AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625910 MtrunA17_Chr4g0001481 97.177 744 20 1 1 744 1 743 0.0 1498
Msa0625910 MtrunA17_Chr4g0073941 64.793 747 235 11 1 739 1 727 0.0 951
Msa0625910 MtrunA17_Chr1g0158861 49.732 746 326 12 14 744 5 716 0.0 715
Msa0625910 MtrunA17_Chr3g0126541 50.270 740 321 9 14 739 4 710 0.0 713
Msa0625910 MtrunA17_Chr2g0305771 52.199 682 293 6 82 744 24 691 0.0 708
Msa0625910 MtrunA17_Chr3g0139071 52.246 668 295 7 89 739 61 721 0.0 677
Msa0625910 MtrunA17_Chr2g0326871 49.517 725 327 14 20 738 10 701 0.0 677
Msa0625910 MtrunA17_Chr7g0275581 48.780 738 334 10 17 739 7 715 0.0 666
Msa0625910 MtrunA17_Chr4g0028861 55.150 602 257 7 69 666 90 682 0.0 664
Msa0625910 MtrunA17_Chr1g0181121 51.420 669 293 13 89 742 32 683 0.0 656
Msa0625910 MtrunA17_Chr7g0228181 51.156 649 294 10 100 738 43 678 0.0 655
Msa0625910 MtrunA17_Chr8g0347121 45.407 762 336 11 14 737 4 723 0.0 630
Msa0625910 MtrunA17_Chr3g0125651 47.661 684 317 12 90 743 20 692 0.0 630
Msa0625910 MtrunA17_Chr6g0481021 40.956 586 310 15 101 666 123 692 8.42e-128 396
Msa0625910 MtrunA17_Chr6g0480851 38.889 594 327 14 101 678 184 757 6.77e-121 380
Msa0625910 MtrunA17_Chr6g0480671 39.626 588 324 12 100 672 192 763 8.79e-121 380
Msa0625910 MtrunA17_Chr6g0480781 34.429 700 400 18 16 686 90 759 3.94e-117 370
Msa0625910 MtrunA17_Chr8g0360821 35.777 573 348 10 103 664 64 627 6.65e-110 348
Msa0625910 MtrunA17_Chr7g0218341 36.333 589 354 9 86 666 102 677 4.37e-109 347
Msa0625910 MtrunA17_Chr6g0480741 35.495 586 335 15 100 664 114 677 1.96e-97 316
Msa0625910 MtrunA17_Chr6g0480681 33.750 640 349 19 101 689 115 730 1.89e-91 302
Msa0625910 MtrunA17_Chr2g0318561 36.245 458 273 9 217 664 2 450 5.78e-91 293
Msa0625910 MtrunA17_Chr5g0395421 38.162 359 186 11 299 642 18 355 2.32e-60 208
Msa0625910 MtrunA17_Chr6g0480771 39.113 248 128 6 425 659 12 249 3.62e-42 155
Msa0625910 MtrunA17_Chr2g0326861 40.000 205 114 4 176 378 14 211 6.38e-40 146
Msa0625910 MtrunA17_Chr6g0480841 41.237 194 94 5 479 659 2 188 1.52e-34 131
Msa0625910 MtrunA17_Chr5g0395411 35.821 201 114 4 101 300 172 358 2.03e-32 129
Msa0625910 MtrunA17_Chr2g0326851 43.262 141 62 5 604 738 3 131 2.59e-27 107
Msa0625910 MtrunA17_Chr6g0480751 36.567 134 85 0 100 233 97 230 2.56e-23 99.4
Msa0625910 MtrunA17_Chr6g0480791 41.176 119 69 1 101 218 172 290 7.06e-22 96.7
Msa0625910 MtrunA17_Chr4g0063141 25.366 205 150 2 379 582 239 441 3.81e-17 86.3
Msa0625910 MtrunA17_Chr3g0138671 25.616 203 148 2 380 582 243 442 2.61e-16 83.2
Msa0625910 MtrunA17_Chr2g0277651 24.020 204 152 2 379 582 262 462 5.63e-15 79.0
Msa0625910 MtrunA17_Chr5g0434371 24.194 186 138 2 374 559 277 459 1.12e-12 71.6
Msa0625910 MtrunA17_Chr6g0480761 28.859 149 97 5 243 389 24 165 2.22e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625910 AT1G19780.1 68.519 756 215 10 6 744 2 751 0.0 1024
Msa0625910 AT1G15990.1 68.133 750 209 14 6 739 2 737 0.0 999
Msa0625910 AT2G23980.9 62.914 755 253 10 1 739 1 744 0.0 955
Msa0625910 AT2G23980.10 62.914 755 253 10 1 739 1 744 0.0 955
Msa0625910 AT2G23980.1 62.914 755 253 10 1 739 1 744 0.0 955
Msa0625910 AT2G23980.4 62.914 755 253 10 1 739 1 744 0.0 955
Msa0625910 AT2G23980.5 62.914 755 253 10 1 739 1 744 0.0 955
Msa0625910 AT4G30560.1 63.431 752 239 9 1 739 1 729 0.0 949
Msa0625910 AT4G30560.4 63.431 752 239 9 1 739 1 729 0.0 949
Msa0625910 AT5G57940.1 64.441 734 238 8 11 739 1 716 0.0 943
Msa0625910 AT5G57940.2 64.441 734 238 8 11 739 1 716 0.0 943
Msa0625910 AT2G23980.3 61.054 778 253 11 1 739 1 767 0.0 941
Msa0625910 AT2G23980.2 61.054 778 253 11 1 739 1 767 0.0 941
Msa0625910 AT2G23980.8 61.054 778 253 11 1 739 1 767 0.0 941
Msa0625910 AT2G23980.7 61.054 778 253 11 1 739 1 767 0.0 941
Msa0625910 AT5G57940.3 63.896 734 235 8 11 739 1 709 0.0 930
Msa0625910 AT2G23980.6 63.049 728 246 9 24 739 1 717 0.0 928
Msa0625910 AT4G30560.5 63.636 726 231 8 24 739 1 703 0.0 924
Msa0625910 AT4G30560.2 63.636 726 231 8 24 739 1 703 0.0 924
Msa0625910 AT4G30560.3 63.636 726 231 8 24 739 1 703 0.0 924
Msa0625910 AT1G19780.2 70.425 541 145 7 217 744 2 540 0.0 754
Msa0625910 AT1G01340.2 53.707 661 279 6 92 739 59 705 0.0 718
Msa0625910 AT1G01340.1 53.707 661 279 6 92 739 54 700 0.0 717
Msa0625910 AT4G01010.1 51.489 705 293 11 53 741 24 695 0.0 704
Msa0625910 AT4G01010.2 51.489 705 293 11 53 741 24 695 0.0 704
Msa0625910 AT5G53130.1 49.730 742 326 10 14 739 4 714 0.0 692
Msa0625910 AT2G28260.1 50.946 687 305 8 55 739 21 677 0.0 683
Msa0625910 AT2G24610.1 52.444 675 287 11 89 739 60 724 0.0 682
Msa0625910 AT2G24610.2 52.444 675 287 11 89 739 75 739 0.0 681
Msa0625910 AT2G28260.2 52.024 667 289 7 74 739 1 637 0.0 680
Msa0625910 AT4G30360.1 51.672 658 299 10 99 743 69 720 0.0 655
Msa0625910 AT2G46430.1 47.241 743 327 12 14 737 5 701 0.0 653
Msa0625910 AT2G46430.2 47.241 743 327 12 14 737 5 701 0.0 653
Msa0625910 AT5G14870.1 49.552 670 303 9 98 738 37 700 0.0 647
Msa0625910 AT3G48010.1 47.640 678 303 12 98 737 41 704 0.0 623
Msa0625910 AT2G46440.1 43.339 623 309 13 99 706 28 621 4.09e-162 483
Msa0625910 AT2G46450.4 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0625910 AT2G46450.1 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0625910 AT2G46450.3 37.557 655 325 17 99 735 15 603 1.43e-140 427
Msa0625910 AT2G46450.2 37.557 655 325 17 99 735 28 616 1.93e-140 427
Msa0625910 AT2G46440.2 47.097 465 208 10 256 706 3 443 3.46e-130 394
Msa0625910 AT3G17690.1 37.563 591 338 14 90 667 147 719 4.33e-119 375
Msa0625910 AT3G17700.1 37.544 570 328 10 101 658 192 745 3.87e-115 365
Msa0625910 AT5G15410.1 36.678 578 347 9 94 658 106 677 2.88e-108 346
Msa0625910 AT5G15410.2 36.678 578 347 9 94 658 105 676 3.08e-108 346
Msa0625910 AT5G54250.2 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0625910 AT5G54250.1 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0625910 AT5G54250.4 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0625910 AT3G17690.2 37.897 504 291 10 90 589 147 632 1.03e-105 337
Msa0625910 AT3G17700.2 37.576 495 292 7 101 594 192 670 5.16e-103 331
Msa0625910 AT5G54250.3 33.854 576 360 9 100 664 78 643 4.18e-102 329
Msa0625910 AT3G17690.3 35.433 381 231 8 90 466 147 516 2.86e-66 228
Msa0625910 AT4G22200.2 24.020 204 152 2 379 582 263 463 2.29e-16 83.6
Msa0625910 AT4G22200.1 24.020 204 152 2 379 582 263 463 5.32e-16 82.8
Msa0625910 AT5G37500.1 21.702 470 273 13 99 561 55 436 8.12e-15 79.0
Msa0625910 AT5G37500.2 21.489 470 274 13 99 561 55 436 1.01e-14 79.0
Msa0625910 AT5G37500.4 20.726 468 280 12 99 561 55 436 1.32e-14 78.2
Msa0625910 AT5G37500.3 20.833 408 252 9 155 561 45 382 3.13e-14 77.0
Msa0625910 AT2G26650.1 23.902 205 153 2 379 582 239 441 3.88e-14 77.0
Msa0625910 AT2G26650.2 23.902 205 153 2 379 582 134 336 5.30e-14 76.3
Msa0625910 AT5G46240.1 22.727 198 152 1 379 576 246 442 5.62e-14 76.3
Msa0625910 AT3G02850.2 20.902 488 293 14 83 563 56 457 1.04e-13 75.5
Msa0625910 AT3G02850.1 20.902 488 293 14 83 563 56 457 1.27e-13 75.1
Msa0625910 AT3G02850.3 20.697 488 294 14 83 563 56 457 1.58e-13 74.7
Msa0625910 AT4G32650.2 23.153 203 153 2 380 582 276 475 1.58e-12 71.2
Msa0625910 AT4G32650.3 23.153 203 153 2 380 582 276 475 2.14e-12 71.2
Msa0625910 AT4G32650.1 23.153 203 153 2 380 582 276 475 2.27e-12 70.9

Find 148 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4_3:-5223249 Msa0625910:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4_3:+5222494 Msa0625900:three_prime_UTR
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4_3:-5223705 Msa0625910:intron
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4_3:-5222191 Msa0625910:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4_3:-5224822 Msa0625910:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4_3:+5223726 Msa0625900:three_prime_UTR
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4_3:-5222216 Msa0625910:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4_3:-5222298 Msa0625910:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4_3:+5225083 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4_3:+5222913 Msa0625900:three_prime_UTR
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4_3:-5225168 Msa0625910:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4_3:+5224923 None:intergenic
ATGATGTAGGTTGGATGATT+TGG 0.281948 4_3:-5225303 Msa0625910:intron
CAGAGAAGACTTACATAGTT+AGG 0.283571 4_3:-5222667 Msa0625910:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4_3:-5222947 Msa0625910:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4_3:+5223247 Msa0625900:three_prime_UTR
GATATTTCATTATTGACTTC+TGG 0.297261 4_3:-5224746 Msa0625910:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4_3:-5222215 Msa0625910:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4_3:+5223741 Msa0625900:three_prime_UTR
CATAGACAGTAACAAAATTT+GGG 0.315147 4_3:+5223727 Msa0625900:three_prime_UTR
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4_3:-5223221 Msa0625910:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4_3:+5224844 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4_3:-5223248 Msa0625910:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4_3:+5222078 Msa0625900:three_prime_UTR
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4_3:-5224579 Msa0625910:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4_3:+5222077 Msa0625900:three_prime_UTR
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4_3:+5225263 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4_3:-5225143 Msa0625910:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4_3:-5223085 Msa0625910:CDS
GCTAGATGGCATTGACGAAT+TGG 0.374592 4_3:+5225277 None:intergenic
AATGGTACGGTTCATACCTT+TGG 0.384471 4_3:-5224507 Msa0625910:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4_3:-5222163 Msa0625910:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4_3:+5222062 Msa0625900:three_prime_UTR
ATGAACAACATCTCATCAAC+TGG 0.395916 4_3:+5222635 Msa0625900:three_prime_UTR
AAGATAAGAATCTTCTGTAT+TGG 0.412931 4_3:-5225040 Msa0625910:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4_3:+5222230 Msa0625900:three_prime_UTR
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4_3:-5222143 Msa0625910:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4_3:+5222473 Msa0625900:three_prime_UTR
TACCTCACTCATAGCTTTCA+CGG 0.425446 4_3:+5222457 Msa0625900:three_prime_UTR
TACGATTTGTGGTTGAAATA+CGG 0.428941 4_3:+5224954 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4_3:-5224724 Msa0625910:intron
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4_3:-5225115 Msa0625910:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4_3:+5222348 Msa0625900:three_prime_UTR
GTGCCATGTACTATTTGATC+TGG 0.449942 4_3:-5224433 Msa0625910:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4_3:+5222971 Msa0625900:three_prime_UTR
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4_3:+5223266 Msa0625900:three_prime_UTR
TGGCTAAACAATACGATTTG+TGG 0.457799 4_3:+5224943 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4_3:+5224512 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4_3:-5223022 Msa0625910:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4_3:-5223014 Msa0625910:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4_3:-5224795 Msa0625910:CDS
TACAGAAGATTCTTATCTTG+TGG 0.474072 4_3:+5225044 None:intergenic
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4_3:+5225212 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4_3:+5222104 Msa0625900:three_prime_UTR
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4_3:+5223003 Msa0625900:three_prime_UTR
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4_3:+5222998 Msa0625900:three_prime_UTR
TTCTTCACAATGTTTGACTC+TGG 0.491771 4_3:-5225754 None:intergenic
AGAGAAGACTTACATAGTTA+GGG 0.493349 4_3:-5222666 Msa0625910:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4_3:+5224777 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4_3:-5223963 Msa0625910:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4_3:-5223938 Msa0625910:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4_3:-5222741 Msa0625910:CDS
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4_3:-5222432 Msa0625910:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4_3:-5223910 Msa0625910:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4_3:-5222879 Msa0625910:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4_3:-5225114 Msa0625910:CDS
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4_3:+5224830 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4_3:-5222340 Msa0625910:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4_3:+5223088 Msa0625900:three_prime_UTR
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4_3:+5224714 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4_3:-5223065 Msa0625910:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4_3:-5222349 Msa0625910:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4_3:-5224588 Msa0625910:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4_3:-5222519 Msa0625910:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4_3:-5222222 Msa0625910:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4_3:-5224816 Msa0625910:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4_3:-5223308 Msa0625910:intron
TACAAGTCACAGTATATTGA+AGG 0.537328 4_3:-5225730 Msa0625910:CDS
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4_3:-5222453 Msa0625910:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4_3:-5222626 Msa0625910:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4_3:-5222599 Msa0625910:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4_3:-5223886 Msa0625910:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4_3:+5222680 Msa0625900:three_prime_UTR
TAGACACATACATAGTAGAC+AGG 0.544177 4_3:-5222384 Msa0625910:CDS
ACAGTATATTGAAGGTCAGA+AGG 0.547235 4_3:-5225722 Msa0625910:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4_3:-5223082 Msa0625910:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4_3:-5222878 Msa0625910:intron
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4_3:+5222071 Msa0625900:three_prime_UTR
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4_3:-5222572 Msa0625910:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4_3:-5223013 Msa0625910:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4_3:-5225125 Msa0625910:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4_3:+5222070 Msa0625900:three_prime_UTR
TTTGACGAACTTTGAGGACC+CGG 0.557037 4_3:+5222197 Msa0625900:three_prime_UTR
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4_3:-5223937 Msa0625910:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4_3:+5222231 Msa0625900:three_prime_UTR
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4_3:-5223074 Msa0625910:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4_3:-5222108 Msa0625910:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4_3:+5224819 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4_3:-5223210 Msa0625910:intron
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4_3:-5222587 Msa0625910:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4_3:-5224613 Msa0625910:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4_3:-5223307 Msa0625910:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4_3:-5225269 Msa0625910:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4_3:-5222282 Msa0625910:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4_3:-5223247 Msa0625910:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4_3:-5225113 Msa0625910:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4_3:-5222286 Msa0625910:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4_3:-5222313 Msa0625910:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4_3:-5222292 Msa0625910:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4_3:-5222249 Msa0625910:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4_3:+5223035 Msa0625900:three_prime_UTR
ATATACCAGGGTATGACAGT+TGG 0.606713 4_3:-5223873 Msa0625910:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4_3:-5224616 Msa0625910:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4_3:-5223279 Msa0625910:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4_3:-5222596 Msa0625910:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4_3:-5223094 Msa0625910:CDS
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4_3:-5222279 Msa0625910:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4_3:+5223006 Msa0625900:three_prime_UTR
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4_3:-5223047 Msa0625910:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4_3:+5223271 Msa0625900:three_prime_UTR
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4_3:-5222526 Msa0625910:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4_3:-5222229 Msa0625910:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4_3:-5222662 Msa0625910:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4_3:-5222967 Msa0625910:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4_3:-5222981 Msa0625910:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4_3:+5223267 Msa0625900:three_prime_UTR
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4_3:-5225179 Msa0625910:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4_3:+5222737 Msa0625900:three_prime_UTR
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4_3:-5222228 Msa0625910:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4_3:+5224430 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4_3:+5223868 Msa0625900:three_prime_UTR
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4_3:-5225268 Msa0625910:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4_3:+5223728 Msa0625900:three_prime_UTR
GTCAGAAGGAGAAATTTGTG+AGG 0.668583 4_3:-5225708 Msa0625910:intron
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4_3:-5223999 Msa0625910:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4_3:-5223284 Msa0625910:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4_3:+5224833 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4_3:-5222966 Msa0625910:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4_3:-5222459 Msa0625910:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4_3:-5222339 Msa0625910:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4_3:-5222525 Msa0625910:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4_3:+5224838 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4_3:-5222248 Msa0625910:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4_3:+5223092 Msa0625900:three_prime_UTR
TCTATATTCAAGCTGCATGG+AGG 0.707083 4_3:-5222310 Msa0625910:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4_3:-5224585 Msa0625910:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4_3:-5223885 Msa0625910:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4_3:-5222965 Msa0625910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTAAAATTCTATAAATTA+TGG + chr4_3:5224337-5224356 None:intergenic 10.0%
!!! AACTATTATTTTGTAGATAA+CGG - chr4_3:5223787-5223806 Msa0625910:CDS 15.0%
!! AAATATATATGTATTAGTGT+CGG - chr4_3:5224215-5224234 Msa0625910:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG + chr4_3:5224386-5224405 None:intergenic 15.0%
!!! TTGTGAGGTATTTTTATTTT+TGG - chr4_3:5222104-5222123 Msa0625910:CDS 20.0%
!!! TGTGAGGTATTTTTATTTTT+GGG - chr4_3:5222105-5222124 Msa0625910:CDS 20.0%
!!! TATTGGTTAATTTAAGAACA+TGG - chr4_3:5222358-5222377 Msa0625910:CDS 20.0%
!!! TTATATTCCACTTTAAGTTT+AGG - chr4_3:5222417-5222436 Msa0625910:CDS 20.0%
!!! TATATTCCACTTTAAGTTTA+GGG - chr4_3:5222418-5222437 Msa0625910:CDS 20.0%
!!! ACTATTATTTTGTAGATAAC+GGG - chr4_3:5223788-5223807 Msa0625910:CDS 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG + chr4_3:5224178-5224197 None:intergenic 20.0%
!! GAATCAAAGCATATTTATAA+AGG + chr4_3:5224396-5224415 None:intergenic 20.0%
!! ATGTTTACAGTAAAAACTTT+CGG + chr4_3:5224617-5224636 None:intergenic 20.0%
! GTATAACATATCAATGTGAT+TGG - chr4_3:5222127-5222146 Msa0625910:CDS 25.0%
! CTTCAAAAAAAAAAACCACT+TGG - chr4_3:5222332-5222351 Msa0625910:CDS 25.0%
! AGATGCTTATGTTAACAAAT+TGG + chr4_3:5222454-5222473 Msa0625900:three_prime_UTR 25.0%
! AAGATAAGAATCTTCTGTAT+TGG - chr4_3:5222757-5222776 Msa0625910:intron 25.0%
!!! GATATTTCATTATTGACTTC+TGG - chr4_3:5223051-5223070 Msa0625910:CDS 25.0%
! CAACAAACATTAAGACATAT+TGG + chr4_3:5223169-5223188 Msa0625900:intron 25.0%
! TCTTAAGAAATGAACTTACA+TGG + chr4_3:5223404-5223423 Msa0625900:intron 25.0%
! TATCATTCACATATTCTCTT+TGG - chr4_3:5223519-5223538 Msa0625910:intron 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG + chr4_3:5223563-5223582 Msa0625900:intron 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG - chr4_3:5223563-5223582 Msa0625910:intron 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG + chr4_3:5223595-5223614 Msa0625900:intron 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG + chr4_3:5223596-5223615 Msa0625900:intron 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG + chr4_3:5223636-5223655 Msa0625900:intron 25.0%
! AATTCACATTTCTTGTATTG+TGG - chr4_3:5223887-5223906 Msa0625910:CDS 25.0%
! ATAGACAGTAACAAAATTTG+GGG + chr4_3:5224072-5224091 None:intergenic 25.0%
! CATAGACAGTAACAAAATTT+GGG + chr4_3:5224073-5224092 None:intergenic 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG + chr4_3:5224165-5224184 None:intergenic 25.0%
! TTCCATAGTAATAGCTATTA+TGG - chr4_3:5224548-5224567 Msa0625910:CDS 25.0%
! TCCATAGTAATAGCTATTAT+GGG - chr4_3:5224549-5224568 Msa0625910:CDS 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG + chr4_3:5224887-5224906 None:intergenic 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG - chr4_3:5224918-5224937 Msa0625910:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG - chr4_3:5224919-5224938 Msa0625910:CDS 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG + chr4_3:5225005-5225024 None:intergenic 25.0%
! GTGTATGACATATATTATTC+AGG - chr4_3:5225035-5225054 Msa0625910:CDS 25.0%
TACAAGTCACAGTATATTGA+AGG - chr4_3:5222067-5222086 Msa0625910:CDS 30.0%
! GACTCTATTAATCTGGATTT+TGG - chr4_3:5222199-5222218 Msa0625910:CDS 30.0%
!! TATTAATCTGGATTTTGGTC+AGG - chr4_3:5222204-5222223 Msa0625910:CDS 30.0%
CTCGATAGATTTGTCATTAA+CGG - chr4_3:5222284-5222303 Msa0625910:CDS 30.0%
!! AAAAAACCACTTGGTTCTAT+TGG - chr4_3:5222341-5222360 Msa0625910:CDS 30.0%
AATTAACCAATAGAACCAAG+TGG + chr4_3:5222350-5222369 Msa0625900:three_prime_UTR 30.0%
TCTTTCAGACTAATATACAG+TGG - chr4_3:5222471-5222490 Msa0625910:CDS 30.0%
TAATATACAGTGGATGATGT+AGG - chr4_3:5222481-5222500 Msa0625910:CDS 30.0%
!! TCAGATGGACTAATGAATAT+TGG - chr4_3:5222654-5222673 Msa0625910:CDS 30.0%
TACAGAAGATTCTTATCTTG+TGG + chr4_3:5222756-5222775 Msa0625900:intron 30.0%
TACGGAAGATAGAAAAAGAA+AGG + chr4_3:5222828-5222847 Msa0625900:intron 30.0%
TACGATTTGTGGTTGAAATA+CGG + chr4_3:5222846-5222865 Msa0625900:intron 30.0%
! AATATTTTCCGAGAATGGTA+CGG - chr4_3:5223277-5223296 Msa0625910:CDS 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG + chr4_3:5223309-5223328 Msa0625900:intron 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG - chr4_3:5223342-5223361 Msa0625910:intron 30.0%
ATACCAGATCAAATAGTACA+TGG + chr4_3:5223370-5223389 Msa0625900:intron 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG - chr4_3:5223479-5223498 Msa0625910:intron 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG + chr4_3:5223484-5223503 Msa0625900:intron 30.0%
GGAAGTTTCCATAGAAAAAT+TGG + chr4_3:5223574-5223593 Msa0625900:intron 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG + chr4_3:5223635-5223654 Msa0625900:intron 30.0%
TTGTAGATATTCTGACATAG+AGG - chr4_3:5223691-5223710 Msa0625910:intron 30.0%
GTTAGAATAGATTACGCTAT+AGG - chr4_3:5223713-5223732 Msa0625910:CDS 30.0%
AAAGCATGTAAAGAAGTTGA+GGG - chr4_3:5223860-5223879 Msa0625910:CDS 30.0%
!! GATAACTCAGCTTTTAACTA+TGG - chr4_3:5223995-5224014 Msa0625910:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG + chr4_3:5224059-5224078 None:intergenic 30.0%
CCATAGACAGTAACAAAATT+TGG + chr4_3:5224074-5224093 None:intergenic 30.0%
! CCAAATTTTGTTACTGTCTA+TGG - chr4_3:5224071-5224090 Msa0625910:intron 30.0%
! AATTTTGTTACTGTCTATGG+TGG - chr4_3:5224074-5224093 Msa0625910:intron 30.0%
! ATTTTGTTACTGTCTATGGT+GGG - chr4_3:5224075-5224094 Msa0625910:intron 30.0%
ATGGCTAATGTGAAAAACAT+AGG + chr4_3:5224244-5224263 None:intergenic 30.0%
TCTGATCTTCATATCAAAGT+CGG - chr4_3:5224292-5224311 Msa0625910:intron 30.0%
ATTGTAGATTCACTTGATGT+TGG - chr4_3:5224355-5224374 Msa0625910:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG - chr4_3:5224356-5224375 Msa0625910:intron 30.0%
AAGCAAAACAGAAGGATATT+TGG + chr4_3:5224470-5224489 None:intergenic 30.0%
CCATAGTAATAGCTATTATG+GGG - chr4_3:5224550-5224569 Msa0625910:CDS 30.0%
CCAATTGCATTTATAACACA+AGG + chr4_3:5224680-5224699 None:intergenic 30.0%
CCTTGTGTTATAAATGCAAT+TGG - chr4_3:5224677-5224696 Msa0625910:intron 30.0%
GTCTTCTCTGTATATAAACT+CGG + chr4_3:5225120-5225139 None:intergenic 30.0%
AGAGAAGACTTACATAGTTA+GGG - chr4_3:5225131-5225150 Msa0625910:CDS 30.0%
ATATTACGAATCTGATTACC+CGG - chr4_3:5225548-5225567 Msa0625910:intron 30.0%
ACAGTATATTGAAGGTCAGA+AGG - chr4_3:5222075-5222094 Msa0625910:CDS 35.0%
TTTCCGTTATCTTAACTCTC+TGG - chr4_3:5222160-5222179 Msa0625910:CDS 35.0%
GAACCAGAGAGTTAAGATAA+CGG + chr4_3:5222166-5222185 Msa0625900:three_prime_UTR 35.0%
!! TATCTTAACTCTCTGGTTCT+CGG - chr4_3:5222167-5222186 Msa0625910:CDS 35.0%
GGAAAGAGACTCTATTAATC+TGG - chr4_3:5222192-5222211 Msa0625910:CDS 35.0%
! AATCTATCGAGAGTATATCC+AGG + chr4_3:5222275-5222294 Msa0625900:three_prime_UTR 35.0%
!! TTTTTGAAGAGAGAACCAAG+TGG + chr4_3:5222321-5222340 Msa0625900:three_prime_UTR 35.0%
TGCATACCCTAAACTTAAAG+TGG + chr4_3:5222427-5222446 Msa0625900:three_prime_UTR 35.0%
! ATGATGTAGGTTGGATGATT+TGG - chr4_3:5222494-5222513 Msa0625910:CDS 35.0%
ATTGGAAGATCACTCAAAAG+TGG - chr4_3:5222672-5222691 Msa0625910:CDS 35.0%
!! TGTGACACTTTAAGATCTTC+TGG + chr4_3:5222717-5222736 Msa0625900:three_prime_UTR 35.0%
TGGCTAAACAATACGATTTG+TGG + chr4_3:5222857-5222876 Msa0625900:intron 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG + chr4_3:5222877-5222896 Msa0625900:intron 35.0%
CAAAACATATTGAGCATGGA+TGG + chr4_3:5223108-5223127 Msa0625900:intron 35.0%
TACGCAAAACATATTGAGCA+TGG + chr4_3:5223112-5223131 Msa0625900:intron 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG - chr4_3:5223181-5223200 Msa0625910:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG - chr4_3:5223272-5223291 Msa0625910:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG - chr4_3:5223315-5223334 Msa0625910:intron 35.0%
CATAAGTGATACAGAAGCAA+TGG + chr4_3:5223448-5223467 Msa0625900:intron 35.0%
TCTGTATCACTTATGAAGCT+CGG - chr4_3:5223453-5223472 Msa0625910:intron 35.0%
! GAAGTATCAAGAAGTGTATG+AGG + chr4_3:5223613-5223632 Msa0625900:intron 35.0%
AGAATAGATTACGCTATAGG+CGG - chr4_3:5223716-5223735 Msa0625910:CDS 35.0%
GAAACATTGATCATTCGTGT+CGG + chr4_3:5223762-5223781 Msa0625900:three_prime_UTR 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG - chr4_3:5223859-5223878 Msa0625910:CDS 35.0%
AGTTCAAGTAAGCATATACC+AGG - chr4_3:5223911-5223930 Msa0625910:CDS 35.0%
GTTCAAGTAAGCATATACCA+GGG - chr4_3:5223912-5223931 Msa0625910:CDS 35.0%
! TTTTGTTACTGTCTATGGTG+GGG - chr4_3:5224076-5224095 Msa0625910:intron 35.0%
TGTTATAAAGAGGACTCATG+TGG + chr4_3:5224155-5224174 None:intergenic 35.0%
! TTTTCACATTAGCCATGTAG+AGG - chr4_3:5224248-5224267 Msa0625910:intron 35.0%
ACTGTGAAAAGCAAAACAGA+AGG + chr4_3:5224478-5224497 None:intergenic 35.0%
!! TTTGCTTTTCACAGTACACT+TGG - chr4_3:5224483-5224502 Msa0625910:CDS 35.0%
CCCCATAATAGCTATTACTA+TGG + chr4_3:5224553-5224572 None:intergenic 35.0%
TGCAATGAGAAATCTCAGAA+AGG + chr4_3:5224969-5224988 None:intergenic 35.0%
CAGAGAAGACTTACATAGTT+AGG - chr4_3:5225130-5225149 Msa0625910:CDS 35.0%
AAGACTTACATAGTTAGGGA+AGG - chr4_3:5225135-5225154 Msa0625910:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG + chr4_3:5225165-5225184 None:intergenic 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG - chr4_3:5225252-5225271 Msa0625910:CDS 35.0%
TAGACACATACATAGTAGAC+AGG - chr4_3:5225413-5225432 Msa0625910:intron 35.0%
TCCATTGTTGCGAATAGAAA+CGG + chr4_3:5225452-5225471 None:intergenic 35.0%
CAATCTATATTCAAGCTGCA+TGG - chr4_3:5225484-5225503 Msa0625910:intron 35.0%
TATTACGAATCTGATTACCC+GGG - chr4_3:5225549-5225568 Msa0625910:intron 35.0%
!! CCAAATTTTGACGAACTTTG+AGG + chr4_3:5225609-5225628 None:intergenic 35.0%
CCTCAAAGTTCGTCAAAATT+TGG - chr4_3:5225606-5225625 Msa0625910:intron 35.0%
ATACAACAGTCTATGCTTCT+CGG - chr4_3:5225634-5225653 Msa0625910:intron 35.0%
GTCAGAAGGAGAAATTTGTG+AGG - chr4_3:5222089-5222108 Msa0625910:CDS 40.0%
!! ATCTTAACTCTCTGGTTCTC+GGG - chr4_3:5222168-5222187 Msa0625910:CDS 40.0%
! ATCGAGAGTATATCCAGGTT+CGG + chr4_3:5222270-5222289 Msa0625900:three_prime_UTR 40.0%
ATACAGTGGATGATGTAGGT+TGG - chr4_3:5222485-5222504 Msa0625910:CDS 40.0%
! TGTTTGCCTTTTCCTATTCC+CGG - chr4_3:5222579-5222598 Msa0625910:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG + chr4_3:5222605-5222624 Msa0625900:three_prime_UTR 40.0%
! TCGTCGAAATCATTTCGAGT+CGG - chr4_3:5222618-5222637 Msa0625910:CDS 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG - chr4_3:5222629-5222648 Msa0625910:CDS 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG - chr4_3:5222630-5222649 Msa0625910:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG - chr4_3:5222683-5222702 Msa0625910:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG + chr4_3:5222981-5223000 Msa0625900:three_prime_UTR 40.0%
TCATCTCGAGTATTTGGAAG+AGG - chr4_3:5222981-5223000 Msa0625910:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG - chr4_3:5223184-5223203 Msa0625910:intron 40.0%
AATGGTACGGTTCATACCTT+TGG - chr4_3:5223290-5223309 Msa0625910:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG - chr4_3:5223364-5223383 Msa0625910:intron 40.0%
ACTTCTTGATACTTCCCGAT+TGG - chr4_3:5223618-5223637 Msa0625910:intron 40.0%
ATATACCAGGGTATGACAGT+TGG - chr4_3:5223924-5223943 Msa0625910:CDS 40.0%
GAAGATCAGACACACGATAT+GGG + chr4_3:5224282-5224301 None:intergenic 40.0%
TGAAGATCAGACACACGATA+TGG + chr4_3:5224283-5224302 None:intergenic 40.0%
! TTTTCACAGTACACTTGGTC+AGG - chr4_3:5224488-5224507 Msa0625910:CDS 40.0%
TTTCACAGTACACTTGGTCA+GGG - chr4_3:5224489-5224508 Msa0625910:CDS 40.0%
TGGAAAACAAAACCTCTCCA+GGG + chr4_3:5224533-5224552 None:intergenic 40.0%
ATGGAAAACAAAACCTCTCC+AGG + chr4_3:5224534-5224553 None:intergenic 40.0%
TCTTCTGATTGGAAACATGC+AGG - chr4_3:5224587-5224606 Msa0625910:CDS 40.0%
GACCTACCTTCAATCAATGT+CGG - chr4_3:5224703-5224722 Msa0625910:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG + chr4_3:5224708-5224727 None:intergenic 40.0%
TGGAGGAAATGAGGATCAAA+AGG - chr4_3:5224732-5224751 Msa0625910:CDS 40.0%
! AATTTTCTTCATCGACCCCT+CGG + chr4_3:5224850-5224869 None:intergenic 40.0%
AGGGGTCGATGAAGAAAATT+TGG - chr4_3:5224850-5224869 Msa0625910:CDS 40.0%
GAGATGTTGTTCATCATTCG+TGG - chr4_3:5225171-5225190 Msa0625910:CDS 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG - chr4_3:5225198-5225217 Msa0625910:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG - chr4_3:5225236-5225255 Msa0625910:CDS 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG - chr4_3:5225240-5225259 Msa0625910:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG - chr4_3:5225272-5225291 Msa0625910:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG + chr4_3:5225327-5225346 None:intergenic 40.0%
TACCTCACTCATAGCTTTCA+CGG + chr4_3:5225343-5225362 None:intergenic 40.0%
TCCGTTTCTATTCGCAACAA+TGG - chr4_3:5225448-5225467 Msa0625910:intron 40.0%
ATTCGCAACAATGGAGAACA+TGG - chr4_3:5225457-5225476 Msa0625910:intron 40.0%
TTCGCAACAATGGAGAACAT+GGG - chr4_3:5225458-5225477 Msa0625910:intron 40.0%
TCTATATTCAAGCTGCATGG+AGG - chr4_3:5225487-5225506 Msa0625910:intron 40.0%
!! TCATTGAGAGCACTGAAATC+AGG + chr4_3:5225738-5225757 None:intergenic 40.0%
!! TCTTAACTCTCTGGTTCTCG+GGG - chr4_3:5222169-5222188 Msa0625910:CDS 45.0%
ACGGAAGTTCATCAACCACT+TGG - chr4_3:5222303-5222322 Msa0625910:CDS 45.0%
!! GCTAGATGGCATTGACGAAT+TGG + chr4_3:5222523-5222542 Msa0625900:three_prime_UTR 45.0%
! TCAATGCCATCTAGCTCAAG+TGG - chr4_3:5222528-5222547 Msa0625910:CDS 45.0%
! CAATGCCATCTAGCTCAAGT+GGG - chr4_3:5222529-5222548 Msa0625910:CDS 45.0%
TTCATCCCACTTGAGCTAGA+TGG + chr4_3:5222537-5222556 Msa0625900:three_prime_UTR 45.0%
GGAGTGAAAAAGGGATCAGA+TGG - chr4_3:5222639-5222658 Msa0625910:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG - chr4_3:5222682-5222701 Msa0625910:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG - chr4_3:5222684-5222703 Msa0625910:CDS 45.0%
! AGATGAAGGTGCAATGAAGG+CGG + chr4_3:5222967-5222986 Msa0625900:three_prime_UTR 45.0%
! TCGAGATGAAGGTGCAATGA+AGG + chr4_3:5222970-5222989 Msa0625900:three_prime_UTR 45.0%
GCACCTTCATCTCGAGTATT+TGG - chr4_3:5222975-5222994 Msa0625910:CDS 45.0%
GGTGAACTTGTCATAGATCC+TGG - chr4_3:5223002-5223021 Msa0625910:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG + chr4_3:5223023-5223042 Msa0625900:three_prime_UTR 45.0%
TGGATGGTATCTCAAACCTG+TGG + chr4_3:5223092-5223111 Msa0625900:three_prime_UTR 45.0%
TCTTTATAGCGCAAGACGCA+TGG - chr4_3:5223209-5223228 Msa0625910:intron 45.0%
GGTATGAACCGTACCATTCT+CGG + chr4_3:5223288-5223307 Msa0625900:three_prime_UTR 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG - chr4_3:5223798-5223817 Msa0625910:CDS 45.0%
TAGAACGTAACGACACATGC+TGG - chr4_3:5223834-5223853 Msa0625910:CDS 45.0%
TTTCTCCAACTGTCATACCC+TGG + chr4_3:5223932-5223951 Msa0625900:three_prime_UTR 45.0%
GGTGGGGACTTCAGAATTTA+AGG - chr4_3:5224092-5224111 Msa0625910:intron 45.0%
TTCACAGTACACTTGGTCAG+GGG - chr4_3:5224490-5224509 Msa0625910:CDS 45.0%
AAACAAAACCTCTCCAGGGT+AGG + chr4_3:5224529-5224548 None:intergenic 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG - chr4_3:5224576-5224595 Msa0625910:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG + chr4_3:5224712-5224731 None:intergenic 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG - chr4_3:5224712-5224731 Msa0625910:intron 45.0%
GGGTGTTCAACCACTTGTAT+TGG + chr4_3:5224829-5224848 None:intergenic 45.0%
! ATTTTCTTCATCGACCCCTC+GGG + chr4_3:5224849-5224868 None:intergenic 45.0%
GGTTCCTCTCTTTGCTAACA+TGG - chr4_3:5225056-5225075 Msa0625910:CDS 45.0%
TCGTCCATGTTAGCAAAGAG+AGG + chr4_3:5225063-5225082 None:intergenic 45.0%
!! GAGAGTGTTACTACAGATGG+TGG - chr4_3:5225201-5225220 Msa0625910:CDS 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG - chr4_3:5225210-5225229 Msa0625910:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG - chr4_3:5225225-5225244 Msa0625910:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG - chr4_3:5225271-5225290 Msa0625910:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG - chr4_3:5225278-5225297 Msa0625910:CDS 45.0%
AACCGTGAAAGCTATGAGTG+AGG - chr4_3:5225338-5225357 Msa0625910:intron 45.0%
GAAAGCTATGAGTGAGGTAG+AGG - chr4_3:5225344-5225363 Msa0625910:intron 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG - chr4_3:5225365-5225384 Msa0625910:intron 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG - chr4_3:5225499-5225518 Msa0625910:intron 45.0%
TTGCAGAATCATCACTTCCC+GGG + chr4_3:5225569-5225588 None:intergenic 45.0%
CTTGCAGAATCATCACTTCC+CGG + chr4_3:5225570-5225589 None:intergenic 45.0%
GGGAAGTGATGATTCTGCAA+GGG - chr4_3:5225569-5225588 Msa0625910:intron 45.0%
! TTTGACGAACTTTGAGGACC+CGG + chr4_3:5225603-5225622 None:intergenic 45.0%
AGTACCAAGTTCAAGAGAGC+TGG - chr4_3:5225689-5225708 Msa0625910:intron 45.0%
TTCACCAGCTCTCTTGAACT+TGG + chr4_3:5225696-5225715 None:intergenic 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG + chr4_3:5225722-5225741 None:intergenic 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG + chr4_3:5225723-5225742 None:intergenic 45.0%
! GCACTGAAATCAGGTTCTGA+GGG + chr4_3:5225729-5225748 None:intergenic 45.0%
! AGCACTGAAATCAGGTTCTG+AGG + chr4_3:5225730-5225749 None:intergenic 45.0%
!!! AATTGAATATAAATAAATTT+TGG + chr4_3:5223647-5223666 Msa0625900:intron 5.0%
!! CTTAACTCTCTGGTTCTCGG+GGG - chr4_3:5222170-5222189 Msa0625910:CDS 50.0%
!! TTAACTCTCTGGTTCTCGGG+GGG - chr4_3:5222171-5222190 Msa0625910:CDS 50.0%
TTCCAGTCAAGATCCGAACC+TGG - chr4_3:5222254-5222273 Msa0625910:CDS 50.0%
ATCCAGGTTCGGATCTTGAC+TGG + chr4_3:5222259-5222278 Msa0625900:three_prime_UTR 50.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG + chr4_3:5222600-5222619 Msa0625900:three_prime_UTR 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG + chr4_3:5222601-5222620 Msa0625900:three_prime_UTR 50.0%
GTATCTCAAACCTGTGGCAG+TGG + chr4_3:5223086-5223105 Msa0625900:three_prime_UTR 50.0%
TTATAGCGCAAGACGCATGG+AGG - chr4_3:5223212-5223231 Msa0625910:CDS 50.0%
CTTCTGACTAGTACCTACCC+TGG - chr4_3:5224513-5224532 Msa0625910:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG - chr4_3:5224715-5224734 Msa0625910:intron 50.0%
CTGACTCTTTCCCTTAGGTC+CGG + chr4_3:5224797-5224816 None:intergenic 50.0%
AGCGTCTGACTCTTTCCCTT+AGG + chr4_3:5224802-5224821 None:intergenic 50.0%
GACGCTACGACCAATACAAG+TGG - chr4_3:5224816-5224835 Msa0625910:CDS 50.0%
TACAAGTGGTTGAACACCCG+AGG - chr4_3:5224830-5224849 Msa0625910:CDS 50.0%
ACAAGTGGTTGAACACCCGA+GGG - chr4_3:5224831-5224850 Msa0625910:CDS 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG + chr4_3:5225306-5225325 None:intergenic 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG - chr4_3:5225505-5225524 Msa0625910:intron 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG - chr4_3:5225511-5225530 Msa0625910:intron 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG - chr4_3:5225515-5225534 Msa0625910:intron 50.0%
CGGGAAGTGATGATTCTGCA+AGG - chr4_3:5225568-5225587 Msa0625910:intron 50.0%
! TGATGATTCTGCAAGGGCGT+TGG - chr4_3:5225575-5225594 Msa0625910:intron 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG - chr4_3:5225654-5225673 Msa0625910:intron 50.0%
CAATGAAGGCGGTGCGGAAT+TGG + chr4_3:5222956-5222975 Msa0625900:three_prime_UTR 55.0%
AAGGTGCAATGAAGGCGGTG+CGG + chr4_3:5222962-5222981 Msa0625900:three_prime_UTR 55.0%
! CGCAAGACGCATGGAGGTAT+TGG - chr4_3:5223218-5223237 Msa0625910:CDS 55.0%
TGGGCACATGCACCTCTACA+TGG + chr4_3:5224263-5224282 None:intergenic 55.0%
GACTAGTACCTACCCTGGAG+AGG - chr4_3:5224518-5224537 Msa0625910:CDS 55.0%
CGGTTCGTCTGGAGGAAATG+AGG - chr4_3:5224723-5224742 Msa0625910:intron 55.0%
GCGATGATGCATCCACTGCT+CGG + chr4_3:5224765-5224784 None:intergenic 55.0%
GCATCATCGCTTGCTTCCTC+CGG - chr4_3:5224775-5224794 Msa0625910:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG - chr4_3:5224784-5224803 Msa0625910:CDS 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG + chr4_3:5224794-5224813 None:intergenic 55.0%
CAAGTGGTTGAACACCCGAG+GGG - chr4_3:5224832-5224851 Msa0625910:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG - chr4_3:5225581-5225600 Msa0625910:intron 55.0%
! CCTTTTCCTATTCCCGGCCG+CGG - chr4_3:5222585-5222604 Msa0625910:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG - chr4_3:5222586-5222605 Msa0625910:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG + chr4_3:5222594-5222613 Msa0625900:three_prime_UTR 60.0%
GTCTGTACTGCCACTGCCAC+AGG - chr4_3:5223073-5223092 Msa0625910:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG - chr4_3:5224750-5224769 Msa0625910:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG - chr4_3:5224783-5224802 Msa0625910:CDS 60.0%
AAGGCGAAGGCGAAGGAAGG+AGG - chr4_3:5225518-5225537 Msa0625910:intron 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG - chr4_3:5225582-5225601 Msa0625910:intron 60.0%
CCGCGGCCGGGAATAGGAAA+AGG + chr4_3:5222588-5222607 Msa0625900:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 5222052 5225767 5222052 ID=Msa0625910;Name=Msa0625910
chr4_3 mRNA 5222052 5225767 5222052 ID=Msa0625910-mRNA-1;Parent=Msa0625910;Name=Msa0625910-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|0.85|1|1|7|0|744
chr4_3 exon 5225709 5225767 5225709 ID=Msa0625910-mRNA-1:exon:1465;Parent=Msa0625910-mRNA-1
chr4_3 exon 5224725 5225316 5224725 ID=Msa0625910-mRNA-1:exon:1464;Parent=Msa0625910-mRNA-1
chr4_3 exon 5224415 5224627 5224415 ID=Msa0625910-mRNA-1:exon:1463;Parent=Msa0625910-mRNA-1
chr4_3 exon 5223706 5224016 5223706 ID=Msa0625910-mRNA-1:exon:1462;Parent=Msa0625910-mRNA-1
chr4_3 exon 5223211 5223322 5223211 ID=Msa0625910-mRNA-1:exon:1461;Parent=Msa0625910-mRNA-1
chr4_3 exon 5222879 5223115 5222879 ID=Msa0625910-mRNA-1:exon:1460;Parent=Msa0625910-mRNA-1
chr4_3 exon 5222052 5222762 5222052 ID=Msa0625910-mRNA-1:exon:1459;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5225709 5225767 5225709 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5224725 5225316 5224725 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5224415 5224627 5224415 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5223706 5224016 5223706 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5223211 5223322 5223211 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5222879 5223115 5222879 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
chr4_3 CDS 5222052 5222762 5222052 ID=Msa0625910-mRNA-1:cds;Parent=Msa0625910-mRNA-1
Gene Sequence

>Msa0625910

ATGTTTGACTCTGGTTACAAGTCACAGTATATTGAAGGTCAGAAGGAGAAATTTGTGAGGTTGGATGATTTGGATTCCAATTCGTCAATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCACAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>Msa0625910

MFDSGYKSQYIEGQKEKFVRLDDLDSNSSMPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE*