17_Chr4g0001331 | 97.155 | 457 | 13 | 0 | 1 | 457 | 1 | 457 | 0.0 | 917 |
| Msa0529840 | MtrunA17_Chr2g0333821 | 77.149 | 442 | 90 | 3 | 13 | 448 | 7 | 443 | 0.0 | 678 |
| Msa0529840 | MtrunA17_Chr1g0150321 | 59.497 | 437 | 156 | 2 | 14 | 449 | 7 | 423 | 0.0 | 515 |
| Msa0529840 | MtrunA17_Chr7g0244521 | 53.917 | 434 | 197 | 3 | 14 | 446 | 6 | 437 | 2.66e-166 | 476 |
| Msa0529840 | MtrunA17_Chr4g0013131 | 54.566 | 438 | 189 | 5 | 14 | 449 | 6 | 435 | 5.13e-165 | 473 |
| Msa0529840 | MtrunA17_Chr5g0432471 | 48.918 | 462 | 209 | 6 | 10 | 449 | 2 | 458 | 4.00e-152 | 441 |
| Msa0529840 | MtrunA17_Chr3g0107011 | 50.572 | 437 | 206 | 7 | 14 | 446 | 8 | 438 | 9.95e-146 | 424 |
| Msa0529840 | MtrunA17_Chr2g0284031 | 49.558 | 452 | 196 | 9 | 14 | 443 | 25 | 466 | 1.22e-143 | 421 |
| Msa0529840 | MtrunA17_Chr2g0284011 | 48.984 | 443 | 218 | 5 | 10 | 448 | 2 | 440 | 6.87e-143 | 416 |
| Msa0529840 | MtrunA17_Chr8g0379091 | 47.708 | 480 | 218 | 12 | 2 | 456 | 11 | 482 | 9.12e-142 | 416 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0529840 | AT5G25110.1 | 60.674 | 445 | 168 | 1 | 10 | 454 | 33 | 470 | 0.0 | 561 |
| Msa0529840 | AT5G10930.1 | 59.910 | 444 | 171 | 3 | 13 | 455 | 5 | 442 | 0.0 | 548 |
| Msa0529840 | AT2G25090.1 | 54.292 | 466 | 186 | 8 | 1 | 454 | 1 | 451 | 1.26e-170 | 488 |
| Msa0529840 | AT5G45820.1 | 54.483 | 435 | 181 | 3 | 15 | 448 | 7 | 425 | 1.37e-166 | 476 |
| Msa0529840 | AT5G58380.1 | 49.672 | 457 | 207 | 5 | 9 | 449 | 1 | 450 | 2.76e-154 | 447 |
| Msa0529840 | AT4G18700.1 | 50.441 | 454 | 198 | 8 | 7 | 443 | 13 | 456 | 9.59e-149 | 433 |
| Msa0529840 | AT5G01810.1 | 51.724 | 435 | 190 | 5 | 14 | 447 | 6 | 421 | 1.50e-146 | 425 |
| Msa0529840 | AT5G01810.2 | 51.724 | 435 | 190 | 5 | 14 | 447 | 6 | 421 | 1.50e-146 | 425 |
| Msa0529840 | AT4G30960.1 | 50.557 | 449 | 196 | 5 | 4 | 445 | 8 | 437 | 3.86e-146 | 424 |
| Msa0529840 | AT1G30270.1 | 49.884 | 431 | 203 | 8 | 19 | 444 | 30 | 452 | 2.82e-143 | 419 |
Find 99 sgRNAs with CRISPR-Local
Find 242 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTATGGCAACAAA+GGG | 0.532839 | 4_1:+3614276 | Msa0529840:CDS |
| AAAGCTGATTTATGGTCTTG+TGG | 0.435090 | 4_1:+3614624 | Msa0529840:CDS |
| AAATGAGAGTGTAGCCATCA+AGG | 0.545380 | 4_1:+3614152 | Msa0529840:CDS |
| AACAATGTTGGAGAAACTTG+AGG | 0.560233 | 4_1:+3615109 | Msa0529840:CDS |
| AACAGGGAAGAAAGAGTTTG+TGG | 0.473437 | 4_1:+3615160 | Msa0529840:CDS |
| AACTCAACCACTGCCACCTC+TGG | 0.414624 | 4_1:-3615254 | None:intergenic |
| AACTTTGATATAAACATTGA+TGG | 0.368033 | 4_1:-3615077 | None:intergenic |
| AAGCTGATTTATGGTCTTGT+GGG | 0.436886 | 4_1:+3614625 | Msa0529840:CDS |
| AAGTTAAATTTGAAGATAAC+AGG | 0.292267 | 4_1:+3615143 | Msa0529840:CDS |
| AATATTCAACAAATATGAGA+TGG | 0.510172 | 4_1:+3614071 | Msa0529840:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTTGAACTCAAAGAAGTTA+TGG | + | chr4_1:3614266-3614285 | Msa0529840:CDS | 30.0% | |
| AAAGAAGTTATGGCAACAAA+GGG | + | chr4_1:3614276-3614295 | Msa0529840:CDS | 30.0% | |
| GGGAAAAATATTTCTTGTAG+TGG | + | chr4_1:3614296-3614315 | Msa0529840:CDS | 30.0% | |
| TTGAAGAAGAAAGGTTATGA+TGG | + | chr4_1:3614597-3614616 | Msa0529840:CDS | 30.0% | |
| ATTCTCTATGCTTTACTTTG+TGG | + | chr4_1:3614651-3614670 | Msa0529840:CDS | 30.0% | |
| ATAACATTCTCACCTTGAAA+AGG | - | chr4_1:3614684-3614703 | None:intergenic | 30.0% | |
| TTCAATACTCAAATCCTTCA+TGG | - | chr4_1:3614883-3614902 | None:intergenic | 30.0% | |
| ATAATTTCATCGTTGTCACA+TGG | + | chr4_1:3615014-3615033 | Msa0529840:CDS | 30.0% | |
| CAAACAATATCTTTGAGTGA+AGG | - | chr4_1:3615338-3615357 | None:intergenic | 30.0% | |
| CTTCACTCAAAGATATTGTT+TGG | + | chr4_1:3615336-3615355 | Msa0529840:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 3614027 | 3615400 | 3614027 | ID=Msa0529840;Name=Msa0529840 |
| chr4_1 | mRNA | 3614027 | 3615400 | 3614027 | ID=Msa0529840-mRNA-1;Parent=Msa0529840;Name=Msa0529840-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|457 |
| chr4_1 | exon | 3614027 | 3615400 | 3614027 | ID=Msa0529840-mRNA-1:exon:1062;Parent=Msa0529840-mRNA-1 |
| chr4_1 | CDS | 3614027 | 3615400 | 3614027 | ID=Msa0529840-mRNA-1:cds;Parent=Msa0529840-mRNA-1 |
| Gene Sequence |
>Msa0529840 ATGGATCAACAAAACAAAAAAAGTTTCCAAAACCCAAGAAACGTAATATTCAACAAATATGAGATGGGTAAAGTCTTGGGTCAAGGAAACTTTGCAAAGGTTTACCATTGTAAAAACCTTTCAGAAAATGAGAGTGTAGCCATCAAGGTGATAAAGAAAGAGAGGTTGAAAAAAGAGAGGTTGGTGAAGCAAATCAAGCGCGAAGTTTCAGTGATGCATCTCGTTCGCCATCCTCATATTGTTGAACTCAAAGAAGTTATGGCAACAAAGGGAAAAATATTTCTTGTAGTGGAATATGTAAAAGGAGGTGAACTCTTCAACAAGTTGGCCAAAGGAAAATTGAATGAAGATGTTGCTAGAAAGTACTTTCAGCAACTCATAAGTGCCGTAGATTTTTGCCATAGTCGTGGTGTCACACATCGCGATTTGAAACCGGAAAATCTACTCTTAGATGAAAACGAAGACCTTAAGGTCTCCGATTTTGGACTCTCAGCGTTGCCAGGACAACGCCGAGAGGATGGGATGCTTTTAACTCCGTGTGGAACCCCTGCTTACGTGGCACCTGAGGTTTTGAAGAAGAAAGGTTATGATGGTTCTAAAGCTGATTTATGGTCTTGTGGGGTAATTCTCTATGCTTTACTTTGTGGATATCTTCCTTTTCAAGGTGAGAATGTTATGAGAATTTATAGAAAGACTTTTAGAGGCGAATATGAGTTTCCTGAATGGATTTCTCCACAAGCTAAAATATTGATTTCGAATCTTCTTGTTCCTGATCCTAAAAAGAGGTATTCAATTGTTGATATTATGAATGATCCTTGGTTTCGAGTTGGGTTTGTTAGACCCATTGCATTTTCCATGAAGGATTTGAGTATTGAAGATAACGACAAAGATGTTAATATTGATTTCAATGAGATTGGTGAAGATGATGTTGATATCCCTGAATTGGTAATGGTGAAACCGTCTCGTCCTTTTTATAATGCATTTGAAATAATTTCATCGTTGTCACATGGCTTTGATCTTAGGAGTTTGTTTGAAACGAGGAAGAGGTTGCCATCAATGTTTATATCAAAGTTTTCGGCTTCAACAATGTTGGAGAAACTTGAGGTGATGGCGAAGAAGTTAAATTTGAAGATAACAGGGAAGAAAGAGTTTGTGGTGAGAATGGAGGGCGTGAAGGAGGGGAGAAAGGGGAAGGTCGCGATGACAATGGAGGTATTTGAGGTGGCGCCAGAGGTGGCAGTGGTTGAGTTTTCCAAGTGTGGTGGAGACACATTGGAGTATGTTAAGATGTGTGAGGAACAATTGAGACCTTCACTCAAAGATATTGTTTGGAGTTGGCAAGGTGACAATCATAATGATAATAATCCTCAATAG |
| Protein sequence |
>Msa0529840 MDQQNKKSFQNPRNVIFNKYEMGKVLGQGNFAKVYHCKNLSENESVAIKVIKKERLKKERLVKQIKREVSVMHLVRHPHIVELKEVMATKGKIFLVVEYVKGGELFNKLAKGKLNEDVARKYFQQLISAVDFCHSRGVTHRDLKPENLLLDENEDLKVSDFGLSALPGQRREDGMLLTPCGTPAYVAPEVLKKKGYDGSKADLWSCGVILYALLCGYLPFQGENVMRIYRKTFRGEYEFPEWISPQAKILISNLLVPDPKKRYSIVDIMNDPWFRVGFVRPIAFSMKDLSIEDNDKDVNIDFNEIGEDDVDIPELVMVKPSRPFYNAFEIISSLSHGFDLRSLFETRKRLPSMFISKFSASTMLEKLEVMAKKLNLKITGKKEFVVRMEGVKEGRKGKVAMTMEVFEVAPEVAVVEFSKCGGDTLEYVKMCEEQLRPSLKDIVWSWQGDNHNDNNPQ* |
