Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0663490 | XP_013454516.1 | 97.593 | 457 | 11 | 0 | 1 | 457 | 1 | 457 | 0.0 | 922 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0663490 | sp|Q8W1D5|CIPKP_ARATH | 60.899 | 445 | 167 | 1 | 10 | 454 | 33 | 470 | 0.0 | 564 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0663490 | A0A072UFT8 | 97.593 | 457 | 11 | 0 | 1 | 457 | 1 | 457 | 0.0 | 922 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015930 | Msa0663490 | 0.808608 | 2.848537e-50 | -8.615850e-47 |
| Msa0115190 | Msa0663490 | 0.811641 | 6.324377e-51 | -8.615850e-47 |
| Msa0140630 | Msa0663490 | 0.810776 | 9.742944e-51 | -8.615850e-47 |
| Msa0156370 | Msa0663490 | 0.815817 | 7.610021e-52 | -8.615850e-47 |
| Msa0519160 | Msa0663490 | 0.806559 | 7.755415e-50 | -8.615850e-47 |
| Msa0529840 | Msa0663490 | 0.966578 | 4.153008e-126 | -8.615850e-47 |
| Msa0585830 | Msa0663490 | 0.866220 | 3.342605e-65 | -8.615850e-47 |
| Msa0628030 | Msa0663490 | 0.999049 | 1.082206e-287 | -8.615850e-47 |
| Msa0663490 | Msa0720640 | 0.802207 | 6.258243e-49 | -8.615850e-47 |
| Msa0663490 | Msa0741230 | 0.801540 | 8.581359e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0663490 | MtrunA17_Chr4g0001331 | 97.593 | 457 | 11 | 0 | 1 | 457 | 1 | 457 | 0.0 | 922 |
| Msa0663490 | MtrunA17_Chr2g0333821 | 77.149 | 442 | 90 | 3 | 13 | 448 | 7 | 443 | 0.0 | 680 |
| Msa0663490 | MtrunA17_Chr1g0150321 | 59.725 | 437 | 155 | 2 | 14 | 449 | 7 | 423 | 0.0 | 517 |
| Msa0663490 | MtrunA17_Chr7g0244521 | 54.147 | 434 | 196 | 3 | 14 | 446 | 6 | 437 | 8.97e-168 | 480 |
| Msa0663490 | MtrunA17_Chr4g0013131 | 54.795 | 438 | 188 | 5 | 14 | 449 | 6 | 435 | 1.26e-166 | 477 |
| Msa0663490 | MtrunA17_Chr5g0432471 | 48.394 | 467 | 204 | 6 | 10 | 449 | 2 | 458 | 1.19e-152 | 442 |
| Msa0663490 | MtrunA17_Chr3g0107011 | 50.801 | 437 | 205 | 7 | 14 | 446 | 8 | 438 | 1.34e-146 | 426 |
| Msa0663490 | MtrunA17_Chr2g0284031 | 49.558 | 452 | 196 | 9 | 14 | 443 | 25 | 466 | 4.68e-144 | 422 |
| Msa0663490 | MtrunA17_Chr2g0284011 | 49.210 | 443 | 217 | 5 | 10 | 448 | 2 | 440 | 8.42e-144 | 418 |
| Msa0663490 | MtrunA17_Chr8g0379091 | 47.708 | 480 | 218 | 12 | 2 | 456 | 11 | 482 | 9.12e-143 | 418 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0663490 | AT5G25110.1 | 60.899 | 445 | 167 | 1 | 10 | 454 | 33 | 470 | 0.0 | 564 |
| Msa0663490 | AT5G10930.1 | 60.135 | 444 | 170 | 3 | 13 | 455 | 5 | 442 | 0.0 | 552 |
| Msa0663490 | AT2G25090.1 | 54.506 | 466 | 185 | 9 | 1 | 454 | 1 | 451 | 1.99e-170 | 487 |
| Msa0663490 | AT5G45820.1 | 54.713 | 435 | 180 | 3 | 15 | 448 | 7 | 425 | 3.95e-168 | 480 |
| Msa0663490 | AT5G58380.1 | 49.672 | 457 | 207 | 5 | 9 | 449 | 1 | 450 | 1.97e-154 | 447 |
| Msa0663490 | AT4G18700.1 | 50.441 | 454 | 198 | 8 | 7 | 443 | 13 | 456 | 1.27e-149 | 435 |
| Msa0663490 | AT5G01810.1 | 51.724 | 435 | 190 | 5 | 14 | 447 | 6 | 421 | 1.79e-147 | 427 |
| Msa0663490 | AT5G01810.2 | 51.724 | 435 | 190 | 5 | 14 | 447 | 6 | 421 | 1.79e-147 | 427 |
| Msa0663490 | AT4G30960.1 | 50.780 | 449 | 195 | 5 | 4 | 445 | 8 | 437 | 2.00e-147 | 428 |
| Msa0663490 | AT1G30270.1 | 49.884 | 431 | 203 | 8 | 19 | 444 | 30 | 452 | 3.65e-144 | 421 |
Find 98 sgRNAs with CRISPR-Local
Find 240 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTATGGCAACAAA+GGG | 0.532839 | 4_4:-2341303 | Msa0663490:CDS |
| AAAGCTGATTTATGGTCTTG+TGG | 0.435090 | 4_4:-2340955 | Msa0663490:CDS |
| AAATGAGAGTGTAGCCATCA+AGG | 0.545380 | 4_4:-2341427 | Msa0663490:CDS |
| AACAATGTTGGAGAAACTTG+AGG | 0.560233 | 4_4:-2340470 | Msa0663490:CDS |
| AACAGGGAAGAAAGAGTTTG+TGG | 0.473437 | 4_4:-2340419 | Msa0663490:CDS |
| AACTCAACCACTGCCACCTC+TGG | 0.414624 | 4_4:+2340325 | None:intergenic |
| AACTTTGATATAAACATTGA+TGG | 0.368033 | 4_4:+2340502 | None:intergenic |
| AAGCTGATTTATGGTCTTGT+GGG | 0.436886 | 4_4:-2340954 | Msa0663490:CDS |
| AAGTTAAATTTGAAGATAAC+AGG | 0.292267 | 4_4:-2340436 | Msa0663490:CDS |
| AATATTCAACAAATATGAGA+TGG | 0.510172 | 4_4:-2341508 | Msa0663490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTTGAACTCAAAGAAGTTA+TGG | - | chr4_4:2340440-2340459 | Msa0663490:CDS | 30.0% | |
| AAAGAAGTTATGGCAACAAA+GGG | - | chr4_4:2340450-2340469 | Msa0663490:CDS | 30.0% | |
| GGGAAAAATATTTCTTGTAG+TGG | - | chr4_4:2340470-2340489 | Msa0663490:CDS | 30.0% | |
| TTGAAGAAGAAAGGTTATGA+TGG | - | chr4_4:2340771-2340790 | Msa0663490:CDS | 30.0% | |
| ATTCTCTATGCTTTACTTTG+TGG | - | chr4_4:2340825-2340844 | Msa0663490:CDS | 30.0% | |
| ATAACATTCTCACCTTGAAA+AGG | + | chr4_4:2340858-2340877 | None:intergenic | 30.0% | |
| TTCAATACTCAAATCCTTCA+TGG | + | chr4_4:2341057-2341076 | None:intergenic | 30.0% | |
| ATAATTTCATCGTTGTCACA+TGG | - | chr4_4:2341188-2341207 | Msa0663490:CDS | 30.0% | |
| CAAACAATATCTTTGAGTGA+AGG | + | chr4_4:2341512-2341531 | None:intergenic | 30.0% | |
| CTTCACTCAAAGATATTGTT+TGG | - | chr4_4:2341510-2341529 | Msa0663490:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 2340201 | 2341574 | 2340201 | ID=Msa0663490;Name=Msa0663490 |
| chr4_4 | mRNA | 2340201 | 2341574 | 2340201 | ID=Msa0663490-mRNA-1;Parent=Msa0663490;Name=Msa0663490-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|457 |
| chr4_4 | exon | 2340201 | 2341574 | 2340201 | ID=Msa0663490-mRNA-1:exon:630;Parent=Msa0663490-mRNA-1 |
| chr4_4 | CDS | 2340201 | 2341574 | 2340201 | ID=Msa0663490-mRNA-1:cds;Parent=Msa0663490-mRNA-1 |
| Gene Sequence |
>Msa0663490 ATGGATCAACAAAACAAAAAAAGTTTCCAAAACCCAAGAAACGTAATATTCAACAAATATGAGATGGGTAAAGTCTTGGGTCAAGGAAACTTTGCAAAGGTTTACCATTGTAAAAACCTTTCAGAAAATGAGAGTGTAGCCATCAAGGTGATAAAGAAAGAGAGGTTGAAAAAAGAGAGGTTGGTGAAGCAAATCAAGCGCGAAGTTTCAGTGATGCATCTCGTTCGCCATCCTCATATTGTTGAACTCAAAGAAGTTATGGCAACAAAGGGAAAAATATTTCTTGTAGTGGAATATGTAAAAGGAGGTGAACTCTTCAACAAGTTGGCCAAAGGAAAATTGAATGAAGATGTTGCTAGAAAGTACTTTCAGCAACTCATAAGTGCCGTAGATTTTTGCCATAGTCGTGGTGTCACACATCGCGATTTGAAACCGGAAAATCTACTCTTAGATGAAAACGAAGACCTTAAGGTCTCCGATTTTGGACTCTCAGCGTTGCCAGAACAACGCCGAGAGGATGGGATGCTTTTAACTCCGTGTGGAACCCCTGCTTACGTGGCACCTGAGGTTTTGAAGAAGAAAGGTTATGATGGTTCTAAAGCTGATTTATGGTCTTGTGGGGTAATTCTCTATGCTTTACTTTGTGGATATCTTCCTTTTCAAGGTGAGAATGTTATGAGAATTTATAGAAAGACTTTTAGAGGCGAATATGAGTTTCCTGAATGGATTTCTCCACAAGCTAAAATATTGATTTCGAATCTTCTTGTTCCTGATCCTAAAAAGAGGTATTCAATTGTTGATATTATGAATGATCCTTGGTTTCGAGTTGGGTTTGTTAGACCCATTGCATTTTCCATGAAGGATTTGAGTATTGAAGATAACGACAAAGATGTTAATATTGATTTCAATGAGATTGGTGAAGATGATGTTGATATCCCTGAATTGGTAATGGTGAAACCGTCTCGTCCTTTTTATAATGCATTTGAAATAATTTCATCGTTGTCACATGGCTTTGATCTTAGGAGTTTGTTTGAAACGAGGAAGAGGTTGCCATCAATGTTTATATCAAAGTTTTCGGCTTCAACAATGTTGGAGAAACTTGAGGTGATGGCGAAGAAGTTAAATTTGAAGATAACAGGGAAGAAAGAGTTTGTGGTGAGAATGGAGGGCGCGAAGGAGGGGAGAAAGGGGAAGGTCGCGATGACAATGGAGGTATTTGAGGTGGCGCCAGAGGTGGCAGTGGTTGAGTTTTCCAAGTGTGGTGGAGACACATTGGAGTATGTTAAGATGTGTGAGGAACAATTGAGACCTTCACTCAAAGATATTGTTTGGAGTTGGCAAGGTGACAATCATAATGATAATAATCCTCAATAG |
| Protein sequence |
>Msa0663490 MDQQNKKSFQNPRNVIFNKYEMGKVLGQGNFAKVYHCKNLSENESVAIKVIKKERLKKERLVKQIKREVSVMHLVRHPHIVELKEVMATKGKIFLVVEYVKGGELFNKLAKGKLNEDVARKYFQQLISAVDFCHSRGVTHRDLKPENLLLDENEDLKVSDFGLSALPEQRREDGMLLTPCGTPAYVAPEVLKKKGYDGSKADLWSCGVILYALLCGYLPFQGENVMRIYRKTFRGEYEFPEWISPQAKILISNLLVPDPKKRYSIVDIMNDPWFRVGFVRPIAFSMKDLSIEDNDKDVNIDFNEIGEDDVDIPELVMVKPSRPFYNAFEIISSLSHGFDLRSLFETRKRLPSMFISKFSASTMLEKLEVMAKKLNLKITGKKEFVVRMEGAKEGRKGKVAMTMEVFEVAPEVAVVEFSKCGGDTLEYVKMCEEQLRPSLKDIVWSWQGDNHNDNNPQ* |
