Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0662980 | XP_013443114.1 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0662980 | sp|Q9SZM1|EXP20_ARATH | 65.714 | 245 | 80 | 2 | 9 | 251 | 12 | 254 | 1.15e-118 | 342 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0662980 | I3SE17 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042640 | Msa0662980 | 0.818825 | 1.601069e-52 | -8.615850e-47 |
| Msa0077340 | Msa0662980 | 0.801293 | 9.642671e-49 | -8.615850e-47 |
| Msa0089460 | Msa0662980 | 0.815617 | 8.434107e-52 | -8.615850e-47 |
| Msa0102240 | Msa0662980 | 0.812137 | 4.932445e-51 | -8.615850e-47 |
| Msa0662980 | Msa0674480 | 0.808810 | 2.579416e-50 | -8.615850e-47 |
| Msa0662980 | Msa0734250 | 0.804937 | 1.699214e-49 | -8.615850e-47 |
| Msa0662980 | Msa0795750 | 0.809395 | 1.933086e-50 | -8.615850e-47 |
| Msa0662980 | Msa1409410 | 0.805152 | 1.532297e-49 | -8.615850e-47 |
| Msa0542430 | Msa0662980 | 0.983144 | 6.011600e-157 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0662980 | MtrunA17_Chr4g0021561 | 96.414 | 251 | 9 | 0 | 1 | 251 | 6 | 256 | 0.0 | 510 |
| Msa0662980 | MtrunA17_Chr5g0433841 | 59.735 | 226 | 90 | 1 | 26 | 251 | 40 | 264 | 1.85e-97 | 286 |
| Msa0662980 | MtrunA17_Chr2g0324191 | 75.000 | 152 | 37 | 1 | 1 | 151 | 1 | 152 | 3.46e-87 | 255 |
| Msa0662980 | MtrunA17_Chr7g0238681 | 50.000 | 230 | 115 | 0 | 22 | 251 | 27 | 256 | 8.12e-87 | 258 |
| Msa0662980 | MtrunA17_Chr5g0435311 | 50.870 | 230 | 113 | 0 | 22 | 251 | 77 | 306 | 1.52e-86 | 259 |
| Msa0662980 | MtrunA17_Chr5g0432611 | 48.594 | 249 | 126 | 1 | 3 | 251 | 5 | 251 | 2.78e-86 | 256 |
| Msa0662980 | MtrunA17_Chr2g0328261 | 50.000 | 230 | 115 | 0 | 22 | 251 | 29 | 258 | 8.56e-86 | 256 |
| Msa0662980 | MtrunA17_Chr5g0417701 | 50.000 | 244 | 120 | 1 | 8 | 251 | 6 | 247 | 2.72e-85 | 254 |
| Msa0662980 | MtrunA17_Chr3g0128801 | 49.593 | 246 | 122 | 1 | 6 | 251 | 10 | 253 | 2.72e-85 | 254 |
| Msa0662980 | MtrunA17_Chr4g0042521 | 50.410 | 244 | 119 | 1 | 8 | 251 | 6 | 247 | 2.87e-85 | 254 |
| Msa0662980 | MtrunA17_Chr3g0109801 | 55.508 | 236 | 92 | 6 | 26 | 251 | 52 | 284 | 3.46e-85 | 255 |
| Msa0662980 | MtrunA17_Chr3g0092071 | 52.632 | 228 | 106 | 1 | 24 | 251 | 22 | 247 | 5.84e-85 | 253 |
| Msa0662980 | MtrunA17_Chr7g0218751 | 49.794 | 243 | 117 | 2 | 7 | 249 | 11 | 248 | 2.47e-84 | 252 |
| Msa0662980 | MtrunA17_Chr3g0128791 | 52.444 | 225 | 105 | 1 | 27 | 251 | 31 | 253 | 2.66e-84 | 252 |
| Msa0662980 | MtrunA17_Chr4g0031621 | 48.047 | 256 | 125 | 2 | 4 | 251 | 1 | 256 | 1.05e-83 | 250 |
| Msa0662980 | MtrunA17_Chr3g0107311 | 53.333 | 225 | 103 | 1 | 27 | 251 | 32 | 254 | 4.43e-83 | 249 |
| Msa0662980 | MtrunA17_Chr3g0107291 | 52.889 | 225 | 104 | 1 | 27 | 251 | 38 | 260 | 3.08e-82 | 247 |
| Msa0662980 | MtrunA17_Chr7g0217661 | 48.387 | 248 | 113 | 3 | 9 | 251 | 3 | 240 | 5.68e-80 | 240 |
| Msa0662980 | MtrunA17_Chr8g0360041 | 50.667 | 225 | 109 | 1 | 24 | 248 | 28 | 250 | 4.11e-79 | 238 |
| Msa0662980 | MtrunA17_Chr7g0218741 | 51.316 | 228 | 108 | 2 | 24 | 250 | 26 | 251 | 1.22e-77 | 235 |
| Msa0662980 | MtrunA17_Chr4g0069711 | 50.222 | 225 | 109 | 2 | 27 | 250 | 61 | 283 | 4.19e-76 | 232 |
| Msa0662980 | MtrunA17_Chr7g0218761 | 52.195 | 205 | 96 | 1 | 45 | 249 | 7 | 209 | 7.68e-76 | 229 |
| Msa0662980 | MtrunA17_Chr1g0193631 | 48.927 | 233 | 115 | 3 | 20 | 249 | 23 | 254 | 2.63e-75 | 229 |
| Msa0662980 | MtrunA17_Chr7g0271001 | 46.154 | 247 | 125 | 4 | 8 | 247 | 4 | 249 | 4.11e-74 | 226 |
| Msa0662980 | MtrunA17_Chr6g0453341 | 48.606 | 251 | 114 | 3 | 6 | 251 | 12 | 252 | 1.57e-72 | 222 |
| Msa0662980 | MtrunA17_Chr8g0360721 | 51.724 | 203 | 96 | 1 | 45 | 247 | 16 | 216 | 2.19e-72 | 220 |
| Msa0662980 | MtrunA17_Chr5g0398271 | 41.532 | 248 | 143 | 2 | 3 | 249 | 13 | 259 | 1.69e-67 | 209 |
| Msa0662980 | MtrunA17_Chr4g0057091 | 43.983 | 241 | 133 | 2 | 8 | 247 | 17 | 256 | 3.47e-67 | 208 |
| Msa0662980 | MtrunA17_Chr1g0157061 | 44.037 | 218 | 117 | 3 | 34 | 249 | 24 | 238 | 6.48e-66 | 204 |
| Msa0662980 | MtrunA17_Chr2g0324181 | 85.149 | 101 | 15 | 0 | 151 | 251 | 17 | 117 | 4.45e-61 | 188 |
| Msa0662980 | MtrunA17_Chr4g0022841 | 51.304 | 115 | 56 | 0 | 134 | 248 | 2 | 116 | 1.91e-36 | 125 |
| Msa0662980 | MtrunA17_Chr2g0324221 | 31.020 | 245 | 141 | 9 | 18 | 249 | 26 | 255 | 3.78e-29 | 110 |
| Msa0662980 | MtrunA17_Chr4g0022851 | 43.443 | 122 | 65 | 2 | 3 | 124 | 8 | 125 | 1.65e-28 | 105 |
| Msa0662980 | MtrunA17_Chr4g0021641 | 32.353 | 238 | 135 | 9 | 24 | 249 | 38 | 261 | 1.04e-27 | 107 |
| Msa0662980 | MtrunA17_Chr2g0324201 | 30.544 | 239 | 138 | 10 | 24 | 249 | 30 | 253 | 3.71e-27 | 105 |
| Msa0662980 | MtrunA17_Chr4g0012801 | 31.439 | 264 | 145 | 12 | 3 | 247 | 13 | 259 | 9.96e-25 | 99.0 |
| Msa0662980 | MtrunA17_Chr8g0359971 | 53.947 | 76 | 34 | 1 | 173 | 247 | 2 | 77 | 9.62e-22 | 86.3 |
| Msa0662980 | MtrunA17_Chr8g0359981 | 39.683 | 126 | 49 | 5 | 68 | 190 | 1 | 102 | 1.51e-20 | 84.0 |
| Msa0662980 | MtrunA17_Chr7g0231221 | 29.004 | 231 | 136 | 11 | 32 | 247 | 53 | 270 | 7.54e-19 | 83.6 |
| Msa0662980 | MtrunA17_Chr5g0399811 | 28.194 | 227 | 146 | 9 | 30 | 249 | 32 | 248 | 1.34e-13 | 68.6 |
| Msa0662980 | MtrunA17_Chr4g0055601 | 26.336 | 262 | 155 | 10 | 5 | 249 | 8 | 248 | 1.80e-13 | 68.2 |
| Msa0662980 | MtrunA17_Chr4g0038741 | 26.106 | 226 | 132 | 10 | 38 | 247 | 32 | 238 | 3.15e-12 | 64.7 |
| Msa0662980 | MtrunA17_Chr1g0193571 | 49.091 | 55 | 28 | 0 | 195 | 249 | 3 | 57 | 2.12e-11 | 58.2 |
| Msa0662980 | MtrunA17_Chr4g0055561 | 25.200 | 250 | 168 | 9 | 5 | 247 | 8 | 245 | 3.34e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0662980 | AT4G38210.1 | 65.714 | 245 | 80 | 2 | 9 | 251 | 12 | 254 | 1.17e-119 | 342 |
| Msa0662980 | AT3G03220.1 | 57.080 | 226 | 96 | 1 | 26 | 251 | 39 | 263 | 9.95e-93 | 274 |
| Msa0662980 | AT3G55500.1 | 50.201 | 249 | 120 | 1 | 7 | 251 | 10 | 258 | 1.79e-88 | 263 |
| Msa0662980 | AT2G39700.1 | 47.809 | 251 | 131 | 0 | 1 | 251 | 5 | 255 | 4.95e-88 | 261 |
| Msa0662980 | AT5G02260.1 | 47.200 | 250 | 127 | 1 | 7 | 251 | 7 | 256 | 2.38e-87 | 260 |
| Msa0662980 | AT2G37640.1 | 49.004 | 251 | 120 | 1 | 9 | 251 | 10 | 260 | 3.97e-87 | 259 |
| Msa0662980 | AT5G05290.1 | 51.271 | 236 | 112 | 2 | 17 | 251 | 22 | 255 | 1.44e-85 | 255 |
| Msa0662980 | AT2G40610.1 | 50.201 | 249 | 121 | 3 | 4 | 251 | 7 | 253 | 3.19e-84 | 252 |
| Msa0662980 | AT1G69530.2 | 50.202 | 247 | 119 | 2 | 7 | 251 | 5 | 249 | 5.94e-84 | 251 |
| Msa0662980 | AT1G69530.1 | 50.202 | 247 | 119 | 2 | 7 | 251 | 5 | 249 | 5.94e-84 | 251 |
| Msa0662980 | AT5G56320.1 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 7.06e-84 | 251 |
| Msa0662980 | AT5G56320.2 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 7.06e-84 | 251 |
| Msa0662980 | AT2G03090.1 | 52.632 | 228 | 106 | 1 | 24 | 251 | 27 | 252 | 1.45e-83 | 250 |
| Msa0662980 | AT2G37640.2 | 53.333 | 210 | 98 | 0 | 42 | 251 | 26 | 235 | 4.23e-83 | 248 |
| Msa0662980 | AT5G56320.3 | 48.560 | 243 | 123 | 1 | 9 | 251 | 11 | 251 | 4.56e-83 | 251 |
| Msa0662980 | AT1G69530.3 | 50.413 | 242 | 116 | 2 | 7 | 246 | 5 | 244 | 4.76e-82 | 247 |
| Msa0662980 | AT2G28950.1 | 46.825 | 252 | 133 | 1 | 1 | 251 | 4 | 255 | 4.34e-81 | 244 |
| Msa0662980 | AT5G39280.1 | 51.327 | 226 | 106 | 2 | 24 | 249 | 51 | 272 | 8.73e-79 | 239 |
| Msa0662980 | AT5G39300.1 | 50.442 | 226 | 108 | 2 | 24 | 249 | 52 | 273 | 1.41e-77 | 236 |
| Msa0662980 | AT5G39270.1 | 50.885 | 226 | 107 | 2 | 24 | 249 | 55 | 276 | 7.87e-77 | 234 |
| Msa0662980 | AT5G39290.1 | 50.442 | 226 | 108 | 2 | 24 | 249 | 55 | 276 | 3.31e-76 | 232 |
| Msa0662980 | AT1G26770.1 | 48.207 | 251 | 127 | 2 | 1 | 251 | 1 | 248 | 6.99e-76 | 231 |
| Msa0662980 | AT1G26770.2 | 48.207 | 251 | 127 | 2 | 1 | 251 | 11 | 258 | 9.11e-76 | 231 |
| Msa0662980 | AT3G29030.1 | 46.768 | 263 | 118 | 5 | 1 | 251 | 1 | 253 | 1.65e-75 | 230 |
| Msa0662980 | AT1G62980.1 | 43.621 | 243 | 135 | 2 | 8 | 249 | 12 | 253 | 1.15e-73 | 225 |
| Msa0662980 | AT4G01630.1 | 47.321 | 224 | 114 | 3 | 27 | 247 | 28 | 250 | 1.28e-70 | 217 |
| Msa0662980 | AT5G39260.1 | 43.922 | 255 | 128 | 4 | 1 | 249 | 14 | 259 | 5.10e-70 | 216 |
| Msa0662980 | AT5G39310.1 | 48.661 | 224 | 110 | 3 | 27 | 249 | 90 | 309 | 1.57e-69 | 216 |
| Msa0662980 | AT1G20190.1 | 43.966 | 232 | 125 | 3 | 19 | 247 | 18 | 247 | 3.71e-69 | 213 |
| Msa0662980 | AT1G69530.4 | 44.130 | 247 | 104 | 3 | 7 | 251 | 5 | 219 | 2.24e-68 | 210 |
| Msa0662980 | AT1G69530.5 | 49.510 | 204 | 99 | 2 | 7 | 208 | 5 | 206 | 4.19e-67 | 208 |
| Msa0662980 | AT1G12560.1 | 43.443 | 244 | 133 | 4 | 7 | 247 | 15 | 256 | 5.64e-67 | 208 |
| Msa0662980 | AT3G15370.1 | 43.969 | 257 | 132 | 6 | 1 | 251 | 1 | 251 | 2.63e-65 | 204 |
| Msa0662980 | AT3G15370.2 | 40.418 | 287 | 129 | 8 | 1 | 251 | 1 | 281 | 1.18e-60 | 193 |
| Msa0662980 | AT1G20190.2 | 46.032 | 189 | 97 | 3 | 62 | 247 | 2 | 188 | 6.96e-59 | 185 |
| Msa0662980 | AT4G28250.1 | 32.365 | 241 | 132 | 11 | 24 | 249 | 33 | 257 | 3.24e-24 | 98.2 |
| Msa0662980 | AT2G20750.1 | 33.068 | 251 | 140 | 11 | 13 | 249 | 28 | 264 | 1.95e-23 | 96.3 |
| Msa0662980 | AT1G65680.1 | 29.839 | 248 | 143 | 9 | 17 | 247 | 35 | 268 | 2.46e-23 | 95.9 |
| Msa0662980 | AT4G28250.2 | 32.083 | 240 | 128 | 11 | 24 | 249 | 33 | 251 | 3.36e-22 | 92.4 |
| Msa0662980 | AT2G45110.1 | 30.172 | 232 | 135 | 11 | 30 | 247 | 36 | 254 | 9.84e-18 | 80.5 |
| Msa0662980 | AT1G65681.1 | 28.636 | 220 | 132 | 9 | 40 | 247 | 11 | 217 | 4.56e-17 | 77.8 |
| Msa0662980 | AT3G60570.1 | 25.483 | 259 | 159 | 10 | 10 | 249 | 11 | 254 | 1.66e-14 | 71.6 |
| Msa0662980 | AT2G20750.2 | 32.804 | 189 | 103 | 8 | 13 | 190 | 28 | 203 | 3.78e-14 | 69.7 |
| Msa0662980 | AT4G17030.1 | 25.969 | 258 | 151 | 11 | 8 | 247 | 9 | 244 | 2.98e-11 | 62.4 |
| Msa0662980 | AT3G60570.2 | 25.792 | 221 | 136 | 9 | 42 | 249 | 1 | 206 | 3.30e-11 | 61.6 |
Find 74 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCTGTCCTCCTAATTT+TGG | 0.175976 | 4_4:-994987 | Msa0662980:CDS |
| ATAAAATGAGTTGCTCTTCT+TGG | 0.221895 | 4_4:+993892 | None:intergenic |
| GCACCTCCAAACTGGCAATT+TGG | 0.255098 | 4_4:-994189 | Msa0662980:CDS |
| TAGAGATCCTTCTGTGTCTT+TGG | 0.259434 | 4_4:+995389 | None:intergenic |
| TTTGGTCTCTCAGTTGATTA+TGG | 0.289936 | 4_4:-994969 | Msa0662980:CDS |
| CTTATACAAATTATGATTCA+TGG | 0.302357 | 4_4:-994097 | Msa0662980:three_prime_UTR |
| GGATACCAATGGCAAGGAAT+TGG | 0.318465 | 4_4:-994308 | Msa0662980:CDS |
| GTTTGTCCAAATTGCCAGTT+TGG | 0.334344 | 4_4:+994183 | None:intergenic |
| TGTCATTCATATATGTAAAC+AGG | 0.335548 | 4_4:-994126 | Msa0662980:three_prime_UTR |
| TCAACTGAGAGACCAAAATT+AGG | 0.348503 | 4_4:+994975 | None:intergenic |
| GATACCAATGGCAAGGAATT+GGG | 0.350848 | 4_4:-994307 | Msa0662980:CDS |
| ACATCAGCCTTTGTCCTTTG+AGG | 0.354377 | 4_4:-994253 | Msa0662980:CDS |
| CTAAACGCTGTTATCTAAGA+TGG | 0.361741 | 4_4:-995511 | Msa0662980:exon |
| GACAACTCAAAATGTTCTCT+TGG | 0.372662 | 4_4:+994930 | None:intergenic |
| GCAGAAGGAGCTTGTGGTTA+TGG | 0.372980 | 4_4:-995167 | Msa0662980:intron |
| ACAGAAGGATCTCTAATTAC+AGG | 0.385780 | 4_4:-995381 | Msa0662980:intron |
| TTATTCCCGTTCAATATAGA+AGG | 0.389617 | 4_4:-994866 | Msa0662980:intron |
| CAATTTGGACAAACATTTGA+AGG | 0.398107 | 4_4:-994174 | Msa0662980:CDS |
| TTGCTTACAAAGATCAAGAA+TGG | 0.419745 | 4_4:-995434 | Msa0662980:CDS |
| CAAGGAATTGGGGAATGAAT+TGG | 0.429950 | 4_4:-994296 | Msa0662980:CDS |
| ATCTACCTTCTATATTGAAC+GGG | 0.431748 | 4_4:+994861 | None:intergenic |
| CAAGCATACACCACAAGATA+TGG | 0.436575 | 4_4:+995039 | None:intergenic |
| ATAACTAATGTGGGGTTAGA+TGG | 0.442300 | 4_4:-994372 | Msa0662980:CDS |
| ACAATGTAGCACCTCCAAAC+TGG | 0.451802 | 4_4:-994197 | Msa0662980:CDS |
| CTTTGTGTTGTGTATACCAT+TGG | 0.466580 | 4_4:+993964 | None:intergenic |
| GTTATATAAAATTGAGCCAA+TGG | 0.479724 | 4_4:-993980 | Msa0662980:three_prime_UTR |
| GAGTAATTAAATTTGCATGA+TGG | 0.482311 | 4_4:+993866 | None:intergenic |
| GAGGCAATGATGCTAGGAAA+AGG | 0.499306 | 4_4:+995602 | None:intergenic |
| ATTCCCCAATTCCTTGCCAT+TGG | 0.501334 | 4_4:+994303 | None:intergenic |
| AATTATGATTCATGGAATCA+TGG | 0.502165 | 4_4:-994089 | Msa0662980:three_prime_UTR |
| AAAGTGAAGGGATCTAGAAC+AGG | 0.502893 | 4_4:-994333 | Msa0662980:CDS |
| TGCATTTGCTGAAATTGCTA+AGG | 0.506123 | 4_4:-994901 | Msa0662980:CDS |
| TCTCAGTTGATTATGGTGGT+TGG | 0.506701 | 4_4:-994962 | Msa0662980:CDS |
| CAAGTTCTGATAACTAATGT+GGG | 0.508543 | 4_4:-994381 | Msa0662980:CDS |
| TCAAGTTCTGATAACTAATG+TGG | 0.509065 | 4_4:-994382 | Msa0662980:CDS |
| AGGACAAAGGCTGATGTTGA+AGG | 0.517191 | 4_4:+994259 | None:intergenic |
| GATCTTCACAAAGCTAGCTA+TGG | 0.521811 | 4_4:-995143 | Msa0662980:CDS |
| TAAACGCTGTTATCTAAGAT+GGG | 0.523848 | 4_4:-995510 | Msa0662980:exon |
| CATATCTTGTGGTGTATGCT+TGG | 0.525681 | 4_4:-995038 | Msa0662980:CDS |
| GTCTTTGGCATAAGTTGCAG+TGG | 0.528308 | 4_4:+995404 | None:intergenic |
| TATCTACCTTCTATATTGAA+CGG | 0.536318 | 4_4:+994860 | None:intergenic |
| TGTCCAAATTGCCAGTTTGG+AGG | 0.539604 | 4_4:+994186 | None:intergenic |
| GTGAAGTGTGAAAGAAGTGG+AGG | 0.540916 | 4_4:-994441 | Msa0662980:CDS |
| GCATTTGCTGAAATTGCTAA+GGG | 0.541056 | 4_4:-994900 | Msa0662980:CDS |
| GAGGTATCCTGATATGTAGT+AGG | 0.545646 | 4_4:-995639 | None:intergenic |
| TTTGAGGTAACTAGCAGCAC+TGG | 0.546125 | 4_4:-994237 | Msa0662980:CDS |
| ATATGTGAGGCAATGATGCT+AGG | 0.552240 | 4_4:+995596 | None:intergenic |
| AGGCAATGATGCTAGGAAAA+GGG | 0.552818 | 4_4:+995603 | None:intergenic |
| TTCAACAGAGGGAGTACATG+TGG | 0.560849 | 4_4:-995089 | Msa0662980:CDS |
| AGCTATGGAAAACATAGTGT+TGG | 0.560850 | 4_4:-995128 | Msa0662980:CDS |
| GGGTTGAAATTCACAATGAG+TGG | 0.572612 | 4_4:-994420 | Msa0662980:CDS |
| GGTCTCTCAGTTGATTATGG+TGG | 0.580447 | 4_4:-994966 | Msa0662980:CDS |
| ATACCAATGGCAAGGAATTG+GGG | 0.587228 | 4_4:-994306 | Msa0662980:CDS |
| TCAACAGAGGGAGTACATGT+GGG | 0.614475 | 4_4:-995088 | Msa0662980:CDS |
| TAAAATCAAGTAGAGAAGTG+TGG | 0.616273 | 4_4:+995476 | None:intergenic |
| TGAAGTGTGAAAGAAGTGGA+GGG | 0.622177 | 4_4:-994440 | Msa0662980:CDS |
| TGAAGGGATCTAGAACAGGA+TGG | 0.625878 | 4_4:-994329 | Msa0662980:CDS |
| ACTGAGAGACCAAAATTAGG+AGG | 0.626412 | 4_4:+994978 | None:intergenic |
| CAGGATGGATACCAATGGCA+AGG | 0.630186 | 4_4:-994314 | Msa0662980:CDS |
| CTAGTTACCTCAAAGGACAA+AGG | 0.637645 | 4_4:+994246 | None:intergenic |
| GTCATTCATATATGTAAACA+GGG | 0.643903 | 4_4:-994125 | Msa0662980:three_prime_UTR |
| GTGGCAGTAACAATAACAGA+AGG | 0.647537 | 4_4:+995011 | None:intergenic |
| TAGAACAGGATGGATACCAA+TGG | 0.662207 | 4_4:-994319 | Msa0662980:CDS |
| CAACAGAGGGAGTACATGTG+GGG | 0.668401 | 4_4:-995087 | Msa0662980:CDS |
| ACTTATGCCAAAGACACAGA+AGG | 0.668875 | 4_4:-995396 | Msa0662980:CDS |
| AGTGCTGCTAGTTACCTCAA+AGG | 0.674064 | 4_4:+994239 | None:intergenic |
| CATTTGCTGAAATTGCTAAG+GGG | 0.677827 | 4_4:-994899 | Msa0662980:CDS |
| TGATCAAAGAATTGTGCAAG+TGG | 0.686079 | 4_4:-994033 | Msa0662980:three_prime_UTR |
| AAGTTCTGATAACTAATGTG+GGG | 0.688292 | 4_4:-994380 | Msa0662980:CDS |
| ATTAGGAGGACAGAAATCAG+TGG | 0.688419 | 4_4:+994992 | None:intergenic |
| TGTGATGCAGAAGGAGCTTG+TGG | 0.691735 | 4_4:-995173 | Msa0662980:intron |
| AGAGTGAAGTGTGAAAGAAG+TGG | 0.695816 | 4_4:-994444 | Msa0662980:CDS |
| TCAAAGAATTGTGCAAGTGG+TGG | 0.708131 | 4_4:-994030 | Msa0662980:three_prime_UTR |
| GATGTGTTGACCATATCTTG+TGG | 0.783191 | 4_4:-995049 | Msa0662980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATGAAATAATTTACTTTT+AGG | + | chr4_4:993948-993967 | None:intergenic | 10.0% |
| !!! | ATTTTTTTATAAGTGTTTTT+GGG | - | chr4_4:994667-994686 | Msa0662980:intron | 10.0% |
| !!! | TCATGTACATTTTTTTTTTT+TGG | + | chr4_4:994298-994317 | None:intergenic | 15.0% |
| !!! | GATTTTTTTATAAGTGTTTT+TGG | - | chr4_4:994666-994685 | Msa0662980:intron | 15.0% |
| !! | AACTTAATTAATCTCTTATT+TGG | - | chr4_4:994706-994725 | Msa0662980:intron | 15.0% |
| !!! | TGTACATTTTTTTTTTTTGG+TGG | + | chr4_4:994295-994314 | None:intergenic | 20.0% |
| !! | GTTTATATAAAAACAGTTCA+AGG | + | chr4_4:994791-994810 | None:intergenic | 20.0% |
| !! | AAATGAAACTAAAAAACACA+AGG | + | chr4_4:994858-994877 | None:intergenic | 20.0% |
| !!! | TGTGTTTTTTAGTTTCATTT+TGG | - | chr4_4:994858-994877 | Msa0662980:intron | 20.0% |
| !! | ATAACATAGCTAATAAAAAG+AGG | + | chr4_4:994940-994959 | None:intergenic | 20.0% |
| !!! | ATTTGAAGGAAAACAATTTT+AGG | - | chr4_4:995343-995362 | Msa0662980:intron | 20.0% |
| !! | CTTATACAAATTATGATTCA+TGG | - | chr4_4:995406-995425 | Msa0662980:CDS | 20.0% |
| !!! | ACATTTTTTTTTTTTGGTGG+TGG | + | chr4_4:994292-994311 | None:intergenic | 25.0% |
| ! | TATCTACCTTCTATATTGAA+CGG | + | chr4_4:994646-994665 | None:intergenic | 25.0% |
| ! | AAAAAGAGGCAAAAAAGATT+AGG | + | chr4_4:994926-994945 | None:intergenic | 25.0% |
| ! | GCACAAACAAAACAAAAATT+TGG | + | chr4_4:995028-995047 | None:intergenic | 25.0% |
| ! | TGTCATTCATATATGTAAAC+AGG | - | chr4_4:995377-995396 | Msa0662980:intron | 25.0% |
| ! | GTCATTCATATATGTAAACA+GGG | - | chr4_4:995378-995397 | Msa0662980:intron | 25.0% |
| ! | AATTATGATTCATGGAATCA+TGG | - | chr4_4:995414-995433 | Msa0662980:CDS | 25.0% |
| ! | GTTATATAAAATTGAGCCAA+TGG | - | chr4_4:995523-995542 | Msa0662980:five_prime_UTR | 25.0% |
| ! | TTGTGTTTTTCTCATATGTG+AGG | + | chr4_4:993923-993942 | None:intergenic | 30.0% |
| ! | TAAACGCTGTTATCTAAGAT+GGG | - | chr4_4:993993-994012 | Msa0662980:three_prime_UTR | 30.0% |
| TAAAATCAAGTAGAGAAGTG+TGG | + | chr4_4:994030-994049 | None:intergenic | 30.0% | |
| TTGCTTACAAAGATCAAGAA+TGG | - | chr4_4:994069-994088 | Msa0662980:three_prime_UTR | 30.0% | |
| CATAATATATGCAAGATCCA+GGG | - | chr4_4:994254-994273 | Msa0662980:CDS | 30.0% | |
| !!! | TTTTTTTTTTTGGTGGTGGT+CGG | + | chr4_4:994288-994307 | None:intergenic | 30.0% |
| TTATTCCCGTTCAATATAGA+AGG | - | chr4_4:994637-994656 | Msa0662980:intron | 30.0% | |
| ATCTACCTTCTATATTGAAC+GGG | + | chr4_4:994645-994664 | None:intergenic | 30.0% | |
| !!! | AAGTGTTTTTGGGATGTTTT+TGG | - | chr4_4:994677-994696 | Msa0662980:intron | 30.0% |
| ! | GTTTTGCTCAATTACACATT+TGG | - | chr4_4:994811-994830 | Msa0662980:intron | 30.0% |
| ! | TCAAGTTCTGATAACTAATG+TGG | - | chr4_4:995121-995140 | Msa0662980:CDS | 30.0% |
| ! | CAAGTTCTGATAACTAATGT+GGG | - | chr4_4:995122-995141 | Msa0662980:CDS | 30.0% |
| ! | AAGTTCTGATAACTAATGTG+GGG | - | chr4_4:995123-995142 | Msa0662980:CDS | 30.0% |
| CAATTTGGACAAACATTTGA+AGG | - | chr4_4:995329-995348 | Msa0662980:intron | 30.0% | |
| ! | AACAATTTTAGGCTTCAACA+TGG | - | chr4_4:995354-995373 | Msa0662980:intron | 30.0% |
| ! | ATGAGCAAATATACCGTTTT+TGG | + | chr4_4:995444-995463 | None:intergenic | 30.0% |
| ATAAAATGAGTTGCTCTTCT+TGG | + | chr4_4:995614-995633 | None:intergenic | 30.0% | |
| CTAAACGCTGTTATCTAAGA+TGG | - | chr4_4:993992-994011 | Msa0662980:three_prime_UTR | 35.0% | |
| ACAGAAGGATCTCTAATTAC+AGG | - | chr4_4:994122-994141 | Msa0662980:three_prime_UTR | 35.0% | |
| ! | ATGTTGCTGTTATGAACCAA+AGG | + | chr4_4:994164-994183 | None:intergenic | 35.0% |
| GCATAATATATGCAAGATCC+AGG | - | chr4_4:994253-994272 | Msa0662980:CDS | 35.0% | |
| !!! | TTTTTTTTTTGGTGGTGGTC+GGG | + | chr4_4:994287-994306 | None:intergenic | 35.0% |
| AGCTATGGAAAACATAGTGT+TGG | - | chr4_4:994375-994394 | Msa0662980:CDS | 35.0% | |
| ! | AGCACAATTTTGTTCAACAG+AGG | - | chr4_4:994402-994421 | Msa0662980:CDS | 35.0% |
| ! | GCACAATTTTGTTCAACAGA+GGG | - | chr4_4:994403-994422 | Msa0662980:CDS | 35.0% |
| ! | GATTTCTGTCCTCCTAATTT+TGG | - | chr4_4:994516-994535 | Msa0662980:intron | 35.0% |
| TCAACTGAGAGACCAAAATT+AGG | + | chr4_4:994531-994550 | None:intergenic | 35.0% | |
| TTTGGTCTCTCAGTTGATTA+TGG | - | chr4_4:994534-994553 | Msa0662980:intron | 35.0% | |
| GACAACTCAAAATGTTCTCT+TGG | + | chr4_4:994576-994595 | None:intergenic | 35.0% | |
| !!! | AGAACATTTTGAGTTGTCAC+TGG | - | chr4_4:994578-994597 | Msa0662980:intron | 35.0% |
| ! | TGCATTTGCTGAAATTGCTA+AGG | - | chr4_4:994602-994621 | Msa0662980:intron | 35.0% |
| ! | GCATTTGCTGAAATTGCTAA+GGG | - | chr4_4:994603-994622 | Msa0662980:intron | 35.0% |
| ! | CATTTGCTGAAATTGCTAAG+GGG | - | chr4_4:994604-994623 | Msa0662980:intron | 35.0% |
| ATAACTAATGTGGGGTTAGA+TGG | - | chr4_4:995131-995150 | Msa0662980:CDS | 35.0% | |
| !! | AGTTTTTGCTGTGAAAGTGA+AGG | - | chr4_4:995157-995176 | Msa0662980:CDS | 35.0% |
| !! | GTTTTTGCTGTGAAAGTGAA+GGG | - | chr4_4:995158-995177 | Msa0662980:CDS | 35.0% |
| GAATCATGGTAGACCAAAAA+CGG | - | chr4_4:995428-995447 | Msa0662980:CDS | 35.0% | |
| TGATCAAAGAATTGTGCAAG+TGG | - | chr4_4:995470-995489 | Msa0662980:CDS | 35.0% | |
| CTTTGTGTTGTGTATACCAT+TGG | + | chr4_4:995542-995561 | None:intergenic | 35.0% | |
| !!! | ATGACTGCTTTTTTGGTCAA+TGG | + | chr4_4:995575-995594 | None:intergenic | 35.0% |
| !!! | TTCGTCAATGACTGCTTTTT+TGG | + | chr4_4:995582-995601 | None:intergenic | 35.0% |
| AGGCAATGATGCTAGGAAAA+GGG | + | chr4_4:993903-993922 | None:intergenic | 40.0% | |
| ATATGTGAGGCAATGATGCT+AGG | + | chr4_4:993910-993929 | None:intergenic | 40.0% | |
| ACTTATGCCAAAGACACAGA+AGG | - | chr4_4:994107-994126 | Msa0662980:three_prime_UTR | 40.0% | |
| TAGAGATCCTTCTGTGTCTT+TGG | + | chr4_4:994117-994136 | None:intergenic | 40.0% | |
| ! | TACTTTTCTTGCTCCTCCTT+TGG | - | chr4_4:994145-994164 | Msa0662980:exon | 40.0% |
| ! | TTGCTGTTATGAACCAAAGG+AGG | + | chr4_4:994161-994180 | None:intergenic | 40.0% |
| !!! | TTTTTTTTTGGTGGTGGTCG+GGG | + | chr4_4:994286-994305 | None:intergenic | 40.0% |
| GATCTTCACAAAGCTAGCTA+TGG | - | chr4_4:994360-994379 | Msa0662980:CDS | 40.0% | |
| ! | GATGTGTTGACCATATCTTG+TGG | - | chr4_4:994454-994473 | Msa0662980:intron | 40.0% |
| CAAGCATACACCACAAGATA+TGG | + | chr4_4:994467-994486 | None:intergenic | 40.0% | |
| CATATCTTGTGGTGTATGCT+TGG | - | chr4_4:994465-994484 | Msa0662980:intron | 40.0% | |
| GTGGCAGTAACAATAACAGA+AGG | + | chr4_4:994495-994514 | None:intergenic | 40.0% | |
| ATTAGGAGGACAGAAATCAG+TGG | + | chr4_4:994514-994533 | None:intergenic | 40.0% | |
| ACTGAGAGACCAAAATTAGG+AGG | + | chr4_4:994528-994547 | None:intergenic | 40.0% | |
| TCTCAGTTGATTATGGTGGT+TGG | - | chr4_4:994541-994560 | Msa0662980:intron | 40.0% | |
| AGAGTGAAGTGTGAAAGAAG+TGG | - | chr4_4:995059-995078 | Msa0662980:CDS | 40.0% | |
| TGAAGTGTGAAAGAAGTGGA+GGG | - | chr4_4:995063-995082 | Msa0662980:CDS | 40.0% | |
| GGGTTGAAATTCACAATGAG+TGG | - | chr4_4:995083-995102 | Msa0662980:CDS | 40.0% | |
| ! | AAAGTGAAGGGATCTAGAAC+AGG | - | chr4_4:995170-995189 | Msa0662980:intron | 40.0% |
| TAGAACAGGATGGATACCAA+TGG | - | chr4_4:995184-995203 | Msa0662980:intron | 40.0% | |
| GATACCAATGGCAAGGAATT+GGG | - | chr4_4:995196-995215 | Msa0662980:intron | 40.0% | |
| ATACCAATGGCAAGGAATTG+GGG | - | chr4_4:995197-995216 | Msa0662980:intron | 40.0% | |
| CAAGGAATTGGGGAATGAAT+TGG | - | chr4_4:995207-995226 | Msa0662980:intron | 40.0% | |
| CTAGTTACCTCAAAGGACAA+AGG | + | chr4_4:995260-995279 | None:intergenic | 40.0% | |
| GTTTGTCCAAATTGCCAGTT+TGG | + | chr4_4:995323-995342 | None:intergenic | 40.0% | |
| TCAAAGAATTGTGCAAGTGG+TGG | - | chr4_4:995473-995492 | Msa0662980:CDS | 40.0% | |
| GAGGCAATGATGCTAGGAAA+AGG | + | chr4_4:993904-993923 | None:intergenic | 45.0% | |
| GTCTTTGGCATAAGTTGCAG+TGG | + | chr4_4:994102-994121 | None:intergenic | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+TGG | - | chr4_4:994225-994244 | Msa0662980:CDS | 45.0% |
| TTGTGTGCATGTGATGCAGA+AGG | - | chr4_4:994321-994340 | Msa0662980:CDS | 45.0% | |
| ! | TTCAACAGAGGGAGTACATG+TGG | - | chr4_4:994414-994433 | Msa0662980:CDS | 45.0% |
| ! | TCAACAGAGGGAGTACATGT+GGG | - | chr4_4:994415-994434 | Msa0662980:CDS | 45.0% |
| GGTCTCTCAGTTGATTATGG+TGG | - | chr4_4:994537-994556 | Msa0662980:intron | 45.0% | |
| GTGAAGTGTGAAAGAAGTGG+AGG | - | chr4_4:995062-995081 | Msa0662980:CDS | 45.0% | |
| ! | TGAAGGGATCTAGAACAGGA+TGG | - | chr4_4:995174-995193 | Msa0662980:intron | 45.0% |
| GGATACCAATGGCAAGGAAT+TGG | - | chr4_4:995195-995214 | Msa0662980:intron | 45.0% | |
| ATTCCCCAATTCCTTGCCAT+TGG | + | chr4_4:995203-995222 | None:intergenic | 45.0% | |
| AGGACAAAGGCTGATGTTGA+AGG | + | chr4_4:995247-995266 | None:intergenic | 45.0% | |
| ACATCAGCCTTTGTCCTTTG+AGG | - | chr4_4:995250-995269 | Msa0662980:intron | 45.0% | |
| ! | AGTGCTGCTAGTTACCTCAA+AGG | + | chr4_4:995267-995286 | None:intergenic | 45.0% |
| ! | TTTGAGGTAACTAGCAGCAC+TGG | - | chr4_4:995266-995285 | Msa0662980:intron | 45.0% |
| ACAATGTAGCACCTCCAAAC+TGG | - | chr4_4:995306-995325 | Msa0662980:intron | 45.0% | |
| TGTCCAAATTGCCAGTTTGG+AGG | + | chr4_4:995320-995339 | None:intergenic | 45.0% | |
| TGTGATGCAGAAGGAGCTTG+TGG | - | chr4_4:994330-994349 | Msa0662980:CDS | 50.0% | |
| GCAGAAGGAGCTTGTGGTTA+TGG | - | chr4_4:994336-994355 | Msa0662980:CDS | 50.0% | |
| ! | CAACAGAGGGAGTACATGTG+GGG | - | chr4_4:994416-994435 | Msa0662980:CDS | 50.0% |
| CAGGATGGATACCAATGGCA+AGG | - | chr4_4:995189-995208 | Msa0662980:intron | 50.0% | |
| GCACCTCCAAACTGGCAATT+TGG | - | chr4_4:995314-995333 | Msa0662980:intron | 50.0% | |
| CCAACTGAGCTAAGCTCACG+AGG | + | chr4_4:994223-994242 | None:intergenic | 55.0% | |
| CCTCGTGAGCTTAGCTCAGT+TGG | - | chr4_4:994220-994239 | Msa0662980:CDS | 55.0% | |
| GGTGGTCGGGGTTTGAACCC+TGG | + | chr4_4:994274-994293 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 993874 | 995651 | 993874 | ID=Msa0662980;Name=Msa0662980 |
| chr4_4 | mRNA | 993874 | 995651 | 993874 | ID=Msa0662980-mRNA-1;Parent=Msa0662980;Name=Msa0662980-mRNA-1;_AED=0.05;_eAED=0.05;_QI=137|1|1|1|1|1|3|287|251 |
| chr4_4 | exon | 993874 | 994464 | 993874 | ID=Msa0662980-mRNA-1:exon:391;Parent=Msa0662980-mRNA-1 |
| chr4_4 | exon | 994867 | 995185 | 994867 | ID=Msa0662980-mRNA-1:exon:390;Parent=Msa0662980-mRNA-1 |
| chr4_4 | exon | 995382 | 995651 | 995382 | ID=Msa0662980-mRNA-1:exon:389;Parent=Msa0662980-mRNA-1 |
| chr4_4 | five_prime_UTR | 995515 | 995651 | 995515 | ID=Msa0662980-mRNA-1:five_prime_utr;Parent=Msa0662980-mRNA-1 |
| chr4_4 | CDS | 995382 | 995514 | 995382 | ID=Msa0662980-mRNA-1:cds;Parent=Msa0662980-mRNA-1 |
| chr4_4 | CDS | 994867 | 995185 | 994867 | ID=Msa0662980-mRNA-1:cds;Parent=Msa0662980-mRNA-1 |
| chr4_4 | CDS | 994161 | 994464 | 994161 | ID=Msa0662980-mRNA-1:cds;Parent=Msa0662980-mRNA-1 |
| chr4_4 | three_prime_UTR | 993874 | 994160 | 993874 | ID=Msa0662980-mRNA-1:three_prime_utr;Parent=Msa0662980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
