Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568850 | KYP58465.1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 5 | 140 | 1.76e-92 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568850 | sp|P68428|H32_WHEAT | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.15e-95 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568850 | A0A1D1ZGP1 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 1.97e-92 | 272 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0115550 | Msa0568850 | 0.814412 | 1.560929e-51 | -8.615850e-47 |
| Msa0233070 | Msa0568850 | 0.836260 | 1.038393e-56 | -8.615850e-47 |
| Msa0568830 | Msa0568850 | 0.899389 | 2.079700e-77 | -8.615850e-47 |
| Msa0568850 | Msa0568900 | 0.904219 | 1.542294e-79 | -8.615850e-47 |
| Msa0568850 | Msa0645340 | 0.826117 | 3.234273e-54 | -8.615850e-47 |
| Msa0568850 | Msa0900760 | 0.806341 | 8.622104e-50 | -8.615850e-47 |
| Msa0568850 | Msa1296330 | 0.805952 | 1.040934e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568850 | MtrunA17_Chr5g0410931 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr5g0410951 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr8g0383851 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr8g0383781 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr8g0390361 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr2g0295191 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr7g0237661 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr4g0033041 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr7g0218971 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr4g0046301 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr8g0364801 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 3.79e-96 | 272 |
| Msa0568850 | MtrunA17_Chr4g0054161 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568850 | MtrunA17_Chr8g0363741 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568850 | MtrunA17_Chr1g0156021 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 2.60e-93 | 265 |
| Msa0568850 | MtrunA17_Chr4g0054151 | 97.059 | 136 | 4 | 0 | 1 | 136 | 4 | 139 | 2.61e-93 | 265 |
| Msa0568850 | MtrunA17_Chr8g0388121 | 96.324 | 136 | 5 | 0 | 1 | 136 | 1 | 136 | 1.09e-92 | 264 |
| Msa0568850 | MtrunA17_Chr2g0319141 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0568850 | MtrunA17_Chr2g0319151 | 89.706 | 136 | 14 | 0 | 1 | 136 | 1 | 136 | 1.10e-86 | 248 |
| Msa0568850 | MtrunA17_Chr2g0319131 | 85.616 | 146 | 11 | 1 | 1 | 136 | 1 | 146 | 1.38e-82 | 238 |
| Msa0568850 | MtrunA17_Chr2g0319121 | 84.559 | 136 | 19 | 2 | 1 | 135 | 1 | 135 | 5.82e-75 | 219 |
| Msa0568850 | MtrunA17_Chr7g0218961 | 86.607 | 112 | 15 | 0 | 25 | 136 | 2 | 113 | 8.01e-66 | 195 |
| Msa0568850 | MtrunA17_Chr7g0222991 | 67.164 | 134 | 43 | 1 | 1 | 134 | 10 | 142 | 5.47e-56 | 171 |
| Msa0568850 | MtrunA17_Chr5g0407311 | 67.241 | 116 | 35 | 2 | 1 | 116 | 1 | 113 | 1.29e-45 | 144 |
| Msa0568850 | MtrunA17_Chr7g0223001 | 58.647 | 133 | 41 | 3 | 3 | 134 | 27 | 146 | 5.46e-43 | 139 |
| Msa0568850 | MtrunA17_Chr7g0240791 | 100.000 | 66 | 0 | 0 | 1 | 66 | 1 | 66 | 9.61e-41 | 133 |
| Msa0568850 | MtrunA17_Chr8g0347231 | 55.102 | 98 | 43 | 1 | 37 | 133 | 28 | 125 | 3.80e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0568850 | AT1G09200.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568850 | AT5G65360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568850 | AT5G10390.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568850 | AT5G10400.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568850 | AT3G27360.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.62e-95 | 271 |
| Msa0568850 | AT4G40030.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40030.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40030.4 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT5G10980.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40040.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40040.3 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40040.1 | 97.059 | 136 | 4 | 0 | 1 | 136 | 1 | 136 | 3.59e-93 | 265 |
| Msa0568850 | AT4G40030.2 | 97.059 | 136 | 4 | 0 | 1 | 136 | 29 | 164 | 7.05e-93 | 266 |
| Msa0568850 | AT5G65350.1 | 94.853 | 136 | 7 | 0 | 1 | 136 | 1 | 136 | 5.30e-91 | 260 |
| Msa0568850 | AT1G75600.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.25e-89 | 256 |
| Msa0568850 | AT1G13370.1 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 5.55e-89 | 254 |
| Msa0568850 | AT1G19890.1 | 89.781 | 137 | 13 | 1 | 1 | 136 | 1 | 137 | 1.07e-83 | 241 |
| Msa0568850 | AT5G12910.1 | 71.111 | 135 | 34 | 2 | 1 | 135 | 1 | 130 | 9.90e-67 | 198 |
| Msa0568850 | AT1G01370.2 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568850 | AT1G01370.1 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568850 | AT1G01370.4 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
| Msa0568850 | AT1G01370.3 | 53.982 | 113 | 49 | 2 | 24 | 134 | 63 | 174 | 2.97e-31 | 110 |
Find 37 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTGTGGCAAGCTGCTTC+CGG | 0.216226 | 4_1:-70500831 | None:intergenic |
| CTTTGTGGCAAGCTGCTTCC+GGG | 0.250177 | 4_1:-70500830 | None:intergenic |
| CAGTGCTGTTTCTGCTCTTC+AGG | 0.258870 | 4_1:+70501041 | Msa0568850:CDS |
| TCTTGCAAGTTGAATGTCCT+TGG | 0.320782 | 4_1:-70501145 | None:intergenic |
| TCTCTCAAAGCAACTGTTCC+AGG | 0.333816 | 4_1:-70500910 | None:intergenic |
| CTTGCAAGTTGAATGTCCTT+GGG | 0.355623 | 4_1:-70501144 | None:intergenic |
| GGAAGCTGCTGAAGCTTACT+TGG | 0.372633 | 4_1:+70501062 | Msa0568850:CDS |
| CAGTGCTCTTCTGATACTTC+CGG | 0.409692 | 4_1:-70500936 | None:intergenic |
| TGATTTCCGAGCGGCCTTTG+TGG | 0.410237 | 4_1:-70500845 | None:intergenic |
| GAGCAGAAACAGCACTGCTC+TGG | 0.422418 | 4_1:-70501035 | None:intergenic |
| TTCTTCACTCCTCCGGTCGC+CGG | 0.442119 | 4_1:-70500871 | None:intergenic |
| GACGTTGGAATGGAAGTTTA+CGG | 0.458530 | 4_1:-70500969 | None:intergenic |
| CGGAAATCAGCTCCGGCGAC+CGG | 0.461897 | 4_1:+70500859 | Msa0568850:CDS |
| GGACTTGCGAGCTGTTTGCT+TGG | 0.471270 | 4_1:-70500791 | None:intergenic |
| CCACCGGCGGCAAGGCTCCC+CGG | 0.480154 | 4_1:+70500812 | Msa0568850:CDS |
| GCTGCTGAAGCTTACTTGGT+TGG | 0.483475 | 4_1:+70501066 | Msa0568850:CDS |
| GGCCGCTCGGAAATCAGCTC+CGG | 0.498921 | 4_1:+70500852 | Msa0568850:CDS |
| GTGTGGCTTCTTCACTCCTC+CGG | 0.499925 | 4_1:-70500878 | None:intergenic |
| CTTCCGGGGAGCCTTGCCGC+CGG | 0.507682 | 4_1:-70500815 | None:intergenic |
| AGCTTGCCACAAAGGCCGCT+CGG | 0.528264 | 4_1:+70500839 | Msa0568850:CDS |
| TCGCAAGTCCACCGGCGGCA+AGG | 0.530180 | 4_1:+70500804 | Msa0568850:CDS |
| CCGGGGAGCCTTGCCGCCGG+TGG | 0.548377 | 4_1:-70500812 | None:intergenic |
| CCGGAAGCAGCTTGCCACAA+AGG | 0.551090 | 4_1:+70500831 | Msa0568850:CDS |
| CAGTTGCTTTGAGAGAGATC+CGG | 0.552662 | 4_1:+70500917 | Msa0568850:CDS |
| TTTGTGGCAAGCTGCTTCCG+GGG | 0.553488 | 4_1:-70500829 | None:intergenic |
| CAAACAGCTCGCAAGTCCAC+CGG | 0.573307 | 4_1:+70500796 | Msa0568850:CDS |
| AAGAAGAATCAGAGGCGAGA+GGG | 0.597241 | 4_1:+70501164 | Msa0568850:CDS |
| TCACGAACAAGACGTTGGAA+TGG | 0.598115 | 4_1:-70500979 | None:intergenic |
| AAATCAGCTCCGGCGACCGG+AGG | 0.599225 | 4_1:+70500862 | Msa0568850:CDS |
| CGATTTCACGAACAAGACGT+TGG | 0.613448 | 4_1:-70500984 | None:intergenic |
| GAGAGTTACAATTATGCCCA+AGG | 0.621244 | 4_1:+70501128 | Msa0568850:CDS |
| CAAGAAGAATCAGAGGCGAG+AGG | 0.648850 | 4_1:+70501163 | Msa0568850:CDS |
| ACAGCTCGCAAGTCCACCGG+CGG | 0.655092 | 4_1:+70500799 | Msa0568850:CDS |
| CAACTTGCAAGAAGAATCAG+AGG | 0.655500 | 4_1:+70501156 | Msa0568850:CDS |
| AAGCCACACAGATTCAGACC+TGG | 0.672238 | 4_1:+70500892 | Msa0568850:CDS |
| GTTCCAGGTCTGAATCTGTG+TGG | 0.688220 | 4_1:-70500895 | None:intergenic |
| CGCCGGAGCTGATTTCCGAG+CGG | 0.728257 | 4_1:-70500854 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GACGTTGGAATGGAAGTTTA+CGG | - | chr4_1:70500972-70500991 | None:intergenic | 40.0% | |
| GAGAGTTACAATTATGCCCA+AGG | + | chr4_1:70501128-70501147 | Msa0568850:CDS | 40.0% | |
| ! | CTTGCAAGTTGAATGTCCTT+GGG | - | chr4_1:70501147-70501166 | None:intergenic | 40.0% |
| ! | TCTTGCAAGTTGAATGTCCT+TGG | - | chr4_1:70501148-70501167 | None:intergenic | 40.0% |
| CAACTTGCAAGAAGAATCAG+AGG | + | chr4_1:70501156-70501175 | Msa0568850:CDS | 40.0% | |
| TCTCTCAAAGCAACTGTTCC+AGG | - | chr4_1:70500913-70500932 | None:intergenic | 45.0% | |
| !! | CAGTTGCTTTGAGAGAGATC+CGG | + | chr4_1:70500917-70500936 | Msa0568850:CDS | 45.0% |
| CAGTGCTCTTCTGATACTTC+CGG | - | chr4_1:70500939-70500958 | None:intergenic | 45.0% | |
| TCACGAACAAGACGTTGGAA+TGG | - | chr4_1:70500982-70501001 | None:intergenic | 45.0% | |
| CGATTTCACGAACAAGACGT+TGG | - | chr4_1:70500987-70501006 | None:intergenic | 45.0% | |
| AAGAAGAATCAGAGGCGAGA+GGG | + | chr4_1:70501164-70501183 | Msa0568850:CDS | 45.0% | |
| AAGCCACACAGATTCAGACC+TGG | + | chr4_1:70500892-70500911 | Msa0568850:CDS | 50.0% | |
| GTTCCAGGTCTGAATCTGTG+TGG | - | chr4_1:70500898-70500917 | None:intergenic | 50.0% | |
| !! | CAGTGCTGTTTCTGCTCTTC+AGG | + | chr4_1:70501041-70501060 | Msa0568850:CDS | 50.0% |
| GGAAGCTGCTGAAGCTTACT+TGG | + | chr4_1:70501062-70501081 | Msa0568850:CDS | 50.0% | |
| GCTGCTGAAGCTTACTTGGT+TGG | + | chr4_1:70501066-70501085 | Msa0568850:CDS | 50.0% | |
| CAAGAAGAATCAGAGGCGAG+AGG | + | chr4_1:70501163-70501182 | Msa0568850:CDS | 50.0% | |
| GGACTTGCGAGCTGTTTGCT+TGG | - | chr4_1:70500794-70500813 | None:intergenic | 55.0% | |
| CAAACAGCTCGCAAGTCCAC+CGG | + | chr4_1:70500796-70500815 | Msa0568850:CDS | 55.0% | |
| TTTGTGGCAAGCTGCTTCCG+GGG | - | chr4_1:70500832-70500851 | None:intergenic | 55.0% | |
| CTTTGTGGCAAGCTGCTTCC+GGG | - | chr4_1:70500833-70500852 | None:intergenic | 55.0% | |
| CCTTTGTGGCAAGCTGCTTC+CGG | - | chr4_1:70500834-70500853 | None:intergenic | 55.0% | |
| TGATTTCCGAGCGGCCTTTG+TGG | - | chr4_1:70500848-70500867 | None:intergenic | 55.0% | |
| GTGTGGCTTCTTCACTCCTC+CGG | - | chr4_1:70500881-70500900 | None:intergenic | 55.0% | |
| GAGCAGAAACAGCACTGCTC+TGG | - | chr4_1:70501038-70501057 | None:intergenic | 55.0% | |
| CCGGAAGCAGCTTGCCACAA+AGG | + | chr4_1:70500831-70500850 | Msa0568850:CDS | 60.0% | |
| AGCTTGCCACAAAGGCCGCT+CGG | + | chr4_1:70500839-70500858 | Msa0568850:CDS | 60.0% | |
| TTCTTCACTCCTCCGGTCGC+CGG | - | chr4_1:70500874-70500893 | None:intergenic | 60.0% | |
| ACAGCTCGCAAGTCCACCGG+CGG | + | chr4_1:70500799-70500818 | Msa0568850:CDS | 65.0% | |
| GGCCGCTCGGAAATCAGCTC+CGG | + | chr4_1:70500852-70500871 | Msa0568850:CDS | 65.0% | |
| ! | CGCCGGAGCTGATTTCCGAG+CGG | - | chr4_1:70500857-70500876 | None:intergenic | 65.0% |
| CGGAAATCAGCTCCGGCGAC+CGG | + | chr4_1:70500859-70500878 | Msa0568850:CDS | 65.0% | |
| AAATCAGCTCCGGCGACCGG+AGG | + | chr4_1:70500862-70500881 | Msa0568850:CDS | 65.0% | |
| TCGCAAGTCCACCGGCGGCA+AGG | + | chr4_1:70500804-70500823 | Msa0568850:CDS | 70.0% | |
| CTTCCGGGGAGCCTTGCCGC+CGG | - | chr4_1:70500818-70500837 | None:intergenic | 75.0% | |
| ! | CCACCGGCGGCAAGGCTCCC+CGG | + | chr4_1:70500812-70500831 | Msa0568850:CDS | 80.0% |
| ! | CCGGGGAGCCTTGCCGCCGG+TGG | - | chr4_1:70500815-70500834 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 70500781 | 70501191 | 70500781 | ID=Msa0568850;Name=Msa0568850 |
| chr4_1 | mRNA | 70500781 | 70501191 | 70500781 | ID=Msa0568850-mRNA-1;Parent=Msa0568850;Name=Msa0568850-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr4_1 | exon | 70500781 | 70501191 | 70500781 | ID=Msa0568850-mRNA-1:exon:23807;Parent=Msa0568850-mRNA-1 |
| chr4_1 | CDS | 70500781 | 70501191 | 70500781 | ID=Msa0568850-mRNA-1:cds;Parent=Msa0568850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
