AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0538040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0538040 MtrunA17_Chr4g0001481 97.177 744 20 1 1 744 1 743 0.0 1498
Msa0538040 MtrunA17_Chr4g0073941 64.793 747 235 11 1 739 1 727 0.0 951
Msa0538040 MtrunA17_Chr1g0158861 49.732 746 326 12 14 744 5 716 0.0 715
Msa0538040 MtrunA17_Chr3g0126541 50.270 740 321 9 14 739 4 710 0.0 713
Msa0538040 MtrunA17_Chr2g0305771 52.199 682 293 6 82 744 24 691 0.0 708
Msa0538040 MtrunA17_Chr3g0139071 52.246 668 295 7 89 739 61 721 0.0 677
Msa0538040 MtrunA17_Chr2g0326871 49.517 725 327 14 20 738 10 701 0.0 677
Msa0538040 MtrunA17_Chr7g0275581 48.780 738 334 10 17 739 7 715 0.0 666
Msa0538040 MtrunA17_Chr4g0028861 55.150 602 257 7 69 666 90 682 0.0 664
Msa0538040 MtrunA17_Chr1g0181121 51.420 669 293 13 89 742 32 683 0.0 656
Msa0538040 MtrunA17_Chr7g0228181 51.156 649 294 10 100 738 43 678 0.0 655
Msa0538040 MtrunA17_Chr8g0347121 45.407 762 336 11 14 737 4 723 0.0 630
Msa0538040 MtrunA17_Chr3g0125651 47.661 684 317 12 90 743 20 692 0.0 630
Msa0538040 MtrunA17_Chr6g0481021 40.956 586 310 15 101 666 123 692 8.42e-128 396
Msa0538040 MtrunA17_Chr6g0480851 38.889 594 327 14 101 678 184 757 6.77e-121 380
Msa0538040 MtrunA17_Chr6g0480671 39.626 588 324 12 100 672 192 763 8.79e-121 380
Msa0538040 MtrunA17_Chr6g0480781 34.429 700 400 18 16 686 90 759 3.94e-117 370
Msa0538040 MtrunA17_Chr8g0360821 35.777 573 348 10 103 664 64 627 6.65e-110 348
Msa0538040 MtrunA17_Chr7g0218341 36.333 589 354 9 86 666 102 677 4.37e-109 347
Msa0538040 MtrunA17_Chr6g0480741 35.495 586 335 15 100 664 114 677 1.96e-97 316
Msa0538040 MtrunA17_Chr6g0480681 33.750 640 349 19 101 689 115 730 1.89e-91 302
Msa0538040 MtrunA17_Chr2g0318561 36.245 458 273 9 217 664 2 450 5.78e-91 293
Msa0538040 MtrunA17_Chr5g0395421 38.162 359 186 11 299 642 18 355 2.32e-60 208
Msa0538040 MtrunA17_Chr6g0480771 39.113 248 128 6 425 659 12 249 3.62e-42 155
Msa0538040 MtrunA17_Chr2g0326861 40.000 205 114 4 176 378 14 211 6.38e-40 146
Msa0538040 MtrunA17_Chr6g0480841 41.237 194 94 5 479 659 2 188 1.52e-34 131
Msa0538040 MtrunA17_Chr5g0395411 35.821 201 114 4 101 300 172 358 2.03e-32 129
Msa0538040 MtrunA17_Chr2g0326851 43.262 141 62 5 604 738 3 131 2.59e-27 107
Msa0538040 MtrunA17_Chr6g0480751 36.567 134 85 0 100 233 97 230 2.56e-23 99.4
Msa0538040 MtrunA17_Chr6g0480791 41.176 119 69 1 101 218 172 290 7.06e-22 96.7
Msa0538040 MtrunA17_Chr4g0063141 25.366 205 150 2 379 582 239 441 3.81e-17 86.3
Msa0538040 MtrunA17_Chr3g0138671 25.616 203 148 2 380 582 243 442 2.61e-16 83.2
Msa0538040 MtrunA17_Chr2g0277651 24.020 204 152 2 379 582 262 462 5.63e-15 79.0
Msa0538040 MtrunA17_Chr5g0434371 24.194 186 138 2 374 559 277 459 1.12e-12 71.6
Msa0538040 MtrunA17_Chr6g0480761 28.859 149 97 5 243 389 24 165 2.22e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0538040 AT1G19780.1 68.519 756 215 10 6 744 2 751 0.0 1024
Msa0538040 AT1G15990.1 68.133 750 209 14 6 739 2 737 0.0 999
Msa0538040 AT2G23980.9 62.914 755 253 10 1 739 1 744 0.0 955
Msa0538040 AT2G23980.10 62.914 755 253 10 1 739 1 744 0.0 955
Msa0538040 AT2G23980.1 62.914 755 253 10 1 739 1 744 0.0 955
Msa0538040 AT2G23980.4 62.914 755 253 10 1 739 1 744 0.0 955
Msa0538040 AT2G23980.5 62.914 755 253 10 1 739 1 744 0.0 955
Msa0538040 AT4G30560.1 63.431 752 239 9 1 739 1 729 0.0 949
Msa0538040 AT4G30560.4 63.431 752 239 9 1 739 1 729 0.0 949
Msa0538040 AT5G57940.1 64.441 734 238 8 11 739 1 716 0.0 943
Msa0538040 AT5G57940.2 64.441 734 238 8 11 739 1 716 0.0 943
Msa0538040 AT2G23980.3 61.054 778 253 11 1 739 1 767 0.0 941
Msa0538040 AT2G23980.2 61.054 778 253 11 1 739 1 767 0.0 941
Msa0538040 AT2G23980.8 61.054 778 253 11 1 739 1 767 0.0 941
Msa0538040 AT2G23980.7 61.054 778 253 11 1 739 1 767 0.0 941
Msa0538040 AT5G57940.3 63.896 734 235 8 11 739 1 709 0.0 930
Msa0538040 AT2G23980.6 63.049 728 246 9 24 739 1 717 0.0 928
Msa0538040 AT4G30560.5 63.636 726 231 8 24 739 1 703 0.0 924
Msa0538040 AT4G30560.2 63.636 726 231 8 24 739 1 703 0.0 924
Msa0538040 AT4G30560.3 63.636 726 231 8 24 739 1 703 0.0 924
Msa0538040 AT1G19780.2 70.425 541 145 7 217 744 2 540 0.0 754
Msa0538040 AT1G01340.2 53.707 661 279 6 92 739 59 705 0.0 718
Msa0538040 AT1G01340.1 53.707 661 279 6 92 739 54 700 0.0 717
Msa0538040 AT4G01010.1 51.489 705 293 11 53 741 24 695 0.0 704
Msa0538040 AT4G01010.2 51.489 705 293 11 53 741 24 695 0.0 704
Msa0538040 AT5G53130.1 49.730 742 326 10 14 739 4 714 0.0 692
Msa0538040 AT2G28260.1 50.946 687 305 8 55 739 21 677 0.0 683
Msa0538040 AT2G24610.1 52.444 675 287 11 89 739 60 724 0.0 682
Msa0538040 AT2G24610.2 52.444 675 287 11 89 739 75 739 0.0 681
Msa0538040 AT2G28260.2 52.024 667 289 7 74 739 1 637 0.0 680
Msa0538040 AT4G30360.1 51.672 658 299 10 99 743 69 720 0.0 655
Msa0538040 AT2G46430.1 47.241 743 327 12 14 737 5 701 0.0 653
Msa0538040 AT2G46430.2 47.241 743 327 12 14 737 5 701 0.0 653
Msa0538040 AT5G14870.1 49.552 670 303 9 98 738 37 700 0.0 647
Msa0538040 AT3G48010.1 47.640 678 303 12 98 737 41 704 0.0 623
Msa0538040 AT2G46440.1 43.339 623 309 13 99 706 28 621 4.09e-162 483
Msa0538040 AT2G46450.4 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0538040 AT2G46450.1 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0538040 AT2G46450.3 37.557 655 325 17 99 735 15 603 1.43e-140 427
Msa0538040 AT2G46450.2 37.557 655 325 17 99 735 28 616 1.93e-140 427
Msa0538040 AT2G46440.2 47.097 465 208 10 256 706 3 443 3.46e-130 394
Msa0538040 AT3G17690.1 37.563 591 338 14 90 667 147 719 4.33e-119 375
Msa0538040 AT3G17700.1 37.544 570 328 10 101 658 192 745 3.87e-115 365
Msa0538040 AT5G15410.1 36.678 578 347 9 94 658 106 677 2.88e-108 346
Msa0538040 AT5G15410.2 36.678 578 347 9 94 658 105 676 3.08e-108 346
Msa0538040 AT5G54250.2 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0538040 AT5G54250.1 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0538040 AT5G54250.4 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0538040 AT3G17690.2 37.897 504 291 10 90 589 147 632 1.03e-105 337
Msa0538040 AT3G17700.2 37.576 495 292 7 101 594 192 670 5.16e-103 331
Msa0538040 AT5G54250.3 33.854 576 360 9 100 664 78 643 4.18e-102 329
Msa0538040 AT3G17690.3 35.433 381 231 8 90 466 147 516 2.86e-66 228
Msa0538040 AT4G22200.2 24.020 204 152 2 379 582 263 463 2.29e-16 83.6
Msa0538040 AT4G22200.1 24.020 204 152 2 379 582 263 463 5.32e-16 82.8
Msa0538040 AT5G37500.1 21.702 470 273 13 99 561 55 436 8.12e-15 79.0
Msa0538040 AT5G37500.2 21.489 470 274 13 99 561 55 436 1.01e-14 79.0
Msa0538040 AT5G37500.4 20.726 468 280 12 99 561 55 436 1.32e-14 78.2
Msa0538040 AT5G37500.3 20.833 408 252 9 155 561 45 382 3.13e-14 77.0
Msa0538040 AT2G26650.1 23.902 205 153 2 379 582 239 441 3.88e-14 77.0
Msa0538040 AT2G26650.2 23.902 205 153 2 379 582 134 336 5.30e-14 76.3
Msa0538040 AT5G46240.1 22.727 198 152 1 379 576 246 442 5.62e-14 76.3
Msa0538040 AT3G02850.2 20.902 488 293 14 83 563 56 457 1.04e-13 75.5
Msa0538040 AT3G02850.1 20.902 488 293 14 83 563 56 457 1.27e-13 75.1
Msa0538040 AT3G02850.3 20.697 488 294 14 83 563 56 457 1.58e-13 74.7
Msa0538040 AT4G32650.2 23.153 203 153 2 380 582 276 475 1.58e-12 71.2
Msa0538040 AT4G32650.3 23.153 203 153 2 380 582 276 475 2.14e-12 71.2
Msa0538040 AT4G32650.1 23.153 203 153 2 380 582 276 475 2.27e-12 70.9

Find 148 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4_1:+17146501 Msa0538040:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4_1:-17147256 Msa0538050:three_prime_UTR
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4_1:+17146045 Msa0538040:CDS
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4_1:+17147559 Msa0538040:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4_1:+17144923 Msa0538040:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4_1:-17146024 Msa0538050:three_prime_UTR
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4_1:+17147534 Msa0538040:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4_1:+17147452 Msa0538040:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4_1:-17144662 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4_1:-17146837 Msa0538050:three_prime_UTR
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4_1:+17144577 Msa0538040:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4_1:-17144822 None:intergenic
ATGATGTAGGTTGGATGATT+TGG 0.281948 4_1:+17144442 Msa0538040:intron
CAGAGAAGACTTACATAGTT+AGG 0.283571 4_1:+17147083 Msa0538040:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4_1:+17146803 Msa0538040:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4_1:-17146503 Msa0538050:three_prime_UTR
GATATTTCATTATTGACTTC+TGG 0.297261 4_1:+17144999 Msa0538040:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4_1:+17147535 Msa0538040:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4_1:-17146009 Msa0538050:three_prime_UTR
CATAGACAGTAACAAAATTT+GGG 0.315147 4_1:-17146023 Msa0538050:three_prime_UTR
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4_1:+17146529 Msa0538040:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4_1:-17144901 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4_1:+17146502 Msa0538040:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4_1:-17147672 Msa0538050:three_prime_UTR
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4_1:+17145171 Msa0538040:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4_1:-17147673 Msa0538050:three_prime_UTR
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4_1:-17144482 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4_1:+17144602 Msa0538040:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4_1:+17146665 Msa0538040:CDS
GCTAGATGGCATTGACGAAT+TGG 0.374592 4_1:-17144468 None:intergenic
AATGGTACGGTTCATACCTT+TGG 0.384471 4_1:+17145243 Msa0538040:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4_1:+17147587 Msa0538040:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4_1:-17147688 Msa0538050:three_prime_UTR
ATGAACAACATCTCATCAAC+TGG 0.395916 4_1:-17147115 Msa0538050:three_prime_UTR
AAGATAAGAATCTTCTGTAT+TGG 0.406036 4_1:+17144705 Msa0538040:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4_1:-17147520 Msa0538050:three_prime_UTR
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4_1:+17147607 Msa0538040:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4_1:-17147277 Msa0538050:three_prime_UTR
TACCTCACTCATAGCTTTCA+CGG 0.425446 4_1:-17147293 Msa0538050:three_prime_UTR
TACGATTTGTGGTTGAAATA+CGG 0.428941 4_1:-17144791 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4_1:+17145021 Msa0538040:CDS
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4_1:+17144630 Msa0538040:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4_1:-17147402 Msa0538050:three_prime_UTR
GTGCCATGTACTATTTGATC+TGG 0.449942 4_1:+17145317 Msa0538040:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4_1:-17146779 Msa0538050:three_prime_UTR
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4_1:-17146484 Msa0538050:three_prime_UTR
TGGCTAAACAATACGATTTG+TGG 0.457799 4_1:-17144802 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4_1:-17145238 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4_1:+17146728 Msa0538040:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4_1:+17146736 Msa0538040:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4_1:+17144950 Msa0538040:CDS
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4_1:-17144533 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4_1:-17147646 Msa0538050:three_prime_UTR
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4_1:-17146747 Msa0538050:three_prime_UTR
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4_1:-17146752 Msa0538050:three_prime_UTR
TTCTTCACAATGTTTGACTC+TGG 0.491771 4_1:+17143992 None:intergenic
AGAGAAGACTTACATAGTTA+GGG 0.493349 4_1:+17147084 Msa0538040:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4_1:-17144968 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4_1:+17145787 Msa0538040:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4_1:+17145812 Msa0538040:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4_1:+17147009 Msa0538040:intron
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4_1:+17147318 Msa0538040:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4_1:+17145840 Msa0538040:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4_1:+17146871 Msa0538040:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4_1:+17144631 Msa0538040:CDS
TACAGAAGATTCTTATCTTG+AGG 0.521699 4_1:-17144701 None:intergenic
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4_1:-17144915 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4_1:+17147410 Msa0538040:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4_1:-17146662 Msa0538050:three_prime_UTR
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4_1:-17145031 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4_1:+17146685 Msa0538040:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4_1:+17147401 Msa0538040:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4_1:+17145162 Msa0538040:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4_1:+17147231 Msa0538040:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4_1:+17147528 Msa0538040:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4_1:+17144929 Msa0538040:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4_1:+17146442 Msa0538040:intron
TACAAGTCACAGTATATTGA+AGG 0.537328 4_1:+17144016 Msa0538040:CDS
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4_1:+17147297 Msa0538040:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4_1:+17147124 Msa0538040:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4_1:+17147151 Msa0538040:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4_1:+17145864 Msa0538040:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4_1:-17147070 Msa0538050:three_prime_UTR
TAGACACATACATAGTAGAC+AGG 0.544177 4_1:+17147366 Msa0538040:CDS
ACAGTATATTGAAGGTCAGA+AGG 0.547235 4_1:+17144024 Msa0538040:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4_1:+17146668 Msa0538040:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4_1:+17146872 Msa0538040:CDS
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4_1:-17147679 Msa0538050:three_prime_UTR
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4_1:+17147178 Msa0538040:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4_1:+17146737 Msa0538040:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4_1:+17144620 Msa0538040:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4_1:-17147680 Msa0538050:three_prime_UTR
TTTGACGAACTTTGAGGACC+CGG 0.557037 4_1:-17147553 Msa0538050:three_prime_UTR
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4_1:+17145813 Msa0538040:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4_1:-17147519 Msa0538050:three_prime_UTR
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4_1:+17146676 Msa0538040:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4_1:+17147642 Msa0538040:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4_1:-17144926 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4_1:+17146540 Msa0538040:CDS
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4_1:+17147163 Msa0538040:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4_1:+17145137 Msa0538040:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4_1:+17146443 Msa0538040:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4_1:+17144476 Msa0538040:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4_1:+17147468 Msa0538040:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4_1:+17146503 Msa0538040:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4_1:+17144632 Msa0538040:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4_1:+17147464 Msa0538040:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4_1:+17147437 Msa0538040:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4_1:+17147458 Msa0538040:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4_1:+17147501 Msa0538040:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4_1:-17146715 Msa0538050:three_prime_UTR
ATATACCAGGGTATGACAGT+TGG 0.606713 4_1:+17145877 Msa0538040:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4_1:+17145134 Msa0538040:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4_1:+17146471 Msa0538040:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4_1:+17147154 Msa0538040:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4_1:+17146656 Msa0538040:intron
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4_1:+17147471 Msa0538040:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4_1:-17146744 Msa0538050:three_prime_UTR
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4_1:+17146703 Msa0538040:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4_1:-17146479 Msa0538050:three_prime_UTR
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4_1:+17147224 Msa0538040:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4_1:+17147521 Msa0538040:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4_1:+17147088 Msa0538040:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4_1:+17146783 Msa0538040:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4_1:+17146769 Msa0538040:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4_1:-17146483 Msa0538050:three_prime_UTR
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4_1:+17144566 Msa0538040:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4_1:-17147013 Msa0538050:three_prime_UTR
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4_1:+17147522 Msa0538040:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4_1:-17145320 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4_1:-17145882 Msa0538050:three_prime_UTR
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4_1:+17144477 Msa0538040:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4_1:-17146022 Msa0538050:three_prime_UTR
GTCAGAAGGAGAAATTTGTG+AGG 0.668583 4_1:+17144038 Msa0538040:CDS
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4_1:+17145751 Msa0538040:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4_1:+17146466 Msa0538040:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4_1:-17144912 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4_1:+17146784 Msa0538040:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4_1:+17147291 Msa0538040:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4_1:+17147411 Msa0538040:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4_1:+17147225 Msa0538040:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4_1:-17144907 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4_1:+17147502 Msa0538040:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4_1:-17146658 Msa0538050:three_prime_UTR
TCTATATTCAAGCTGCATGG+AGG 0.707083 4_1:+17147440 Msa0538040:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4_1:+17145165 Msa0538040:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4_1:+17145865 Msa0538040:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4_1:+17146785 Msa0538040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTATTTTGTAGATAA+CGG + chr4_1:17145740-17145759 Msa0538040:intron 15.0%
!! AAATATATATGTATTAGTGT+CGG + chr4_1:17146168-17146187 Msa0538040:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG - chr4_1:17146339-17146358 Msa0538050:intron 15.0%
!!! TTGTGAGGTATTTTTATTTT+TGG + chr4_1:17144053-17144072 Msa0538040:intron 20.0%
!!! TGTGAGGTATTTTTATTTTT+GGG + chr4_1:17144054-17144073 Msa0538040:intron 20.0%
!!! TATTGGTTAATTTAAGAACA+TGG + chr4_1:17144306-17144325 Msa0538040:intron 20.0%
!!! TTATATTCCACTTTAAGTTT+AGG + chr4_1:17144365-17144384 Msa0538040:intron 20.0%
!!! TATATTCCACTTTAAGTTTA+GGG + chr4_1:17144366-17144385 Msa0538040:intron 20.0%
!!! ACTATTATTTTGTAGATAAC+GGG + chr4_1:17145741-17145760 Msa0538040:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG - chr4_1:17146131-17146150 Msa0538050:intron 20.0%
!! GAATCAAAGCATATTTATAA+AGG - chr4_1:17146349-17146368 Msa0538050:intron 20.0%
!! ATGTTTACAGTAAAAACTTT+CGG - chr4_1:17146570-17146589 Msa0538050:intron 20.0%
! GTATAACATATCAATGTGAT+TGG + chr4_1:17144076-17144095 Msa0538040:intron 25.0%
! CTTCAAAAAAAAAAACCACT+TGG + chr4_1:17144280-17144299 Msa0538040:intron 25.0%
! AGATGCTTATGTTAACAAAT+TGG - chr4_1:17144402-17144421 None:intergenic 25.0%
! AAGATAAGAATCTTCTGTAT+TGG + chr4_1:17144705-17144724 Msa0538040:CDS 25.0%
!!! GATATTTCATTATTGACTTC+TGG + chr4_1:17144999-17145018 Msa0538040:CDS 25.0%
! CAACAAACATTAAGACATAT+TGG - chr4_1:17145122-17145141 None:intergenic 25.0%
! TCTTAAGAAATGAACTTACA+TGG - chr4_1:17145357-17145376 None:intergenic 25.0%
! TATCATTCACATATTCTCTT+TGG + chr4_1:17145472-17145491 Msa0538040:intron 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG - chr4_1:17145516-17145535 None:intergenic 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG + chr4_1:17145516-17145535 Msa0538040:intron 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG - chr4_1:17145548-17145567 None:intergenic 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG - chr4_1:17145549-17145568 None:intergenic 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG - chr4_1:17145589-17145608 None:intergenic 25.0%
! AATTCACATTTCTTGTATTG+TGG + chr4_1:17145840-17145859 Msa0538040:CDS 25.0%
! ATAGACAGTAACAAAATTTG+GGG - chr4_1:17146025-17146044 Msa0538050:three_prime_UTR 25.0%
! CATAGACAGTAACAAAATTT+GGG - chr4_1:17146026-17146045 Msa0538050:three_prime_UTR 25.0%
!!! GTTTTATAGGTGTTATAATG+AGG - chr4_1:17146118-17146137 Msa0538050:intron 25.0%
! TTCCATAGTAATAGCTATTA+TGG + chr4_1:17146501-17146520 Msa0538040:CDS 25.0%
! TCCATAGTAATAGCTATTAT+GGG + chr4_1:17146502-17146521 Msa0538040:CDS 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG - chr4_1:17146840-17146859 Msa0538050:three_prime_UTR 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG + chr4_1:17146871-17146890 Msa0538040:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG + chr4_1:17146872-17146891 Msa0538040:CDS 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG - chr4_1:17146958-17146977 Msa0538050:intron 25.0%
! GTGTATGACATATATTATTC+AGG + chr4_1:17146988-17147007 Msa0538040:intron 25.0%
TACAAGTCACAGTATATTGA+AGG + chr4_1:17144016-17144035 Msa0538040:CDS 30.0%
! GACTCTATTAATCTGGATTT+TGG + chr4_1:17144147-17144166 Msa0538040:intron 30.0%
!! TATTAATCTGGATTTTGGTC+AGG + chr4_1:17144152-17144171 Msa0538040:intron 30.0%
CTCGATAGATTTGTCATTAA+CGG + chr4_1:17144232-17144251 Msa0538040:intron 30.0%
!! AAAAAACCACTTGGTTCTAT+TGG + chr4_1:17144289-17144308 Msa0538040:intron 30.0%
AATTAACCAATAGAACCAAG+TGG - chr4_1:17144298-17144317 None:intergenic 30.0%
TCTTTCAGACTAATATACAG+TGG + chr4_1:17144419-17144438 Msa0538040:intron 30.0%
TAATATACAGTGGATGATGT+AGG + chr4_1:17144429-17144448 Msa0538040:intron 30.0%
!! TCAGATGGACTAATGAATAT+TGG + chr4_1:17144602-17144621 Msa0538040:CDS 30.0%
TACAGAAGATTCTTATCTTG+AGG - chr4_1:17144704-17144723 None:intergenic 30.0%
TACGGAAGATAGAAAAAGAA+AGG - chr4_1:17144776-17144795 None:intergenic 30.0%
TACGATTTGTGGTTGAAATA+CGG - chr4_1:17144794-17144813 None:intergenic 30.0%
! AATATTTTCCGAGAATGGTA+CGG + chr4_1:17145230-17145249 Msa0538040:CDS 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG - chr4_1:17145262-17145281 None:intergenic 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG + chr4_1:17145295-17145314 Msa0538040:CDS 30.0%
ATACCAGATCAAATAGTACA+TGG - chr4_1:17145323-17145342 None:intergenic 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG + chr4_1:17145432-17145451 Msa0538040:intron 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG - chr4_1:17145437-17145456 None:intergenic 30.0%
GGAAGTTTCCATAGAAAAAT+TGG - chr4_1:17145527-17145546 None:intergenic 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG - chr4_1:17145588-17145607 None:intergenic 30.0%
TTGTAGATATTCTGACATAG+AGG + chr4_1:17145644-17145663 Msa0538040:intron 30.0%
GTTAGAATAGATTACGCTAT+AGG + chr4_1:17145666-17145685 Msa0538040:intron 30.0%
AAAGCATGTAAAGAAGTTGA+GGG + chr4_1:17145813-17145832 Msa0538040:CDS 30.0%
!! GATAACTCAGCTTTTAACTA+TGG + chr4_1:17145948-17145967 Msa0538040:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG - chr4_1:17146012-17146031 Msa0538050:three_prime_UTR 30.0%
CCATAGACAGTAACAAAATT+TGG - chr4_1:17146027-17146046 Msa0538050:three_prime_UTR 30.0%
! CCAAATTTTGTTACTGTCTA+TGG + chr4_1:17146024-17146043 Msa0538040:CDS 30.0%
! AATTTTGTTACTGTCTATGG+TGG + chr4_1:17146027-17146046 Msa0538040:CDS 30.0%
! ATTTTGTTACTGTCTATGGT+GGG + chr4_1:17146028-17146047 Msa0538040:CDS 30.0%
ATGGCTAATGTGAAAAACAT+AGG - chr4_1:17146197-17146216 Msa0538050:intron 30.0%
TCTGATCTTCATATCAAAGT+CGG + chr4_1:17146245-17146264 Msa0538040:intron 30.0%
ATTGTAGATTCACTTGATGT+TGG + chr4_1:17146308-17146327 Msa0538040:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG + chr4_1:17146309-17146328 Msa0538040:intron 30.0%
AAGCAAAACAGAAGGATATT+TGG - chr4_1:17146423-17146442 Msa0538050:intron 30.0%
CCATAGTAATAGCTATTATG+GGG + chr4_1:17146503-17146522 Msa0538040:CDS 30.0%
CCAATTGCATTTATAACACA+AGG - chr4_1:17146633-17146652 Msa0538050:intron 30.0%
CCTTGTGTTATAAATGCAAT+TGG + chr4_1:17146630-17146649 Msa0538040:intron 30.0%
GTCTTCTCTGTATATAAACT+CGG - chr4_1:17147073-17147092 Msa0538050:three_prime_UTR 30.0%
AGAGAAGACTTACATAGTTA+GGG + chr4_1:17147084-17147103 Msa0538040:CDS 30.0%
ATATTACGAATCTGATTACC+CGG + chr4_1:17147501-17147520 Msa0538040:CDS 30.0%
ACAGTATATTGAAGGTCAGA+AGG + chr4_1:17144024-17144043 Msa0538040:CDS 35.0%
TTTCCGTTATCTTAACTCTC+TGG + chr4_1:17144108-17144127 Msa0538040:intron 35.0%
GAACCAGAGAGTTAAGATAA+CGG - chr4_1:17144114-17144133 None:intergenic 35.0%
!! TATCTTAACTCTCTGGTTCT+CGG + chr4_1:17144115-17144134 Msa0538040:intron 35.0%
GGAAAGAGACTCTATTAATC+TGG + chr4_1:17144140-17144159 Msa0538040:intron 35.0%
! AATCTATCGAGAGTATATCC+AGG - chr4_1:17144223-17144242 None:intergenic 35.0%
!! TTTTTGAAGAGAGAACCAAG+TGG - chr4_1:17144269-17144288 None:intergenic 35.0%
TGCATACCCTAAACTTAAAG+TGG - chr4_1:17144375-17144394 None:intergenic 35.0%
! ATGATGTAGGTTGGATGATT+TGG + chr4_1:17144442-17144461 Msa0538040:intron 35.0%
ATTGGAAGATCACTCAAAAG+TGG + chr4_1:17144620-17144639 Msa0538040:CDS 35.0%
!! TGTGACACTTTAAGATCTTC+TGG - chr4_1:17144665-17144684 None:intergenic 35.0%
TGGCTAAACAATACGATTTG+TGG - chr4_1:17144805-17144824 None:intergenic 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG - chr4_1:17144825-17144844 None:intergenic 35.0%
CAAAACATATTGAGCATGGA+TGG - chr4_1:17145056-17145075 None:intergenic 35.0%
TACGCAAAACATATTGAGCA+TGG - chr4_1:17145060-17145079 None:intergenic 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG + chr4_1:17145134-17145153 Msa0538040:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG + chr4_1:17145225-17145244 Msa0538040:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG + chr4_1:17145268-17145287 Msa0538040:CDS 35.0%
CATAAGTGATACAGAAGCAA+TGG - chr4_1:17145401-17145420 None:intergenic 35.0%
TCTGTATCACTTATGAAGCT+CGG + chr4_1:17145406-17145425 Msa0538040:intron 35.0%
! GAAGTATCAAGAAGTGTATG+AGG - chr4_1:17145566-17145585 None:intergenic 35.0%
AGAATAGATTACGCTATAGG+CGG + chr4_1:17145669-17145688 Msa0538040:intron 35.0%
GAAACATTGATCATTCGTGT+CGG - chr4_1:17145715-17145734 None:intergenic 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG + chr4_1:17145812-17145831 Msa0538040:CDS 35.0%
AGTTCAAGTAAGCATATACC+AGG + chr4_1:17145864-17145883 Msa0538040:CDS 35.0%
GTTCAAGTAAGCATATACCA+GGG + chr4_1:17145865-17145884 Msa0538040:CDS 35.0%
! TTTTGTTACTGTCTATGGTG+GGG + chr4_1:17146029-17146048 Msa0538040:CDS 35.0%
TGTTATAATGAGGACTCATG+TGG - chr4_1:17146108-17146127 Msa0538050:intron 35.0%
! TTTTCACATTAGCCATGTAG+AGG + chr4_1:17146201-17146220 Msa0538040:intron 35.0%
ACTGTGAAAAGCAAAACAGA+AGG - chr4_1:17146431-17146450 Msa0538050:intron 35.0%
!! TTTGCTTTTCACAGTACACT+TGG + chr4_1:17146436-17146455 Msa0538040:intron 35.0%
CCCCATAATAGCTATTACTA+TGG - chr4_1:17146506-17146525 Msa0538050:three_prime_UTR 35.0%
TGCAATGAGAAATCTCAGAA+AGG - chr4_1:17146922-17146941 Msa0538050:intron 35.0%
CAGAGAAGACTTACATAGTT+AGG + chr4_1:17147083-17147102 Msa0538040:CDS 35.0%
AAGACTTACATAGTTAGGGA+AGG + chr4_1:17147088-17147107 Msa0538040:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG - chr4_1:17147118-17147137 Msa0538050:three_prime_UTR 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG + chr4_1:17147205-17147224 Msa0538040:CDS 35.0%
TAGACACATACATAGTAGAC+AGG + chr4_1:17147366-17147385 Msa0538040:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG - chr4_1:17147405-17147424 Msa0538050:three_prime_UTR 35.0%
CAATCTATATTCAAGCTGCA+TGG + chr4_1:17147437-17147456 Msa0538040:CDS 35.0%
TATTACGAATCTGATTACCC+GGG + chr4_1:17147502-17147521 Msa0538040:CDS 35.0%
!! CCAAATTTTGACGAACTTTG+AGG - chr4_1:17147562-17147581 Msa0538050:three_prime_UTR 35.0%
CCTCAAAGTTCGTCAAAATT+TGG + chr4_1:17147559-17147578 Msa0538040:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG + chr4_1:17147587-17147606 Msa0538040:CDS 35.0%
GTCAGAAGGAGAAATTTGTG+AGG + chr4_1:17144038-17144057 Msa0538040:CDS 40.0%
!! ATCTTAACTCTCTGGTTCTC+GGG + chr4_1:17144116-17144135 Msa0538040:intron 40.0%
! ATCGAGAGTATATCCAGGTT+CGG - chr4_1:17144218-17144237 None:intergenic 40.0%
ATACAGTGGATGATGTAGGT+TGG + chr4_1:17144433-17144452 Msa0538040:intron 40.0%
! TGTTTGCCTTTTCCTATTCC+CGG + chr4_1:17144527-17144546 Msa0538040:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG - chr4_1:17144553-17144572 None:intergenic 40.0%
! TCGTCGAAATCATTTCGAGT+CGG + chr4_1:17144566-17144585 Msa0538040:CDS 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG + chr4_1:17144577-17144596 Msa0538040:CDS 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG + chr4_1:17144578-17144597 Msa0538040:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG + chr4_1:17144631-17144650 Msa0538040:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG - chr4_1:17144929-17144948 None:intergenic 40.0%
TCATCTCGAGTATTTGGAAG+AGG + chr4_1:17144929-17144948 Msa0538040:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG + chr4_1:17145137-17145156 Msa0538040:intron 40.0%
AATGGTACGGTTCATACCTT+TGG + chr4_1:17145243-17145262 Msa0538040:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG + chr4_1:17145317-17145336 Msa0538040:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG + chr4_1:17145571-17145590 Msa0538040:intron 40.0%
ATATACCAGGGTATGACAGT+TGG + chr4_1:17145877-17145896 Msa0538040:CDS 40.0%
GAAGATCAGACACACGATAT+GGG - chr4_1:17146235-17146254 Msa0538050:intron 40.0%
TGAAGATCAGACACACGATA+TGG - chr4_1:17146236-17146255 Msa0538050:intron 40.0%
! TTTTCACAGTACACTTGGTC+AGG + chr4_1:17146441-17146460 Msa0538040:intron 40.0%
TTTCACAGTACACTTGGTCA+GGG + chr4_1:17146442-17146461 Msa0538040:intron 40.0%
TGGAAAACAAAACCTCTCCA+GGG - chr4_1:17146486-17146505 Msa0538050:three_prime_UTR 40.0%
ATGGAAAACAAAACCTCTCC+AGG - chr4_1:17146487-17146506 Msa0538050:three_prime_UTR 40.0%
TCTTCTGATTGGAAACATGC+AGG + chr4_1:17146540-17146559 Msa0538040:CDS 40.0%
GACCTACCTTCAATCAATGT+CGG + chr4_1:17146656-17146675 Msa0538040:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG - chr4_1:17146661-17146680 Msa0538050:three_prime_UTR 40.0%
TGGAGGAAATGAGGATCAAA+AGG + chr4_1:17146685-17146704 Msa0538040:CDS 40.0%
! AATTTTCTTCATCGACCCCT+CGG - chr4_1:17146803-17146822 Msa0538050:three_prime_UTR 40.0%
AGGGGTCGATGAAGAAAATT+TGG + chr4_1:17146803-17146822 Msa0538040:CDS 40.0%
GAGATGTTGTTCATCATTCG+TGG + chr4_1:17147124-17147143 Msa0538040:CDS 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG + chr4_1:17147151-17147170 Msa0538040:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG + chr4_1:17147189-17147208 Msa0538040:CDS 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG + chr4_1:17147193-17147212 Msa0538040:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG + chr4_1:17147225-17147244 Msa0538040:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG - chr4_1:17147280-17147299 Msa0538050:three_prime_UTR 40.0%
TACCTCACTCATAGCTTTCA+CGG - chr4_1:17147296-17147315 Msa0538050:three_prime_UTR 40.0%
TCCGTTTCTATTCGCAACAA+TGG + chr4_1:17147401-17147420 Msa0538040:CDS 40.0%
ATTCGCAACAATGGAGAACA+TGG + chr4_1:17147410-17147429 Msa0538040:CDS 40.0%
TTCGCAACAATGGAGAACAT+GGG + chr4_1:17147411-17147430 Msa0538040:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG + chr4_1:17147440-17147459 Msa0538040:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG - chr4_1:17147691-17147710 Msa0538050:three_prime_UTR 40.0%
!! TCTTAACTCTCTGGTTCTCG+GGG + chr4_1:17144117-17144136 Msa0538040:intron 45.0%
ACGGAAGTTCATCAACCACT+TGG + chr4_1:17144251-17144270 Msa0538040:intron 45.0%
!! GCTAGATGGCATTGACGAAT+TGG - chr4_1:17144471-17144490 None:intergenic 45.0%
! TCAATGCCATCTAGCTCAAG+TGG + chr4_1:17144476-17144495 Msa0538040:CDS 45.0%
! CAATGCCATCTAGCTCAAGT+GGG + chr4_1:17144477-17144496 Msa0538040:CDS 45.0%
TTCATCCCACTTGAGCTAGA+TGG - chr4_1:17144485-17144504 None:intergenic 45.0%
GGAGTGAAAAAGGGATCAGA+TGG + chr4_1:17144587-17144606 Msa0538040:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG + chr4_1:17144630-17144649 Msa0538040:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG + chr4_1:17144632-17144651 Msa0538040:CDS 45.0%
! AGATGAAGGTGCAATGAAGG+CGG - chr4_1:17144915-17144934 None:intergenic 45.0%
! TCGAGATGAAGGTGCAATGA+AGG - chr4_1:17144918-17144937 None:intergenic 45.0%
GCACCTTCATCTCGAGTATT+TGG + chr4_1:17144923-17144942 Msa0538040:CDS 45.0%
GGTGAACTTGTCATAGATCC+TGG + chr4_1:17144950-17144969 Msa0538040:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG - chr4_1:17144971-17144990 None:intergenic 45.0%
TGGATGGTATCTCAAACCTG+TGG - chr4_1:17145040-17145059 None:intergenic 45.0%
TCTTTATAGCGCAAGACGCA+TGG + chr4_1:17145162-17145181 Msa0538040:CDS 45.0%
GGTATGAACCGTACCATTCT+CGG - chr4_1:17145241-17145260 None:intergenic 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG + chr4_1:17145751-17145770 Msa0538040:intron 45.0%
TAGAACGTAACGACACATGC+TGG + chr4_1:17145787-17145806 Msa0538040:CDS 45.0%
TTTCTCCAACTGTCATACCC+TGG - chr4_1:17145885-17145904 Msa0538050:three_prime_UTR 45.0%
GGTGGGGACTTCAGAATTTA+AGG + chr4_1:17146045-17146064 Msa0538040:CDS 45.0%
TTCACAGTACACTTGGTCAG+GGG + chr4_1:17146443-17146462 Msa0538040:intron 45.0%
AAACAAAACCTCTCCAGGGT+AGG - chr4_1:17146482-17146501 Msa0538050:three_prime_UTR 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG + chr4_1:17146529-17146548 Msa0538040:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG - chr4_1:17146665-17146684 Msa0538050:three_prime_UTR 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG + chr4_1:17146665-17146684 Msa0538040:CDS 45.0%
GGGTGTTCAACCACTTGTAT+TGG - chr4_1:17146782-17146801 Msa0538050:three_prime_UTR 45.0%
! ATTTTCTTCATCGACCCCTC+GGG - chr4_1:17146802-17146821 Msa0538050:three_prime_UTR 45.0%
GGTTCCTCTCTTTGCTAACA+TGG + chr4_1:17147009-17147028 Msa0538040:intron 45.0%
TCGTCCATGTTAGCAAAGAG+AGG - chr4_1:17147016-17147035 Msa0538050:three_prime_UTR 45.0%
!! GAGAGTGTTACTACAGATGG+TGG + chr4_1:17147154-17147173 Msa0538040:CDS 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG + chr4_1:17147163-17147182 Msa0538040:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG + chr4_1:17147178-17147197 Msa0538040:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG + chr4_1:17147224-17147243 Msa0538040:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG + chr4_1:17147231-17147250 Msa0538040:CDS 45.0%
AACCGTGAAAGCTATGAGTG+AGG + chr4_1:17147291-17147310 Msa0538040:CDS 45.0%
GAAAGCTATGAGTGAGGTAG+AGG + chr4_1:17147297-17147316 Msa0538040:CDS 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG + chr4_1:17147318-17147337 Msa0538040:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG + chr4_1:17147452-17147471 Msa0538040:CDS 45.0%
TTGCAGAATCATCACTTCCC+GGG - chr4_1:17147522-17147541 Msa0538050:three_prime_UTR 45.0%
CTTGCAGAATCATCACTTCC+CGG - chr4_1:17147523-17147542 Msa0538050:three_prime_UTR 45.0%
GGGAAGTGATGATTCTGCAA+GGG + chr4_1:17147522-17147541 Msa0538040:CDS 45.0%
! TTTGACGAACTTTGAGGACC+CGG - chr4_1:17147556-17147575 Msa0538050:three_prime_UTR 45.0%
AGTACCAAGTTCAAGAGAGC+TGG + chr4_1:17147642-17147661 Msa0538040:CDS 45.0%
TTCACCAGCTCTCTTGAACT+TGG - chr4_1:17147649-17147668 Msa0538050:three_prime_UTR 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG - chr4_1:17147675-17147694 Msa0538050:three_prime_UTR 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG - chr4_1:17147676-17147695 Msa0538050:three_prime_UTR 45.0%
! GCACTGAAATCAGGTTCTGA+GGG - chr4_1:17147682-17147701 Msa0538050:three_prime_UTR 45.0%
! AGCACTGAAATCAGGTTCTG+AGG - chr4_1:17147683-17147702 Msa0538050:three_prime_UTR 45.0%
!!! AATTGAATATAAATAAATTT+TGG - chr4_1:17145600-17145619 None:intergenic 5.0%
!! CTTAACTCTCTGGTTCTCGG+GGG + chr4_1:17144118-17144137 Msa0538040:intron 50.0%
!! TTAACTCTCTGGTTCTCGGG+GGG + chr4_1:17144119-17144138 Msa0538040:intron 50.0%
TTCCAGTCAAGATCCGAACC+TGG + chr4_1:17144202-17144221 Msa0538040:intron 50.0%
ATCCAGGTTCGGATCTTGAC+TGG - chr4_1:17144207-17144226 None:intergenic 50.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG - chr4_1:17144548-17144567 None:intergenic 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG - chr4_1:17144549-17144568 None:intergenic 50.0%
GTATCTCAAACCTGTGGCAG+TGG - chr4_1:17145034-17145053 None:intergenic 50.0%
TTATAGCGCAAGACGCATGG+AGG + chr4_1:17145165-17145184 Msa0538040:CDS 50.0%
CTTCTGACTAGTACCTACCC+TGG + chr4_1:17146466-17146485 Msa0538040:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG + chr4_1:17146668-17146687 Msa0538040:CDS 50.0%
CTGACTCTTTCCCTTAGGTC+CGG - chr4_1:17146750-17146769 Msa0538050:three_prime_UTR 50.0%
AGCGTCTGACTCTTTCCCTT+AGG - chr4_1:17146755-17146774 Msa0538050:three_prime_UTR 50.0%
GACGCTACGACCAATACAAG+TGG + chr4_1:17146769-17146788 Msa0538040:CDS 50.0%
TACAAGTGGTTGAACACCCG+AGG + chr4_1:17146783-17146802 Msa0538040:CDS 50.0%
ACAAGTGGTTGAACACCCGA+GGG + chr4_1:17146784-17146803 Msa0538040:CDS 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG - chr4_1:17147259-17147278 Msa0538050:three_prime_UTR 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG + chr4_1:17147458-17147477 Msa0538040:CDS 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG + chr4_1:17147464-17147483 Msa0538040:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG + chr4_1:17147468-17147487 Msa0538040:CDS 50.0%
CGGGAAGTGATGATTCTGCA+AGG + chr4_1:17147521-17147540 Msa0538040:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG + chr4_1:17147528-17147547 Msa0538040:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG + chr4_1:17147607-17147626 Msa0538040:CDS 50.0%
CAATGAAGGCGGTGCGGAAT+TGG - chr4_1:17144904-17144923 None:intergenic 55.0%
AAGGTGCAATGAAGGCGGTG+CGG - chr4_1:17144910-17144929 None:intergenic 55.0%
! CGCAAGACGCATGGAGGTAT+TGG + chr4_1:17145171-17145190 Msa0538040:CDS 55.0%
TGGGCACATGCACCTCTACA+TGG - chr4_1:17146216-17146235 Msa0538050:intron 55.0%
GACTAGTACCTACCCTGGAG+AGG + chr4_1:17146471-17146490 Msa0538040:CDS 55.0%
CGGTTCGTCTGGAGGAAATG+AGG + chr4_1:17146676-17146695 Msa0538040:CDS 55.0%
GCGATGATGCATCCACTGCT+CGG - chr4_1:17146718-17146737 Msa0538050:three_prime_UTR 55.0%
GCATCATCGCTTGCTTCCTC+CGG + chr4_1:17146728-17146747 Msa0538040:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG + chr4_1:17146737-17146756 Msa0538040:CDS 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG - chr4_1:17146747-17146766 Msa0538050:three_prime_UTR 55.0%
CAAGTGGTTGAACACCCGAG+GGG + chr4_1:17146785-17146804 Msa0538040:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG + chr4_1:17147534-17147553 Msa0538040:CDS 55.0%
! CCTTTTCCTATTCCCGGCCG+CGG + chr4_1:17144533-17144552 Msa0538040:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG + chr4_1:17144534-17144553 Msa0538040:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG - chr4_1:17144542-17144561 None:intergenic 60.0%
GTCTGTACTGCCACTGCCAC+AGG + chr4_1:17145021-17145040 Msa0538040:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG + chr4_1:17146703-17146722 Msa0538040:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG + chr4_1:17146736-17146755 Msa0538040:CDS 60.0%
AAGGCGAAGGCGAAGGAAGG+AGG + chr4_1:17147471-17147490 Msa0538040:CDS 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG + chr4_1:17147535-17147554 Msa0538040:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG - chr4_1:17144536-17144555 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 17144001 17147720 17144001 ID=Msa0538040;Name=Msa0538040
chr4_1 mRNA 17144001 17147720 17144001 ID=Msa0538040-mRNA-1;Parent=Msa0538040;Name=Msa0538040-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|0.85|1|1|7|0|744
chr4_1 exon 17144001 17144059 17144001 ID=Msa0538040-mRNA-1:exon:5067;Parent=Msa0538040-mRNA-1
chr4_1 exon 17144451 17145042 17144451 ID=Msa0538040-mRNA-1:exon:5068;Parent=Msa0538040-mRNA-1
chr4_1 exon 17145145 17145357 17145145 ID=Msa0538040-mRNA-1:exon:5069;Parent=Msa0538040-mRNA-1
chr4_1 exon 17145756 17146066 17145756 ID=Msa0538040-mRNA-1:exon:5070;Parent=Msa0538040-mRNA-1
chr4_1 exon 17146450 17146561 17146450 ID=Msa0538040-mRNA-1:exon:5071;Parent=Msa0538040-mRNA-1
chr4_1 exon 17146657 17146893 17146657 ID=Msa0538040-mRNA-1:exon:5072;Parent=Msa0538040-mRNA-1
chr4_1 exon 17147010 17147720 17147010 ID=Msa0538040-mRNA-1:exon:5073;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17144001 17144059 17144001 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17144451 17145042 17144451 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17145145 17145357 17145145 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17145756 17146066 17145756 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17146450 17146561 17146450 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17146657 17146893 17146657 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
chr4_1 CDS 17147010 17147720 17147010 ID=Msa0538040-mRNA-1:cds;Parent=Msa0538040-mRNA-1
Gene Sequence

>Msa0538040

ATGTTTGACTCTGGTTACAAGTCACAGTATATTGAAGGTCAGAAGGAGAAATTTGTGAGGTTGGATGATTTGGATTCCAATTCGTCAATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCTCAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>Msa0538040

MFDSGYKSQYIEGQKEKFVRLDDLDSNSSMPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE*