AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585710 MtrunA17_Chr4g0001481 97.177 744 20 1 1 744 1 743 0.0 1498
Msa0585710 MtrunA17_Chr4g0073941 64.793 747 235 11 1 739 1 727 0.0 951
Msa0585710 MtrunA17_Chr1g0158861 49.732 746 326 12 14 744 5 716 0.0 715
Msa0585710 MtrunA17_Chr3g0126541 50.270 740 321 9 14 739 4 710 0.0 713
Msa0585710 MtrunA17_Chr2g0305771 52.199 682 293 6 82 744 24 691 0.0 708
Msa0585710 MtrunA17_Chr3g0139071 52.246 668 295 7 89 739 61 721 0.0 677
Msa0585710 MtrunA17_Chr2g0326871 49.517 725 327 14 20 738 10 701 0.0 677
Msa0585710 MtrunA17_Chr7g0275581 48.780 738 334 10 17 739 7 715 0.0 666
Msa0585710 MtrunA17_Chr4g0028861 55.150 602 257 7 69 666 90 682 0.0 664
Msa0585710 MtrunA17_Chr1g0181121 51.420 669 293 13 89 742 32 683 0.0 656
Msa0585710 MtrunA17_Chr7g0228181 51.156 649 294 10 100 738 43 678 0.0 655
Msa0585710 MtrunA17_Chr8g0347121 45.407 762 336 11 14 737 4 723 0.0 630
Msa0585710 MtrunA17_Chr3g0125651 47.661 684 317 12 90 743 20 692 0.0 630
Msa0585710 MtrunA17_Chr6g0481021 40.956 586 310 15 101 666 123 692 8.42e-128 396
Msa0585710 MtrunA17_Chr6g0480851 38.889 594 327 14 101 678 184 757 6.77e-121 380
Msa0585710 MtrunA17_Chr6g0480671 39.626 588 324 12 100 672 192 763 8.79e-121 380
Msa0585710 MtrunA17_Chr6g0480781 34.429 700 400 18 16 686 90 759 3.94e-117 370
Msa0585710 MtrunA17_Chr8g0360821 35.777 573 348 10 103 664 64 627 6.65e-110 348
Msa0585710 MtrunA17_Chr7g0218341 36.333 589 354 9 86 666 102 677 4.37e-109 347
Msa0585710 MtrunA17_Chr6g0480741 35.495 586 335 15 100 664 114 677 1.96e-97 316
Msa0585710 MtrunA17_Chr6g0480681 33.750 640 349 19 101 689 115 730 1.89e-91 302
Msa0585710 MtrunA17_Chr2g0318561 36.245 458 273 9 217 664 2 450 5.78e-91 293
Msa0585710 MtrunA17_Chr5g0395421 38.162 359 186 11 299 642 18 355 2.32e-60 208
Msa0585710 MtrunA17_Chr6g0480771 39.113 248 128 6 425 659 12 249 3.62e-42 155
Msa0585710 MtrunA17_Chr2g0326861 40.000 205 114 4 176 378 14 211 6.38e-40 146
Msa0585710 MtrunA17_Chr6g0480841 41.237 194 94 5 479 659 2 188 1.52e-34 131
Msa0585710 MtrunA17_Chr5g0395411 35.821 201 114 4 101 300 172 358 2.03e-32 129
Msa0585710 MtrunA17_Chr2g0326851 43.262 141 62 5 604 738 3 131 2.59e-27 107
Msa0585710 MtrunA17_Chr6g0480751 36.567 134 85 0 100 233 97 230 2.56e-23 99.4
Msa0585710 MtrunA17_Chr6g0480791 41.176 119 69 1 101 218 172 290 7.06e-22 96.7
Msa0585710 MtrunA17_Chr4g0063141 25.366 205 150 2 379 582 239 441 3.81e-17 86.3
Msa0585710 MtrunA17_Chr3g0138671 25.616 203 148 2 380 582 243 442 2.61e-16 83.2
Msa0585710 MtrunA17_Chr2g0277651 24.020 204 152 2 379 582 262 462 5.63e-15 79.0
Msa0585710 MtrunA17_Chr5g0434371 24.194 186 138 2 374 559 277 459 1.12e-12 71.6
Msa0585710 MtrunA17_Chr6g0480761 28.859 149 97 5 243 389 24 165 2.22e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585710 AT1G19780.1 68.519 756 215 10 6 744 2 751 0.0 1024
Msa0585710 AT1G15990.1 68.133 750 209 14 6 739 2 737 0.0 999
Msa0585710 AT2G23980.9 62.914 755 253 10 1 739 1 744 0.0 955
Msa0585710 AT2G23980.10 62.914 755 253 10 1 739 1 744 0.0 955
Msa0585710 AT2G23980.1 62.914 755 253 10 1 739 1 744 0.0 955
Msa0585710 AT2G23980.4 62.914 755 253 10 1 739 1 744 0.0 955
Msa0585710 AT2G23980.5 62.914 755 253 10 1 739 1 744 0.0 955
Msa0585710 AT4G30560.1 63.431 752 239 9 1 739 1 729 0.0 949
Msa0585710 AT4G30560.4 63.431 752 239 9 1 739 1 729 0.0 949
Msa0585710 AT5G57940.1 64.441 734 238 8 11 739 1 716 0.0 943
Msa0585710 AT5G57940.2 64.441 734 238 8 11 739 1 716 0.0 943
Msa0585710 AT2G23980.3 61.054 778 253 11 1 739 1 767 0.0 941
Msa0585710 AT2G23980.2 61.054 778 253 11 1 739 1 767 0.0 941
Msa0585710 AT2G23980.8 61.054 778 253 11 1 739 1 767 0.0 941
Msa0585710 AT2G23980.7 61.054 778 253 11 1 739 1 767 0.0 941
Msa0585710 AT5G57940.3 63.896 734 235 8 11 739 1 709 0.0 930
Msa0585710 AT2G23980.6 63.049 728 246 9 24 739 1 717 0.0 928
Msa0585710 AT4G30560.5 63.636 726 231 8 24 739 1 703 0.0 924
Msa0585710 AT4G30560.2 63.636 726 231 8 24 739 1 703 0.0 924
Msa0585710 AT4G30560.3 63.636 726 231 8 24 739 1 703 0.0 924
Msa0585710 AT1G19780.2 70.425 541 145 7 217 744 2 540 0.0 754
Msa0585710 AT1G01340.2 53.707 661 279 6 92 739 59 705 0.0 718
Msa0585710 AT1G01340.1 53.707 661 279 6 92 739 54 700 0.0 717
Msa0585710 AT4G01010.1 51.489 705 293 11 53 741 24 695 0.0 704
Msa0585710 AT4G01010.2 51.489 705 293 11 53 741 24 695 0.0 704
Msa0585710 AT5G53130.1 49.730 742 326 10 14 739 4 714 0.0 692
Msa0585710 AT2G28260.1 50.946 687 305 8 55 739 21 677 0.0 683
Msa0585710 AT2G24610.1 52.444 675 287 11 89 739 60 724 0.0 682
Msa0585710 AT2G24610.2 52.444 675 287 11 89 739 75 739 0.0 681
Msa0585710 AT2G28260.2 52.024 667 289 7 74 739 1 637 0.0 680
Msa0585710 AT4G30360.1 51.672 658 299 10 99 743 69 720 0.0 655
Msa0585710 AT2G46430.1 47.241 743 327 12 14 737 5 701 0.0 653
Msa0585710 AT2G46430.2 47.241 743 327 12 14 737 5 701 0.0 653
Msa0585710 AT5G14870.1 49.552 670 303 9 98 738 37 700 0.0 647
Msa0585710 AT3G48010.1 47.640 678 303 12 98 737 41 704 0.0 623
Msa0585710 AT2G46440.1 43.339 623 309 13 99 706 28 621 4.09e-162 483
Msa0585710 AT2G46450.4 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0585710 AT2G46450.1 38.473 655 342 16 99 735 28 639 1.42e-148 449
Msa0585710 AT2G46450.3 37.557 655 325 17 99 735 15 603 1.43e-140 427
Msa0585710 AT2G46450.2 37.557 655 325 17 99 735 28 616 1.93e-140 427
Msa0585710 AT2G46440.2 47.097 465 208 10 256 706 3 443 3.46e-130 394
Msa0585710 AT3G17690.1 37.563 591 338 14 90 667 147 719 4.33e-119 375
Msa0585710 AT3G17700.1 37.544 570 328 10 101 658 192 745 3.87e-115 365
Msa0585710 AT5G15410.1 36.678 578 347 9 94 658 106 677 2.88e-108 346
Msa0585710 AT5G15410.2 36.678 578 347 9 94 658 105 676 3.08e-108 346
Msa0585710 AT5G54250.2 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0585710 AT5G54250.1 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0585710 AT5G54250.4 34.549 576 361 8 100 664 78 648 1.46e-107 343
Msa0585710 AT3G17690.2 37.897 504 291 10 90 589 147 632 1.03e-105 337
Msa0585710 AT3G17700.2 37.576 495 292 7 101 594 192 670 5.16e-103 331
Msa0585710 AT5G54250.3 33.854 576 360 9 100 664 78 643 4.18e-102 329
Msa0585710 AT3G17690.3 35.433 381 231 8 90 466 147 516 2.86e-66 228
Msa0585710 AT4G22200.2 24.020 204 152 2 379 582 263 463 2.29e-16 83.6
Msa0585710 AT4G22200.1 24.020 204 152 2 379 582 263 463 5.32e-16 82.8
Msa0585710 AT5G37500.1 21.702 470 273 13 99 561 55 436 8.12e-15 79.0
Msa0585710 AT5G37500.2 21.489 470 274 13 99 561 55 436 1.01e-14 79.0
Msa0585710 AT5G37500.4 20.726 468 280 12 99 561 55 436 1.32e-14 78.2
Msa0585710 AT5G37500.3 20.833 408 252 9 155 561 45 382 3.13e-14 77.0
Msa0585710 AT2G26650.1 23.902 205 153 2 379 582 239 441 3.88e-14 77.0
Msa0585710 AT2G26650.2 23.902 205 153 2 379 582 134 336 5.30e-14 76.3
Msa0585710 AT5G46240.1 22.727 198 152 1 379 576 246 442 5.62e-14 76.3
Msa0585710 AT3G02850.2 20.902 488 293 14 83 563 56 457 1.04e-13 75.5
Msa0585710 AT3G02850.1 20.902 488 293 14 83 563 56 457 1.27e-13 75.1
Msa0585710 AT3G02850.3 20.697 488 294 14 83 563 56 457 1.58e-13 74.7
Msa0585710 AT4G32650.2 23.153 203 153 2 380 582 276 475 1.58e-12 71.2
Msa0585710 AT4G32650.3 23.153 203 153 2 380 582 276 475 2.14e-12 71.2
Msa0585710 AT4G32650.1 23.153 203 153 2 380 582 276 475 2.27e-12 70.9

Find 148 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4_2:+6794831 Msa0585710:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4_2:-6795586 Msa0585720:three_prime_UTR
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4_2:+6794375 Msa0585710:CDS
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4_2:+6795889 Msa0585710:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4_2:+6793252 Msa0585710:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4_2:-6794354 Msa0585720:three_prime_UTR
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4_2:+6795864 Msa0585710:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4_2:+6795782 Msa0585710:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4_2:-6792991 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4_2:-6795167 Msa0585720:three_prime_UTR
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4_2:+6792906 Msa0585710:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4_2:-6793151 None:intergenic
ATGATGTAGGTTGGATGATT+TGG 0.281948 4_2:+6792771 Msa0585710:intron
CAGAGAAGACTTACATAGTT+AGG 0.283571 4_2:+6795413 Msa0585710:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4_2:+6795133 Msa0585710:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4_2:-6794833 Msa0585720:three_prime_UTR
GATATTTCATTATTGACTTC+TGG 0.297261 4_2:+6793328 Msa0585710:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4_2:+6795865 Msa0585710:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4_2:-6794339 Msa0585720:three_prime_UTR
CATAGACAGTAACAAAATTT+GGG 0.315147 4_2:-6794353 Msa0585720:three_prime_UTR
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4_2:+6794859 Msa0585710:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4_2:-6793230 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4_2:+6794832 Msa0585710:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4_2:-6796002 Msa0585720:three_prime_UTR
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4_2:+6793501 Msa0585710:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4_2:-6796003 Msa0585720:three_prime_UTR
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4_2:-6792811 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4_2:+6792931 Msa0585710:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4_2:+6794995 Msa0585710:CDS
GCTAGATGGCATTGACGAAT+TGG 0.374592 4_2:-6792797 None:intergenic
AATGGTACGGTTCATACCTT+TGG 0.384471 4_2:+6793573 Msa0585710:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4_2:+6795917 Msa0585710:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4_2:-6796018 Msa0585720:three_prime_UTR
ATGAACAACATCTCATCAAC+TGG 0.395916 4_2:-6795445 Msa0585720:three_prime_UTR
AAGATAAGAATCTTCTGTAT+TGG 0.412931 4_2:+6793034 Msa0585710:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4_2:-6795850 Msa0585720:three_prime_UTR
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4_2:+6795937 Msa0585710:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4_2:-6795607 Msa0585720:three_prime_UTR
TACCTCACTCATAGCTTTCA+CGG 0.425446 4_2:-6795623 Msa0585720:three_prime_UTR
TACGATTTGTGGTTGAAATA+CGG 0.428941 4_2:-6793120 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4_2:+6793350 Msa0585710:CDS
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4_2:+6792959 Msa0585710:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4_2:-6795732 Msa0585720:three_prime_UTR
GTGCCATGTACTATTTGATC+TGG 0.449942 4_2:+6793647 Msa0585710:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4_2:-6795109 Msa0585720:three_prime_UTR
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4_2:-6794814 Msa0585720:three_prime_UTR
TGGCTAAACAATACGATTTG+TGG 0.457799 4_2:-6793131 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4_2:-6793568 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4_2:+6795058 Msa0585710:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4_2:+6795066 Msa0585710:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4_2:+6793279 Msa0585710:CDS
TACAGAAGATTCTTATCTTG+TGG 0.474072 4_2:-6793030 None:intergenic
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4_2:-6792862 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4_2:-6795976 Msa0585720:three_prime_UTR
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4_2:-6795077 Msa0585720:three_prime_UTR
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4_2:-6795082 Msa0585720:three_prime_UTR
TTCTTCACAATGTTTGACTC+TGG 0.491771 4_2:+6792320 None:intergenic
AGAGAAGACTTACATAGTTA+GGG 0.493349 4_2:+6795414 Msa0585710:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4_2:-6793297 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4_2:+6794117 Msa0585710:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4_2:+6794142 Msa0585710:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4_2:+6795339 Msa0585710:intron
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4_2:+6795648 Msa0585710:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4_2:+6794170 Msa0585710:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4_2:+6795201 Msa0585710:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4_2:+6792960 Msa0585710:CDS
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4_2:-6793244 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4_2:+6795740 Msa0585710:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4_2:-6794992 Msa0585720:three_prime_UTR
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4_2:-6793360 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4_2:+6795015 Msa0585710:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4_2:+6795731 Msa0585710:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4_2:+6793492 Msa0585710:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4_2:+6795561 Msa0585710:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4_2:+6795858 Msa0585710:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4_2:+6793258 Msa0585710:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4_2:+6794772 Msa0585710:intron
TACAAGTCACAGTATATTGA+AGG 0.537328 4_2:+6792344 Msa0585710:CDS
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4_2:+6795627 Msa0585710:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4_2:+6795454 Msa0585710:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4_2:+6795481 Msa0585710:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4_2:+6794194 Msa0585710:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4_2:-6795400 Msa0585720:three_prime_UTR
TAGACACATACATAGTAGAC+AGG 0.544177 4_2:+6795696 Msa0585710:CDS
ACAGTATATTGAAGGTCAGA+AGG 0.547235 4_2:+6792352 Msa0585710:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4_2:+6794998 Msa0585710:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4_2:+6795202 Msa0585710:CDS
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4_2:-6796009 Msa0585720:three_prime_UTR
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4_2:+6795508 Msa0585710:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4_2:+6795067 Msa0585710:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4_2:+6792949 Msa0585710:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4_2:-6796010 Msa0585720:three_prime_UTR
TTTGACGAACTTTGAGGACC+CGG 0.557037 4_2:-6795883 Msa0585720:three_prime_UTR
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4_2:+6794143 Msa0585710:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4_2:-6795849 Msa0585720:three_prime_UTR
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4_2:+6795006 Msa0585710:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4_2:+6795972 Msa0585710:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4_2:-6793255 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4_2:+6794870 Msa0585710:CDS
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4_2:+6795493 Msa0585710:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4_2:+6793467 Msa0585710:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4_2:+6794773 Msa0585710:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4_2:+6792805 Msa0585710:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4_2:+6795798 Msa0585710:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4_2:+6794833 Msa0585710:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4_2:+6792961 Msa0585710:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4_2:+6795794 Msa0585710:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4_2:+6795767 Msa0585710:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4_2:+6795788 Msa0585710:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4_2:+6795831 Msa0585710:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4_2:-6795045 Msa0585720:three_prime_UTR
ATATACCAGGGTATGACAGT+TGG 0.606713 4_2:+6794207 Msa0585710:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4_2:+6793464 Msa0585710:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4_2:+6794801 Msa0585710:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4_2:+6795484 Msa0585710:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4_2:+6794986 Msa0585710:intron
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4_2:+6795801 Msa0585710:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4_2:-6795074 Msa0585720:three_prime_UTR
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4_2:+6795033 Msa0585710:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4_2:-6794809 Msa0585720:three_prime_UTR
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4_2:+6795554 Msa0585710:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4_2:+6795851 Msa0585710:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4_2:+6795418 Msa0585710:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4_2:+6795113 Msa0585710:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4_2:+6795099 Msa0585710:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4_2:-6794813 Msa0585720:three_prime_UTR
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4_2:+6792895 Msa0585710:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4_2:-6795343 Msa0585720:three_prime_UTR
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4_2:+6795852 Msa0585710:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4_2:-6793650 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4_2:-6794212 Msa0585720:three_prime_UTR
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4_2:+6792806 Msa0585710:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4_2:-6794352 Msa0585720:three_prime_UTR
GTCAGAAGGAGAAATTTGTG+AGG 0.668583 4_2:+6792366 Msa0585710:CDS
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4_2:+6794081 Msa0585710:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4_2:+6794796 Msa0585710:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4_2:-6793241 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4_2:+6795114 Msa0585710:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4_2:+6795621 Msa0585710:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4_2:+6795741 Msa0585710:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4_2:+6795555 Msa0585710:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4_2:-6793236 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4_2:+6795832 Msa0585710:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4_2:-6794988 Msa0585720:three_prime_UTR
TCTATATTCAAGCTGCATGG+AGG 0.707083 4_2:+6795770 Msa0585710:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4_2:+6793495 Msa0585710:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4_2:+6794195 Msa0585710:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4_2:+6795115 Msa0585710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTAAAATTCTATAAATTA+TGG - chr4_2:6794620-6794639 Msa0585720:intron 10.0%
!!! AACTATTATTTTGTAGATAA+CGG + chr4_2:6794070-6794089 Msa0585710:intron 15.0%
!! AAATATATATGTATTAGTGT+CGG + chr4_2:6794498-6794517 Msa0585710:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG - chr4_2:6794669-6794688 Msa0585720:intron 15.0%
!!! TTGTGAGGTATTTTTATTTT+TGG + chr4_2:6792381-6792400 Msa0585710:intron 20.0%
!!! TGTGAGGTATTTTTATTTTT+GGG + chr4_2:6792382-6792401 Msa0585710:intron 20.0%
!! TTCAAAAAAAAAAAACCACT+TGG + chr4_2:6792609-6792628 Msa0585710:intron 20.0%
!!! TATTGGTTAATTTAAGAACA+TGG + chr4_2:6792635-6792654 Msa0585710:intron 20.0%
!!! TTATATTCCACTTTAAGTTT+AGG + chr4_2:6792694-6792713 Msa0585710:intron 20.0%
!!! TATATTCCACTTTAAGTTTA+GGG + chr4_2:6792695-6792714 Msa0585710:intron 20.0%
!!! ACTATTATTTTGTAGATAAC+GGG + chr4_2:6794071-6794090 Msa0585710:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG - chr4_2:6794461-6794480 Msa0585720:intron 20.0%
!! GAATCAAAGCATATTTATAA+AGG - chr4_2:6794679-6794698 Msa0585720:intron 20.0%
!! ATGTTTACAGTAAAAACTTT+CGG - chr4_2:6794900-6794919 Msa0585720:intron 20.0%
! GTATAACATATCAATGTGAT+TGG + chr4_2:6792404-6792423 Msa0585710:intron 25.0%
! AGATGCTTATGTTAACAAAT+TGG - chr4_2:6792731-6792750 None:intergenic 25.0%
! AAGATAAGAATCTTCTGTAT+TGG + chr4_2:6793034-6793053 Msa0585710:CDS 25.0%
!!! GATATTTCATTATTGACTTC+TGG + chr4_2:6793328-6793347 Msa0585710:CDS 25.0%
! CAACAAACATTAAGACATAT+TGG - chr4_2:6793452-6793471 None:intergenic 25.0%
! TCTTAAGAAATGAACTTACA+TGG - chr4_2:6793687-6793706 None:intergenic 25.0%
! TATCATTCACATATTCTCTT+TGG + chr4_2:6793802-6793821 Msa0585710:intron 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG - chr4_2:6793846-6793865 None:intergenic 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG + chr4_2:6793846-6793865 Msa0585710:intron 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG - chr4_2:6793878-6793897 None:intergenic 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG - chr4_2:6793879-6793898 None:intergenic 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG - chr4_2:6793919-6793938 None:intergenic 25.0%
! AATTCACATTTCTTGTATTG+TGG + chr4_2:6794170-6794189 Msa0585710:CDS 25.0%
! ATAGACAGTAACAAAATTTG+GGG - chr4_2:6794355-6794374 Msa0585720:three_prime_UTR 25.0%
! CATAGACAGTAACAAAATTT+GGG - chr4_2:6794356-6794375 Msa0585720:three_prime_UTR 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG - chr4_2:6794448-6794467 Msa0585720:intron 25.0%
! TTCCATAGTAATAGCTATTA+TGG + chr4_2:6794831-6794850 Msa0585710:CDS 25.0%
! TCCATAGTAATAGCTATTAT+GGG + chr4_2:6794832-6794851 Msa0585710:CDS 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG - chr4_2:6795170-6795189 Msa0585720:three_prime_UTR 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG + chr4_2:6795201-6795220 Msa0585710:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG + chr4_2:6795202-6795221 Msa0585710:CDS 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG - chr4_2:6795288-6795307 Msa0585720:intron 25.0%
! GTGTATGACATATATTATTC+AGG + chr4_2:6795318-6795337 Msa0585710:intron 25.0%
TACAAGTCACAGTATATTGA+AGG + chr4_2:6792344-6792363 Msa0585710:CDS 30.0%
! GACTCTATTAATCTGGATTT+TGG + chr4_2:6792475-6792494 Msa0585710:intron 30.0%
!! TATTAATCTGGATTTTGGTC+AGG + chr4_2:6792480-6792499 Msa0585710:intron 30.0%
CTCGATAGATTTGTCATTAA+CGG + chr4_2:6792560-6792579 Msa0585710:intron 30.0%
!! AAAAAACCACTTGGTTCTAT+TGG + chr4_2:6792618-6792637 Msa0585710:intron 30.0%
AATTAACCAATAGAACCAAG+TGG - chr4_2:6792627-6792646 None:intergenic 30.0%
TCTTTCAGACTAATATACAG+TGG + chr4_2:6792748-6792767 Msa0585710:intron 30.0%
TAATATACAGTGGATGATGT+AGG + chr4_2:6792758-6792777 Msa0585710:intron 30.0%
!! TCAGATGGACTAATGAATAT+TGG + chr4_2:6792931-6792950 Msa0585710:CDS 30.0%
TACAGAAGATTCTTATCTTG+TGG - chr4_2:6793033-6793052 None:intergenic 30.0%
TACGGAAGATAGAAAAAGAA+AGG - chr4_2:6793105-6793124 None:intergenic 30.0%
TACGATTTGTGGTTGAAATA+CGG - chr4_2:6793123-6793142 None:intergenic 30.0%
! AATATTTTCCGAGAATGGTA+CGG + chr4_2:6793560-6793579 Msa0585710:CDS 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG - chr4_2:6793592-6793611 None:intergenic 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG + chr4_2:6793625-6793644 Msa0585710:CDS 30.0%
ATACCAGATCAAATAGTACA+TGG - chr4_2:6793653-6793672 None:intergenic 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG + chr4_2:6793762-6793781 Msa0585710:intron 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG - chr4_2:6793767-6793786 None:intergenic 30.0%
GGAAGTTTCCATAGAAAAAT+TGG - chr4_2:6793857-6793876 None:intergenic 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG - chr4_2:6793918-6793937 None:intergenic 30.0%
TTGTAGATATTCTGACATAG+AGG + chr4_2:6793974-6793993 Msa0585710:intron 30.0%
GTTAGAATAGATTACGCTAT+AGG + chr4_2:6793996-6794015 Msa0585710:intron 30.0%
AAAGCATGTAAAGAAGTTGA+GGG + chr4_2:6794143-6794162 Msa0585710:CDS 30.0%
!! GATAACTCAGCTTTTAACTA+TGG + chr4_2:6794278-6794297 Msa0585710:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG - chr4_2:6794342-6794361 Msa0585720:three_prime_UTR 30.0%
CCATAGACAGTAACAAAATT+TGG - chr4_2:6794357-6794376 Msa0585720:three_prime_UTR 30.0%
! CCAAATTTTGTTACTGTCTA+TGG + chr4_2:6794354-6794373 Msa0585710:CDS 30.0%
! AATTTTGTTACTGTCTATGG+TGG + chr4_2:6794357-6794376 Msa0585710:CDS 30.0%
! ATTTTGTTACTGTCTATGGT+GGG + chr4_2:6794358-6794377 Msa0585710:CDS 30.0%
ATGGCTAATGTGAAAAACAT+AGG - chr4_2:6794527-6794546 Msa0585720:intron 30.0%
TCTGATCTTCATATCAAAGT+CGG + chr4_2:6794575-6794594 Msa0585710:intron 30.0%
ATTGTAGATTCACTTGATGT+TGG + chr4_2:6794638-6794657 Msa0585710:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG + chr4_2:6794639-6794658 Msa0585710:intron 30.0%
AAGCAAAACAGAAGGATATT+TGG - chr4_2:6794753-6794772 Msa0585720:intron 30.0%
CCATAGTAATAGCTATTATG+GGG + chr4_2:6794833-6794852 Msa0585710:CDS 30.0%
CCAATTGCATTTATAACACA+AGG - chr4_2:6794963-6794982 Msa0585720:intron 30.0%
CCTTGTGTTATAAATGCAAT+TGG + chr4_2:6794960-6794979 Msa0585710:intron 30.0%
GTCTTCTCTGTATATAAACT+CGG - chr4_2:6795403-6795422 Msa0585720:three_prime_UTR 30.0%
AGAGAAGACTTACATAGTTA+GGG + chr4_2:6795414-6795433 Msa0585710:CDS 30.0%
ATATTACGAATCTGATTACC+CGG + chr4_2:6795831-6795850 Msa0585710:CDS 30.0%
ACAGTATATTGAAGGTCAGA+AGG + chr4_2:6792352-6792371 Msa0585710:CDS 35.0%
TTTCCGTTATCTTAACTCTC+TGG + chr4_2:6792437-6792456 Msa0585710:intron 35.0%
GAACCAGAGAGTTAAGATAA+CGG - chr4_2:6792443-6792462 None:intergenic 35.0%
!! TATCTTAACTCTCTGGTTCT+CGG + chr4_2:6792444-6792463 Msa0585710:intron 35.0%
GGAAAGAGACTCTATTAATC+TGG + chr4_2:6792468-6792487 Msa0585710:intron 35.0%
! AATCTATCGAGAGTATATCC+AGG - chr4_2:6792551-6792570 None:intergenic 35.0%
!! TTTTTGAAGAGAGAACCAAG+TGG - chr4_2:6792597-6792616 None:intergenic 35.0%
TGCATACCCTAAACTTAAAG+TGG - chr4_2:6792704-6792723 None:intergenic 35.0%
! ATGATGTAGGTTGGATGATT+TGG + chr4_2:6792771-6792790 Msa0585710:intron 35.0%
ATTGGAAGATCACTCAAAAG+TGG + chr4_2:6792949-6792968 Msa0585710:CDS 35.0%
!! TGTGACACTTTAAGATCTTC+TGG - chr4_2:6792994-6793013 None:intergenic 35.0%
TGGCTAAACAATACGATTTG+TGG - chr4_2:6793134-6793153 None:intergenic 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG - chr4_2:6793154-6793173 None:intergenic 35.0%
CAAAACATATTGAGCATGGA+TGG - chr4_2:6793385-6793404 None:intergenic 35.0%
TACGCAAAACATATTGAGCA+TGG - chr4_2:6793389-6793408 None:intergenic 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG + chr4_2:6793464-6793483 Msa0585710:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG + chr4_2:6793555-6793574 Msa0585710:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG + chr4_2:6793598-6793617 Msa0585710:CDS 35.0%
CATAAGTGATACAGAAGCAA+TGG - chr4_2:6793731-6793750 None:intergenic 35.0%
TCTGTATCACTTATGAAGCT+CGG + chr4_2:6793736-6793755 Msa0585710:intron 35.0%
! GAAGTATCAAGAAGTGTATG+AGG - chr4_2:6793896-6793915 None:intergenic 35.0%
AGAATAGATTACGCTATAGG+CGG + chr4_2:6793999-6794018 Msa0585710:intron 35.0%
GAAACATTGATCATTCGTGT+CGG - chr4_2:6794045-6794064 None:intergenic 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG + chr4_2:6794142-6794161 Msa0585710:CDS 35.0%
AGTTCAAGTAAGCATATACC+AGG + chr4_2:6794194-6794213 Msa0585710:CDS 35.0%
GTTCAAGTAAGCATATACCA+GGG + chr4_2:6794195-6794214 Msa0585710:CDS 35.0%
! TTTTGTTACTGTCTATGGTG+GGG + chr4_2:6794359-6794378 Msa0585710:CDS 35.0%
TGTTATAAAGAGGACTCATG+TGG - chr4_2:6794438-6794457 Msa0585720:intron 35.0%
! TTTTCACATTAGCCATGTAG+AGG + chr4_2:6794531-6794550 Msa0585710:intron 35.0%
ACTGTGAAAAGCAAAACAGA+AGG - chr4_2:6794761-6794780 Msa0585720:intron 35.0%
!! TTTGCTTTTCACAGTACACT+TGG + chr4_2:6794766-6794785 Msa0585710:intron 35.0%
CCCCATAATAGCTATTACTA+TGG - chr4_2:6794836-6794855 Msa0585720:three_prime_UTR 35.0%
TGCAATGAGAAATCTCAGAA+AGG - chr4_2:6795252-6795271 Msa0585720:intron 35.0%
CAGAGAAGACTTACATAGTT+AGG + chr4_2:6795413-6795432 Msa0585710:CDS 35.0%
AAGACTTACATAGTTAGGGA+AGG + chr4_2:6795418-6795437 Msa0585710:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG - chr4_2:6795448-6795467 Msa0585720:three_prime_UTR 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG + chr4_2:6795535-6795554 Msa0585710:CDS 35.0%
TAGACACATACATAGTAGAC+AGG + chr4_2:6795696-6795715 Msa0585710:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG - chr4_2:6795735-6795754 Msa0585720:three_prime_UTR 35.0%
CAATCTATATTCAAGCTGCA+TGG + chr4_2:6795767-6795786 Msa0585710:CDS 35.0%
TATTACGAATCTGATTACCC+GGG + chr4_2:6795832-6795851 Msa0585710:CDS 35.0%
!! CCAAATTTTGACGAACTTTG+AGG - chr4_2:6795892-6795911 Msa0585720:three_prime_UTR 35.0%
CCTCAAAGTTCGTCAAAATT+TGG + chr4_2:6795889-6795908 Msa0585710:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG + chr4_2:6795917-6795936 Msa0585710:CDS 35.0%
GTCAGAAGGAGAAATTTGTG+AGG + chr4_2:6792366-6792385 Msa0585710:CDS 40.0%
!! ATCTTAACTCTCTGGTTCTC+GGG + chr4_2:6792445-6792464 Msa0585710:intron 40.0%
! ATCGAGAGTATATCCAGGTT+CGG - chr4_2:6792546-6792565 None:intergenic 40.0%
ATACAGTGGATGATGTAGGT+TGG + chr4_2:6792762-6792781 Msa0585710:intron 40.0%
! TGTTTGCCTTTTCCTATTCC+CGG + chr4_2:6792856-6792875 Msa0585710:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG - chr4_2:6792882-6792901 None:intergenic 40.0%
! TCGTCGAAATCATTTCGAGT+CGG + chr4_2:6792895-6792914 Msa0585710:CDS 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG + chr4_2:6792906-6792925 Msa0585710:CDS 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG + chr4_2:6792907-6792926 Msa0585710:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG + chr4_2:6792960-6792979 Msa0585710:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG - chr4_2:6793258-6793277 None:intergenic 40.0%
TCATCTCGAGTATTTGGAAG+AGG + chr4_2:6793258-6793277 Msa0585710:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG + chr4_2:6793467-6793486 Msa0585710:intron 40.0%
AATGGTACGGTTCATACCTT+TGG + chr4_2:6793573-6793592 Msa0585710:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG + chr4_2:6793647-6793666 Msa0585710:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG + chr4_2:6793901-6793920 Msa0585710:intron 40.0%
ATATACCAGGGTATGACAGT+TGG + chr4_2:6794207-6794226 Msa0585710:CDS 40.0%
GAAGATCAGACACACGATAT+GGG - chr4_2:6794565-6794584 Msa0585720:intron 40.0%
TGAAGATCAGACACACGATA+TGG - chr4_2:6794566-6794585 Msa0585720:intron 40.0%
! TTTTCACAGTACACTTGGTC+AGG + chr4_2:6794771-6794790 Msa0585710:intron 40.0%
TTTCACAGTACACTTGGTCA+GGG + chr4_2:6794772-6794791 Msa0585710:intron 40.0%
TGGAAAACAAAACCTCTCCA+GGG - chr4_2:6794816-6794835 Msa0585720:three_prime_UTR 40.0%
ATGGAAAACAAAACCTCTCC+AGG - chr4_2:6794817-6794836 Msa0585720:three_prime_UTR 40.0%
TCTTCTGATTGGAAACATGC+AGG + chr4_2:6794870-6794889 Msa0585710:CDS 40.0%
GACCTACCTTCAATCAATGT+CGG + chr4_2:6794986-6795005 Msa0585710:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG - chr4_2:6794991-6795010 Msa0585720:three_prime_UTR 40.0%
TGGAGGAAATGAGGATCAAA+AGG + chr4_2:6795015-6795034 Msa0585710:CDS 40.0%
! AATTTTCTTCATCGACCCCT+CGG - chr4_2:6795133-6795152 Msa0585720:three_prime_UTR 40.0%
AGGGGTCGATGAAGAAAATT+TGG + chr4_2:6795133-6795152 Msa0585710:CDS 40.0%
GAGATGTTGTTCATCATTCG+TGG + chr4_2:6795454-6795473 Msa0585710:CDS 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG + chr4_2:6795481-6795500 Msa0585710:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG + chr4_2:6795519-6795538 Msa0585710:CDS 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG + chr4_2:6795523-6795542 Msa0585710:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG + chr4_2:6795555-6795574 Msa0585710:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG - chr4_2:6795610-6795629 Msa0585720:three_prime_UTR 40.0%
TACCTCACTCATAGCTTTCA+CGG - chr4_2:6795626-6795645 Msa0585720:three_prime_UTR 40.0%
TCCGTTTCTATTCGCAACAA+TGG + chr4_2:6795731-6795750 Msa0585710:CDS 40.0%
ATTCGCAACAATGGAGAACA+TGG + chr4_2:6795740-6795759 Msa0585710:CDS 40.0%
TTCGCAACAATGGAGAACAT+GGG + chr4_2:6795741-6795760 Msa0585710:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG + chr4_2:6795770-6795789 Msa0585710:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG - chr4_2:6796021-6796040 Msa0585720:three_prime_UTR 40.0%
!! TCTTAACTCTCTGGTTCTCG+GGG + chr4_2:6792446-6792465 Msa0585710:intron 45.0%
ACGGAAGTTCATCAACCACT+TGG + chr4_2:6792579-6792598 Msa0585710:intron 45.0%
!! GCTAGATGGCATTGACGAAT+TGG - chr4_2:6792800-6792819 None:intergenic 45.0%
! TCAATGCCATCTAGCTCAAG+TGG + chr4_2:6792805-6792824 Msa0585710:CDS 45.0%
! CAATGCCATCTAGCTCAAGT+GGG + chr4_2:6792806-6792825 Msa0585710:CDS 45.0%
TTCATCCCACTTGAGCTAGA+TGG - chr4_2:6792814-6792833 None:intergenic 45.0%
GGAGTGAAAAAGGGATCAGA+TGG + chr4_2:6792916-6792935 Msa0585710:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG + chr4_2:6792959-6792978 Msa0585710:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG + chr4_2:6792961-6792980 Msa0585710:CDS 45.0%
! AGATGAAGGTGCAATGAAGG+CGG - chr4_2:6793244-6793263 None:intergenic 45.0%
! TCGAGATGAAGGTGCAATGA+AGG - chr4_2:6793247-6793266 None:intergenic 45.0%
GCACCTTCATCTCGAGTATT+TGG + chr4_2:6793252-6793271 Msa0585710:CDS 45.0%
GGTGAACTTGTCATAGATCC+TGG + chr4_2:6793279-6793298 Msa0585710:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG - chr4_2:6793300-6793319 None:intergenic 45.0%
TGGATGGTATCTCAAACCTG+TGG - chr4_2:6793369-6793388 None:intergenic 45.0%
TCTTTATAGCGCAAGACGCA+TGG + chr4_2:6793492-6793511 Msa0585710:CDS 45.0%
GGTATGAACCGTACCATTCT+CGG - chr4_2:6793571-6793590 None:intergenic 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG + chr4_2:6794081-6794100 Msa0585710:intron 45.0%
TAGAACGTAACGACACATGC+TGG + chr4_2:6794117-6794136 Msa0585710:CDS 45.0%
TTTCTCCAACTGTCATACCC+TGG - chr4_2:6794215-6794234 Msa0585720:three_prime_UTR 45.0%
GGTGGGGACTTCAGAATTTA+AGG + chr4_2:6794375-6794394 Msa0585710:CDS 45.0%
TTCACAGTACACTTGGTCAG+GGG + chr4_2:6794773-6794792 Msa0585710:intron 45.0%
AAACAAAACCTCTCCAGGGT+AGG - chr4_2:6794812-6794831 Msa0585720:three_prime_UTR 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG + chr4_2:6794859-6794878 Msa0585710:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG - chr4_2:6794995-6795014 Msa0585720:three_prime_UTR 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG + chr4_2:6794995-6795014 Msa0585710:CDS 45.0%
GGGTGTTCAACCACTTGTAT+TGG - chr4_2:6795112-6795131 Msa0585720:three_prime_UTR 45.0%
! ATTTTCTTCATCGACCCCTC+GGG - chr4_2:6795132-6795151 Msa0585720:three_prime_UTR 45.0%
GGTTCCTCTCTTTGCTAACA+TGG + chr4_2:6795339-6795358 Msa0585710:intron 45.0%
TCGTCCATGTTAGCAAAGAG+AGG - chr4_2:6795346-6795365 Msa0585720:three_prime_UTR 45.0%
!! GAGAGTGTTACTACAGATGG+TGG + chr4_2:6795484-6795503 Msa0585710:CDS 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG + chr4_2:6795493-6795512 Msa0585710:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG + chr4_2:6795508-6795527 Msa0585710:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG + chr4_2:6795554-6795573 Msa0585710:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG + chr4_2:6795561-6795580 Msa0585710:CDS 45.0%
AACCGTGAAAGCTATGAGTG+AGG + chr4_2:6795621-6795640 Msa0585710:CDS 45.0%
GAAAGCTATGAGTGAGGTAG+AGG + chr4_2:6795627-6795646 Msa0585710:CDS 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG + chr4_2:6795648-6795667 Msa0585710:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG + chr4_2:6795782-6795801 Msa0585710:CDS 45.0%
TTGCAGAATCATCACTTCCC+GGG - chr4_2:6795852-6795871 Msa0585720:three_prime_UTR 45.0%
CTTGCAGAATCATCACTTCC+CGG - chr4_2:6795853-6795872 Msa0585720:three_prime_UTR 45.0%
GGGAAGTGATGATTCTGCAA+GGG + chr4_2:6795852-6795871 Msa0585710:CDS 45.0%
! TTTGACGAACTTTGAGGACC+CGG - chr4_2:6795886-6795905 Msa0585720:three_prime_UTR 45.0%
AGTACCAAGTTCAAGAGAGC+TGG + chr4_2:6795972-6795991 Msa0585710:CDS 45.0%
TTCACCAGCTCTCTTGAACT+TGG - chr4_2:6795979-6795998 Msa0585720:three_prime_UTR 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG - chr4_2:6796005-6796024 Msa0585720:three_prime_UTR 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG - chr4_2:6796006-6796025 Msa0585720:three_prime_UTR 45.0%
! GCACTGAAATCAGGTTCTGA+GGG - chr4_2:6796012-6796031 Msa0585720:three_prime_UTR 45.0%
! AGCACTGAAATCAGGTTCTG+AGG - chr4_2:6796013-6796032 Msa0585720:three_prime_UTR 45.0%
!!! AATTGAATATAAATAAATTT+TGG - chr4_2:6793930-6793949 None:intergenic 5.0%
!! CTTAACTCTCTGGTTCTCGG+GGG + chr4_2:6792447-6792466 Msa0585710:intron 50.0%
TTCCAGTCAAGATCCGAACC+TGG + chr4_2:6792530-6792549 Msa0585710:intron 50.0%
ATCCAGGTTCGGATCTTGAC+TGG - chr4_2:6792535-6792554 None:intergenic 50.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG - chr4_2:6792877-6792896 None:intergenic 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG - chr4_2:6792878-6792897 None:intergenic 50.0%
GTATCTCAAACCTGTGGCAG+TGG - chr4_2:6793363-6793382 None:intergenic 50.0%
TTATAGCGCAAGACGCATGG+AGG + chr4_2:6793495-6793514 Msa0585710:CDS 50.0%
CTTCTGACTAGTACCTACCC+TGG + chr4_2:6794796-6794815 Msa0585710:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG + chr4_2:6794998-6795017 Msa0585710:CDS 50.0%
CTGACTCTTTCCCTTAGGTC+CGG - chr4_2:6795080-6795099 Msa0585720:three_prime_UTR 50.0%
AGCGTCTGACTCTTTCCCTT+AGG - chr4_2:6795085-6795104 Msa0585720:three_prime_UTR 50.0%
GACGCTACGACCAATACAAG+TGG + chr4_2:6795099-6795118 Msa0585710:CDS 50.0%
TACAAGTGGTTGAACACCCG+AGG + chr4_2:6795113-6795132 Msa0585710:CDS 50.0%
ACAAGTGGTTGAACACCCGA+GGG + chr4_2:6795114-6795133 Msa0585710:CDS 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG - chr4_2:6795589-6795608 Msa0585720:three_prime_UTR 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG + chr4_2:6795788-6795807 Msa0585710:CDS 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG + chr4_2:6795794-6795813 Msa0585710:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG + chr4_2:6795798-6795817 Msa0585710:CDS 50.0%
CGGGAAGTGATGATTCTGCA+AGG + chr4_2:6795851-6795870 Msa0585710:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG + chr4_2:6795858-6795877 Msa0585710:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG + chr4_2:6795937-6795956 Msa0585710:CDS 50.0%
CAATGAAGGCGGTGCGGAAT+TGG - chr4_2:6793233-6793252 None:intergenic 55.0%
AAGGTGCAATGAAGGCGGTG+CGG - chr4_2:6793239-6793258 None:intergenic 55.0%
! CGCAAGACGCATGGAGGTAT+TGG + chr4_2:6793501-6793520 Msa0585710:CDS 55.0%
TGGGCACATGCACCTCTACA+TGG - chr4_2:6794546-6794565 Msa0585720:intron 55.0%
GACTAGTACCTACCCTGGAG+AGG + chr4_2:6794801-6794820 Msa0585710:CDS 55.0%
CGGTTCGTCTGGAGGAAATG+AGG + chr4_2:6795006-6795025 Msa0585710:CDS 55.0%
GCGATGATGCATCCACTGCT+CGG - chr4_2:6795048-6795067 Msa0585720:three_prime_UTR 55.0%
GCATCATCGCTTGCTTCCTC+CGG + chr4_2:6795058-6795077 Msa0585710:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG + chr4_2:6795067-6795086 Msa0585710:CDS 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG - chr4_2:6795077-6795096 Msa0585720:three_prime_UTR 55.0%
CAAGTGGTTGAACACCCGAG+GGG + chr4_2:6795115-6795134 Msa0585710:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG + chr4_2:6795864-6795883 Msa0585710:CDS 55.0%
! CCTTTTCCTATTCCCGGCCG+CGG + chr4_2:6792862-6792881 Msa0585710:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG + chr4_2:6792863-6792882 Msa0585710:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG - chr4_2:6792871-6792890 None:intergenic 60.0%
GTCTGTACTGCCACTGCCAC+AGG + chr4_2:6793350-6793369 Msa0585710:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG + chr4_2:6795033-6795052 Msa0585710:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG + chr4_2:6795066-6795085 Msa0585710:CDS 60.0%
AAGGCGAAGGCGAAGGAAGG+AGG + chr4_2:6795801-6795820 Msa0585710:CDS 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG + chr4_2:6795865-6795884 Msa0585710:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG - chr4_2:6792865-6792884 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 6792329 6796050 6792329 ID=Msa0585710;Name=Msa0585710
chr4_2 mRNA 6792329 6796050 6792329 ID=Msa0585710-mRNA-1;Parent=Msa0585710;Name=Msa0585710-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|0.85|1|1|7|0|744
chr4_2 exon 6792329 6792387 6792329 ID=Msa0585710-mRNA-1:exon:1677;Parent=Msa0585710-mRNA-1
chr4_2 exon 6792780 6793371 6792780 ID=Msa0585710-mRNA-1:exon:1678;Parent=Msa0585710-mRNA-1
chr4_2 exon 6793475 6793687 6793475 ID=Msa0585710-mRNA-1:exon:1679;Parent=Msa0585710-mRNA-1
chr4_2 exon 6794086 6794396 6794086 ID=Msa0585710-mRNA-1:exon:1680;Parent=Msa0585710-mRNA-1
chr4_2 exon 6794780 6794891 6794780 ID=Msa0585710-mRNA-1:exon:1681;Parent=Msa0585710-mRNA-1
chr4_2 exon 6794987 6795223 6794987 ID=Msa0585710-mRNA-1:exon:1682;Parent=Msa0585710-mRNA-1
chr4_2 exon 6795340 6796050 6795340 ID=Msa0585710-mRNA-1:exon:1683;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6792329 6792387 6792329 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6792780 6793371 6792780 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6793475 6793687 6793475 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6794086 6794396 6794086 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6794780 6794891 6794780 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6794987 6795223 6794987 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
chr4_2 CDS 6795340 6796050 6795340 ID=Msa0585710-mRNA-1:cds;Parent=Msa0585710-mRNA-1
Gene Sequence

>Msa0585710

ATGTTTGACTCTGGTTACAAGTCACAGTATATTGAAGGTCAGAAGGAGAAATTTGTGAGGTTGGATGATTTGGATTCCAATTCGTCAATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCACAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>Msa0585710

MFDSGYKSQYIEGQKEKFVRLDDLDSNSSMPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE*