Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0542430 | XP_013443114.1 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0542430 | sp|Q9SZM1|EXP20_ARATH | 65.714 | 245 | 80 | 2 | 9 | 251 | 12 | 254 | 1.15e-118 | 342 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0542430 | I3SE17 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042640 | Msa0542430 | 0.817465 | 3.252139e-52 | -8.615850e-47 |
| Msa0089460 | Msa0542430 | 0.821453 | 4.004495e-53 | -8.615850e-47 |
| Msa0102240 | Msa0542430 | 0.802730 | 4.882910e-49 | -8.615850e-47 |
| Msa0542430 | Msa0662980 | 0.983144 | 6.011600e-157 | -8.615850e-47 |
| Msa0542430 | Msa1409410 | 0.810708 | 1.007751e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0542430 | MtrunA17_Chr4g0021561 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Msa0542430 | MtrunA17_Chr5g0433841 | 59.735 | 226 | 90 | 1 | 26 | 251 | 40 | 264 | 1.85e-97 | 286 |
| Msa0542430 | MtrunA17_Chr2g0324191 | 75.000 | 152 | 37 | 1 | 1 | 151 | 1 | 152 | 3.46e-87 | 255 |
| Msa0542430 | MtrunA17_Chr7g0238681 | 50.000 | 230 | 115 | 0 | 22 | 251 | 27 | 256 | 8.12e-87 | 258 |
| Msa0542430 | MtrunA17_Chr5g0435311 | 50.870 | 230 | 113 | 0 | 22 | 251 | 77 | 306 | 1.52e-86 | 259 |
| Msa0542430 | MtrunA17_Chr5g0432611 | 48.594 | 249 | 126 | 1 | 3 | 251 | 5 | 251 | 2.78e-86 | 256 |
| Msa0542430 | MtrunA17_Chr2g0328261 | 50.000 | 230 | 115 | 0 | 22 | 251 | 29 | 258 | 8.56e-86 | 256 |
| Msa0542430 | MtrunA17_Chr5g0417701 | 50.000 | 244 | 120 | 1 | 8 | 251 | 6 | 247 | 2.72e-85 | 254 |
| Msa0542430 | MtrunA17_Chr3g0128801 | 49.593 | 246 | 122 | 1 | 6 | 251 | 10 | 253 | 2.72e-85 | 254 |
| Msa0542430 | MtrunA17_Chr4g0042521 | 50.410 | 244 | 119 | 1 | 8 | 251 | 6 | 247 | 2.87e-85 | 254 |
| Msa0542430 | MtrunA17_Chr3g0109801 | 55.508 | 236 | 92 | 6 | 26 | 251 | 52 | 284 | 3.46e-85 | 255 |
| Msa0542430 | MtrunA17_Chr3g0092071 | 52.632 | 228 | 106 | 1 | 24 | 251 | 22 | 247 | 5.84e-85 | 253 |
| Msa0542430 | MtrunA17_Chr7g0218751 | 49.794 | 243 | 117 | 2 | 7 | 249 | 11 | 248 | 2.47e-84 | 252 |
| Msa0542430 | MtrunA17_Chr3g0128791 | 52.444 | 225 | 105 | 1 | 27 | 251 | 31 | 253 | 2.66e-84 | 252 |
| Msa0542430 | MtrunA17_Chr4g0031621 | 48.047 | 256 | 125 | 2 | 4 | 251 | 1 | 256 | 1.05e-83 | 250 |
| Msa0542430 | MtrunA17_Chr3g0107311 | 53.333 | 225 | 103 | 1 | 27 | 251 | 32 | 254 | 4.43e-83 | 249 |
| Msa0542430 | MtrunA17_Chr3g0107291 | 52.889 | 225 | 104 | 1 | 27 | 251 | 38 | 260 | 3.08e-82 | 247 |
| Msa0542430 | MtrunA17_Chr7g0217661 | 48.387 | 248 | 113 | 3 | 9 | 251 | 3 | 240 | 5.68e-80 | 240 |
| Msa0542430 | MtrunA17_Chr8g0360041 | 50.667 | 225 | 109 | 1 | 24 | 248 | 28 | 250 | 4.11e-79 | 238 |
| Msa0542430 | MtrunA17_Chr7g0218741 | 51.316 | 228 | 108 | 2 | 24 | 250 | 26 | 251 | 1.22e-77 | 235 |
| Msa0542430 | MtrunA17_Chr4g0069711 | 50.222 | 225 | 109 | 2 | 27 | 250 | 61 | 283 | 4.19e-76 | 232 |
| Msa0542430 | MtrunA17_Chr7g0218761 | 52.195 | 205 | 96 | 1 | 45 | 249 | 7 | 209 | 7.68e-76 | 229 |
| Msa0542430 | MtrunA17_Chr1g0193631 | 48.927 | 233 | 115 | 3 | 20 | 249 | 23 | 254 | 2.63e-75 | 229 |
| Msa0542430 | MtrunA17_Chr7g0271001 | 46.154 | 247 | 125 | 4 | 8 | 247 | 4 | 249 | 4.11e-74 | 226 |
| Msa0542430 | MtrunA17_Chr6g0453341 | 48.606 | 251 | 114 | 3 | 6 | 251 | 12 | 252 | 1.57e-72 | 222 |
| Msa0542430 | MtrunA17_Chr8g0360721 | 51.724 | 203 | 96 | 1 | 45 | 247 | 16 | 216 | 2.19e-72 | 220 |
| Msa0542430 | MtrunA17_Chr5g0398271 | 41.532 | 248 | 143 | 2 | 3 | 249 | 13 | 259 | 1.69e-67 | 209 |
| Msa0542430 | MtrunA17_Chr4g0057091 | 43.983 | 241 | 133 | 2 | 8 | 247 | 17 | 256 | 3.47e-67 | 208 |
| Msa0542430 | MtrunA17_Chr1g0157061 | 44.037 | 218 | 117 | 3 | 34 | 249 | 24 | 238 | 6.48e-66 | 204 |
| Msa0542430 | MtrunA17_Chr2g0324181 | 85.149 | 101 | 15 | 0 | 151 | 251 | 17 | 117 | 4.45e-61 | 188 |
| Msa0542430 | MtrunA17_Chr4g0022841 | 51.304 | 115 | 56 | 0 | 134 | 248 | 2 | 116 | 1.91e-36 | 125 |
| Msa0542430 | MtrunA17_Chr2g0324221 | 31.020 | 245 | 141 | 9 | 18 | 249 | 26 | 255 | 3.78e-29 | 110 |
| Msa0542430 | MtrunA17_Chr4g0022851 | 43.443 | 122 | 65 | 2 | 3 | 124 | 8 | 125 | 1.65e-28 | 105 |
| Msa0542430 | MtrunA17_Chr4g0021641 | 32.353 | 238 | 135 | 9 | 24 | 249 | 38 | 261 | 1.04e-27 | 107 |
| Msa0542430 | MtrunA17_Chr2g0324201 | 30.544 | 239 | 138 | 10 | 24 | 249 | 30 | 253 | 3.71e-27 | 105 |
| Msa0542430 | MtrunA17_Chr4g0012801 | 31.439 | 264 | 145 | 12 | 3 | 247 | 13 | 259 | 9.96e-25 | 99.0 |
| Msa0542430 | MtrunA17_Chr8g0359971 | 53.947 | 76 | 34 | 1 | 173 | 247 | 2 | 77 | 9.62e-22 | 86.3 |
| Msa0542430 | MtrunA17_Chr8g0359981 | 39.683 | 126 | 49 | 5 | 68 | 190 | 1 | 102 | 1.51e-20 | 84.0 |
| Msa0542430 | MtrunA17_Chr7g0231221 | 29.004 | 231 | 136 | 11 | 32 | 247 | 53 | 270 | 7.54e-19 | 83.6 |
| Msa0542430 | MtrunA17_Chr5g0399811 | 28.194 | 227 | 146 | 9 | 30 | 249 | 32 | 248 | 1.34e-13 | 68.6 |
| Msa0542430 | MtrunA17_Chr4g0055601 | 26.336 | 262 | 155 | 10 | 5 | 249 | 8 | 248 | 1.80e-13 | 68.2 |
| Msa0542430 | MtrunA17_Chr4g0038741 | 26.106 | 226 | 132 | 10 | 38 | 247 | 32 | 238 | 3.15e-12 | 64.7 |
| Msa0542430 | MtrunA17_Chr1g0193571 | 49.091 | 55 | 28 | 0 | 195 | 249 | 3 | 57 | 2.12e-11 | 58.2 |
| Msa0542430 | MtrunA17_Chr4g0055561 | 25.200 | 250 | 168 | 9 | 5 | 247 | 8 | 245 | 3.34e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0542430 | AT4G38210.1 | 65.714 | 245 | 80 | 2 | 9 | 251 | 12 | 254 | 1.17e-119 | 342 |
| Msa0542430 | AT3G03220.1 | 57.080 | 226 | 96 | 1 | 26 | 251 | 39 | 263 | 9.95e-93 | 274 |
| Msa0542430 | AT3G55500.1 | 50.201 | 249 | 120 | 1 | 7 | 251 | 10 | 258 | 1.79e-88 | 263 |
| Msa0542430 | AT2G39700.1 | 47.809 | 251 | 131 | 0 | 1 | 251 | 5 | 255 | 4.95e-88 | 261 |
| Msa0542430 | AT5G02260.1 | 47.200 | 250 | 127 | 1 | 7 | 251 | 7 | 256 | 2.38e-87 | 260 |
| Msa0542430 | AT2G37640.1 | 49.004 | 251 | 120 | 1 | 9 | 251 | 10 | 260 | 3.97e-87 | 259 |
| Msa0542430 | AT5G05290.1 | 51.271 | 236 | 112 | 2 | 17 | 251 | 22 | 255 | 1.44e-85 | 255 |
| Msa0542430 | AT2G40610.1 | 50.201 | 249 | 121 | 3 | 4 | 251 | 7 | 253 | 3.19e-84 | 252 |
| Msa0542430 | AT1G69530.2 | 50.202 | 247 | 119 | 2 | 7 | 251 | 5 | 249 | 5.94e-84 | 251 |
| Msa0542430 | AT1G69530.1 | 50.202 | 247 | 119 | 2 | 7 | 251 | 5 | 249 | 5.94e-84 | 251 |
| Msa0542430 | AT5G56320.1 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 7.06e-84 | 251 |
| Msa0542430 | AT5G56320.2 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 7.06e-84 | 251 |
| Msa0542430 | AT2G03090.1 | 52.632 | 228 | 106 | 1 | 24 | 251 | 27 | 252 | 1.45e-83 | 250 |
| Msa0542430 | AT2G37640.2 | 53.333 | 210 | 98 | 0 | 42 | 251 | 26 | 235 | 4.23e-83 | 248 |
| Msa0542430 | AT5G56320.3 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 4.56e-83 | 251 |
| Msa0542430 | AT1G69530.3 | 50.413 | 242 | 116 | 2 | 7 | 246 | 5 | 244 | 4.76e-82 | 247 |
| Msa0542430 | AT2G28950.1 | 46.825 | 252 | 133 | 1 | 1 | 251 | 4 | 255 | 4.34e-81 | 244 |
| Msa0542430 | AT5G39280.1 | 51.327 | 226 | 106 | 2 | 24 | 249 | 51 | 272 | 8.73e-79 | 239 |
| Msa0542430 | AT5G39300.1 | 50.442 | 226 | 108 | 2 | 24 | 249 | 52 | 273 | 1.41e-77 | 236 |
| Msa0542430 | AT5G39270.1 | 50.885 | 226 | 107 | 2 | 24 | 249 | 55 | 276 | 7.87e-77 | 234 |
| Msa0542430 | AT5G39290.1 | 50.442 | 226 | 108 | 2 | 24 | 249 | 55 | 276 | 3.31e-76 | 232 |
| Msa0542430 | AT1G26770.1 | 48.207 | 251 | 127 | 2 | 1 | 251 | 1 | 248 | 6.99e-76 | 231 |
| Msa0542430 | AT1G26770.2 | 48.207 | 251 | 127 | 2 | 1 | 251 | 11 | 258 | 9.11e-76 | 231 |
| Msa0542430 | AT3G29030.1 | 46.768 | 263 | 118 | 5 | 1 | 251 | 1 | 253 | 1.65e-75 | 230 |
| Msa0542430 | AT1G62980.1 | 43.621 | 243 | 135 | 2 | 8 | 249 | 12 | 253 | 1.15e-73 | 225 |
| Msa0542430 | AT4G01630.1 | 47.321 | 224 | 114 | 3 | 27 | 247 | 28 | 250 | 1.28e-70 | 217 |
| Msa0542430 | AT5G39260.1 | 43.922 | 255 | 128 | 4 | 1 | 249 | 14 | 259 | 5.10e-70 | 216 |
| Msa0542430 | AT5G39310.1 | 48.661 | 224 | 110 | 3 | 27 | 249 | 90 | 309 | 1.57e-69 | 216 |
| Msa0542430 | AT1G20190.1 | 43.966 | 232 | 125 | 3 | 19 | 247 | 18 | 247 | 3.71e-69 | 213 |
| Msa0542430 | AT1G69530.4 | 44.130 | 247 | 104 | 3 | 7 | 251 | 5 | 219 | 2.24e-68 | 210 |
| Msa0542430 | AT1G69530.5 | 49.510 | 204 | 99 | 2 | 7 | 208 | 5 | 206 | 4.19e-67 | 208 |
| Msa0542430 | AT1G12560.1 | 43.443 | 244 | 133 | 4 | 7 | 247 | 15 | 256 | 5.64e-67 | 208 |
| Msa0542430 | AT3G15370.1 | 43.969 | 257 | 132 | 6 | 1 | 251 | 1 | 251 | 2.63e-65 | 204 |
| Msa0542430 | AT3G15370.2 | 40.418 | 287 | 129 | 8 | 1 | 251 | 1 | 281 | 1.18e-60 | 193 |
| Msa0542430 | AT1G20190.2 | 46.032 | 189 | 97 | 3 | 62 | 247 | 2 | 188 | 6.96e-59 | 185 |
| Msa0542430 | AT4G28250.1 | 32.365 | 241 | 132 | 11 | 24 | 249 | 33 | 257 | 3.24e-24 | 98.2 |
| Msa0542430 | AT2G20750.1 | 33.068 | 251 | 140 | 11 | 13 | 249 | 28 | 264 | 1.95e-23 | 96.3 |
| Msa0542430 | AT1G65680.1 | 29.839 | 248 | 143 | 9 | 17 | 247 | 35 | 268 | 2.46e-23 | 95.9 |
| Msa0542430 | AT4G28250.2 | 32.083 | 240 | 128 | 11 | 24 | 249 | 33 | 251 | 3.36e-22 | 92.4 |
| Msa0542430 | AT2G45110.1 | 30.172 | 232 | 135 | 11 | 30 | 247 | 36 | 254 | 9.84e-18 | 80.5 |
| Msa0542430 | AT1G65681.1 | 28.636 | 220 | 132 | 9 | 40 | 247 | 11 | 217 | 4.56e-17 | 77.8 |
| Msa0542430 | AT3G60570.1 | 25.483 | 259 | 159 | 10 | 10 | 249 | 11 | 254 | 1.66e-14 | 71.6 |
| Msa0542430 | AT2G20750.2 | 32.804 | 189 | 103 | 8 | 13 | 190 | 28 | 203 | 3.78e-14 | 69.7 |
| Msa0542430 | AT4G17030.1 | 25.969 | 258 | 151 | 11 | 8 | 247 | 9 | 244 | 2.98e-11 | 62.4 |
| Msa0542430 | AT3G60570.2 | 25.792 | 221 | 136 | 9 | 42 | 249 | 1 | 206 | 3.30e-11 | 61.6 |
Find 74 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCTGTCCTCCTAATTT+TGG | 0.175976 | 4_1:+27507287 | Msa0542430:CDS |
| ATAAAATGAGTTGCTCTTCT+TGG | 0.221895 | 4_1:-27508382 | None:intergenic |
| GCACCTCCAAACTGGCAATT+TGG | 0.255098 | 4_1:+27508085 | Msa0542430:CDS |
| TAGAGATCCTTCTGTGTCTT+TGG | 0.259434 | 4_1:-27506885 | None:intergenic |
| TTTGGTCTCTCAGTTGATTA+TGG | 0.289936 | 4_1:+27507305 | Msa0542430:CDS |
| CTTATACAAATTATGATTCA+TGG | 0.302357 | 4_1:+27508177 | Msa0542430:three_prime_UTR |
| GGATACCAATGGCAAGGAAT+TGG | 0.318465 | 4_1:+27507966 | Msa0542430:CDS |
| GTTTGTCCAAATTGCCAGTT+TGG | 0.334344 | 4_1:-27508091 | None:intergenic |
| TGTCATTCATATATGTAAAC+AGG | 0.335548 | 4_1:+27508148 | Msa0542430:three_prime_UTR |
| TCAACTGAGAGACCAAAATT+AGG | 0.348503 | 4_1:-27507299 | None:intergenic |
| GATACCAATGGCAAGGAATT+GGG | 0.350848 | 4_1:+27507967 | Msa0542430:CDS |
| ACATCAGCCTTTGTCCTTTG+AGG | 0.354377 | 4_1:+27508021 | Msa0542430:CDS |
| CTAAACGCTGTTATCTAAGA+TGG | 0.361741 | 4_1:+27506763 | Msa0542430:exon |
| GACAACTCAAAATGTTCTCT+TGG | 0.372662 | 4_1:-27507344 | None:intergenic |
| GCAGAAGGAGCTTGTGGTTA+TGG | 0.372980 | 4_1:+27507107 | Msa0542430:intron |
| ACAGAAGGATCTCTAATTAC+AGG | 0.385780 | 4_1:+27506893 | Msa0542430:CDS |
| TTATTCCCGTTCAATATAGA+AGG | 0.389617 | 4_1:+27507408 | Msa0542430:CDS |
| CAATTTGGACAAACATTTGA+AGG | 0.398107 | 4_1:+27508100 | Msa0542430:CDS |
| TTGCTTACAAAGATCAAGAA+TGG | 0.419745 | 4_1:+27506840 | Msa0542430:CDS |
| CAAGGAATTGGGGAATGAAT+TGG | 0.429950 | 4_1:+27507978 | Msa0542430:CDS |
| ATCTACCTTCTATATTGAAC+GGG | 0.431748 | 4_1:-27507413 | None:intergenic |
| CAAGCATACACCACAAGATA+TGG | 0.436575 | 4_1:-27507235 | None:intergenic |
| ATAACTAATGTGGGGTTAGA+TGG | 0.442300 | 4_1:+27507902 | Msa0542430:CDS |
| ACAATGTAGCACCTCCAAAC+TGG | 0.451802 | 4_1:+27508077 | Msa0542430:CDS |
| CTTTGTGTTGTGTATACCAT+TGG | 0.466580 | 4_1:-27508310 | None:intergenic |
| GTTATATAAAATTGAGCCAA+TGG | 0.479724 | 4_1:+27508294 | Msa0542430:three_prime_UTR |
| GAGTAATTAAATTTGCATGA+TGG | 0.482311 | 4_1:-27508408 | None:intergenic |
| GAGGCAATGATGCTAGGAAA+AGG | 0.499306 | 4_1:-27506672 | None:intergenic |
| ATTCCCCAATTCCTTGCCAT+TGG | 0.501334 | 4_1:-27507971 | None:intergenic |
| AATTATGATTCATGGAATCA+TGG | 0.502165 | 4_1:+27508185 | Msa0542430:three_prime_UTR |
| AAAGTGAAGGGATCTAGAAC+AGG | 0.502893 | 4_1:+27507941 | Msa0542430:CDS |
| TGCATTTGCTGAAATTGCTA+AGG | 0.506123 | 4_1:+27507373 | Msa0542430:CDS |
| TCTCAGTTGATTATGGTGGT+TGG | 0.506701 | 4_1:+27507312 | Msa0542430:CDS |
| CAAGTTCTGATAACTAATGT+GGG | 0.508543 | 4_1:+27507893 | Msa0542430:CDS |
| TCAAGTTCTGATAACTAATG+TGG | 0.509065 | 4_1:+27507892 | Msa0542430:CDS |
| AGGACAAAGGCTGATGTTGA+AGG | 0.517191 | 4_1:-27508015 | None:intergenic |
| GATCTTCACAAAGCTAGCTA+TGG | 0.521811 | 4_1:+27507131 | Msa0542430:CDS |
| TAAACGCTGTTATCTAAGAT+GGG | 0.523848 | 4_1:+27506764 | Msa0542430:exon |
| CATATCTTGTGGTGTATGCT+TGG | 0.525681 | 4_1:+27507236 | Msa0542430:CDS |
| GTCTTTGGCATAAGTTGCAG+TGG | 0.528308 | 4_1:-27506870 | None:intergenic |
| TATCTACCTTCTATATTGAA+CGG | 0.536318 | 4_1:-27507414 | None:intergenic |
| TGTCCAAATTGCCAGTTTGG+AGG | 0.539604 | 4_1:-27508088 | None:intergenic |
| GTGAAGTGTGAAAGAAGTGG+AGG | 0.540916 | 4_1:+27507833 | Msa0542430:CDS |
| GCATTTGCTGAAATTGCTAA+GGG | 0.541056 | 4_1:+27507374 | Msa0542430:CDS |
| GAGGTATCCTGATATGTAGT+AGG | 0.545646 | 4_1:+27506635 | None:intergenic |
| TTTGAGGTAACTAGCAGCAC+TGG | 0.546125 | 4_1:+27508037 | Msa0542430:CDS |
| ATATGTGAGGCAATGATGCT+AGG | 0.552240 | 4_1:-27506678 | None:intergenic |
| AGGCAATGATGCTAGGAAAA+GGG | 0.552818 | 4_1:-27506671 | None:intergenic |
| TTCAACAGAGGGAGTACATG+TGG | 0.560849 | 4_1:+27507185 | Msa0542430:CDS |
| AGCTATGGAAAACATAGTGT+TGG | 0.560850 | 4_1:+27507146 | Msa0542430:CDS |
| GGGTTGAAATTCACAATGAG+TGG | 0.572612 | 4_1:+27507854 | Msa0542430:CDS |
| GGTCTCTCAGTTGATTATGG+TGG | 0.580447 | 4_1:+27507308 | Msa0542430:CDS |
| ATACCAATGGCAAGGAATTG+GGG | 0.587228 | 4_1:+27507968 | Msa0542430:CDS |
| TCAACAGAGGGAGTACATGT+GGG | 0.614475 | 4_1:+27507186 | Msa0542430:CDS |
| TAAAATCAAGTAGAGAAGTG+TGG | 0.616273 | 4_1:-27506798 | None:intergenic |
| TGAAGTGTGAAAGAAGTGGA+GGG | 0.622177 | 4_1:+27507834 | Msa0542430:CDS |
| TGAAGGGATCTAGAACAGGA+TGG | 0.625878 | 4_1:+27507945 | Msa0542430:CDS |
| ACTGAGAGACCAAAATTAGG+AGG | 0.626412 | 4_1:-27507296 | None:intergenic |
| CAGGATGGATACCAATGGCA+AGG | 0.630186 | 4_1:+27507960 | Msa0542430:CDS |
| CTAGTTACCTCAAAGGACAA+AGG | 0.637645 | 4_1:-27508028 | None:intergenic |
| GTCATTCATATATGTAAACA+GGG | 0.643903 | 4_1:+27508149 | Msa0542430:three_prime_UTR |
| GTGGCAGTAACAATAACAGA+AGG | 0.647537 | 4_1:-27507263 | None:intergenic |
| TAGAACAGGATGGATACCAA+TGG | 0.662207 | 4_1:+27507955 | Msa0542430:CDS |
| CAACAGAGGGAGTACATGTG+GGG | 0.668401 | 4_1:+27507187 | Msa0542430:CDS |
| ACTTATGCCAAAGACACAGA+AGG | 0.668875 | 4_1:+27506878 | Msa0542430:CDS |
| AGTGCTGCTAGTTACCTCAA+AGG | 0.674064 | 4_1:-27508035 | None:intergenic |
| CATTTGCTGAAATTGCTAAG+GGG | 0.677827 | 4_1:+27507375 | Msa0542430:CDS |
| TGATCAAAGAATTGTGCAAG+TGG | 0.686079 | 4_1:+27508241 | Msa0542430:three_prime_UTR |
| AAGTTCTGATAACTAATGTG+GGG | 0.688292 | 4_1:+27507894 | Msa0542430:CDS |
| ATTAGGAGGACAGAAATCAG+TGG | 0.688419 | 4_1:-27507282 | None:intergenic |
| TGTGATGCAGAAGGAGCTTG+TGG | 0.691735 | 4_1:+27507101 | Msa0542430:intron |
| AGAGTGAAGTGTGAAAGAAG+TGG | 0.695816 | 4_1:+27507830 | Msa0542430:intron |
| TCAAAGAATTGTGCAAGTGG+TGG | 0.708131 | 4_1:+27508244 | Msa0542430:three_prime_UTR |
| GATGTGTTGACCATATCTTG+TGG | 0.783191 | 4_1:+27507225 | Msa0542430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATGAAATAATTTACTTTT+AGG | - | chr4_1:27506719-27506738 | None:intergenic | 10.0% |
| !!! | ATTTTTTTATAAGTGTTTTT+GGG | + | chr4_1:27507438-27507457 | Msa0542430:intron | 10.0% |
| !!! | TCATGTACATTTTTTTTTTT+TGG | - | chr4_1:27507069-27507088 | None:intergenic | 15.0% |
| !!! | GATTTTTTTATAAGTGTTTT+TGG | + | chr4_1:27507437-27507456 | Msa0542430:intron | 15.0% |
| !! | AACTTAATTAATCTCTTATT+TGG | + | chr4_1:27507477-27507496 | Msa0542430:intron | 15.0% |
| !!! | TGTACATTTTTTTTTTTTGG+TGG | - | chr4_1:27507066-27507085 | None:intergenic | 20.0% |
| !! | GTTTATATAAAAACAGTTCA+AGG | - | chr4_1:27507562-27507581 | None:intergenic | 20.0% |
| !! | AAATGAAACTAAAAAACACA+AGG | - | chr4_1:27507629-27507648 | None:intergenic | 20.0% |
| !!! | TGTGTTTTTTAGTTTCATTT+TGG | + | chr4_1:27507629-27507648 | Msa0542430:intron | 20.0% |
| !! | ATAACATAGCTAATAAAAAG+AGG | - | chr4_1:27507711-27507730 | None:intergenic | 20.0% |
| !!! | ATTTGAAGGAAAACAATTTT+AGG | + | chr4_1:27508114-27508133 | Msa0542430:CDS | 20.0% |
| !! | CTTATACAAATTATGATTCA+TGG | + | chr4_1:27508177-27508196 | Msa0542430:three_prime_UTR | 20.0% |
| !!! | ACATTTTTTTTTTTTGGTGG+TGG | - | chr4_1:27507063-27507082 | None:intergenic | 25.0% |
| ! | TATCTACCTTCTATATTGAA+CGG | - | chr4_1:27507417-27507436 | None:intergenic | 25.0% |
| ! | AAAAAGAGGCAAAAAAGATT+AGG | - | chr4_1:27507697-27507716 | None:intergenic | 25.0% |
| ! | GCACAAACAAAACAAAAATT+TGG | - | chr4_1:27507799-27507818 | None:intergenic | 25.0% |
| ! | TGTCATTCATATATGTAAAC+AGG | + | chr4_1:27508148-27508167 | Msa0542430:three_prime_UTR | 25.0% |
| ! | GTCATTCATATATGTAAACA+GGG | + | chr4_1:27508149-27508168 | Msa0542430:three_prime_UTR | 25.0% |
| ! | AATTATGATTCATGGAATCA+TGG | + | chr4_1:27508185-27508204 | Msa0542430:three_prime_UTR | 25.0% |
| ! | GTTATATAAAATTGAGCCAA+TGG | + | chr4_1:27508294-27508313 | Msa0542430:three_prime_UTR | 25.0% |
| ! | TTGTGTTTTTCTCATATGTG+AGG | - | chr4_1:27506694-27506713 | None:intergenic | 30.0% |
| ! | TAAACGCTGTTATCTAAGAT+GGG | + | chr4_1:27506764-27506783 | Msa0542430:exon | 30.0% |
| TAAAATCAAGTAGAGAAGTG+TGG | - | chr4_1:27506801-27506820 | None:intergenic | 30.0% | |
| TTGCTTACAAAGATCAAGAA+TGG | + | chr4_1:27506840-27506859 | Msa0542430:CDS | 30.0% | |
| CATAATATATGCAAGATCCA+GGG | + | chr4_1:27507025-27507044 | Msa0542430:intron | 30.0% | |
| !!! | TTTTTTTTTTTGGTGGTGGT+CGG | - | chr4_1:27507059-27507078 | None:intergenic | 30.0% |
| TTATTCCCGTTCAATATAGA+AGG | + | chr4_1:27507408-27507427 | Msa0542430:CDS | 30.0% | |
| ATCTACCTTCTATATTGAAC+GGG | - | chr4_1:27507416-27507435 | None:intergenic | 30.0% | |
| !!! | AAGTGTTTTTGGGATGTTTT+TGG | + | chr4_1:27507448-27507467 | Msa0542430:intron | 30.0% |
| ! | GTTTTGCTCAATTACACATT+TGG | + | chr4_1:27507582-27507601 | Msa0542430:intron | 30.0% |
| ! | TCAAGTTCTGATAACTAATG+TGG | + | chr4_1:27507892-27507911 | Msa0542430:CDS | 30.0% |
| ! | CAAGTTCTGATAACTAATGT+GGG | + | chr4_1:27507893-27507912 | Msa0542430:CDS | 30.0% |
| ! | AAGTTCTGATAACTAATGTG+GGG | + | chr4_1:27507894-27507913 | Msa0542430:CDS | 30.0% |
| CAATTTGGACAAACATTTGA+AGG | + | chr4_1:27508100-27508119 | Msa0542430:CDS | 30.0% | |
| ! | AACAATTTTAGGCTTCAACA+TGG | + | chr4_1:27508125-27508144 | Msa0542430:exon | 30.0% |
| ! | ATGAGCAAATATACCGTTTT+TGG | - | chr4_1:27508215-27508234 | None:intergenic | 30.0% |
| ATAAAATGAGTTGCTCTTCT+TGG | - | chr4_1:27508385-27508404 | None:intergenic | 30.0% | |
| CTAAACGCTGTTATCTAAGA+TGG | + | chr4_1:27506763-27506782 | Msa0542430:exon | 35.0% | |
| ACAGAAGGATCTCTAATTAC+AGG | + | chr4_1:27506893-27506912 | Msa0542430:CDS | 35.0% | |
| ! | ATGTTGCTGTTATGAACCAA+AGG | - | chr4_1:27506935-27506954 | None:intergenic | 35.0% |
| GCATAATATATGCAAGATCC+AGG | + | chr4_1:27507024-27507043 | Msa0542430:intron | 35.0% | |
| !!! | TTTTTTTTTTGGTGGTGGTC+GGG | - | chr4_1:27507058-27507077 | None:intergenic | 35.0% |
| AGCTATGGAAAACATAGTGT+TGG | + | chr4_1:27507146-27507165 | Msa0542430:CDS | 35.0% | |
| ! | AGCACAATTTTGTTCAACAG+AGG | + | chr4_1:27507173-27507192 | Msa0542430:CDS | 35.0% |
| ! | GCACAATTTTGTTCAACAGA+GGG | + | chr4_1:27507174-27507193 | Msa0542430:CDS | 35.0% |
| ! | GATTTCTGTCCTCCTAATTT+TGG | + | chr4_1:27507287-27507306 | Msa0542430:CDS | 35.0% |
| TCAACTGAGAGACCAAAATT+AGG | - | chr4_1:27507302-27507321 | None:intergenic | 35.0% | |
| TTTGGTCTCTCAGTTGATTA+TGG | + | chr4_1:27507305-27507324 | Msa0542430:CDS | 35.0% | |
| GACAACTCAAAATGTTCTCT+TGG | - | chr4_1:27507347-27507366 | None:intergenic | 35.0% | |
| !!! | AGAACATTTTGAGTTGTCAC+TGG | + | chr4_1:27507349-27507368 | Msa0542430:CDS | 35.0% |
| ! | TGCATTTGCTGAAATTGCTA+AGG | + | chr4_1:27507373-27507392 | Msa0542430:CDS | 35.0% |
| ! | GCATTTGCTGAAATTGCTAA+GGG | + | chr4_1:27507374-27507393 | Msa0542430:CDS | 35.0% |
| ! | CATTTGCTGAAATTGCTAAG+GGG | + | chr4_1:27507375-27507394 | Msa0542430:CDS | 35.0% |
| ATAACTAATGTGGGGTTAGA+TGG | + | chr4_1:27507902-27507921 | Msa0542430:CDS | 35.0% | |
| !! | AGTTTTTGCTGTGAAAGTGA+AGG | + | chr4_1:27507928-27507947 | Msa0542430:CDS | 35.0% |
| !! | GTTTTTGCTGTGAAAGTGAA+GGG | + | chr4_1:27507929-27507948 | Msa0542430:CDS | 35.0% |
| GAATCATGGTAGACCAAAAA+CGG | + | chr4_1:27508199-27508218 | Msa0542430:three_prime_UTR | 35.0% | |
| TGATCAAAGAATTGTGCAAG+TGG | + | chr4_1:27508241-27508260 | Msa0542430:three_prime_UTR | 35.0% | |
| CTTTGTGTTGTGTATACCAT+TGG | - | chr4_1:27508313-27508332 | None:intergenic | 35.0% | |
| !!! | ATGACTGCTTTTTTGGTCAA+TGG | - | chr4_1:27508346-27508365 | None:intergenic | 35.0% |
| !!! | TTCGTCAATGACTGCTTTTT+TGG | - | chr4_1:27508353-27508372 | None:intergenic | 35.0% |
| AGGCAATGATGCTAGGAAAA+GGG | - | chr4_1:27506674-27506693 | None:intergenic | 40.0% | |
| ATATGTGAGGCAATGATGCT+AGG | - | chr4_1:27506681-27506700 | None:intergenic | 40.0% | |
| ACTTATGCCAAAGACACAGA+AGG | + | chr4_1:27506878-27506897 | Msa0542430:CDS | 40.0% | |
| TAGAGATCCTTCTGTGTCTT+TGG | - | chr4_1:27506888-27506907 | None:intergenic | 40.0% | |
| ! | TACTTTTCTTGCTCCTCCTT+TGG | + | chr4_1:27506916-27506935 | Msa0542430:intron | 40.0% |
| ! | TTGCTGTTATGAACCAAAGG+AGG | - | chr4_1:27506932-27506951 | None:intergenic | 40.0% |
| !!! | TTTTTTTTTGGTGGTGGTCG+GGG | - | chr4_1:27507057-27507076 | None:intergenic | 40.0% |
| GATCTTCACAAAGCTAGCTA+TGG | + | chr4_1:27507131-27507150 | Msa0542430:CDS | 40.0% | |
| ! | GATGTGTTGACCATATCTTG+TGG | + | chr4_1:27507225-27507244 | Msa0542430:CDS | 40.0% |
| CAAGCATACACCACAAGATA+TGG | - | chr4_1:27507238-27507257 | None:intergenic | 40.0% | |
| CATATCTTGTGGTGTATGCT+TGG | + | chr4_1:27507236-27507255 | Msa0542430:CDS | 40.0% | |
| GTGGCAGTAACAATAACAGA+AGG | - | chr4_1:27507266-27507285 | None:intergenic | 40.0% | |
| ATTAGGAGGACAGAAATCAG+TGG | - | chr4_1:27507285-27507304 | None:intergenic | 40.0% | |
| ACTGAGAGACCAAAATTAGG+AGG | - | chr4_1:27507299-27507318 | None:intergenic | 40.0% | |
| TCTCAGTTGATTATGGTGGT+TGG | + | chr4_1:27507312-27507331 | Msa0542430:CDS | 40.0% | |
| AGAGTGAAGTGTGAAAGAAG+TGG | + | chr4_1:27507830-27507849 | Msa0542430:intron | 40.0% | |
| TGAAGTGTGAAAGAAGTGGA+GGG | + | chr4_1:27507834-27507853 | Msa0542430:CDS | 40.0% | |
| GGGTTGAAATTCACAATGAG+TGG | + | chr4_1:27507854-27507873 | Msa0542430:CDS | 40.0% | |
| ! | AAAGTGAAGGGATCTAGAAC+AGG | + | chr4_1:27507941-27507960 | Msa0542430:CDS | 40.0% |
| TAGAACAGGATGGATACCAA+TGG | + | chr4_1:27507955-27507974 | Msa0542430:CDS | 40.0% | |
| GATACCAATGGCAAGGAATT+GGG | + | chr4_1:27507967-27507986 | Msa0542430:CDS | 40.0% | |
| ATACCAATGGCAAGGAATTG+GGG | + | chr4_1:27507968-27507987 | Msa0542430:CDS | 40.0% | |
| CAAGGAATTGGGGAATGAAT+TGG | + | chr4_1:27507978-27507997 | Msa0542430:CDS | 40.0% | |
| CTAGTTACCTCAAAGGACAA+AGG | - | chr4_1:27508031-27508050 | None:intergenic | 40.0% | |
| GTTTGTCCAAATTGCCAGTT+TGG | - | chr4_1:27508094-27508113 | None:intergenic | 40.0% | |
| TCAAAGAATTGTGCAAGTGG+TGG | + | chr4_1:27508244-27508263 | Msa0542430:three_prime_UTR | 40.0% | |
| GAGGCAATGATGCTAGGAAA+AGG | - | chr4_1:27506675-27506694 | None:intergenic | 45.0% | |
| GTCTTTGGCATAAGTTGCAG+TGG | - | chr4_1:27506873-27506892 | None:intergenic | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+TGG | + | chr4_1:27506996-27507015 | Msa0542430:intron | 45.0% |
| TTGTGTGCATGTGATGCAGA+AGG | + | chr4_1:27507092-27507111 | Msa0542430:intron | 45.0% | |
| ! | TTCAACAGAGGGAGTACATG+TGG | + | chr4_1:27507185-27507204 | Msa0542430:CDS | 45.0% |
| ! | TCAACAGAGGGAGTACATGT+GGG | + | chr4_1:27507186-27507205 | Msa0542430:CDS | 45.0% |
| GGTCTCTCAGTTGATTATGG+TGG | + | chr4_1:27507308-27507327 | Msa0542430:CDS | 45.0% | |
| GTGAAGTGTGAAAGAAGTGG+AGG | + | chr4_1:27507833-27507852 | Msa0542430:CDS | 45.0% | |
| ! | TGAAGGGATCTAGAACAGGA+TGG | + | chr4_1:27507945-27507964 | Msa0542430:CDS | 45.0% |
| GGATACCAATGGCAAGGAAT+TGG | + | chr4_1:27507966-27507985 | Msa0542430:CDS | 45.0% | |
| ATTCCCCAATTCCTTGCCAT+TGG | - | chr4_1:27507974-27507993 | None:intergenic | 45.0% | |
| AGGACAAAGGCTGATGTTGA+AGG | - | chr4_1:27508018-27508037 | None:intergenic | 45.0% | |
| ACATCAGCCTTTGTCCTTTG+AGG | + | chr4_1:27508021-27508040 | Msa0542430:CDS | 45.0% | |
| ! | AGTGCTGCTAGTTACCTCAA+AGG | - | chr4_1:27508038-27508057 | None:intergenic | 45.0% |
| ! | TTTGAGGTAACTAGCAGCAC+TGG | + | chr4_1:27508037-27508056 | Msa0542430:CDS | 45.0% |
| ACAATGTAGCACCTCCAAAC+TGG | + | chr4_1:27508077-27508096 | Msa0542430:CDS | 45.0% | |
| TGTCCAAATTGCCAGTTTGG+AGG | - | chr4_1:27508091-27508110 | None:intergenic | 45.0% | |
| TGTGATGCAGAAGGAGCTTG+TGG | + | chr4_1:27507101-27507120 | Msa0542430:intron | 50.0% | |
| GCAGAAGGAGCTTGTGGTTA+TGG | + | chr4_1:27507107-27507126 | Msa0542430:intron | 50.0% | |
| ! | CAACAGAGGGAGTACATGTG+GGG | + | chr4_1:27507187-27507206 | Msa0542430:CDS | 50.0% |
| CAGGATGGATACCAATGGCA+AGG | + | chr4_1:27507960-27507979 | Msa0542430:CDS | 50.0% | |
| GCACCTCCAAACTGGCAATT+TGG | + | chr4_1:27508085-27508104 | Msa0542430:CDS | 50.0% | |
| CCAACTGAGCTAAGCTCACG+AGG | - | chr4_1:27506994-27507013 | None:intergenic | 55.0% | |
| CCTCGTGAGCTTAGCTCAGT+TGG | + | chr4_1:27506991-27507010 | Msa0542430:intron | 55.0% | |
| GGTGGTCGGGGTTTGAACCC+TGG | - | chr4_1:27507045-27507064 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 27506645 | 27508422 | 27506645 | ID=Msa0542430;Name=Msa0542430 |
| chr4_1 | mRNA | 27506645 | 27508422 | 27506645 | ID=Msa0542430-mRNA-1;Parent=Msa0542430;Name=Msa0542430-mRNA-1;_AED=0.05;_eAED=0.05;_QI=137|1|1|1|1|1|3|287|251 |
| chr4_1 | exon | 27506645 | 27506914 | 27506645 | ID=Msa0542430-mRNA-1:exon:7396;Parent=Msa0542430-mRNA-1 |
| chr4_1 | exon | 27507111 | 27507429 | 27507111 | ID=Msa0542430-mRNA-1:exon:7397;Parent=Msa0542430-mRNA-1 |
| chr4_1 | exon | 27507832 | 27508422 | 27507832 | ID=Msa0542430-mRNA-1:exon:7398;Parent=Msa0542430-mRNA-1 |
| chr4_1 | five_prime_UTR | 27506645 | 27506781 | 27506645 | ID=Msa0542430-mRNA-1:five_prime_utr;Parent=Msa0542430-mRNA-1 |
| chr4_1 | CDS | 27506782 | 27506914 | 27506782 | ID=Msa0542430-mRNA-1:cds;Parent=Msa0542430-mRNA-1 |
| chr4_1 | CDS | 27507111 | 27507429 | 27507111 | ID=Msa0542430-mRNA-1:cds;Parent=Msa0542430-mRNA-1 |
| chr4_1 | CDS | 27507832 | 27508135 | 27507832 | ID=Msa0542430-mRNA-1:cds;Parent=Msa0542430-mRNA-1 |
| chr4_1 | three_prime_UTR | 27508136 | 27508422 | 27508136 | ID=Msa0542430-mRNA-1:three_prime_utr;Parent=Msa0542430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
