AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0663700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663700 MtrunA17_Chr4g0001481 97.063 715 20 1 1 715 30 743 0.0 1438
Msa0663700 MtrunA17_Chr4g0073941 67.052 692 204 8 26 710 53 727 0.0 937
Msa0663700 MtrunA17_Chr1g0158861 53.729 657 276 8 68 715 79 716 0.0 713
Msa0663700 MtrunA17_Chr3g0126541 54.671 653 267 7 68 710 77 710 0.0 711
Msa0663700 MtrunA17_Chr2g0305771 52.199 682 293 6 53 715 24 691 0.0 708
Msa0663700 MtrunA17_Chr3g0139071 52.246 668 295 7 60 710 61 721 0.0 676
Msa0663700 MtrunA17_Chr2g0326871 50.000 712 318 14 4 709 22 701 0.0 672
Msa0663700 MtrunA17_Chr7g0275581 51.740 661 287 7 63 710 74 715 0.0 664
Msa0663700 MtrunA17_Chr4g0028861 55.150 602 257 7 40 637 90 682 0.0 663
Msa0663700 MtrunA17_Chr1g0181121 51.420 669 293 13 60 713 32 683 0.0 655
Msa0663700 MtrunA17_Chr7g0228181 51.156 649 294 10 71 709 43 678 0.0 655
Msa0663700 MtrunA17_Chr3g0125651 47.661 684 317 12 61 714 20 692 0.0 629
Msa0663700 MtrunA17_Chr8g0347121 47.230 722 327 11 28 708 15 723 0.0 628
Msa0663700 MtrunA17_Chr6g0481021 40.956 586 310 15 72 637 123 692 4.46e-128 396
Msa0663700 MtrunA17_Chr6g0480851 38.889 594 327 14 72 649 184 757 4.82e-121 379
Msa0663700 MtrunA17_Chr6g0480671 39.626 588 324 12 71 643 192 763 6.80e-121 379
Msa0663700 MtrunA17_Chr6g0480781 36.275 612 343 14 72 657 169 759 1.65e-117 370
Msa0663700 MtrunA17_Chr8g0360821 35.777 573 348 10 74 635 64 627 2.91e-110 348
Msa0663700 MtrunA17_Chr7g0218341 36.333 589 354 9 57 637 102 677 3.80e-109 347
Msa0663700 MtrunA17_Chr6g0480741 35.495 586 335 15 71 635 114 677 9.94e-98 316
Msa0663700 MtrunA17_Chr6g0480681 33.594 640 350 18 72 660 115 730 1.19e-91 301
Msa0663700 MtrunA17_Chr2g0318561 36.245 458 273 9 188 635 2 450 3.31e-91 293
Msa0663700 MtrunA17_Chr5g0395421 38.162 359 186 11 270 613 18 355 1.71e-60 208
Msa0663700 MtrunA17_Chr6g0480771 39.113 248 128 6 396 630 12 249 2.91e-42 155
Msa0663700 MtrunA17_Chr2g0326861 40.000 205 114 4 147 349 14 211 6.64e-40 146
Msa0663700 MtrunA17_Chr6g0480841 41.237 194 94 5 450 630 2 188 1.72e-34 130
Msa0663700 MtrunA17_Chr5g0395411 35.821 201 114 4 72 271 172 358 1.94e-32 129
Msa0663700 MtrunA17_Chr2g0326851 43.262 141 62 5 575 709 3 131 2.52e-27 107
Msa0663700 MtrunA17_Chr6g0480751 36.567 134 85 0 71 204 97 230 2.58e-23 99.4
Msa0663700 MtrunA17_Chr6g0480791 40.164 122 72 1 72 192 172 293 7.06e-22 96.7
Msa0663700 MtrunA17_Chr4g0063141 25.366 205 150 2 350 553 239 441 3.49e-17 86.3
Msa0663700 MtrunA17_Chr3g0138671 25.616 203 148 2 351 553 243 442 2.51e-16 83.2
Msa0663700 MtrunA17_Chr2g0277651 24.762 210 153 3 346 553 256 462 5.56e-15 79.0
Msa0663700 MtrunA17_Chr5g0434371 24.194 186 138 2 345 530 277 459 1.02e-12 71.6
Msa0663700 MtrunA17_Chr6g0480761 28.859 149 97 5 214 360 24 165 2.18e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0663700 AT1G19780.1 68.306 732 209 10 1 715 26 751 0.0 987
Msa0663700 AT1G15990.1 67.906 726 209 12 1 710 20 737 0.0 966
Msa0663700 AT2G23980.6 63.333 720 242 8 3 710 8 717 0.0 927
Msa0663700 AT5G57940.3 66.040 692 217 5 23 710 32 709 0.0 927
Msa0663700 AT2G23980.9 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663700 AT2G23980.10 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663700 AT2G23980.1 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663700 AT2G23980.4 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663700 AT2G23980.5 63.333 720 242 8 3 710 35 744 0.0 926
Msa0663700 AT5G57940.1 66.040 692 217 5 23 710 39 716 0.0 926
Msa0663700 AT5G57940.2 66.040 692 217 5 23 710 39 716 0.0 926
Msa0663700 AT4G30560.1 63.928 718 227 7 3 710 34 729 0.0 925
Msa0663700 AT4G30560.4 63.928 718 227 7 3 710 34 729 0.0 925
Msa0663700 AT4G30560.5 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663700 AT4G30560.2 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663700 AT4G30560.3 63.928 718 227 7 3 710 8 703 0.0 924
Msa0663700 AT2G23980.3 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663700 AT2G23980.2 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663700 AT2G23980.8 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663700 AT2G23980.7 61.373 743 242 9 3 710 35 767 0.0 913
Msa0663700 AT1G19780.2 70.425 541 145 7 188 715 2 540 0.0 753
Msa0663700 AT1G01340.2 53.707 661 279 6 63 710 59 705 0.0 717
Msa0663700 AT1G01340.1 53.707 661 279 6 63 710 54 700 0.0 717
Msa0663700 AT4G01010.1 51.489 705 293 11 24 712 24 695 0.0 704
Msa0663700 AT4G01010.2 51.489 705 293 11 24 712 24 695 0.0 704
Msa0663700 AT5G53130.1 52.388 670 285 7 51 710 69 714 0.0 690
Msa0663700 AT2G24610.1 52.444 675 287 11 60 710 60 724 0.0 681
Msa0663700 AT2G28260.1 50.946 687 305 8 26 710 21 677 0.0 681
Msa0663700 AT2G28260.2 52.024 667 289 7 45 710 1 637 0.0 679
Msa0663700 AT2G24610.2 52.444 675 287 11 60 710 75 739 0.0 679
Msa0663700 AT4G30360.1 51.672 658 299 10 70 714 69 720 0.0 655
Msa0663700 AT2G46430.1 50.383 653 287 8 71 708 71 701 0.0 649
Msa0663700 AT2G46430.2 50.383 653 287 8 71 708 71 701 0.0 649
Msa0663700 AT5G14870.1 49.552 670 303 9 69 709 37 700 0.0 647
Msa0663700 AT3G48010.1 47.640 678 303 12 69 708 41 704 0.0 622
Msa0663700 AT2G46440.1 43.339 623 309 13 70 677 28 621 8.88e-163 483
Msa0663700 AT2G46450.4 38.473 655 342 16 70 706 28 639 5.75e-149 449
Msa0663700 AT2G46450.1 38.473 655 342 16 70 706 28 639 5.75e-149 449
Msa0663700 AT2G46450.2 37.557 655 325 17 70 706 28 616 9.70e-141 427
Msa0663700 AT2G46450.3 37.557 655 325 17 70 706 15 603 1.17e-140 426
Msa0663700 AT2G46440.2 47.097 465 208 10 227 677 3 443 1.49e-130 394
Msa0663700 AT3G17690.1 37.563 591 338 14 61 638 147 719 1.77e-119 375
Msa0663700 AT3G17700.1 37.544 570 328 10 72 629 192 745 1.76e-115 365
Msa0663700 AT5G15410.2 36.678 578 347 9 65 629 105 676 1.67e-108 346
Msa0663700 AT5G15410.1 36.678 578 347 9 65 629 106 677 2.23e-108 345
Msa0663700 AT5G54250.2 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663700 AT5G54250.1 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663700 AT5G54250.4 34.549 576 361 8 71 635 78 648 1.12e-107 343
Msa0663700 AT3G17690.2 37.897 504 291 10 61 560 147 632 6.54e-106 337
Msa0663700 AT3G17700.2 37.652 494 291 7 72 564 192 669 2.66e-103 331
Msa0663700 AT5G54250.3 33.854 576 360 9 71 635 78 643 3.90e-102 328
Msa0663700 AT3G17690.3 35.433 381 231 8 61 437 147 516 1.30e-66 229
Msa0663700 AT4G22200.2 24.020 204 152 2 350 553 263 463 1.97e-16 83.6
Msa0663700 AT4G22200.1 24.020 204 152 2 350 553 263 463 5.67e-16 82.8
Msa0663700 AT5G37500.1 21.489 470 274 13 70 532 55 436 1.11e-14 78.6
Msa0663700 AT5G37500.4 20.726 468 280 12 70 532 55 436 1.47e-14 78.2
Msa0663700 AT5G37500.2 21.702 470 273 13 70 532 55 436 1.57e-14 78.2
Msa0663700 AT2G26650.1 23.902 205 153 2 350 553 239 441 2.89e-14 77.4
Msa0663700 AT5G37500.3 20.833 408 252 9 126 532 45 382 3.13e-14 77.0
Msa0663700 AT2G26650.2 23.902 205 153 2 350 553 134 336 4.39e-14 76.6
Msa0663700 AT5G46240.1 22.727 198 152 1 350 547 246 442 6.08e-14 75.9
Msa0663700 AT3G02850.2 20.902 488 293 14 54 534 56 457 1.11e-13 75.1
Msa0663700 AT3G02850.1 20.902 488 293 14 54 534 56 457 1.18e-13 75.1
Msa0663700 AT3G02850.3 24.479 192 140 3 345 534 269 457 2.04e-13 73.9
Msa0663700 AT4G32650.2 23.153 203 153 2 351 553 276 475 1.53e-12 71.2
Msa0663700 AT4G32650.3 23.153 203 153 2 351 553 276 475 1.99e-12 71.2
Msa0663700 AT4G32650.1 23.153 203 153 2 351 553 276 475 2.08e-12 70.9

Find 142 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4_4:-2595953 Msa0663700:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4_4:+2595198 Msa0663690:three_prime_UTR
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4_4:-2596409 Msa0663700:intron
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4_4:-2594895 Msa0663700:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4_4:-2597526 Msa0663700:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4_4:+2596430 Msa0663690:three_prime_UTR
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4_4:-2594920 Msa0663700:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4_4:-2595002 Msa0663700:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4_4:+2597787 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4_4:+2595617 Msa0663690:three_prime_UTR
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4_4:-2597872 Msa0663700:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4_4:+2597627 None:intergenic
CAGAGAAGACTTACATAGTT+AGG 0.283571 4_4:-2595371 Msa0663700:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4_4:-2595651 Msa0663700:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4_4:+2595951 Msa0663690:three_prime_UTR
GATATTTCATTATTGACTTC+TGG 0.297261 4_4:-2597450 Msa0663700:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4_4:-2594919 Msa0663700:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4_4:+2596445 Msa0663690:three_prime_UTR
CATAGACAGTAACAAAATTT+GGG 0.315147 4_4:+2596431 Msa0663690:three_prime_UTR
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4_4:-2595925 Msa0663700:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4_4:+2597548 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4_4:-2595952 Msa0663700:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4_4:+2594782 Msa0663690:three_prime_UTR
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4_4:-2597282 Msa0663700:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4_4:+2594781 Msa0663690:three_prime_UTR
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4_4:+2597967 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4_4:-2597847 Msa0663700:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4_4:-2595789 Msa0663700:CDS
AATGGTACGGTTCATACCTT+TGG 0.384471 4_4:-2597210 Msa0663700:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4_4:-2594867 Msa0663700:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4_4:+2594766 Msa0663690:three_prime_UTR
ATGAACAACATCTCATCAAC+TGG 0.395916 4_4:+2595339 Msa0663690:three_prime_UTR
AAGATAAGAATCTTCTGTAT+TGG 0.412931 4_4:-2597744 Msa0663700:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4_4:+2594934 Msa0663690:three_prime_UTR
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4_4:-2594847 Msa0663700:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4_4:+2595177 Msa0663690:three_prime_UTR
TACCTCACTCATAGCTTTCA+CGG 0.425446 4_4:+2595161 Msa0663690:three_prime_UTR
TACGATTTGTGGTTGAAATA+CGG 0.428941 4_4:+2597658 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4_4:-2597428 Msa0663700:intron
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4_4:-2597819 Msa0663700:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4_4:+2595052 Msa0663690:three_prime_UTR
GTGCCATGTACTATTTGATC+TGG 0.449942 4_4:-2597136 Msa0663700:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4_4:+2595675 Msa0663690:three_prime_UTR
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4_4:+2595970 Msa0663690:three_prime_UTR
TGGCTAAACAATACGATTTG+TGG 0.457799 4_4:+2597647 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4_4:+2597215 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4_4:-2595726 Msa0663700:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4_4:-2595718 Msa0663700:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4_4:-2597499 Msa0663700:CDS
TACAGAAGATTCTTATCTTG+TGG 0.474072 4_4:+2597748 None:intergenic
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4_4:+2597916 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4_4:+2594808 Msa0663690:three_prime_UTR
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4_4:+2595707 Msa0663690:three_prime_UTR
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4_4:+2595702 Msa0663690:three_prime_UTR
AGAGAAGACTTACATAGTTA+GGG 0.493349 4_4:-2595370 Msa0663700:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4_4:+2597481 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4_4:-2596667 Msa0663700:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4_4:-2596642 Msa0663700:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4_4:-2595445 Msa0663700:CDS
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4_4:-2595136 Msa0663700:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4_4:-2596614 Msa0663700:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4_4:-2595583 Msa0663700:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4_4:-2597818 Msa0663700:CDS
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4_4:+2597534 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4_4:-2595044 Msa0663700:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4_4:+2595792 Msa0663690:three_prime_UTR
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4_4:+2597418 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4_4:-2595769 Msa0663700:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4_4:-2595053 Msa0663700:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4_4:-2597291 Msa0663700:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4_4:-2595223 Msa0663700:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4_4:-2594926 Msa0663700:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4_4:-2597520 Msa0663700:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4_4:-2596012 Msa0663700:intron
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4_4:-2595157 Msa0663700:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4_4:-2595330 Msa0663700:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4_4:-2595303 Msa0663700:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4_4:-2596590 Msa0663700:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4_4:+2595384 Msa0663690:three_prime_UTR
TAGACACATACATAGTAGAC+AGG 0.544177 4_4:-2595088 Msa0663700:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4_4:-2595786 Msa0663700:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4_4:-2595582 Msa0663700:intron
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4_4:+2594775 Msa0663690:three_prime_UTR
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4_4:-2595276 Msa0663700:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4_4:-2595717 Msa0663700:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4_4:-2597829 Msa0663700:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4_4:+2594774 Msa0663690:three_prime_UTR
TTTGACGAACTTTGAGGACC+CGG 0.557037 4_4:+2594901 Msa0663690:three_prime_UTR
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4_4:-2596641 Msa0663700:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4_4:+2594935 Msa0663690:three_prime_UTR
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4_4:-2595778 Msa0663700:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4_4:-2594812 Msa0663700:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4_4:+2597523 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4_4:-2595914 Msa0663700:intron
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4_4:-2595291 Msa0663700:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4_4:-2597316 Msa0663700:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4_4:-2596011 Msa0663700:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4_4:-2597973 Msa0663700:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4_4:-2594986 Msa0663700:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4_4:-2595951 Msa0663700:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4_4:-2597817 Msa0663700:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4_4:-2594990 Msa0663700:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4_4:-2595017 Msa0663700:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4_4:-2594996 Msa0663700:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4_4:-2594953 Msa0663700:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4_4:+2595739 Msa0663690:three_prime_UTR
ATATACCAGGGTATGACAGT+TGG 0.606713 4_4:-2596577 Msa0663700:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4_4:-2597319 Msa0663700:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4_4:-2595983 Msa0663700:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4_4:-2595300 Msa0663700:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4_4:-2595798 Msa0663700:CDS
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4_4:-2594983 Msa0663700:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4_4:+2595710 Msa0663690:three_prime_UTR
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4_4:-2595751 Msa0663700:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4_4:+2595975 Msa0663690:three_prime_UTR
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4_4:-2595230 Msa0663700:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4_4:-2594933 Msa0663700:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4_4:-2595366 Msa0663700:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4_4:-2595671 Msa0663700:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4_4:-2595685 Msa0663700:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4_4:+2595971 Msa0663690:three_prime_UTR
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4_4:-2597883 Msa0663700:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4_4:+2595441 Msa0663690:three_prime_UTR
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4_4:-2594932 Msa0663700:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4_4:+2597133 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4_4:+2596572 Msa0663690:three_prime_UTR
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4_4:-2597972 Msa0663700:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4_4:+2596432 Msa0663690:three_prime_UTR
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4_4:-2596703 Msa0663700:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4_4:-2595988 Msa0663700:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4_4:+2597537 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4_4:-2595670 Msa0663700:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4_4:-2595163 Msa0663700:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4_4:-2595043 Msa0663700:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4_4:-2595229 Msa0663700:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4_4:+2597542 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4_4:-2594952 Msa0663700:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4_4:+2595796 Msa0663690:three_prime_UTR
TCTATATTCAAGCTGCATGG+AGG 0.707083 4_4:-2595014 Msa0663700:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4_4:-2597288 Msa0663700:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4_4:-2596589 Msa0663700:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4_4:-2595669 Msa0663700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTAAAATTCTATAAATTA+TGG + chr4_4:2596562-2596581 Msa0663690:three_prime_UTR 10.0%
!!! AACTATTATTTTGTAGATAA+CGG - chr4_4:2596012-2596031 Msa0663700:intron 15.0%
!! AAATATATATGTATTAGTGT+CGG - chr4_4:2596440-2596459 Msa0663700:CDS 15.0%
!! ATATTTATAAAGGATGATAT+AGG + chr4_4:2596611-2596630 Msa0663690:three_prime_UTR 15.0%
!!! ACTATTATTTTGTAGATAAC+GGG - chr4_4:2596013-2596032 Msa0663700:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG + chr4_4:2596403-2596422 Msa0663690:intron 20.0%
!! GAATCAAAGCATATTTATAA+AGG + chr4_4:2596621-2596640 Msa0663690:three_prime_UTR 20.0%
!! ATGTTTACAGTAAAAACTTT+CGG + chr4_4:2596842-2596861 None:intergenic 20.0%
! AAGATAAGAATCTTCTGTAT+TGG - chr4_4:2594982-2595001 Msa0663700:CDS 25.0%
!!! GATATTTCATTATTGACTTC+TGG - chr4_4:2595276-2595295 Msa0663700:CDS 25.0%
! CAACAAACATTAAGACATAT+TGG + chr4_4:2595395-2595414 Msa0663690:three_prime_UTR 25.0%
! TCTTAAGAAATGAACTTACA+TGG + chr4_4:2595630-2595649 Msa0663690:three_prime_UTR 25.0%
! TATCATTCACATATTCTCTT+TGG - chr4_4:2595745-2595764 Msa0663700:CDS 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG + chr4_4:2595789-2595808 Msa0663690:three_prime_UTR 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG - chr4_4:2595789-2595808 Msa0663700:CDS 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG + chr4_4:2595821-2595840 Msa0663690:intron 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG + chr4_4:2595822-2595841 Msa0663690:intron 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG + chr4_4:2595862-2595881 Msa0663690:intron 25.0%
! AATTCACATTTCTTGTATTG+TGG - chr4_4:2596112-2596131 Msa0663700:intron 25.0%
! ATAGACAGTAACAAAATTTG+GGG + chr4_4:2596297-2596316 Msa0663690:intron 25.0%
! CATAGACAGTAACAAAATTT+GGG + chr4_4:2596298-2596317 Msa0663690:intron 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG + chr4_4:2596390-2596409 Msa0663690:intron 25.0%
! TTCCATAGTAATAGCTATTA+TGG - chr4_4:2596773-2596792 Msa0663700:intron 25.0%
! TCCATAGTAATAGCTATTAT+GGG - chr4_4:2596774-2596793 Msa0663700:intron 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG + chr4_4:2597112-2597131 None:intergenic 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG - chr4_4:2597143-2597162 Msa0663700:CDS 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG - chr4_4:2597144-2597163 Msa0663700:CDS 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG + chr4_4:2597230-2597249 None:intergenic 25.0%
! GTGTATGACATATATTATTC+AGG - chr4_4:2597260-2597279 Msa0663700:CDS 25.0%
!! TCAGATGGACTAATGAATAT+TGG - chr4_4:2594879-2594898 Msa0663700:CDS 30.0%
TACAGAAGATTCTTATCTTG+TGG + chr4_4:2594981-2595000 Msa0663690:three_prime_UTR 30.0%
TACGGAAGATAGAAAAAGAA+AGG + chr4_4:2595053-2595072 Msa0663690:three_prime_UTR 30.0%
TACGATTTGTGGTTGAAATA+CGG + chr4_4:2595071-2595090 Msa0663690:three_prime_UTR 30.0%
! AATATTTTCCGAGAATGGTA+CGG - chr4_4:2595503-2595522 Msa0663700:intron 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG + chr4_4:2595535-2595554 Msa0663690:intron 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG - chr4_4:2595568-2595587 Msa0663700:intron 30.0%
ATACCAGATCAAATAGTACA+TGG + chr4_4:2595596-2595615 Msa0663690:three_prime_UTR 30.0%
! CTCCATAAAAGAAAAAGTGT+TGG - chr4_4:2595705-2595724 Msa0663700:CDS 30.0%
!!! CACCAACACTTTTTCTTTTA+TGG + chr4_4:2595710-2595729 Msa0663690:three_prime_UTR 30.0%
GGAAGTTTCCATAGAAAAAT+TGG + chr4_4:2595800-2595819 Msa0663690:three_prime_UTR 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG + chr4_4:2595861-2595880 Msa0663690:intron 30.0%
TTGTAGATATTCTGACATAG+AGG - chr4_4:2595916-2595935 Msa0663700:CDS 30.0%
GTTAGAATAGATTACGCTAT+AGG - chr4_4:2595938-2595957 Msa0663700:CDS 30.0%
AAAGCATGTAAAGAAGTTGA+GGG - chr4_4:2596085-2596104 Msa0663700:intron 30.0%
!! GATAACTCAGCTTTTAACTA+TGG - chr4_4:2596220-2596239 Msa0663700:intron 30.0%
AAATTTGGGGAAAACTTCTA+TGG + chr4_4:2596284-2596303 Msa0663690:intron 30.0%
CCATAGACAGTAACAAAATT+TGG + chr4_4:2596299-2596318 Msa0663690:intron 30.0%
! CCAAATTTTGTTACTGTCTA+TGG - chr4_4:2596296-2596315 Msa0663700:intron 30.0%
! AATTTTGTTACTGTCTATGG+TGG - chr4_4:2596299-2596318 Msa0663700:intron 30.0%
! ATTTTGTTACTGTCTATGGT+GGG - chr4_4:2596300-2596319 Msa0663700:intron 30.0%
ATGGCTAATGTGAAAAACAT+AGG + chr4_4:2596469-2596488 Msa0663690:three_prime_UTR 30.0%
TCTGATCTTCATATCAAAGT+CGG - chr4_4:2596517-2596536 Msa0663700:CDS 30.0%
ATTGTAGATTCACTTGATGT+TGG - chr4_4:2596580-2596599 Msa0663700:CDS 30.0%
TTGTAGATTCACTTGATGTT+GGG - chr4_4:2596581-2596600 Msa0663700:CDS 30.0%
AAGCAAAACAGAAGGATATT+TGG + chr4_4:2596695-2596714 None:intergenic 30.0%
CCATAGTAATAGCTATTATG+GGG - chr4_4:2596775-2596794 Msa0663700:intron 30.0%
CCAATTGCATTTATAACACA+AGG + chr4_4:2596905-2596924 None:intergenic 30.0%
CCTTGTGTTATAAATGCAAT+TGG - chr4_4:2596902-2596921 Msa0663700:intron 30.0%
GTCTTCTCTGTATATAAACT+CGG + chr4_4:2597345-2597364 None:intergenic 30.0%
AGAGAAGACTTACATAGTTA+GGG - chr4_4:2597356-2597375 Msa0663700:intron 30.0%
ATATTACGAATCTGATTACC+CGG - chr4_4:2597773-2597792 Msa0663700:CDS 30.0%
ATTGGAAGATCACTCAAAAG+TGG - chr4_4:2594897-2594916 Msa0663700:CDS 35.0%
!! TGTGACACTTTAAGATCTTC+TGG + chr4_4:2594942-2594961 Msa0663690:three_prime_UTR 35.0%
TGGCTAAACAATACGATTTG+TGG + chr4_4:2595082-2595101 Msa0663690:three_prime_UTR 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG + chr4_4:2595102-2595121 Msa0663690:three_prime_UTR 35.0%
CAAAACATATTGAGCATGGA+TGG + chr4_4:2595333-2595352 Msa0663690:three_prime_UTR 35.0%
TACGCAAAACATATTGAGCA+TGG + chr4_4:2595337-2595356 Msa0663690:three_prime_UTR 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG - chr4_4:2595407-2595426 Msa0663700:CDS 35.0%
! CATGCAATATTTTCCGAGAA+TGG - chr4_4:2595498-2595517 Msa0663700:intron 35.0%
TCAGAAGTTAAAAAGACAGC+TGG - chr4_4:2595541-2595560 Msa0663700:intron 35.0%
CATAAGTGATACAGAAGCAA+TGG + chr4_4:2595674-2595693 Msa0663690:three_prime_UTR 35.0%
TCTGTATCACTTATGAAGCT+CGG - chr4_4:2595679-2595698 Msa0663700:CDS 35.0%
! GAAGTATCAAGAAGTGTATG+AGG + chr4_4:2595839-2595858 Msa0663690:intron 35.0%
AGAATAGATTACGCTATAGG+CGG - chr4_4:2595941-2595960 Msa0663700:CDS 35.0%
GAAACATTGATCATTCGTGT+CGG + chr4_4:2595987-2596006 Msa0663690:three_prime_UTR 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG - chr4_4:2596084-2596103 Msa0663700:intron 35.0%
AGTTCAAGTAAGCATATACC+AGG - chr4_4:2596136-2596155 Msa0663700:intron 35.0%
GTTCAAGTAAGCATATACCA+GGG - chr4_4:2596137-2596156 Msa0663700:intron 35.0%
! TTTTGTTACTGTCTATGGTG+GGG - chr4_4:2596301-2596320 Msa0663700:intron 35.0%
TGTTATAAAGAGGACTCATG+TGG + chr4_4:2596380-2596399 Msa0663690:intron 35.0%
! TTTTCACATTAGCCATGTAG+AGG - chr4_4:2596473-2596492 Msa0663700:CDS 35.0%
ACTGTGAAAAGCAAAACAGA+AGG + chr4_4:2596703-2596722 None:intergenic 35.0%
!! TTTGCTTTTCACAGTACACT+TGG - chr4_4:2596708-2596727 Msa0663700:intron 35.0%
CCCCATAATAGCTATTACTA+TGG + chr4_4:2596778-2596797 None:intergenic 35.0%
TGCAATGAGAAATCTCAGAA+AGG + chr4_4:2597194-2597213 None:intergenic 35.0%
CAGAGAAGACTTACATAGTT+AGG - chr4_4:2597355-2597374 Msa0663700:intron 35.0%
AAGACTTACATAGTTAGGGA+AGG - chr4_4:2597360-2597379 Msa0663700:intron 35.0%
ATGAACAACATCTCATCAAC+TGG + chr4_4:2597390-2597409 None:intergenic 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG - chr4_4:2597477-2597496 Msa0663700:CDS 35.0%
TAGACACATACATAGTAGAC+AGG - chr4_4:2597638-2597657 Msa0663700:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG + chr4_4:2597677-2597696 None:intergenic 35.0%
CAATCTATATTCAAGCTGCA+TGG - chr4_4:2597709-2597728 Msa0663700:CDS 35.0%
TATTACGAATCTGATTACCC+GGG - chr4_4:2597774-2597793 Msa0663700:CDS 35.0%
!! CCAAATTTTGACGAACTTTG+AGG + chr4_4:2597834-2597853 None:intergenic 35.0%
CCTCAAAGTTCGTCAAAATT+TGG - chr4_4:2597831-2597850 Msa0663700:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG - chr4_4:2597859-2597878 Msa0663700:CDS 35.0%
! TGTTTGCCTTTTCCTATTCC+CGG - chr4_4:2594804-2594823 Msa0663700:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG + chr4_4:2594830-2594849 Msa0663690:three_prime_UTR 40.0%
! TCGTCGAAATCATTTCGAGT+CGG - chr4_4:2594843-2594862 Msa0663700:CDS 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG - chr4_4:2594854-2594873 Msa0663700:CDS 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG - chr4_4:2594855-2594874 Msa0663700:CDS 40.0%
ACTCAAAAGTGGAGTCACTT+GGG - chr4_4:2594908-2594927 Msa0663700:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG + chr4_4:2595206-2595225 Msa0663690:three_prime_UTR 40.0%
TCATCTCGAGTATTTGGAAG+AGG - chr4_4:2595206-2595225 Msa0663700:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG - chr4_4:2595410-2595429 Msa0663700:CDS 40.0%
AATGGTACGGTTCATACCTT+TGG - chr4_4:2595516-2595535 Msa0663700:intron 40.0%
GTGCCATGTACTATTTGATC+TGG - chr4_4:2595590-2595609 Msa0663700:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG - chr4_4:2595844-2595863 Msa0663700:intron 40.0%
ATATACCAGGGTATGACAGT+TGG - chr4_4:2596149-2596168 Msa0663700:intron 40.0%
GAAGATCAGACACACGATAT+GGG + chr4_4:2596507-2596526 Msa0663690:three_prime_UTR 40.0%
TGAAGATCAGACACACGATA+TGG + chr4_4:2596508-2596527 Msa0663690:three_prime_UTR 40.0%
! TTTTCACAGTACACTTGGTC+AGG - chr4_4:2596713-2596732 Msa0663700:intron 40.0%
TTTCACAGTACACTTGGTCA+GGG - chr4_4:2596714-2596733 Msa0663700:intron 40.0%
TGGAAAACAAAACCTCTCCA+GGG + chr4_4:2596758-2596777 None:intergenic 40.0%
ATGGAAAACAAAACCTCTCC+AGG + chr4_4:2596759-2596778 None:intergenic 40.0%
TCTTCTGATTGGAAACATGC+AGG - chr4_4:2596812-2596831 Msa0663700:intron 40.0%
GACCTACCTTCAATCAATGT+CGG - chr4_4:2596928-2596947 Msa0663700:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG + chr4_4:2596933-2596952 None:intergenic 40.0%
TGGAGGAAATGAGGATCAAA+AGG - chr4_4:2596957-2596976 Msa0663700:intron 40.0%
! AATTTTCTTCATCGACCCCT+CGG + chr4_4:2597075-2597094 None:intergenic 40.0%
AGGGGTCGATGAAGAAAATT+TGG - chr4_4:2597075-2597094 Msa0663700:intron 40.0%
GAGATGTTGTTCATCATTCG+TGG - chr4_4:2597396-2597415 Msa0663700:intron 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG - chr4_4:2597423-2597442 Msa0663700:intron 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG - chr4_4:2597461-2597480 Msa0663700:CDS 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG - chr4_4:2597465-2597484 Msa0663700:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG - chr4_4:2597497-2597516 Msa0663700:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG + chr4_4:2597552-2597571 None:intergenic 40.0%
TACCTCACTCATAGCTTTCA+CGG + chr4_4:2597568-2597587 None:intergenic 40.0%
TCCGTTTCTATTCGCAACAA+TGG - chr4_4:2597673-2597692 Msa0663700:CDS 40.0%
ATTCGCAACAATGGAGAACA+TGG - chr4_4:2597682-2597701 Msa0663700:CDS 40.0%
TTCGCAACAATGGAGAACAT+GGG - chr4_4:2597683-2597702 Msa0663700:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG - chr4_4:2597712-2597731 Msa0663700:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG + chr4_4:2597963-2597982 None:intergenic 40.0%
TTCATCCCACTTGAGCTAGA+TGG + chr4_4:2594762-2594781 Msa0663690:three_prime_UTR 45.0%
GGAGTGAAAAAGGGATCAGA+TGG - chr4_4:2594864-2594883 Msa0663700:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG - chr4_4:2594907-2594926 Msa0663700:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG - chr4_4:2594909-2594928 Msa0663700:CDS 45.0%
! AGATGAAGGTGCAATGAAGG+CGG + chr4_4:2595192-2595211 Msa0663690:three_prime_UTR 45.0%
! TCGAGATGAAGGTGCAATGA+AGG + chr4_4:2595195-2595214 Msa0663690:three_prime_UTR 45.0%
GCACCTTCATCTCGAGTATT+TGG - chr4_4:2595200-2595219 Msa0663700:CDS 45.0%
GGTGAACTTGTCATAGATCC+TGG - chr4_4:2595227-2595246 Msa0663700:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG + chr4_4:2595248-2595267 Msa0663690:three_prime_UTR 45.0%
TGGATGGTATCTCAAACCTG+TGG + chr4_4:2595317-2595336 Msa0663690:three_prime_UTR 45.0%
TCTTTATAGCGCAAGACGCA+TGG - chr4_4:2595435-2595454 Msa0663700:CDS 45.0%
GGTATGAACCGTACCATTCT+CGG + chr4_4:2595514-2595533 Msa0663690:intron 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG - chr4_4:2596023-2596042 Msa0663700:intron 45.0%
TAGAACGTAACGACACATGC+TGG - chr4_4:2596059-2596078 Msa0663700:intron 45.0%
TTTCTCCAACTGTCATACCC+TGG + chr4_4:2596157-2596176 Msa0663690:intron 45.0%
GGTGGGGACTTCAGAATTTA+AGG - chr4_4:2596317-2596336 Msa0663700:intron 45.0%
TTCACAGTACACTTGGTCAG+GGG - chr4_4:2596715-2596734 Msa0663700:intron 45.0%
AAACAAAACCTCTCCAGGGT+AGG + chr4_4:2596754-2596773 None:intergenic 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG - chr4_4:2596801-2596820 Msa0663700:intron 45.0%
! GACGAACCGACATTGATTGA+AGG + chr4_4:2596937-2596956 None:intergenic 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG - chr4_4:2596937-2596956 Msa0663700:intron 45.0%
GGGTGTTCAACCACTTGTAT+TGG + chr4_4:2597054-2597073 None:intergenic 45.0%
! ATTTTCTTCATCGACCCCTC+GGG + chr4_4:2597074-2597093 None:intergenic 45.0%
GGTTCCTCTCTTTGCTAACA+TGG - chr4_4:2597281-2597300 Msa0663700:CDS 45.0%
TCGTCCATGTTAGCAAAGAG+AGG + chr4_4:2597288-2597307 None:intergenic 45.0%
!! GAGAGTGTTACTACAGATGG+TGG - chr4_4:2597426-2597445 Msa0663700:intron 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG - chr4_4:2597435-2597454 Msa0663700:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG - chr4_4:2597450-2597469 Msa0663700:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG - chr4_4:2597496-2597515 Msa0663700:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG - chr4_4:2597503-2597522 Msa0663700:CDS 45.0%
AACCGTGAAAGCTATGAGTG+AGG - chr4_4:2597563-2597582 Msa0663700:CDS 45.0%
GAAAGCTATGAGTGAGGTAG+AGG - chr4_4:2597569-2597588 Msa0663700:CDS 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG - chr4_4:2597590-2597609 Msa0663700:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG - chr4_4:2597724-2597743 Msa0663700:CDS 45.0%
TTGCAGAATCATCACTTCCC+GGG + chr4_4:2597794-2597813 None:intergenic 45.0%
CTTGCAGAATCATCACTTCC+CGG + chr4_4:2597795-2597814 None:intergenic 45.0%
GGGAAGTGATGATTCTGCAA+GGG - chr4_4:2597794-2597813 Msa0663700:CDS 45.0%
! TTTGACGAACTTTGAGGACC+CGG + chr4_4:2597828-2597847 None:intergenic 45.0%
AGTACCAAGTTCAAGAGAGC+TGG - chr4_4:2597914-2597933 Msa0663700:CDS 45.0%
TTCACCAGCTCTCTTGAACT+TGG + chr4_4:2597921-2597940 None:intergenic 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG + chr4_4:2597947-2597966 None:intergenic 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG + chr4_4:2597948-2597967 None:intergenic 45.0%
! GCACTGAAATCAGGTTCTGA+GGG + chr4_4:2597954-2597973 None:intergenic 45.0%
! AGCACTGAAATCAGGTTCTG+AGG + chr4_4:2597955-2597974 None:intergenic 45.0%
!!! AATTGAATATAAATAAATTT+TGG + chr4_4:2595873-2595892 Msa0663690:intron 5.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG + chr4_4:2594825-2594844 Msa0663690:three_prime_UTR 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG + chr4_4:2594826-2594845 Msa0663690:three_prime_UTR 50.0%
GTATCTCAAACCTGTGGCAG+TGG + chr4_4:2595311-2595330 Msa0663690:three_prime_UTR 50.0%
TTATAGCGCAAGACGCATGG+AGG - chr4_4:2595438-2595457 Msa0663700:CDS 50.0%
CTTCTGACTAGTACCTACCC+TGG - chr4_4:2596738-2596757 Msa0663700:intron 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG - chr4_4:2596940-2596959 Msa0663700:intron 50.0%
CTGACTCTTTCCCTTAGGTC+CGG + chr4_4:2597022-2597041 None:intergenic 50.0%
AGCGTCTGACTCTTTCCCTT+AGG + chr4_4:2597027-2597046 None:intergenic 50.0%
GACGCTACGACCAATACAAG+TGG - chr4_4:2597041-2597060 Msa0663700:intron 50.0%
TACAAGTGGTTGAACACCCG+AGG - chr4_4:2597055-2597074 Msa0663700:intron 50.0%
ACAAGTGGTTGAACACCCGA+GGG - chr4_4:2597056-2597075 Msa0663700:intron 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG + chr4_4:2597531-2597550 None:intergenic 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG - chr4_4:2597730-2597749 Msa0663700:CDS 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG - chr4_4:2597736-2597755 Msa0663700:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG - chr4_4:2597740-2597759 Msa0663700:CDS 50.0%
CGGGAAGTGATGATTCTGCA+AGG - chr4_4:2597793-2597812 Msa0663700:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG - chr4_4:2597800-2597819 Msa0663700:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG - chr4_4:2597879-2597898 Msa0663700:CDS 50.0%
CAATGAAGGCGGTGCGGAAT+TGG + chr4_4:2595181-2595200 Msa0663690:three_prime_UTR 55.0%
AAGGTGCAATGAAGGCGGTG+CGG + chr4_4:2595187-2595206 Msa0663690:three_prime_UTR 55.0%
! CGCAAGACGCATGGAGGTAT+TGG - chr4_4:2595444-2595463 Msa0663700:CDS 55.0%
TGGGCACATGCACCTCTACA+TGG + chr4_4:2596488-2596507 Msa0663690:three_prime_UTR 55.0%
GACTAGTACCTACCCTGGAG+AGG - chr4_4:2596743-2596762 Msa0663700:intron 55.0%
CGGTTCGTCTGGAGGAAATG+AGG - chr4_4:2596948-2596967 Msa0663700:intron 55.0%
GCGATGATGCATCCACTGCT+CGG + chr4_4:2596990-2597009 None:intergenic 55.0%
GCATCATCGCTTGCTTCCTC+CGG - chr4_4:2597000-2597019 Msa0663700:intron 55.0%
CTTGCTTCCTCCGGACCTAA+GGG - chr4_4:2597009-2597028 Msa0663700:intron 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG + chr4_4:2597019-2597038 None:intergenic 55.0%
CAAGTGGTTGAACACCCGAG+GGG - chr4_4:2597057-2597076 Msa0663700:intron 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG - chr4_4:2597806-2597825 Msa0663700:CDS 55.0%
! CCTTTTCCTATTCCCGGCCG+CGG - chr4_4:2594810-2594829 Msa0663700:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG - chr4_4:2594811-2594830 Msa0663700:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG + chr4_4:2594819-2594838 Msa0663690:three_prime_UTR 60.0%
GTCTGTACTGCCACTGCCAC+AGG - chr4_4:2595298-2595317 Msa0663700:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG - chr4_4:2596975-2596994 Msa0663700:intron 60.0%
GCTTGCTTCCTCCGGACCTA+AGG - chr4_4:2597008-2597027 Msa0663700:intron 60.0%
AAGGCGAAGGCGAAGGAAGG+AGG - chr4_4:2597743-2597762 Msa0663700:CDS 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG - chr4_4:2597807-2597826 Msa0663700:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG + chr4_4:2594813-2594832 Msa0663690:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 2594756 2597992 2594756 ID=Msa0663700;Name=Msa0663700
chr4_4 mRNA 2594756 2597992 2594756 ID=Msa0663700-mRNA-1;Parent=Msa0663700;Name=Msa0663700-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|6|0|715
chr4_4 exon 2597429 2597992 2597429 ID=Msa0663700-mRNA-1:exon:774;Parent=Msa0663700-mRNA-1
chr4_4 exon 2597118 2597330 2597118 ID=Msa0663700-mRNA-1:exon:773;Parent=Msa0663700-mRNA-1
chr4_4 exon 2596410 2596720 2596410 ID=Msa0663700-mRNA-1:exon:772;Parent=Msa0663700-mRNA-1
chr4_4 exon 2595915 2596026 2595915 ID=Msa0663700-mRNA-1:exon:771;Parent=Msa0663700-mRNA-1
chr4_4 exon 2595583 2595819 2595583 ID=Msa0663700-mRNA-1:exon:770;Parent=Msa0663700-mRNA-1
chr4_4 exon 2594756 2595466 2594756 ID=Msa0663700-mRNA-1:exon:769;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2597429 2597992 2597429 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2597118 2597330 2597118 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2596410 2596720 2596410 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2595915 2596026 2595915 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2595583 2595819 2595583 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
chr4_4 CDS 2594756 2595466 2594756 ID=Msa0663700-mRNA-1:cds;Parent=Msa0663700-mRNA-1
Gene Sequence

>Msa0663700

ATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCACAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>Msa0663700

MPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE*