AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0663570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663570 MtrunA17_Chr4g0001481 97.063 715 20 1 1 715 30 743 0.0 1438
Msa0663570 MtrunA17_Chr4g0073941 67.052 692 204 8 26 710 53 727 0.0 937
Msa0663570 MtrunA17_Chr1g0158861 53.729 657 276 8 68 715 79 716 0.0 713
Msa0663570 MtrunA17_Chr3g0126541 54.671 653 267 7 68 710 77 710 0.0 711
Msa0663570 MtrunA17_Chr2g0305771 52.199 682 293 6 53 715 24 691 0.0 708
Msa0663570 MtrunA17_Chr3g0139071 52.246 668 295 7 60 710 61 721 0.0 676
Msa0663570 MtrunA17_Chr2g0326871 50.000 712 318 14 4 709 22 701 0.0 672
Msa0663570 MtrunA17_Chr7g0275581 51.740 661 287 7 63 710 74 715 0.0 664
Msa0663570 MtrunA17_Chr4g0028861 55.150 602 257 7 40 637 90 682 0.0 663
Msa0663570 MtrunA17_Chr1g0181121 51.420 669 293 13 60 713 32 683 0.0 655
Msa0663570 MtrunA17_Chr7g0228181 51.156 649 294 10 71 709 43 678 0.0 655
Msa0663570 MtrunA17_Chr3g0125651 47.661 684 317 12 61 714 20 692 0.0 629
Msa0663570 MtrunA17_Chr8g0347121 47.230 722 327 11 28 708 15 723 0.0 628
Msa0663570 MtrunA17_Chr6g0481021 40.956 586 310 15 72 637 123 692 4.46e-128 396
Msa0663570 MtrunA17_Chr6g0480851 38.889 594 327 14 72 649 184 757 4.82e-121 379
Msa0663570 MtrunA17_Chr6g0480671 39.626 588 324 12 71 643 192 763 6.80e-121 379
Msa0663570 MtrunA17_Chr6g0480781 36.275 612 343 14 72 657 169 759 1.65e-117 370
Msa0663570 MtrunA17_Chr8g0360821 35.777 573 348 10 74 635 64 627 2.91e-110 348
Msa0663570 MtrunA17_Chr7g0218341 36.333 589 354 9 57 637 102 677 3.80e-109 347
Msa0663570 MtrunA17_Chr6g0480741 35.495 586 335 15 71 635 114 677 9.94e-98 316
Msa0663570 MtrunA17_Chr6g0480681 33.594 640 350 18 72 660 115 730 1.19e-91 301
Msa0663570 MtrunA17_Chr2g0318561 36.245 458 273 9 188 635 2 450 3.31e-91 293
Msa0663570 MtrunA17_Chr5g0395421 38.162 359 186 11 270 613 18 355 1.71e-60 208
Msa0663570 MtrunA17_Chr6g0480771 39.113 248 128 6 396 630 12 249 2.91e-42 155
Msa0663570 MtrunA17_Chr2g0326861 40.000 205 114 4 147 349 14 211 6.64e-40 146
Msa0663570 MtrunA17_Chr6g0480841 41.237 194 94 5 450 630 2 188 1.72e-34 130
Msa0663570 MtrunA17_Chr5g0395411 35.821 201 114 4 72 271 172 358 1.94e-32 129
Msa0663570 MtrunA17_Chr2g0326851 43.262 141 62 5 575 709 3 131 2.52e-27 107
Msa0663570 MtrunA17_Chr6g0480751 36.567 134 85 0 71 204 97 230 2.58e-23 99.4
Msa0663570 MtrunA17_Chr6g0480791 40.164 122 72 1 72 192 172 293 7.06e-22 96.7
Msa0663570 MtrunA17_Chr4g0063141 25.366 205 150 2 350 553 239 441 3.49e-17 86.3
Msa0663570 MtrunA17_Chr3g0138671 25.616 203 148 2 351 553 243 442 2.51e-16 83.2
Msa0663570 MtrunA17_Chr2g0277651 24.762 210 153 3 346 553 256 462 5.56e-15 79.0
Msa0663570 MtrunA17_Chr5g0434371 24.194 186 138 2 345 530 277 459 1.02e-12 71.6
Msa0663570 MtrunA17_Chr6g0480761 28.859 149 97 5 214 360 24 165 2.18e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663570 AT1G19780.1 68.306 732 209 10 1 715 26 751 0.0 987
Msa0663570 AT1G15990.1 67.906 726 209 12 1 710 20 737 0.0 966
Msa0663570 AT2G23980.6 63.333 720 242 8 3 710 8 717 0.0 927
Msa0663570 AT5G57940.3 66.040 692 217 5 23 710 32 709 0.0 927
Msa0663570 AT2G23980.9 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663570 AT2G23980.10 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663570 AT2G23980.1 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663570 AT2G23980.4 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663570 AT2G23980.5 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663570 AT5G57940.1 66.040 692 217 5 23 710 39 716 0.0 926
Msa0663570 AT5G57940.2 66.040 692 217 5 23 710 39 716 0.0 926
Msa0663570 AT4G30560.1 63.928 718 227 7 3 710 34 729 0.0 925
Msa0663570 AT4G30560.4 63.928 718 227 7 3 710 34 729 0.0 925
Msa0663570 AT4G30560.5 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663570 AT4G30560.2 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663570 AT4G30560.3 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663570 AT2G23980.3 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663570 AT2G23980.2 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663570 AT2G23980.8 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663570 AT2G23980.7 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663570 AT1G19780.2 70.425 541 145 7 188 715 2 540 0.0 753
Msa0663570 AT1G01340.2 53.707 661 279 6 63 710 59 705 0.0 717
Msa0663570 AT1G01340.1 53.707 661 279 6 63 710 54 700 0.0 717
Msa0663570 AT4G01010.1 51.489 705 293 11 24 712 24 695 0.0 704
Msa0663570 AT4G01010.2 51.489 705 293 11 24 712 24 695 0.0 704
Msa0663570 AT5G53130.1 52.388 670 285 7 51 710 69 714 0.0 690
Msa0663570 AT2G24610.1 52.444 675 287 11 60 710 60 724 0.0 681
Msa0663570 AT2G28260.1 50.946 687 305 8 26 710 21 677 0.0 681
Msa0663570 AT2G28260.2 52.024 667 289 7 45 710 1 637 0.0 679
Msa0663570 AT2G24610.2 52.444 675 287 11 60 710 75 739 0.0 679
Msa0663570 AT4G30360.1 51.672 658 299 10 70 714 69 720 0.0 655
Msa0663570 AT2G46430.1 50.383 653 287 8 71 708 71 701 0.0 649
Msa0663570 AT2G46430.2 50.383 653 287 8 71 708 71 701 0.0 649
Msa0663570 AT5G14870.1 49.552 670 303 9 69 709 37 700 0.0 647
Msa0663570 AT3G48010.1 47.640 678 303 12 69 708 41 704 0.0 622
Msa0663570 AT2G46440.1 43.339 623 309 13 70 677 28 621 8.88e-163 483
Msa0663570 AT2G46450.4 38.473 655 342 16 70 706 28 639 5.75e-149 449
Msa0663570 AT2G46450.1 38.473 655 342 16 70 706 28 639 5.75e-149 449
Msa0663570 AT2G46450.2 37.557 655 325 17 70 706 28 616 9.70e-141 427
Msa0663570 AT2G46450.3 37.557 655 325 17 70 706 15 603 1.17e-140 426
Msa0663570 AT2G46440.2 47.097 465 208 10 227 677 3 443 1.49e-130 394
Msa0663570 AT3G17690.1 37.563 591 338 14 61 638 147 719 1.77e-119 375
Msa0663570 AT3G17700.1 37.544 570 328 10 72 629 192 745 1.76e-115 365
Msa0663570 AT5G15410.2 36.678 578 347 9 65 629 105 676 1.67e-108 346
Msa0663570 AT5G15410.1 36.678 578 347 9 65 629 106 677 2.23e-108 345
Msa0663570 AT5G54250.2 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663570 AT5G54250.1 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663570 AT5G54250.4 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663570 AT3G17690.2 37.897 504 291 10 61 560 147 632 6.54e-106 337
Msa0663570 AT3G17700.2 37.652 494 291 7 72 564 192 669 2.66e-103 331
Msa0663570 AT5G54250.3 33.854 576 360 9 71 635 78 643 3.90e-102 328
Msa0663570 AT3G17690.3 35.433 381 231 8 61 437 147 516 1.30e-66 229
Msa0663570 AT4G22200.2 24.020 204 152 2 350 553 263 463 1.97e-16 83.6
Msa0663570 AT4G22200.1 24.020 204 152 2 350 553 263 463 5.67e-16 82.8
Msa0663570 AT5G37500.1 21.489 470 274 13 70 532 55 436 1.11e-14 78.6
Msa0663570 AT5G37500.4 20.726 468 280 12 70 532 55 436 1.47e-14 78.2
Msa0663570 AT5G37500.2 21.702 470 273 13 70 532 55 436 1.57e-14 78.2
Msa0663570 AT2G26650.1 23.902 205 153 2 350 553 239 441 2.89e-14 77.4
Msa0663570 AT5G37500.3 20.833 408 252 9 126 532 45 382 3.13e-14 77.0
Msa0663570 AT2G26650.2 23.902 205 153 2 350 553 134 336 4.39e-14 76.6
Msa0663570 AT5G46240.1 22.727 198 152 1 350 547 246 442 6.08e-14 75.9
Msa0663570 AT3G02850.2 20.902 488 293 14 54 534 56 457 1.11e-13 75.1
Msa0663570 AT3G02850.1 20.902 488 293 14 54 534 56 457 1.18e-13 75.1
Msa0663570 AT3G02850.3 24.479 192 140 3 345 534 269 457 2.04e-13 73.9
Msa0663570 AT4G32650.2 23.153 203 153 2 351 553 276 475 1.53e-12 71.2
Msa0663570 AT4G32650.3 23.153 203 153 2 351 553 276 475 1.99e-12 71.2
Msa0663570 AT4G32650.1 23.153 203 153 2 351 553 276 475 2.08e-12 70.9

Find 142 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4_4:+2477350 Msa0663570:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4_4:-2478105 Msa0663580:three_prime_UTR
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4_4:+2476894 Msa0663570:CDS
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4_4:+2478408 Msa0663570:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4_4:+2475771 Msa0663570:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4_4:-2476873 Msa0663580:three_prime_UTR
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4_4:+2478383 Msa0663570:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4_4:+2478301 Msa0663570:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4_4:-2475510 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4_4:-2477686 Msa0663580:three_prime_UTR
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4_4:+2475425 Msa0663570:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4_4:-2475670 None:intergenic
CAGAGAAGACTTACATAGTT+AGG 0.283571 4_4:+2477932 Msa0663570:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4_4:+2477652 Msa0663570:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4_4:-2477352 Msa0663580:three_prime_UTR
GATATTTCATTATTGACTTC+TGG 0.297261 4_4:+2475847 Msa0663570:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4_4:+2478384 Msa0663570:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4_4:-2476858 Msa0663580:three_prime_UTR
CATAGACAGTAACAAAATTT+GGG 0.315147 4_4:-2476872 Msa0663580:three_prime_UTR
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4_4:+2477378 Msa0663570:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4_4:-2475749 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4_4:+2477351 Msa0663570:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4_4:-2478521 Msa0663580:three_prime_UTR
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4_4:+2476021 Msa0663570:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4_4:-2478522 Msa0663580:three_prime_UTR
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4_4:-2475330 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4_4:+2475450 Msa0663570:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4_4:+2477514 Msa0663570:CDS
AATGGTACGGTTCATACCTT+TGG 0.384471 4_4:+2476093 Msa0663570:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4_4:+2478436 Msa0663570:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4_4:-2478537 Msa0663580:three_prime_UTR
ATGAACAACATCTCATCAAC+TGG 0.395916 4_4:-2477964 Msa0663580:three_prime_UTR
AAGATAAGAATCTTCTGTAT+TGG 0.412931 4_4:+2475553 Msa0663570:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4_4:-2478369 Msa0663580:three_prime_UTR
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4_4:+2478456 Msa0663570:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4_4:-2478126 Msa0663580:three_prime_UTR
TACCTCACTCATAGCTTTCA+CGG 0.425446 4_4:-2478142 Msa0663580:three_prime_UTR
TACGATTTGTGGTTGAAATA+CGG 0.428941 4_4:-2475639 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4_4:+2475869 Msa0663570:CDS
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4_4:+2475478 Msa0663570:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4_4:-2478251 Msa0663580:three_prime_UTR
GTGCCATGTACTATTTGATC+TGG 0.449942 4_4:+2476167 Msa0663570:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4_4:-2477628 Msa0663580:three_prime_UTR
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4_4:-2477333 Msa0663580:three_prime_UTR
TGGCTAAACAATACGATTTG+TGG 0.457799 4_4:-2475650 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4_4:-2476088 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4_4:+2477577 Msa0663570:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4_4:+2477585 Msa0663570:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4_4:+2475798 Msa0663570:CDS
TACAGAAGATTCTTATCTTG+TGG 0.474072 4_4:-2475549 None:intergenic
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4_4:-2475381 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4_4:-2478495 Msa0663580:three_prime_UTR
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4_4:-2477596 Msa0663580:three_prime_UTR
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4_4:-2477601 Msa0663580:three_prime_UTR
AGAGAAGACTTACATAGTTA+GGG 0.493349 4_4:+2477933 Msa0663570:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4_4:-2475816 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4_4:+2476636 Msa0663570:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4_4:+2476661 Msa0663570:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4_4:+2477858 Msa0663570:intron
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4_4:+2478167 Msa0663570:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4_4:+2476689 Msa0663570:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4_4:+2477720 Msa0663570:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4_4:+2475479 Msa0663570:CDS
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4_4:-2475763 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4_4:+2478259 Msa0663570:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4_4:-2477511 Msa0663580:three_prime_UTR
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4_4:-2475879 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4_4:+2477534 Msa0663570:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4_4:+2478250 Msa0663570:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4_4:+2476012 Msa0663570:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4_4:+2478080 Msa0663570:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4_4:+2478377 Msa0663570:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4_4:+2475777 Msa0663570:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4_4:+2477291 Msa0663570:intron
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4_4:+2478146 Msa0663570:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4_4:+2477973 Msa0663570:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4_4:+2478000 Msa0663570:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4_4:+2476713 Msa0663570:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4_4:-2477919 Msa0663580:three_prime_UTR
TAGACACATACATAGTAGAC+AGG 0.544177 4_4:+2478215 Msa0663570:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4_4:+2477517 Msa0663570:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4_4:+2477721 Msa0663570:CDS
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4_4:-2478528 Msa0663580:three_prime_UTR
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4_4:+2478027 Msa0663570:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4_4:+2477586 Msa0663570:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4_4:+2475468 Msa0663570:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4_4:-2478529 Msa0663580:three_prime_UTR
TTTGACGAACTTTGAGGACC+CGG 0.557037 4_4:-2478402 Msa0663580:three_prime_UTR
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4_4:+2476662 Msa0663570:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4_4:-2478368 Msa0663580:three_prime_UTR
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4_4:+2477525 Msa0663570:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4_4:+2478491 Msa0663570:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4_4:-2475774 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4_4:+2477389 Msa0663570:CDS
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4_4:+2478012 Msa0663570:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4_4:+2475987 Msa0663570:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4_4:+2477292 Msa0663570:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4_4:+2475324 None:intergenic
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4_4:+2478317 Msa0663570:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4_4:+2477352 Msa0663570:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4_4:+2475480 Msa0663570:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4_4:+2478313 Msa0663570:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4_4:+2478286 Msa0663570:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4_4:+2478307 Msa0663570:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4_4:+2478350 Msa0663570:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4_4:-2477564 Msa0663580:three_prime_UTR
ATATACCAGGGTATGACAGT+TGG 0.606713 4_4:+2476726 Msa0663570:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4_4:+2475984 Msa0663570:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4_4:+2477320 Msa0663570:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4_4:+2478003 Msa0663570:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4_4:+2477505 Msa0663570:intron
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4_4:+2478320 Msa0663570:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4_4:-2477593 Msa0663580:three_prime_UTR
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4_4:+2477552 Msa0663570:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4_4:-2477328 Msa0663580:three_prime_UTR
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4_4:+2478073 Msa0663570:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4_4:+2478370 Msa0663570:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4_4:+2477937 Msa0663570:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4_4:+2477632 Msa0663570:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4_4:+2477618 Msa0663570:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4_4:-2477332 Msa0663580:three_prime_UTR
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4_4:+2475414 Msa0663570:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4_4:-2477862 Msa0663580:three_prime_UTR
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4_4:+2478371 Msa0663570:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4_4:-2476170 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4_4:-2476731 Msa0663580:three_prime_UTR
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4_4:+2475325 None:intergenic
ATAGACAGTAACAAAATTTG+GGG 0.668163 4_4:-2476871 Msa0663580:three_prime_UTR
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4_4:+2476600 Msa0663570:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4_4:+2477315 Msa0663570:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4_4:-2475760 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4_4:+2477633 Msa0663570:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4_4:+2478140 Msa0663570:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4_4:+2478260 Msa0663570:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4_4:+2478074 Msa0663570:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4_4:-2475755 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4_4:+2478351 Msa0663570:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4_4:-2477507 Msa0663580:three_prime_UTR
TCTATATTCAAGCTGCATGG+AGG 0.707083 4_4:+2478289 Msa0663570:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4_4:+2476015 Msa0663570:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4_4:+2476714 Msa0663570:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4_4:+2477634 Msa0663570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTAAAATTCTATAAATTA+TGG - chr4_4:2477139-2477158 Msa0663580:intron 10.0%
!!! AACTATTATTTTGTAGATAA+CGG + chr4_4:2476589-2476608 Msa0663570:intron 15.0%
!! AAATATATATGTATTAGTGT+CGG + chr4_4:2477017-2477036 Msa0663570:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG - chr4_4:2477188-2477207 Msa0663580:intron 15.0%
!!! ACTATTATTTTGTAGATAAC+GGG + chr4_4:2476590-2476609 Msa0663570:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG - chr4_4:2476980-2476999 Msa0663580:intron 20.0%
!! GAATCAAAGCATATTTATAA+AGG - chr4_4:2477198-2477217 Msa0663580:intron 20.0%
!! ATGTTTACAGTAAAAACTTT+CGG - chr4_4:2477419-2477438 Msa0663580:intron 20.0%
! AAGATAAGAATCTTCTGTAT+TGG + chr4_4:2475553-2475572 Msa0663570:CDS 25.0%
!!! GATATTTCATTATTGACTTC+TGG + chr4_4:2475847-2475866 Msa0663570:CDS 25.0%
! CAACAAACATTAAGACATAT+TGG - chr4_4:2475972-2475991 None:intergenic 25.0%
! TCTTAAGAAATGAACTTACA+TGG - chr4_4:2476207-2476226 None:intergenic 25.0%
! TATCATTCACATATTCTCTT+TGG + chr4_4:2476322-2476341 Msa0663570:intron 25.0%
!! ATAGAAAATTGGATGCTTTT+TGG - chr4_4:2476366-2476385 None:intergenic 25.0%
!!! AAAAAGCATCCAATTTTCTA+TGG + chr4_4:2476365-2476384 Msa0663570:intron 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG - chr4_4:2476397-2476416 None:intergenic 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG - chr4_4:2476398-2476417 None:intergenic 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG - chr4_4:2476438-2476457 None:intergenic 25.0%
! AATTCACATTTCTTGTATTG+TGG + chr4_4:2476689-2476708 Msa0663570:CDS 25.0%
! ATAGACAGTAACAAAATTTG+GGG - chr4_4:2476874-2476893 Msa0663580:three_prime_UTR 25.0%
! CATAGACAGTAACAAAATTT+GGG - chr4_4:2476875-2476894 Msa0663580:three_prime_UTR 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG - chr4_4:2476967-2476986 Msa0663580:intron 25.0%
! TTCCATAGTAATAGCTATTA+TGG + chr4_4:2477350-2477369 Msa0663570:CDS 25.0%
! TCCATAGTAATAGCTATTAT+GGG + chr4_4:2477351-2477370 Msa0663570:CDS 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG - chr4_4:2477689-2477708 Msa0663580:three_prime_UTR 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG + chr4_4:2477720-2477739 Msa0663570:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG + chr4_4:2477721-2477740 Msa0663570:CDS 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG - chr4_4:2477807-2477826 Msa0663580:intron 25.0%
! GTGTATGACATATATTATTC+AGG + chr4_4:2477837-2477856 Msa0663570:intron 25.0%
!! TCAGATGGACTAATGAATAT+TGG + chr4_4:2475450-2475469 Msa0663570:CDS 30.0%
TACAGAAGATTCTTATCTTG+TGG - chr4_4:2475552-2475571 None:intergenic 30.0%
TACGGAAGATAGAAAAAGAA+AGG - chr4_4:2475624-2475643 None:intergenic 30.0%
TACGATTTGTGGTTGAAATA+CGG - chr4_4:2475642-2475661 None:intergenic 30.0%
! AATATTTTCCGAGAATGGTA+CGG + chr4_4:2476080-2476099 Msa0663570:CDS 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG - chr4_4:2476112-2476131 None:intergenic 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG + chr4_4:2476145-2476164 Msa0663570:CDS 30.0%
ATACCAGATCAAATAGTACA+TGG - chr4_4:2476173-2476192 None:intergenic 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG + chr4_4:2476282-2476301 Msa0663570:intron 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG - chr4_4:2476287-2476306 None:intergenic 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG - chr4_4:2476437-2476456 None:intergenic 30.0%
TTGTAGATATTCTGACATAG+AGG + chr4_4:2476493-2476512 Msa0663570:intron 30.0%
GTTAGAATAGATTACGCTAT+AGG + chr4_4:2476515-2476534 Msa0663570:intron 30.0%
AAAGCATGTAAAGAAGTTGA+GGG + chr4_4:2476662-2476681 Msa0663570:CDS 30.0%
!! GATAACTCAGCTTTTAACTA+TGG + chr4_4:2476797-2476816 Msa0663570:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG - chr4_4:2476861-2476880 Msa0663580:three_prime_UTR 30.0%
CCATAGACAGTAACAAAATT+TGG - chr4_4:2476876-2476895 Msa0663580:three_prime_UTR 30.0%
! CCAAATTTTGTTACTGTCTA+TGG + chr4_4:2476873-2476892 Msa0663570:CDS 30.0%
! AATTTTGTTACTGTCTATGG+TGG + chr4_4:2476876-2476895 Msa0663570:CDS 30.0%
! ATTTTGTTACTGTCTATGGT+GGG + chr4_4:2476877-2476896 Msa0663570:CDS 30.0%
ATGGCTAATGTGAAAAACAT+AGG - chr4_4:2477046-2477065 Msa0663580:intron 30.0%
TCTGATCTTCATATCAAAGT+CGG + chr4_4:2477094-2477113 Msa0663570:intron 30.0%
ATTGTAGATTCACTTGATGT+TGG + chr4_4:2477157-2477176 Msa0663570:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG + chr4_4:2477158-2477177 Msa0663570:intron 30.0%
AAGCAAAACAGAAGGATATT+TGG - chr4_4:2477272-2477291 Msa0663580:intron 30.0%
CCATAGTAATAGCTATTATG+GGG + chr4_4:2477352-2477371 Msa0663570:CDS 30.0%
CCAATTGCATTTATAACACA+AGG - chr4_4:2477482-2477501 Msa0663580:intron 30.0%
CCTTGTGTTATAAATGCAAT+TGG + chr4_4:2477479-2477498 Msa0663570:intron 30.0%
GTCTTCTCTGTATATAAACT+CGG - chr4_4:2477922-2477941 Msa0663580:three_prime_UTR 30.0%
AGAGAAGACTTACATAGTTA+GGG + chr4_4:2477933-2477952 Msa0663570:CDS 30.0%
ATATTACGAATCTGATTACC+CGG + chr4_4:2478350-2478369 Msa0663570:CDS 30.0%
ATTGGAAGATCACTCAAAAG+TGG + chr4_4:2475468-2475487 Msa0663570:CDS 35.0%
!! TGTGACACTTTAAGATCTTC+TGG - chr4_4:2475513-2475532 None:intergenic 35.0%
TGGCTAAACAATACGATTTG+TGG - chr4_4:2475653-2475672 None:intergenic 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG - chr4_4:2475673-2475692 None:intergenic 35.0%
CAAAACATATTGAGCATGGA+TGG - chr4_4:2475904-2475923 None:intergenic 35.0%
TACGCAAAACATATTGAGCA+TGG - chr4_4:2475908-2475927 None:intergenic 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG + chr4_4:2475984-2476003 Msa0663570:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG + chr4_4:2476075-2476094 Msa0663570:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG + chr4_4:2476118-2476137 Msa0663570:CDS 35.0%
CATAAGTGATACAGAAGCAA+TGG - chr4_4:2476251-2476270 None:intergenic 35.0%
TCTGTATCACTTATGAAGCT+CGG + chr4_4:2476256-2476275 Msa0663570:intron 35.0%
GGGAAGTTTCCATAGAAAAT+TGG - chr4_4:2476377-2476396 None:intergenic 35.0%
! GAAGTATCAAGAAGTGTATG+AGG - chr4_4:2476415-2476434 None:intergenic 35.0%
AGAATAGATTACGCTATAGG+CGG + chr4_4:2476518-2476537 Msa0663570:intron 35.0%
GAAACATTGATCATTCGTGT+CGG - chr4_4:2476564-2476583 None:intergenic 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG + chr4_4:2476661-2476680 Msa0663570:CDS 35.0%
AGTTCAAGTAAGCATATACC+AGG + chr4_4:2476713-2476732 Msa0663570:CDS 35.0%
GTTCAAGTAAGCATATACCA+GGG + chr4_4:2476714-2476733 Msa0663570:CDS 35.0%
! TTTTGTTACTGTCTATGGTG+GGG + chr4_4:2476878-2476897 Msa0663570:CDS 35.0%
TGTTATAAAGAGGACTCATG+TGG - chr4_4:2476957-2476976 Msa0663580:intron 35.0%
! TTTTCACATTAGCCATGTAG+AGG + chr4_4:2477050-2477069 Msa0663570:intron 35.0%
ACTGTGAAAAGCAAAACAGA+AGG - chr4_4:2477280-2477299 Msa0663580:intron 35.0%
!! TTTGCTTTTCACAGTACACT+TGG + chr4_4:2477285-2477304 Msa0663570:intron 35.0%
CCCCATAATAGCTATTACTA+TGG - chr4_4:2477355-2477374 Msa0663580:three_prime_UTR 35.0%
TGCAATGAGAAATCTCAGAA+AGG - chr4_4:2477771-2477790 Msa0663580:intron 35.0%
CAGAGAAGACTTACATAGTT+AGG + chr4_4:2477932-2477951 Msa0663570:CDS 35.0%
AAGACTTACATAGTTAGGGA+AGG + chr4_4:2477937-2477956 Msa0663570:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG - chr4_4:2477967-2477986 Msa0663580:three_prime_UTR 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG + chr4_4:2478054-2478073 Msa0663570:CDS 35.0%
TAGACACATACATAGTAGAC+AGG + chr4_4:2478215-2478234 Msa0663570:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG - chr4_4:2478254-2478273 Msa0663580:three_prime_UTR 35.0%
CAATCTATATTCAAGCTGCA+TGG + chr4_4:2478286-2478305 Msa0663570:CDS 35.0%
TATTACGAATCTGATTACCC+GGG + chr4_4:2478351-2478370 Msa0663570:CDS 35.0%
!! CCAAATTTTGACGAACTTTG+AGG - chr4_4:2478411-2478430 Msa0663580:three_prime_UTR 35.0%
CCTCAAAGTTCGTCAAAATT+TGG + chr4_4:2478408-2478427 Msa0663570:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG + chr4_4:2478436-2478455 Msa0663570:CDS 35.0%
! TGTTTGCCTTTTCCTATTCC+CGG + chr4_4:2475375-2475394 Msa0663570:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG - chr4_4:2475401-2475420 None:intergenic 40.0%
! TCGTCGAAATCATTTCGAGT+CGG + chr4_4:2475414-2475433 Msa0663570:CDS 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG + chr4_4:2475425-2475444 Msa0663570:CDS 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG + chr4_4:2475426-2475445 Msa0663570:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG + chr4_4:2475479-2475498 Msa0663570:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG - chr4_4:2475777-2475796 None:intergenic 40.0%
TCATCTCGAGTATTTGGAAG+AGG + chr4_4:2475777-2475796 Msa0663570:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG + chr4_4:2475987-2476006 Msa0663570:intron 40.0%
AATGGTACGGTTCATACCTT+TGG + chr4_4:2476093-2476112 Msa0663570:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG + chr4_4:2476167-2476186 Msa0663570:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG + chr4_4:2476420-2476439 Msa0663570:intron 40.0%
ATATACCAGGGTATGACAGT+TGG + chr4_4:2476726-2476745 Msa0663570:CDS 40.0%
GAAGATCAGACACACGATAT+GGG - chr4_4:2477084-2477103 Msa0663580:intron 40.0%
TGAAGATCAGACACACGATA+TGG - chr4_4:2477085-2477104 Msa0663580:intron 40.0%
! TTTTCACAGTACACTTGGTC+AGG + chr4_4:2477290-2477309 Msa0663570:intron 40.0%
TTTCACAGTACACTTGGTCA+GGG + chr4_4:2477291-2477310 Msa0663570:intron 40.0%
TGGAAAACAAAACCTCTCCA+GGG - chr4_4:2477335-2477354 Msa0663580:three_prime_UTR 40.0%
ATGGAAAACAAAACCTCTCC+AGG - chr4_4:2477336-2477355 Msa0663580:three_prime_UTR 40.0%
TCTTCTGATTGGAAACATGC+AGG + chr4_4:2477389-2477408 Msa0663570:CDS 40.0%
GACCTACCTTCAATCAATGT+CGG + chr4_4:2477505-2477524 Msa0663570:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG - chr4_4:2477510-2477529 Msa0663580:three_prime_UTR 40.0%
TGGAGGAAATGAGGATCAAA+AGG + chr4_4:2477534-2477553 Msa0663570:CDS 40.0%
! AATTTTCTTCATCGACCCCT+CGG - chr4_4:2477652-2477671 Msa0663580:three_prime_UTR 40.0%
AGGGGTCGATGAAGAAAATT+TGG + chr4_4:2477652-2477671 Msa0663570:CDS 40.0%
GAGATGTTGTTCATCATTCG+TGG + chr4_4:2477973-2477992 Msa0663570:CDS 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG + chr4_4:2478000-2478019 Msa0663570:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG + chr4_4:2478038-2478057 Msa0663570:CDS 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG + chr4_4:2478042-2478061 Msa0663570:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG + chr4_4:2478074-2478093 Msa0663570:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG - chr4_4:2478129-2478148 Msa0663580:three_prime_UTR 40.0%
TACCTCACTCATAGCTTTCA+CGG - chr4_4:2478145-2478164 Msa0663580:three_prime_UTR 40.0%
TCCGTTTCTATTCGCAACAA+TGG + chr4_4:2478250-2478269 Msa0663570:CDS 40.0%
ATTCGCAACAATGGAGAACA+TGG + chr4_4:2478259-2478278 Msa0663570:CDS 40.0%
TTCGCAACAATGGAGAACAT+GGG + chr4_4:2478260-2478279 Msa0663570:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG + chr4_4:2478289-2478308 Msa0663570:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG - chr4_4:2478540-2478559 Msa0663580:three_prime_UTR 40.0%
TTCATCCCACTTGAGCTAGA+TGG - chr4_4:2475333-2475352 None:intergenic 45.0%
GGAGTGAAAAAGGGATCAGA+TGG + chr4_4:2475435-2475454 Msa0663570:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG + chr4_4:2475478-2475497 Msa0663570:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG + chr4_4:2475480-2475499 Msa0663570:CDS 45.0%
! AGATGAAGGTGCAATGAAGG+CGG - chr4_4:2475763-2475782 None:intergenic 45.0%
! TCGAGATGAAGGTGCAATGA+AGG - chr4_4:2475766-2475785 None:intergenic 45.0%
GCACCTTCATCTCGAGTATT+TGG + chr4_4:2475771-2475790 Msa0663570:CDS 45.0%
GGTGAACTTGTCATAGATCC+TGG + chr4_4:2475798-2475817 Msa0663570:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG - chr4_4:2475819-2475838 None:intergenic 45.0%
TGGATGGTATCTCAAACCTG+TGG - chr4_4:2475888-2475907 None:intergenic 45.0%
TCTTTATAGCGCAAGACGCA+TGG + chr4_4:2476012-2476031 Msa0663570:CDS 45.0%
GGTATGAACCGTACCATTCT+CGG - chr4_4:2476091-2476110 None:intergenic 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG + chr4_4:2476600-2476619 Msa0663570:intron 45.0%
TAGAACGTAACGACACATGC+TGG + chr4_4:2476636-2476655 Msa0663570:CDS 45.0%
TTTCTCCAACTGTCATACCC+TGG - chr4_4:2476734-2476753 Msa0663580:three_prime_UTR 45.0%
GGTGGGGACTTCAGAATTTA+AGG + chr4_4:2476894-2476913 Msa0663570:CDS 45.0%
TTCACAGTACACTTGGTCAG+GGG + chr4_4:2477292-2477311 Msa0663570:intron 45.0%
AAACAAAACCTCTCCAGGGT+AGG - chr4_4:2477331-2477350 Msa0663580:three_prime_UTR 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG + chr4_4:2477378-2477397 Msa0663570:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG - chr4_4:2477514-2477533 Msa0663580:three_prime_UTR 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG + chr4_4:2477514-2477533 Msa0663570:CDS 45.0%
GGGTGTTCAACCACTTGTAT+TGG - chr4_4:2477631-2477650 Msa0663580:three_prime_UTR 45.0%
! ATTTTCTTCATCGACCCCTC+GGG - chr4_4:2477651-2477670 Msa0663580:three_prime_UTR 45.0%
GGTTCCTCTCTTTGCTAACA+TGG + chr4_4:2477858-2477877 Msa0663570:intron 45.0%
TCGTCCATGTTAGCAAAGAG+AGG - chr4_4:2477865-2477884 Msa0663580:three_prime_UTR 45.0%
!! GAGAGTGTTACTACAGATGG+TGG + chr4_4:2478003-2478022 Msa0663570:CDS 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG + chr4_4:2478012-2478031 Msa0663570:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG + chr4_4:2478027-2478046 Msa0663570:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG + chr4_4:2478073-2478092 Msa0663570:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG + chr4_4:2478080-2478099 Msa0663570:CDS 45.0%
AACCGTGAAAGCTATGAGTG+AGG + chr4_4:2478140-2478159 Msa0663570:CDS 45.0%
GAAAGCTATGAGTGAGGTAG+AGG + chr4_4:2478146-2478165 Msa0663570:CDS 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG + chr4_4:2478167-2478186 Msa0663570:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG + chr4_4:2478301-2478320 Msa0663570:CDS 45.0%
TTGCAGAATCATCACTTCCC+GGG - chr4_4:2478371-2478390 Msa0663580:three_prime_UTR 45.0%
CTTGCAGAATCATCACTTCC+CGG - chr4_4:2478372-2478391 Msa0663580:three_prime_UTR 45.0%
GGGAAGTGATGATTCTGCAA+GGG + chr4_4:2478371-2478390 Msa0663570:CDS 45.0%
! TTTGACGAACTTTGAGGACC+CGG - chr4_4:2478405-2478424 Msa0663580:three_prime_UTR 45.0%
AGTACCAAGTTCAAGAGAGC+TGG + chr4_4:2478491-2478510 Msa0663570:CDS 45.0%
TTCACCAGCTCTCTTGAACT+TGG - chr4_4:2478498-2478517 Msa0663580:three_prime_UTR 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG - chr4_4:2478524-2478543 Msa0663580:three_prime_UTR 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG - chr4_4:2478525-2478544 Msa0663580:three_prime_UTR 45.0%
! GCACTGAAATCAGGTTCTGA+GGG - chr4_4:2478531-2478550 Msa0663580:three_prime_UTR 45.0%
! AGCACTGAAATCAGGTTCTG+AGG - chr4_4:2478532-2478551 Msa0663580:three_prime_UTR 45.0%
!!! AATTGAATATAAATAAATTT+TGG - chr4_4:2476449-2476468 None:intergenic 5.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG - chr4_4:2475396-2475415 None:intergenic 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG - chr4_4:2475397-2475416 None:intergenic 50.0%
GTATCTCAAACCTGTGGCAG+TGG - chr4_4:2475882-2475901 None:intergenic 50.0%
TTATAGCGCAAGACGCATGG+AGG + chr4_4:2476015-2476034 Msa0663570:CDS 50.0%
CTTCTGACTAGTACCTACCC+TGG + chr4_4:2477315-2477334 Msa0663570:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG + chr4_4:2477517-2477536 Msa0663570:CDS 50.0%
CTGACTCTTTCCCTTAGGTC+CGG - chr4_4:2477599-2477618 Msa0663580:three_prime_UTR 50.0%
AGCGTCTGACTCTTTCCCTT+AGG - chr4_4:2477604-2477623 Msa0663580:three_prime_UTR 50.0%
GACGCTACGACCAATACAAG+TGG + chr4_4:2477618-2477637 Msa0663570:CDS 50.0%
TACAAGTGGTTGAACACCCG+AGG + chr4_4:2477632-2477651 Msa0663570:CDS 50.0%
ACAAGTGGTTGAACACCCGA+GGG + chr4_4:2477633-2477652 Msa0663570:CDS 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG - chr4_4:2478108-2478127 Msa0663580:three_prime_UTR 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG + chr4_4:2478307-2478326 Msa0663570:CDS 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG + chr4_4:2478313-2478332 Msa0663570:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG + chr4_4:2478317-2478336 Msa0663570:CDS 50.0%
CGGGAAGTGATGATTCTGCA+AGG + chr4_4:2478370-2478389 Msa0663570:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG + chr4_4:2478377-2478396 Msa0663570:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG + chr4_4:2478456-2478475 Msa0663570:CDS 50.0%
CAATGAAGGCGGTGCGGAAT+TGG - chr4_4:2475752-2475771 None:intergenic 55.0%
AAGGTGCAATGAAGGCGGTG+CGG - chr4_4:2475758-2475777 None:intergenic 55.0%
! CGCAAGACGCATGGAGGTAT+TGG + chr4_4:2476021-2476040 Msa0663570:CDS 55.0%
TGGGCACATGCACCTCTACA+TGG - chr4_4:2477065-2477084 Msa0663580:intron 55.0%
GACTAGTACCTACCCTGGAG+AGG + chr4_4:2477320-2477339 Msa0663570:CDS 55.0%
CGGTTCGTCTGGAGGAAATG+AGG + chr4_4:2477525-2477544 Msa0663570:CDS 55.0%
GCGATGATGCATCCACTGCT+CGG - chr4_4:2477567-2477586 Msa0663580:three_prime_UTR 55.0%
GCATCATCGCTTGCTTCCTC+CGG + chr4_4:2477577-2477596 Msa0663570:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG + chr4_4:2477586-2477605 Msa0663570:CDS 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG - chr4_4:2477596-2477615 Msa0663580:three_prime_UTR 55.0%
CAAGTGGTTGAACACCCGAG+GGG + chr4_4:2477634-2477653 Msa0663570:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG + chr4_4:2478383-2478402 Msa0663570:CDS 55.0%
! CCTTTTCCTATTCCCGGCCG+CGG + chr4_4:2475381-2475400 Msa0663570:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG + chr4_4:2475382-2475401 Msa0663570:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG - chr4_4:2475390-2475409 None:intergenic 60.0%
GTCTGTACTGCCACTGCCAC+AGG + chr4_4:2475869-2475888 Msa0663570:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG + chr4_4:2477552-2477571 Msa0663570:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG + chr4_4:2477585-2477604 Msa0663570:CDS 60.0%
AAGGCGAAGGCGAAGGAAGG+AGG + chr4_4:2478320-2478339 Msa0663570:CDS 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG + chr4_4:2478384-2478403 Msa0663570:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG - chr4_4:2475384-2475403 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 2475327 2478569 2475327 ID=Msa0663570;Name=Msa0663570
chr4_4 mRNA 2475327 2478569 2475327 ID=Msa0663570-mRNA-1;Parent=Msa0663570;Name=Msa0663570-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|6|0|715
chr4_4 exon 2475327 2475890 2475327 ID=Msa0663570-mRNA-1:exon:640;Parent=Msa0663570-mRNA-1
chr4_4 exon 2475995 2476207 2475995 ID=Msa0663570-mRNA-1:exon:641;Parent=Msa0663570-mRNA-1
chr4_4 exon 2476605 2476915 2476605 ID=Msa0663570-mRNA-1:exon:642;Parent=Msa0663570-mRNA-1
chr4_4 exon 2477299 2477410 2477299 ID=Msa0663570-mRNA-1:exon:643;Parent=Msa0663570-mRNA-1
chr4_4 exon 2477506 2477742 2477506 ID=Msa0663570-mRNA-1:exon:644;Parent=Msa0663570-mRNA-1
chr4_4 exon 2477859 2478569 2477859 ID=Msa0663570-mRNA-1:exon:645;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2475327 2475890 2475327 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2475995 2476207 2475995 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2476605 2476915 2476605 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2477299 2477410 2477299 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2477506 2477742 2477506 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
chr4_4 CDS 2477859 2478569 2477859 ID=Msa0663570-mRNA-1:cds;Parent=Msa0663570-mRNA-1
Gene Sequence

>Msa0663570

ATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCACAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>Msa0663570

MPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE*